CG5776 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG5776
Entrez Gene ID	34680
Full Name	CG5776 gene product from transcript CG5776-RB
Synonyms	Dmel\CG5776
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 33F3-33F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273491.2	NP_001260420.1	uncharacterized protein, isoform B
NM_135741.4	NP_609585.1	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Spata5	NP_001156983.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009548	XP_001688268.1
Canis lupus familiaris (dog)	SPATA5	XP_540960.2
Homo sapiens (human)	SPATA5	NP_660208.2
Pan troglodytes (chimpanzee)	SPATA5	XP_517427.2
Gallus gallus (chicken)	SPATA5	XP_004941261.1
Danio rerio (zebrafish)	LOC100332622	XP_002663242.3
Eremothecium gossypii	AGOS_AER065C	NP_984925.2
Xenopus tropicalis (tropical clawed frog)	spata5	XP_004911173.1
	SPATA5	XP_001104133.1
Bos taurus (cattle)	SPATA5	XP_002694445.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012655	XP_001230510.1
Rattus norvegicus (Norway rat)	Spata5	NP_001102019.1
Drosophila melanogaster (fruit fly)	CG5776	NP_001260420.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG5776 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG5776 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14560	NM_001273491.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5776), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa14560	NM_135741.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5776), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG5776 ( NM_001273491.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260420.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG5776), mRNA.

Price & Availability↑

CloneID	OFa14560
Clone ID Related Accession (Same CDS sequence)	NM_001273491.2 , NM_135741.4
Accession Version	NM_001273491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273491.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCAA AGTCAAGCAG CAAGAAAAAC CAGGTTACTT GGTACCACTG CGAGTCCTGC 
GGCGTCCACA TTCCCTCGAA AGCGAGAGAT AACCACGAGG GCTTATGCTT CGCCATCAGC 
CAGGATGATG TTGGTCCCGA TTCTGAGGCG GAGTACGTTC GCAGTGGAGC AATCTATACG 
AGAAGTCTTC AACAGCGTAA TTTCGAGGTG GAGTCTCTGA AGGATCTGCC CACCAAGTAT 
