Nup154 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nup154
Entrez Gene ID	34527
Full Name	Nucleoporin 154kD
Synonyms	BcDNA:LD21772,CG4579,Dmel\CG4579,Nup,Nup155,Nup32D,l(2)01501,l(2)10432,ms(2)zk,nup154,nup32D,tip,tlp,zk
General protein information	Preferred Names Nucleoporin 154kD Names CG4579 gene product from transcript CG4579-RD CG4579-PA CG4579-PC CG4579-PD Nup154-PA Nup154-PC Nup154-PD nucleoporin 154 nucleoporin 154kD tulipano zonder kloten
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32C1-32C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057939.4	NP_477287.1	nucleoporin 154kD, isoform A
NM_001298898.1	NP_001285827.1	nucleoporin 154kD, isoform D
NM_057940.4	NP_477288.3	nucleoporin 154kD, isoform C

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nup155	NP_446404.1
Bos taurus (cattle)	NUP155	NP_001179036.1
Drosophila melanogaster (fruit fly)	Nup154	NP_477287.1
Oryza sativa (rice)	Os06g0305200	NP_001057468.1
Danio rerio (zebrafish)	nup155	NP_956450.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007999	XP_317470.4
Caenorhabditis elegans (roundworm)	npp-8	NP_500102.3
Arabidopsis thaliana (thale cress)	NUP155	NP_172938.2
Xenopus tropicalis (tropical clawed frog)	nup155	XP_002938230.2
Homo sapiens (human)	NUP155	NP_705618.1
Pan troglodytes (chimpanzee)	NUP155	XP_517798.4
Canis lupus familiaris (dog)	NUP155	XP_536500.3
Mus musculus (house mouse)	Nup155	NP_573490.3
Gallus gallus (chicken)	NUP155	XP_425016.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The role of Nup155 in inner nuclear membrane protein localisation is not mediated through the nuclear pore complexes anchoring activity of the protein.
Title: The Nup155-mediated organisation of inner nuclear membrane proteins is independent of Nup155 anchoring to the metazoan nuclear pore complex.

Nucleoporin Nup154 is required for cell survival and proliferation - Nup154 controls nuclear localization of Mad transcription factor, but does not interact directly with it
Title: Drosophila nucleoporin Nup154 controls cell viability, proliferation and nuclear accumulation of Mad transcription factor.

We propose that Nup154 is necessary for correct nuclear pore complex functions and that the proper regulation of the actin cytoskeleton dynamics strongly relies upon nuclear pore integrity.
Title: The Drosophila nucleoporin gene nup154 is required for correct microfilament dynamics and cell death during oogenesis.

cup, nup154 double mutants exhibit much stronger oogenesis defects than single mutants
Title: nup154 genetically interacts with cup and plays a cell-type-specific function during Drosophila melanogaster egg-chamber development.

The following Nup154 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nup154 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16642	NM_057940.4 Latest version!	Drosophila melanogaster nucleoporin 154kD, transcript variant C (Nup154), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa16642	NM_001298898.1 Latest version!	Drosophila melanogaster nucleoporin 154kD, transcript variant D (Nup154), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa16642	NM_057939.4 Latest version!	Drosophila melanogaster nucleoporin 154kD, transcript variant A (Nup154), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nup154 ( NM_057939.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477287.1 Drosophila melanogaster nucleoporin 154kD, transcript variant A (Nup154), mRNA.

