Tom70 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tom70
Entrez Gene ID	34618
Full Name	Translocase of outer membrane 70
Synonyms	CG6756,Dmel\CG6756,RE66761p,dtom70
General protein information	Preferred Names Translocase of outer membrane 70 Names CG6756 gene product from transcript CG6756-RB CG6756-PA CG6756-PB CG6756-PC Tom70-PA Tom70-PB Tom70-PC translocase of outer membrane 70
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 33B9-33B10

mRNA and Protein(s)

mRNA	Protein	Name
NM_164985.2	NP_723711.1	translocase of outer membrane 70, isoform B
NM_135692.2	NP_609536.1	translocase of outer membrane 70, isoform A
NM_164986.2	NP_723712.1	translocase of outer membrane 70, isoform C

Pathways from BioSystems

Homologies

	TOMM70A	XP_001092332.1
Danio rerio (zebrafish)	tomm70a	NP_956296.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009497	XP_310198.4
Schizosaccharomyces pombe (fission yeast)	tom70	NP_593767.1
Magnaporthe oryzae (rice blast fungus)	MGG_00979	XP_003717970.1
Mus musculus (house mouse)	Tomm70a	NP_613065.2
Rattus norvegicus (Norway rat)	Tomm70a	NP_997684.1
Gallus gallus (chicken)	TOMM70A	XP_416605.2
Drosophila melanogaster (fruit fly)	Tom70	NP_609536.1
Xenopus tropicalis (tropical clawed frog)	tomm70a	NP_001096527.1
Pan troglodytes (chimpanzee)	TOMM70A	XP_526255.2
Canis lupus familiaris (dog)	TOMM70A	XP_535719.3
Bos taurus (cattle)	TOMM70A	NP_001068796.1
Eremothecium gossypii	AGOS_ABR131W	NP_983078.1
Homo sapiens (human)	TOMM70A	NP_055635.3
Saccharomyces cerevisiae (baker's yeast)	TOM70	NP_014278.3
Kluyveromyces lactis	KLLA0F24046g	XP_456151.1
Neurospora crassa	NCU04245	XP_961236.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tom70 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tom70 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14492	NM_164986.2 Latest version!	Drosophila melanogaster translocase of outer membrane 70, transcript variant C (Tom70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14492	NM_164985.2 Latest version!	Drosophila melanogaster translocase of outer membrane 70, transcript variant B (Tom70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14492	NM_135692.2 Latest version!	Drosophila melanogaster translocase of outer membrane 70, transcript variant A (Tom70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tom70 ( NM_164985.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723711.1 Drosophila melanogaster translocase of outer membrane 70, transcript variant B (Tom70), mRNA.