GCCAATATGC TAGTCTTCGT CTCCGAGGGT GCGATGCAAT TGGCACAGCT ACACATTGGA 
CAACATGTGG TGCTGGAAGC TCCATCGACG GCGGAGCAGC CGCTGGTTAG GATTGTATGG 
CCCACATCAG AGCAATTCCT CACCACAGTA TTTGTGAGCG AAGGGGATTT CAAACTCCAT 
TGCACACAAC TTCGAGGAAA GCTACTCAAG ATCTCTGCCT TGCATCCCAG TCGCCTCACT 
GCGGCTGCCA GCATTTCCCT GAAGCATGTA AACACCACAG AGACCTCCCT GAAGTCAGTA 
CAACTGCAGG ACGCCATTGC CCTTCTCAAA AGAGACATGG TCAATCGGGT ATTCTACAAG 
GACAGTCTGA TCCACATGAA TTTCTTTAAC AAATCGCTGA CTTTTCGCCT GGAGCGATGG 
CAGAGCACAG TGGAAGAGGC TCTAGCTGGT CTGAGTCTCG ACTCCAAACC CTTGCAGTTC 
ATCCAGGTTA CCAACGTAAC CAAGCTCCAC CTGATAACCG ACGATGCAAA TCAGCAGGAG 
GAGGAGCAAA AAATAAGTCA CAGGATCACC AAATGCCAAA TAGGCGGGCT GGATAGGCAA 
TTGCAACTGG TTGAGGAGAG TATGGAGTAC GCTTTGGGAT TCCGAACGTT GCCTGCAGGC 
CTTAGGGTTT CCCGCGGACT GCTTCTCTAT GGCGCCACTG GCTGTGGCAA GTCCATGGTG 
CTGGAAGCCA TGTGCGCAGT GGCCGAGGAA CGTAGCCAAG GACATGTCCA GCTTATACGC 
ATAAACAGCG GCGAAGTGTA CAGCAAATTC CTGGGCGAAA CGGAACAGAA ACTTGGTGCT 
ATATTTGAGC GTGCCTACAA TCACTATCCG CATCCAACGT TGCTGCTGAT TGAGGATGTG 
CACAACTTGT GTCCCAAGCA AGAAAACAGC GATCTTGTCA AGCGCGTCTC ATTGGCATTC 
TTATCCCTGC TGGATCAGCT AAGCTCTCCC AGCCAGCTAA AGGGAAGCAA AACATTTGTC 
CTGGCCACTA GTTCTCAGAT TGATACCCTG CATCCGAGCA TTCGGCGAGC TGGTCGATTG 
GACAATGAAG TGGAGTTGGG TGCACCCAGC TCTCAGGCAA GGCTGGAAAT TGTACGATGC 
CTCATCAAGT CAGTGGAGCA TCAGTTGAGC GATGAGGAAG TGGAGCACGT TGCATCTATT 
ACCCATGGCT ACGTGGGAGC AGACTTAGCA AATCTCGTTT ACGCTGCCAT GCTGCAGGCG 
CAACCGAATC CTCTACAGAT GCCGCATCTA CAGGCGGCTT TGACCCGCAT TAAACCATCG 
GCGATGCGTG AAGTTCTCAT CGAGTGTCCC AATGTGCAGT GGTCCGACAT TGGCGGTCAA 
TCCGAGCTAC GTTTGGCCAT GCAGCAGGCC ATCGAGTGGC CACTGCTGCA CGCGGATAAG 
TTCCAACGCC TGGGTATCAA GCCACCCAGG GGTATCCTCA TGTTTGGACC GCCAGGCTGC 
TCGAAAACCA TGATTGCCAA GGCCCTGGCC ACCGAGAGCA AGCTTAATTT TCTGTCCATC 
AAGGGACCAG AGCTCTTTTC CATGTGGGTG GGCGAATCGG AGCGAGCGGT GCGTGAGGTC 
TTTCGAAAGG CTCGCCAGGT GGCACCCGCC ATAGTTTTCT TCGACGAAAT CGACGCCATC 
GGTGGCGAGC GTTCGGAGGG CGACGGATCC AGCTCTGGTT CGTCCGTAAA AGAGCGCGTC 
CTCACGCAAC TGCTCACCGA ACTGGATGGC GTGGAGGCAC TGCAAAATGT CACCATAGTC 
GCTGCCACCA ATCGTCCAGA TATGATCGAC AAGGCACTGC TTCGTCCAGG CCGAATTGAT 
CGAATTCTCT ACGTGGGACT ACCGCAATGT GAAGCACGTA GAGAGATCTT AAAGATCAAA 
CTGCGTGCCA TGCCGATATC AAACGATGTG GACATGGAGA AGTTGGTGCA GCTGACGGAG 
