Npc1a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npc1a
Entrez Gene ID	34358
Full Name	Niemann-Pick type C-1a
Synonyms	CG5722,DmNPC,Dmel\CG5722,NPC1,NPC1a,Npc1,Npc1_Dm,dNPC1,dNPC1a,dmNPC1,dncp1a,dnpc1a,npc1,npc1a
General protein information	Preferred Names Niemann-Pick type C-1a Names CG5722 gene product from transcript CG5722-RD CG5722-PA CG5722-PB CG5722-PC CG5722-PD CG5722-PE CG5722-PF CG5722-PG Niemann-Pick type C-1 Npc1a-PA Npc1a-PB Npc1a-PC Npc1a-PD Npc1a-PE Npc1a-PF Npc1a-PG
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 31B1-31B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001201841.2	NP_001188770.1	Niemann-Pick type C-1a, isoform C
NM_135513.5	NP_609357.2	Niemann-Pick type C-1a, isoform A
NM_001298867.1	NP_001285796.1	Niemann-Pick type C-1a, isoform F
NM_001201840.2	NP_001188769.1	Niemann-Pick type C-1a, isoform B
NM_001201842.2	NP_001188771.1	Niemann-Pick type C-1a, isoform D
NM_001298866.1	NP_001285795.1	Niemann-Pick type C-1a, isoform E
NM_001298868.1	NP_001285797.1	Niemann-Pick type C-1a, isoform G

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NPC1	NP_001003107.1
Bos taurus (cattle)	NPC1	NP_777183.1
Kluyveromyces lactis	KLLA0F27137g	XP_456287.1
Magnaporthe oryzae (rice blast fungus)	MGG_00819	XP_003718164.1
Mus musculus (house mouse)	Npc1	NP_032746.2
Drosophila melanogaster (fruit fly)	Npc1a	NP_001188769.1
Homo sapiens (human)	NPC1	NP_000262.2
Pan troglodytes (chimpanzee)	NPC1	XP_001155285.1
	NPC1	XP_002800934.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008137	XP_317322.4
Rattus norvegicus (Norway rat)	Npc1	NP_705888.2
Danio rerio (zebrafish)	npc1	NP_001230804.1
Saccharomyces cerevisiae (baker's yeast)	NCR1	NP_015319.1
Eremothecium gossypii	AGOS_AGL008W	NP_986658.1
Neurospora crassa	NCU04090	XP_956086.1
Arabidopsis thaliana (thale cress)	AT1G42470	NP_174975.2
Gallus gallus (chicken)	NPC1	XP_419162.3
Arabidopsis thaliana (thale cress)	AT4G38350	NP_001190953.1
Xenopus tropicalis (tropical clawed frog)	npc1	XP_004915269.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a sterol-dependent, ecdysone-independent mechanism of NPC1 function in Drosophila spermatogenesis.
Title: The cholesterol trafficking protein NPC1 is required for Drosophila spermatogenesis.

These data demonstrate a requirement for dNPC1a in the maintenance of neuronal function and viability and show that loss of dNPC1a in neurons mimics the human neurodegenerative condition.
Title: Neuronal loss of Drosophila NPC1a causes cholesterol aggregation and age-progressive neurodegeneration.

The Niemann-Pick C proteins, in addition to their significant structural and sequence homology, these proteins may orchestrate their functions in a previously unappreciated fashion
Title: Niemann-Pick C proteins in sterol transport and absorption: flies in the ointment.

Mutations of a Drosophila NPC1 gene confer sterol and ecdysone metabolic defects
Title: Mutations of a Drosophila NPC1 gene confer sterol and ecdysone metabolic defects.

dnpc1a function is crucial for normal steroid hormone metabolism
Title: A Drosophila model of the Niemann-Pick type C lysosome storage disease: dnpc1a is required for molting and sterol homeostasis.

