l(2)gd1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	l(2)gd1
Entrez Gene ID	34543
Full Name	lethal (2) giant discs 1
Synonyms	CG4713,Dmel\CG4713,SH495,gd,l(2)SH0495,l(2)SH2 0495,l(2)gd,l(2)giant disc,l[[2]]gd,lgd
General protein information	Preferred Names lethal (2) giant discs 1 Names CG4713 gene product from transcript CG4713-RB CG4713-PA CG4713-PB l(2)gd1-PA l(2)gd1-PB leathal giant disc lethal (2) SH0495 lethal (2) giant discs lethal giant discs
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32D1-32D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_135644.4	NP_609488.1	lethal (2) giant discs 1, isoform A
NM_001259047.2	NP_001245976.1	lethal (2) giant discs 1, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CC2D1A	XP_524131.4
Xenopus tropicalis (tropical clawed frog)	cc2d1a	NP_001120385.1
Homo sapiens (human)	CC2D1A	NP_060191.3
Bos taurus (cattle)	CC2D1A	NP_001092424.1
Rattus norvegicus (Norway rat)	Cc2d1a	NP_001013891.1
Drosophila melanogaster (fruit fly)	l(2)gd1	NP_001245976.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010539	XP_314506.4
Caenorhabditis elegans (roundworm)	CELE_Y37H9A.3	NP_493412.2
	CC2D1A	XP_001111234.2
Canis lupus familiaris (dog)	CC2D1A	XP_542027.3
Mus musculus (house mouse)	Cc2d1a	NP_666082.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

During oogenesis lgd loss of function causes ectopic activation of the Drosophila BMP signalling pathway.
Title: Lgd regulates the activity of the BMP/Dpp signalling pathway during Drosophila oogenesis.

lgd cells have a defect that delays degradation of transmembrane proteins, which are residents of the plasma membrane. Furthermore, our results show that the activation of Notch in lgd cells occurs in the lysosome.
Title: Activation of Notch in lgd mutant cells requires the fusion of late endosomes with the lysosome.

genetic studies indicate that the activation of Notch in ESCRT and lgd mutant cells occurs in a different manner and that the activity of Shrub and other ESCRT components are required for the activation of Notch in lgd mutant cells
Title: The tumour suppressor Lethal (2) giant discs is required for the function of the ESCRT-III component Shrub/CHMP4.