GGATATTCCG GTGCAGAAAT ACAAGCCGTA TGCCATGAGG CTGCCCTGAG AGCCCTGGAG 
CAAAGCTTCG AAGCGGAGGA TGTCAAGTGG ACGGACTTCG AGCACGCCTT GAAGGCGGTG 
CCTCCGCGGA CTTCTCCAGA GTTGTTGAAA CTCTACGAAG ATTACCTCAA ACGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260420.1
CDS	52..2451
Translation	MPPKSSSKKNQVTWYHCESCGVHIPSKARDNHEGLCFAISQDDVGPDSEAEYVRSGAIYTRSLQQRNFEVESLKDLPTKYANMLVFVSEGAMQLAQLHIGQHVVLEAPSTAEQPLVRIVWPTSEQFLTTVFVSEGDFKLHCTQLRGKLLKISALHPSRLTAAASISLKHVNTTETSLKSVQLQDAIALLKRDMVNRVFYKDSLIHMNFFNKSLTFRLERWQSTVEEALAGLSLDSKPLQFIQVTNVTKLHLITDDANQQEEEQKISHRITKCQIGGLDRQLQLVEESMEYALGFRTLPAGLRVSRGLLLYGATGCGKSMVLEAMCAVAEERSQGHVQLIRINSGEVYSKFLGETEQKLGAIFERAYNHYPHPTLLLIEDVHNLCPKQENSDLVKRVSLAFLSLLDQLSSPSQLKGSKTFVLATSSQIDTLHPSIRRAGRLDNEVELGAPSSQARLEIVRCLIKSVEHQLSDEEVEHVASITHGYVGADLANLVYAAMLQAQPNPLQMPHLQAALTRIKPSAMREVLIECPNVQWSDIGGQSELRLAMQQAIEWPLLHADKFQRLGIKPPRGILMFGPPGCSKTMIAKALATESKLNFLSIKGPELFSMWVGESERAVREVFRKARQVAPAIVFFDEIDAIGGERSEGDGSSSGSSVKERVLTQLLTELDGVEALQNVTIVAATNRPDMIDKALLRPGRIDRILYVGLPQCEARREILKIKLRAMPISNDVDMEKLVQLTEGYSGAEIQAVCHEAALRALEQSFEAEDVKWTDFEHALKAVPPRTSPELLKLYEDYLKRK

Target ORF information:

RefSeq Version	NM_001273491.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5776), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273491.2

ORF Insert Sequence:

ATGCCGCCAA AGTCAAGCAG CAAGAAAAAC CAGGTTACTT GGTACCACTG CGAGTCCTGC 
GGCGTCCACA TTCCCTCGAA AGCGAGAGAT AACCACGAGG GCTTATGCTT CGCCATCAGC 
CAGGATGATG TTGGTCCCGA TTCTGAGGCG GAGTACGTTC GCAGTGGAGC AATCTATACG 
AGAAGTCTTC AACAGCGTAA TTTCGAGGTG GAGTCTCTGA AGGATCTGCC CACCAAGTAT 
GCCAATATGC TAGTCTTCGT CTCCGAGGGT GCGATGCAAT TGGCACAGCT ACACATTGGA 
CAACATGTGG TGCTGGAAGC TCCATCGACG GCGGAGCAGC CGCTGGTTAG GATTGTATGG 
CCCACATCAG AGCAATTCCT CACCACAGTA TTTGTGAGCG AAGGGGATTT CAAACTCCAT 
TGCACACAAC TTCGAGGAAA GCTACTCAAG ATCTCTGCCT TGCATCCCAG TCGCCTCACT 
GCGGCTGCCA GCATTTCCCT GAAGCATGTA AACACCACAG AGACCTCCCT GAAGTCAGTA 
CAACTGCAGG ACGCCATTGC CCTTCTCAAA AGAGACATGG TCAATCGGGT ATTCTACAAG 
GACAGTCTGA TCCACATGAA TTTCTTTAAC AAATCGCTGA CTTTTCGCCT GGAGCGATGG 
CAGAGCACAG TGGAAGAGGC TCTAGCTGGT CTGAGTCTCG ACTCCAAACC CTTGCAGTTC 
ATCCAGGTTA CCAACGTAAC CAAGCTCCAC CTGATAACCG ACGATGCAAA TCAGCAGGAG 
GAGGAGCAAA AAATAAGTCA CAGGATCACC AAATGCCAAA TAGGCGGGCT GGATAGGCAA 
