Ced-12 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ced-12
Entrez Gene ID	34633
Full Name	CG5336 gene product from transcript CG5336-RA
Synonyms	CG5336,Ced12,Dced-12,Dmel\CG5336,ELMO,Elmo,ced-12,ced12,dCed-12,dCed12,dced-12,elmo
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 33C4-33C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_135704.3	NP_609548.1	Ced-12

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ELMO1	XP_005628778.1
Mus musculus (house mouse)	Elmo1	NP_525027.1
Rattus norvegicus (Norway rat)	Elmo1	NP_001101885.1
Xenopus tropicalis (tropical clawed frog)	elmo2	XP_002939462.1
Bos taurus (cattle)	ELMO1	NP_001106698.1
Gallus gallus (chicken)	ELMO1	NP_001026165.1
Drosophila melanogaster (fruit fly)	Ced-12	NP_609548.1
Homo sapiens (human)	ELMO1	NP_001193409.1
Danio rerio (zebrafish)	elmo1	NP_998256.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009236	XP_320013.2
Pan troglodytes (chimpanzee)	ELMO1	XP_001170197.1
	ELMO1	XP_002803364.1
Caenorhabditis elegans (roundworm)	ced-12	NP_492693.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results show that integrins function as upstream signals to mediate Dim7-Elmo enrichment to the muscle attachment sites .
Title: Drosophila importin-7 functions upstream of the Elmo signaling module to mediate the formation and stability of muscle attachments.

identified and characterized the role of Drosophila Sponge (Spg), the vertebrate DOCK3/DOCK4 counterpart as an ELMO-interacting protein
Title: The DOCK protein sponge binds to ELMO and functions in Drosophila embryonic CNS development.

Overexpression of MBC and ELMO in the embryonic mesoderm causes defects in myoblast fusion reminiscent of those seen with constitutively-activated Rac1
Title: Drosophila ELMO/CED-12 interacts with Myoblast city to direct myoblast fusion and ommatidial organization.

The following Ced-12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ced-12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14510	NM_135704.3 Latest version!	Drosophila melanogaster Ced-12 (Ced-12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ced-12 ( NM_135704.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609548.1 Drosophila melanogaster Ced-12 (Ced-12), mRNA.

