Nup154 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Nup154 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nup154 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa16642 NM_057940.4
Latest version!
Drosophila melanogaster nucleoporin 154kD, transcript variant C (Nup154), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa16642 NM_001298898.1
Latest version!
Drosophila melanogaster nucleoporin 154kD, transcript variant D (Nup154), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa16642 NM_057939.4
Latest version!
Drosophila melanogaster nucleoporin 154kD, transcript variant A (Nup154), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa16642
    Clone ID Related Accession (Same CDS sequence) NM_057939.4 , NM_001298898.1 , NM_057940.4
    Accession Version NM_057939.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nucleoporin 154kD, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057939.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGACTCTTC CGCAAGCACA GCTGGACTTT TTCACGACGG CGACCAGTAT GCTGGAATGG 
    CATTACAATC TGGAGAGAAA TAAGCCGGGT CTGCTGGAAC TGACCGGCGT TAGCCAGCAT
    GGGCGGGCCA CGATGAGCGG ACTTAATGAC TACGATTACC AGAGCCTGTC GTTTTTGAAA
    TCGGATAGAA CACATAACCT ACAACAAATG CGAACGGTGA CCAAGTCGGC TATTCCCAAC
    GAGATTCTGG AGCACTTTAA GCACATAAAA TGCCACTGCA CCATGGGCCT CTTTCCGGAA
    ATCGGACGTG CCTGGCTGAC TATCGATTCG GAAATCTACA TCTGGACGTT CAATCAGACG
    AGGGATGTGG CGTACTACGA TGGCCTCAGT CATCTGATCG TAAGCGTTGG CCTGGTGAAA
    CCAAAACCCG GCGTCTTTGT GCAGGACGTC AAGTACCTCC TGGTTCTCAC TACGCCCATT
    GAGGTCATTG TCCTGGGCGT GACATTCGGT GAGAGCTCGT ACAACGAAAT GCAGCTGATG
    AACCGGCCAG TTTTTGTGAT TGGTACCGAC AACGTGTCCA TCAGCGTCAT CAAAGGAACG
    GATGACGGCC GCATCTTTTT GGGCGGTCGC GATGGCTGCC TCTATGAAAT TTACTATCAG
    GCCGAGTCCA GTTGGTTTGG CAAGCGATGC AAGAAAATAA ATCTCTCCCA AGGACTGGTC
    TCCTATATGG TTCCCAGTTT TTTGAAGGTC TTTTCGGAAG TGGATCCAAT CGAACACATT
    GAGATCGATA ACAGTCGCAA GCTTCTCTAT GTCCTTACGG AAAAAGGTGT CATTGAGGCT
    TGGGACATCA GTACGAGTTA CACGACAGCT CGAAGACTAG GTCGGATTAC CCAGAACGAT
    ATTACGAATC AAGCAGTTAG TCTGATCACG ACTGTGGACC CGTCCATTTT TAAAAGTGTG
    AAAGCCATCT GCCCTCTGAG CGCCGACGAT GCCGACAAAC TACATCTTGT GGCCGTTACT
    CAGTGTGGTG TGCGGCTCTT TTTCTCCACA ACATCGCTAA ATGTGAAGCA ACAATTTGGT
    CCCGCGGTTC CTTGCAGTCC AGGTGAGAAT ACAGGATTCG GTCAGCCAGC AGTTCAGCCA
    CCACTGTCTC CAAATGCTGA GGCTCCTAAG GGACTTTATC TGCTCCACGT GCGTCTACCA
    CCCGGCTACA CCCCAAATGC CACCACCAAC AAACCAAAGC AGGTCCATGC CGCTCACTAC
    ACGGAGGGAA CCATGTTGAT GATCACAACG CAACAGCATG AACAGGATCT CCTCTGGTCG
    CTCAGCTCTG CCCCATCGGT TAACTTTACC TACCTGGTGG AGTCGACGGC TCTGGAGAGC
    CTGGATGGAG TTGTTTGGGG ATTGGCTGAG GTACATGAAC CGTCGACGCC ACAACGAAAG
    TCACCCTTAA ATAGTGCCCG ACACGCCCGC AAAGTAGCCC TGCTCACCAA CCAAGGCACA
    CATATCATAG AGGTGCTTAA AATGGTGGAC GTACTGCGTC AGATCCTTCT GTCGTGCAAT
    GGCCCACACC ACGAGGAGGT GAAGATGTTC TTCCAGTCGC AGAATCAGCG AGAGGCATGT
    GTCACGGCTC TGCTGCTGGC CACATCGGAT ACTTATCGTG GCAGTGACGT TGCTTTGTGG
    GCCGCCCAGG CGTTTATGCT GTACGGCGGA GAGCCATGCT ATCAGCATCA AAAGTTCCTG
    AACGCCAGCA ACCGCAATAT GGCTAATCAA ACTCTAGGCC CGAATACGAC GAATGTAAGG
    GAGCGTCAAT CCATGTTTAT GTCCACGCCA ATGCCGAATA GCGTGGCAAA TAGTCCGGTT
    GGTTTTCCTG GATCGCAGTT CAATCAGCCC ATAAGTCCAA TTGGAAATAT GCAGCCACCA
    CAGGTGGCAG TTAGTAACGA GAATTCCCCA ATCGTTTTCT CGGCCAAACA CGACGGACTT
    TACATGTATG TCTCCCGCAT GCTGCATTCT GTTTGGCAAA TGCGCTGTGT AAATGAGCAA
    TTTTGCTCTA ATCTAAGTCA GAGTGAGTGT GCCCTCTTGC TATCCGATCT GCGTTCCCTG
    CGTAGCTTTC TGGAAGTGCA CTCTGTGCAC GATATTTCCT CGACTACTCG AGTGTCATTT
    GATAACCATT TGGATAGAAC CAATTCATAT AACACCATCA TGATGGGCAA CACTTTGCTG
    CCAATTCCTG AGCAACGCGT ATTGTCCGAG CAGGCTCAAG TAGAGGAAAC ACGTTCGCTT
    TCCGCTCTCA ACCTATTTGT CAAACATGCA TGTGAGGTTA TATCGCTGTG GAACATCCTA
    AACTCGCATT CCTTCCAGCT GATATGCGTG CAACTCAGTC CGGAACATCA GAAGTTGTTG
    ACATGCTCCA CCTTCCGGGA TCTTCTGATT ACACGTTCAG AGGTCTGCGC ATTCTTGATC
    ATCTCGCTGA TCAATCTGTA TCTGAAGGAC GCGGCGGGAG TGTCTGAAGT GTCCAAGAAC
