da cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	da
Entrez Gene ID	34413
Full Name	daughterless
Synonyms	5102,CG5102,DA,Da,Dmel\CG5102,SC_DA,bHLHb22,l(2)31Ea,l(2)k08611
General protein information	Preferred Names daughterless Names CG5102 gene product from transcript CG5102-RB CG5102-PA CG5102-PB CG5102-PC da-PA da-PB da-PC daughter-less lethal (2) k08611
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 31D11-31E1\|2-41.3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273411.1	NP_001260340.1	daughterless, isoform B
NM_001370036.1	NP_001356956.1	daughterless, isoform D
NM_057841.4	NP_477189.1	daughterless, isoform A
NM_001273412.1	NP_001260341.1	daughterless, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Daughterless and Tcf4 are expressed in postmitotic neurons in Drosophila melanogaster and mice, respectively, where they function to restrict neurite branching and synapse formation in part by restricting the expression of the cell adhesion molecule Neurexin.
Title: Type I bHLH Proteins Daughterless and Tcf4 Restrict Neurite Branching and Synapse Formation by Repressing Neurexin in Postmitotic Neurons.

Notch signaling activates the expression of extramacrochaetae (emc), which encodes a helix-loop-helix (HLH) transcription factor. Emc, in turn, then forms a biochemical complex with Da.
Title: Inhibition of Daughterless by Extramacrochaetae mediates Notch-induced cell proliferation.

Data show that bHLHb22 protein daughterless (Da) binding alters the turnover parameters of bHLHc28 protein scute (Sc).
Title: bHLH proteins involved in Drosophila neurogenesis are mutually regulated at the level of stability.

we show that during the development of epithelial cells in the imaginal discs, the G2/M transition, and hence cell proliferation, is controlled by Emc via Da. In eukaryotic cells, the main activator of this transition is the Cdc25 phosphatase, string
Title: The bHLH factors extramacrochaetae and daughterless control cell cycle in Drosophila imaginal discs through the transcriptional regulation of the Cdc25 phosphatase string.

We explain this paradox by demonstrating that Da induces the differentiation factor Prospero (Pros) whose asymmetric segregation is essential for differentiation in one of the two daughter cells.
Title: A regulatory transcriptional loop controls proliferation and differentiation in Drosophila neural stem cells.

The following da gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the da cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14342	NM_001273411.1 Latest version!	Drosophila melanogaster daughterless (da), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa14342	NM_057841.4 Latest version!	Drosophila melanogaster daughterless (da), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa14342	NM_001273412.1 Latest version!	Drosophila melanogaster daughterless (da), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa87747	NM_001370036.1 Latest version!	Drosophila melanogaster daughterless (da), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

da ( NM_001273411.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260340.1 Drosophila melanogaster daughterless (da), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14342
Clone ID Related Accession (Same CDS sequence)	NM_001273411.1 , NM_057841.4 , NM_001273412.1
Accession Version	NM_001273411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	daughterless, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGACCA GTGACGATGA GCCGATGCAC TTGTATGAGG TGTTCCAGAA CTGCTTCAAC 
AAGATAGCCA ACAAGCAACC GACGGGGACG GTTGGAGCGG ATCGCGGCGG TGGCGGTGGC 
TACCACTCGC CGTACGGCAG TCTGGGCGTG GAAAATGGCA TGTACCCCAG TGACTTCAAC 
TCGATGCACG ATACCGTCAA TGGCGGCAAC AACCGATATG CCAACGCCTC GACCGTCGAT 
CAGTATTTCG ATTCAGCTGC CGCCGGCAGC GGAGGTGCAT GGTGTCAGCC GCAGATGAGC 
AGCGCCAATA GCTACATGGG CCAATCGGCG TATCAGAATA GCGGCCCGCT CTCTGGCCAC 
TCGATAGACC AGCAGCAGCA GCAGGTTCAC CAGGCGGACG GACTCGGAAT GGGCGGAGGT 
GGTGGTGGCG GCGTCGGTGC CGATGGTATG CACTGCCCGG TGACCACGGG TCTGCCGCCG 
ATTAGCTCTT TTCGTCCCAC CTCCGGTGGA ATTGGAGGAC CTGGTGCCGG GCAGCAGGCA 
CCAGTGAACG TTAATGTGAA TCCCCCAGCC GTGTTCAATT CGCCGCAGGC CCACAACCAT 
AACCACACAG TACAAGCACA GCACAGTGCA CTGTCCACGG CTGGACCGCT TGGCCATCAC 
TCGCTCAACC ACACGCCGCA CGCACATTCC CACACACTCC CGCTCCCGCA CGCCCTGCCC 
CATGGCCATA CCCTTCCACA CCCCCACCAC AGTCAGCAGA ACAGTCCTGC CGTGCAGAGT 
TCGGATGCGT TCAGTGGTGC CGGGGCCAGT GTGAAGGTGG CCGGAGCTGG TAATTCCTCG 
GCAGCGGCAC TGCGGCAACA GATGTACATG CCGGCGGATC AAAGTATTAG TAGTTTCGGC 
TCGAACCCCT CCACGCCCGT CAACTCGCCT CCGCCGCTGA CCCAGTCGGT GGTTGGTGGC 
GGCGGCGAGC CGTCCGTGTC TGGCGGCTCT GGATGGGGTC ATTCGGTGCT AAATGGCGGA 
CCCAGCTCGA GCTACGCCAG CGAAATGGTT CCTGTGTCCA GCTTGCACAC CATGGCCTCG 
GTGTTCCAGG GCGTTCGCAT GGAGGAGCGA CTGGACGACG CTCTGAATGT GCTGCGGAAT 
CACTGCGAGC CCGAGATGCT GGCCGGTGTC AATCAGTCGC TGGCCTCGAT CGACAACATT 
GATGCGTTGA CCTCGTTTGT GCCCAACTCA CCGTCGCATC TGGGTAGCGG TGGGAACTCG 
GGCTCGGTCA GCAACACGTC CAATGCAGCA CTTGTGCACG AGGTTCTGGC CTTGGGCGCC 
GCTGCTGCTG CCGGCACATC CGGCCAATCG GTAGGCGGGG CAGGAAGCCT GGCCAGTTTG 
AAACTCGATC GCAGTGCGTC CACGTCCTTG CCTAAGCAAA CGAAAAAGCG GAAAGAGCAC 
ACAGCCATTA GCAATTCGGT TCCGGCGGGC GTGTCCACCA CCTCGAGTCT GACCAGCCTT 
GACATTTCGG ACACGAAGCC GACCAGCTCC ATTGAATCGT CGAATTCGGG ACTGCAGCAG 
CATTCGCAGG GAAAGGGAAC GAAAAGACCG AGACGCTACT GCTCCTCGGC GGACGAGGAT 
GATGACGCCG AGCCGGCAGT GAAGGCCATT CGCGAAAAGG AGCGGCGGCA GGCAAATAAT 
GCGCGCGAAC GTATCCGCAT TCGGGATATT AACGAGGCGC TCAAGGAGCT GGGCCGCATG 
TGCATGACGC ACCTGAAGTC CGACAAGCCG CAGACGAAGC TGGGCATCCT GAATATGGCC 
GTCGAGGTGA TCATGACGCT GGAGCAGCAG GTGCGCGAGC GCAACCTGAA TCCGAAGGCG 
GCGTGCCTGA AGCGGCGCGA GGAGGAGAAG GCCGAGGATG GTCCCAAGCT AAGTGCCCAG 
CATCACATGA TACCGCAGCC GCAGCAGGTG GGCGGCACGC CCGGCAGCAG CTATCATAGC 
CAGCCAGCGC AACTGGTGCC GCCATCATCG CAGACCATCA GCACCATGAC CATATCCCTA 
CCCGTCAACC AGGCCAACAA CGGCCTGCCA CCCCACTTGC AGCAGCAGCA GCAGCAACAA 
TCACAGCTCG GACACGCCCA GCTTCCGCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260340.1
CDS	437..2569
Translation	MATSDDEPMHLYEVFQNCFNKIANKQPTGTVGADRGGGGGYHSPYGSLGVENGMYPSDFNSMHDTVNGGNNRYANASTVDQYFDSAAAGSGGAWCQPQMSSANSYMGQSAYQNSGPLSGHSIDQQQQQVHQADGLGMGGGGGGGVGADGMHCPVTTGLPPISSFRPTSGGIGGPGAGQQAPVNVNVNPPAVFNSPQAHNHNHTVQAQHSALSTAGPLGHHSLNHTPHAHSHTLPLPHALPHGHTLPHPHHSQQNSPAVQSSDAFSGAGASVKVAGAGNSSAAALRQQMYMPADQSISSFGSNPSTPVNSPPPLTQSVVGGGGEPSVSGGSGWGHSVLNGGPSSSYASEMVPVSSLHTMASVFQGVRMEERLDDALNVLRNHCEPEMLAGVNQSLASIDNIDALTSFVPNSPSHLGSGGNSGSVSNTSNAALVHEVLALGAAAAAGTSGQSVGGAGSLASLKLDRSASTSLPKQTKKRKEHTAISNSVPAGVSTTSSLTSLDISDTKPTSSIESSNSGLQQHSQGKGTKRPRRYCSSADEDDDAEPAVKAIREKERRQANNARERIRIRDINEALKELGRMCMTHLKSDKPQTKLGILNMAVEVIMTLEQQVRERNLNPKAACLKRREEEKAEDGPKLSAQHHMIPQPQQVGGTPGSSYHSQPAQLVPPSSQTISTMTISLPVNQANNGLPPHLQQQQQQQSQLGHAQLPQ