Price & Availability↑

CloneID	OFa14492
Clone ID Related Accession (Same CDS sequence)	NM_164985.2 , NM_135692.2 , NM_164986.2
Accession Version	NM_164985.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	translocase of outer membrane 70, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_164985.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGATGA CTCTGAACAT TGGCTCGATG AAGCTGACAA AGTGGCAGCT GGCGCTCATC 
CTTGGAACTC CCCTGGCCAT TGGACTGGGC ACCTATGCGG TGAAGAGCTG GACGGCTGCG 
TCCAAGGAGT TGGACGGGGA GAAGAAGAGA CCAAAGGCCA AGATCGAGAA GCAGGCCATC 
TCCCTGGACG GCACCGCACC GGACCAGGAG CTAGAGCGCA ATCAAAAGTC CGCTGAGCTG 
GGCGAGAAGC TGTCGCCTCT TAAGGAGGCC AACAACTACA AGACGGAGGG TAACAACTGC 
TACAGGAATG GGAAGTACGA TGAGGCCATT AAATTCTATG ACAAGGCCAT CGACAAGTGT 
CCCAAGGAGC ACCGCACTGA CATGGCCATC TTCTACCAGA ACCGCGCCGC CTCTTACGAG 
ATGCTCAAGA AGTGGTCCAA CGTCAAGGAG GACTGCACAG CCTCGCTGGA GTTCAATCCT 
CGTTACGCCA AGGCATATTA TCGTCGTGCC CGTGCTCACG AAGCCACCAA GGATATGAAC 
GAGTGCTTGG ACGATGTCAC AGCCACCTGT ATCCTGGAGA TGTTCCAAAA TAATCAAACT 
ATCATGTTCG CTGACCGTGT GCTCAAGGAG ACCGGCCGAC TGGACGCCGA GAAGGGTATG 
CGCAATCGGG TGCCAGTCGT GCCGTCGGCT TGCTTCGTAA ACACCTACAC GCGTTCCTTC 
ATCGCGGATC CGCTGCAGAC CATGAAAGTC CCAGCCCCTA AATCCGATGC ACCCCCAAAG 
GGTTTCCTGC GCGCCCACCT CGCTTTCCTC GAGGAGAAGT ACGAGGAAAT TATTCCCGCC 
TGCACCGAAG AGATCGAATC CTCCGAGGCC GAAGCACAGT ACAAAGTGGA GGCTCTGCTT 
ATGCGGGGAA CTTTCCATCT GCTGTGCGGC TCCTATGTAG AAAGCCAGCA GGACTTTGAT 
GCTATTATAG CTAATGACTA TGCTGATCCC AACTTGCGTG CGTATGCCTA CATAAAGCGG 
GCAGCTCTCT ACATCCAGCT GGATCAGCGC GAAAAGGGTA TAGCCGATTT TGCTGAGGCG 
GAACGCCTGA ACCCAGAGAA CCCAGATGTG TACCACCAGC GCGCCCAGAT ACTTCTGCTG 
CTGGAGCAGA TAGAGCCCGC CTTGGCCGAG TTCGAAAAGG CGGTGAGCAT TGCACCCAAC 
CACGCCATCG CCTTCGTCCA GAAGTGCTAT GCCGAGTACC GACTATCCCT CCTGGCCGGC 
GACCAGCGGC GCTTGGAGAG CGTAATGCAC ACCTTCCAAA ATGCCATCGA GCGTTTCCCG 
AGCTGCGTTG AGTGCTATAG TCTAACGGCT CAGGTGTTGG CCGACCAGCA ACAGTTTACC 
CAGGCGGAGG AATACTACAA AAAGGCCATG GTATTGGCGC CAACAAATCC TGCACTTATC 
GTTCACCAGG CCATCATGGT GCTTCAGTGG CGCGGAGACA TTAATTTGGC TGTCCAGCTG 
CTCAACAAGG CGATCGAAGT AGATCCCAAA TGCGAGCTGG CCTACGAGAC ACTCGGAACA 
GTCGAAGTTC AGCGGGCGCA ACTCACACGG GCCGTCGAGC TTTTTGAAAA GGCACTGCTT 
TATGCCAAGA GTCAGGCGGA GTTGGTGCAC GTTTACTCGC TGCGCAACGC GGCAGTGGCC 
CAAATCAACG TCACCAAAAA GCTGGGTATC GACATGAACT CAGTGTCGGC AATGGCCCAA 
TCCGGATTGA TGGCCCAGGG CGTTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723711.1
CDS	181..1950
Translation	MAMTLNIGSMKLTKWQLALILGTPLAIGLGTYAVKSWTAASKELDGEKKRPKAKIEKQAISLDGTAPDQELERNQKSAELGEKLSPLKEANNYKTEGNNCYRNGKYDEAIKFYDKAIDKCPKEHRTDMAIFYQNRAASYEMLKKWSNVKEDCTASLEFNPRYAKAYYRRARAHEATKDMNECLDDVTATCILEMFQNNQTIMFADRVLKETGRLDAEKGMRNRVPVVPSACFVNTYTRSFIADPLQTMKVPAPKSDAPPKGFLRAHLAFLEEKYEEIIPACTEEIESSEAEAQYKVEALLMRGTFHLLCGSYVESQQDFDAIIANDYADPNLRAYAYIKRAALYIQLDQREKGIADFAEAERLNPENPDVYHQRAQILLLLEQIEPALAEFEKAVSIAPNHAIAFVQKCYAEYRLSLLAGDQRRLESVMHTFQNAIERFPSCVECYSLTAQVLADQQQFTQAEEYYKKAMVLAPTNPALIVHQAIMVLQWRGDINLAVQLLNKAIEVDPKCELAYETLGTVEVQRAQLTRAVELFEKALLYAKSQAELVHVYSLRNAAVAQINVTKKLGIDMNSVSAMAQSGLMAQGVA

