zuc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	zuc
Entrez Gene ID	34609
Full Name	zucchini
Synonyms	CG12314,Dmel\CG12314,MitoPLD,ZUC,Zuc
General protein information	Preferred Names zucchini Names CG12314 gene product from transcript CG12314-RA CG12314-PA zuc-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 33B5-33B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_135686.3	NP_609530.1	zucchini

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	zuc	NP_609530.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006233	XP_316301.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A heterologous RNA that lacks complementary piRNAs is processed into piRNAs upon recruitment of several piRNA pathway factors. This processing requires Zuc and the helicase Armitage (Armi).
Title: Zucchini-dependent piRNA processing is triggered by recruitment to the cytoplasmic processing machinery.

these data uncover an evolutionarily conserved piRNA biogenesis mechanism in which Zucchini plays a central role in defining piRNA 5' and 3' ends.
Title: Noncoding RNA. piRNA-guided slicing specifies transcripts for Zucchini-dependent, phased piRNA biogenesis.

The active site is situated on the dimer interface at the bottom of a narrow groove that can likely accommodate single-stranded nucleic acid substrates.
Title: Crystal structure of the primary piRNA biogenesis factor Zucchini reveals similarity to the bacterial PLD endonuclease Nuc.

Zucchini (Zuc), a mitochondrial phospholipase D (PLD) superfamily member, is an endoribonuclease essential for primary piRNA biogenesis; crystal structure of Drosophila melanogaster Zuc (DmZuc) at 1.75 A resolution
Title: Structure and function of Zucchini endoribonuclease in piRNA biogenesis.

preliminary X-ray crystallographic analysis of Zucchini from Drosophila melanogaster
Title: Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Zucchini from Drosophila melanogaster.

The following zuc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the zuc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14485	NM_135686.3 Latest version!	Drosophila melanogaster zucchini (zuc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

zuc ( NM_135686.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609530.1 Drosophila melanogaster zucchini (zuc), mRNA.

Price & Availability↑

CloneID	OFa14485
Clone ID Related Accession (Same CDS sequence)	NM_135686.3
Accession Version	NM_135686.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	zucchini
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135686.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGATTA CCCAAATAAT TATGAAACAA ATTCGAGACT ATCCCATTGT GTCCACAATA 
AGCATTGCCG TCAGCACTGT CCTGGCCTCA GAGGTGATTT GGAAGCTGGT GCAGTGCTCG 
CGCAGCAAGC GAGAGAAGGC AAGCCGCGTG CACGAAGTGA TCATCTTCAA TGAACTGGGC 
GAGATCTGTG CGGCGGTGCA TATGCGCAAC AGCAGCATGG GATCCCAGAA ACCACAAGTG 
TCGCCCTGCT GCAACACGCA TTGTTCGCTG CGGAATGTGG CCAAGATCGT AGAGCAGATC 
GATCGGGCTG TATACTCCAT TGACCTGGCC ATATACACCT TCACCTCGCT TTTTTTGGCG 
GATTCAATAA AGCGGGCCCT GCAGCGCGGC GTGATTATCC GGATCATCAG CGACGGGGAG 
ATGGTCTACT CCAAAGGTTC TCAGATCAGT ATGCTGGCAC AATTGGGAGT TCCAGTTCGC 
GTCCCCATTA CCACGAACTT GATGCACAAC AAATTCTGCA TAATCGACGG CTTCGAGCGC 
GTCGAGGAGA TTCGTCTGCT GAGGAAGCTC AAGTTTATGC GTCCGTGCTA TAGCATCGTG 
ATCAGCGGCT CCGTAAACTG GACGGCTCTT GGACTGGGAG GCAATTGGGA GAACTGCATT 
ATCACAGCTG ACGACAAACT CACAGCCACG TTTCAGGCGG AATTCCAACG AATGTGGCGG 
GCTTTTGCGA AGACCGAGGG GAGCCAAATC CAGCTCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609530.1
CDS	59..820
Translation	MLITQIIMKQIRDYPIVSTISIAVSTVLASEVIWKLVQCSRSKREKASRVHEVIIFNELGEICAAVHMRNSSMGSQKPQVSPCCNTHCSLRNVAKIVEQIDRAVYSIDLAIYTFTSLFLADSIKRALQRGVIIRIISDGEMVYSKGSQISMLAQLGVPVRVPITTNLMHNKFCIIDGFERVEEIRLLRKLKFMRPCYSIVISGSVNWTALGLGGNWENCIITADDKLTATFQAEFQRMWRAFAKTEGSQIQLK

Target ORF information:

RefSeq Version	NM_135686.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster zucchini (zuc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135686.3

ORF Insert Sequence:

ATGTTGATTA CCCAAATAAT TATGAAACAA ATTCGAGACT ATCCCATTGT GTCCACAATA 
AGCATTGCCG TCAGCACTGT CCTGGCCTCA GAGGTGATTT GGAAGCTGGT GCAGTGCTCG 
CGCAGCAAGC GAGAGAAGGC AAGCCGCGTG CACGAAGTGA TCATCTTCAA TGAACTGGGC 
GAGATCTGTG CGGCGGTGCA TATGCGCAAC AGCAGCATGG GATCCCAGAA ACCACAAGTG 
TCGCCCTGCT GCAACACGCA TTGTTCGCTG CGGAATGTGG CCAAGATCGT AGAGCAGATC 
GATCGGGCTG TATACTCCAT TGACCTGGCC ATATACACCT TCACCTCGCT TTTTTTGGCG 
GATTCAATAA AGCGGGCCCT GCAGCGCGGC GTGATTATCC GGATCATCAG CGACGGGGAG 
ATGGTCTACT CCAAAGGTTC TCAGATCAGT ATGCTGGCAC AATTGGGAGT TCCAGTTCGC 
GTCCCCATTA CCACGAACTT GATGCACAAC AAATTCTGCA TAATCGACGG CTTCGAGCGC 
GTCGAGGAGA TTCGTCTGCT GAGGAAGCTC AAGTTTATGC GTCCGTGCTA TAGCATCGTG 
ATCAGCGGCT CCGTAAACTG GACGGCTCTT GGACTGGGAG GCAATTGGGA GAACTGCATT 
ATCACAGCTG ACGACAAACT CACAGCCACG TTTCAGGCGG AATTCCAACG AATGTGGCGG 
GCTTTTGCGA AGACCGAGGG GAGCCAAATC CAGCTCAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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zuc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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