The following Npc1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npc1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14284	NM_001201841.2 Latest version!	Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa14284	NM_001201840.2 Latest version!	Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa14284	NM_135513.5 Latest version!	Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa14284	NM_001201842.2 Latest version!	Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa21622	NM_001298867.1 Latest version!	Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa21622	NM_001298866.1 Latest version!	Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa21622	NM_001298868.1 Latest version!	Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npc1a ( NM_001201841.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188770.1 Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa14284
Clone ID Related Accession (Same CDS sequence)	NM_001201841.2 , NM_135513.5 , NM_001201840.2 , NM_001201842.2
Accession Version	NM_001201841.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Niemann-Pick type C-1a, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001201841.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCCAA GATCCCCGCT CAGGATATCT CCATTTGGAG TACACATCCT CATCGCTGCT 
GTGCTCTTTA CGCTAATTCA ATCATCGAAA CAGGACTGCG TTTGGTATGG CGTGTGCAAC 
ACCAATGACT TTTCGCACAG CCAAAACTGT CCGTACAATG GAACAGCGAA GGAAATGGCA 
ACTGACGGTC TGGAGCTGCT GAAGAAACGC TGTGGATTTC TGCTGGAGAA TAGCGAAAAC 
AAATTTTGCT GCGATAAGAA TCAGGTGGAG CTCCTTAATA AGAATGTGGA GCTGGCCGGA 
AATATCTTGG ATCGATGTCC ATCCTGCATG GAGAACTTGG TGCGGCACAT CTGCCAATTC 
ACCTGTTCAC CAAAGCAGGC TGAGTTCATG CATGTGGTGG CCACGCAGAA AAATAAGAAA 
GGTGATGAGT ACATAAGTTC TGTTGACTTG CATATATCAA CGGAATACAT CAACAAGACT 
TATAAATCGT GCTCTCAGGT TTCTGTACCG CAAACGGGTC AATTAGCTTT TGATTTAATG 
TGCGGCGCTT ACTCAGCATC TCGCTGCAAT CCTACAAAAT GGTTCAACTT TATGGGCGAT 
GCCACCAATC CATATGTTCC TTTTCAAATT ACCTATATTC AACATGAGCC CAAATCAAAT 
AGCAATAACT TTACGCCCCT CAATGTAACG ACCGTTCCTT GCAACCAAGC AGTTAGCAGC 
AAACTACCTG CGTGCTCCTG CTCGGACTGC GACCTATCCT GTCCCCAGGG ACCGCCGGAA 
CCGCCCCGTC CAGAGCCCTT CAAAATTGTT GGACTCGACG CCTATTTCGT CATCATGGCA 
GCTGTCTTCT TGGTGGGAGT CTTAGTATTC TTAATGGGCT CGTTTCTGTT TACACAAGGT 
TCGTCCATGG ATGACAACTT TCAAGTGGAT GGCAATGATG TTTCCGATGA AATGCCCTAC 
AGCGAGAATG ATTCATACTT TGAAAAACTT GGCGCCCACA CAGAAACTTT CCTGGAAACA 