The following l(2)gd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the l(2)gd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14448	NM_001259047.2 Latest version!	Drosophila melanogaster lethal (2) giant discs 1 (l(2)gd1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16655	NM_135644.4 Latest version!	Drosophila melanogaster lethal (2) giant discs 1 (l(2)gd1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

l(2)gd1 ( NM_001259047.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245976.1 Drosophila melanogaster lethal (2) giant discs 1 (l(2)gd1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14448
Clone ID Related Accession (Same CDS sequence)	NM_001259047.2
Accession Version	NM_001259047.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (2) giant discs 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001259047.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCTCCA GAAAGAAGCC AGAGCCAGCG AAAAGAAGGC AACACGATTT ATCGCAGTTC 
GGTCTTACGG AAATCCCAGA TGATTTTGAT CCCAGCGCTG GATATGGTGA GGACGATGGG 
GGTGACAGTG ATTTGGAGGC AGAGCTTGCG GCCATAACCG GCGGAGAAGG AGCCAAGCCA 
AAGCCCAAGC CCAAAGCAAA GCTGTTACCC GCCAGTGATT TGGACAAAAT GATAGCCGAC 
AGCTTGCGTG ATGTCAGCGA CGATGATGAT GATGACAATT TGGAAAGCGA TCCGGATCTT 
CTTGGCGAGC TGAGCGGAAT CGGTGGTCTA GAGGAGGCAG AGGAAGAGGA GCCTGTCGCA 
CAACCGCCAG CTGCCTCTGA GGAACCCGTC CAGACCTTTC TGCCCACGAC GACTGTGGAC 
ACATTAAGCA TTATCAAACA GCGCCTGGAA ATGTACAAGC AGGCCGAGGC CAATGCCAAG 
ACTGCCGGCG ATTCTGGCAA AGCAAGGCGA TTTGGAAGAG GCCTTAAGAC TCTGAAAGAT 
CTGCACAGGC AGGCAGCGGC TGGCAAGTCC ATTAACGTGG ATGACATTCC ACCTGAAGTC 
AGTGTAAAGC CAATTGGCGG GCAGGCTCCT CCAGTGCCTG CAGAGGAGTC ACCAGCTCCT 
TCCACACCTG CTTCCCCTCC GCCTGTGCCT AGTCGTGCAG CTCCAGATCC ACCAACCCCC 
GGCACCCCCG TGGAGCCCAC TACATCTGTG GCTCCCACAT CTCCCCCTAA TCCTCTTGTG 
ACGCAAATGC GCAGCCGCCA GACGGACTAC AAAGCTGCTG CACTGCAATC TAAGCGCAGT 
GGTGACATAT CTACTGCCCT TCAGTTTCTC AAAGTGGTCA AGCAATTTGA CGTAGTTATA 
AAAATGTGTG AAGACGGACA GGAAGTTGAT TTGAGTGACA TGCCCCCTCC ACCAGCTGAG 
TTTCTTGAAT TCCTTAAAAA AATGCAAGAA GAAGCAGCCG CAGAAGCTGT CGCAGAACCT 
ACTGCTGCCC CAGAACCCAC ACCTGTTGCT CCTGCTCCTG TTCTAGCAGC TGCAACAAAT 