    TTACGTGAGA ACTGCCCCAA CCTATACAGG CATGAGGATG ACGTGACCTA CAAGGCCACC
    GAGTTGTTGA TGAATGCAAA GAATTGCACT TCGGCAACCG AAAAGGAACA TATGCTGCGA
    ACCACCCTGC ATATGTGCAA GGAAGCTGCT CCCACATTGC CACTGCATAG TATTTGCATG
    CAATTCATAT CGGCTGACTT CTTTGAGGGA GTGATCGAGC TTTCGGCTGT TTGTGCCTCA
    AAAAGTGATC CAGAGGAGGT TGGTGTTCAT TTCTATAACA ATGGGGAACC CGCCGATGAT
    CGCGAGGGTT ACACTTGCTT TGCCACAAGG ATGGCCTACT ACAAGGAAGT GCAACTTATG
    CTGGACCATA TCTACCAGAG AGTATGCAAC AAGACGCACG TTCAGGATAA GTCTATAAAT
    CCACTTAAAG GTACGGCTAA AGCGTCCGAT GCAAAAAACG GTGCTACGCA AACGATTCCA
    AAAATAGTGG CCCATACCCT AAAGGTGAAA GACCCGCTCA TTCATATAAC GCTCTACGAA
    TGGCTGCTGG CCCACGACAT GCTCAAAGAG CTTTTAGACG TGGTTGAGCC GTCATTAGGA
    GAGTTCTTGC GGCGCAGTGT GTCGCAAAAT GTAGATAATG TCGTTCTTAT CGATTTGCTG
    TGGAAGTACT ACGAAAAGAA TAGCCATCAT TCCCAGGCAG CCCATATATT GGACAATTTG
    GCTATGACAC GCAGTGAAAA CATTAATCTG GAGCAGCGCA TTGAGTACTT GGTGCGTGCT
    GTTATGTGTA TGCGAAATGG TAACGTTGGC TCTTCATTAA GCAATGGAAT ATTCCTGAAA
    GAGCTAGAGG ATAAGCTGGA CATCGCACGA GTTCAAAAAT CAGTGCTTGC CGCGATGACA
    GAGTTGGCTA GCGATAAGCT GGAGGCAGCT ACGGCTGTTA AGGAACTAAA CTATGCGTTG
    TACGACATAA CTCAGCTATA CCAGCACTTT GCGGAGCCCT TCGACCTTTG GGAGTGCCAA
    TTGTCCATAC TCAACTGCTC GCATCACAAC GACCCCCTAC TGATCGAGTC CGTTTGGGGC
    CAGATTATCA ACAGCGTTGT GGATAAGCCG GGCACAACTA GTGAGCGCTG CAATAGGTTA
    TTCACGAAAA TCGAAATCTT GGTAAGGGAG TATGGCGAGT CTGGAGTTTG CTTTCCCTTT
    GCCTTTCTCA TAAGGGAACT GGAAGTTAAA GCCTGCCAAC TGCGATTTCC CGAGGGCATA
    GTGCCGGAAA AACTGGTGAG TATGAATTTG GACATTGAAC TGCTCCTGGA ATACTATTCG
    AGAATGATTT CAATGAACGA ACGAGTGTGG GCCAACGAGG GTAATGAGTG GCATTTAATA
    CAATCCGTCA TACGAGTGGT CTCGCTTCTG GCAGACAACG CACAATCTAT TTGGTACCGG
    TCAAAACGAC GAATTGTTGG CAAGGCTCAA GACATAGTGG CCGGCTGCCT GAATATTTGC
    TACCAAAAGC CCGACACTAA TCGTCTTCAA CATTCACTCA AGGAACTTCA GAGTCAGCTG
    CAGCGGTTGT TGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_477287.1
    CDS161..4258
    Translation

    Target ORF information:

    RefSeq Version NM_057939.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nucleoporin 154kD, transcript variant A (Nup154), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057939.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGACTCTTC CGCAAGCACA GCTGGACTTT TTCACGACGG CGACCAGTAT GCTGGAATGG 
    CATTACAATC TGGAGAGAAA TAAGCCGGGT CTGCTGGAAC TGACCGGCGT TAGCCAGCAT
    GGGCGGGCCA CGATGAGCGG ACTTAATGAC TACGATTACC AGAGCCTGTC GTTTTTGAAA
    TCGGATAGAA CACATAACCT ACAACAAATG CGAACGGTGA CCAAGTCGGC TATTCCCAAC
    GAGATTCTGG AGCACTTTAA GCACATAAAA TGCCACTGCA CCATGGGCCT CTTTCCGGAA
    ATCGGACGTG CCTGGCTGAC TATCGATTCG GAAATCTACA TCTGGACGTT CAATCAGACG
    AGGGATGTGG CGTACTACGA TGGCCTCAGT CATCTGATCG TAAGCGTTGG CCTGGTGAAA
    CCAAAACCCG GCGTCTTTGT GCAGGACGTC AAGTACCTCC TGGTTCTCAC TACGCCCATT
    GAGGTCATTG TCCTGGGCGT GACATTCGGT GAGAGCTCGT ACAACGAAAT GCAGCTGATG
    AACCGGCCAG TTTTTGTGAT TGGTACCGAC AACGTGTCCA TCAGCGTCAT CAAAGGAACG
    GATGACGGCC GCATCTTTTT GGGCGGTCGC GATGGCTGCC TCTATGAAAT TTACTATCAG
    GCCGAGTCCA GTTGGTTTGG CAAGCGATGC AAGAAAATAA ATCTCTCCCA AGGACTGGTC
    TCCTATATGG TTCCCAGTTT TTTGAAGGTC TTTTCGGAAG TGGATCCAAT CGAACACATT
    GAGATCGATA ACAGTCGCAA GCTTCTCTAT GTCCTTACGG AAAAAGGTGT CATTGAGGCT
    TGGGACATCA GTACGAGTTA CACGACAGCT CGAAGACTAG GTCGGATTAC CCAGAACGAT
    ATTACGAATC AAGCAGTTAG TCTGATCACG ACTGTGGACC CGTCCATTTT TAAAAGTGTG
    AAAGCCATCT GCCCTCTGAG CGCCGACGAT GCCGACAAAC TACATCTTGT GGCCGTTACT
    CAGTGTGGTG TGCGGCTCTT TTTCTCCACA ACATCGCTAA ATGTGAAGCA ACAATTTGGT
    CCCGCGGTTC CTTGCAGTCC AGGTGAGAAT ACAGGATTCG GTCAGCCAGC AGTTCAGCCA
    CCACTGTCTC CAAATGCTGA GGCTCCTAAG GGACTTTATC TGCTCCACGT GCGTCTACCA
    CCCGGCTACA CCCCAAATGC CACCACCAAC AAACCAAAGC AGGTCCATGC CGCTCACTAC
    ACGGAGGGAA CCATGTTGAT GATCACAACG CAACAGCATG AACAGGATCT CCTCTGGTCG
    CTCAGCTCTG CCCCATCGGT TAACTTTACC TACCTGGTGG AGTCGACGGC TCTGGAGAGC
    CTGGATGGAG TTGTTTGGGG ATTGGCTGAG GTACATGAAC CGTCGACGCC ACAACGAAAG
    TCACCCTTAA ATAGTGCCCG ACACGCCCGC AAAGTAGCCC TGCTCACCAA CCAAGGCACA