Price & Availability↑

CloneID	OFa16642
Clone ID Related Accession (Same CDS sequence)	NM_057939.4 , NM_001298898.1 , NM_057940.4
Accession Version	NM_057939.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin 154kD, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057939.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTCTTC CGCAAGCACA GCTGGACTTT TTCACGACGG CGACCAGTAT GCTGGAATGG 
CATTACAATC TGGAGAGAAA TAAGCCGGGT CTGCTGGAAC TGACCGGCGT TAGCCAGCAT 
GGGCGGGCCA CGATGAGCGG ACTTAATGAC TACGATTACC AGAGCCTGTC GTTTTTGAAA 
TCGGATAGAA CACATAACCT ACAACAAATG CGAACGGTGA CCAAGTCGGC TATTCCCAAC 
GAGATTCTGG AGCACTTTAA GCACATAAAA TGCCACTGCA CCATGGGCCT CTTTCCGGAA 
ATCGGACGTG CCTGGCTGAC TATCGATTCG GAAATCTACA TCTGGACGTT CAATCAGACG 
AGGGATGTGG CGTACTACGA TGGCCTCAGT CATCTGATCG TAAGCGTTGG CCTGGTGAAA 
CCAAAACCCG GCGTCTTTGT GCAGGACGTC AAGTACCTCC TGGTTCTCAC TACGCCCATT 
GAGGTCATTG TCCTGGGCGT GACATTCGGT GAGAGCTCGT ACAACGAAAT GCAGCTGATG 
AACCGGCCAG TTTTTGTGAT TGGTACCGAC AACGTGTCCA TCAGCGTCAT CAAAGGAACG 
GATGACGGCC GCATCTTTTT GGGCGGTCGC GATGGCTGCC TCTATGAAAT TTACTATCAG 
GCCGAGTCCA GTTGGTTTGG CAAGCGATGC AAGAAAATAA ATCTCTCCCA AGGACTGGTC 
TCCTATATGG TTCCCAGTTT TTTGAAGGTC TTTTCGGAAG TGGATCCAAT CGAACACATT 
GAGATCGATA ACAGTCGCAA GCTTCTCTAT GTCCTTACGG AAAAAGGTGT CATTGAGGCT 
TGGGACATCA GTACGAGTTA CACGACAGCT CGAAGACTAG GTCGGATTAC CCAGAACGAT 
ATTACGAATC AAGCAGTTAG TCTGATCACG ACTGTGGACC CGTCCATTTT TAAAAGTGTG 
AAAGCCATCT GCCCTCTGAG CGCCGACGAT GCCGACAAAC TACATCTTGT GGCCGTTACT 
CAGTGTGGTG TGCGGCTCTT TTTCTCCACA ACATCGCTAA ATGTGAAGCA ACAATTTGGT 
CCCGCGGTTC CTTGCAGTCC AGGTGAGAAT ACAGGATTCG GTCAGCCAGC AGTTCAGCCA 
CCACTGTCTC CAAATGCTGA GGCTCCTAAG GGACTTTATC TGCTCCACGT GCGTCTACCA 
CCCGGCTACA CCCCAAATGC CACCACCAAC AAACCAAAGC AGGTCCATGC CGCTCACTAC 
ACGGAGGGAA CCATGTTGAT GATCACAACG CAACAGCATG AACAGGATCT CCTCTGGTCG 
CTCAGCTCTG CCCCATCGGT TAACTTTACC TACCTGGTGG AGTCGACGGC TCTGGAGAGC 
CTGGATGGAG TTGTTTGGGG ATTGGCTGAG GTACATGAAC CGTCGACGCC ACAACGAAAG 
TCACCCTTAA ATAGTGCCCG ACACGCCCGC AAAGTAGCCC TGCTCACCAA CCAAGGCACA 
CATATCATAG AGGTGCTTAA AATGGTGGAC GTACTGCGTC AGATCCTTCT GTCGTGCAAT 
GGCCCACACC ACGAGGAGGT GAAGATGTTC TTCCAGTCGC AGAATCAGCG AGAGGCATGT 
GTCACGGCTC TGCTGCTGGC CACATCGGAT ACTTATCGTG GCAGTGACGT TGCTTTGTGG 
GCCGCCCAGG CGTTTATGCT GTACGGCGGA GAGCCATGCT ATCAGCATCA AAAGTTCCTG 
AACGCCAGCA ACCGCAATAT GGCTAATCAA ACTCTAGGCC CGAATACGAC GAATGTAAGG 
GAGCGTCAAT CCATGTTTAT GTCCACGCCA ATGCCGAATA GCGTGGCAAA TAGTCCGGTT 
GGTTTTCCTG GATCGCAGTT CAATCAGCCC ATAAGTCCAA TTGGAAATAT GCAGCCACCA 
CAGGTGGCAG TTAGTAACGA GAATTCCCCA ATCGTTTTCT CGGCCAAACA CGACGGACTT 
TACATGTATG TCTCCCGCAT GCTGCATTCT GTTTGGCAAA TGCGCTGTGT AAATGAGCAA 
TTTTGCTCTA ATCTAAGTCA GAGTGAGTGT GCCCTCTTGC TATCCGATCT GCGTTCCCTG 
CGTAGCTTTC TGGAAGTGCA CTCTGTGCAC GATATTTCCT CGACTACTCG AGTGTCATTT 
GATAACCATT TGGATAGAAC CAATTCATAT AACACCATCA TGATGGGCAA CACTTTGCTG 
CCAATTCCTG AGCAACGCGT ATTGTCCGAG CAGGCTCAAG TAGAGGAAAC ACGTTCGCTT 
TCCGCTCTCA ACCTATTTGT CAAACATGCA TGTGAGGTTA TATCGCTGTG GAACATCCTA 
AACTCGCATT CCTTCCAGCT GATATGCGTG CAACTCAGTC CGGAACATCA GAAGTTGTTG 
ACATGCTCCA CCTTCCGGGA TCTTCTGATT ACACGTTCAG AGGTCTGCGC ATTCTTGATC 
ATCTCGCTGA TCAATCTGTA TCTGAAGGAC GCGGCGGGAG TGTCTGAAGT GTCCAAGAAC 
TTACGTGAGA ACTGCCCCAA CCTATACAGG CATGAGGATG ACGTGACCTA CAAGGCCACC 
GAGTTGTTGA TGAATGCAAA GAATTGCACT TCGGCAACCG AAAAGGAACA TATGCTGCGA 