Target ORF information:

RefSeq Version	NM_001273411.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster daughterless (da), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273411.1

ORF Insert Sequence:

ATGGCGACCA GTGACGATGA GCCGATGCAC TTGTATGAGG TGTTCCAGAA CTGCTTCAAC 
AAGATAGCCA ACAAGCAACC GACGGGGACG GTTGGAGCGG ATCGCGGCGG TGGCGGTGGC 
TACCACTCGC CGTACGGCAG TCTGGGCGTG GAAAATGGCA TGTACCCCAG TGACTTCAAC 
TCGATGCACG ATACCGTCAA TGGCGGCAAC AACCGATATG CCAACGCCTC GACCGTCGAT 
CAGTATTTCG ATTCAGCTGC CGCCGGCAGC GGAGGTGCAT GGTGTCAGCC GCAGATGAGC 
AGCGCCAATA GCTACATGGG CCAATCGGCG TATCAGAATA GCGGCCCGCT CTCTGGCCAC 
TCGATAGACC AGCAGCAGCA GCAGGTTCAC CAGGCGGACG GACTCGGAAT GGGCGGAGGT 
GGTGGTGGCG GCGTCGGTGC CGATGGTATG CACTGCCCGG TGACCACGGG TCTGCCGCCG 
ATTAGCTCTT TTCGTCCCAC CTCCGGTGGA ATTGGAGGAC CTGGTGCCGG GCAGCAGGCA 
CCAGTGAACG TTAATGTGAA TCCCCCAGCC GTGTTCAATT CGCCGCAGGC CCACAACCAT 
AACCACACAG TACAAGCACA GCACAGTGCA CTGTCCACGG CTGGACCGCT TGGCCATCAC 
TCGCTCAACC ACACGCCGCA CGCACATTCC CACACACTCC CGCTCCCGCA CGCCCTGCCC 
CATGGCCATA CCCTTCCACA CCCCCACCAC AGTCAGCAGA ACAGTCCTGC CGTGCAGAGT 
TCGGATGCGT TCAGTGGTGC CGGGGCCAGT GTGAAGGTGG CCGGAGCTGG TAATTCCTCG 
GCAGCGGCAC TGCGGCAACA GATGTACATG CCGGCGGATC AAAGTATTAG TAGTTTCGGC 
TCGAACCCCT CCACGCCCGT CAACTCGCCT CCGCCGCTGA CCCAGTCGGT GGTTGGTGGC 
GGCGGCGAGC CGTCCGTGTC TGGCGGCTCT GGATGGGGTC ATTCGGTGCT AAATGGCGGA 
CCCAGCTCGA GCTACGCCAG CGAAATGGTT CCTGTGTCCA GCTTGCACAC CATGGCCTCG 
GTGTTCCAGG GCGTTCGCAT GGAGGAGCGA CTGGACGACG CTCTGAATGT GCTGCGGAAT 
CACTGCGAGC CCGAGATGCT GGCCGGTGTC AATCAGTCGC TGGCCTCGAT CGACAACATT 
GATGCGTTGA CCTCGTTTGT GCCCAACTCA CCGTCGCATC TGGGTAGCGG TGGGAACTCG 
GGCTCGGTCA GCAACACGTC CAATGCAGCA CTTGTGCACG AGGTTCTGGC CTTGGGCGCC 
GCTGCTGCTG CCGGCACATC CGGCCAATCG GTAGGCGGGG CAGGAAGCCT GGCCAGTTTG 
AAACTCGATC GCAGTGCGTC CACGTCCTTG CCTAAGCAAA CGAAAAAGCG GAAAGAGCAC 
ACAGCCATTA GCAATTCGGT TCCGGCGGGC GTGTCCACCA CCTCGAGTCT GACCAGCCTT 
GACATTTCGG ACACGAAGCC GACCAGCTCC ATTGAATCGT CGAATTCGGG ACTGCAGCAG 
CATTCGCAGG GAAAGGGAAC GAAAAGACCG AGACGCTACT GCTCCTCGGC GGACGAGGAT 
GATGACGCCG AGCCGGCAGT GAAGGCCATT CGCGAAAAGG AGCGGCGGCA GGCAAATAAT 
GCGCGCGAAC GTATCCGCAT TCGGGATATT AACGAGGCGC TCAAGGAGCT GGGCCGCATG 
TGCATGACGC ACCTGAAGTC CGACAAGCCG CAGACGAAGC TGGGCATCCT GAATATGGCC 
GTCGAGGTGA TCATGACGCT GGAGCAGCAG GTGCGCGAGC GCAACCTGAA TCCGAAGGCG 
GCGTGCCTGA AGCGGCGCGA GGAGGAGAAG GCCGAGGATG GTCCCAAGCT AAGTGCCCAG 
CATCACATGA TACCGCAGCC GCAGCAGGTG GGCGGCACGC CCGGCAGCAG CTATCATAGC 
CAGCCAGCGC AACTGGTGCC GCCATCATCG CAGACCATCA GCACCATGAC CATATCCCTA 
CCCGTCAACC AGGCCAACAA CGGCCTGCCA CCCCACTTGC AGCAGCAGCA GCAGCAACAA 
TCACAGCTCG GACACGCCCA GCTTCCGCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