TTGCAACTGG TTGAGGAGAG TATGGAGTAC GCTTTGGGAT TCCGAACGTT GCCTGCAGGC 
CTTAGGGTTT CCCGCGGACT GCTTCTCTAT GGCGCCACTG GCTGTGGCAA GTCCATGGTG 
CTGGAAGCCA TGTGCGCAGT GGCCGAGGAA CGTAGCCAAG GACATGTCCA GCTTATACGC 
ATAAACAGCG GCGAAGTGTA CAGCAAATTC CTGGGCGAAA CGGAACAGAA ACTTGGTGCT 
ATATTTGAGC GTGCCTACAA TCACTATCCG CATCCAACGT TGCTGCTGAT TGAGGATGTG 
CACAACTTGT GTCCCAAGCA AGAAAACAGC GATCTTGTCA AGCGCGTCTC ATTGGCATTC 
TTATCCCTGC TGGATCAGCT AAGCTCTCCC AGCCAGCTAA AGGGAAGCAA AACATTTGTC 
CTGGCCACTA GTTCTCAGAT TGATACCCTG CATCCGAGCA TTCGGCGAGC TGGTCGATTG 
GACAATGAAG TGGAGTTGGG TGCACCCAGC TCTCAGGCAA GGCTGGAAAT TGTACGATGC 
CTCATCAAGT CAGTGGAGCA TCAGTTGAGC GATGAGGAAG TGGAGCACGT TGCATCTATT 
ACCCATGGCT ACGTGGGAGC AGACTTAGCA AATCTCGTTT ACGCTGCCAT GCTGCAGGCG 
CAACCGAATC CTCTACAGAT GCCGCATCTA CAGGCGGCTT TGACCCGCAT TAAACCATCG 
GCGATGCGTG AAGTTCTCAT CGAGTGTCCC AATGTGCAGT GGTCCGACAT TGGCGGTCAA 
TCCGAGCTAC GTTTGGCCAT GCAGCAGGCC ATCGAGTGGC CACTGCTGCA CGCGGATAAG 
TTCCAACGCC TGGGTATCAA GCCACCCAGG GGTATCCTCA TGTTTGGACC GCCAGGCTGC 
TCGAAAACCA TGATTGCCAA GGCCCTGGCC ACCGAGAGCA AGCTTAATTT TCTGTCCATC 
AAGGGACCAG AGCTCTTTTC CATGTGGGTG GGCGAATCGG AGCGAGCGGT GCGTGAGGTC 
TTTCGAAAGG CTCGCCAGGT GGCACCCGCC ATAGTTTTCT TCGACGAAAT CGACGCCATC 
GGTGGCGAGC GTTCGGAGGG CGACGGATCC AGCTCTGGTT CGTCCGTAAA AGAGCGCGTC 
CTCACGCAAC TGCTCACCGA ACTGGATGGC GTGGAGGCAC TGCAAAATGT CACCATAGTC 
GCTGCCACCA ATCGTCCAGA TATGATCGAC AAGGCACTGC TTCGTCCAGG CCGAATTGAT 
CGAATTCTCT ACGTGGGACT ACCGCAATGT GAAGCACGTA GAGAGATCTT AAAGATCAAA 
CTGCGTGCCA TGCCGATATC AAACGATGTG GACATGGAGA AGTTGGTGCA GCTGACGGAG 
GGATATTCCG GTGCAGAAAT ACAAGCCGTA TGCCATGAGG CTGCCCTGAG AGCCCTGGAG 
CAAAGCTTCG AAGCGGAGGA TGTCAAGTGG ACGGACTTCG AGCACGCCTT GAAGGCGGTG 
CCTCCGCGGA CTTCTCCAGA GTTGTTGAAA CTCTACGAAG ATTACCTCAA ACGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG5776 ( NM_135741.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609585.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG5776), mRNA.

Price & Availability↑

CloneID	OFa14560
Clone ID Related Accession (Same CDS sequence)	NM_001273491.2 , NM_135741.4
Accession Version	NM_135741.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135741.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCAA AGTCAAGCAG CAAGAAAAAC CAGGTTACTT GGTACCACTG CGAGTCCTGC 