ACCACCCTGC ATATGTGCAA GGAAGCTGCT CCCACATTGC CACTGCATAG TATTTGCATG 
CAATTCATAT CGGCTGACTT CTTTGAGGGA GTGATCGAGC TTTCGGCTGT TTGTGCCTCA 
AAAAGTGATC CAGAGGAGGT TGGTGTTCAT TTCTATAACA ATGGGGAACC CGCCGATGAT 
CGCGAGGGTT ACACTTGCTT TGCCACAAGG ATGGCCTACT ACAAGGAAGT GCAACTTATG 
CTGGACCATA TCTACCAGAG AGTATGCAAC AAGACGCACG TTCAGGATAA GTCTATAAAT 
CCACTTAAAG GTACGGCTAA AGCGTCCGAT GCAAAAAACG GTGCTACGCA AACGATTCCA 
AAAATAGTGG CCCATACCCT AAAGGTGAAA GACCCGCTCA TTCATATAAC GCTCTACGAA 
TGGCTGCTGG CCCACGACAT GCTCAAAGAG CTTTTAGACG TGGTTGAGCC GTCATTAGGA 
GAGTTCTTGC GGCGCAGTGT GTCGCAAAAT GTAGATAATG TCGTTCTTAT CGATTTGCTG 
TGGAAGTACT ACGAAAAGAA TAGCCATCAT TCCCAGGCAG CCCATATATT GGACAATTTG 
GCTATGACAC GCAGTGAAAA CATTAATCTG GAGCAGCGCA TTGAGTACTT GGTGCGTGCT 
GTTATGTGTA TGCGAAATGG TAACGTTGGC TCTTCATTAA GCAATGGAAT ATTCCTGAAA 
GAGCTAGAGG ATAAGCTGGA CATCGCACGA GTTCAAAAAT CAGTGCTTGC CGCGATGACA 
GAGTTGGCTA GCGATAAGCT GGAGGCAGCT ACGGCTGTTA AGGAACTAAA CTATGCGTTG 
TACGACATAA CTCAGCTATA CCAGCACTTT GCGGAGCCCT TCGACCTTTG GGAGTGCCAA 
TTGTCCATAC TCAACTGCTC GCATCACAAC GACCCCCTAC TGATCGAGTC CGTTTGGGGC 
CAGATTATCA ACAGCGTTGT GGATAAGCCG GGCACAACTA GTGAGCGCTG CAATAGGTTA 
TTCACGAAAA TCGAAATCTT GGTAAGGGAG TATGGCGAGT CTGGAGTTTG CTTTCCCTTT 
GCCTTTCTCA TAAGGGAACT GGAAGTTAAA GCCTGCCAAC TGCGATTTCC CGAGGGCATA 
GTGCCGGAAA AACTGGTGAG TATGAATTTG GACATTGAAC TGCTCCTGGA ATACTATTCG 
AGAATGATTT CAATGAACGA ACGAGTGTGG GCCAACGAGG GTAATGAGTG GCATTTAATA 
CAATCCGTCA TACGAGTGGT CTCGCTTCTG GCAGACAACG CACAATCTAT TTGGTACCGG 
TCAAAACGAC GAATTGTTGG CAAGGCTCAA GACATAGTGG CCGGCTGCCT GAATATTTGC 
TACCAAAAGC CCGACACTAA TCGTCTTCAA CATTCACTCA AGGAACTTCA GAGTCAGCTG 
CAGCGGTTGT TGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477287.1
CDS	161..4258
Translation	MTLPQAQLDFFTTATSMLEWHYNLERNKPGLLELTGVSQHGRATMSGLNDYDYQSLSFLKSDRTHNLQQMRTVTKSAIPNEILEHFKHIKCHCTMGLFPEIGRAWLTIDSEIYIWTFNQTRDVAYYDGLSHLIVSVGLVKPKPGVFVQDVKYLLVLTTPIEVIVLGVTFGESSYNEMQLMNRPVFVIGTDNVSISVIKGTDDGRIFLGGRDGCLYEIYYQAESSWFGKRCKKINLSQGLVSYMVPSFLKVFSEVDPIEHIEIDNSRKLLYVLTEKGVIEAWDISTSYTTARRLGRITQNDITNQAVSLITTVDPSIFKSVKAICPLSADDADKLHLVAVTQCGVRLFFSTTSLNVKQQFGPAVPCSPGENTGFGQPAVQPPLSPNAEAPKGLYLLHVRLPPGYTPNATTNKPKQVHAAHYTEGTMLMITTQQHEQDLLWSLSSAPSVNFTYLVESTALESLDGVVWGLAEVHEPSTPQRKSPLNSARHARKVALLTNQGTHIIEVLKMVDVLRQILLSCNGPHHEEVKMFFQSQNQREACVTALLLATSDTYRGSDVALWAAQAFMLYGGEPCYQHQKFLNASNRNMANQTLGPNTTNVRERQSMFMSTPMPNSVANSPVGFPGSQFNQPISPIGNMQPPQVAVSNENSPIVFSAKHDGLYMYVSRMLHSVWQMRCVNEQFCSNLSQSECALLLSDLRSLRSFLEVHSVHDISSTTRVSFDNHLDRTNSYNTIMMGNTLLPIPEQRVLSEQAQVEETRSLSALNLFVKHACEVISLWNILNSHSFQLICVQLSPEHQKLLTCSTFRDLLITRSEVCAFLIISLINLYLKDAAGVSEVSKNLRENCPNLYRHEDDVTYKATELLMNAKNCTSATEKEHMLRTTLHMCKEAAPTLPLHSICMQFISADFFEGVIELSAVCASKSDPEEVGVHFYNNGEPADDREGYTCFATRMAYYKEVQLMLDHIYQRVCNKTHVQDKSINPLKGTAKASDAKNGATQTIPKIVAHTLKVKDPLIHITLYEWLLAHDMLKELLDVVEPSLGEFLRRSVSQNVDNVVLIDLLWKYYEKNSHHSQAAHILDNLAMTRSENINLEQRIEYLVRAVMCMRNGNVGSSLSNGIFLKELEDKLDIARVQKSVLAAMTELASDKLEAATAVKELNYALYDITQLYQHFAEPFDLWECQLSILNCSHHNDPLLIESVWGQIINSVVDKPGTTSERCNRLFTKIEILVREYGESGVCFPFAFLIRELEVKACQLRFPEGIVPEKLVSMNLDIELLLEYYSRMISMNERVWANEGNEWHLIQSVIRVVSLLADNAQSIWYRSKRRIVGKAQDIVAGCLNICYQKPDTNRLQHSLKELQSQLQRLLI

Target ORF information:

RefSeq Version	NM_057939.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin 154kD, transcript variant A (Nup154), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057939.4

ORF Insert Sequence:

ATGACTCTTC CGCAAGCACA GCTGGACTTT TTCACGACGG CGACCAGTAT GCTGGAATGG 
CATTACAATC TGGAGAGAAA TAAGCCGGGT CTGCTGGAAC TGACCGGCGT TAGCCAGCAT 
GGGCGGGCCA CGATGAGCGG ACTTAATGAC TACGATTACC AGAGCCTGTC GTTTTTGAAA 
TCGGATAGAA CACATAACCT ACAACAAATG CGAACGGTGA CCAAGTCGGC TATTCCCAAC 
GAGATTCTGG AGCACTTTAA GCACATAAAA TGCCACTGCA CCATGGGCCT CTTTCCGGAA 
ATCGGACGTG CCTGGCTGAC TATCGATTCG GAAATCTACA TCTGGACGTT CAATCAGACG 
AGGGATGTGG CGTACTACGA TGGCCTCAGT CATCTGATCG TAAGCGTTGG CCTGGTGAAA 
CCAAAACCCG GCGTCTTTGT GCAGGACGTC AAGTACCTCC TGGTTCTCAC TACGCCCATT 
GAGGTCATTG TCCTGGGCGT GACATTCGGT GAGAGCTCGT ACAACGAAAT GCAGCTGATG 
AACCGGCCAG TTTTTGTGAT TGGTACCGAC AACGTGTCCA TCAGCGTCAT CAAAGGAACG 
GATGACGGCC GCATCTTTTT GGGCGGTCGC GATGGCTGCC TCTATGAAAT TTACTATCAG 
GCCGAGTCCA GTTGGTTTGG CAAGCGATGC AAGAAAATAA ATCTCTCCCA AGGACTGGTC 
TCCTATATGG TTCCCAGTTT TTTGAAGGTC TTTTCGGAAG TGGATCCAAT CGAACACATT 
GAGATCGATA ACAGTCGCAA GCTTCTCTAT GTCCTTACGG AAAAAGGTGT CATTGAGGCT 
TGGGACATCA GTACGAGTTA CACGACAGCT CGAAGACTAG GTCGGATTAC CCAGAACGAT 
ATTACGAATC AAGCAGTTAG TCTGATCACG ACTGTGGACC CGTCCATTTT TAAAAGTGTG 
AAAGCCATCT GCCCTCTGAG CGCCGACGAT GCCGACAAAC TACATCTTGT GGCCGTTACT 
CAGTGTGGTG TGCGGCTCTT TTTCTCCACA ACATCGCTAA ATGTGAAGCA ACAATTTGGT 
CCCGCGGTTC CTTGCAGTCC AGGTGAGAAT ACAGGATTCG GTCAGCCAGC AGTTCAGCCA 
CCACTGTCTC CAAATGCTGA GGCTCCTAAG GGACTTTATC TGCTCCACGT GCGTCTACCA 
CCCGGCTACA CCCCAAATGC CACCACCAAC AAACCAAAGC AGGTCCATGC CGCTCACTAC 
ACGGAGGGAA CCATGTTGAT GATCACAACG CAACAGCATG AACAGGATCT CCTCTGGTCG 
CTCAGCTCTG CCCCATCGGT TAACTTTACC TACCTGGTGG AGTCGACGGC TCTGGAGAGC 
CTGGATGGAG TTGTTTGGGG ATTGGCTGAG GTACATGAAC CGTCGACGCC ACAACGAAAG 
TCACCCTTAA ATAGTGCCCG ACACGCCCGC AAAGTAGCCC TGCTCACCAA CCAAGGCACA 
CATATCATAG AGGTGCTTAA AATGGTGGAC GTACTGCGTC AGATCCTTCT GTCGTGCAAT 
GGCCCACACC ACGAGGAGGT GAAGATGTTC TTCCAGTCGC AGAATCAGCG AGAGGCATGT 
GTCACGGCTC TGCTGCTGGC CACATCGGAT ACTTATCGTG GCAGTGACGT TGCTTTGTGG 
GCCGCCCAGG CGTTTATGCT GTACGGCGGA GAGCCATGCT ATCAGCATCA AAAGTTCCTG 
AACGCCAGCA ACCGCAATAT GGCTAATCAA ACTCTAGGCC CGAATACGAC GAATGTAAGG 
GAGCGTCAAT CCATGTTTAT GTCCACGCCA ATGCCGAATA GCGTGGCAAA TAGTCCGGTT 
GGTTTTCCTG GATCGCAGTT CAATCAGCCC ATAAGTCCAA TTGGAAATAT GCAGCCACCA 
CAGGTGGCAG TTAGTAACGA GAATTCCCCA ATCGTTTTCT CGGCCAAACA CGACGGACTT 
TACATGTATG TCTCCCGCAT GCTGCATTCT GTTTGGCAAA TGCGCTGTGT AAATGAGCAA 
TTTTGCTCTA ATCTAAGTCA GAGTGAGTGT GCCCTCTTGC TATCCGATCT GCGTTCCCTG 
CGTAGCTTTC TGGAAGTGCA CTCTGTGCAC GATATTTCCT CGACTACTCG AGTGTCATTT 
GATAACCATT TGGATAGAAC CAATTCATAT AACACCATCA TGATGGGCAA CACTTTGCTG 
CCAATTCCTG AGCAACGCGT ATTGTCCGAG CAGGCTCAAG TAGAGGAAAC ACGTTCGCTT 
TCCGCTCTCA ACCTATTTGT CAAACATGCA TGTGAGGTTA TATCGCTGTG GAACATCCTA 
AACTCGCATT CCTTCCAGCT GATATGCGTG CAACTCAGTC CGGAACATCA GAAGTTGTTG 
ACATGCTCCA CCTTCCGGGA TCTTCTGATT ACACGTTCAG AGGTCTGCGC ATTCTTGATC 
ATCTCGCTGA TCAATCTGTA TCTGAAGGAC GCGGCGGGAG TGTCTGAAGT GTCCAAGAAC 
TTACGTGAGA ACTGCCCCAA CCTATACAGG CATGAGGATG ACGTGACCTA CAAGGCCACC 
GAGTTGTTGA TGAATGCAAA GAATTGCACT TCGGCAACCG AAAAGGAACA TATGCTGCGA 
ACCACCCTGC ATATGTGCAA GGAAGCTGCT CCCACATTGC CACTGCATAG TATTTGCATG 
CAATTCATAT CGGCTGACTT CTTTGAGGGA GTGATCGAGC TTTCGGCTGT TTGTGCCTCA 
AAAAGTGATC CAGAGGAGGT TGGTGTTCAT TTCTATAACA ATGGGGAACC CGCCGATGAT 
CGCGAGGGTT ACACTTGCTT TGCCACAAGG ATGGCCTACT ACAAGGAAGT GCAACTTATG 
CTGGACCATA TCTACCAGAG AGTATGCAAC AAGACGCACG TTCAGGATAA GTCTATAAAT 
CCACTTAAAG GTACGGCTAA AGCGTCCGAT GCAAAAAACG GTGCTACGCA AACGATTCCA 
AAAATAGTGG CCCATACCCT AAAGGTGAAA GACCCGCTCA TTCATATAAC GCTCTACGAA 
TGGCTGCTGG CCCACGACAT GCTCAAAGAG CTTTTAGACG TGGTTGAGCC GTCATTAGGA 
GAGTTCTTGC GGCGCAGTGT GTCGCAAAAT GTAGATAATG TCGTTCTTAT CGATTTGCTG 
TGGAAGTACT ACGAAAAGAA TAGCCATCAT TCCCAGGCAG CCCATATATT GGACAATTTG 
GCTATGACAC GCAGTGAAAA CATTAATCTG GAGCAGCGCA TTGAGTACTT GGTGCGTGCT 
GTTATGTGTA TGCGAAATGG TAACGTTGGC TCTTCATTAA GCAATGGAAT ATTCCTGAAA 
GAGCTAGAGG ATAAGCTGGA CATCGCACGA GTTCAAAAAT CAGTGCTTGC CGCGATGACA 
GAGTTGGCTA GCGATAAGCT GGAGGCAGCT ACGGCTGTTA AGGAACTAAA CTATGCGTTG 
TACGACATAA CTCAGCTATA CCAGCACTTT GCGGAGCCCT TCGACCTTTG GGAGTGCCAA 
TTGTCCATAC TCAACTGCTC GCATCACAAC GACCCCCTAC TGATCGAGTC CGTTTGGGGC 
CAGATTATCA ACAGCGTTGT GGATAAGCCG GGCACAACTA GTGAGCGCTG CAATAGGTTA 
TTCACGAAAA TCGAAATCTT GGTAAGGGAG TATGGCGAGT CTGGAGTTTG CTTTCCCTTT 
GCCTTTCTCA TAAGGGAACT GGAAGTTAAA GCCTGCCAAC TGCGATTTCC CGAGGGCATA 
GTGCCGGAAA AACTGGTGAG TATGAATTTG GACATTGAAC TGCTCCTGGA ATACTATTCG 
AGAATGATTT CAATGAACGA ACGAGTGTGG GCCAACGAGG GTAATGAGTG GCATTTAATA 
CAATCCGTCA TACGAGTGGT CTCGCTTCTG GCAGACAACG CACAATCTAT TTGGTACCGG 
TCAAAACGAC GAATTGTTGG CAAGGCTCAA GACATAGTGG CCGGCTGCCT GAATATTTGC 
TACCAAAAGC CCGACACTAA TCGTCTTCAA CATTCACTCA AGGAACTTCA GAGTCAGCTG 
CAGCGGTTGT TGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup154 ( NM_001298898.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285827.1 Drosophila melanogaster nucleoporin 154kD, transcript variant D (Nup154), mRNA.