    CATATCATAG AGGTGCTTAA AATGGTGGAC GTACTGCGTC AGATCCTTCT GTCGTGCAAT
    GGCCCACACC ACGAGGAGGT GAAGATGTTC TTCCAGTCGC AGAATCAGCG AGAGGCATGT
    GTCACGGCTC TGCTGCTGGC CACATCGGAT ACTTATCGTG GCAGTGACGT TGCTTTGTGG
    GCCGCCCAGG CGTTTATGCT GTACGGCGGA GAGCCATGCT ATCAGCATCA AAAGTTCCTG
    AACGCCAGCA ACCGCAATAT GGCTAATCAA ACTCTAGGCC CGAATACGAC GAATGTAAGG
    GAGCGTCAAT CCATGTTTAT GTCCACGCCA ATGCCGAATA GCGTGGCAAA TAGTCCGGTT
    GGTTTTCCTG GATCGCAGTT CAATCAGCCC ATAAGTCCAA TTGGAAATAT GCAGCCACCA
    CAGGTGGCAG TTAGTAACGA GAATTCCCCA ATCGTTTTCT CGGCCAAACA CGACGGACTT
    TACATGTATG TCTCCCGCAT GCTGCATTCT GTTTGGCAAA TGCGCTGTGT AAATGAGCAA
    TTTTGCTCTA ATCTAAGTCA GAGTGAGTGT GCCCTCTTGC TATCCGATCT GCGTTCCCTG
    CGTAGCTTTC TGGAAGTGCA CTCTGTGCAC GATATTTCCT CGACTACTCG AGTGTCATTT
    GATAACCATT TGGATAGAAC CAATTCATAT AACACCATCA TGATGGGCAA CACTTTGCTG
    CCAATTCCTG AGCAACGCGT ATTGTCCGAG CAGGCTCAAG TAGAGGAAAC ACGTTCGCTT
    TCCGCTCTCA ACCTATTTGT CAAACATGCA TGTGAGGTTA TATCGCTGTG GAACATCCTA
    AACTCGCATT CCTTCCAGCT GATATGCGTG CAACTCAGTC CGGAACATCA GAAGTTGTTG
    ACATGCTCCA CCTTCCGGGA TCTTCTGATT ACACGTTCAG AGGTCTGCGC ATTCTTGATC
    ATCTCGCTGA TCAATCTGTA TCTGAAGGAC GCGGCGGGAG TGTCTGAAGT GTCCAAGAAC
    TTACGTGAGA ACTGCCCCAA CCTATACAGG CATGAGGATG ACGTGACCTA CAAGGCCACC
    GAGTTGTTGA TGAATGCAAA GAATTGCACT TCGGCAACCG AAAAGGAACA TATGCTGCGA
    ACCACCCTGC ATATGTGCAA GGAAGCTGCT CCCACATTGC CACTGCATAG TATTTGCATG
    CAATTCATAT CGGCTGACTT CTTTGAGGGA GTGATCGAGC TTTCGGCTGT TTGTGCCTCA
    AAAAGTGATC CAGAGGAGGT TGGTGTTCAT TTCTATAACA ATGGGGAACC CGCCGATGAT
    CGCGAGGGTT ACACTTGCTT TGCCACAAGG ATGGCCTACT ACAAGGAAGT GCAACTTATG
    CTGGACCATA TCTACCAGAG AGTATGCAAC AAGACGCACG TTCAGGATAA GTCTATAAAT
    CCACTTAAAG GTACGGCTAA AGCGTCCGAT GCAAAAAACG GTGCTACGCA AACGATTCCA
    AAAATAGTGG CCCATACCCT AAAGGTGAAA GACCCGCTCA TTCATATAAC GCTCTACGAA
    TGGCTGCTGG CCCACGACAT GCTCAAAGAG CTTTTAGACG TGGTTGAGCC GTCATTAGGA
    GAGTTCTTGC GGCGCAGTGT GTCGCAAAAT GTAGATAATG TCGTTCTTAT CGATTTGCTG
    TGGAAGTACT ACGAAAAGAA TAGCCATCAT TCCCAGGCAG CCCATATATT GGACAATTTG
    GCTATGACAC GCAGTGAAAA CATTAATCTG GAGCAGCGCA TTGAGTACTT GGTGCGTGCT
    GTTATGTGTA TGCGAAATGG TAACGTTGGC TCTTCATTAA GCAATGGAAT ATTCCTGAAA
    GAGCTAGAGG ATAAGCTGGA CATCGCACGA GTTCAAAAAT CAGTGCTTGC CGCGATGACA
    GAGTTGGCTA GCGATAAGCT GGAGGCAGCT ACGGCTGTTA AGGAACTAAA CTATGCGTTG
    TACGACATAA CTCAGCTATA CCAGCACTTT GCGGAGCCCT TCGACCTTTG GGAGTGCCAA
    TTGTCCATAC TCAACTGCTC GCATCACAAC GACCCCCTAC TGATCGAGTC CGTTTGGGGC
    CAGATTATCA ACAGCGTTGT GGATAAGCCG GGCACAACTA GTGAGCGCTG CAATAGGTTA
    TTCACGAAAA TCGAAATCTT GGTAAGGGAG TATGGCGAGT CTGGAGTTTG CTTTCCCTTT
    GCCTTTCTCA TAAGGGAACT GGAAGTTAAA GCCTGCCAAC TGCGATTTCC CGAGGGCATA
    GTGCCGGAAA AACTGGTGAG TATGAATTTG GACATTGAAC TGCTCCTGGA ATACTATTCG
    AGAATGATTT CAATGAACGA ACGAGTGTGG GCCAACGAGG GTAATGAGTG GCATTTAATA
    CAATCCGTCA TACGAGTGGT CTCGCTTCTG GCAGACAACG CACAATCTAT TTGGTACCGG
    TCAAAACGAC GAATTGTTGG CAAGGCTCAA GACATAGTGG CCGGCTGCCT GAATATTTGC
    TACCAAAAGC CCGACACTAA TCGTCTTCAA CATTCACTCA AGGAACTTCA GAGTCAGCTG
    CAGCGGTTGT TGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16642
    Clone ID Related Accession (Same CDS sequence) NM_057939.4 , NM_001298898.1 , NM_057940.4
    Accession Version NM_001298898.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nucleoporin 154kD, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGACTCTTC CGCAAGCACA GCTGGACTTT TTCACGACGG CGACCAGTAT GCTGGAATGG 
    CATTACAATC TGGAGAGAAA TAAGCCGGGT CTGCTGGAAC TGACCGGCGT TAGCCAGCAT
    GGGCGGGCCA CGATGAGCGG ACTTAATGAC TACGATTACC AGAGCCTGTC GTTTTTGAAA
    TCGGATAGAA CACATAACCT ACAACAAATG CGAACGGTGA CCAAGTCGGC TATTCCCAAC
    GAGATTCTGG AGCACTTTAA GCACATAAAA TGCCACTGCA CCATGGGCCT CTTTCCGGAA
    ATCGGACGTG CCTGGCTGAC TATCGATTCG GAAATCTACA TCTGGACGTT CAATCAGACG
    AGGGATGTGG CGTACTACGA TGGCCTCAGT CATCTGATCG TAAGCGTTGG CCTGGTGAAA
    CCAAAACCCG GCGTCTTTGT GCAGGACGTC AAGTACCTCC TGGTTCTCAC TACGCCCATT
    GAGGTCATTG TCCTGGGCGT GACATTCGGT GAGAGCTCGT ACAACGAAAT GCAGCTGATG
