CG12299 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG12299
Entrez Gene ID	34483
Full Name	CG12299 gene product from transcript CG12299-RA
Synonyms	Dmel\CG12299
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32A5-32A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_135604.3	NP_609448.1	uncharacterized protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC101906388	XP_005219650.1
Drosophila melanogaster (fruit fly)	CG12299	NP_609448.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG12299 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG12299 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14403	NM_135604.3 Latest version!	Drosophila melanogaster uncharacterized protein (CG12299), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG12299 ( NM_135604.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609448.1 Drosophila melanogaster uncharacterized protein (CG12299), mRNA.

Price & Availability↑

CloneID	OFa14403
Clone ID Related Accession (Same CDS sequence)	NM_135604.3
Accession Version	NM_135604.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 31, 2011 this sequence version replaced NM_135604.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGTGC AGCTGAAACA GAGCTACCAC CACCTGCCCC TGACGATCAC GCCAATCCTG 
CAGCAGACGC ACCTCCTGCC GCAACAGGCA CCCAATCCAC CCCAGCAGCA ACCGCAGCAG 
CCACAGCCGC CGGATCTCAC CTTCCACTGC ATGTGCTGTG CGGAGTTCTT CGTCCATCCG 
CTGGCGCTGT ACCAGCACAT GAACACCCTG CACCCCCATG AGCCGGGCAA TGGACAGCAA 
GAGCAGGAGA GCCCGGGCGA CGAGAGCGAG GACTACAGCT GGATATTCGA GCCGGTGTGC 
GAGCTGGCGG AGGATGGCAG CGACTCGTCG GATGGGAGCG CCTCCTCGGG CTCCGACAGC 
TCCTCGTCGT CGGACGACGA CGATGACGAT GACGACGACG ACAGCAGCAG CTCCAGTTCG 
AGTTCCAGCA ACTCGAGCTC ATCAAGCAGC TCGGTGCCGA CCACCAGCAA CTCCAATACC 
CAGCAGAGCC AGGAGTCAGT GCAGCCACTT CACGGCCTGG TTGCCGGTCC GGGCTACAAT 
GAGTTCCAGC TGCAGATGAC AGATCCGCGG GAGTCCACCT CCATATTCAT GGTGCAGCCG 
ACCGTAAGCG TCACGCCCTT GCAGCAGCTA CTACCACCGG CACCCACTGT TAGCCCGGGA 
TTGGGCCTGC AATCGACGCC CATCAAGCGG CGGCGCGGAA GGCGGAGCAA CATCGGTGCA 
CCCGTGATGG ATCCAGCGTT GAATGGCAAC CAGAAATGCT TCCAGTGCAC GCACTGCGAG 
GCGAGCTTTC CCAACGCCGG CGATCTGTCC AAGCACGTGC GCTCGCACAT CACCAACAAG 
CCGTTCCAGT GCTCCATCTG CCAGAAGACA TTCACGCACA TCGGCAGCCT GAACACGCAC 
ATCCGCATCC ACAGCGGCGA GAAGCCGTAC AAGTGCGAGC TCTGCCCGAA GGCCTTCACG 