Target ORF information:

RefSeq Version	NM_164985.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster translocase of outer membrane 70, transcript variant B (Tom70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164985.2

ORF Insert Sequence:

ATGGCGATGA CTCTGAACAT TGGCTCGATG AAGCTGACAA AGTGGCAGCT GGCGCTCATC 
CTTGGAACTC CCCTGGCCAT TGGACTGGGC ACCTATGCGG TGAAGAGCTG GACGGCTGCG 
TCCAAGGAGT TGGACGGGGA GAAGAAGAGA CCAAAGGCCA AGATCGAGAA GCAGGCCATC 
TCCCTGGACG GCACCGCACC GGACCAGGAG CTAGAGCGCA ATCAAAAGTC CGCTGAGCTG 
GGCGAGAAGC TGTCGCCTCT TAAGGAGGCC AACAACTACA AGACGGAGGG TAACAACTGC 
TACAGGAATG GGAAGTACGA TGAGGCCATT AAATTCTATG ACAAGGCCAT CGACAAGTGT 
CCCAAGGAGC ACCGCACTGA CATGGCCATC TTCTACCAGA ACCGCGCCGC CTCTTACGAG 
ATGCTCAAGA AGTGGTCCAA CGTCAAGGAG GACTGCACAG CCTCGCTGGA GTTCAATCCT 
CGTTACGCCA AGGCATATTA TCGTCGTGCC CGTGCTCACG AAGCCACCAA GGATATGAAC 
GAGTGCTTGG ACGATGTCAC AGCCACCTGT ATCCTGGAGA TGTTCCAAAA TAATCAAACT 
ATCATGTTCG CTGACCGTGT GCTCAAGGAG ACCGGCCGAC TGGACGCCGA GAAGGGTATG 
CGCAATCGGG TGCCAGTCGT GCCGTCGGCT TGCTTCGTAA ACACCTACAC GCGTTCCTTC 
ATCGCGGATC CGCTGCAGAC CATGAAAGTC CCAGCCCCTA AATCCGATGC ACCCCCAAAG 
GGTTTCCTGC GCGCCCACCT CGCTTTCCTC GAGGAGAAGT ACGAGGAAAT TATTCCCGCC 
TGCACCGAAG AGATCGAATC CTCCGAGGCC GAAGCACAGT ACAAAGTGGA GGCTCTGCTT 
ATGCGGGGAA CTTTCCATCT GCTGTGCGGC TCCTATGTAG AAAGCCAGCA GGACTTTGAT 
GCTATTATAG CTAATGACTA TGCTGATCCC AACTTGCGTG CGTATGCCTA CATAAAGCGG 
GCAGCTCTCT ACATCCAGCT GGATCAGCGC GAAAAGGGTA TAGCCGATTT TGCTGAGGCG 
GAACGCCTGA ACCCAGAGAA CCCAGATGTG TACCACCAGC GCGCCCAGAT ACTTCTGCTG 
CTGGAGCAGA TAGAGCCCGC CTTGGCCGAG TTCGAAAAGG CGGTGAGCAT TGCACCCAAC 
CACGCCATCG CCTTCGTCCA GAAGTGCTAT GCCGAGTACC GACTATCCCT CCTGGCCGGC 
GACCAGCGGC GCTTGGAGAG CGTAATGCAC ACCTTCCAAA ATGCCATCGA GCGTTTCCCG 
AGCTGCGTTG AGTGCTATAG TCTAACGGCT CAGGTGTTGG CCGACCAGCA ACAGTTTACC 
CAGGCGGAGG AATACTACAA AAAGGCCATG GTATTGGCGC CAACAAATCC TGCACTTATC 
GTTCACCAGG CCATCATGGT GCTTCAGTGG CGCGGAGACA TTAATTTGGC TGTCCAGCTG 
CTCAACAAGG CGATCGAAGT AGATCCCAAA TGCGAGCTGG CCTACGAGAC ACTCGGAACA 
GTCGAAGTTC AGCGGGCGCA ACTCACACGG GCCGTCGAGC TTTTTGAAAA GGCACTGCTT 
TATGCCAAGA GTCAGGCGGA GTTGGTGCAC GTTTACTCGC TGCGCAACGC GGCAGTGGCC 
CAAATCAACG TCACCAAAAA GCTGGGTATC GACATGAACT CAGTGTCGGC AATGGCCCAA 
TCCGGATTGA TGGCCCAGGG CGTTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tom70 ( NM_135692.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609536.1 Drosophila melanogaster translocase of outer membrane 70, transcript variant A (Tom70), mRNA.