ATGTTGGAGG CTCTGCAGCA ACGTTTAGAG AAATATCAAT CTGTAGAAGC GGCAGCCAAG 
GCAGAAAACA ATAGCGGAAA AGCAAGACGC TTTGGAAGGA TTGTGAAGCA ATACGAAGAT 
GCCATAAAGT TGTACAAGGC AGGCAAACCA GTGCCTTACG ATGAGCTGCC TGTGCCACCA 
GGTTTTGGAC CGCTGCCTAC GGCTGATGCT GCTCCTGTCG CGCCGACACC GTCGCTGCCC 
ACTTCGCCTA CATCTCCACC GCCAACAGCA AGTACTTCCG CAGGAGGAAC ACCCTCCAGT 
TCCAGTGCGA CGACACCTAC AGCTCCTCGA AAAGCACCTT CACCTCCTAA GCCCAAAGAG 
TTAACAACAC GCACGTCTGG CAATCAGCAG AAGAATAATA TTGCCGAGCA ACAAATGAAA 
CTGCTCCTCG AGCGCCAAAA GGAGTTTAAG CTAGCTGCTA TAGAGGCCAA AAAAGCGGGA 
GAAATAGATC AGGCGAAGGA GTACCTCAAG ATCTTCAAGG GATTCGATTC CCTTCTTAAT 
GCAGCGAGTA GTGGATTGCC AGTGGACCTC AGCACTCTTC CCGTCCCGCC TTCACAAAGG 
GATAATCTAG AAGCCTCCTT TGCCATTGTA TCTGCTGAGG AATGCGATCC GACAGATGAT 
ATCTGTGAAA TTGGTGTTCG CATGGAGGAG CAGCTGGCAA AACAACTGAT GATGTGCAAA 
AATACGCGAG ATCATCACAA AGCTATGGGA GATGTAGCAG GCATGAATCG GTTTGAAAAT 
TTAGCCTTAA CTGTGCAAAA GGATTTGGAT TTGGTGCGAT ACTCGAAACG AAAGAATGAA 
CCACTGCCAA AGTTTCACTA TGAAAAGCGT AGCTTCAACA TTGTGCATTG CAACACAGAT 
CTCACAGACA GTGAGCTTGA AATTGTTGTG GTCCGGGGAA TCAGCTACAA TGTGGCCAAT 
CCCAAGGACG TGGATACTTA TGTGCGCGTA GAGTTTCCCC TGCTCAATGA TGAATCTTTC 
AAGACTAAAA CCAATGTTAT CAGAGACACC AGCAGTCCTG ACTACGATGA ACGCTTCAAG 
GTTGACATCC AGCGGACCAA TCGTCAGTTC CAGAGAATCT TTAAGCGGCA TGGCGTCAAG 
TTTGAAATAT ACTCTAGAGG CTGCAGTATA GATTGTTGTG GACTAAGTCG TAAACTGCCC 
CTGTGTTGTT TCAGAGGTTT TCTGCGATCG GATACGTTGA TCGGAACCGT AAATGTCAAG 
CTGCAGCCAT TGGAGACCAA GTGCGAGATA CACGACACAT ATGATCTAAT GGATGGTCGC 
AAGCAAGTAG GTGGAAAATT GGAAGTCAAG ATACGCGTTC GCAATCCTAT TCTCACCAAG 
CAAATGGAGC ACATCACCGA AAAGTGGTTG GTCTTGGATG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245976.1
CDS	123..2627
Translation	MFSRKKPEPAKRRQHDLSQFGLTEIPDDFDPSAGYGEDDGGDSDLEAELAAITGGEGAKPKPKPKAKLLPASDLDKMIADSLRDVSDDDDDDNLESDPDLLGELSGIGGLEEAEEEEPVAQPPAASEEPVQTFLPTTTVDTLSIIKQRLEMYKQAEANAKTAGDSGKARRFGRGLKTLKDLHRQAAAGKSINVDDIPPEVSVKPIGGQAPPVPAEESPAPSTPASPPPVPSRAAPDPPTPGTPVEPTTSVAPTSPPNPLVTQMRSRQTDYKAAALQSKRSGDISTALQFLKVVKQFDVVIKMCEDGQEVDLSDMPPPPAEFLEFLKKMQEEAAAEAVAEPTAAPEPTPVAPAPVLAAATNMLEALQQRLEKYQSVEAAAKAENNSGKARRFGRIVKQYEDAIKLYKAGKPVPYDELPVPPGFGPLPTADAAPVAPTPSLPTSPTSPPPTASTSAGGTPSSSSATTPTAPRKAPSPPKPKELTTRTSGNQQKNNIAEQQMKLLLERQKEFKLAAIEAKKAGEIDQAKEYLKIFKGFDSLLNAASSGLPVDLSTLPVPPSQRDNLEASFAIVSAEECDPTDDICEIGVRMEEQLAKQLMMCKNTRDHHKAMGDVAGMNRFENLALTVQKDLDLVRYSKRKNEPLPKFHYEKRSFNIVHCNTDLTDSELEIVVVRGISYNVANPKDVDTYVRVEFPLLNDESFKTKTNVIRDTSSPDYDERFKVDIQRTNRQFQRIFKRHGVKFEIYSRGCSIDCCGLSRKLPLCCFRGFLRSDTLIGTVNVKLQPLETKCEIHDTYDLMDGRKQVGGKLEVKIRVRNPILTKQMEHITEKWLVLDA

Target ORF information:

RefSeq Version	NM_001259047.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (2) giant discs 1 (l(2)gd1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259047.2

ORF Insert Sequence:

ATGTTCTCCA GAAAGAAGCC AGAGCCAGCG AAAAGAAGGC AACACGATTT ATCGCAGTTC 
GGTCTTACGG AAATCCCAGA TGATTTTGAT CCCAGCGCTG GATATGGTGA GGACGATGGG 
GGTGACAGTG ATTTGGAGGC AGAGCTTGCG GCCATAACCG GCGGAGAAGG AGCCAAGCCA 
AAGCCCAAGC CCAAAGCAAA GCTGTTACCC GCCAGTGATT TGGACAAAAT GATAGCCGAC 
AGCTTGCGTG ATGTCAGCGA CGATGATGAT GATGACAATT TGGAAAGCGA TCCGGATCTT 
CTTGGCGAGC TGAGCGGAAT CGGTGGTCTA GAGGAGGCAG AGGAAGAGGA GCCTGTCGCA 
CAACCGCCAG CTGCCTCTGA GGAACCCGTC CAGACCTTTC TGCCCACGAC GACTGTGGAC 
ACATTAAGCA TTATCAAACA GCGCCTGGAA ATGTACAAGC AGGCCGAGGC CAATGCCAAG 
ACTGCCGGCG ATTCTGGCAA AGCAAGGCGA TTTGGAAGAG GCCTTAAGAC TCTGAAAGAT 
CTGCACAGGC AGGCAGCGGC TGGCAAGTCC ATTAACGTGG ATGACATTCC ACCTGAAGTC 
AGTGTAAAGC CAATTGGCGG GCAGGCTCCT CCAGTGCCTG CAGAGGAGTC ACCAGCTCCT 
TCCACACCTG CTTCCCCTCC GCCTGTGCCT AGTCGTGCAG CTCCAGATCC ACCAACCCCC 
GGCACCCCCG TGGAGCCCAC TACATCTGTG GCTCCCACAT CTCCCCCTAA TCCTCTTGTG 
ACGCAAATGC GCAGCCGCCA GACGGACTAC AAAGCTGCTG CACTGCAATC TAAGCGCAGT 
GGTGACATAT CTACTGCCCT TCAGTTTCTC AAAGTGGTCA AGCAATTTGA CGTAGTTATA 
AAAATGTGTG AAGACGGACA GGAAGTTGAT TTGAGTGACA TGCCCCCTCC ACCAGCTGAG 
TTTCTTGAAT TCCTTAAAAA AATGCAAGAA GAAGCAGCCG CAGAAGCTGT CGCAGAACCT 
ACTGCTGCCC CAGAACCCAC ACCTGTTGCT CCTGCTCCTG TTCTAGCAGC TGCAACAAAT 
ATGTTGGAGG CTCTGCAGCA ACGTTTAGAG AAATATCAAT CTGTAGAAGC GGCAGCCAAG 
GCAGAAAACA ATAGCGGAAA AGCAAGACGC TTTGGAAGGA TTGTGAAGCA ATACGAAGAT 
GCCATAAAGT TGTACAAGGC AGGCAAACCA GTGCCTTACG ATGAGCTGCC TGTGCCACCA 
GGTTTTGGAC CGCTGCCTAC GGCTGATGCT GCTCCTGTCG CGCCGACACC GTCGCTGCCC 
ACTTCGCCTA CATCTCCACC GCCAACAGCA AGTACTTCCG CAGGAGGAAC ACCCTCCAGT 
