piwi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	piwi
Entrez Gene ID	34521
Full Name	P-element induced wimpy testis
Synonyms	CG6122,Dmel\CG6122,PIWI,Piwi
General protein information	Preferred Names P-element induced wimpy testis Names CG6122 gene product from transcript CG6122-RA CG6122-PA CG6122-PB piwi-PA piwi-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32C1-32C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_057527.4	NP_476875.1	P-element induced wimpy testis, isoform A
NM_001298896.1	NP_001285825.1	P-element induced wimpy testis, isoform B

Pathways from BioSystems

Homologies

	PIWIL3	XP_001099093.1
Canis lupus familiaris (dog)	LOC486307	XP_005636578.1
Homo sapiens (human)	PIWIL3	NP_001242904.1
Pan troglodytes (chimpanzee)	PIWIL3	XP_003317193.1
Drosophila melanogaster (fruit fly)	piwi	NP_476875.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report the mapping of multiple Piwi-interacting chromosomal domains in somatic cells of ovaries. These domains significantly overlap with genomic regions tethered to Nuclear Pore Complexes.
Title: Piwi interacts with chromatin at nuclear pores and promiscuously binds nuclear transcripts in Drosophila ovarian somatic cells.

The identity of piRNA clusters is epigenetically acquired in a Piwi-dependent manner during embryonic development.
Title: Piwi Is Required during Drosophila Embryogenesis to License Dual-Strand piRNA Clusters for Transposon Repression in Adult Ovaries.

Analysis of piRNA clusters containing remnants of I-elements shows increased expression of the piRNA (PIWI-interacting RNA )precursors and enrichment by the Heterochromatin Protein 1 homolog, Rhino, in weak R strains, which is in accordance with stronger piRNA expression by these regions
Title: Natural variation of piRNA expression affects immunity to transposable elements.

Data show that Cbp80 knockdown not only led to the upregulation of transposons, but also to delocalization of Piwi, Aub and Ago3, key factors in the piRNA biosynthesis pathway.
Title: Cbp80 is needed for the expression of piRNA components and piRNAs.

Piwi represses c-Fos in the somatic niche to regulate germ stem cell maintenance and differentiation and in the somatic follicle cells to affect morphogenesis.
Title: c-Fos Repression by Piwi Regulates Drosophila Ovarian Germline Formation and Tissue Morphogenesis.

The following piwi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the piwi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16674	NM_001298896.1 Latest version!	Drosophila melanogaster P-element induced wimpy testis (piwi), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16674	NM_057527.4 Latest version!	Drosophila melanogaster P-element induced wimpy testis (piwi), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

