Stam cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Stam
Entrez Gene ID	34505
Full Name	Signal transducing adaptor molecule
Synonyms	CG6521,Dmel\CG6521,Dstam,STAM,Stam1,dstam,stam
General protein information	Preferred Names Signal transducing adaptor molecule Names CG6521 gene product from transcript CG6521-RA CG6521-PA Stam-PA signal transducer adaptor molecule signal transducing adaptor molecule signal-transducing adaptor molecule
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32B2-32B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_058100.4	NP_477448.1	signal transducing adaptor molecule

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Stam2	NP_001012085.1
Homo sapiens (human)	STAM2	NP_005834.4
Pan troglodytes (chimpanzee)	STAM2	XP_515834.2
	STAM2	XP_002808053.1
Bos taurus (cattle)	STAM2	NP_001069574.1
Mus musculus (house mouse)	Stam2	NP_062641.1
Canis lupus familiaris (dog)	STAM2	XP_005632050.1
Gallus gallus (chicken)	STAM2	NP_001012940.1
Drosophila melanogaster (fruit fly)	Stam	NP_477448.1
Xenopus tropicalis (tropical clawed frog)	stam2	XP_002936702.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results reveal that ESCRT-0 (ESCRT-0 components stam and hrs)mutants inhibit EGFR signaling by disrupting Rhomboid endosomal trafficking in the ligand-producing cells.
Title: ESCRT-0 complex modulates Rbf-mutant cell survival by regulating Rhomboid endosomal trafficking and EGFR signaling.

2 Drosophila epithelia lacking Stam, or Hrs and Stam, accumulate Notch and Dome in endosomes, maintain normal apico-basal polarity and proliferation control and do not show ectopic Notch signaling activation.
Title: ESCRT-0 is not required for ectopic Notch activation and tumor suppression in Drosophila.

analysis of dSTAM expression pattern during wild type and mutant egg chamber development in D. melanogaster
Title: dSTAM expression pattern during wild type and mutant egg chamber development in D. melanogaster.

The following Stam gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Stam cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14427	NM_058100.4 Latest version!	Drosophila melanogaster signal transducing adaptor molecule (Stam), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Stam ( NM_058100.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477448.1 Drosophila melanogaster signal transducing adaptor molecule (Stam), mRNA.