TTCTTCACCA AATGGGGAAC GTACTTTGCT AGCAATCCTG GCTTGACTTT AATTGCCGGA 
GCATCCCTGG TTGTTATACT GGGGTATGGC ATCAACTTTA TAGAAATCAC AACGGATCCG 
GTTAAACTTT GGGCTTCGCC GAACTCAAAA TCCAGACTGG AGAGAGAGTT TTTCGATACG 
AAGTTTTCGC CATTCTATAG GCTGGAACAG ATAATCATCA AAGCTGTGAA CTTGCCACAG 
ATTGTGCACA ACACCTCGAA CGGCCCATAC ACCTTCGGTC CCGTCTTCGA CAGAGAATTT 
CTAACAAAAG TGTTGGATCT GCAGGAGGGC ATCAAGGAAA TTAACGCAAA TGGCACACAG 
CTGAAGGATA TATGCTACGC GCCTTTGTCG GATGATGGCA GTGAAATCGA TGTGTCCCAG 
TGCGTTGTCC AATCGATTTG GGGATACTTT GGCGATGATC GAGAAAGATT GGATGACCAC 
GACGAGGACA ATGGATTCAA TGTTACCTAT TTGGATGCCT TATACGATTG CATTAGCAAT 
CCCTATTTAT GCCTAGCTCC TTATGGCGGT CCCGTAGATC CAGCCATAGC ACTTGGAGGA 
TTCCTGCCAC CTGGAGACCA ACTGACTGGC AGCACGAAAT TTGAGTTGGC CAATGCGATC 
ATTCTGACAT TCCTGGTCAA GAATCATCAT AACAAAACCG ATTTGGAAAA TGCCTTAACG 
TGGGAGAAGA AATTCGTGGA GTTTATGACC AACTATACGA AGAACAATAT GTCGCAGTAC 
ATGGACATAG CCTTCACATC GGAGAGATCG ATAGAAGACG AGCTCAACAG GGAGTCCCAG 
TCGGATGTGC TGACTATTTT GGTGTCCTAC CTAATTATGT TCATGTACAT AGCGATTTCG 
CTTGGCCATG TCAAGGAGTT CAAGCGAGTG TTTATCGACA GTAAGATAAC GCTTGGCATA 
GGCGGTGTCA TCATTGTTTT GGCCTCGGTA GTGTCGTCCG TTGGCGTTTT CGGTTACATC 
GGTTTGCCGG CCACCCTGAT CATTGTCGAG GTCATACCGT TTTTAGTATT AGCTGTCGGA 
GTGGATAACA TTTTTATTCT GGTCCAAACT CATCAGCGCG ATCAACGCAA ACCCAATGAA 
ACGCTTGAGC AGCAAGTTGG TCGAATACTC GGCAAGGTTG GACCCAGTAT GCTGCTCACC 
TCGTTGAGTG AAAGCTTTTG CTTCTTTCTC GGTGGCCTTT CTGATATGCC AGCCGTAAGG 
GCTTTTGCCC TATACGCCGG CGTAGCCCTC ATCATTGATT TTCTATTGCA AATAACCTGT 
TTCGTTAGCC TATTTACGTT GGATACGAAG CGCAGGGAGG AGAATCGTAT GGACATTTGT 
TGTTTCATCA AGGGCAAGAA GCCGGATAGT ATAACTAGTA ATGAGGGACT CTTGTACAAA 
TTTTTCAGTA GTGTTTACGT CCCATTTCTG ATGAAGAAAA TTGTGCGCGC CAGTGTGATG 
GTCATCTTTT TTGCCTGGCT GTGCTTCAGC ATTGCCATTG CGCCACGAAT CGACATTGGG 
CTGGATCAAG AGTTGGCTAT GCCGCAGGAT AGTTTCGTCT TGCACTATTT CCAATCGTTG 
AACGAGAATC TCAACATTGG TCCACCAGTG TACTTTGTGC TGAAGGGCGA TCTAGCCTAT 
ACGAATAGTT CGGATCAGAA TTTGGTGTGT GCAGGTCAAT ATTGCAACGA TGACAGCGTA 
CTCACGCAGA TCTACTTAGC CAGTAGGCAC TCTAACCAAA CGTATATCGC GCGTCCAGCA 
TCCAGTTGGA TCGATGACTA CTTCGATTGG GCGGCAGCAG CATCATCCTG TTGCAAATAT 
AGAAAGGATA GCGGCGACTT TTGTCCGCAT CAGGATACTT CCTGTTTGAG GTGTAATATC 
ACGAAGAATT CACTTCTTAG GCCGGAGGAA AAGGAGTTTG TTAAGTATTT GCCATTTTTC 
CTGAAGGACA ATCCCGATGA CACGTGCGCC AAGGCCGGTC ATGCCGCCTA TGGCGGAGCT 
GTTCGGTATT CCAACAGCCA TGAAAGACTG AACATCGAGG CGTCGTATTT TATGGCATAC 