TCCAGTGCGA CGACACCTAC AGCTCCTCGA AAAGCACCTT CACCTCCTAA GCCCAAAGAG 
TTAACAACAC GCACGTCTGG CAATCAGCAG AAGAATAATA TTGCCGAGCA ACAAATGAAA 
CTGCTCCTCG AGCGCCAAAA GGAGTTTAAG CTAGCTGCTA TAGAGGCCAA AAAAGCGGGA 
GAAATAGATC AGGCGAAGGA GTACCTCAAG ATCTTCAAGG GATTCGATTC CCTTCTTAAT 
GCAGCGAGTA GTGGATTGCC AGTGGACCTC AGCACTCTTC CCGTCCCGCC TTCACAAAGG 
GATAATCTAG AAGCCTCCTT TGCCATTGTA TCTGCTGAGG AATGCGATCC GACAGATGAT 
ATCTGTGAAA TTGGTGTTCG CATGGAGGAG CAGCTGGCAA AACAACTGAT GATGTGCAAA 
AATACGCGAG ATCATCACAA AGCTATGGGA GATGTAGCAG GCATGAATCG GTTTGAAAAT 
TTAGCCTTAA CTGTGCAAAA GGATTTGGAT TTGGTGCGAT ACTCGAAACG AAAGAATGAA 
CCACTGCCAA AGTTTCACTA TGAAAAGCGT AGCTTCAACA TTGTGCATTG CAACACAGAT 
CTCACAGACA GTGAGCTTGA AATTGTTGTG GTCCGGGGAA TCAGCTACAA TGTGGCCAAT 
CCCAAGGACG TGGATACTTA TGTGCGCGTA GAGTTTCCCC TGCTCAATGA TGAATCTTTC 
AAGACTAAAA CCAATGTTAT CAGAGACACC AGCAGTCCTG ACTACGATGA ACGCTTCAAG 
GTTGACATCC AGCGGACCAA TCGTCAGTTC CAGAGAATCT TTAAGCGGCA TGGCGTCAAG 
TTTGAAATAT ACTCTAGAGG CTGCAGTATA GATTGTTGTG GACTAAGTCG TAAACTGCCC 
CTGTGTTGTT TCAGAGGTTT TCTGCGATCG GATACGTTGA TCGGAACCGT AAATGTCAAG 
CTGCAGCCAT TGGAGACCAA GTGCGAGATA CACGACACAT ATGATCTAAT GGATGGTCGC 
AAGCAAGTAG GTGGAAAATT GGAAGTCAAG ATACGCGTTC GCAATCCTAT TCTCACCAAG 
CAAATGGAGC ACATCACCGA AAAGTGGTTG GTCTTGGATG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

l(2)gd1 ( NM_135644.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609488.1 Drosophila melanogaster lethal (2) giant discs 1 (l(2)gd1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16655
Clone ID Related Accession (Same CDS sequence)	NM_135644.4
Accession Version	NM_135644.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (2) giant discs 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135644.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCTCCA GAAAGAAGCC AGAGCCAGCG AAAAGAAGGC AACACGATTT ATCGCAGTTC 
GGTCTTACGG AAATCCCAGA TGATTTTGAT CCCAGCGCTG GATATGGTGA GGACGATGGG 
GGTGACAGTG ATTTGGAGGC AGAGCTTGCG GCCATAACCG GCGGAGAAGG AGCCAAGCCA 
AAGCCCAAGC CCAAAGCAAA GCTGTTACCC GCCAGTGATT TGGACAAAAT GATAGCCGAC 
AGCTTGCGTG ATGTCAGCGA CGATGATGAT GATGACAATT TGGAAAGCGA TCCGGATCTT 
CTTGGCGAGC TGAGCGGAAT CGGTGGTCTA GAGGAGGCAG AGGAAGAGGA GCCTGTCGCA 