    AACCGGCCAG TTTTTGTGAT TGGTACCGAC AACGTGTCCA TCAGCGTCAT CAAAGGAACG
    GATGACGGCC GCATCTTTTT GGGCGGTCGC GATGGCTGCC TCTATGAAAT TTACTATCAG
    GCCGAGTCCA GTTGGTTTGG CAAGCGATGC AAGAAAATAA ATCTCTCCCA AGGACTGGTC
    TCCTATATGG TTCCCAGTTT TTTGAAGGTC TTTTCGGAAG TGGATCCAAT CGAACACATT
    GAGATCGATA ACAGTCGCAA GCTTCTCTAT GTCCTTACGG AAAAAGGTGT CATTGAGGCT
    TGGGACATCA GTACGAGTTA CACGACAGCT CGAAGACTAG GTCGGATTAC CCAGAACGAT
    ATTACGAATC AAGCAGTTAG TCTGATCACG ACTGTGGACC CGTCCATTTT TAAAAGTGTG
    AAAGCCATCT GCCCTCTGAG CGCCGACGAT GCCGACAAAC TACATCTTGT GGCCGTTACT
    CAGTGTGGTG TGCGGCTCTT TTTCTCCACA ACATCGCTAA ATGTGAAGCA ACAATTTGGT
    CCCGCGGTTC CTTGCAGTCC AGGTGAGAAT ACAGGATTCG GTCAGCCAGC AGTTCAGCCA
    CCACTGTCTC CAAATGCTGA GGCTCCTAAG GGACTTTATC TGCTCCACGT GCGTCTACCA
    CCCGGCTACA CCCCAAATGC CACCACCAAC AAACCAAAGC AGGTCCATGC CGCTCACTAC
    ACGGAGGGAA CCATGTTGAT GATCACAACG CAACAGCATG AACAGGATCT CCTCTGGTCG
    CTCAGCTCTG CCCCATCGGT TAACTTTACC TACCTGGTGG AGTCGACGGC TCTGGAGAGC
    CTGGATGGAG TTGTTTGGGG ATTGGCTGAG GTACATGAAC CGTCGACGCC ACAACGAAAG
    TCACCCTTAA ATAGTGCCCG ACACGCCCGC AAAGTAGCCC TGCTCACCAA CCAAGGCACA
    CATATCATAG AGGTGCTTAA AATGGTGGAC GTACTGCGTC AGATCCTTCT GTCGTGCAAT
    GGCCCACACC ACGAGGAGGT GAAGATGTTC TTCCAGTCGC AGAATCAGCG AGAGGCATGT
    GTCACGGCTC TGCTGCTGGC CACATCGGAT ACTTATCGTG GCAGTGACGT TGCTTTGTGG
    GCCGCCCAGG CGTTTATGCT GTACGGCGGA GAGCCATGCT ATCAGCATCA AAAGTTCCTG
    AACGCCAGCA ACCGCAATAT GGCTAATCAA ACTCTAGGCC CGAATACGAC GAATGTAAGG
    GAGCGTCAAT CCATGTTTAT GTCCACGCCA ATGCCGAATA GCGTGGCAAA TAGTCCGGTT
    GGTTTTCCTG GATCGCAGTT CAATCAGCCC ATAAGTCCAA TTGGAAATAT GCAGCCACCA
    CAGGTGGCAG TTAGTAACGA GAATTCCCCA ATCGTTTTCT CGGCCAAACA CGACGGACTT
    TACATGTATG TCTCCCGCAT GCTGCATTCT GTTTGGCAAA TGCGCTGTGT AAATGAGCAA
    TTTTGCTCTA ATCTAAGTCA GAGTGAGTGT GCCCTCTTGC TATCCGATCT GCGTTCCCTG
    CGTAGCTTTC TGGAAGTGCA CTCTGTGCAC GATATTTCCT CGACTACTCG AGTGTCATTT
    GATAACCATT TGGATAGAAC CAATTCATAT AACACCATCA TGATGGGCAA CACTTTGCTG
    CCAATTCCTG AGCAACGCGT ATTGTCCGAG CAGGCTCAAG TAGAGGAAAC ACGTTCGCTT
    TCCGCTCTCA ACCTATTTGT CAAACATGCA TGTGAGGTTA TATCGCTGTG GAACATCCTA
    AACTCGCATT CCTTCCAGCT GATATGCGTG CAACTCAGTC CGGAACATCA GAAGTTGTTG
    ACATGCTCCA CCTTCCGGGA TCTTCTGATT ACACGTTCAG AGGTCTGCGC ATTCTTGATC
    ATCTCGCTGA TCAATCTGTA TCTGAAGGAC GCGGCGGGAG TGTCTGAAGT GTCCAAGAAC
    TTACGTGAGA ACTGCCCCAA CCTATACAGG CATGAGGATG ACGTGACCTA CAAGGCCACC
    GAGTTGTTGA TGAATGCAAA GAATTGCACT TCGGCAACCG AAAAGGAACA TATGCTGCGA
    ACCACCCTGC ATATGTGCAA GGAAGCTGCT CCCACATTGC CACTGCATAG TATTTGCATG
    CAATTCATAT CGGCTGACTT CTTTGAGGGA GTGATCGAGC TTTCGGCTGT TTGTGCCTCA
    AAAAGTGATC CAGAGGAGGT TGGTGTTCAT TTCTATAACA ATGGGGAACC CGCCGATGAT
    CGCGAGGGTT ACACTTGCTT TGCCACAAGG ATGGCCTACT ACAAGGAAGT GCAACTTATG
    CTGGACCATA TCTACCAGAG AGTATGCAAC AAGACGCACG TTCAGGATAA GTCTATAAAT
    CCACTTAAAG GTACGGCTAA AGCGTCCGAT GCAAAAAACG GTGCTACGCA AACGATTCCA
    AAAATAGTGG CCCATACCCT AAAGGTGAAA GACCCGCTCA TTCATATAAC GCTCTACGAA
    TGGCTGCTGG CCCACGACAT GCTCAAAGAG CTTTTAGACG TGGTTGAGCC GTCATTAGGA
    GAGTTCTTGC GGCGCAGTGT GTCGCAAAAT GTAGATAATG TCGTTCTTAT CGATTTGCTG
    TGGAAGTACT ACGAAAAGAA TAGCCATCAT TCCCAGGCAG CCCATATATT GGACAATTTG
    GCTATGACAC GCAGTGAAAA CATTAATCTG GAGCAGCGCA TTGAGTACTT GGTGCGTGCT
    GTTATGTGTA TGCGAAATGG TAACGTTGGC TCTTCATTAA GCAATGGAAT ATTCCTGAAA
    GAGCTAGAGG ATAAGCTGGA CATCGCACGA GTTCAAAAAT CAGTGCTTGC CGCGATGACA
    GAGTTGGCTA GCGATAAGCT GGAGGCAGCT ACGGCTGTTA AGGAACTAAA CTATGCGTTG
    TACGACATAA CTCAGCTATA CCAGCACTTT GCGGAGCCCT TCGACCTTTG GGAGTGCCAA
    TTGTCCATAC TCAACTGCTC GCATCACAAC GACCCCCTAC TGATCGAGTC CGTTTGGGGC
    CAGATTATCA ACAGCGTTGT GGATAAGCCG GGCACAACTA GTGAGCGCTG CAATAGGTTA
    TTCACGAAAA TCGAAATCTT GGTAAGGGAG TATGGCGAGT CTGGAGTTTG CTTTCCCTTT
    GCCTTTCTCA TAAGGGAACT GGAAGTTAAA GCCTGCCAAC TGCGATTTCC CGAGGGCATA
    GTGCCGGAAA AACTGGTGAG TATGAATTTG GACATTGAAC TGCTCCTGGA ATACTATTCG
    AGAATGATTT CAATGAACGA ACGAGTGTGG GCCAACGAGG GTAATGAGTG GCATTTAATA