Price & Availability↑

CloneID	OFa14510
Clone ID Related Accession (Same CDS sequence)	NM_135704.3
Accession Version	NM_135704.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ced-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135704.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATACCAA AAAAGACGAC GGTTAAGGAT TCACACATTG TGAAAATAGC CGTGGAGCGA 
GAGGACCATA TAGCGCAGTT GATAAATCTG GATCAAAGGC ATCCGCTGGC AAGTAAAATC 
CAGGAAATTT GCAATGGTTG GTCCATCAGC GATCACCAGA ACTATGCACT CCAGTTCTAC 
GAGCCCACTA ACCGAAAGTA CGTGACCGAA AAGAATCGCA ATGAGATCAA GAATGGCTCC 
GTACTGCAGC TGCAGTATTC GCCATCCAAG ACTGCCTGCG ATGCCATGGA GGTGCTGCTC 
AATGGCAGTC CACAGGAGAA GGCGCTGCGT CTAAAGGAGC TCACTTCCCT AAGCACCGAT 
CACACATTTG CCCTGGAGTT CATCAAGGAG AAGGGTCTCG ACACACTCAT TAAGATGATT 
GAGGATGGCG GCCAGACCAA CGAAGACATT CTGAAATACA GCCTGGCCAG CTTTGTGGAG 
CTGATGGAGC ACGGCACGGT GTCCTGGGAG GTGCCGGAAA ACTCATTCGT GGCCCGCAAC 
ATTGAGATCG TGCGAAACTT TCAGAAATAT CCTACGAACT GTGGTGAGAG CGCCCTGTCC 
AACTTGGAGA ACATTGTTAT GTGCAGCAAT AAACATGTGC TTGTTGCCGA GGACATCAAG 
CTGCAGGATA TTCTCCGATT GCTTCAGGAT GTCAATTCGC CGGTGATGCG ACAAAATGCC 
ATAGCCCTGC TGAACGCTCT GTTCGTCAAA GCAGACGAGG GCCGCCGCAG GACCATTGCT 
CAAACTATTA GTGCCAAGCA GTTCCGCCTG GCCCTTATCG GCAATGGACT GGGCACAGAG 
ATGACCCACC AGCTGTACGT GCTGCAAACG TTGACTCTCG GCCTGTTGGA GAAGCGCATG 
CGTATGAAGA TGAACGCGCA GGACCAGGAC GCGCACGAGA AGATCAAGGA GCTGCGGCGC 
ATTGCCTTCG ACGATAATAC CAACGCTTTG AATCAAAACG ACGATCACAT CCGTCGTGGC 
GGTGGCTCCG GAGCTGGCAA TGTCAACTTC TCGCAGTACT ACAAAAAGCT GGGCTTCAAG 
TGCGATATTA ACCCTGCCCA GGATTTCATT GAGACGCCGC CGGGTATATT AGCGTTGGAT 
TGCATGGTGT ATTTTGCACG CAACTACACG CAACAGTATG CGAAGATCGT CAGGGAGAAC 
TCCTGCCGTG CGGATGAGCA CGAGTGTCCC TTCGGACGCA CCTCCATCGA ATTGGTCAAG 
GTGCTATGTG ACATCCTTCG CATCGGCGAG CCACCAGCCG AGCAATCCGG TGACTTTCAG 
CCCATGTTCT TTACGCACGA TTCGCCGTTC GAGGAATTCT TTTGCATATG CGTCATTACG 
CTGAATCGCA CATGGAAGGA TATGCGTGCC ACTGCCGAGG ACTTCAGCAC AACCTTCAGC 
GTGGTGCGGG AGCAGATCCA GCGCACTCTA AAGGGCAGAC CCGAGAACCT GGACGATTTC 
CGAAACAAGA TCGCTTTGTT GACCTATCAG CAGATCACAA CACTGCGCCA GCAGGAGCGC 
ACTTCGAAGG AGGAGTGTGA TTCCACAGCT TCGGCGATTG TTAAGCTCAA AGAGAAGATA 
TCACCGCAAA TTCTTGAGCT AATCAAGCAG CAACGGCTCT CTTTTCTGGT GGAAGGTACA 
CATTTTGCAA AATACTTGCG AGGCACTCGA ACAAAGGACA AGTTCTGGTA CGCTCGTCTT 
TCGCCGAATC ACAAGGTGAT TCATTACGGA GATTGCGACG AGAAGACAAT ACCCACCATG 
GAGGAGTTGC CCAAGAAACT GCCCATCAGT GAGATTAAGC AATTGCTAGA GGGCAAGGAA 
TGCCCGCATA TGAAGGAAAC TCGCATTCGG AAATCGGCGG TAAACCTGGC CTTTTCCATC 
ACCTTCGAAA ACATGGAGCA CTCTACGTTG GACTTTGTGG CACCCGACGA AAGCATATTC 
AACTACTGGA CGGATGGAAT AAACGCGCTG CTGGGTCAAC CGATGGTCAG CAAGCAAAAG 
AACGAGGACT TCGACACTCT GCTGTCGATG GAGATCAAAT TGCGCCTGCT GGACACGGAA 
GGTGTGGACA TCAGCAAGGA CCCACCGCCA ATACCGGAGG ATCCCGAAAA CTATGATTTT 
TGCTTTGAGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609548.1
CDS	114..2288
Translation	MIPKKTTVKDSHIVKIAVEREDHIAQLINLDQRHPLASKIQEICNGWSISDHQNYALQFYEPTNRKYVTEKNRNEIKNGSVLQLQYSPSKTACDAMEVLLNGSPQEKALRLKELTSLSTDHTFALEFIKEKGLDTLIKMIEDGGQTNEDILKYSLASFVELMEHGTVSWEVPENSFVARNIEIVRNFQKYPTNCGESALSNLENIVMCSNKHVLVAEDIKLQDILRLLQDVNSPVMRQNAIALLNALFVKADEGRRRTIAQTISAKQFRLALIGNGLGTEMTHQLYVLQTLTLGLLEKRMRMKMNAQDQDAHEKIKELRRIAFDDNTNALNQNDDHIRRGGGSGAGNVNFSQYYKKLGFKCDINPAQDFIETPPGILALDCMVYFARNYTQQYAKIVRENSCRADEHECPFGRTSIELVKVLCDILRIGEPPAEQSGDFQPMFFTHDSPFEEFFCICVITLNRTWKDMRATAEDFSTTFSVVREQIQRTLKGRPENLDDFRNKIALLTYQQITTLRQQERTSKEECDSTASAIVKLKEKISPQILELIKQQRLSFLVEGTHFAKYLRGTRTKDKFWYARLSPNHKVIHYGDCDEKTIPTMEELPKKLPISEIKQLLEGKECPHMKETRIRKSAVNLAFSITFENMEHSTLDFVAPDESIFNYWTDGINALLGQPMVSKQKNEDFDTLLSMEIKLRLLDTEGVDISKDPPPIPEDPENYDFCFES

Target ORF information:

RefSeq Version	NM_135704.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ced-12 (Ced-12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135704.3

ORF Insert Sequence:

ATGATACCAA AAAAGACGAC GGTTAAGGAT TCACACATTG TGAAAATAGC CGTGGAGCGA 
GAGGACCATA TAGCGCAGTT GATAAATCTG GATCAAAGGC ATCCGCTGGC AAGTAAAATC 
CAGGAAATTT GCAATGGTTG GTCCATCAGC GATCACCAGA ACTATGCACT CCAGTTCTAC 
GAGCCCACTA ACCGAAAGTA CGTGACCGAA AAGAATCGCA ATGAGATCAA GAATGGCTCC 
GTACTGCAGC TGCAGTATTC GCCATCCAAG ACTGCCTGCG ATGCCATGGA GGTGCTGCTC 
AATGGCAGTC CACAGGAGAA GGCGCTGCGT CTAAAGGAGC TCACTTCCCT AAGCACCGAT 
CACACATTTG CCCTGGAGTT CATCAAGGAG AAGGGTCTCG ACACACTCAT TAAGATGATT 
GAGGATGGCG GCCAGACCAA CGAAGACATT CTGAAATACA GCCTGGCCAG CTTTGTGGAG 
CTGATGGAGC ACGGCACGGT GTCCTGGGAG GTGCCGGAAA ACTCATTCGT GGCCCGCAAC 
ATTGAGATCG TGCGAAACTT TCAGAAATAT CCTACGAACT GTGGTGAGAG CGCCCTGTCC 
AACTTGGAGA ACATTGTTAT GTGCAGCAAT AAACATGTGC TTGTTGCCGA GGACATCAAG 
CTGCAGGATA TTCTCCGATT GCTTCAGGAT GTCAATTCGC CGGTGATGCG ACAAAATGCC 
ATAGCCCTGC TGAACGCTCT GTTCGTCAAA GCAGACGAGG GCCGCCGCAG GACCATTGCT 
CAAACTATTA GTGCCAAGCA GTTCCGCCTG GCCCTTATCG GCAATGGACT GGGCACAGAG 
ATGACCCACC AGCTGTACGT GCTGCAAACG TTGACTCTCG GCCTGTTGGA GAAGCGCATG 
CGTATGAAGA TGAACGCGCA GGACCAGGAC GCGCACGAGA AGATCAAGGA GCTGCGGCGC 
ATTGCCTTCG ACGATAATAC CAACGCTTTG AATCAAAACG ACGATCACAT CCGTCGTGGC 
GGTGGCTCCG GAGCTGGCAA TGTCAACTTC TCGCAGTACT ACAAAAAGCT GGGCTTCAAG 
TGCGATATTA ACCCTGCCCA GGATTTCATT GAGACGCCGC CGGGTATATT AGCGTTGGAT 
TGCATGGTGT ATTTTGCACG CAACTACACG CAACAGTATG CGAAGATCGT CAGGGAGAAC 
TCCTGCCGTG CGGATGAGCA CGAGTGTCCC TTCGGACGCA CCTCCATCGA ATTGGTCAAG 
GTGCTATGTG ACATCCTTCG CATCGGCGAG CCACCAGCCG AGCAATCCGG TGACTTTCAG 
CCCATGTTCT TTACGCACGA TTCGCCGTTC GAGGAATTCT TTTGCATATG CGTCATTACG 
CTGAATCGCA CATGGAAGGA TATGCGTGCC ACTGCCGAGG ACTTCAGCAC AACCTTCAGC 
GTGGTGCGGG AGCAGATCCA GCGCACTCTA AAGGGCAGAC CCGAGAACCT GGACGATTTC 
CGAAACAAGA TCGCTTTGTT GACCTATCAG CAGATCACAA CACTGCGCCA GCAGGAGCGC 
ACTTCGAAGG AGGAGTGTGA TTCCACAGCT TCGGCGATTG TTAAGCTCAA AGAGAAGATA 
TCACCGCAAA TTCTTGAGCT AATCAAGCAG CAACGGCTCT CTTTTCTGGT GGAAGGTACA 
CATTTTGCAA AATACTTGCG AGGCACTCGA ACAAAGGACA AGTTCTGGTA CGCTCGTCTT 
TCGCCGAATC ACAAGGTGAT TCATTACGGA GATTGCGACG AGAAGACAAT ACCCACCATG 
GAGGAGTTGC CCAAGAAACT GCCCATCAGT GAGATTAAGC AATTGCTAGA GGGCAAGGAA 
TGCCCGCATA TGAAGGAAAC TCGCATTCGG AAATCGGCGG TAAACCTGGC CTTTTCCATC 
ACCTTCGAAA ACATGGAGCA CTCTACGTTG GACTTTGTGG CACCCGACGA AAGCATATTC 
AACTACTGGA CGGATGGAAT AAACGCGCTG CTGGGTCAAC CGATGGTCAG CAAGCAAAAG 
AACGAGGACT TCGACACTCT GCTGTCGATG GAGATCAAAT TGCGCCTGCT GGACACGGAA 
GGTGTGGACA TCAGCAAGGA CCCACCGCCA ATACCGGAGG ATCCCGAAAA CTATGATTTT 
TGCTTTGAGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ced-12 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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