CACACCATAC TTAAGTCCTC GGCCGACTAC TTTCTGGCCC TAGAGTCGGC TAGAAAGATA 
TCCGCGAATA TTACGCAAAT GTTGCAGGGC AGACTAATGT CAAATGGAGT TCCTATGGCA 
TCGGCCCTTA CGGTCGAAGT GTTTCCATAT TCAGTATTCT ATGTGTTTTA CGAACAATAT 
CTGACCATGT GGTCGGATAC GCTACAGAGC ATGGGTATTT CAGTTCTTTC CATATTCGTT 
GTTACATTTG TTTTGATGGG CTTCGATGTT CATTCCGCAT TAGTTGTTGT CATAACGATA 
ACTATGATTG TTGTTAATTT AGGTGGCCTT ATGTATTATT GGAACATTTC GTTGAATGCT 
GTTTCGTTGG TGAATCTCGT CATGGCCGTC GGCATATCCG TGGAATTTTG TTCGCACTTG 
GTGCACAGCT TCGCCACATC GAAATCAGTT AGTCAAATTG ATCGAGCTGC AGATAGCCTT 
TCCAAAATGG GTAGCTCCAT TTTCTCCGGA ATCACTTTAA CCAAATTTGC GGGCATACTA 
GTGTTAGCCT TTGCAAAAAG TCAGATATTC CAGGTGTTTT ATTTCCGTAT GTACTTGGGA 
ATCGTTGTAA TTGGGGCGGC GCACGGGTTG ATCTTTCTGC CCGTTCTATT AAGTTATATT 
GGGGCTCCTG TAAGTAATGC TAGATTAAGG TATCATAGCC AGGCGGCTGC TGAGCACGAG 
ACAGCACTAG CGGGAATACT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188770.1
CDS	137..4000
Translation	MSPRSPLRISPFGVHILIAAVLFTLIQSSKQDCVWYGVCNTNDFSHSQNCPYNGTAKEMATDGLELLKKRCGFLLENSENKFCCDKNQVELLNKNVELAGNILDRCPSCMENLVRHICQFTCSPKQAEFMHVVATQKNKKGDEYISSVDLHISTEYINKTYKSCSQVSVPQTGQLAFDLMCGAYSASRCNPTKWFNFMGDATNPYVPFQITYIQHEPKSNSNNFTPLNVTTVPCNQAVSSKLPACSCSDCDLSCPQGPPEPPRPEPFKIVGLDAYFVIMAAVFLVGVLVFLMGSFLFTQGSSMDDNFQVDGNDVSDEMPYSENDSYFEKLGAHTETFLETFFTKWGTYFASNPGLTLIAGASLVVILGYGINFIEITTDPVKLWASPNSKSRLEREFFDTKFSPFYRLEQIIIKAVNLPQIVHNTSNGPYTFGPVFDREFLTKVLDLQEGIKEINANGTQLKDICYAPLSDDGSEIDVSQCVVQSIWGYFGDDRERLDDHDEDNGFNVTYLDALYDCISNPYLCLAPYGGPVDPAIALGGFLPPGDQLTGSTKFELANAIILTFLVKNHHNKTDLENALTWEKKFVEFMTNYTKNNMSQYMDIAFTSERSIEDELNRESQSDVLTILVSYLIMFMYIAISLGHVKEFKRVFIDSKITLGIGGVIIVLASVVSSVGVFGYIGLPATLIIVEVIPFLVLAVGVDNIFILVQTHQRDQRKPNETLEQQVGRILGKVGPSMLLTSLSESFCFFLGGLSDMPAVRAFALYAGVALIIDFLLQITCFVSLFTLDTKRREENRMDICCFIKGKKPDSITSNEGLLYKFFSSVYVPFLMKKIVRASVMVIFFAWLCFSIAIAPRIDIGLDQELAMPQDSFVLHYFQSLNENLNIGPPVYFVLKGDLAYTNSSDQNLVCAGQYCNDDSVLTQIYLASRHSNQTYIARPASSWIDDYFDWAAAASSCCKYRKDSGDFCPHQDTSCLRCNITKNSLLRPEEKEFVKYLPFFLKDNPDDTCAKAGHAAYGGAVRYSNSHERLNIEASYFMAYHTILKSSADYFLALESARKISANITQMLQGRLMSNGVPMASALTVEVFPYSVFYVFYEQYLTMWSDTLQSMGISVLSIFVVTFVLMGFDVHSALVVVITITMIVVNLGGLMYYWNISLNAVSLVNLVMAVGISVEFCSHLVHSFATSKSVSQIDRAADSLSKMGSSIFSGITLTKFAGILVLAFAKSQIFQVFYFRMYLGIVVIGAAHGLIFLPVLLSYIGAPVSNARLRYHSQAAAEHETALAGIL

Target ORF information:

RefSeq Version	NM_001201841.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201841.2

ORF Insert Sequence:

ATGAGTCCAA GATCCCCGCT CAGGATATCT CCATTTGGAG TACACATCCT CATCGCTGCT 
GTGCTCTTTA CGCTAATTCA ATCATCGAAA CAGGACTGCG TTTGGTATGG CGTGTGCAAC 
ACCAATGACT TTTCGCACAG CCAAAACTGT CCGTACAATG GAACAGCGAA GGAAATGGCA 
ACTGACGGTC TGGAGCTGCT GAAGAAACGC TGTGGATTTC TGCTGGAGAA TAGCGAAAAC 
AAATTTTGCT GCGATAAGAA TCAGGTGGAG CTCCTTAATA AGAATGTGGA GCTGGCCGGA 
AATATCTTGG ATCGATGTCC ATCCTGCATG GAGAACTTGG TGCGGCACAT CTGCCAATTC 
ACCTGTTCAC CAAAGCAGGC TGAGTTCATG CATGTGGTGG CCACGCAGAA AAATAAGAAA 
GGTGATGAGT ACATAAGTTC TGTTGACTTG CATATATCAA CGGAATACAT CAACAAGACT 
TATAAATCGT GCTCTCAGGT TTCTGTACCG CAAACGGGTC AATTAGCTTT TGATTTAATG 
TGCGGCGCTT ACTCAGCATC TCGCTGCAAT CCTACAAAAT GGTTCAACTT TATGGGCGAT 
GCCACCAATC CATATGTTCC TTTTCAAATT ACCTATATTC AACATGAGCC CAAATCAAAT 
AGCAATAACT TTACGCCCCT CAATGTAACG ACCGTTCCTT GCAACCAAGC AGTTAGCAGC 
AAACTACCTG CGTGCTCCTG CTCGGACTGC GACCTATCCT GTCCCCAGGG ACCGCCGGAA 
CCGCCCCGTC CAGAGCCCTT CAAAATTGTT GGACTCGACG CCTATTTCGT CATCATGGCA 
GCTGTCTTCT TGGTGGGAGT CTTAGTATTC TTAATGGGCT CGTTTCTGTT TACACAAGGT 
TCGTCCATGG ATGACAACTT TCAAGTGGAT GGCAATGATG TTTCCGATGA AATGCCCTAC 
AGCGAGAATG ATTCATACTT TGAAAAACTT GGCGCCCACA CAGAAACTTT CCTGGAAACA 
TTCTTCACCA AATGGGGAAC GTACTTTGCT AGCAATCCTG GCTTGACTTT AATTGCCGGA 
GCATCCCTGG TTGTTATACT GGGGTATGGC ATCAACTTTA TAGAAATCAC AACGGATCCG 
GTTAAACTTT GGGCTTCGCC GAACTCAAAA TCCAGACTGG AGAGAGAGTT TTTCGATACG 
AAGTTTTCGC CATTCTATAG GCTGGAACAG ATAATCATCA AAGCTGTGAA CTTGCCACAG 
ATTGTGCACA ACACCTCGAA CGGCCCATAC ACCTTCGGTC CCGTCTTCGA CAGAGAATTT 
CTAACAAAAG TGTTGGATCT GCAGGAGGGC ATCAAGGAAA TTAACGCAAA TGGCACACAG 
CTGAAGGATA TATGCTACGC GCCTTTGTCG GATGATGGCA GTGAAATCGA TGTGTCCCAG 
TGCGTTGTCC AATCGATTTG GGGATACTTT GGCGATGATC GAGAAAGATT GGATGACCAC 
GACGAGGACA ATGGATTCAA TGTTACCTAT TTGGATGCCT TATACGATTG CATTAGCAAT 
CCCTATTTAT GCCTAGCTCC TTATGGCGGT CCCGTAGATC CAGCCATAGC ACTTGGAGGA 
TTCCTGCCAC CTGGAGACCA ACTGACTGGC AGCACGAAAT TTGAGTTGGC CAATGCGATC 
ATTCTGACAT TCCTGGTCAA GAATCATCAT AACAAAACCG ATTTGGAAAA TGCCTTAACG 
TGGGAGAAGA AATTCGTGGA GTTTATGACC AACTATACGA AGAACAATAT GTCGCAGTAC 
ATGGACATAG CCTTCACATC GGAGAGATCG ATAGAAGACG AGCTCAACAG GGAGTCCCAG 
TCGGATGTGC TGACTATTTT GGTGTCCTAC CTAATTATGT TCATGTACAT AGCGATTTCG 
CTTGGCCATG TCAAGGAGTT CAAGCGAGTG TTTATCGACA GTAAGATAAC GCTTGGCATA 
GGCGGTGTCA TCATTGTTTT GGCCTCGGTA GTGTCGTCCG TTGGCGTTTT CGGTTACATC 
GGTTTGCCGG CCACCCTGAT CATTGTCGAG GTCATACCGT TTTTAGTATT AGCTGTCGGA 