da ( NM_057841.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477189.1 Drosophila melanogaster daughterless (da), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14342
Clone ID Related Accession (Same CDS sequence)	NM_001273411.1 , NM_057841.4 , NM_001273412.1
Accession Version	NM_057841.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	daughterless, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057841.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGACCA GTGACGATGA GCCGATGCAC TTGTATGAGG TGTTCCAGAA CTGCTTCAAC 
AAGATAGCCA ACAAGCAACC GACGGGGACG GTTGGAGCGG ATCGCGGCGG TGGCGGTGGC 
TACCACTCGC CGTACGGCAG TCTGGGCGTG GAAAATGGCA TGTACCCCAG TGACTTCAAC 
TCGATGCACG ATACCGTCAA TGGCGGCAAC AACCGATATG CCAACGCCTC GACCGTCGAT 
CAGTATTTCG ATTCAGCTGC CGCCGGCAGC GGAGGTGCAT GGTGTCAGCC GCAGATGAGC 
AGCGCCAATA GCTACATGGG CCAATCGGCG TATCAGAATA GCGGCCCGCT CTCTGGCCAC 
TCGATAGACC AGCAGCAGCA GCAGGTTCAC CAGGCGGACG GACTCGGAAT GGGCGGAGGT 
GGTGGTGGCG GCGTCGGTGC CGATGGTATG CACTGCCCGG TGACCACGGG TCTGCCGCCG 
ATTAGCTCTT TTCGTCCCAC CTCCGGTGGA ATTGGAGGAC CTGGTGCCGG GCAGCAGGCA 
CCAGTGAACG TTAATGTGAA TCCCCCAGCC GTGTTCAATT CGCCGCAGGC CCACAACCAT 
AACCACACAG TACAAGCACA GCACAGTGCA CTGTCCACGG CTGGACCGCT TGGCCATCAC 
TCGCTCAACC ACACGCCGCA CGCACATTCC CACACACTCC CGCTCCCGCA CGCCCTGCCC 
CATGGCCATA CCCTTCCACA CCCCCACCAC AGTCAGCAGA ACAGTCCTGC CGTGCAGAGT 
TCGGATGCGT TCAGTGGTGC CGGGGCCAGT GTGAAGGTGG CCGGAGCTGG TAATTCCTCG 
GCAGCGGCAC TGCGGCAACA GATGTACATG CCGGCGGATC AAAGTATTAG TAGTTTCGGC 
TCGAACCCCT CCACGCCCGT CAACTCGCCT CCGCCGCTGA CCCAGTCGGT GGTTGGTGGC 
GGCGGCGAGC CGTCCGTGTC TGGCGGCTCT GGATGGGGTC ATTCGGTGCT AAATGGCGGA 
CCCAGCTCGA GCTACGCCAG CGAAATGGTT CCTGTGTCCA GCTTGCACAC CATGGCCTCG 
GTGTTCCAGG GCGTTCGCAT GGAGGAGCGA CTGGACGACG CTCTGAATGT GCTGCGGAAT 
CACTGCGAGC CCGAGATGCT GGCCGGTGTC AATCAGTCGC TGGCCTCGAT CGACAACATT 
GATGCGTTGA CCTCGTTTGT GCCCAACTCA CCGTCGCATC TGGGTAGCGG TGGGAACTCG 
GGCTCGGTCA GCAACACGTC CAATGCAGCA CTTGTGCACG AGGTTCTGGC CTTGGGCGCC 
GCTGCTGCTG CCGGCACATC CGGCCAATCG GTAGGCGGGG CAGGAAGCCT GGCCAGTTTG 
AAACTCGATC GCAGTGCGTC CACGTCCTTG CCTAAGCAAA CGAAAAAGCG GAAAGAGCAC 
ACAGCCATTA GCAATTCGGT TCCGGCGGGC GTGTCCACCA CCTCGAGTCT GACCAGCCTT 
GACATTTCGG ACACGAAGCC GACCAGCTCC ATTGAATCGT CGAATTCGGG ACTGCAGCAG 
CATTCGCAGG GAAAGGGAAC GAAAAGACCG AGACGCTACT GCTCCTCGGC GGACGAGGAT 
GATGACGCCG AGCCGGCAGT GAAGGCCATT CGCGAAAAGG AGCGGCGGCA GGCAAATAAT 
GCGCGCGAAC GTATCCGCAT TCGGGATATT AACGAGGCGC TCAAGGAGCT GGGCCGCATG 
TGCATGACGC ACCTGAAGTC CGACAAGCCG CAGACGAAGC TGGGCATCCT GAATATGGCC 
GTCGAGGTGA TCATGACGCT GGAGCAGCAG GTGCGCGAGC GCAACCTGAA TCCGAAGGCG 
GCGTGCCTGA AGCGGCGCGA GGAGGAGAAG GCCGAGGATG GTCCCAAGCT AAGTGCCCAG 
CATCACATGA TACCGCAGCC GCAGCAGGTG GGCGGCACGC CCGGCAGCAG CTATCATAGC 
CAGCCAGCGC AACTGGTGCC GCCATCATCG CAGACCATCA GCACCATGAC CATATCCCTA 
CCCGTCAACC AGGCCAACAA CGGCCTGCCA CCCCACTTGC AGCAGCAGCA GCAGCAACAA 
TCACAGCTCG GACACGCCCA GCTTCCGCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477189.1
CDS	437..2569
Translation	MATSDDEPMHLYEVFQNCFNKIANKQPTGTVGADRGGGGGYHSPYGSLGVENGMYPSDFNSMHDTVNGGNNRYANASTVDQYFDSAAAGSGGAWCQPQMSSANSYMGQSAYQNSGPLSGHSIDQQQQQVHQADGLGMGGGGGGGVGADGMHCPVTTGLPPISSFRPTSGGIGGPGAGQQAPVNVNVNPPAVFNSPQAHNHNHTVQAQHSALSTAGPLGHHSLNHTPHAHSHTLPLPHALPHGHTLPHPHHSQQNSPAVQSSDAFSGAGASVKVAGAGNSSAAALRQQMYMPADQSISSFGSNPSTPVNSPPPLTQSVVGGGGEPSVSGGSGWGHSVLNGGPSSSYASEMVPVSSLHTMASVFQGVRMEERLDDALNVLRNHCEPEMLAGVNQSLASIDNIDALTSFVPNSPSHLGSGGNSGSVSNTSNAALVHEVLALGAAAAAGTSGQSVGGAGSLASLKLDRSASTSLPKQTKKRKEHTAISNSVPAGVSTTSSLTSLDISDTKPTSSIESSNSGLQQHSQGKGTKRPRRYCSSADEDDDAEPAVKAIREKERRQANNARERIRIRDINEALKELGRMCMTHLKSDKPQTKLGILNMAVEVIMTLEQQVRERNLNPKAACLKRREEEKAEDGPKLSAQHHMIPQPQQVGGTPGSSYHSQPAQLVPPSSQTISTMTISLPVNQANNGLPPHLQQQQQQQSQLGHAQLPQ