piwi ( NM_057527.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476875.1 Drosophila melanogaster P-element induced wimpy testis (piwi), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16674
Clone ID Related Accession (Same CDS sequence)	NM_057527.4 , NM_001298896.1
Accession Version	NM_057527.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	P-element induced wimpy testis, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057527.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGATG ATCAGGGACG TGGACGCAGG CGTCCACTTA ACGAAGATGA TTCCTCTACT 
TCCCGAGGTA GTGGTGATGG GCCGCGGGTG AAAGTATTCA GAGGATCTTC ATCAGGTGAC 
CCGAGAGCGG ATCCTCGTAT AGAGGCTTCA AGAGAGAGAA GAGCTCTCGA GGAAGCTCCC 
AGGCGTGAAG GTGGCCCGAC AGAGCGAAAG CCGTGGGGTG ACCAATATGA TTACCTGAAT 
ACCCGTCCGG CTGAGCTGGT ATCCAAGAAG GGAACCGATG GCGTCCCGGT CATGCTGCAG 
ACGAACTTTT TCCGATTAAA AACCAAGCCG GAATGGCGGA TCGTTCATTA TCACGTGGAG 
TTTGAGCCGA GCATCGAGAA TCCTCGTGTC CGTATGGGAG TTTTGTCCAA TCATGCTAAC 
CTTCTGGGAT CAGGCTATCT ATTCGATGGA CTGCAACTGT TCACCACCAG GAAATTCGAG 
CAGGAAATCA CGGTGCTCAG CGGAAAGTCG AAGCTGGACA TTGAATACAA GATATCCATA 
AAGTTCGTTG GATTCATATC GTGTGCTGAG CCCCGCTTTT TGCAAGTCTT AAATCTAATA 
TTGCGCCGCT CCATGAAGGG CCTAAATTTG GAATTAGTTG GCCGTAATCT CTTTGATCCC 
CGAGCTAAGA TCGAAATAAG GGAGTTCAAA ATGGAGCTAT GGCCGGGCTA TGAGACATCG 
ATTCGTCAGC ACGAAAAAGA TATTTTATTG GGCACCGAAA TAACTCACAA AGTTATGCGC 
ACCGAGACGA TCTACGACAT AATGCGACGT TGCTCACACA ATCCGGCTCG TCATCAGGAC 
GAAGTACGGG TAAATGTTTT GGACTTGATT GTCCTTACGG ATTACAATAA CAGAACTTAT 
CGTATCAATG ATGTCGACTT TGGACAAACT CCGAAATCAA CATTCAGTTG CAAGGGTAGA 
GATATCAGTT TCGTGGAATA CTATCTCACT AAATATAATA TACGCATTCG CGACCACAAT 
CAGCCGCTGC TGATCTCCAA AAATAGGGAC AAGGCTCTAA AAACTAACGC TAGCGAATTA 
GTGGTACTAA TTCCTGAGCT CTGCCGAGTG ACTGGGCTCA ATGCCGAGAT GCGCTCAAAC 
TTTCAGCTTA TGCGTGCCAT GAGCAGTTAT ACGCGAATGA ACCCCAAACA ACGCACTGAT 
CGATTGCGCG CTTTTAACCA CCGTTTACAA AACACTCCAG AAAGTGTGAA GGTCTTGAGA 
GACTGGAACA TGGAACTGGA CAAGAACGTC ACAGAAGTAC AAGGCCGGAT AATTGGACAG 
CAGAACATCG TGTTTCATAA TGGAAAGGTT CCTGCTGGAG AAAACGCTGA TTGGCAAAGG 
CACTTCAGAG ACCAAAGGAT GCTTACCACT CCGAGCGATG GCCTCGATCG TTGGGCTGTC 
ATCGCGCCGC AAAGGAATTC CCATGAACTC CGAACTCTAC TTGACTCTTT GTATAGAGCA 