Price & Availability↑

CloneID	OFa14492
Clone ID Related Accession (Same CDS sequence)	NM_164985.2 , NM_135692.2 , NM_164986.2
Accession Version	NM_135692.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	translocase of outer membrane 70, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135692.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGATGA CTCTGAACAT TGGCTCGATG AAGCTGACAA AGTGGCAGCT GGCGCTCATC 
CTTGGAACTC CCCTGGCCAT TGGACTGGGC ACCTATGCGG TGAAGAGCTG GACGGCTGCG 
TCCAAGGAGT TGGACGGGGA GAAGAAGAGA CCAAAGGCCA AGATCGAGAA GCAGGCCATC 
TCCCTGGACG GCACCGCACC GGACCAGGAG CTAGAGCGCA ATCAAAAGTC CGCTGAGCTG 
GGCGAGAAGC TGTCGCCTCT TAAGGAGGCC AACAACTACA AGACGGAGGG TAACAACTGC 
TACAGGAATG GGAAGTACGA TGAGGCCATT AAATTCTATG ACAAGGCCAT CGACAAGTGT 
CCCAAGGAGC ACCGCACTGA CATGGCCATC TTCTACCAGA ACCGCGCCGC CTCTTACGAG 
ATGCTCAAGA AGTGGTCCAA CGTCAAGGAG GACTGCACAG CCTCGCTGGA GTTCAATCCT 
CGTTACGCCA AGGCATATTA TCGTCGTGCC CGTGCTCACG AAGCCACCAA GGATATGAAC 
GAGTGCTTGG ACGATGTCAC AGCCACCTGT ATCCTGGAGA TGTTCCAAAA TAATCAAACT 
ATCATGTTCG CTGACCGTGT GCTCAAGGAG ACCGGCCGAC TGGACGCCGA GAAGGGTATG 
CGCAATCGGG TGCCAGTCGT GCCGTCGGCT TGCTTCGTAA ACACCTACAC GCGTTCCTTC 
ATCGCGGATC CGCTGCAGAC CATGAAAGTC CCAGCCCCTA AATCCGATGC ACCCCCAAAG 
GGTTTCCTGC GCGCCCACCT CGCTTTCCTC GAGGAGAAGT ACGAGGAAAT TATTCCCGCC 
TGCACCGAAG AGATCGAATC CTCCGAGGCC GAAGCACAGT ACAAAGTGGA GGCTCTGCTT 
ATGCGGGGAA CTTTCCATCT GCTGTGCGGC TCCTATGTAG AAAGCCAGCA GGACTTTGAT 
GCTATTATAG CTAATGACTA TGCTGATCCC AACTTGCGTG CGTATGCCTA CATAAAGCGG 
GCAGCTCTCT ACATCCAGCT GGATCAGCGC GAAAAGGGTA TAGCCGATTT TGCTGAGGCG 
GAACGCCTGA ACCCAGAGAA CCCAGATGTG TACCACCAGC GCGCCCAGAT ACTTCTGCTG 
CTGGAGCAGA TAGAGCCCGC CTTGGCCGAG TTCGAAAAGG CGGTGAGCAT TGCACCCAAC 
CACGCCATCG CCTTCGTCCA GAAGTGCTAT GCCGAGTACC GACTATCCCT CCTGGCCGGC 
GACCAGCGGC GCTTGGAGAG CGTAATGCAC ACCTTCCAAA ATGCCATCGA GCGTTTCCCG 
AGCTGCGTTG AGTGCTATAG TCTAACGGCT CAGGTGTTGG CCGACCAGCA ACAGTTTACC 
CAGGCGGAGG AATACTACAA AAAGGCCATG GTATTGGCGC CAACAAATCC TGCACTTATC 
GTTCACCAGG CCATCATGGT GCTTCAGTGG CGCGGAGACA TTAATTTGGC TGTCCAGCTG 
CTCAACAAGG CGATCGAAGT AGATCCCAAA TGCGAGCTGG CCTACGAGAC ACTCGGAACA 
GTCGAAGTTC AGCGGGCGCA ACTCACACGG GCCGTCGAGC TTTTTGAAAA GGCACTGCTT 
TATGCCAAGA GTCAGGCGGA GTTGGTGCAC GTTTACTCGC TGCGCAACGC GGCAGTGGCC 
CAAATCAACG TCACCAAAAA GCTGGGTATC GACATGAACT CAGTGTCGGC AATGGCCCAA 
TCCGGATTGA TGGCCCAGGG CGTTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609536.1
CDS	181..1950
Translation	MAMTLNIGSMKLTKWQLALILGTPLAIGLGTYAVKSWTAASKELDGEKKRPKAKIEKQAISLDGTAPDQELERNQKSAELGEKLSPLKEANNYKTEGNNCYRNGKYDEAIKFYDKAIDKCPKEHRTDMAIFYQNRAASYEMLKKWSNVKEDCTASLEFNPRYAKAYYRRARAHEATKDMNECLDDVTATCILEMFQNNQTIMFADRVLKETGRLDAEKGMRNRVPVVPSACFVNTYTRSFIADPLQTMKVPAPKSDAPPKGFLRAHLAFLEEKYEEIIPACTEEIESSEAEAQYKVEALLMRGTFHLLCGSYVESQQDFDAIIANDYADPNLRAYAYIKRAALYIQLDQREKGIADFAEAERLNPENPDVYHQRAQILLLLEQIEPALAEFEKAVSIAPNHAIAFVQKCYAEYRLSLLAGDQRRLESVMHTFQNAIERFPSCVECYSLTAQVLADQQQFTQAEEYYKKAMVLAPTNPALIVHQAIMVLQWRGDINLAVQLLNKAIEVDPKCELAYETLGTVEVQRAQLTRAVELFEKALLYAKSQAELVHVYSLRNAAVAQINVTKKLGIDMNSVSAMAQSGLMAQGVA