Target ORF information:

RefSeq Version	NM_057841.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster daughterless (da), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057841.4

ORF Insert Sequence:

ATGGCGACCA GTGACGATGA GCCGATGCAC TTGTATGAGG TGTTCCAGAA CTGCTTCAAC 
AAGATAGCCA ACAAGCAACC GACGGGGACG GTTGGAGCGG ATCGCGGCGG TGGCGGTGGC 
TACCACTCGC CGTACGGCAG TCTGGGCGTG GAAAATGGCA TGTACCCCAG TGACTTCAAC 
TCGATGCACG ATACCGTCAA TGGCGGCAAC AACCGATATG CCAACGCCTC GACCGTCGAT 
CAGTATTTCG ATTCAGCTGC CGCCGGCAGC GGAGGTGCAT GGTGTCAGCC GCAGATGAGC 
AGCGCCAATA GCTACATGGG CCAATCGGCG TATCAGAATA GCGGCCCGCT CTCTGGCCAC 
TCGATAGACC AGCAGCAGCA GCAGGTTCAC CAGGCGGACG GACTCGGAAT GGGCGGAGGT 
GGTGGTGGCG GCGTCGGTGC CGATGGTATG CACTGCCCGG TGACCACGGG TCTGCCGCCG 
ATTAGCTCTT TTCGTCCCAC CTCCGGTGGA ATTGGAGGAC CTGGTGCCGG GCAGCAGGCA 
CCAGTGAACG TTAATGTGAA TCCCCCAGCC GTGTTCAATT CGCCGCAGGC CCACAACCAT 
AACCACACAG TACAAGCACA GCACAGTGCA CTGTCCACGG CTGGACCGCT TGGCCATCAC 
TCGCTCAACC ACACGCCGCA CGCACATTCC CACACACTCC CGCTCCCGCA CGCCCTGCCC 
CATGGCCATA CCCTTCCACA CCCCCACCAC AGTCAGCAGA ACAGTCCTGC CGTGCAGAGT 
TCGGATGCGT TCAGTGGTGC CGGGGCCAGT GTGAAGGTGG CCGGAGCTGG TAATTCCTCG 
GCAGCGGCAC TGCGGCAACA GATGTACATG CCGGCGGATC AAAGTATTAG TAGTTTCGGC 
TCGAACCCCT CCACGCCCGT CAACTCGCCT CCGCCGCTGA CCCAGTCGGT GGTTGGTGGC 
GGCGGCGAGC CGTCCGTGTC TGGCGGCTCT GGATGGGGTC ATTCGGTGCT AAATGGCGGA 
CCCAGCTCGA GCTACGCCAG CGAAATGGTT CCTGTGTCCA GCTTGCACAC CATGGCCTCG 
GTGTTCCAGG GCGTTCGCAT GGAGGAGCGA CTGGACGACG CTCTGAATGT GCTGCGGAAT 
CACTGCGAGC CCGAGATGCT GGCCGGTGTC AATCAGTCGC TGGCCTCGAT CGACAACATT 
GATGCGTTGA CCTCGTTTGT GCCCAACTCA CCGTCGCATC TGGGTAGCGG TGGGAACTCG 
GGCTCGGTCA GCAACACGTC CAATGCAGCA CTTGTGCACG AGGTTCTGGC CTTGGGCGCC 
GCTGCTGCTG CCGGCACATC CGGCCAATCG GTAGGCGGGG CAGGAAGCCT GGCCAGTTTG 
AAACTCGATC GCAGTGCGTC CACGTCCTTG CCTAAGCAAA CGAAAAAGCG GAAAGAGCAC 
ACAGCCATTA GCAATTCGGT TCCGGCGGGC GTGTCCACCA CCTCGAGTCT GACCAGCCTT 
GACATTTCGG ACACGAAGCC GACCAGCTCC ATTGAATCGT CGAATTCGGG ACTGCAGCAG 
CATTCGCAGG GAAAGGGAAC GAAAAGACCG AGACGCTACT GCTCCTCGGC GGACGAGGAT 
GATGACGCCG AGCCGGCAGT GAAGGCCATT CGCGAAAAGG AGCGGCGGCA GGCAAATAAT 
GCGCGCGAAC GTATCCGCAT TCGGGATATT AACGAGGCGC TCAAGGAGCT GGGCCGCATG 
TGCATGACGC ACCTGAAGTC CGACAAGCCG CAGACGAAGC TGGGCATCCT GAATATGGCC 
GTCGAGGTGA TCATGACGCT GGAGCAGCAG GTGCGCGAGC GCAACCTGAA TCCGAAGGCG 
GCGTGCCTGA AGCGGCGCGA GGAGGAGAAG GCCGAGGATG GTCCCAAGCT AAGTGCCCAG 
CATCACATGA TACCGCAGCC GCAGCAGGTG GGCGGCACGC CCGGCAGCAG CTATCATAGC 
CAGCCAGCGC AACTGGTGCC GCCATCATCG CAGACCATCA GCACCATGAC CATATCCCTA 
CCCGTCAACC AGGCCAACAA CGGCCTGCCA CCCCACTTGC AGCAGCAGCA GCAGCAACAA 
TCACAGCTCG GACACGCCCA GCTTCCGCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

da ( NM_001273412.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260341.1 Drosophila melanogaster daughterless (da), transcript variant C, mRNA.