GTGGATAACA TTTTTATTCT GGTCCAAACT CATCAGCGCG ATCAACGCAA ACCCAATGAA 
ACGCTTGAGC AGCAAGTTGG TCGAATACTC GGCAAGGTTG GACCCAGTAT GCTGCTCACC 
TCGTTGAGTG AAAGCTTTTG CTTCTTTCTC GGTGGCCTTT CTGATATGCC AGCCGTAAGG 
GCTTTTGCCC TATACGCCGG CGTAGCCCTC ATCATTGATT TTCTATTGCA AATAACCTGT 
TTCGTTAGCC TATTTACGTT GGATACGAAG CGCAGGGAGG AGAATCGTAT GGACATTTGT 
TGTTTCATCA AGGGCAAGAA GCCGGATAGT ATAACTAGTA ATGAGGGACT CTTGTACAAA 
TTTTTCAGTA GTGTTTACGT CCCATTTCTG ATGAAGAAAA TTGTGCGCGC CAGTGTGATG 
GTCATCTTTT TTGCCTGGCT GTGCTTCAGC ATTGCCATTG CGCCACGAAT CGACATTGGG 
CTGGATCAAG AGTTGGCTAT GCCGCAGGAT AGTTTCGTCT TGCACTATTT CCAATCGTTG 
AACGAGAATC TCAACATTGG TCCACCAGTG TACTTTGTGC TGAAGGGCGA TCTAGCCTAT 
ACGAATAGTT CGGATCAGAA TTTGGTGTGT GCAGGTCAAT ATTGCAACGA TGACAGCGTA 
CTCACGCAGA TCTACTTAGC CAGTAGGCAC TCTAACCAAA CGTATATCGC GCGTCCAGCA 
TCCAGTTGGA TCGATGACTA CTTCGATTGG GCGGCAGCAG CATCATCCTG TTGCAAATAT 
AGAAAGGATA GCGGCGACTT TTGTCCGCAT CAGGATACTT CCTGTTTGAG GTGTAATATC 
ACGAAGAATT CACTTCTTAG GCCGGAGGAA AAGGAGTTTG TTAAGTATTT GCCATTTTTC 
CTGAAGGACA ATCCCGATGA CACGTGCGCC AAGGCCGGTC ATGCCGCCTA TGGCGGAGCT 
GTTCGGTATT CCAACAGCCA TGAAAGACTG AACATCGAGG CGTCGTATTT TATGGCATAC 
CACACCATAC TTAAGTCCTC GGCCGACTAC TTTCTGGCCC TAGAGTCGGC TAGAAAGATA 
TCCGCGAATA TTACGCAAAT GTTGCAGGGC AGACTAATGT CAAATGGAGT TCCTATGGCA 
TCGGCCCTTA CGGTCGAAGT GTTTCCATAT TCAGTATTCT ATGTGTTTTA CGAACAATAT 
CTGACCATGT GGTCGGATAC GCTACAGAGC ATGGGTATTT CAGTTCTTTC CATATTCGTT 
GTTACATTTG TTTTGATGGG CTTCGATGTT CATTCCGCAT TAGTTGTTGT CATAACGATA 
ACTATGATTG TTGTTAATTT AGGTGGCCTT ATGTATTATT GGAACATTTC GTTGAATGCT 
GTTTCGTTGG TGAATCTCGT CATGGCCGTC GGCATATCCG TGGAATTTTG TTCGCACTTG 
GTGCACAGCT TCGCCACATC GAAATCAGTT AGTCAAATTG ATCGAGCTGC AGATAGCCTT 
TCCAAAATGG GTAGCTCCAT TTTCTCCGGA ATCACTTTAA CCAAATTTGC GGGCATACTA 
GTGTTAGCCT TTGCAAAAAG TCAGATATTC CAGGTGTTTT ATTTCCGTAT GTACTTGGGA 
ATCGTTGTAA TTGGGGCGGC GCACGGGTTG ATCTTTCTGC CCGTTCTATT AAGTTATATT 
GGGGCTCCTG TAAGTAATGC TAGATTAAGG TATCATAGCC AGGCGGCTGC TGAGCACGAG 
ACAGCACTAG CGGGAATACT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npc1a ( NM_135513.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609357.2 Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14284
Clone ID Related Accession (Same CDS sequence)	NM_001201841.2 , NM_135513.5 , NM_001201840.2 , NM_001201842.2