Target ORF information:

RefSeq Version	NM_135692.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster translocase of outer membrane 70, transcript variant A (Tom70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135692.2

ORF Insert Sequence:

ATGGCGATGA CTCTGAACAT TGGCTCGATG AAGCTGACAA AGTGGCAGCT GGCGCTCATC 
CTTGGAACTC CCCTGGCCAT TGGACTGGGC ACCTATGCGG TGAAGAGCTG GACGGCTGCG 
TCCAAGGAGT TGGACGGGGA GAAGAAGAGA CCAAAGGCCA AGATCGAGAA GCAGGCCATC 
TCCCTGGACG GCACCGCACC GGACCAGGAG CTAGAGCGCA ATCAAAAGTC CGCTGAGCTG 
GGCGAGAAGC TGTCGCCTCT TAAGGAGGCC AACAACTACA AGACGGAGGG TAACAACTGC 
TACAGGAATG GGAAGTACGA TGAGGCCATT AAATTCTATG ACAAGGCCAT CGACAAGTGT 
CCCAAGGAGC ACCGCACTGA CATGGCCATC TTCTACCAGA ACCGCGCCGC CTCTTACGAG 
ATGCTCAAGA AGTGGTCCAA CGTCAAGGAG GACTGCACAG CCTCGCTGGA GTTCAATCCT 
CGTTACGCCA AGGCATATTA TCGTCGTGCC CGTGCTCACG AAGCCACCAA GGATATGAAC 
GAGTGCTTGG ACGATGTCAC AGCCACCTGT ATCCTGGAGA TGTTCCAAAA TAATCAAACT 
ATCATGTTCG CTGACCGTGT GCTCAAGGAG ACCGGCCGAC TGGACGCCGA GAAGGGTATG 
CGCAATCGGG TGCCAGTCGT GCCGTCGGCT TGCTTCGTAA ACACCTACAC GCGTTCCTTC 
ATCGCGGATC CGCTGCAGAC CATGAAAGTC CCAGCCCCTA AATCCGATGC ACCCCCAAAG 
GGTTTCCTGC GCGCCCACCT CGCTTTCCTC GAGGAGAAGT ACGAGGAAAT TATTCCCGCC 
TGCACCGAAG AGATCGAATC CTCCGAGGCC GAAGCACAGT ACAAAGTGGA GGCTCTGCTT 
ATGCGGGGAA CTTTCCATCT GCTGTGCGGC TCCTATGTAG AAAGCCAGCA GGACTTTGAT 
GCTATTATAG CTAATGACTA TGCTGATCCC AACTTGCGTG CGTATGCCTA CATAAAGCGG 
GCAGCTCTCT ACATCCAGCT GGATCAGCGC GAAAAGGGTA TAGCCGATTT TGCTGAGGCG 
GAACGCCTGA ACCCAGAGAA CCCAGATGTG TACCACCAGC GCGCCCAGAT ACTTCTGCTG 
CTGGAGCAGA TAGAGCCCGC CTTGGCCGAG TTCGAAAAGG CGGTGAGCAT TGCACCCAAC 
CACGCCATCG CCTTCGTCCA GAAGTGCTAT GCCGAGTACC GACTATCCCT CCTGGCCGGC 
GACCAGCGGC GCTTGGAGAG CGTAATGCAC ACCTTCCAAA ATGCCATCGA GCGTTTCCCG 
AGCTGCGTTG AGTGCTATAG TCTAACGGCT CAGGTGTTGG CCGACCAGCA ACAGTTTACC 
CAGGCGGAGG AATACTACAA AAAGGCCATG GTATTGGCGC CAACAAATCC TGCACTTATC 
GTTCACCAGG CCATCATGGT GCTTCAGTGG CGCGGAGACA TTAATTTGGC TGTCCAGCTG 
CTCAACAAGG CGATCGAAGT AGATCCCAAA TGCGAGCTGG CCTACGAGAC ACTCGGAACA 
GTCGAAGTTC AGCGGGCGCA ACTCACACGG GCCGTCGAGC TTTTTGAAAA GGCACTGCTT 
TATGCCAAGA GTCAGGCGGA GTTGGTGCAC GTTTACTCGC TGCGCAACGC GGCAGTGGCC 
CAAATCAACG TCACCAAAAA GCTGGGTATC GACATGAACT CAGTGTCGGC AATGGCCCAA 
TCCGGATTGA TGGCCCAGGG CGTTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tom70 ( NM_164986.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723712.1 Drosophila melanogaster translocase of outer membrane 70, transcript variant C (Tom70), mRNA.