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CloneID	OFa14342
Clone ID Related Accession (Same CDS sequence)	NM_001273411.1 , NM_057841.4 , NM_001273412.1
Accession Version	NM_001273412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	daughterless, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGACCA GTGACGATGA GCCGATGCAC TTGTATGAGG TGTTCCAGAA CTGCTTCAAC 
AAGATAGCCA ACAAGCAACC GACGGGGACG GTTGGAGCGG ATCGCGGCGG TGGCGGTGGC 
TACCACTCGC CGTACGGCAG TCTGGGCGTG GAAAATGGCA TGTACCCCAG TGACTTCAAC 
TCGATGCACG ATACCGTCAA TGGCGGCAAC AACCGATATG CCAACGCCTC GACCGTCGAT 
CAGTATTTCG ATTCAGCTGC CGCCGGCAGC GGAGGTGCAT GGTGTCAGCC GCAGATGAGC 
AGCGCCAATA GCTACATGGG CCAATCGGCG TATCAGAATA GCGGCCCGCT CTCTGGCCAC 
TCGATAGACC AGCAGCAGCA GCAGGTTCAC CAGGCGGACG GACTCGGAAT GGGCGGAGGT 
GGTGGTGGCG GCGTCGGTGC CGATGGTATG CACTGCCCGG TGACCACGGG TCTGCCGCCG 
ATTAGCTCTT TTCGTCCCAC CTCCGGTGGA ATTGGAGGAC CTGGTGCCGG GCAGCAGGCA 
CCAGTGAACG TTAATGTGAA TCCCCCAGCC GTGTTCAATT CGCCGCAGGC CCACAACCAT 
AACCACACAG TACAAGCACA GCACAGTGCA CTGTCCACGG CTGGACCGCT TGGCCATCAC 
TCGCTCAACC ACACGCCGCA CGCACATTCC CACACACTCC CGCTCCCGCA CGCCCTGCCC 
CATGGCCATA CCCTTCCACA CCCCCACCAC AGTCAGCAGA ACAGTCCTGC CGTGCAGAGT 
TCGGATGCGT TCAGTGGTGC CGGGGCCAGT GTGAAGGTGG CCGGAGCTGG TAATTCCTCG 
GCAGCGGCAC TGCGGCAACA GATGTACATG CCGGCGGATC AAAGTATTAG TAGTTTCGGC 
TCGAACCCCT CCACGCCCGT CAACTCGCCT CCGCCGCTGA CCCAGTCGGT GGTTGGTGGC 
GGCGGCGAGC CGTCCGTGTC TGGCGGCTCT GGATGGGGTC ATTCGGTGCT AAATGGCGGA 
CCCAGCTCGA GCTACGCCAG CGAAATGGTT CCTGTGTCCA GCTTGCACAC CATGGCCTCG 
GTGTTCCAGG GCGTTCGCAT GGAGGAGCGA CTGGACGACG CTCTGAATGT GCTGCGGAAT 
CACTGCGAGC CCGAGATGCT GGCCGGTGTC AATCAGTCGC TGGCCTCGAT CGACAACATT 
GATGCGTTGA CCTCGTTTGT GCCCAACTCA CCGTCGCATC TGGGTAGCGG TGGGAACTCG 
GGCTCGGTCA GCAACACGTC CAATGCAGCA CTTGTGCACG AGGTTCTGGC CTTGGGCGCC 
GCTGCTGCTG CCGGCACATC CGGCCAATCG GTAGGCGGGG CAGGAAGCCT GGCCAGTTTG 
AAACTCGATC GCAGTGCGTC CACGTCCTTG CCTAAGCAAA CGAAAAAGCG GAAAGAGCAC 
ACAGCCATTA GCAATTCGGT TCCGGCGGGC GTGTCCACCA CCTCGAGTCT GACCAGCCTT 
GACATTTCGG ACACGAAGCC GACCAGCTCC ATTGAATCGT CGAATTCGGG ACTGCAGCAG 
CATTCGCAGG GAAAGGGAAC GAAAAGACCG AGACGCTACT GCTCCTCGGC GGACGAGGAT 
GATGACGCCG AGCCGGCAGT GAAGGCCATT CGCGAAAAGG AGCGGCGGCA GGCAAATAAT 
GCGCGCGAAC GTATCCGCAT TCGGGATATT AACGAGGCGC TCAAGGAGCT GGGCCGCATG 
TGCATGACGC ACCTGAAGTC CGACAAGCCG CAGACGAAGC TGGGCATCCT GAATATGGCC 
GTCGAGGTGA TCATGACGCT GGAGCAGCAG GTGCGCGAGC GCAACCTGAA TCCGAAGGCG 
GCGTGCCTGA AGCGGCGCGA GGAGGAGAAG GCCGAGGATG GTCCCAAGCT AAGTGCCCAG 
CATCACATGA TACCGCAGCC GCAGCAGGTG GGCGGCACGC CCGGCAGCAG CTATCATAGC 
CAGCCAGCGC AACTGGTGCC GCCATCATCG CAGACCATCA GCACCATGAC CATATCCCTA 
CCCGTCAACC AGGCCAACAA CGGCCTGCCA CCCCACTTGC AGCAGCAGCA GCAGCAACAA 
TCACAGCTCG GACACGCCCA GCTTCCGCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260341.1
CDS	437..2569
Translation	MATSDDEPMHLYEVFQNCFNKIANKQPTGTVGADRGGGGGYHSPYGSLGVENGMYPSDFNSMHDTVNGGNNRYANASTVDQYFDSAAAGSGGAWCQPQMSSANSYMGQSAYQNSGPLSGHSIDQQQQQVHQADGLGMGGGGGGGVGADGMHCPVTTGLPPISSFRPTSGGIGGPGAGQQAPVNVNVNPPAVFNSPQAHNHNHTVQAQHSALSTAGPLGHHSLNHTPHAHSHTLPLPHALPHGHTLPHPHHSQQNSPAVQSSDAFSGAGASVKVAGAGNSSAAALRQQMYMPADQSISSFGSNPSTPVNSPPPLTQSVVGGGGEPSVSGGSGWGHSVLNGGPSSSYASEMVPVSSLHTMASVFQGVRMEERLDDALNVLRNHCEPEMLAGVNQSLASIDNIDALTSFVPNSPSHLGSGGNSGSVSNTSNAALVHEVLALGAAAAAGTSGQSVGGAGSLASLKLDRSASTSLPKQTKKRKEHTAISNSVPAGVSTTSSLTSLDISDTKPTSSIESSNSGLQQHSQGKGTKRPRRYCSSADEDDDAEPAVKAIREKERRQANNARERIRIRDINEALKELGRMCMTHLKSDKPQTKLGILNMAVEVIMTLEQQVRERNLNPKAACLKRREEEKAEDGPKLSAQHHMIPQPQQVGGTPGSSYHSQPAQLVPPSSQTISTMTISLPVNQANNGLPPHLQQQQQQQSQLGHAQLPQ