GGCGTCCACA TTCCCTCGAA AGCGAGAGAT AACCACGAGG GCTTATGCTT CGCCATCAGC 
CAGGATGATG TTGGTCCCGA TTCTGAGGCG GAGTACGTTC GCAGTGGAGC AATCTATACG 
AGAAGTCTTC AACAGCGTAA TTTCGAGGTG GAGTCTCTGA AGGATCTGCC CACCAAGTAT 
GCCAATATGC TAGTCTTCGT CTCCGAGGGT GCGATGCAAT TGGCACAGCT ACACATTGGA 
CAACATGTGG TGCTGGAAGC TCCATCGACG GCGGAGCAGC CGCTGGTTAG GATTGTATGG 
CCCACATCAG AGCAATTCCT CACCACAGTA TTTGTGAGCG AAGGGGATTT CAAACTCCAT 
TGCACACAAC TTCGAGGAAA GCTACTCAAG ATCTCTGCCT TGCATCCCAG TCGCCTCACT 
GCGGCTGCCA GCATTTCCCT GAAGCATGTA AACACCACAG AGACCTCCCT GAAGTCAGTA 
CAACTGCAGG ACGCCATTGC CCTTCTCAAA AGAGACATGG TCAATCGGGT ATTCTACAAG 
GACAGTCTGA TCCACATGAA TTTCTTTAAC AAATCGCTGA CTTTTCGCCT GGAGCGATGG 
CAGAGCACAG TGGAAGAGGC TCTAGCTGGT CTGAGTCTCG ACTCCAAACC CTTGCAGTTC 
ATCCAGGTTA CCAACGTAAC CAAGCTCCAC CTGATAACCG ACGATGCAAA TCAGCAGGAG 
GAGGAGCAAA AAATAAGTCA CAGGATCACC AAATGCCAAA TAGGCGGGCT GGATAGGCAA 
TTGCAACTGG TTGAGGAGAG TATGGAGTAC GCTTTGGGAT TCCGAACGTT GCCTGCAGGC 
CTTAGGGTTT CCCGCGGACT GCTTCTCTAT GGCGCCACTG GCTGTGGCAA GTCCATGGTG 
CTGGAAGCCA TGTGCGCAGT GGCCGAGGAA CGTAGCCAAG GACATGTCCA GCTTATACGC 
ATAAACAGCG GCGAAGTGTA CAGCAAATTC CTGGGCGAAA CGGAACAGAA ACTTGGTGCT 
ATATTTGAGC GTGCCTACAA TCACTATCCG CATCCAACGT TGCTGCTGAT TGAGGATGTG 
CACAACTTGT GTCCCAAGCA AGAAAACAGC GATCTTGTCA AGCGCGTCTC ATTGGCATTC 
TTATCCCTGC TGGATCAGCT AAGCTCTCCC AGCCAGCTAA AGGGAAGCAA AACATTTGTC 
CTGGCCACTA GTTCTCAGAT TGATACCCTG CATCCGAGCA TTCGGCGAGC TGGTCGATTG 
GACAATGAAG TGGAGTTGGG TGCACCCAGC TCTCAGGCAA GGCTGGAAAT TGTACGATGC 
CTCATCAAGT CAGTGGAGCA TCAGTTGAGC GATGAGGAAG TGGAGCACGT TGCATCTATT 
ACCCATGGCT ACGTGGGAGC AGACTTAGCA AATCTCGTTT ACGCTGCCAT GCTGCAGGCG 
CAACCGAATC CTCTACAGAT GCCGCATCTA CAGGCGGCTT TGACCCGCAT TAAACCATCG 
GCGATGCGTG AAGTTCTCAT CGAGTGTCCC AATGTGCAGT GGTCCGACAT TGGCGGTCAA 
TCCGAGCTAC GTTTGGCCAT GCAGCAGGCC ATCGAGTGGC CACTGCTGCA CGCGGATAAG 
TTCCAACGCC TGGGTATCAA GCCACCCAGG GGTATCCTCA TGTTTGGACC GCCAGGCTGC 
TCGAAAACCA TGATTGCCAA GGCCCTGGCC ACCGAGAGCA AGCTTAATTT TCTGTCCATC 
AAGGGACCAG AGCTCTTTTC CATGTGGGTG GGCGAATCGG AGCGAGCGGT GCGTGAGGTC 
TTTCGAAAGG CTCGCCAGGT GGCACCCGCC ATAGTTTTCT TCGACGAAAT CGACGCCATC 
GGTGGCGAGC GTTCGGAGGG CGACGGATCC AGCTCTGGTT CGTCCGTAAA AGAGCGCGTC 