    CAATCCGTCA TACGAGTGGT CTCGCTTCTG GCAGACAACG CACAATCTAT TTGGTACCGG
    TCAAAACGAC GAATTGTTGG CAAGGCTCAA GACATAGTGG CCGGCTGCCT GAATATTTGC
    TACCAAAAGC CCGACACTAA TCGTCTTCAA CATTCACTCA AGGAACTTCA GAGTCAGCTG
    CAGCGGTTGT TGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001285827.1
    CDS161..4258
    Translation

    Target ORF information:

    RefSeq Version NM_001298898.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nucleoporin 154kD, transcript variant D (Nup154), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001298898.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGACTCTTC CGCAAGCACA GCTGGACTTT TTCACGACGG CGACCAGTAT GCTGGAATGG 
    CATTACAATC TGGAGAGAAA TAAGCCGGGT CTGCTGGAAC TGACCGGCGT TAGCCAGCAT
    GGGCGGGCCA CGATGAGCGG ACTTAATGAC TACGATTACC AGAGCCTGTC GTTTTTGAAA
    TCGGATAGAA CACATAACCT ACAACAAATG CGAACGGTGA CCAAGTCGGC TATTCCCAAC
    GAGATTCTGG AGCACTTTAA GCACATAAAA TGCCACTGCA CCATGGGCCT CTTTCCGGAA
    ATCGGACGTG CCTGGCTGAC TATCGATTCG GAAATCTACA TCTGGACGTT CAATCAGACG
    AGGGATGTGG CGTACTACGA TGGCCTCAGT CATCTGATCG TAAGCGTTGG CCTGGTGAAA
    CCAAAACCCG GCGTCTTTGT GCAGGACGTC AAGTACCTCC TGGTTCTCAC TACGCCCATT
    GAGGTCATTG TCCTGGGCGT GACATTCGGT GAGAGCTCGT ACAACGAAAT GCAGCTGATG
    AACCGGCCAG TTTTTGTGAT TGGTACCGAC AACGTGTCCA TCAGCGTCAT CAAAGGAACG
    GATGACGGCC GCATCTTTTT GGGCGGTCGC GATGGCTGCC TCTATGAAAT TTACTATCAG
    GCCGAGTCCA GTTGGTTTGG CAAGCGATGC AAGAAAATAA ATCTCTCCCA AGGACTGGTC
    TCCTATATGG TTCCCAGTTT TTTGAAGGTC TTTTCGGAAG TGGATCCAAT CGAACACATT
    GAGATCGATA ACAGTCGCAA GCTTCTCTAT GTCCTTACGG AAAAAGGTGT CATTGAGGCT
    TGGGACATCA GTACGAGTTA CACGACAGCT CGAAGACTAG GTCGGATTAC CCAGAACGAT
    ATTACGAATC AAGCAGTTAG TCTGATCACG ACTGTGGACC CGTCCATTTT TAAAAGTGTG
    AAAGCCATCT GCCCTCTGAG CGCCGACGAT GCCGACAAAC TACATCTTGT GGCCGTTACT
    CAGTGTGGTG TGCGGCTCTT TTTCTCCACA ACATCGCTAA ATGTGAAGCA ACAATTTGGT
    CCCGCGGTTC CTTGCAGTCC AGGTGAGAAT ACAGGATTCG GTCAGCCAGC AGTTCAGCCA
    CCACTGTCTC CAAATGCTGA GGCTCCTAAG GGACTTTATC TGCTCCACGT GCGTCTACCA
    CCCGGCTACA CCCCAAATGC CACCACCAAC AAACCAAAGC AGGTCCATGC CGCTCACTAC
    ACGGAGGGAA CCATGTTGAT GATCACAACG CAACAGCATG AACAGGATCT CCTCTGGTCG
    CTCAGCTCTG CCCCATCGGT TAACTTTACC TACCTGGTGG AGTCGACGGC TCTGGAGAGC
    CTGGATGGAG TTGTTTGGGG ATTGGCTGAG GTACATGAAC CGTCGACGCC ACAACGAAAG
    TCACCCTTAA ATAGTGCCCG ACACGCCCGC AAAGTAGCCC TGCTCACCAA CCAAGGCACA
    CATATCATAG AGGTGCTTAA AATGGTGGAC GTACTGCGTC AGATCCTTCT GTCGTGCAAT
    GGCCCACACC ACGAGGAGGT GAAGATGTTC TTCCAGTCGC AGAATCAGCG AGAGGCATGT
    GTCACGGCTC TGCTGCTGGC CACATCGGAT ACTTATCGTG GCAGTGACGT TGCTTTGTGG
    GCCGCCCAGG CGTTTATGCT GTACGGCGGA GAGCCATGCT ATCAGCATCA AAAGTTCCTG
    AACGCCAGCA ACCGCAATAT GGCTAATCAA ACTCTAGGCC CGAATACGAC GAATGTAAGG
    GAGCGTCAAT CCATGTTTAT GTCCACGCCA ATGCCGAATA GCGTGGCAAA TAGTCCGGTT
    GGTTTTCCTG GATCGCAGTT CAATCAGCCC ATAAGTCCAA TTGGAAATAT GCAGCCACCA
    CAGGTGGCAG TTAGTAACGA GAATTCCCCA ATCGTTTTCT CGGCCAAACA CGACGGACTT
    TACATGTATG TCTCCCGCAT GCTGCATTCT GTTTGGCAAA TGCGCTGTGT AAATGAGCAA
    TTTTGCTCTA ATCTAAGTCA GAGTGAGTGT GCCCTCTTGC TATCCGATCT GCGTTCCCTG
    CGTAGCTTTC TGGAAGTGCA CTCTGTGCAC GATATTTCCT CGACTACTCG AGTGTCATTT
    GATAACCATT TGGATAGAAC CAATTCATAT AACACCATCA TGATGGGCAA CACTTTGCTG
    CCAATTCCTG AGCAACGCGT ATTGTCCGAG CAGGCTCAAG TAGAGGAAAC ACGTTCGCTT
    TCCGCTCTCA ACCTATTTGT CAAACATGCA TGTGAGGTTA TATCGCTGTG GAACATCCTA
    AACTCGCATT CCTTCCAGCT GATATGCGTG CAACTCAGTC CGGAACATCA GAAGTTGTTG
    ACATGCTCCA CCTTCCGGGA TCTTCTGATT ACACGTTCAG AGGTCTGCGC ATTCTTGATC
    ATCTCGCTGA TCAATCTGTA TCTGAAGGAC GCGGCGGGAG TGTCTGAAGT GTCCAAGAAC
    TTACGTGAGA ACTGCCCCAA CCTATACAGG CATGAGGATG ACGTGACCTA CAAGGCCACC
    GAGTTGTTGA TGAATGCAAA GAATTGCACT TCGGCAACCG AAAAGGAACA TATGCTGCGA
    ACCACCCTGC ATATGTGCAA GGAAGCTGCT CCCACATTGC CACTGCATAG TATTTGCATG
    CAATTCATAT CGGCTGACTT CTTTGAGGGA GTGATCGAGC TTTCGGCTGT TTGTGCCTCA
    AAAAGTGATC CAGAGGAGGT TGGTGTTCAT TTCTATAACA ATGGGGAACC CGCCGATGAT