Target ORF information:

RefSeq Version	NM_001273412.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster daughterless (da), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273412.1

ORF Insert Sequence:

ATGGCGACCA GTGACGATGA GCCGATGCAC TTGTATGAGG TGTTCCAGAA CTGCTTCAAC 
AAGATAGCCA ACAAGCAACC GACGGGGACG GTTGGAGCGG ATCGCGGCGG TGGCGGTGGC 
TACCACTCGC CGTACGGCAG TCTGGGCGTG GAAAATGGCA TGTACCCCAG TGACTTCAAC 
TCGATGCACG ATACCGTCAA TGGCGGCAAC AACCGATATG CCAACGCCTC GACCGTCGAT 
CAGTATTTCG ATTCAGCTGC CGCCGGCAGC GGAGGTGCAT GGTGTCAGCC GCAGATGAGC 
AGCGCCAATA GCTACATGGG CCAATCGGCG TATCAGAATA GCGGCCCGCT CTCTGGCCAC 
TCGATAGACC AGCAGCAGCA GCAGGTTCAC CAGGCGGACG GACTCGGAAT GGGCGGAGGT 
GGTGGTGGCG GCGTCGGTGC CGATGGTATG CACTGCCCGG TGACCACGGG TCTGCCGCCG 
ATTAGCTCTT TTCGTCCCAC CTCCGGTGGA ATTGGAGGAC CTGGTGCCGG GCAGCAGGCA 
CCAGTGAACG TTAATGTGAA TCCCCCAGCC GTGTTCAATT CGCCGCAGGC CCACAACCAT 
AACCACACAG TACAAGCACA GCACAGTGCA CTGTCCACGG CTGGACCGCT TGGCCATCAC 
TCGCTCAACC ACACGCCGCA CGCACATTCC CACACACTCC CGCTCCCGCA CGCCCTGCCC 
CATGGCCATA CCCTTCCACA CCCCCACCAC AGTCAGCAGA ACAGTCCTGC CGTGCAGAGT 
TCGGATGCGT TCAGTGGTGC CGGGGCCAGT GTGAAGGTGG CCGGAGCTGG TAATTCCTCG 
GCAGCGGCAC TGCGGCAACA GATGTACATG CCGGCGGATC AAAGTATTAG TAGTTTCGGC 
TCGAACCCCT CCACGCCCGT CAACTCGCCT CCGCCGCTGA CCCAGTCGGT GGTTGGTGGC 
GGCGGCGAGC CGTCCGTGTC TGGCGGCTCT GGATGGGGTC ATTCGGTGCT AAATGGCGGA 
CCCAGCTCGA GCTACGCCAG CGAAATGGTT CCTGTGTCCA GCTTGCACAC CATGGCCTCG 
GTGTTCCAGG GCGTTCGCAT GGAGGAGCGA CTGGACGACG CTCTGAATGT GCTGCGGAAT 
CACTGCGAGC CCGAGATGCT GGCCGGTGTC AATCAGTCGC TGGCCTCGAT CGACAACATT 
GATGCGTTGA CCTCGTTTGT GCCCAACTCA CCGTCGCATC TGGGTAGCGG TGGGAACTCG 
GGCTCGGTCA GCAACACGTC CAATGCAGCA CTTGTGCACG AGGTTCTGGC CTTGGGCGCC 
GCTGCTGCTG CCGGCACATC CGGCCAATCG GTAGGCGGGG CAGGAAGCCT GGCCAGTTTG 
AAACTCGATC GCAGTGCGTC CACGTCCTTG CCTAAGCAAA CGAAAAAGCG GAAAGAGCAC 
ACAGCCATTA GCAATTCGGT TCCGGCGGGC GTGTCCACCA CCTCGAGTCT GACCAGCCTT 
GACATTTCGG ACACGAAGCC GACCAGCTCC ATTGAATCGT CGAATTCGGG ACTGCAGCAG 
CATTCGCAGG GAAAGGGAAC GAAAAGACCG AGACGCTACT GCTCCTCGGC GGACGAGGAT 
GATGACGCCG AGCCGGCAGT GAAGGCCATT CGCGAAAAGG AGCGGCGGCA GGCAAATAAT 
GCGCGCGAAC GTATCCGCAT TCGGGATATT AACGAGGCGC TCAAGGAGCT GGGCCGCATG 
TGCATGACGC ACCTGAAGTC CGACAAGCCG CAGACGAAGC TGGGCATCCT GAATATGGCC 
GTCGAGGTGA TCATGACGCT GGAGCAGCAG GTGCGCGAGC GCAACCTGAA TCCGAAGGCG 
GCGTGCCTGA AGCGGCGCGA GGAGGAGAAG GCCGAGGATG GTCCCAAGCT AAGTGCCCAG 
CATCACATGA TACCGCAGCC GCAGCAGGTG GGCGGCACGC CCGGCAGCAG CTATCATAGC 
CAGCCAGCGC AACTGGTGCC GCCATCATCG CAGACCATCA GCACCATGAC CATATCCCTA 
CCCGTCAACC AGGCCAACAA CGGCCTGCCA CCCCACTTGC AGCAGCAGCA GCAGCAACAA 
TCACAGCTCG GACACGCCCA GCTTCCGCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

da ( NM_001370036.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001356956.1 Drosophila melanogaster daughterless (da), transcript variant D, mRNA.