Price & Availability↑

CloneID	OFa14492
Clone ID Related Accession (Same CDS sequence)	NM_164985.2 , NM_135692.2 , NM_164986.2
Accession Version	NM_164986.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	translocase of outer membrane 70, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_164986.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGATGA CTCTGAACAT TGGCTCGATG AAGCTGACAA AGTGGCAGCT GGCGCTCATC 
CTTGGAACTC CCCTGGCCAT TGGACTGGGC ACCTATGCGG TGAAGAGCTG GACGGCTGCG 
TCCAAGGAGT TGGACGGGGA GAAGAAGAGA CCAAAGGCCA AGATCGAGAA GCAGGCCATC 
TCCCTGGACG GCACCGCACC GGACCAGGAG CTAGAGCGCA ATCAAAAGTC CGCTGAGCTG 
GGCGAGAAGC TGTCGCCTCT TAAGGAGGCC AACAACTACA AGACGGAGGG TAACAACTGC 
TACAGGAATG GGAAGTACGA TGAGGCCATT AAATTCTATG ACAAGGCCAT CGACAAGTGT 
CCCAAGGAGC ACCGCACTGA CATGGCCATC TTCTACCAGA ACCGCGCCGC CTCTTACGAG 
ATGCTCAAGA AGTGGTCCAA CGTCAAGGAG GACTGCACAG CCTCGCTGGA GTTCAATCCT 
CGTTACGCCA AGGCATATTA TCGTCGTGCC CGTGCTCACG AAGCCACCAA GGATATGAAC 
GAGTGCTTGG ACGATGTCAC AGCCACCTGT ATCCTGGAGA TGTTCCAAAA TAATCAAACT 
ATCATGTTCG CTGACCGTGT GCTCAAGGAG ACCGGCCGAC TGGACGCCGA GAAGGGTATG 
CGCAATCGGG TGCCAGTCGT GCCGTCGGCT TGCTTCGTAA ACACCTACAC GCGTTCCTTC 
ATCGCGGATC CGCTGCAGAC CATGAAAGTC CCAGCCCCTA AATCCGATGC ACCCCCAAAG 
GGTTTCCTGC GCGCCCACCT CGCTTTCCTC GAGGAGAAGT ACGAGGAAAT TATTCCCGCC 
TGCACCGAAG AGATCGAATC CTCCGAGGCC GAAGCACAGT ACAAAGTGGA GGCTCTGCTT 
ATGCGGGGAA CTTTCCATCT GCTGTGCGGC TCCTATGTAG AAAGCCAGCA GGACTTTGAT 
GCTATTATAG CTAATGACTA TGCTGATCCC AACTTGCGTG CGTATGCCTA CATAAAGCGG 
GCAGCTCTCT ACATCCAGCT GGATCAGCGC GAAAAGGGTA TAGCCGATTT TGCTGAGGCG 
GAACGCCTGA ACCCAGAGAA CCCAGATGTG TACCACCAGC GCGCCCAGAT ACTTCTGCTG 