    CGCGAGGGTT ACACTTGCTT TGCCACAAGG ATGGCCTACT ACAAGGAAGT GCAACTTATG
    CTGGACCATA TCTACCAGAG AGTATGCAAC AAGACGCACG TTCAGGATAA GTCTATAAAT
    CCACTTAAAG GTACGGCTAA AGCGTCCGAT GCAAAAAACG GTGCTACGCA AACGATTCCA
    AAAATAGTGG CCCATACCCT AAAGGTGAAA GACCCGCTCA TTCATATAAC GCTCTACGAA
    TGGCTGCTGG CCCACGACAT GCTCAAAGAG CTTTTAGACG TGGTTGAGCC GTCATTAGGA
    GAGTTCTTGC GGCGCAGTGT GTCGCAAAAT GTAGATAATG TCGTTCTTAT CGATTTGCTG
    TGGAAGTACT ACGAAAAGAA TAGCCATCAT TCCCAGGCAG CCCATATATT GGACAATTTG
    GCTATGACAC GCAGTGAAAA CATTAATCTG GAGCAGCGCA TTGAGTACTT GGTGCGTGCT
    GTTATGTGTA TGCGAAATGG TAACGTTGGC TCTTCATTAA GCAATGGAAT ATTCCTGAAA
    GAGCTAGAGG ATAAGCTGGA CATCGCACGA GTTCAAAAAT CAGTGCTTGC CGCGATGACA
    GAGTTGGCTA GCGATAAGCT GGAGGCAGCT ACGGCTGTTA AGGAACTAAA CTATGCGTTG
    TACGACATAA CTCAGCTATA CCAGCACTTT GCGGAGCCCT TCGACCTTTG GGAGTGCCAA
    TTGTCCATAC TCAACTGCTC GCATCACAAC GACCCCCTAC TGATCGAGTC CGTTTGGGGC
    CAGATTATCA ACAGCGTTGT GGATAAGCCG GGCACAACTA GTGAGCGCTG CAATAGGTTA
    TTCACGAAAA TCGAAATCTT GGTAAGGGAG TATGGCGAGT CTGGAGTTTG CTTTCCCTTT
    GCCTTTCTCA TAAGGGAACT GGAAGTTAAA GCCTGCCAAC TGCGATTTCC CGAGGGCATA
    GTGCCGGAAA AACTGGTGAG TATGAATTTG GACATTGAAC TGCTCCTGGA ATACTATTCG
    AGAATGATTT CAATGAACGA ACGAGTGTGG GCCAACGAGG GTAATGAGTG GCATTTAATA
    CAATCCGTCA TACGAGTGGT CTCGCTTCTG GCAGACAACG CACAATCTAT TTGGTACCGG
    TCAAAACGAC GAATTGTTGG CAAGGCTCAA GACATAGTGG CCGGCTGCCT GAATATTTGC
    TACCAAAAGC CCGACACTAA TCGTCTTCAA CATTCACTCA AGGAACTTCA GAGTCAGCTG
    CAGCGGTTGT TGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16642
    Clone ID Related Accession (Same CDS sequence) NM_057939.4 , NM_001298898.1 , NM_057940.4
    Accession Version NM_057940.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product nucleoporin 154kD, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057940.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGACTCTTC CGCAAGCACA GCTGGACTTT TTCACGACGG CGACCAGTAT GCTGGAATGG 
    CATTACAATC TGGAGAGAAA TAAGCCGGGT CTGCTGGAAC TGACCGGCGT TAGCCAGCAT
    GGGCGGGCCA CGATGAGCGG ACTTAATGAC TACGATTACC AGAGCCTGTC GTTTTTGAAA
    TCGGATAGAA CACATAACCT ACAACAAATG CGAACGGTGA CCAAGTCGGC TATTCCCAAC
    GAGATTCTGG AGCACTTTAA GCACATAAAA TGCCACTGCA CCATGGGCCT CTTTCCGGAA
    ATCGGACGTG CCTGGCTGAC TATCGATTCG GAAATCTACA TCTGGACGTT CAATCAGACG
    AGGGATGTGG CGTACTACGA TGGCCTCAGT CATCTGATCG TAAGCGTTGG CCTGGTGAAA
    CCAAAACCCG GCGTCTTTGT GCAGGACGTC AAGTACCTCC TGGTTCTCAC TACGCCCATT
    GAGGTCATTG TCCTGGGCGT GACATTCGGT GAGAGCTCGT ACAACGAAAT GCAGCTGATG
    AACCGGCCAG TTTTTGTGAT TGGTACCGAC AACGTGTCCA TCAGCGTCAT CAAAGGAACG
    GATGACGGCC GCATCTTTTT GGGCGGTCGC GATGGCTGCC TCTATGAAAT TTACTATCAG
    GCCGAGTCCA GTTGGTTTGG CAAGCGATGC AAGAAAATAA ATCTCTCCCA AGGACTGGTC
    TCCTATATGG TTCCCAGTTT TTTGAAGGTC TTTTCGGAAG TGGATCCAAT CGAACACATT
    GAGATCGATA ACAGTCGCAA GCTTCTCTAT GTCCTTACGG AAAAAGGTGT CATTGAGGCT
    TGGGACATCA GTACGAGTTA CACGACAGCT CGAAGACTAG GTCGGATTAC CCAGAACGAT
    ATTACGAATC AAGCAGTTAG TCTGATCACG ACTGTGGACC CGTCCATTTT TAAAAGTGTG
    AAAGCCATCT GCCCTCTGAG CGCCGACGAT GCCGACAAAC TACATCTTGT GGCCGTTACT
    CAGTGTGGTG TGCGGCTCTT TTTCTCCACA ACATCGCTAA ATGTGAAGCA ACAATTTGGT
    CCCGCGGTTC CTTGCAGTCC AGGTGAGAAT ACAGGATTCG GTCAGCCAGC AGTTCAGCCA
    CCACTGTCTC CAAATGCTGA GGCTCCTAAG GGACTTTATC TGCTCCACGT GCGTCTACCA
    CCCGGCTACA CCCCAAATGC CACCACCAAC AAACCAAAGC AGGTCCATGC CGCTCACTAC
    ACGGAGGGAA CCATGTTGAT GATCACAACG CAACAGCATG AACAGGATCT CCTCTGGTCG
    CTCAGCTCTG CCCCATCGGT TAACTTTACC TACCTGGTGG AGTCGACGGC TCTGGAGAGC
    CTGGATGGAG TTGTTTGGGG ATTGGCTGAG GTACATGAAC CGTCGACGCC ACAACGAAAG
    TCACCCTTAA ATAGTGCCCG ACACGCCCGC AAAGTAGCCC TGCTCACCAA CCAAGGCACA
    CATATCATAG AGGTGCTTAA AATGGTGGAC GTACTGCGTC AGATCCTTCT GTCGTGCAAT
    GGCCCACACC ACGAGGAGGT GAAGATGTTC TTCCAGTCGC AGAATCAGCG AGAGGCATGT
    GTCACGGCTC TGCTGCTGGC CACATCGGAT ACTTATCGTG GCAGTGACGT TGCTTTGTGG
    GCCGCCCAGG CGTTTATGCT GTACGGCGGA GAGCCATGCT ATCAGCATCA AAAGTTCCTG
    AACGCCAGCA ACCGCAATAT GGCTAATCAA ACTCTAGGCC CGAATACGAC GAATGTAAGG