CAACCGCCAG CTGCCTCTGA GGAACCCGTC CAGACCTTTC TGCCCACGAC GACTGTGGAC 
ACATTAAGCA TTATCAAACA GCGCCTGGAA ATGTACAAGC AGGCCGAGGC CAATGCCAAG 
ACTGCCGGCG ATTCTGGCAA AGCAAGGCGA TTTGGAAGAG GCCTTAAGAC TCTGAAAGAT 
CTGCACAGGC AGGCAGCGGC TGGCAAGTCC ATTAACGTGG ATGACATTCC ACCTGAAGTC 
AGTGTAAAGC CAATTGGCGG GCAGGCTCCT CCAGTGCCTG CAGAGGAGTC ACCAGCTCCT 
TCCACACCTG CTTCCCCTCC GCCTGTGCCT AGTCGTGCAG CTCCAGATCC ACCAACCCCC 
GGCACCCCCG TGGAGCCCAC TACATCTGTG GCTCCCACAT CTCCCCCTAA TCCTCTTGTG 
ACGCAAATGC GCAGCCGCCA GACGGACTAC AAAGCTGCTG CACTGCAATC TAAGCGCAGT 
GGTGACATAT CTACTGCCCT TCAGTTTCTC AAAGTGGTCA AGCAATTTGA CGTAGTTATA 
AAAATGTGTG AAGACGGACA GGAAGTTGAT TTGAGTGACA TGCCCCCTCC ACCAGCTGAG 
TTTCTTGAAT TCCTTAAAAA AATGCAAGAA GAAGCAGCCG CAGAAGCTGT CGCAGAACCT 
ACTGCTGCCC CAGAACCCAC ACCTGTTGCT CCTGCTCCTG TTCTAGCAGC TGCAACAAAT 
ATGTTGGAGG CTCTGCAGCA ACGTTTAGAG AAATATCAAT CTGTAGAAGC GGCAGCCAAG 
GCAGAAAACA ATAGCGGAAA AGCAAGACGC TTTGGAAGGA TTGTGAAGCA ATACGAAGAT 
GCCATAAAGT TGTACAAGGC AGGCAAACCA GTGCCTTACG ATGAGCTGCC TGTGCCACCA 
GGTTTTGGAC CGCTGCCTAC GGCTGATGCT GCTCCTGTCG CGCCGACACC GTCGCTGCCC 
ACTTCGCCTA CATCTCCACC GCCAACAGCA AGTACTTCCG CAGGAGGAAC ACCCTCCAGT 
TCCAGTGCGA CGACACCTAC AGCTCCTCGA AAAGCACCTT CACCTCCTAA GCCCAAAGAG 
TTAACAACAC GCACGTCTGG CAATCAGCAG AAGAATAATA TTGCCGAGCA ACAAATGAAA 
CTGCTCCTCG AGCGCCAAAA GGAGTTTAAG CTAGCTGCTA TAGAGGCCAA AAAAGCGGGA 
GAAATAGATC AGGCGAAGGA GTACCTCAAG ATCTTCAAGG GATTCGATTC CCTTCTTAAT 
GCAGCGAGTA GTGGATTGCC AGTGGACCTC AGCACTCTTC CCGTCCCGCC TTCACAAAGG 
GATAATCTAG AAGCCTCCTT TGCCATTGTA TCTGCTGAGG AATGCGATCC GACAGATGAT 
ATCTGTGAAA TTGGTGTTCG CATGGAGGAG CAGCTGGCAA AACAACTGAT GATGTGCAAA 
AATACGCGAG ATCATCACAA AGCTATGGGA GATGTAGCAG GCATGAATCG GTTTGAAAAT 
TTAGCCTTAA CTGTGCAAAA GGATTTGGAT TTGGTGCGAT ACTCGAAACG AAAGAATGAA 
CCACTGCCAA AGTTTCACTA TGAAAAGCGT AGCTTCAACA TTGTGCATTG CAACACAGAT 
CTCACAGACA GTGAGCTTGA AATTGTTGTG GTCCGGGGAA TCAGCTACAA TGTGGCCAAT 
CCCAAGGACG TGGATACTTA TGTGCGCGTA GAGTTTCCCC TGCTCAATGA TGAATCTTTC 
AAGACTAAAA CCAATGTTAT CAGAGACACC AGCAGTCCTG ACTACGATGA ACGCTTCAAG 
GTTGACATCC AGCGGACCAA TCGTCAGTTC CAGAGAATCT TTAAGCGGCA TGGCGTCAAG 
TTTGAAATAT ACTCTAGAGG AGGTTTTCTG CGATCGGATA CGTTGATCGG AACCGTAAAT 