GCTAGTGGAA TGGGTCTTAG AATTCGAAGC CCCCAGGAAT TCATAATTTA TGATGATCGC 
ACTGGAACTT ATGTGAGAGC AATGGATGAT TGTGTGCGCT CAGATCCCAA ACTTATATTA 
TGCCTCGTAC CCAATGATAA CGCCGAAAGA TACTCATCAA TCAAAAAGAG AGGATACGTT 
GACAGGGCGG TGCCAACTCA AGTTGTGACC CTTAAAACGA CCAAGAACCG TAGCCTTATG 
AGCATTGCCA CCAAAATAGC AATCCAACTG AATTGCAAGT TGGGATATAC ACCCTGGATG 
ATCGAACTAC CCTTGTCCGG ACTGATGACA ATTGGCTTTG ACATTGCGAA GAGCACACGA 
GATCGGAAGA GGGCCTACGG AGCATTGATT GCCTCAATGG ATCTACAGCA AAACTCCACG 
TACTTCAGCA CAGTCACGGA GTGCAGCGCC TTTGATGTGC TCGCTAACAC CCTTTGGCCG 
ATGATAGCAA AGGCCCTGCG CCAATATCAA CATGAGCATA GGAAGCTGCC ATCTCGAATC 
GTATTTTATC GAGACGGTGT GAGCTCCGGC TCTCTAAAGC AGCTTTTTGA ATTTGAAGTC 
AAGGACATCA TTGAGAAGTT GAAAACTGAA TACGCCCGCG TCCAGCTAAG CCCACCGCAA 
TTAGCTTATA TTGTGGTAAC CAGATCCATG AACACGCGCT TCTTCCTCAA CGGACAAAAT 
CCTCCGCCTG GTACTATAGT TGATGACGTT ATAACTCTGC CCGAGAGATA CGACTTTTAT 
CTGGTCTCGC AACAAGTTCG TCAGGGTACA GTGTCGCCGA CCAGCTACAA TGTTCTTTAT 
AGCAGCATGG GTCTCTCACC GGAGAAAATG CAAAAACTTA CGTACAAGAT GTGCCACTTG 
TACTACAATT GGTCGGGCAC CACACGAGTG CCAGCAGTTT GCCAGTACGC TAAGAAGCTA 
GCTACCCTCG TGGGTACGAA CTTGCACTCT ATTCCGCAAA ACGCGCTCGA AAAGAAGTTT 
TATTATCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476875.1
CDS	89..2620
Translation	MADDQGRGRRRPLNEDDSSTSRGSGDGPRVKVFRGSSSGDPRADPRIEASRERRALEEAPRREGGPTERKPWGDQYDYLNTRPAELVSKKGTDGVPVMLQTNFFRLKTKPEWRIVHYHVEFEPSIENPRVRMGVLSNHANLLGSGYLFDGLQLFTTRKFEQEITVLSGKSKLDIEYKISIKFVGFISCAEPRFLQVLNLILRRSMKGLNLELVGRNLFDPRAKIEIREFKMELWPGYETSIRQHEKDILLGTEITHKVMRTETIYDIMRRCSHNPARHQDEVRVNVLDLIVLTDYNNRTYRINDVDFGQTPKSTFSCKGRDISFVEYYLTKYNIRIRDHNQPLLISKNRDKALKTNASELVVLIPELCRVTGLNAEMRSNFQLMRAMSSYTRMNPKQRTDRLRAFNHRLQNTPESVKVLRDWNMELDKNVTEVQGRIIGQQNIVFHNGKVPAGENADWQRHFRDQRMLTTPSDGLDRWAVIAPQRNSHELRTLLDSLYRAASGMGLRIRSPQEFIIYDDRTGTYVRAMDDCVRSDPKLILCLVPNDNAERYSSIKKRGYVDRAVPTQVVTLKTTKNRSLMSIATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDRKRAYGALIASMDLQQNSTYFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQLFEFEVKDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNGQNPPPGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYNVLYSSMGLSPEKMQKLTYKMCHLYYNWSGTTRVPAVCQYAKKLATLVGTNLHSIPQNALEKKFYYL