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CloneID	OFa87747
Clone ID Related Accession (Same CDS sequence)	NM_001370036.1
Accession Version	NM_001370036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	daughterless, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGACCA GTGACGATGA GCCGATGCAC TTGTATGAGG TGTTCCAGAA CTGCTTCAAC 
AAGATAGCCA ACAAGCAACC GACGGGGACG GTTGGAGCGG ATCGCGGCGG TGGCGGTGGC 
TACCACTCGC CGTACGGCAG TCTGGGCGTG GAAAATGGCA TGTACCCCAG TGACTTCAAC 
TCGATGCACG ATACCGTCAA TGGCGGCAAC AACCGATATG CCAACGCCTC GACCGTCGAT 
CAGTATTTCG ATTCAGCTGC CGCCGGCAGC GGAGGTGCAT GGTGTCAGCC GCAGATGAGC 
AGCGCCAATA GCTACATGGG CCAATCGGCG TATCAGAATA GCGGCCCGCT CTCTGGCCAC 
TCGATAGACC AGCAGCAGCA GCAGGTTCAC CAGGCGGACG GACTCGGAAT GGGCGGAGGT 
GGTGGTGGCG GCGTCGGTGC CGATGGTATG CACTGCCCGG TGACCACGGG TCTGCCGCCG 
ATTAGCTCTT TTCGTCCCAC CTCCGGTGGA ATTGGAGGAC CTGGTGCCGG GCAGCAGGCA 
CCAGTGAACG TTAATGTGAA TCCCCCAGCC GTGTTCAATT CGCCGCAGGC CCACAACCAT 
AACCACACAG TACAAGCACA GCACAGTGCA CTGTCCACGG CTGGACCGCT TGGCCATCAC 
TCGCTCAACC ACACGCCGCA CGCACATTCC CACACACTCC CGCTCCCGCA CGCCCTGCCC 
CATGGCCATA CCCTTCCACA CCCCCACCAC AGTCAGCAGA ACAGTCCTGC CGTGCAGAGT 
TCGGATGCGT TCAGTGGTGC CGGGGCCAGT GTGAAGGTGG CCGGAGCTGG TAATTCCTCG 
GCAGCGGCAC TGCGGCAACA GATGTACATG CCGGCGGATC AAAGTATTAG TAGTTTCGGC 
TCGAACCCCT CCACGCCCGT CAACTCGCCT CCGCCGCTGA CCCAGTCGGT GGTTGGTGGC 
GGCGGCGAGC CGTCCGTGTC TGGCGGCTCT GGATGGGGTC ATTCGGTGCT AAATGGCGGA 
CCCAGCTCGA GCTACGCCAG CGAAATGGTT CCTGTGTCCA GCTTGCACAC CATGGCCTCG 
GTGTTCCAGG GCGTTCGCAT GGAGGAGCGA CTGGACGACG CTCTGAATGT GCTGCGGAAT 
CACTGCGAGC CCGAGATGCT GGCCGGTGTC AATCAGTCGC TGGCCTCGAT CGACAACATT 
GATGCGTTGA CCTCGTTTGT GCCCAACTCA CCGTCGCATC TGGGTAGCGG TGGGAACTCG 
GGCTCGGTCA GCAACACGTC CAATGCAGCA CTTGTGCACG AGGTTCTGGC CTTGGGCGCC 
GCTGCTGCTG CCGGCACATC CGGCCAATCG GTAGGCGGGG CAGGAAGCCT GGCCAGTTTG 
AAACTCGATC GCAGTGCGTC CACGTCCTTG CCTAAGCAAA CGAAAAAGCG GAAAGAGCAC 
ACAGCCATTA GCAATTCGGT TCCGGCGGGC GTGTCCACCA CCTCGAGTCT GACCAGCCTT 
GACATTTCGG ACACGAAGCC GACCAGCTCC ATTGAATCGT CGAATTCGGG ACTGCAGCAG 
CATTCGCAGG GAAAGGGAAC GAAAAGACCG AGACGCTACT GCTCCTCGGC GGACGAGGAT 
GATGACGCCG AGCCGGCAGT GAAGGCCATT CGCGAAAAGG AGCGGCGGCA GGCAAATAAT 
GCGCGCGAAC GTATCCGCAT TCGGGATATT AACGAGGCGC TCAAGGAGCT GGGCCGCATG 
TGCATGACGC ACCTGAAGTC CGACAAGCCG CAGACGAAGC TGGGCATCCT GAATATGGCC 
GTCGAGGTGA TCATGACGCT GGAGCAGCAG GTGCGCGAGC GCAACCTGAA TCCGAAGGCG 
GCGTGCCTGA AGCGGCGCGA GGAGGAGAAG GCCGAGGATG GTCCCAAGCT AAGTGCCCAG 
CATCACATGA TACCGCAGCC GCAGCAGGTG GGCGGCACGC CCGGCAGCAG CTATCATAGC 
CAGCCAGCGC AACTGGTGCC GCCATCATCG CAGACCATCA GCACCATGAC CATATCCCTA 
CCCGTCAACC AGGCCAACAA CGGCCTGCCA CCCCACTTGC AGCAGCAGCA GCAGCAACAA 
TCACAGCTCG GACACGCCCA GCTTCCGCAA TAGCATGCAC CACCCAGGAA CCCCTTCTGG 
AAATCGTCGT CGTCCCTCTC TTCCATAAAA ACCGAATGTA TTGAAGGCTC CGCGGCCAAG 
TACTCAGATA ACTTGGAACT GGAGAATCAG GTTTTAAGCA ATATGTTCTG GCGTTGTCGT 
CCTCCCAAAT TGCAACCACA ACCCCTACAA AATGTACAAC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356956.1
CDS	437..2764
Translation	MATSDDEPMHLYEVFQNCFNKIANKQPTGTVGADRGGGGGYHSPYGSLGVENGMYPSDFNSMHDTVNGGNNRYANASTVDQYFDSAAAGSGGAWCQPQMSSANSYMGQSAYQNSGPLSGHSIDQQQQQVHQADGLGMGGGGGGGVGADGMHCPVTTGLPPISSFRPTSGGIGGPGAGQQAPVNVNVNPPAVFNSPQAHNHNHTVQAQHSALSTAGPLGHHSLNHTPHAHSHTLPLPHALPHGHTLPHPHHSQQNSPAVQSSDAFSGAGASVKVAGAGNSSAAALRQQMYMPADQSISSFGSNPSTPVNSPPPLTQSVVGGGGEPSVSGGSGWGHSVLNGGPSSSYASEMVPVSSLHTMASVFQGVRMEERLDDALNVLRNHCEPEMLAGVNQSLASIDNIDALTSFVPNSPSHLGSGGNSGSVSNTSNAALVHEVLALGAAAAAGTSGQSVGGAGSLASLKLDRSASTSLPKQTKKRKEHTAISNSVPAGVSTTSSLTSLDISDTKPTSSIESSNSGLQQHSQGKGTKRPRRYCSSADEDDDAEPAVKAIREKERRQANNARERIRIRDINEALKELGRMCMTHLKSDKPQTKLGILNMAVEVIMTLEQQVRERNLNPKAACLKRREEEKAEDGPKLSAQHHMIPQPQQVGGTPGSSYHSQPAQLVPPSSQTISTMTISLPVNQANNGLPPHLQQQQQQQSQLGHAQLPQ*HAPPRNPFWKSSSSLSSIKTECIEGSAAKYSDNLELENQVLSNMFWRCRPPKLQPQPLQNVQHF