CTGGAGCAGA TAGAGCCCGC CTTGGCCGAG TTCGAAAAGG CGGTGAGCAT TGCACCCAAC 
CACGCCATCG CCTTCGTCCA GAAGTGCTAT GCCGAGTACC GACTATCCCT CCTGGCCGGC 
GACCAGCGGC GCTTGGAGAG CGTAATGCAC ACCTTCCAAA ATGCCATCGA GCGTTTCCCG 
AGCTGCGTTG AGTGCTATAG TCTAACGGCT CAGGTGTTGG CCGACCAGCA ACAGTTTACC 
CAGGCGGAGG AATACTACAA AAAGGCCATG GTATTGGCGC CAACAAATCC TGCACTTATC 
GTTCACCAGG CCATCATGGT GCTTCAGTGG CGCGGAGACA TTAATTTGGC TGTCCAGCTG 
CTCAACAAGG CGATCGAAGT AGATCCCAAA TGCGAGCTGG CCTACGAGAC ACTCGGAACA 
GTCGAAGTTC AGCGGGCGCA ACTCACACGG GCCGTCGAGC TTTTTGAAAA GGCACTGCTT 
TATGCCAAGA GTCAGGCGGA GTTGGTGCAC GTTTACTCGC TGCGCAACGC GGCAGTGGCC 
CAAATCAACG TCACCAAAAA GCTGGGTATC GACATGAACT CAGTGTCGGC AATGGCCCAA 
TCCGGATTGA TGGCCCAGGG CGTTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723712.1
CDS	260..2029
Translation	MAMTLNIGSMKLTKWQLALILGTPLAIGLGTYAVKSWTAASKELDGEKKRPKAKIEKQAISLDGTAPDQELERNQKSAELGEKLSPLKEANNYKTEGNNCYRNGKYDEAIKFYDKAIDKCPKEHRTDMAIFYQNRAASYEMLKKWSNVKEDCTASLEFNPRYAKAYYRRARAHEATKDMNECLDDVTATCILEMFQNNQTIMFADRVLKETGRLDAEKGMRNRVPVVPSACFVNTYTRSFIADPLQTMKVPAPKSDAPPKGFLRAHLAFLEEKYEEIIPACTEEIESSEAEAQYKVEALLMRGTFHLLCGSYVESQQDFDAIIANDYADPNLRAYAYIKRAALYIQLDQREKGIADFAEAERLNPENPDVYHQRAQILLLLEQIEPALAEFEKAVSIAPNHAIAFVQKCYAEYRLSLLAGDQRRLESVMHTFQNAIERFPSCVECYSLTAQVLADQQQFTQAEEYYKKAMVLAPTNPALIVHQAIMVLQWRGDINLAVQLLNKAIEVDPKCELAYETLGTVEVQRAQLTRAVELFEKALLYAKSQAELVHVYSLRNAAVAQINVTKKLGIDMNSVSAMAQSGLMAQGVA

Target ORF information:

RefSeq Version	NM_164986.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster translocase of outer membrane 70, transcript variant C (Tom70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164986.2

ORF Insert Sequence:

ATGGCGATGA CTCTGAACAT TGGCTCGATG AAGCTGACAA AGTGGCAGCT GGCGCTCATC 
CTTGGAACTC CCCTGGCCAT TGGACTGGGC ACCTATGCGG TGAAGAGCTG GACGGCTGCG 
TCCAAGGAGT TGGACGGGGA GAAGAAGAGA CCAAAGGCCA AGATCGAGAA GCAGGCCATC 
TCCCTGGACG GCACCGCACC GGACCAGGAG CTAGAGCGCA ATCAAAAGTC CGCTGAGCTG 
GGCGAGAAGC TGTCGCCTCT TAAGGAGGCC AACAACTACA AGACGGAGGG TAACAACTGC 
TACAGGAATG GGAAGTACGA TGAGGCCATT AAATTCTATG ACAAGGCCAT CGACAAGTGT 
CCCAAGGAGC ACCGCACTGA CATGGCCATC TTCTACCAGA ACCGCGCCGC CTCTTACGAG 
ATGCTCAAGA AGTGGTCCAA CGTCAAGGAG GACTGCACAG CCTCGCTGGA GTTCAATCCT 
CGTTACGCCA AGGCATATTA TCGTCGTGCC CGTGCTCACG AAGCCACCAA GGATATGAAC 
GAGTGCTTGG ACGATGTCAC AGCCACCTGT ATCCTGGAGA TGTTCCAAAA TAATCAAACT 
ATCATGTTCG CTGACCGTGT GCTCAAGGAG ACCGGCCGAC TGGACGCCGA GAAGGGTATG 
CGCAATCGGG TGCCAGTCGT GCCGTCGGCT TGCTTCGTAA ACACCTACAC GCGTTCCTTC 
ATCGCGGATC CGCTGCAGAC CATGAAAGTC CCAGCCCCTA AATCCGATGC ACCCCCAAAG 
GGTTTCCTGC GCGCCCACCT CGCTTTCCTC GAGGAGAAGT ACGAGGAAAT TATTCCCGCC 
TGCACCGAAG AGATCGAATC CTCCGAGGCC GAAGCACAGT ACAAAGTGGA GGCTCTGCTT 
ATGCGGGGAA CTTTCCATCT GCTGTGCGGC TCCTATGTAG AAAGCCAGCA GGACTTTGAT 
GCTATTATAG CTAATGACTA TGCTGATCCC AACTTGCGTG CGTATGCCTA CATAAAGCGG 
GCAGCTCTCT ACATCCAGCT GGATCAGCGC GAAAAGGGTA TAGCCGATTT TGCTGAGGCG 
GAACGCCTGA ACCCAGAGAA CCCAGATGTG TACCACCAGC GCGCCCAGAT ACTTCTGCTG 
CTGGAGCAGA TAGAGCCCGC CTTGGCCGAG TTCGAAAAGG CGGTGAGCAT TGCACCCAAC 
CACGCCATCG CCTTCGTCCA GAAGTGCTAT GCCGAGTACC GACTATCCCT CCTGGCCGGC 
GACCAGCGGC GCTTGGAGAG CGTAATGCAC ACCTTCCAAA ATGCCATCGA GCGTTTCCCG 
AGCTGCGTTG AGTGCTATAG TCTAACGGCT CAGGTGTTGG CCGACCAGCA ACAGTTTACC 
CAGGCGGAGG AATACTACAA AAAGGCCATG GTATTGGCGC CAACAAATCC TGCACTTATC 
GTTCACCAGG CCATCATGGT GCTTCAGTGG CGCGGAGACA TTAATTTGGC TGTCCAGCTG 
CTCAACAAGG CGATCGAAGT AGATCCCAAA TGCGAGCTGG CCTACGAGAC ACTCGGAACA 
GTCGAAGTTC AGCGGGCGCA ACTCACACGG GCCGTCGAGC TTTTTGAAAA GGCACTGCTT 
TATGCCAAGA GTCAGGCGGA GTTGGTGCAC GTTTACTCGC TGCGCAACGC GGCAGTGGCC 
CAAATCAACG TCACCAAAAA GCTGGGTATC GACATGAACT CAGTGTCGGC AATGGCCCAA 
TCCGGATTGA TGGCCCAGGG CGTTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tom70 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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