GTCAAGCTGC AGCCATTGGA GACCAAGTGC GAGATACACG ACACATATGA TCTAATGGAT 
GGTCGCAAGC AAGTAGGTGG AAAATTGGAA GTCAAGATAC GCGTTCGCAA TCCTATTCTC 
ACCAAGCAAA TGGAGCACAT CACCGAAAAG TGGTTGGTCT TGGATGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609488.1
CDS	123..2573
Translation	MFSRKKPEPAKRRQHDLSQFGLTEIPDDFDPSAGYGEDDGGDSDLEAELAAITGGEGAKPKPKPKAKLLPASDLDKMIADSLRDVSDDDDDDNLESDPDLLGELSGIGGLEEAEEEEPVAQPPAASEEPVQTFLPTTTVDTLSIIKQRLEMYKQAEANAKTAGDSGKARRFGRGLKTLKDLHRQAAAGKSINVDDIPPEVSVKPIGGQAPPVPAEESPAPSTPASPPPVPSRAAPDPPTPGTPVEPTTSVAPTSPPNPLVTQMRSRQTDYKAAALQSKRSGDISTALQFLKVVKQFDVVIKMCEDGQEVDLSDMPPPPAEFLEFLKKMQEEAAAEAVAEPTAAPEPTPVAPAPVLAAATNMLEALQQRLEKYQSVEAAAKAENNSGKARRFGRIVKQYEDAIKLYKAGKPVPYDELPVPPGFGPLPTADAAPVAPTPSLPTSPTSPPPTASTSAGGTPSSSSATTPTAPRKAPSPPKPKELTTRTSGNQQKNNIAEQQMKLLLERQKEFKLAAIEAKKAGEIDQAKEYLKIFKGFDSLLNAASSGLPVDLSTLPVPPSQRDNLEASFAIVSAEECDPTDDICEIGVRMEEQLAKQLMMCKNTRDHHKAMGDVAGMNRFENLALTVQKDLDLVRYSKRKNEPLPKFHYEKRSFNIVHCNTDLTDSELEIVVVRGISYNVANPKDVDTYVRVEFPLLNDESFKTKTNVIRDTSSPDYDERFKVDIQRTNRQFQRIFKRHGVKFEIYSRGGFLRSDTLIGTVNVKLQPLETKCEIHDTYDLMDGRKQVGGKLEVKIRVRNPILTKQMEHITEKWLVLDA

Target ORF information:

RefSeq Version	NM_135644.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (2) giant discs 1 (l(2)gd1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135644.4

ORF Insert Sequence:

ATGTTCTCCA GAAAGAAGCC AGAGCCAGCG AAAAGAAGGC AACACGATTT ATCGCAGTTC 
GGTCTTACGG AAATCCCAGA TGATTTTGAT CCCAGCGCTG GATATGGTGA GGACGATGGG 
GGTGACAGTG ATTTGGAGGC AGAGCTTGCG GCCATAACCG GCGGAGAAGG AGCCAAGCCA 
AAGCCCAAGC CCAAAGCAAA GCTGTTACCC GCCAGTGATT TGGACAAAAT GATAGCCGAC 
AGCTTGCGTG ATGTCAGCGA CGATGATGAT GATGACAATT TGGAAAGCGA TCCGGATCTT 
CTTGGCGAGC TGAGCGGAAT CGGTGGTCTA GAGGAGGCAG AGGAAGAGGA GCCTGTCGCA 
CAACCGCCAG CTGCCTCTGA GGAACCCGTC CAGACCTTTC TGCCCACGAC GACTGTGGAC 
ACATTAAGCA TTATCAAACA GCGCCTGGAA ATGTACAAGC AGGCCGAGGC CAATGCCAAG 
ACTGCCGGCG ATTCTGGCAA AGCAAGGCGA TTTGGAAGAG GCCTTAAGAC TCTGAAAGAT 
CTGCACAGGC AGGCAGCGGC TGGCAAGTCC ATTAACGTGG ATGACATTCC ACCTGAAGTC 
AGTGTAAAGC CAATTGGCGG GCAGGCTCCT CCAGTGCCTG CAGAGGAGTC ACCAGCTCCT 
TCCACACCTG CTTCCCCTCC GCCTGTGCCT AGTCGTGCAG CTCCAGATCC ACCAACCCCC 