Target ORF information:

RefSeq Version	NM_001370036.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster daughterless (da), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370036.1

ORF Insert Sequence:

ATGGCGACCA GTGACGATGA GCCGATGCAC TTGTATGAGG TGTTCCAGAA CTGCTTCAAC 
AAGATAGCCA ACAAGCAACC GACGGGGACG GTTGGAGCGG ATCGCGGCGG TGGCGGTGGC 
TACCACTCGC CGTACGGCAG TCTGGGCGTG GAAAATGGCA TGTACCCCAG TGACTTCAAC 
TCGATGCACG ATACCGTCAA TGGCGGCAAC AACCGATATG CCAACGCCTC GACCGTCGAT 
CAGTATTTCG ATTCAGCTGC CGCCGGCAGC GGAGGTGCAT GGTGTCAGCC GCAGATGAGC 
AGCGCCAATA GCTACATGGG CCAATCGGCG TATCAGAATA GCGGCCCGCT CTCTGGCCAC 
TCGATAGACC AGCAGCAGCA GCAGGTTCAC CAGGCGGACG GACTCGGAAT GGGCGGAGGT 
GGTGGTGGCG GCGTCGGTGC CGATGGTATG CACTGCCCGG TGACCACGGG TCTGCCGCCG 
ATTAGCTCTT TTCGTCCCAC CTCCGGTGGA ATTGGAGGAC CTGGTGCCGG GCAGCAGGCA 
CCAGTGAACG TTAATGTGAA TCCCCCAGCC GTGTTCAATT CGCCGCAGGC CCACAACCAT 
AACCACACAG TACAAGCACA GCACAGTGCA CTGTCCACGG CTGGACCGCT TGGCCATCAC 
TCGCTCAACC ACACGCCGCA CGCACATTCC CACACACTCC CGCTCCCGCA CGCCCTGCCC 
CATGGCCATA CCCTTCCACA CCCCCACCAC AGTCAGCAGA ACAGTCCTGC CGTGCAGAGT 
TCGGATGCGT TCAGTGGTGC CGGGGCCAGT GTGAAGGTGG CCGGAGCTGG TAATTCCTCG 
GCAGCGGCAC TGCGGCAACA GATGTACATG CCGGCGGATC AAAGTATTAG TAGTTTCGGC 
TCGAACCCCT CCACGCCCGT CAACTCGCCT CCGCCGCTGA CCCAGTCGGT GGTTGGTGGC 
GGCGGCGAGC CGTCCGTGTC TGGCGGCTCT GGATGGGGTC ATTCGGTGCT AAATGGCGGA 
CCCAGCTCGA GCTACGCCAG CGAAATGGTT CCTGTGTCCA GCTTGCACAC CATGGCCTCG 
GTGTTCCAGG GCGTTCGCAT GGAGGAGCGA CTGGACGACG CTCTGAATGT GCTGCGGAAT 
CACTGCGAGC CCGAGATGCT GGCCGGTGTC AATCAGTCGC TGGCCTCGAT CGACAACATT 
GATGCGTTGA CCTCGTTTGT GCCCAACTCA CCGTCGCATC TGGGTAGCGG TGGGAACTCG 
GGCTCGGTCA GCAACACGTC CAATGCAGCA CTTGTGCACG AGGTTCTGGC CTTGGGCGCC 
GCTGCTGCTG CCGGCACATC CGGCCAATCG GTAGGCGGGG CAGGAAGCCT GGCCAGTTTG 
AAACTCGATC GCAGTGCGTC CACGTCCTTG CCTAAGCAAA CGAAAAAGCG GAAAGAGCAC 
ACAGCCATTA GCAATTCGGT TCCGGCGGGC GTGTCCACCA CCTCGAGTCT GACCAGCCTT 
GACATTTCGG ACACGAAGCC GACCAGCTCC ATTGAATCGT CGAATTCGGG ACTGCAGCAG 
CATTCGCAGG GAAAGGGAAC GAAAAGACCG AGACGCTACT GCTCCTCGGC GGACGAGGAT 
GATGACGCCG AGCCGGCAGT GAAGGCCATT CGCGAAAAGG AGCGGCGGCA GGCAAATAAT 
GCGCGCGAAC GTATCCGCAT TCGGGATATT AACGAGGCGC TCAAGGAGCT GGGCCGCATG 
TGCATGACGC ACCTGAAGTC CGACAAGCCG CAGACGAAGC TGGGCATCCT GAATATGGCC 
GTCGAGGTGA TCATGACGCT GGAGCAGCAG GTGCGCGAGC GCAACCTGAA TCCGAAGGCG 
GCGTGCCTGA AGCGGCGCGA GGAGGAGAAG GCCGAGGATG GTCCCAAGCT AAGTGCCCAG 
CATCACATGA TACCGCAGCC GCAGCAGGTG GGCGGCACGC CCGGCAGCAG CTATCATAGC 
CAGCCAGCGC AACTGGTGCC GCCATCATCG CAGACCATCA GCACCATGAC CATATCCCTA 
CCCGTCAACC AGGCCAACAA CGGCCTGCCA CCCCACTTGC AGCAGCAGCA GCAGCAACAA 
TCACAGCTCG GACACGCCCA GCTTCCGCAA TAGCATGCAC CACCCAGGAA CCCCTTCTGG 
AAATCGTCGT CGTCCCTCTC TTCCATAAAA ACCGAATGTA TTGAAGGCTC CGCGGCCAAG 
TACTCAGATA ACTTGGAACT GGAGAATCAG GTTTTAAGCA ATATGTTCTG GCGTTGTCGT 
CCTCCCAAAT TGCAACCACA ACCCCTACAA AATGTACAAC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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da cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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