Price & Availability↑

CloneID	OFa14427
Clone ID Related Accession (Same CDS sequence)	NM_058100.4
Accession Version	NM_058100.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	signal transducing adaptor molecule
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_058100.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAATCT TTGGGCAGTC GTCGCCATTC GACGCCGACG TGGAAAAGGC CACCAGCGAA 
ACTAATACCA ACGACAACTG GTCTCTGATT TTGGATGTAT GCGACAAGGT AACGACCAAT 
CCCCGCCTGG CCAAGGATTG CCTGAAGGCG GTGATGCGCC GCATGGGCCA CACCGATCCT 
CATGTGGTGA TGCAGGCGAT TACGCTTTTG GACGCCCTGT CCAACAACTG CGGAAAACCG 
CTCCATTTGG AGGTGGCTTC ACGGGATTTC GAGACGGAGT TCCGCCGCCT GTTGGCCAAA 
GCACAGCCAA AGGTTTCACT GAAAATGCGC CAAGTGCTGA AGAACTGGGC TGAAAACGAC 
TATAAGAACG ATCGAGAGCT CGACTTGATA CCCGCACTCT ATGCCAAGCT GCGCCAAGAG 
GGATATGATT TCAAGAATCT TGGCGATAAG ACTAGCAAGA CTGTTGCCGA AAAGGCCGCC 
GCCCTGCCCA AAGATCCGAA TGTGGTCAGC AGCCAGCAGG AAGAGGATGA CATCGCCAAG 
GCCATCGAAT TGTCGCTGAA GGAGAACAAG GGCAGTCCAA AGACTGGGAG TGTAGCAAGT 
ACCGGCACCG CCAGTGCCTA TCCCTCATTG TATCCATCTT TCGCTGGAAC CGGCTCGAGT 
GCACCGAGCG CCGAAGCCAG TTCCGCACCA GCGCCAGAGC CGAGAAAGGT TCGAGCTCTG 
TACGATTTCG AAGCTGCCGA GGAAAACGAA TTGACCTTCT TTGCCGGCGA GATCATTCAT 
GTGCTGGATG ATAGTGATCC AAACTGGTGG AAGGGATACA ATCAACGCGG CGAGGGTCTC 
TTCCCCTCGA ACTTTGTCAC TGCCGATCTG TCCGTCGATC CCGAGCGGCT GGACATCAAT 
CAGCAGCATA AGTCGGCCGC CGCAGCCGGG CAACGAGAGT TGGATTCCGC TGCGGCACTG 
CAGCAAAAGA CTGAAGCGGC AGCAGCTGCC GCCGCAGCTC AGCCTGTGGA GATCGACGAG 
TCAAAGATCG ACCGGCTGCT GCATTTGCTG CACGAGGCGA ATCCCGAAGA TCCCTCGCAG 
GACAGCGACG AAATGCTGCA GCTGGAGCAG GAGGTCCATC AGATGGGACC GCTGATCGAT 
GCCGAGCTGG AACGCGTCGA CCGCAAGCAC GCTCAACTGA CGCAGTTGTC CAGCGACCTC 
GTCGACGCCA TTAATCTGTA CCATACCTTG ATGCGGGATG ACCGTGCTGC GGCTGGACAT 
TTTGCCGCCG CTGCTGGCGG CTTTATGCCT GGACTTGCTG GTTTCCCGGG TGCCGCTCTG 
TACGGTGCTC CAGGTTATCC TGCAGCGGGT GGATTCCCAC CGGCCCAAAA TTATCCGGGC 
ATGCACTCGA TGCCATATGG CCCCGTACCA AATCCTCCCA CTTCTGCTCC ATCTCAGGCA 
CCTGGAGCTC CTGGTGGCTA TCTGGGTCAA CCGCAGGGGC AGGTGCCACT GCCCTATCAG 
AACGGACATG CCGCACAGAT GAACTCGCTG CCAGCCCATT TGACTCAACC CACTGGCACT 
GCACCCATGC CCCAGCCAAT CTACACTCAG CCGACTCCCG TGACCACCCA ACAGCAGCAG 
CAGCCACTTC CACAGCACCC TCAACAGCCG CCACAGCAGC AAGGCCAGCA GCCCCCACAG 
CATCCCCAGA ACAGCTACCA CATCGACCCC CAGTCACAGT ACGCCCAAGT TCCGCCCGCA 
GTCACACAAA TCCAGCAGCC GCAGCAGCCA CCGGCTCCGC ACGCTTACAT GTCGCCGCAG 
CATCAGCAGG CACCGCCACC ACAGGGATAC CAGCCAGGAC CAAATCTTTA TGGACCGAAC 
GGATCGCCGG CCGTCGTCAA CCCGCAGAGC TACATGTCCC CACAGCAGCA GCAGCAACAA 
CACCCACCAC CACACGATCA GTTTAGTCAG CTGCATCAGC AGATGGCCGC CATGTCCATG 
GCTGGCGGAC CGCCAGTTGG GGCGCCCGGC TATCACATGC AGAACGATGC TGTCAAGAAC 
AACATTCCTA TCTACCAGCA GCAGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477448.1
CDS	300..2369
Translation	MGIFGQSSPFDADVEKATSETNTNDNWSLILDVCDKVTTNPRLAKDCLKAVMRRMGHTDPHVVMQAITLLDALSNNCGKPLHLEVASRDFETEFRRLLAKAQPKVSLKMRQVLKNWAENDYKNDRELDLIPALYAKLRQEGYDFKNLGDKTSKTVAEKAAALPKDPNVVSSQQEEDDIAKAIELSLKENKGSPKTGSVASTGTASAYPSLYPSFAGTGSSAPSAEASSAPAPEPRKVRALYDFEAAEENELTFFAGEIIHVLDDSDPNWWKGYNQRGEGLFPSNFVTADLSVDPERLDINQQHKSAAAAGQRELDSAAALQQKTEAAAAAAAAQPVEIDESKIDRLLHLLHEANPEDPSQDSDEMLQLEQEVHQMGPLIDAELERVDRKHAQLTQLSSDLVDAINLYHTLMRDDRAAAGHFAAAAGGFMPGLAGFPGAALYGAPGYPAAGGFPPAQNYPGMHSMPYGPVPNPPTSAPSQAPGAPGGYLGQPQGQVPLPYQNGHAAQMNSLPAHLTQPTGTAPMPQPIYTQPTPVTTQQQQQPLPQHPQQPPQQQGQQPPQHPQNSYHIDPQSQYAQVPPAVTQIQQPQQPPAPHAYMSPQHQQAPPPQGYQPGPNLYGPNGSPAVVNPQSYMSPQQQQQQHPPPHDQFSQLHQQMAAMSMAGGPPVGAPGYHMQNDAVKNNIPIYQQQR

Target ORF information:

RefSeq Version	NM_058100.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster signal transducing adaptor molecule (Stam), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058100.4

ORF Insert Sequence:

ATGGGAATCT TTGGGCAGTC GTCGCCATTC GACGCCGACG TGGAAAAGGC CACCAGCGAA 
ACTAATACCA ACGACAACTG GTCTCTGATT TTGGATGTAT GCGACAAGGT AACGACCAAT 
CCCCGCCTGG CCAAGGATTG CCTGAAGGCG GTGATGCGCC GCATGGGCCA CACCGATCCT 
CATGTGGTGA TGCAGGCGAT TACGCTTTTG GACGCCCTGT CCAACAACTG CGGAAAACCG 
CTCCATTTGG AGGTGGCTTC ACGGGATTTC GAGACGGAGT TCCGCCGCCT GTTGGCCAAA 
GCACAGCCAA AGGTTTCACT GAAAATGCGC CAAGTGCTGA AGAACTGGGC TGAAAACGAC 
TATAAGAACG ATCGAGAGCT CGACTTGATA CCCGCACTCT ATGCCAAGCT GCGCCAAGAG 
GGATATGATT TCAAGAATCT TGGCGATAAG ACTAGCAAGA CTGTTGCCGA AAAGGCCGCC 
GCCCTGCCCA AAGATCCGAA TGTGGTCAGC AGCCAGCAGG AAGAGGATGA CATCGCCAAG 
GCCATCGAAT TGTCGCTGAA GGAGAACAAG GGCAGTCCAA AGACTGGGAG TGTAGCAAGT 
ACCGGCACCG CCAGTGCCTA TCCCTCATTG TATCCATCTT TCGCTGGAAC CGGCTCGAGT 
GCACCGAGCG CCGAAGCCAG TTCCGCACCA GCGCCAGAGC CGAGAAAGGT TCGAGCTCTG 
TACGATTTCG AAGCTGCCGA GGAAAACGAA TTGACCTTCT TTGCCGGCGA GATCATTCAT 
GTGCTGGATG ATAGTGATCC AAACTGGTGG AAGGGATACA ATCAACGCGG CGAGGGTCTC 
TTCCCCTCGA ACTTTGTCAC TGCCGATCTG TCCGTCGATC CCGAGCGGCT GGACATCAAT 
CAGCAGCATA AGTCGGCCGC CGCAGCCGGG CAACGAGAGT TGGATTCCGC TGCGGCACTG 
CAGCAAAAGA CTGAAGCGGC AGCAGCTGCC GCCGCAGCTC AGCCTGTGGA GATCGACGAG 
TCAAAGATCG ACCGGCTGCT GCATTTGCTG CACGAGGCGA ATCCCGAAGA TCCCTCGCAG 
GACAGCGACG AAATGCTGCA GCTGGAGCAG GAGGTCCATC AGATGGGACC GCTGATCGAT 
GCCGAGCTGG AACGCGTCGA CCGCAAGCAC GCTCAACTGA CGCAGTTGTC CAGCGACCTC 
GTCGACGCCA TTAATCTGTA CCATACCTTG ATGCGGGATG ACCGTGCTGC GGCTGGACAT 
TTTGCCGCCG CTGCTGGCGG CTTTATGCCT GGACTTGCTG GTTTCCCGGG TGCCGCTCTG 
TACGGTGCTC CAGGTTATCC TGCAGCGGGT GGATTCCCAC CGGCCCAAAA TTATCCGGGC 
ATGCACTCGA TGCCATATGG CCCCGTACCA AATCCTCCCA CTTCTGCTCC ATCTCAGGCA 
CCTGGAGCTC CTGGTGGCTA TCTGGGTCAA CCGCAGGGGC AGGTGCCACT GCCCTATCAG 
AACGGACATG CCGCACAGAT GAACTCGCTG CCAGCCCATT TGACTCAACC CACTGGCACT 
GCACCCATGC CCCAGCCAAT CTACACTCAG CCGACTCCCG TGACCACCCA ACAGCAGCAG 
CAGCCACTTC CACAGCACCC TCAACAGCCG CCACAGCAGC AAGGCCAGCA GCCCCCACAG 
CATCCCCAGA ACAGCTACCA CATCGACCCC CAGTCACAGT ACGCCCAAGT TCCGCCCGCA 
GTCACACAAA TCCAGCAGCC GCAGCAGCCA CCGGCTCCGC ACGCTTACAT GTCGCCGCAG 
CATCAGCAGG CACCGCCACC ACAGGGATAC CAGCCAGGAC CAAATCTTTA TGGACCGAAC 
GGATCGCCGG CCGTCGTCAA CCCGCAGAGC TACATGTCCC CACAGCAGCA GCAGCAACAA 
CACCCACCAC CACACGATCA GTTTAGTCAG CTGCATCAGC AGATGGCCGC CATGTCCATG 
GCTGGCGGAC CGCCAGTTGG GGCGCCCGGC TATCACATGC AGAACGATGC TGTCAAGAAC 
AACATTCCTA TCTACCAGCA GCAGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Stam cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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