CTCACGCAAC TGCTCACCGA ACTGGATGGC GTGGAGGCAC TGCAAAATGT CACCATAGTC 
GCTGCCACCA ATCGTCCAGA TATGATCGAC AAGGCACTGC TTCGTCCAGG CCGAATTGAT 
CGAATTCTCT ACGTGGGACT ACCGCAATGT GAAGCACGTA GAGAGATCTT AAAGATCAAA 
CTGCGTGCCA TGCCGATATC AAACGATGTG GACATGGAGA AGTTGGTGCA GCTGACGGAG 
GGATATTCCG GTGCAGAAAT ACAAGCCGTA TGCCATGAGG CTGCCCTGAG AGCCCTGGAG 
CAAAGCTTCG AAGCGGAGGA TGTCAAGTGG ACGGACTTCG AGCACGCCTT GAAGGCGGTG 
CCTCCGCGGA CTTCTCCAGA GTTGTTGAAA CTCTACGAAG ATTACCTCAA ACGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609585.1
CDS	52..2451
Translation	MPPKSSSKKNQVTWYHCESCGVHIPSKARDNHEGLCFAISQDDVGPDSEAEYVRSGAIYTRSLQQRNFEVESLKDLPTKYANMLVFVSEGAMQLAQLHIGQHVVLEAPSTAEQPLVRIVWPTSEQFLTTVFVSEGDFKLHCTQLRGKLLKISALHPSRLTAAASISLKHVNTTETSLKSVQLQDAIALLKRDMVNRVFYKDSLIHMNFFNKSLTFRLERWQSTVEEALAGLSLDSKPLQFIQVTNVTKLHLITDDANQQEEEQKISHRITKCQIGGLDRQLQLVEESMEYALGFRTLPAGLRVSRGLLLYGATGCGKSMVLEAMCAVAEERSQGHVQLIRINSGEVYSKFLGETEQKLGAIFERAYNHYPHPTLLLIEDVHNLCPKQENSDLVKRVSLAFLSLLDQLSSPSQLKGSKTFVLATSSQIDTLHPSIRRAGRLDNEVELGAPSSQARLEIVRCLIKSVEHQLSDEEVEHVASITHGYVGADLANLVYAAMLQAQPNPLQMPHLQAALTRIKPSAMREVLIECPNVQWSDIGGQSELRLAMQQAIEWPLLHADKFQRLGIKPPRGILMFGPPGCSKTMIAKALATESKLNFLSIKGPELFSMWVGESERAVREVFRKARQVAPAIVFFDEIDAIGGERSEGDGSSSGSSVKERVLTQLLTELDGVEALQNVTIVAATNRPDMIDKALLRPGRIDRILYVGLPQCEARREILKIKLRAMPISNDVDMEKLVQLTEGYSGAEIQAVCHEAALRALEQSFEAEDVKWTDFEHALKAVPPRTSPELLKLYEDYLKRK

Target ORF information:

RefSeq Version	NM_135741.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5776), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135741.4

ORF Insert Sequence:

ATGCCGCCAA AGTCAAGCAG CAAGAAAAAC CAGGTTACTT GGTACCACTG CGAGTCCTGC 
GGCGTCCACA TTCCCTCGAA AGCGAGAGAT AACCACGAGG GCTTATGCTT CGCCATCAGC 
CAGGATGATG TTGGTCCCGA TTCTGAGGCG GAGTACGTTC GCAGTGGAGC AATCTATACG 
AGAAGTCTTC AACAGCGTAA TTTCGAGGTG GAGTCTCTGA AGGATCTGCC CACCAAGTAT 
GCCAATATGC TAGTCTTCGT CTCCGAGGGT GCGATGCAAT TGGCACAGCT ACACATTGGA 
CAACATGTGG TGCTGGAAGC TCCATCGACG GCGGAGCAGC CGCTGGTTAG GATTGTATGG 
CCCACATCAG AGCAATTCCT CACCACAGTA TTTGTGAGCG AAGGGGATTT CAAACTCCAT 
TGCACACAAC TTCGAGGAAA GCTACTCAAG ATCTCTGCCT TGCATCCCAG TCGCCTCACT 
GCGGCTGCCA GCATTTCCCT GAAGCATGTA AACACCACAG AGACCTCCCT GAAGTCAGTA 