Price & Availability↑

CloneID	OFa16642
Clone ID Related Accession (Same CDS sequence)	NM_057939.4 , NM_001298898.1 , NM_057940.4
Accession Version	NM_001298898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin 154kD, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTCTTC CGCAAGCACA GCTGGACTTT TTCACGACGG CGACCAGTAT GCTGGAATGG 
CATTACAATC TGGAGAGAAA TAAGCCGGGT CTGCTGGAAC TGACCGGCGT TAGCCAGCAT 
GGGCGGGCCA CGATGAGCGG ACTTAATGAC TACGATTACC AGAGCCTGTC GTTTTTGAAA 
TCGGATAGAA CACATAACCT ACAACAAATG CGAACGGTGA CCAAGTCGGC TATTCCCAAC 
GAGATTCTGG AGCACTTTAA GCACATAAAA TGCCACTGCA CCATGGGCCT CTTTCCGGAA 
ATCGGACGTG CCTGGCTGAC TATCGATTCG GAAATCTACA TCTGGACGTT CAATCAGACG 
AGGGATGTGG CGTACTACGA TGGCCTCAGT CATCTGATCG TAAGCGTTGG CCTGGTGAAA 
CCAAAACCCG GCGTCTTTGT GCAGGACGTC AAGTACCTCC TGGTTCTCAC TACGCCCATT 
GAGGTCATTG TCCTGGGCGT GACATTCGGT GAGAGCTCGT ACAACGAAAT GCAGCTGATG 
AACCGGCCAG TTTTTGTGAT TGGTACCGAC AACGTGTCCA TCAGCGTCAT CAAAGGAACG 
GATGACGGCC GCATCTTTTT GGGCGGTCGC GATGGCTGCC TCTATGAAAT TTACTATCAG 
GCCGAGTCCA GTTGGTTTGG CAAGCGATGC AAGAAAATAA ATCTCTCCCA AGGACTGGTC 
TCCTATATGG TTCCCAGTTT TTTGAAGGTC TTTTCGGAAG TGGATCCAAT CGAACACATT 
GAGATCGATA ACAGTCGCAA GCTTCTCTAT GTCCTTACGG AAAAAGGTGT CATTGAGGCT 
TGGGACATCA GTACGAGTTA CACGACAGCT CGAAGACTAG GTCGGATTAC CCAGAACGAT 
ATTACGAATC AAGCAGTTAG TCTGATCACG ACTGTGGACC CGTCCATTTT TAAAAGTGTG 
AAAGCCATCT GCCCTCTGAG CGCCGACGAT GCCGACAAAC TACATCTTGT GGCCGTTACT 
CAGTGTGGTG TGCGGCTCTT TTTCTCCACA ACATCGCTAA ATGTGAAGCA ACAATTTGGT 
CCCGCGGTTC CTTGCAGTCC AGGTGAGAAT ACAGGATTCG GTCAGCCAGC AGTTCAGCCA 
CCACTGTCTC CAAATGCTGA GGCTCCTAAG GGACTTTATC TGCTCCACGT GCGTCTACCA 
CCCGGCTACA CCCCAAATGC CACCACCAAC AAACCAAAGC AGGTCCATGC CGCTCACTAC 
ACGGAGGGAA CCATGTTGAT GATCACAACG CAACAGCATG AACAGGATCT CCTCTGGTCG 
CTCAGCTCTG CCCCATCGGT TAACTTTACC TACCTGGTGG AGTCGACGGC TCTGGAGAGC 
CTGGATGGAG TTGTTTGGGG ATTGGCTGAG GTACATGAAC CGTCGACGCC ACAACGAAAG 
TCACCCTTAA ATAGTGCCCG ACACGCCCGC AAAGTAGCCC TGCTCACCAA CCAAGGCACA 
CATATCATAG AGGTGCTTAA AATGGTGGAC GTACTGCGTC AGATCCTTCT GTCGTGCAAT 
GGCCCACACC ACGAGGAGGT GAAGATGTTC TTCCAGTCGC AGAATCAGCG AGAGGCATGT 
GTCACGGCTC TGCTGCTGGC CACATCGGAT ACTTATCGTG GCAGTGACGT TGCTTTGTGG 
GCCGCCCAGG CGTTTATGCT GTACGGCGGA GAGCCATGCT ATCAGCATCA AAAGTTCCTG 
AACGCCAGCA ACCGCAATAT GGCTAATCAA ACTCTAGGCC CGAATACGAC GAATGTAAGG 
GAGCGTCAAT CCATGTTTAT GTCCACGCCA ATGCCGAATA GCGTGGCAAA TAGTCCGGTT 
GGTTTTCCTG GATCGCAGTT CAATCAGCCC ATAAGTCCAA TTGGAAATAT GCAGCCACCA 
CAGGTGGCAG TTAGTAACGA GAATTCCCCA ATCGTTTTCT CGGCCAAACA CGACGGACTT 
TACATGTATG TCTCCCGCAT GCTGCATTCT GTTTGGCAAA TGCGCTGTGT AAATGAGCAA 
TTTTGCTCTA ATCTAAGTCA GAGTGAGTGT GCCCTCTTGC TATCCGATCT GCGTTCCCTG 
CGTAGCTTTC TGGAAGTGCA CTCTGTGCAC GATATTTCCT CGACTACTCG AGTGTCATTT 
GATAACCATT TGGATAGAAC CAATTCATAT AACACCATCA TGATGGGCAA CACTTTGCTG 
CCAATTCCTG AGCAACGCGT ATTGTCCGAG CAGGCTCAAG TAGAGGAAAC ACGTTCGCTT 
TCCGCTCTCA ACCTATTTGT CAAACATGCA TGTGAGGTTA TATCGCTGTG GAACATCCTA 
AACTCGCATT CCTTCCAGCT GATATGCGTG CAACTCAGTC CGGAACATCA GAAGTTGTTG 
ACATGCTCCA CCTTCCGGGA TCTTCTGATT ACACGTTCAG AGGTCTGCGC ATTCTTGATC 
ATCTCGCTGA TCAATCTGTA TCTGAAGGAC GCGGCGGGAG TGTCTGAAGT GTCCAAGAAC 
TTACGTGAGA ACTGCCCCAA CCTATACAGG CATGAGGATG ACGTGACCTA CAAGGCCACC 
GAGTTGTTGA TGAATGCAAA GAATTGCACT TCGGCAACCG AAAAGGAACA TATGCTGCGA 
ACCACCCTGC ATATGTGCAA GGAAGCTGCT CCCACATTGC CACTGCATAG TATTTGCATG 
CAATTCATAT CGGCTGACTT CTTTGAGGGA GTGATCGAGC TTTCGGCTGT TTGTGCCTCA 
AAAAGTGATC CAGAGGAGGT TGGTGTTCAT TTCTATAACA ATGGGGAACC CGCCGATGAT 
CGCGAGGGTT ACACTTGCTT TGCCACAAGG ATGGCCTACT ACAAGGAAGT GCAACTTATG 
CTGGACCATA TCTACCAGAG AGTATGCAAC AAGACGCACG TTCAGGATAA GTCTATAAAT 
CCACTTAAAG GTACGGCTAA AGCGTCCGAT GCAAAAAACG GTGCTACGCA AACGATTCCA 
AAAATAGTGG CCCATACCCT AAAGGTGAAA GACCCGCTCA TTCATATAAC GCTCTACGAA 
TGGCTGCTGG CCCACGACAT GCTCAAAGAG CTTTTAGACG TGGTTGAGCC GTCATTAGGA 
GAGTTCTTGC GGCGCAGTGT GTCGCAAAAT GTAGATAATG TCGTTCTTAT CGATTTGCTG 
TGGAAGTACT ACGAAAAGAA TAGCCATCAT TCCCAGGCAG CCCATATATT GGACAATTTG 
GCTATGACAC GCAGTGAAAA CATTAATCTG GAGCAGCGCA TTGAGTACTT GGTGCGTGCT 
GTTATGTGTA TGCGAAATGG TAACGTTGGC TCTTCATTAA GCAATGGAAT ATTCCTGAAA 
GAGCTAGAGG ATAAGCTGGA CATCGCACGA GTTCAAAAAT CAGTGCTTGC CGCGATGACA 
GAGTTGGCTA GCGATAAGCT GGAGGCAGCT ACGGCTGTTA AGGAACTAAA CTATGCGTTG 
TACGACATAA CTCAGCTATA CCAGCACTTT GCGGAGCCCT TCGACCTTTG GGAGTGCCAA 
TTGTCCATAC TCAACTGCTC GCATCACAAC GACCCCCTAC TGATCGAGTC CGTTTGGGGC 
CAGATTATCA ACAGCGTTGT GGATAAGCCG GGCACAACTA GTGAGCGCTG CAATAGGTTA 
TTCACGAAAA TCGAAATCTT GGTAAGGGAG TATGGCGAGT CTGGAGTTTG CTTTCCCTTT 
GCCTTTCTCA TAAGGGAACT GGAAGTTAAA GCCTGCCAAC TGCGATTTCC CGAGGGCATA 
GTGCCGGAAA AACTGGTGAG TATGAATTTG GACATTGAAC TGCTCCTGGA ATACTATTCG 
AGAATGATTT CAATGAACGA ACGAGTGTGG GCCAACGAGG GTAATGAGTG GCATTTAATA 
CAATCCGTCA TACGAGTGGT CTCGCTTCTG GCAGACAACG CACAATCTAT TTGGTACCGG 
TCAAAACGAC GAATTGTTGG CAAGGCTCAA GACATAGTGG CCGGCTGCCT GAATATTTGC 
TACCAAAAGC CCGACACTAA TCGTCTTCAA CATTCACTCA AGGAACTTCA GAGTCAGCTG 
CAGCGGTTGT TGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285827.1
CDS	161..4258
Translation	MTLPQAQLDFFTTATSMLEWHYNLERNKPGLLELTGVSQHGRATMSGLNDYDYQSLSFLKSDRTHNLQQMRTVTKSAIPNEILEHFKHIKCHCTMGLFPEIGRAWLTIDSEIYIWTFNQTRDVAYYDGLSHLIVSVGLVKPKPGVFVQDVKYLLVLTTPIEVIVLGVTFGESSYNEMQLMNRPVFVIGTDNVSISVIKGTDDGRIFLGGRDGCLYEIYYQAESSWFGKRCKKINLSQGLVSYMVPSFLKVFSEVDPIEHIEIDNSRKLLYVLTEKGVIEAWDISTSYTTARRLGRITQNDITNQAVSLITTVDPSIFKSVKAICPLSADDADKLHLVAVTQCGVRLFFSTTSLNVKQQFGPAVPCSPGENTGFGQPAVQPPLSPNAEAPKGLYLLHVRLPPGYTPNATTNKPKQVHAAHYTEGTMLMITTQQHEQDLLWSLSSAPSVNFTYLVESTALESLDGVVWGLAEVHEPSTPQRKSPLNSARHARKVALLTNQGTHIIEVLKMVDVLRQILLSCNGPHHEEVKMFFQSQNQREACVTALLLATSDTYRGSDVALWAAQAFMLYGGEPCYQHQKFLNASNRNMANQTLGPNTTNVRERQSMFMSTPMPNSVANSPVGFPGSQFNQPISPIGNMQPPQVAVSNENSPIVFSAKHDGLYMYVSRMLHSVWQMRCVNEQFCSNLSQSECALLLSDLRSLRSFLEVHSVHDISSTTRVSFDNHLDRTNSYNTIMMGNTLLPIPEQRVLSEQAQVEETRSLSALNLFVKHACEVISLWNILNSHSFQLICVQLSPEHQKLLTCSTFRDLLITRSEVCAFLIISLINLYLKDAAGVSEVSKNLRENCPNLYRHEDDVTYKATELLMNAKNCTSATEKEHMLRTTLHMCKEAAPTLPLHSICMQFISADFFEGVIELSAVCASKSDPEEVGVHFYNNGEPADDREGYTCFATRMAYYKEVQLMLDHIYQRVCNKTHVQDKSINPLKGTAKASDAKNGATQTIPKIVAHTLKVKDPLIHITLYEWLLAHDMLKELLDVVEPSLGEFLRRSVSQNVDNVVLIDLLWKYYEKNSHHSQAAHILDNLAMTRSENINLEQRIEYLVRAVMCMRNGNVGSSLSNGIFLKELEDKLDIARVQKSVLAAMTELASDKLEAATAVKELNYALYDITQLYQHFAEPFDLWECQLSILNCSHHNDPLLIESVWGQIINSVVDKPGTTSERCNRLFTKIEILVREYGESGVCFPFAFLIRELEVKACQLRFPEGIVPEKLVSMNLDIELLLEYYSRMISMNERVWANEGNEWHLIQSVIRVVSLLADNAQSIWYRSKRRIVGKAQDIVAGCLNICYQKPDTNRLQHSLKELQSQLQRLLI