CAGTCCAGCA GCCTGATGGT GCACATGCGC TCGCATTCGG TGCGCAAGCC GCATCAGTGT 
GTGCAGTGCG ACAAGGGATT CATCAACTAC AGCTCGCTGC TGCTGCACCA GAAGACGCAC 
ATCGCGCCCA CGGAGACGTT CATCTGTCCG GAGTGCGAGC GCGAATTCAA GGCGGAGGCG 
CTGCTCGACG AGCACATGCG CATGCATACG CAGGAGCTGG TGTACCAGTG CGCCATCTGC 
CGCGAGGCCT TCCGCGCCAG CTCGGAGCTG GTACAGCACA TGAAGAACCA CATGGGCGAG 
AAGCCATTCA CCTGCTCGCT GTGCGACCGC TCGTTCACGC AGTCGGGTAG CCTGAACATC 
CACATGCGCA TCCATACCGG CGAAAAGCCG TTCCAGTGCA AGCTATGCGA CAAGTGCTTC 
ACACAGGCCT CCAGTCTGAG TGTTCACATG AAGATCCATG CGGGCGAGAA GCCCTACCCG 
TGTCCCATAT GCGGCAAGTC GTACAGCCAG CAGGCGTACC TCAACAAGCA CATCCAGGCG 
CACCAGATGG CTTCGGCCGC CTCCGCATCC ACGTCGCCTG GCCTGCTGGT CGCCAAGCAG 
CCGCACGAAA CACTCGTTTG CATTGTCTGC GGATCGTTGC ATGCGGATGC CACTGCACTG 
GCCAGCCATG TGCACAGCCA GCACGCAGCG CTGCTGGACA CGATGAAGCA GTCCGGAATG 
AACACGGCGC CGGGCGGAGC GATTCCAGAC GTCAAGTGCA GTGCAGAGGA GCAGCAAGCC 
TATGTGGAGC GGGTTCAGTG CGTCCTGCAG CAGATGAACC ATCAGCAGCA GCATCAACAG 
CACCAGCAAC AACCTCCGCA ACAACAACAG CAACATCCGC AACAACAACA GCAGCAGCAT 
CTGCAACAAC AGCCGCATCA GATGCAGCTA CCGCAGCAGC CCAAGTTGCC AGCCATGGAC 
TCCACCGGCG AGGACGAAGA AGAGCCCGAC GAAGCGGAGG AACCGCCGGA TGAGGAGGAG 
GAACAAGATG AACCAGAGGA GCCGGCGGAG GTGAAAACGG AAGTGCTGGC AGCAGAGGAC 
GCTCTCACCA ATCCGGGCTA TCCCATTCTC GGGCTGCAGG AGGAACAGAT CCTGCTAGAC 
TCTGACATGT ACTACGAGGA CTTCGGCGAC ATGGACGTGG GCTGTCAGGA GGAGGTGTTC 
GGCGACTTTG TCGTCAACGA GGAGGAGGTT TACACCGATG CGCTCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609448.1
CDS	436..2646
Translation	MAVQLKQSYHHLPLTITPILQQTHLLPQQAPNPPQQQPQQPQPPDLTFHCMCCAEFFVHPLALYQHMNTLHPHEPGNGQQEQESPGDESEDYSWIFEPVCELAEDGSDSSDGSASSGSDSSSSSDDDDDDDDDDSSSSSSSSSNSSSSSSSVPTTSNSNTQQSQESVQPLHGLVAGPGYNEFQLQMTDPRESTSIFMVQPTVSVTPLQQLLPPAPTVSPGLGLQSTPIKRRRGRRSNIGAPVMDPALNGNQKCFQCTHCEASFPNAGDLSKHVRSHITNKPFQCSICQKTFTHIGSLNTHIRIHSGEKPYKCELCPKAFTQSSSLMVHMRSHSVRKPHQCVQCDKGFINYSSLLLHQKTHIAPTETFICPECEREFKAEALLDEHMRMHTQELVYQCAICREAFRASSELVQHMKNHMGEKPFTCSLCDRSFTQSGSLNIHMRIHTGEKPFQCKLCDKCFTQASSLSVHMKIHAGEKPYPCPICGKSYSQQAYLNKHIQAHQMASAASASTSPGLLVAKQPHETLVCIVCGSLHADATALASHVHSQHAALLDTMKQSGMNTAPGGAIPDVKCSAEEQQAYVERVQCVLQQMNHQQQHQQHQQQPPQQQQQHPQQQQQQHLQQQPHQMQLPQQPKLPAMDSTGEDEEEPDEAEEPPDEEEEQDEPEEPAEVKTEVLAAEDALTNPGYPILGLQEEQILLDSDMYYEDFGDMDVGCQEEVFGDFVVNEEEVYTDALI