Target ORF information:

RefSeq Version	NM_057527.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster P-element induced wimpy testis (piwi), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057527.4

ORF Insert Sequence:

ATGGCTGATG ATCAGGGACG TGGACGCAGG CGTCCACTTA ACGAAGATGA TTCCTCTACT 
TCCCGAGGTA GTGGTGATGG GCCGCGGGTG AAAGTATTCA GAGGATCTTC ATCAGGTGAC 
CCGAGAGCGG ATCCTCGTAT AGAGGCTTCA AGAGAGAGAA GAGCTCTCGA GGAAGCTCCC 
AGGCGTGAAG GTGGCCCGAC AGAGCGAAAG CCGTGGGGTG ACCAATATGA TTACCTGAAT 
ACCCGTCCGG CTGAGCTGGT ATCCAAGAAG GGAACCGATG GCGTCCCGGT CATGCTGCAG 
ACGAACTTTT TCCGATTAAA AACCAAGCCG GAATGGCGGA TCGTTCATTA TCACGTGGAG 
TTTGAGCCGA GCATCGAGAA TCCTCGTGTC CGTATGGGAG TTTTGTCCAA TCATGCTAAC 
CTTCTGGGAT CAGGCTATCT ATTCGATGGA CTGCAACTGT TCACCACCAG GAAATTCGAG 
CAGGAAATCA CGGTGCTCAG CGGAAAGTCG AAGCTGGACA TTGAATACAA GATATCCATA 
AAGTTCGTTG GATTCATATC GTGTGCTGAG CCCCGCTTTT TGCAAGTCTT AAATCTAATA 
TTGCGCCGCT CCATGAAGGG CCTAAATTTG GAATTAGTTG GCCGTAATCT CTTTGATCCC 
CGAGCTAAGA TCGAAATAAG GGAGTTCAAA ATGGAGCTAT GGCCGGGCTA TGAGACATCG 
ATTCGTCAGC ACGAAAAAGA TATTTTATTG GGCACCGAAA TAACTCACAA AGTTATGCGC 
ACCGAGACGA TCTACGACAT AATGCGACGT TGCTCACACA ATCCGGCTCG TCATCAGGAC 
GAAGTACGGG TAAATGTTTT GGACTTGATT GTCCTTACGG ATTACAATAA CAGAACTTAT 
CGTATCAATG ATGTCGACTT TGGACAAACT CCGAAATCAA CATTCAGTTG CAAGGGTAGA 
GATATCAGTT TCGTGGAATA CTATCTCACT AAATATAATA TACGCATTCG CGACCACAAT 
CAGCCGCTGC TGATCTCCAA AAATAGGGAC AAGGCTCTAA AAACTAACGC TAGCGAATTA 
GTGGTACTAA TTCCTGAGCT CTGCCGAGTG ACTGGGCTCA ATGCCGAGAT GCGCTCAAAC 
TTTCAGCTTA TGCGTGCCAT GAGCAGTTAT ACGCGAATGA ACCCCAAACA ACGCACTGAT 
CGATTGCGCG CTTTTAACCA CCGTTTACAA AACACTCCAG AAAGTGTGAA GGTCTTGAGA 
GACTGGAACA TGGAACTGGA CAAGAACGTC ACAGAAGTAC AAGGCCGGAT AATTGGACAG 
CAGAACATCG TGTTTCATAA TGGAAAGGTT CCTGCTGGAG AAAACGCTGA TTGGCAAAGG 
CACTTCAGAG ACCAAAGGAT GCTTACCACT CCGAGCGATG GCCTCGATCG TTGGGCTGTC 
ATCGCGCCGC AAAGGAATTC CCATGAACTC CGAACTCTAC TTGACTCTTT GTATAGAGCA 
GCTAGTGGAA TGGGTCTTAG AATTCGAAGC CCCCAGGAAT TCATAATTTA TGATGATCGC 
ACTGGAACTT ATGTGAGAGC AATGGATGAT TGTGTGCGCT CAGATCCCAA ACTTATATTA 
TGCCTCGTAC CCAATGATAA CGCCGAAAGA TACTCATCAA TCAAAAAGAG AGGATACGTT 
GACAGGGCGG TGCCAACTCA AGTTGTGACC CTTAAAACGA CCAAGAACCG TAGCCTTATG 
AGCATTGCCA CCAAAATAGC AATCCAACTG AATTGCAAGT TGGGATATAC ACCCTGGATG 
ATCGAACTAC CCTTGTCCGG ACTGATGACA ATTGGCTTTG ACATTGCGAA GAGCACACGA 
GATCGGAAGA GGGCCTACGG AGCATTGATT GCCTCAATGG ATCTACAGCA AAACTCCACG 
TACTTCAGCA CAGTCACGGA GTGCAGCGCC TTTGATGTGC TCGCTAACAC CCTTTGGCCG 
ATGATAGCAA AGGCCCTGCG CCAATATCAA CATGAGCATA GGAAGCTGCC ATCTCGAATC 
GTATTTTATC GAGACGGTGT GAGCTCCGGC TCTCTAAAGC AGCTTTTTGA ATTTGAAGTC 
AAGGACATCA TTGAGAAGTT GAAAACTGAA TACGCCCGCG TCCAGCTAAG CCCACCGCAA 
TTAGCTTATA TTGTGGTAAC CAGATCCATG AACACGCGCT TCTTCCTCAA CGGACAAAAT 
CCTCCGCCTG GTACTATAGT TGATGACGTT ATAACTCTGC CCGAGAGATA CGACTTTTAT 
CTGGTCTCGC AACAAGTTCG TCAGGGTACA GTGTCGCCGA CCAGCTACAA TGTTCTTTAT 
AGCAGCATGG GTCTCTCACC GGAGAAAATG CAAAAACTTA CGTACAAGAT GTGCCACTTG 
TACTACAATT GGTCGGGCAC CACACGAGTG CCAGCAGTTT GCCAGTACGC TAAGAAGCTA 
GCTACCCTCG TGGGTACGAA CTTGCACTCT ATTCCGCAAA ACGCGCTCGA AAAGAAGTTT 
TATTATCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