    GAGCGTCAAT CCATGTTTAT GTCCACGCCA ATGCCGAATA GCGTGGCAAA TAGTCCGGTT
    GGTTTTCCTG GATCGCAGTT CAATCAGCCC ATAAGTCCAA TTGGAAATAT GCAGCCACCA
    CAGGTGGCAG TTAGTAACGA GAATTCCCCA ATCGTTTTCT CGGCCAAACA CGACGGACTT
    TACATGTATG TCTCCCGCAT GCTGCATTCT GTTTGGCAAA TGCGCTGTGT AAATGAGCAA
    TTTTGCTCTA ATCTAAGTCA GAGTGAGTGT GCCCTCTTGC TATCCGATCT GCGTTCCCTG
    CGTAGCTTTC TGGAAGTGCA CTCTGTGCAC GATATTTCCT CGACTACTCG AGTGTCATTT
    GATAACCATT TGGATAGAAC CAATTCATAT AACACCATCA TGATGGGCAA CACTTTGCTG
    CCAATTCCTG AGCAACGCGT ATTGTCCGAG CAGGCTCAAG TAGAGGAAAC ACGTTCGCTT
    TCCGCTCTCA ACCTATTTGT CAAACATGCA TGTGAGGTTA TATCGCTGTG GAACATCCTA
    AACTCGCATT CCTTCCAGCT GATATGCGTG CAACTCAGTC CGGAACATCA GAAGTTGTTG
    ACATGCTCCA CCTTCCGGGA TCTTCTGATT ACACGTTCAG AGGTCTGCGC ATTCTTGATC
    ATCTCGCTGA TCAATCTGTA TCTGAAGGAC GCGGCGGGAG TGTCTGAAGT GTCCAAGAAC
    TTACGTGAGA ACTGCCCCAA CCTATACAGG CATGAGGATG ACGTGACCTA CAAGGCCACC
    GAGTTGTTGA TGAATGCAAA GAATTGCACT TCGGCAACCG AAAAGGAACA TATGCTGCGA
    ACCACCCTGC ATATGTGCAA GGAAGCTGCT CCCACATTGC CACTGCATAG TATTTGCATG
    CAATTCATAT CGGCTGACTT CTTTGAGGGA GTGATCGAGC TTTCGGCTGT TTGTGCCTCA
    AAAAGTGATC CAGAGGAGGT TGGTGTTCAT TTCTATAACA ATGGGGAACC CGCCGATGAT
    CGCGAGGGTT ACACTTGCTT TGCCACAAGG ATGGCCTACT ACAAGGAAGT GCAACTTATG
    CTGGACCATA TCTACCAGAG AGTATGCAAC AAGACGCACG TTCAGGATAA GTCTATAAAT
    CCACTTAAAG GTACGGCTAA AGCGTCCGAT GCAAAAAACG GTGCTACGCA AACGATTCCA
    AAAATAGTGG CCCATACCCT AAAGGTGAAA GACCCGCTCA TTCATATAAC GCTCTACGAA
    TGGCTGCTGG CCCACGACAT GCTCAAAGAG CTTTTAGACG TGGTTGAGCC GTCATTAGGA
    GAGTTCTTGC GGCGCAGTGT GTCGCAAAAT GTAGATAATG TCGTTCTTAT CGATTTGCTG
    TGGAAGTACT ACGAAAAGAA TAGCCATCAT TCCCAGGCAG CCCATATATT GGACAATTTG
    GCTATGACAC GCAGTGAAAA CATTAATCTG GAGCAGCGCA TTGAGTACTT GGTGCGTGCT
    GTTATGTGTA TGCGAAATGG TAACGTTGGC TCTTCATTAA GCAATGGAAT ATTCCTGAAA
    GAGCTAGAGG ATAAGCTGGA CATCGCACGA GTTCAAAAAT CAGTGCTTGC CGCGATGACA
    GAGTTGGCTA GCGATAAGCT GGAGGCAGCT ACGGCTGTTA AGGAACTAAA CTATGCGTTG
    TACGACATAA CTCAGCTATA CCAGCACTTT GCGGAGCCCT TCGACCTTTG GGAGTGCCAA
    TTGTCCATAC TCAACTGCTC GCATCACAAC GACCCCCTAC TGATCGAGTC CGTTTGGGGC
    CAGATTATCA ACAGCGTTGT GGATAAGCCG GGCACAACTA GTGAGCGCTG CAATAGGTTA
    TTCACGAAAA TCGAAATCTT GGTAAGGGAG TATGGCGAGT CTGGAGTTTG CTTTCCCTTT
    GCCTTTCTCA TAAGGGAACT GGAAGTTAAA GCCTGCCAAC TGCGATTTCC CGAGGGCATA
    GTGCCGGAAA AACTGGTGAG TATGAATTTG GACATTGAAC TGCTCCTGGA ATACTATTCG
    AGAATGATTT CAATGAACGA ACGAGTGTGG GCCAACGAGG GTAATGAGTG GCATTTAATA
    CAATCCGTCA TACGAGTGGT CTCGCTTCTG GCAGACAACG CACAATCTAT TTGGTACCGG
    TCAAAACGAC GAATTGTTGG CAAGGCTCAA GACATAGTGG CCGGCTGCCT GAATATTTGC
    TACCAAAAGC CCGACACTAA TCGTCTTCAA CATTCACTCA AGGAACTTCA GAGTCAGCTG
    CAGCGGTTGT TGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_477288.3
    CDS161..4258
    Translation

    Target ORF information:

    RefSeq Version NM_057940.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster nucleoporin 154kD, transcript variant C (Nup154), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057940.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGACTCTTC CGCAAGCACA GCTGGACTTT TTCACGACGG CGACCAGTAT GCTGGAATGG 
    CATTACAATC TGGAGAGAAA TAAGCCGGGT CTGCTGGAAC TGACCGGCGT TAGCCAGCAT
    GGGCGGGCCA CGATGAGCGG ACTTAATGAC TACGATTACC AGAGCCTGTC GTTTTTGAAA
    TCGGATAGAA CACATAACCT ACAACAAATG CGAACGGTGA CCAAGTCGGC TATTCCCAAC
    GAGATTCTGG AGCACTTTAA GCACATAAAA TGCCACTGCA CCATGGGCCT CTTTCCGGAA
    ATCGGACGTG CCTGGCTGAC TATCGATTCG GAAATCTACA TCTGGACGTT CAATCAGACG
    AGGGATGTGG CGTACTACGA TGGCCTCAGT CATCTGATCG TAAGCGTTGG CCTGGTGAAA
    CCAAAACCCG GCGTCTTTGT GCAGGACGTC AAGTACCTCC TGGTTCTCAC TACGCCCATT
    GAGGTCATTG TCCTGGGCGT GACATTCGGT GAGAGCTCGT ACAACGAAAT GCAGCTGATG
    AACCGGCCAG TTTTTGTGAT TGGTACCGAC AACGTGTCCA TCAGCGTCAT CAAAGGAACG
    GATGACGGCC GCATCTTTTT GGGCGGTCGC GATGGCTGCC TCTATGAAAT TTACTATCAG
    GCCGAGTCCA GTTGGTTTGG CAAGCGATGC AAGAAAATAA ATCTCTCCCA AGGACTGGTC
    TCCTATATGG TTCCCAGTTT TTTGAAGGTC TTTTCGGAAG TGGATCCAAT CGAACACATT