Target ORF information:

RefSeq Version	NM_001298898.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin 154kD, transcript variant D (Nup154), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298898.1

ORF Insert Sequence:

ATGACTCTTC CGCAAGCACA GCTGGACTTT TTCACGACGG CGACCAGTAT GCTGGAATGG 
CATTACAATC TGGAGAGAAA TAAGCCGGGT CTGCTGGAAC TGACCGGCGT TAGCCAGCAT 
GGGCGGGCCA CGATGAGCGG ACTTAATGAC TACGATTACC AGAGCCTGTC GTTTTTGAAA 
TCGGATAGAA CACATAACCT ACAACAAATG CGAACGGTGA CCAAGTCGGC TATTCCCAAC 
GAGATTCTGG AGCACTTTAA GCACATAAAA TGCCACTGCA CCATGGGCCT CTTTCCGGAA 
ATCGGACGTG CCTGGCTGAC TATCGATTCG GAAATCTACA TCTGGACGTT CAATCAGACG 
AGGGATGTGG CGTACTACGA TGGCCTCAGT CATCTGATCG TAAGCGTTGG CCTGGTGAAA 
CCAAAACCCG GCGTCTTTGT GCAGGACGTC AAGTACCTCC TGGTTCTCAC TACGCCCATT 
GAGGTCATTG TCCTGGGCGT GACATTCGGT GAGAGCTCGT ACAACGAAAT GCAGCTGATG 
AACCGGCCAG TTTTTGTGAT TGGTACCGAC AACGTGTCCA TCAGCGTCAT CAAAGGAACG 
GATGACGGCC GCATCTTTTT GGGCGGTCGC GATGGCTGCC TCTATGAAAT TTACTATCAG 
GCCGAGTCCA GTTGGTTTGG CAAGCGATGC AAGAAAATAA ATCTCTCCCA AGGACTGGTC 
TCCTATATGG TTCCCAGTTT TTTGAAGGTC TTTTCGGAAG TGGATCCAAT CGAACACATT 
GAGATCGATA ACAGTCGCAA GCTTCTCTAT GTCCTTACGG AAAAAGGTGT CATTGAGGCT 
TGGGACATCA GTACGAGTTA CACGACAGCT CGAAGACTAG GTCGGATTAC CCAGAACGAT 
ATTACGAATC AAGCAGTTAG TCTGATCACG ACTGTGGACC CGTCCATTTT TAAAAGTGTG 
AAAGCCATCT GCCCTCTGAG CGCCGACGAT GCCGACAAAC TACATCTTGT GGCCGTTACT 
CAGTGTGGTG TGCGGCTCTT TTTCTCCACA ACATCGCTAA ATGTGAAGCA ACAATTTGGT 
CCCGCGGTTC CTTGCAGTCC AGGTGAGAAT ACAGGATTCG GTCAGCCAGC AGTTCAGCCA 
CCACTGTCTC CAAATGCTGA GGCTCCTAAG GGACTTTATC TGCTCCACGT GCGTCTACCA 
CCCGGCTACA CCCCAAATGC CACCACCAAC AAACCAAAGC AGGTCCATGC CGCTCACTAC 
ACGGAGGGAA CCATGTTGAT GATCACAACG CAACAGCATG AACAGGATCT CCTCTGGTCG 
CTCAGCTCTG CCCCATCGGT TAACTTTACC TACCTGGTGG AGTCGACGGC TCTGGAGAGC 
CTGGATGGAG TTGTTTGGGG ATTGGCTGAG GTACATGAAC CGTCGACGCC ACAACGAAAG 
TCACCCTTAA ATAGTGCCCG ACACGCCCGC AAAGTAGCCC TGCTCACCAA CCAAGGCACA 
CATATCATAG AGGTGCTTAA AATGGTGGAC GTACTGCGTC AGATCCTTCT GTCGTGCAAT 
GGCCCACACC ACGAGGAGGT GAAGATGTTC TTCCAGTCGC AGAATCAGCG AGAGGCATGT 
GTCACGGCTC TGCTGCTGGC CACATCGGAT ACTTATCGTG GCAGTGACGT TGCTTTGTGG 
GCCGCCCAGG CGTTTATGCT GTACGGCGGA GAGCCATGCT ATCAGCATCA AAAGTTCCTG 
AACGCCAGCA ACCGCAATAT GGCTAATCAA ACTCTAGGCC CGAATACGAC GAATGTAAGG 
GAGCGTCAAT CCATGTTTAT GTCCACGCCA ATGCCGAATA GCGTGGCAAA TAGTCCGGTT 
GGTTTTCCTG GATCGCAGTT CAATCAGCCC ATAAGTCCAA TTGGAAATAT GCAGCCACCA 
CAGGTGGCAG TTAGTAACGA GAATTCCCCA ATCGTTTTCT CGGCCAAACA CGACGGACTT 
TACATGTATG TCTCCCGCAT GCTGCATTCT GTTTGGCAAA TGCGCTGTGT AAATGAGCAA 
TTTTGCTCTA ATCTAAGTCA GAGTGAGTGT GCCCTCTTGC TATCCGATCT GCGTTCCCTG 
CGTAGCTTTC TGGAAGTGCA CTCTGTGCAC GATATTTCCT CGACTACTCG AGTGTCATTT 
GATAACCATT TGGATAGAAC CAATTCATAT AACACCATCA TGATGGGCAA CACTTTGCTG 
CCAATTCCTG AGCAACGCGT ATTGTCCGAG CAGGCTCAAG TAGAGGAAAC ACGTTCGCTT 
TCCGCTCTCA ACCTATTTGT CAAACATGCA TGTGAGGTTA TATCGCTGTG GAACATCCTA 
AACTCGCATT CCTTCCAGCT GATATGCGTG CAACTCAGTC CGGAACATCA GAAGTTGTTG 
ACATGCTCCA CCTTCCGGGA TCTTCTGATT ACACGTTCAG AGGTCTGCGC ATTCTTGATC 
ATCTCGCTGA TCAATCTGTA TCTGAAGGAC GCGGCGGGAG TGTCTGAAGT GTCCAAGAAC 
TTACGTGAGA ACTGCCCCAA CCTATACAGG CATGAGGATG ACGTGACCTA CAAGGCCACC 
GAGTTGTTGA TGAATGCAAA GAATTGCACT TCGGCAACCG AAAAGGAACA TATGCTGCGA 
ACCACCCTGC ATATGTGCAA GGAAGCTGCT CCCACATTGC CACTGCATAG TATTTGCATG 
CAATTCATAT CGGCTGACTT CTTTGAGGGA GTGATCGAGC TTTCGGCTGT TTGTGCCTCA 
AAAAGTGATC CAGAGGAGGT TGGTGTTCAT TTCTATAACA ATGGGGAACC CGCCGATGAT 
CGCGAGGGTT ACACTTGCTT TGCCACAAGG ATGGCCTACT ACAAGGAAGT GCAACTTATG 
CTGGACCATA TCTACCAGAG AGTATGCAAC AAGACGCACG TTCAGGATAA GTCTATAAAT 
CCACTTAAAG GTACGGCTAA AGCGTCCGAT GCAAAAAACG GTGCTACGCA AACGATTCCA 
AAAATAGTGG CCCATACCCT AAAGGTGAAA GACCCGCTCA TTCATATAAC GCTCTACGAA 
TGGCTGCTGG CCCACGACAT GCTCAAAGAG CTTTTAGACG TGGTTGAGCC GTCATTAGGA 
GAGTTCTTGC GGCGCAGTGT GTCGCAAAAT GTAGATAATG TCGTTCTTAT CGATTTGCTG 
TGGAAGTACT ACGAAAAGAA TAGCCATCAT TCCCAGGCAG CCCATATATT GGACAATTTG 
GCTATGACAC GCAGTGAAAA CATTAATCTG GAGCAGCGCA TTGAGTACTT GGTGCGTGCT 
GTTATGTGTA TGCGAAATGG TAACGTTGGC TCTTCATTAA GCAATGGAAT ATTCCTGAAA 
GAGCTAGAGG ATAAGCTGGA CATCGCACGA GTTCAAAAAT CAGTGCTTGC CGCGATGACA 
GAGTTGGCTA GCGATAAGCT GGAGGCAGCT ACGGCTGTTA AGGAACTAAA CTATGCGTTG 
TACGACATAA CTCAGCTATA CCAGCACTTT GCGGAGCCCT TCGACCTTTG GGAGTGCCAA 
TTGTCCATAC TCAACTGCTC GCATCACAAC GACCCCCTAC TGATCGAGTC CGTTTGGGGC 
CAGATTATCA ACAGCGTTGT GGATAAGCCG GGCACAACTA GTGAGCGCTG CAATAGGTTA 
TTCACGAAAA TCGAAATCTT GGTAAGGGAG TATGGCGAGT CTGGAGTTTG CTTTCCCTTT 
GCCTTTCTCA TAAGGGAACT GGAAGTTAAA GCCTGCCAAC TGCGATTTCC CGAGGGCATA 
GTGCCGGAAA AACTGGTGAG TATGAATTTG GACATTGAAC TGCTCCTGGA ATACTATTCG 
AGAATGATTT CAATGAACGA ACGAGTGTGG GCCAACGAGG GTAATGAGTG GCATTTAATA 
CAATCCGTCA TACGAGTGGT CTCGCTTCTG GCAGACAACG CACAATCTAT TTGGTACCGG 
TCAAAACGAC GAATTGTTGG CAAGGCTCAA GACATAGTGG CCGGCTGCCT GAATATTTGC 
TACCAAAAGC CCGACACTAA TCGTCTTCAA CATTCACTCA AGGAACTTCA GAGTCAGCTG 
CAGCGGTTGT TGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nup154 ( NM_057940.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477288.3 Drosophila melanogaster nucleoporin 154kD, transcript variant C (Nup154), mRNA.