piwi ( NM_001298896.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285825.1 Drosophila melanogaster P-element induced wimpy testis (piwi), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16674
Clone ID Related Accession (Same CDS sequence)	NM_057527.4 , NM_001298896.1
Accession Version	NM_001298896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	P-element induced wimpy testis, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGATG ATCAGGGACG TGGACGCAGG CGTCCACTTA ACGAAGATGA TTCCTCTACT 
TCCCGAGGTA GTGGTGATGG GCCGCGGGTG AAAGTATTCA GAGGATCTTC ATCAGGTGAC 
CCGAGAGCGG ATCCTCGTAT AGAGGCTTCA AGAGAGAGAA GAGCTCTCGA GGAAGCTCCC 
AGGCGTGAAG GTGGCCCGAC AGAGCGAAAG CCGTGGGGTG ACCAATATGA TTACCTGAAT 
ACCCGTCCGG CTGAGCTGGT ATCCAAGAAG GGAACCGATG GCGTCCCGGT CATGCTGCAG 
ACGAACTTTT TCCGATTAAA AACCAAGCCG GAATGGCGGA TCGTTCATTA TCACGTGGAG 
TTTGAGCCGA GCATCGAGAA TCCTCGTGTC CGTATGGGAG TTTTGTCCAA TCATGCTAAC 
CTTCTGGGAT CAGGCTATCT ATTCGATGGA CTGCAACTGT TCACCACCAG GAAATTCGAG 
CAGGAAATCA CGGTGCTCAG CGGAAAGTCG AAGCTGGACA TTGAATACAA GATATCCATA 
AAGTTCGTTG GATTCATATC GTGTGCTGAG CCCCGCTTTT TGCAAGTCTT AAATCTAATA 
TTGCGCCGCT CCATGAAGGG CCTAAATTTG GAATTAGTTG GCCGTAATCT CTTTGATCCC 
CGAGCTAAGA TCGAAATAAG GGAGTTCAAA ATGGAGCTAT GGCCGGGCTA TGAGACATCG 
ATTCGTCAGC ACGAAAAAGA TATTTTATTG GGCACCGAAA TAACTCACAA AGTTATGCGC 
ACCGAGACGA TCTACGACAT AATGCGACGT TGCTCACACA ATCCGGCTCG TCATCAGGAC 
GAAGTACGGG TAAATGTTTT GGACTTGATT GTCCTTACGG ATTACAATAA CAGAACTTAT 
CGTATCAATG ATGTCGACTT TGGACAAACT CCGAAATCAA CATTCAGTTG CAAGGGTAGA 
GATATCAGTT TCGTGGAATA CTATCTCACT AAATATAATA TACGCATTCG CGACCACAAT 
CAGCCGCTGC TGATCTCCAA AAATAGGGAC AAGGCTCTAA AAACTAACGC TAGCGAATTA 
GTGGTACTAA TTCCTGAGCT CTGCCGAGTG ACTGGGCTCA ATGCCGAGAT GCGCTCAAAC 
TTTCAGCTTA TGCGTGCCAT GAGCAGTTAT ACGCGAATGA ACCCCAAACA ACGCACTGAT 
CGATTGCGCG CTTTTAACCA CCGTTTACAA AACACTCCAG AAAGTGTGAA GGTCTTGAGA 
GACTGGAACA TGGAACTGGA CAAGAACGTC ACAGAAGTAC AAGGCCGGAT AATTGGACAG 
CAGAACATCG TGTTTCATAA TGGAAAGGTT CCTGCTGGAG AAAACGCTGA TTGGCAAAGG 
CACTTCAGAG ACCAAAGGAT GCTTACCACT CCGAGCGATG GCCTCGATCG TTGGGCTGTC 
ATCGCGCCGC AAAGGAATTC CCATGAACTC CGAACTCTAC TTGACTCTTT GTATAGAGCA 