CAACTGCAGG ACGCCATTGC CCTTCTCAAA AGAGACATGG TCAATCGGGT ATTCTACAAG 
GACAGTCTGA TCCACATGAA TTTCTTTAAC AAATCGCTGA CTTTTCGCCT GGAGCGATGG 
CAGAGCACAG TGGAAGAGGC TCTAGCTGGT CTGAGTCTCG ACTCCAAACC CTTGCAGTTC 
ATCCAGGTTA CCAACGTAAC CAAGCTCCAC CTGATAACCG ACGATGCAAA TCAGCAGGAG 
GAGGAGCAAA AAATAAGTCA CAGGATCACC AAATGCCAAA TAGGCGGGCT GGATAGGCAA 
TTGCAACTGG TTGAGGAGAG TATGGAGTAC GCTTTGGGAT TCCGAACGTT GCCTGCAGGC 
CTTAGGGTTT CCCGCGGACT GCTTCTCTAT GGCGCCACTG GCTGTGGCAA GTCCATGGTG 
CTGGAAGCCA TGTGCGCAGT GGCCGAGGAA CGTAGCCAAG GACATGTCCA GCTTATACGC 
ATAAACAGCG GCGAAGTGTA CAGCAAATTC CTGGGCGAAA CGGAACAGAA ACTTGGTGCT 
ATATTTGAGC GTGCCTACAA TCACTATCCG CATCCAACGT TGCTGCTGAT TGAGGATGTG 
CACAACTTGT GTCCCAAGCA AGAAAACAGC GATCTTGTCA AGCGCGTCTC ATTGGCATTC 
TTATCCCTGC TGGATCAGCT AAGCTCTCCC AGCCAGCTAA AGGGAAGCAA AACATTTGTC 
CTGGCCACTA GTTCTCAGAT TGATACCCTG CATCCGAGCA TTCGGCGAGC TGGTCGATTG 
GACAATGAAG TGGAGTTGGG TGCACCCAGC TCTCAGGCAA GGCTGGAAAT TGTACGATGC 
CTCATCAAGT CAGTGGAGCA TCAGTTGAGC GATGAGGAAG TGGAGCACGT TGCATCTATT 
ACCCATGGCT ACGTGGGAGC AGACTTAGCA AATCTCGTTT ACGCTGCCAT GCTGCAGGCG 
CAACCGAATC CTCTACAGAT GCCGCATCTA CAGGCGGCTT TGACCCGCAT TAAACCATCG 
GCGATGCGTG AAGTTCTCAT CGAGTGTCCC AATGTGCAGT GGTCCGACAT TGGCGGTCAA 
TCCGAGCTAC GTTTGGCCAT GCAGCAGGCC ATCGAGTGGC CACTGCTGCA CGCGGATAAG 
TTCCAACGCC TGGGTATCAA GCCACCCAGG GGTATCCTCA TGTTTGGACC GCCAGGCTGC 
TCGAAAACCA TGATTGCCAA GGCCCTGGCC ACCGAGAGCA AGCTTAATTT TCTGTCCATC 
AAGGGACCAG AGCTCTTTTC CATGTGGGTG GGCGAATCGG AGCGAGCGGT GCGTGAGGTC 
TTTCGAAAGG CTCGCCAGGT GGCACCCGCC ATAGTTTTCT TCGACGAAAT CGACGCCATC 
GGTGGCGAGC GTTCGGAGGG CGACGGATCC AGCTCTGGTT CGTCCGTAAA AGAGCGCGTC 
CTCACGCAAC TGCTCACCGA ACTGGATGGC GTGGAGGCAC TGCAAAATGT CACCATAGTC 
GCTGCCACCA ATCGTCCAGA TATGATCGAC AAGGCACTGC TTCGTCCAGG CCGAATTGAT 
CGAATTCTCT ACGTGGGACT ACCGCAATGT GAAGCACGTA GAGAGATCTT AAAGATCAAA 
CTGCGTGCCA TGCCGATATC AAACGATGTG GACATGGAGA AGTTGGTGCA GCTGACGGAG 
GGATATTCCG GTGCAGAAAT ACAAGCCGTA TGCCATGAGG CTGCCCTGAG AGCCCTGGAG 
CAAAGCTTCG AAGCGGAGGA TGTCAAGTGG ACGGACTTCG AGCACGCCTT GAAGGCGGTG 
CCTCCGCGGA CTTCTCCAGA GTTGTTGAAA CTCTACGAAG ATTACCTCAA ACGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG5776 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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