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CloneID	OFa16642
Clone ID Related Accession (Same CDS sequence)	NM_057939.4 , NM_001298898.1 , NM_057940.4
Accession Version	NM_057940.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleoporin 154kD, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057940.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTCTTC CGCAAGCACA GCTGGACTTT TTCACGACGG CGACCAGTAT GCTGGAATGG 
CATTACAATC TGGAGAGAAA TAAGCCGGGT CTGCTGGAAC TGACCGGCGT TAGCCAGCAT 
GGGCGGGCCA CGATGAGCGG ACTTAATGAC TACGATTACC AGAGCCTGTC GTTTTTGAAA 
TCGGATAGAA CACATAACCT ACAACAAATG CGAACGGTGA CCAAGTCGGC TATTCCCAAC 
GAGATTCTGG AGCACTTTAA GCACATAAAA TGCCACTGCA CCATGGGCCT CTTTCCGGAA 
ATCGGACGTG CCTGGCTGAC TATCGATTCG GAAATCTACA TCTGGACGTT CAATCAGACG 
AGGGATGTGG CGTACTACGA TGGCCTCAGT CATCTGATCG TAAGCGTTGG CCTGGTGAAA 
CCAAAACCCG GCGTCTTTGT GCAGGACGTC AAGTACCTCC TGGTTCTCAC TACGCCCATT 
GAGGTCATTG TCCTGGGCGT GACATTCGGT GAGAGCTCGT ACAACGAAAT GCAGCTGATG 
AACCGGCCAG TTTTTGTGAT TGGTACCGAC AACGTGTCCA TCAGCGTCAT CAAAGGAACG 
GATGACGGCC GCATCTTTTT GGGCGGTCGC GATGGCTGCC TCTATGAAAT TTACTATCAG 
GCCGAGTCCA GTTGGTTTGG CAAGCGATGC AAGAAAATAA ATCTCTCCCA AGGACTGGTC 
TCCTATATGG TTCCCAGTTT TTTGAAGGTC TTTTCGGAAG TGGATCCAAT CGAACACATT 
GAGATCGATA ACAGTCGCAA GCTTCTCTAT GTCCTTACGG AAAAAGGTGT CATTGAGGCT 
TGGGACATCA GTACGAGTTA CACGACAGCT CGAAGACTAG GTCGGATTAC CCAGAACGAT 
ATTACGAATC AAGCAGTTAG TCTGATCACG ACTGTGGACC CGTCCATTTT TAAAAGTGTG 
AAAGCCATCT GCCCTCTGAG CGCCGACGAT GCCGACAAAC TACATCTTGT GGCCGTTACT 
CAGTGTGGTG TGCGGCTCTT TTTCTCCACA ACATCGCTAA ATGTGAAGCA ACAATTTGGT 
CCCGCGGTTC CTTGCAGTCC AGGTGAGAAT ACAGGATTCG GTCAGCCAGC AGTTCAGCCA 
CCACTGTCTC CAAATGCTGA GGCTCCTAAG GGACTTTATC TGCTCCACGT GCGTCTACCA 
CCCGGCTACA CCCCAAATGC CACCACCAAC AAACCAAAGC AGGTCCATGC CGCTCACTAC 
ACGGAGGGAA CCATGTTGAT GATCACAACG CAACAGCATG AACAGGATCT CCTCTGGTCG 
CTCAGCTCTG CCCCATCGGT TAACTTTACC TACCTGGTGG AGTCGACGGC TCTGGAGAGC 
CTGGATGGAG TTGTTTGGGG ATTGGCTGAG GTACATGAAC CGTCGACGCC ACAACGAAAG 
TCACCCTTAA ATAGTGCCCG ACACGCCCGC AAAGTAGCCC TGCTCACCAA CCAAGGCACA 
CATATCATAG AGGTGCTTAA AATGGTGGAC GTACTGCGTC AGATCCTTCT GTCGTGCAAT 
GGCCCACACC ACGAGGAGGT GAAGATGTTC TTCCAGTCGC AGAATCAGCG AGAGGCATGT 
GTCACGGCTC TGCTGCTGGC CACATCGGAT ACTTATCGTG GCAGTGACGT TGCTTTGTGG 
GCCGCCCAGG CGTTTATGCT GTACGGCGGA GAGCCATGCT ATCAGCATCA AAAGTTCCTG 
AACGCCAGCA ACCGCAATAT GGCTAATCAA ACTCTAGGCC CGAATACGAC GAATGTAAGG 
GAGCGTCAAT CCATGTTTAT GTCCACGCCA ATGCCGAATA GCGTGGCAAA TAGTCCGGTT 
GGTTTTCCTG GATCGCAGTT CAATCAGCCC ATAAGTCCAA TTGGAAATAT GCAGCCACCA 
CAGGTGGCAG TTAGTAACGA GAATTCCCCA ATCGTTTTCT CGGCCAAACA CGACGGACTT 
TACATGTATG TCTCCCGCAT GCTGCATTCT GTTTGGCAAA TGCGCTGTGT AAATGAGCAA 
TTTTGCTCTA ATCTAAGTCA GAGTGAGTGT GCCCTCTTGC TATCCGATCT GCGTTCCCTG 
CGTAGCTTTC TGGAAGTGCA CTCTGTGCAC GATATTTCCT CGACTACTCG AGTGTCATTT 
GATAACCATT TGGATAGAAC CAATTCATAT AACACCATCA TGATGGGCAA CACTTTGCTG 
CCAATTCCTG AGCAACGCGT ATTGTCCGAG CAGGCTCAAG TAGAGGAAAC ACGTTCGCTT 
TCCGCTCTCA ACCTATTTGT CAAACATGCA TGTGAGGTTA TATCGCTGTG GAACATCCTA 
AACTCGCATT CCTTCCAGCT GATATGCGTG CAACTCAGTC CGGAACATCA GAAGTTGTTG 
ACATGCTCCA CCTTCCGGGA TCTTCTGATT ACACGTTCAG AGGTCTGCGC ATTCTTGATC 
ATCTCGCTGA TCAATCTGTA TCTGAAGGAC GCGGCGGGAG TGTCTGAAGT GTCCAAGAAC 
TTACGTGAGA ACTGCCCCAA CCTATACAGG CATGAGGATG ACGTGACCTA CAAGGCCACC 
GAGTTGTTGA TGAATGCAAA GAATTGCACT TCGGCAACCG AAAAGGAACA TATGCTGCGA 
ACCACCCTGC ATATGTGCAA GGAAGCTGCT CCCACATTGC CACTGCATAG TATTTGCATG 
CAATTCATAT CGGCTGACTT CTTTGAGGGA GTGATCGAGC TTTCGGCTGT TTGTGCCTCA 
AAAAGTGATC CAGAGGAGGT TGGTGTTCAT TTCTATAACA ATGGGGAACC CGCCGATGAT 
CGCGAGGGTT ACACTTGCTT TGCCACAAGG ATGGCCTACT ACAAGGAAGT GCAACTTATG 
CTGGACCATA TCTACCAGAG AGTATGCAAC AAGACGCACG TTCAGGATAA GTCTATAAAT 
CCACTTAAAG GTACGGCTAA AGCGTCCGAT GCAAAAAACG GTGCTACGCA AACGATTCCA 
AAAATAGTGG CCCATACCCT AAAGGTGAAA GACCCGCTCA TTCATATAAC GCTCTACGAA 
TGGCTGCTGG CCCACGACAT GCTCAAAGAG CTTTTAGACG TGGTTGAGCC GTCATTAGGA 
GAGTTCTTGC GGCGCAGTGT GTCGCAAAAT GTAGATAATG TCGTTCTTAT CGATTTGCTG 
TGGAAGTACT ACGAAAAGAA TAGCCATCAT TCCCAGGCAG CCCATATATT GGACAATTTG 
GCTATGACAC GCAGTGAAAA CATTAATCTG GAGCAGCGCA TTGAGTACTT GGTGCGTGCT 
GTTATGTGTA TGCGAAATGG TAACGTTGGC TCTTCATTAA GCAATGGAAT ATTCCTGAAA 
GAGCTAGAGG ATAAGCTGGA CATCGCACGA GTTCAAAAAT CAGTGCTTGC CGCGATGACA 
GAGTTGGCTA GCGATAAGCT GGAGGCAGCT ACGGCTGTTA AGGAACTAAA CTATGCGTTG 
TACGACATAA CTCAGCTATA CCAGCACTTT GCGGAGCCCT TCGACCTTTG GGAGTGCCAA 
TTGTCCATAC TCAACTGCTC GCATCACAAC GACCCCCTAC TGATCGAGTC CGTTTGGGGC 
CAGATTATCA ACAGCGTTGT GGATAAGCCG GGCACAACTA GTGAGCGCTG CAATAGGTTA 
TTCACGAAAA TCGAAATCTT GGTAAGGGAG TATGGCGAGT CTGGAGTTTG CTTTCCCTTT 
GCCTTTCTCA TAAGGGAACT GGAAGTTAAA GCCTGCCAAC TGCGATTTCC CGAGGGCATA 
GTGCCGGAAA AACTGGTGAG TATGAATTTG GACATTGAAC TGCTCCTGGA ATACTATTCG 
AGAATGATTT CAATGAACGA ACGAGTGTGG GCCAACGAGG GTAATGAGTG GCATTTAATA 
CAATCCGTCA TACGAGTGGT CTCGCTTCTG GCAGACAACG CACAATCTAT TTGGTACCGG 
TCAAAACGAC GAATTGTTGG CAAGGCTCAA GACATAGTGG CCGGCTGCCT GAATATTTGC 
TACCAAAAGC CCGACACTAA TCGTCTTCAA CATTCACTCA AGGAACTTCA GAGTCAGCTG 
CAGCGGTTGT TGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477288.3
CDS	161..4258
Translation	MTLPQAQLDFFTTATSMLEWHYNLERNKPGLLELTGVSQHGRATMSGLNDYDYQSLSFLKSDRTHNLQQMRTVTKSAIPNEILEHFKHIKCHCTMGLFPEIGRAWLTIDSEIYIWTFNQTRDVAYYDGLSHLIVSVGLVKPKPGVFVQDVKYLLVLTTPIEVIVLGVTFGESSYNEMQLMNRPVFVIGTDNVSISVIKGTDDGRIFLGGRDGCLYEIYYQAESSWFGKRCKKINLSQGLVSYMVPSFLKVFSEVDPIEHIEIDNSRKLLYVLTEKGVIEAWDISTSYTTARRLGRITQNDITNQAVSLITTVDPSIFKSVKAICPLSADDADKLHLVAVTQCGVRLFFSTTSLNVKQQFGPAVPCSPGENTGFGQPAVQPPLSPNAEAPKGLYLLHVRLPPGYTPNATTNKPKQVHAAHYTEGTMLMITTQQHEQDLLWSLSSAPSVNFTYLVESTALESLDGVVWGLAEVHEPSTPQRKSPLNSARHARKVALLTNQGTHIIEVLKMVDVLRQILLSCNGPHHEEVKMFFQSQNQREACVTALLLATSDTYRGSDVALWAAQAFMLYGGEPCYQHQKFLNASNRNMANQTLGPNTTNVRERQSMFMSTPMPNSVANSPVGFPGSQFNQPISPIGNMQPPQVAVSNENSPIVFSAKHDGLYMYVSRMLHSVWQMRCVNEQFCSNLSQSECALLLSDLRSLRSFLEVHSVHDISSTTRVSFDNHLDRTNSYNTIMMGNTLLPIPEQRVLSEQAQVEETRSLSALNLFVKHACEVISLWNILNSHSFQLICVQLSPEHQKLLTCSTFRDLLITRSEVCAFLIISLINLYLKDAAGVSEVSKNLRENCPNLYRHEDDVTYKATELLMNAKNCTSATEKEHMLRTTLHMCKEAAPTLPLHSICMQFISADFFEGVIELSAVCASKSDPEEVGVHFYNNGEPADDREGYTCFATRMAYYKEVQLMLDHIYQRVCNKTHVQDKSINPLKGTAKASDAKNGATQTIPKIVAHTLKVKDPLIHITLYEWLLAHDMLKELLDVVEPSLGEFLRRSVSQNVDNVVLIDLLWKYYEKNSHHSQAAHILDNLAMTRSENINLEQRIEYLVRAVMCMRNGNVGSSLSNGIFLKELEDKLDIARVQKSVLAAMTELASDKLEAATAVKELNYALYDITQLYQHFAEPFDLWECQLSILNCSHHNDPLLIESVWGQIINSVVDKPGTTSERCNRLFTKIEILVREYGESGVCFPFAFLIRELEVKACQLRFPEGIVPEKLVSMNLDIELLLEYYSRMISMNERVWANEGNEWHLIQSVIRVVSLLADNAQSIWYRSKRRIVGKAQDIVAGCLNICYQKPDTNRLQHSLKELQSQLQRLLI