Accession Version	NM_135513.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Niemann-Pick type C-1a, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135513.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCCAA GATCCCCGCT CAGGATATCT CCATTTGGAG TACACATCCT CATCGCTGCT 
GTGCTCTTTA CGCTAATTCA ATCATCGAAA CAGGACTGCG TTTGGTATGG CGTGTGCAAC 
ACCAATGACT TTTCGCACAG CCAAAACTGT CCGTACAATG GAACAGCGAA GGAAATGGCA 
ACTGACGGTC TGGAGCTGCT GAAGAAACGC TGTGGATTTC TGCTGGAGAA TAGCGAAAAC 
AAATTTTGCT GCGATAAGAA TCAGGTGGAG CTCCTTAATA AGAATGTGGA GCTGGCCGGA 
AATATCTTGG ATCGATGTCC ATCCTGCATG GAGAACTTGG TGCGGCACAT CTGCCAATTC 
ACCTGTTCAC CAAAGCAGGC TGAGTTCATG CATGTGGTGG CCACGCAGAA AAATAAGAAA 
GGTGATGAGT ACATAAGTTC TGTTGACTTG CATATATCAA CGGAATACAT CAACAAGACT 
TATAAATCGT GCTCTCAGGT TTCTGTACCG CAAACGGGTC AATTAGCTTT TGATTTAATG 
TGCGGCGCTT ACTCAGCATC TCGCTGCAAT CCTACAAAAT GGTTCAACTT TATGGGCGAT 
GCCACCAATC CATATGTTCC TTTTCAAATT ACCTATATTC AACATGAGCC CAAATCAAAT 
AGCAATAACT TTACGCCCCT CAATGTAACG ACCGTTCCTT GCAACCAAGC AGTTAGCAGC 
AAACTACCTG CGTGCTCCTG CTCGGACTGC GACCTATCCT GTCCCCAGGG ACCGCCGGAA 
CCGCCCCGTC CAGAGCCCTT CAAAATTGTT GGACTCGACG CCTATTTCGT CATCATGGCA 
GCTGTCTTCT TGGTGGGAGT CTTAGTATTC TTAATGGGCT CGTTTCTGTT TACACAAGGT 
TCGTCCATGG ATGACAACTT TCAAGTGGAT GGCAATGATG TTTCCGATGA AATGCCCTAC 
AGCGAGAATG ATTCATACTT TGAAAAACTT GGCGCCCACA CAGAAACTTT CCTGGAAACA 
TTCTTCACCA AATGGGGAAC GTACTTTGCT AGCAATCCTG GCTTGACTTT AATTGCCGGA 
GCATCCCTGG TTGTTATACT GGGGTATGGC ATCAACTTTA TAGAAATCAC AACGGATCCG 
GTTAAACTTT GGGCTTCGCC GAACTCAAAA TCCAGACTGG AGAGAGAGTT TTTCGATACG 
AAGTTTTCGC CATTCTATAG GCTGGAACAG ATAATCATCA AAGCTGTGAA CTTGCCACAG 
ATTGTGCACA ACACCTCGAA CGGCCCATAC ACCTTCGGTC CCGTCTTCGA CAGAGAATTT 
CTAACAAAAG TGTTGGATCT GCAGGAGGGC ATCAAGGAAA TTAACGCAAA TGGCACACAG 
CTGAAGGATA TATGCTACGC GCCTTTGTCG GATGATGGCA GTGAAATCGA TGTGTCCCAG 
TGCGTTGTCC AATCGATTTG GGGATACTTT GGCGATGATC GAGAAAGATT GGATGACCAC 
GACGAGGACA ATGGATTCAA TGTTACCTAT TTGGATGCCT TATACGATTG CATTAGCAAT 
CCCTATTTAT GCCTAGCTCC TTATGGCGGT CCCGTAGATC CAGCCATAGC ACTTGGAGGA 
TTCCTGCCAC CTGGAGACCA ACTGACTGGC AGCACGAAAT TTGAGTTGGC CAATGCGATC 
ATTCTGACAT TCCTGGTCAA GAATCATCAT AACAAAACCG ATTTGGAAAA TGCCTTAACG 
TGGGAGAAGA AATTCGTGGA GTTTATGACC AACTATACGA AGAACAATAT GTCGCAGTAC 
ATGGACATAG CCTTCACATC GGAGAGATCG ATAGAAGACG AGCTCAACAG GGAGTCCCAG 
TCGGATGTGC TGACTATTTT GGTGTCCTAC CTAATTATGT TCATGTACAT AGCGATTTCG 
CTTGGCCATG TCAAGGAGTT CAAGCGAGTG TTTATCGACA GTAAGATAAC GCTTGGCATA 