    GAGATCGATA ACAGTCGCAA GCTTCTCTAT GTCCTTACGG AAAAAGGTGT CATTGAGGCT
    TGGGACATCA GTACGAGTTA CACGACAGCT CGAAGACTAG GTCGGATTAC CCAGAACGAT
    ATTACGAATC AAGCAGTTAG TCTGATCACG ACTGTGGACC CGTCCATTTT TAAAAGTGTG
    AAAGCCATCT GCCCTCTGAG CGCCGACGAT GCCGACAAAC TACATCTTGT GGCCGTTACT
    CAGTGTGGTG TGCGGCTCTT TTTCTCCACA ACATCGCTAA ATGTGAAGCA ACAATTTGGT
    CCCGCGGTTC CTTGCAGTCC AGGTGAGAAT ACAGGATTCG GTCAGCCAGC AGTTCAGCCA
    CCACTGTCTC CAAATGCTGA GGCTCCTAAG GGACTTTATC TGCTCCACGT GCGTCTACCA
    CCCGGCTACA CCCCAAATGC CACCACCAAC AAACCAAAGC AGGTCCATGC CGCTCACTAC
    ACGGAGGGAA CCATGTTGAT GATCACAACG CAACAGCATG AACAGGATCT CCTCTGGTCG
    CTCAGCTCTG CCCCATCGGT TAACTTTACC TACCTGGTGG AGTCGACGGC TCTGGAGAGC
    CTGGATGGAG TTGTTTGGGG ATTGGCTGAG GTACATGAAC CGTCGACGCC ACAACGAAAG
    TCACCCTTAA ATAGTGCCCG ACACGCCCGC AAAGTAGCCC TGCTCACCAA CCAAGGCACA
    CATATCATAG AGGTGCTTAA AATGGTGGAC GTACTGCGTC AGATCCTTCT GTCGTGCAAT
    GGCCCACACC ACGAGGAGGT GAAGATGTTC TTCCAGTCGC AGAATCAGCG AGAGGCATGT
    GTCACGGCTC TGCTGCTGGC CACATCGGAT ACTTATCGTG GCAGTGACGT TGCTTTGTGG
    GCCGCCCAGG CGTTTATGCT GTACGGCGGA GAGCCATGCT ATCAGCATCA AAAGTTCCTG
    AACGCCAGCA ACCGCAATAT GGCTAATCAA ACTCTAGGCC CGAATACGAC GAATGTAAGG
    GAGCGTCAAT CCATGTTTAT GTCCACGCCA ATGCCGAATA GCGTGGCAAA TAGTCCGGTT
    GGTTTTCCTG GATCGCAGTT CAATCAGCCC ATAAGTCCAA TTGGAAATAT GCAGCCACCA
    CAGGTGGCAG TTAGTAACGA GAATTCCCCA ATCGTTTTCT CGGCCAAACA CGACGGACTT
    TACATGTATG TCTCCCGCAT GCTGCATTCT GTTTGGCAAA TGCGCTGTGT AAATGAGCAA
    TTTTGCTCTA ATCTAAGTCA GAGTGAGTGT GCCCTCTTGC TATCCGATCT GCGTTCCCTG
    CGTAGCTTTC TGGAAGTGCA CTCTGTGCAC GATATTTCCT CGACTACTCG AGTGTCATTT
    GATAACCATT TGGATAGAAC CAATTCATAT AACACCATCA TGATGGGCAA CACTTTGCTG
    CCAATTCCTG AGCAACGCGT ATTGTCCGAG CAGGCTCAAG TAGAGGAAAC ACGTTCGCTT
    TCCGCTCTCA ACCTATTTGT CAAACATGCA TGTGAGGTTA TATCGCTGTG GAACATCCTA
    AACTCGCATT CCTTCCAGCT GATATGCGTG CAACTCAGTC CGGAACATCA GAAGTTGTTG
    ACATGCTCCA CCTTCCGGGA TCTTCTGATT ACACGTTCAG AGGTCTGCGC ATTCTTGATC
    ATCTCGCTGA TCAATCTGTA TCTGAAGGAC GCGGCGGGAG TGTCTGAAGT GTCCAAGAAC
    TTACGTGAGA ACTGCCCCAA CCTATACAGG CATGAGGATG ACGTGACCTA CAAGGCCACC
    GAGTTGTTGA TGAATGCAAA GAATTGCACT TCGGCAACCG AAAAGGAACA TATGCTGCGA
    ACCACCCTGC ATATGTGCAA GGAAGCTGCT CCCACATTGC CACTGCATAG TATTTGCATG
    CAATTCATAT CGGCTGACTT CTTTGAGGGA GTGATCGAGC TTTCGGCTGT TTGTGCCTCA
    AAAAGTGATC CAGAGGAGGT TGGTGTTCAT TTCTATAACA ATGGGGAACC CGCCGATGAT
    CGCGAGGGTT ACACTTGCTT TGCCACAAGG ATGGCCTACT ACAAGGAAGT GCAACTTATG
    CTGGACCATA TCTACCAGAG AGTATGCAAC AAGACGCACG TTCAGGATAA GTCTATAAAT
    CCACTTAAAG GTACGGCTAA AGCGTCCGAT GCAAAAAACG GTGCTACGCA AACGATTCCA
    AAAATAGTGG CCCATACCCT AAAGGTGAAA GACCCGCTCA TTCATATAAC GCTCTACGAA
    TGGCTGCTGG CCCACGACAT GCTCAAAGAG CTTTTAGACG TGGTTGAGCC GTCATTAGGA
    GAGTTCTTGC GGCGCAGTGT GTCGCAAAAT GTAGATAATG TCGTTCTTAT CGATTTGCTG
    TGGAAGTACT ACGAAAAGAA TAGCCATCAT TCCCAGGCAG CCCATATATT GGACAATTTG
    GCTATGACAC GCAGTGAAAA CATTAATCTG GAGCAGCGCA TTGAGTACTT GGTGCGTGCT
    GTTATGTGTA TGCGAAATGG TAACGTTGGC TCTTCATTAA GCAATGGAAT ATTCCTGAAA
    GAGCTAGAGG ATAAGCTGGA CATCGCACGA GTTCAAAAAT CAGTGCTTGC CGCGATGACA
    GAGTTGGCTA GCGATAAGCT GGAGGCAGCT ACGGCTGTTA AGGAACTAAA CTATGCGTTG
    TACGACATAA CTCAGCTATA CCAGCACTTT GCGGAGCCCT TCGACCTTTG GGAGTGCCAA
    TTGTCCATAC TCAACTGCTC GCATCACAAC GACCCCCTAC TGATCGAGTC CGTTTGGGGC
    CAGATTATCA ACAGCGTTGT GGATAAGCCG GGCACAACTA GTGAGCGCTG CAATAGGTTA
    TTCACGAAAA TCGAAATCTT GGTAAGGGAG TATGGCGAGT CTGGAGTTTG CTTTCCCTTT
    GCCTTTCTCA TAAGGGAACT GGAAGTTAAA GCCTGCCAAC TGCGATTTCC CGAGGGCATA
    GTGCCGGAAA AACTGGTGAG TATGAATTTG GACATTGAAC TGCTCCTGGA ATACTATTCG
    AGAATGATTT CAATGAACGA ACGAGTGTGG GCCAACGAGG GTAATGAGTG GCATTTAATA
    CAATCCGTCA TACGAGTGGT CTCGCTTCTG GCAGACAACG CACAATCTAT TTGGTACCGG
    TCAAAACGAC GAATTGTTGG CAAGGCTCAA GACATAGTGG CCGGCTGCCT GAATATTTGC
    TACCAAAAGC CCGACACTAA TCGTCTTCAA CATTCACTCA AGGAACTTCA GAGTCAGCTG
    CAGCGGTTGT TGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.