GCTAGTGGAA TGGGTCTTAG AATTCGAAGC CCCCAGGAAT TCATAATTTA TGATGATCGC 
ACTGGAACTT ATGTGAGAGC AATGGATGAT TGTGTGCGCT CAGATCCCAA ACTTATATTA 
TGCCTCGTAC CCAATGATAA CGCCGAAAGA TACTCATCAA TCAAAAAGAG AGGATACGTT 
GACAGGGCGG TGCCAACTCA AGTTGTGACC CTTAAAACGA CCAAGAACCG TAGCCTTATG 
AGCATTGCCA CCAAAATAGC AATCCAACTG AATTGCAAGT TGGGATATAC ACCCTGGATG 
ATCGAACTAC CCTTGTCCGG ACTGATGACA ATTGGCTTTG ACATTGCGAA GAGCACACGA 
GATCGGAAGA GGGCCTACGG AGCATTGATT GCCTCAATGG ATCTACAGCA AAACTCCACG 
TACTTCAGCA CAGTCACGGA GTGCAGCGCC TTTGATGTGC TCGCTAACAC CCTTTGGCCG 
ATGATAGCAA AGGCCCTGCG CCAATATCAA CATGAGCATA GGAAGCTGCC ATCTCGAATC 
GTATTTTATC GAGACGGTGT GAGCTCCGGC TCTCTAAAGC AGCTTTTTGA ATTTGAAGTC 
AAGGACATCA TTGAGAAGTT GAAAACTGAA TACGCCCGCG TCCAGCTAAG CCCACCGCAA 
TTAGCTTATA TTGTGGTAAC CAGATCCATG AACACGCGCT TCTTCCTCAA CGGACAAAAT 
CCTCCGCCTG GTACTATAGT TGATGACGTT ATAACTCTGC CCGAGAGATA CGACTTTTAT 
CTGGTCTCGC AACAAGTTCG TCAGGGTACA GTGTCGCCGA CCAGCTACAA TGTTCTTTAT 
AGCAGCATGG GTCTCTCACC GGAGAAAATG CAAAAACTTA CGTACAAGAT GTGCCACTTG 
TACTACAATT GGTCGGGCAC CACACGAGTG CCAGCAGTTT GCCAGTACGC TAAGAAGCTA 
GCTACCCTCG TGGGTACGAA CTTGCACTCT ATTCCGCAAA ACGCGCTCGA AAAGAAGTTT 
TATTATCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285825.1
CDS	89..2620
Translation	MADDQGRGRRRPLNEDDSSTSRGSGDGPRVKVFRGSSSGDPRADPRIEASRERRALEEAPRREGGPTERKPWGDQYDYLNTRPAELVSKKGTDGVPVMLQTNFFRLKTKPEWRIVHYHVEFEPSIENPRVRMGVLSNHANLLGSGYLFDGLQLFTTRKFEQEITVLSGKSKLDIEYKISIKFVGFISCAEPRFLQVLNLILRRSMKGLNLELVGRNLFDPRAKIEIREFKMELWPGYETSIRQHEKDILLGTEITHKVMRTETIYDIMRRCSHNPARHQDEVRVNVLDLIVLTDYNNRTYRINDVDFGQTPKSTFSCKGRDISFVEYYLTKYNIRIRDHNQPLLISKNRDKALKTNASELVVLIPELCRVTGLNAEMRSNFQLMRAMSSYTRMNPKQRTDRLRAFNHRLQNTPESVKVLRDWNMELDKNVTEVQGRIIGQQNIVFHNGKVPAGENADWQRHFRDQRMLTTPSDGLDRWAVIAPQRNSHELRTLLDSLYRAASGMGLRIRSPQEFIIYDDRTGTYVRAMDDCVRSDPKLILCLVPNDNAERYSSIKKRGYVDRAVPTQVVTLKTTKNRSLMSIATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDRKRAYGALIASMDLQQNSTYFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQLFEFEVKDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNGQNPPPGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYNVLYSSMGLSPEKMQKLTYKMCHLYYNWSGTTRVPAVCQYAKKLATLVGTNLHSIPQNALEKKFYYL