Target ORF information:

RefSeq Version	NM_057940.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleoporin 154kD, transcript variant C (Nup154), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057940.4

ORF Insert Sequence:

ATGACTCTTC CGCAAGCACA GCTGGACTTT TTCACGACGG CGACCAGTAT GCTGGAATGG 
CATTACAATC TGGAGAGAAA TAAGCCGGGT CTGCTGGAAC TGACCGGCGT TAGCCAGCAT 
GGGCGGGCCA CGATGAGCGG ACTTAATGAC TACGATTACC AGAGCCTGTC GTTTTTGAAA 
TCGGATAGAA CACATAACCT ACAACAAATG CGAACGGTGA CCAAGTCGGC TATTCCCAAC 
GAGATTCTGG AGCACTTTAA GCACATAAAA TGCCACTGCA CCATGGGCCT CTTTCCGGAA 
ATCGGACGTG CCTGGCTGAC TATCGATTCG GAAATCTACA TCTGGACGTT CAATCAGACG 
AGGGATGTGG CGTACTACGA TGGCCTCAGT CATCTGATCG TAAGCGTTGG CCTGGTGAAA 
CCAAAACCCG GCGTCTTTGT GCAGGACGTC AAGTACCTCC TGGTTCTCAC TACGCCCATT 
GAGGTCATTG TCCTGGGCGT GACATTCGGT GAGAGCTCGT ACAACGAAAT GCAGCTGATG 
AACCGGCCAG TTTTTGTGAT TGGTACCGAC AACGTGTCCA TCAGCGTCAT CAAAGGAACG 
GATGACGGCC GCATCTTTTT GGGCGGTCGC GATGGCTGCC TCTATGAAAT TTACTATCAG 
GCCGAGTCCA GTTGGTTTGG CAAGCGATGC AAGAAAATAA ATCTCTCCCA AGGACTGGTC 
TCCTATATGG TTCCCAGTTT TTTGAAGGTC TTTTCGGAAG TGGATCCAAT CGAACACATT 
GAGATCGATA ACAGTCGCAA GCTTCTCTAT GTCCTTACGG AAAAAGGTGT CATTGAGGCT 
TGGGACATCA GTACGAGTTA CACGACAGCT CGAAGACTAG GTCGGATTAC CCAGAACGAT 
ATTACGAATC AAGCAGTTAG TCTGATCACG ACTGTGGACC CGTCCATTTT TAAAAGTGTG 
AAAGCCATCT GCCCTCTGAG CGCCGACGAT GCCGACAAAC TACATCTTGT GGCCGTTACT 
CAGTGTGGTG TGCGGCTCTT TTTCTCCACA ACATCGCTAA ATGTGAAGCA ACAATTTGGT 
CCCGCGGTTC CTTGCAGTCC AGGTGAGAAT ACAGGATTCG GTCAGCCAGC AGTTCAGCCA 
CCACTGTCTC CAAATGCTGA GGCTCCTAAG GGACTTTATC TGCTCCACGT GCGTCTACCA 
CCCGGCTACA CCCCAAATGC CACCACCAAC AAACCAAAGC AGGTCCATGC CGCTCACTAC 
ACGGAGGGAA CCATGTTGAT GATCACAACG CAACAGCATG AACAGGATCT CCTCTGGTCG 
CTCAGCTCTG CCCCATCGGT TAACTTTACC TACCTGGTGG AGTCGACGGC TCTGGAGAGC 
CTGGATGGAG TTGTTTGGGG ATTGGCTGAG GTACATGAAC CGTCGACGCC ACAACGAAAG 
TCACCCTTAA ATAGTGCCCG ACACGCCCGC AAAGTAGCCC TGCTCACCAA CCAAGGCACA 
CATATCATAG AGGTGCTTAA AATGGTGGAC GTACTGCGTC AGATCCTTCT GTCGTGCAAT 
GGCCCACACC ACGAGGAGGT GAAGATGTTC TTCCAGTCGC AGAATCAGCG AGAGGCATGT 
GTCACGGCTC TGCTGCTGGC CACATCGGAT ACTTATCGTG GCAGTGACGT TGCTTTGTGG 
GCCGCCCAGG CGTTTATGCT GTACGGCGGA GAGCCATGCT ATCAGCATCA AAAGTTCCTG 
AACGCCAGCA ACCGCAATAT GGCTAATCAA ACTCTAGGCC CGAATACGAC GAATGTAAGG 
GAGCGTCAAT CCATGTTTAT GTCCACGCCA ATGCCGAATA GCGTGGCAAA TAGTCCGGTT 
GGTTTTCCTG GATCGCAGTT CAATCAGCCC ATAAGTCCAA TTGGAAATAT GCAGCCACCA 
CAGGTGGCAG TTAGTAACGA GAATTCCCCA ATCGTTTTCT CGGCCAAACA CGACGGACTT 
TACATGTATG TCTCCCGCAT GCTGCATTCT GTTTGGCAAA TGCGCTGTGT AAATGAGCAA 
TTTTGCTCTA ATCTAAGTCA GAGTGAGTGT GCCCTCTTGC TATCCGATCT GCGTTCCCTG 
CGTAGCTTTC TGGAAGTGCA CTCTGTGCAC GATATTTCCT CGACTACTCG AGTGTCATTT 
GATAACCATT TGGATAGAAC CAATTCATAT AACACCATCA TGATGGGCAA CACTTTGCTG 
CCAATTCCTG AGCAACGCGT ATTGTCCGAG CAGGCTCAAG TAGAGGAAAC ACGTTCGCTT 
TCCGCTCTCA ACCTATTTGT CAAACATGCA TGTGAGGTTA TATCGCTGTG GAACATCCTA 
AACTCGCATT CCTTCCAGCT GATATGCGTG CAACTCAGTC CGGAACATCA GAAGTTGTTG 
ACATGCTCCA CCTTCCGGGA TCTTCTGATT ACACGTTCAG AGGTCTGCGC ATTCTTGATC 
ATCTCGCTGA TCAATCTGTA TCTGAAGGAC GCGGCGGGAG TGTCTGAAGT GTCCAAGAAC 
TTACGTGAGA ACTGCCCCAA CCTATACAGG CATGAGGATG ACGTGACCTA CAAGGCCACC 
GAGTTGTTGA TGAATGCAAA GAATTGCACT TCGGCAACCG AAAAGGAACA TATGCTGCGA 
ACCACCCTGC ATATGTGCAA GGAAGCTGCT CCCACATTGC CACTGCATAG TATTTGCATG 
CAATTCATAT CGGCTGACTT CTTTGAGGGA GTGATCGAGC TTTCGGCTGT TTGTGCCTCA 
AAAAGTGATC CAGAGGAGGT TGGTGTTCAT TTCTATAACA ATGGGGAACC CGCCGATGAT 
CGCGAGGGTT ACACTTGCTT TGCCACAAGG ATGGCCTACT ACAAGGAAGT GCAACTTATG 
CTGGACCATA TCTACCAGAG AGTATGCAAC AAGACGCACG TTCAGGATAA GTCTATAAAT 
CCACTTAAAG GTACGGCTAA AGCGTCCGAT GCAAAAAACG GTGCTACGCA AACGATTCCA 
AAAATAGTGG CCCATACCCT AAAGGTGAAA GACCCGCTCA TTCATATAAC GCTCTACGAA 
TGGCTGCTGG CCCACGACAT GCTCAAAGAG CTTTTAGACG TGGTTGAGCC GTCATTAGGA 
GAGTTCTTGC GGCGCAGTGT GTCGCAAAAT GTAGATAATG TCGTTCTTAT CGATTTGCTG 
TGGAAGTACT ACGAAAAGAA TAGCCATCAT TCCCAGGCAG CCCATATATT GGACAATTTG 
GCTATGACAC GCAGTGAAAA CATTAATCTG GAGCAGCGCA TTGAGTACTT GGTGCGTGCT 
GTTATGTGTA TGCGAAATGG TAACGTTGGC TCTTCATTAA GCAATGGAAT ATTCCTGAAA 
GAGCTAGAGG ATAAGCTGGA CATCGCACGA GTTCAAAAAT CAGTGCTTGC CGCGATGACA 
GAGTTGGCTA GCGATAAGCT GGAGGCAGCT ACGGCTGTTA AGGAACTAAA CTATGCGTTG 
TACGACATAA CTCAGCTATA CCAGCACTTT GCGGAGCCCT TCGACCTTTG GGAGTGCCAA 
TTGTCCATAC TCAACTGCTC GCATCACAAC GACCCCCTAC TGATCGAGTC CGTTTGGGGC 
CAGATTATCA ACAGCGTTGT GGATAAGCCG GGCACAACTA GTGAGCGCTG CAATAGGTTA 
TTCACGAAAA TCGAAATCTT GGTAAGGGAG TATGGCGAGT CTGGAGTTTG CTTTCCCTTT 
GCCTTTCTCA TAAGGGAACT GGAAGTTAAA GCCTGCCAAC TGCGATTTCC CGAGGGCATA 
GTGCCGGAAA AACTGGTGAG TATGAATTTG GACATTGAAC TGCTCCTGGA ATACTATTCG 
AGAATGATTT CAATGAACGA ACGAGTGTGG GCCAACGAGG GTAATGAGTG GCATTTAATA 
CAATCCGTCA TACGAGTGGT CTCGCTTCTG GCAGACAACG CACAATCTAT TTGGTACCGG 
TCAAAACGAC GAATTGTTGG CAAGGCTCAA GACATAGTGG CCGGCTGCCT GAATATTTGC 
TACCAAAAGC CCGACACTAA TCGTCTTCAA CATTCACTCA AGGAACTTCA GAGTCAGCTG 
CAGCGGTTGT TGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nup154 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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