Target ORF information:

RefSeq Version	NM_135604.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG12299), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135604.3

ORF Insert Sequence:

ATGGCCGTGC AGCTGAAACA GAGCTACCAC CACCTGCCCC TGACGATCAC GCCAATCCTG 
CAGCAGACGC ACCTCCTGCC GCAACAGGCA CCCAATCCAC CCCAGCAGCA ACCGCAGCAG 
CCACAGCCGC CGGATCTCAC CTTCCACTGC ATGTGCTGTG CGGAGTTCTT CGTCCATCCG 
CTGGCGCTGT ACCAGCACAT GAACACCCTG CACCCCCATG AGCCGGGCAA TGGACAGCAA 
GAGCAGGAGA GCCCGGGCGA CGAGAGCGAG GACTACAGCT GGATATTCGA GCCGGTGTGC 
GAGCTGGCGG AGGATGGCAG CGACTCGTCG GATGGGAGCG CCTCCTCGGG CTCCGACAGC 
TCCTCGTCGT CGGACGACGA CGATGACGAT GACGACGACG ACAGCAGCAG CTCCAGTTCG 
AGTTCCAGCA ACTCGAGCTC ATCAAGCAGC TCGGTGCCGA CCACCAGCAA CTCCAATACC 
CAGCAGAGCC AGGAGTCAGT GCAGCCACTT CACGGCCTGG TTGCCGGTCC GGGCTACAAT 
GAGTTCCAGC TGCAGATGAC AGATCCGCGG GAGTCCACCT CCATATTCAT GGTGCAGCCG 
ACCGTAAGCG TCACGCCCTT GCAGCAGCTA CTACCACCGG CACCCACTGT TAGCCCGGGA 
TTGGGCCTGC AATCGACGCC CATCAAGCGG CGGCGCGGAA GGCGGAGCAA CATCGGTGCA 
CCCGTGATGG ATCCAGCGTT GAATGGCAAC CAGAAATGCT TCCAGTGCAC GCACTGCGAG 
GCGAGCTTTC CCAACGCCGG CGATCTGTCC AAGCACGTGC GCTCGCACAT CACCAACAAG 
CCGTTCCAGT GCTCCATCTG CCAGAAGACA TTCACGCACA TCGGCAGCCT GAACACGCAC 
ATCCGCATCC ACAGCGGCGA GAAGCCGTAC AAGTGCGAGC TCTGCCCGAA GGCCTTCACG 
CAGTCCAGCA GCCTGATGGT GCACATGCGC TCGCATTCGG TGCGCAAGCC GCATCAGTGT 
GTGCAGTGCG ACAAGGGATT CATCAACTAC AGCTCGCTGC TGCTGCACCA GAAGACGCAC 
ATCGCGCCCA CGGAGACGTT CATCTGTCCG GAGTGCGAGC GCGAATTCAA GGCGGAGGCG 
CTGCTCGACG AGCACATGCG CATGCATACG CAGGAGCTGG TGTACCAGTG CGCCATCTGC 
CGCGAGGCCT TCCGCGCCAG CTCGGAGCTG GTACAGCACA TGAAGAACCA CATGGGCGAG 
AAGCCATTCA CCTGCTCGCT GTGCGACCGC TCGTTCACGC AGTCGGGTAG CCTGAACATC 
CACATGCGCA TCCATACCGG CGAAAAGCCG TTCCAGTGCA AGCTATGCGA CAAGTGCTTC 
ACACAGGCCT CCAGTCTGAG TGTTCACATG AAGATCCATG CGGGCGAGAA GCCCTACCCG 
TGTCCCATAT GCGGCAAGTC GTACAGCCAG CAGGCGTACC TCAACAAGCA CATCCAGGCG 
CACCAGATGG CTTCGGCCGC CTCCGCATCC ACGTCGCCTG GCCTGCTGGT CGCCAAGCAG 
CCGCACGAAA CACTCGTTTG CATTGTCTGC GGATCGTTGC ATGCGGATGC CACTGCACTG 
GCCAGCCATG TGCACAGCCA GCACGCAGCG CTGCTGGACA CGATGAAGCA GTCCGGAATG 
AACACGGCGC CGGGCGGAGC GATTCCAGAC GTCAAGTGCA GTGCAGAGGA GCAGCAAGCC 
TATGTGGAGC GGGTTCAGTG CGTCCTGCAG CAGATGAACC ATCAGCAGCA GCATCAACAG 
CACCAGCAAC AACCTCCGCA ACAACAACAG CAACATCCGC AACAACAACA GCAGCAGCAT 
CTGCAACAAC AGCCGCATCA GATGCAGCTA CCGCAGCAGC CCAAGTTGCC AGCCATGGAC 
TCCACCGGCG AGGACGAAGA AGAGCCCGAC GAAGCGGAGG AACCGCCGGA TGAGGAGGAG 
GAACAAGATG AACCAGAGGA GCCGGCGGAG GTGAAAACGG AAGTGCTGGC AGCAGAGGAC 
GCTCTCACCA ATCCGGGCTA TCCCATTCTC GGGCTGCAGG AGGAACAGAT CCTGCTAGAC 
TCTGACATGT ACTACGAGGA CTTCGGCGAC ATGGACGTGG GCTGTCAGGA GGAGGTGTTC 
GGCGACTTTG TCGTCAACGA GGAGGAGGTT TACACCGATG CGCTCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG12299 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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