Target ORF information:

RefSeq Version	NM_001298896.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster P-element induced wimpy testis (piwi), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298896.1

ORF Insert Sequence:

ATGGCTGATG ATCAGGGACG TGGACGCAGG CGTCCACTTA ACGAAGATGA TTCCTCTACT 
TCCCGAGGTA GTGGTGATGG GCCGCGGGTG AAAGTATTCA GAGGATCTTC ATCAGGTGAC 
CCGAGAGCGG ATCCTCGTAT AGAGGCTTCA AGAGAGAGAA GAGCTCTCGA GGAAGCTCCC 
AGGCGTGAAG GTGGCCCGAC AGAGCGAAAG CCGTGGGGTG ACCAATATGA TTACCTGAAT 
ACCCGTCCGG CTGAGCTGGT ATCCAAGAAG GGAACCGATG GCGTCCCGGT CATGCTGCAG 
ACGAACTTTT TCCGATTAAA AACCAAGCCG GAATGGCGGA TCGTTCATTA TCACGTGGAG 
TTTGAGCCGA GCATCGAGAA TCCTCGTGTC CGTATGGGAG TTTTGTCCAA TCATGCTAAC 
CTTCTGGGAT CAGGCTATCT ATTCGATGGA CTGCAACTGT TCACCACCAG GAAATTCGAG 
CAGGAAATCA CGGTGCTCAG CGGAAAGTCG AAGCTGGACA TTGAATACAA GATATCCATA 
AAGTTCGTTG GATTCATATC GTGTGCTGAG CCCCGCTTTT TGCAAGTCTT AAATCTAATA 
TTGCGCCGCT CCATGAAGGG CCTAAATTTG GAATTAGTTG GCCGTAATCT CTTTGATCCC 
CGAGCTAAGA TCGAAATAAG GGAGTTCAAA ATGGAGCTAT GGCCGGGCTA TGAGACATCG 
ATTCGTCAGC ACGAAAAAGA TATTTTATTG GGCACCGAAA TAACTCACAA AGTTATGCGC 
ACCGAGACGA TCTACGACAT AATGCGACGT TGCTCACACA ATCCGGCTCG TCATCAGGAC 
GAAGTACGGG TAAATGTTTT GGACTTGATT GTCCTTACGG ATTACAATAA CAGAACTTAT 
CGTATCAATG ATGTCGACTT TGGACAAACT CCGAAATCAA CATTCAGTTG CAAGGGTAGA 
GATATCAGTT TCGTGGAATA CTATCTCACT AAATATAATA TACGCATTCG CGACCACAAT 
CAGCCGCTGC TGATCTCCAA AAATAGGGAC AAGGCTCTAA AAACTAACGC TAGCGAATTA 
GTGGTACTAA TTCCTGAGCT CTGCCGAGTG ACTGGGCTCA ATGCCGAGAT GCGCTCAAAC 
TTTCAGCTTA TGCGTGCCAT GAGCAGTTAT ACGCGAATGA ACCCCAAACA ACGCACTGAT 
CGATTGCGCG CTTTTAACCA CCGTTTACAA AACACTCCAG AAAGTGTGAA GGTCTTGAGA 
GACTGGAACA TGGAACTGGA CAAGAACGTC ACAGAAGTAC AAGGCCGGAT AATTGGACAG 
CAGAACATCG TGTTTCATAA TGGAAAGGTT CCTGCTGGAG AAAACGCTGA TTGGCAAAGG 
CACTTCAGAG ACCAAAGGAT GCTTACCACT CCGAGCGATG GCCTCGATCG TTGGGCTGTC 
ATCGCGCCGC AAAGGAATTC CCATGAACTC CGAACTCTAC TTGACTCTTT GTATAGAGCA 
GCTAGTGGAA TGGGTCTTAG AATTCGAAGC CCCCAGGAAT TCATAATTTA TGATGATCGC 
ACTGGAACTT ATGTGAGAGC AATGGATGAT TGTGTGCGCT CAGATCCCAA ACTTATATTA 
TGCCTCGTAC CCAATGATAA CGCCGAAAGA TACTCATCAA TCAAAAAGAG AGGATACGTT 
GACAGGGCGG TGCCAACTCA AGTTGTGACC CTTAAAACGA CCAAGAACCG TAGCCTTATG 
AGCATTGCCA CCAAAATAGC AATCCAACTG AATTGCAAGT TGGGATATAC ACCCTGGATG 
ATCGAACTAC CCTTGTCCGG ACTGATGACA ATTGGCTTTG ACATTGCGAA GAGCACACGA 
GATCGGAAGA GGGCCTACGG AGCATTGATT GCCTCAATGG ATCTACAGCA AAACTCCACG 
TACTTCAGCA CAGTCACGGA GTGCAGCGCC TTTGATGTGC TCGCTAACAC CCTTTGGCCG 
ATGATAGCAA AGGCCCTGCG CCAATATCAA CATGAGCATA GGAAGCTGCC ATCTCGAATC 
GTATTTTATC GAGACGGTGT GAGCTCCGGC TCTCTAAAGC AGCTTTTTGA ATTTGAAGTC 
AAGGACATCA TTGAGAAGTT GAAAACTGAA TACGCCCGCG TCCAGCTAAG CCCACCGCAA 
TTAGCTTATA TTGTGGTAAC CAGATCCATG AACACGCGCT TCTTCCTCAA CGGACAAAAT 
CCTCCGCCTG GTACTATAGT TGATGACGTT ATAACTCTGC CCGAGAGATA CGACTTTTAT 
CTGGTCTCGC AACAAGTTCG TCAGGGTACA GTGTCGCCGA CCAGCTACAA TGTTCTTTAT 
AGCAGCATGG GTCTCTCACC GGAGAAAATG CAAAAACTTA CGTACAAGAT GTGCCACTTG 
TACTACAATT GGTCGGGCAC CACACGAGTG CCAGCAGTTT GCCAGTACGC TAAGAAGCTA 
GCTACCCTCG TGGGTACGAA CTTGCACTCT ATTCCGCAAA ACGCGCTCGA AAAGAAGTTT 
TATTATCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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piwi cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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