GGCACCCCCG TGGAGCCCAC TACATCTGTG GCTCCCACAT CTCCCCCTAA TCCTCTTGTG 
ACGCAAATGC GCAGCCGCCA GACGGACTAC AAAGCTGCTG CACTGCAATC TAAGCGCAGT 
GGTGACATAT CTACTGCCCT TCAGTTTCTC AAAGTGGTCA AGCAATTTGA CGTAGTTATA 
AAAATGTGTG AAGACGGACA GGAAGTTGAT TTGAGTGACA TGCCCCCTCC ACCAGCTGAG 
TTTCTTGAAT TCCTTAAAAA AATGCAAGAA GAAGCAGCCG CAGAAGCTGT CGCAGAACCT 
ACTGCTGCCC CAGAACCCAC ACCTGTTGCT CCTGCTCCTG TTCTAGCAGC TGCAACAAAT 
ATGTTGGAGG CTCTGCAGCA ACGTTTAGAG AAATATCAAT CTGTAGAAGC GGCAGCCAAG 
GCAGAAAACA ATAGCGGAAA AGCAAGACGC TTTGGAAGGA TTGTGAAGCA ATACGAAGAT 
GCCATAAAGT TGTACAAGGC AGGCAAACCA GTGCCTTACG ATGAGCTGCC TGTGCCACCA 
GGTTTTGGAC CGCTGCCTAC GGCTGATGCT GCTCCTGTCG CGCCGACACC GTCGCTGCCC 
ACTTCGCCTA CATCTCCACC GCCAACAGCA AGTACTTCCG CAGGAGGAAC ACCCTCCAGT 
TCCAGTGCGA CGACACCTAC AGCTCCTCGA AAAGCACCTT CACCTCCTAA GCCCAAAGAG 
TTAACAACAC GCACGTCTGG CAATCAGCAG AAGAATAATA TTGCCGAGCA ACAAATGAAA 
CTGCTCCTCG AGCGCCAAAA GGAGTTTAAG CTAGCTGCTA TAGAGGCCAA AAAAGCGGGA 
GAAATAGATC AGGCGAAGGA GTACCTCAAG ATCTTCAAGG GATTCGATTC CCTTCTTAAT 
GCAGCGAGTA GTGGATTGCC AGTGGACCTC AGCACTCTTC CCGTCCCGCC TTCACAAAGG 
GATAATCTAG AAGCCTCCTT TGCCATTGTA TCTGCTGAGG AATGCGATCC GACAGATGAT 
ATCTGTGAAA TTGGTGTTCG CATGGAGGAG CAGCTGGCAA AACAACTGAT GATGTGCAAA 
AATACGCGAG ATCATCACAA AGCTATGGGA GATGTAGCAG GCATGAATCG GTTTGAAAAT 
TTAGCCTTAA CTGTGCAAAA GGATTTGGAT TTGGTGCGAT ACTCGAAACG AAAGAATGAA 
CCACTGCCAA AGTTTCACTA TGAAAAGCGT AGCTTCAACA TTGTGCATTG CAACACAGAT 
CTCACAGACA GTGAGCTTGA AATTGTTGTG GTCCGGGGAA TCAGCTACAA TGTGGCCAAT 
CCCAAGGACG TGGATACTTA TGTGCGCGTA GAGTTTCCCC TGCTCAATGA TGAATCTTTC 
AAGACTAAAA CCAATGTTAT CAGAGACACC AGCAGTCCTG ACTACGATGA ACGCTTCAAG 
GTTGACATCC AGCGGACCAA TCGTCAGTTC CAGAGAATCT TTAAGCGGCA TGGCGTCAAG 
TTTGAAATAT ACTCTAGAGG AGGTTTTCTG CGATCGGATA CGTTGATCGG AACCGTAAAT 
GTCAAGCTGC AGCCATTGGA GACCAAGTGC GAGATACACG ACACATATGA TCTAATGGAT 
GGTCGCAAGC AAGTAGGTGG AAAATTGGAA GTCAAGATAC GCGTTCGCAA TCCTATTCTC 
ACCAAGCAAA TGGAGCACAT CACCGAAAAG TGGTTGGTCT TGGATGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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l(2)gd1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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