GGCGGTGTCA TCATTGTTTT GGCCTCGGTA GTGTCGTCCG TTGGCGTTTT CGGTTACATC 
GGTTTGCCGG CCACCCTGAT CATTGTCGAG GTCATACCGT TTTTAGTATT AGCTGTCGGA 
GTGGATAACA TTTTTATTCT GGTCCAAACT CATCAGCGCG ATCAACGCAA ACCCAATGAA 
ACGCTTGAGC AGCAAGTTGG TCGAATACTC GGCAAGGTTG GACCCAGTAT GCTGCTCACC 
TCGTTGAGTG AAAGCTTTTG CTTCTTTCTC GGTGGCCTTT CTGATATGCC AGCCGTAAGG 
GCTTTTGCCC TATACGCCGG CGTAGCCCTC ATCATTGATT TTCTATTGCA AATAACCTGT 
TTCGTTAGCC TATTTACGTT GGATACGAAG CGCAGGGAGG AGAATCGTAT GGACATTTGT 
TGTTTCATCA AGGGCAAGAA GCCGGATAGT ATAACTAGTA ATGAGGGACT CTTGTACAAA 
TTTTTCAGTA GTGTTTACGT CCCATTTCTG ATGAAGAAAA TTGTGCGCGC CAGTGTGATG 
GTCATCTTTT TTGCCTGGCT GTGCTTCAGC ATTGCCATTG CGCCACGAAT CGACATTGGG 
CTGGATCAAG AGTTGGCTAT GCCGCAGGAT AGTTTCGTCT TGCACTATTT CCAATCGTTG 
AACGAGAATC TCAACATTGG TCCACCAGTG TACTTTGTGC TGAAGGGCGA TCTAGCCTAT 
ACGAATAGTT CGGATCAGAA TTTGGTGTGT GCAGGTCAAT ATTGCAACGA TGACAGCGTA 
CTCACGCAGA TCTACTTAGC CAGTAGGCAC TCTAACCAAA CGTATATCGC GCGTCCAGCA 
TCCAGTTGGA TCGATGACTA CTTCGATTGG GCGGCAGCAG CATCATCCTG TTGCAAATAT 
AGAAAGGATA GCGGCGACTT TTGTCCGCAT CAGGATACTT CCTGTTTGAG GTGTAATATC 
ACGAAGAATT CACTTCTTAG GCCGGAGGAA AAGGAGTTTG TTAAGTATTT GCCATTTTTC 
CTGAAGGACA ATCCCGATGA CACGTGCGCC AAGGCCGGTC ATGCCGCCTA TGGCGGAGCT 
GTTCGGTATT CCAACAGCCA TGAAAGACTG AACATCGAGG CGTCGTATTT TATGGCATAC 
CACACCATAC TTAAGTCCTC GGCCGACTAC TTTCTGGCCC TAGAGTCGGC TAGAAAGATA 
TCCGCGAATA TTACGCAAAT GTTGCAGGGC AGACTAATGT CAAATGGAGT TCCTATGGCA 
TCGGCCCTTA CGGTCGAAGT GTTTCCATAT TCAGTATTCT ATGTGTTTTA CGAACAATAT 
CTGACCATGT GGTCGGATAC GCTACAGAGC ATGGGTATTT CAGTTCTTTC CATATTCGTT 
GTTACATTTG TTTTGATGGG CTTCGATGTT CATTCCGCAT TAGTTGTTGT CATAACGATA 
ACTATGATTG TTGTTAATTT AGGTGGCCTT ATGTATTATT GGAACATTTC GTTGAATGCT 
GTTTCGTTGG TGAATCTCGT CATGGCCGTC GGCATATCCG TGGAATTTTG TTCGCACTTG 
GTGCACAGCT TCGCCACATC GAAATCAGTT AGTCAAATTG ATCGAGCTGC AGATAGCCTT 
TCCAAAATGG GTAGCTCCAT TTTCTCCGGA ATCACTTTAA CCAAATTTGC GGGCATACTA 
GTGTTAGCCT TTGCAAAAAG TCAGATATTC CAGGTGTTTT ATTTCCGTAT GTACTTGGGA 
ATCGTTGTAA TTGGGGCGGC GCACGGGTTG ATCTTTCTGC CCGTTCTATT AAGTTATATT 
GGGGCTCCTG TAAGTAATGC TAGATTAAGG TATCATAGCC AGGCGGCTGC TGAGCACGAG 
ACAGCACTAG CGGGAATACT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609357.2
CDS	279..4142
Translation	MSPRSPLRISPFGVHILIAAVLFTLIQSSKQDCVWYGVCNTNDFSHSQNCPYNGTAKEMATDGLELLKKRCGFLLENSENKFCCDKNQVELLNKNVELAGNILDRCPSCMENLVRHICQFTCSPKQAEFMHVVATQKNKKGDEYISSVDLHISTEYINKTYKSCSQVSVPQTGQLAFDLMCGAYSASRCNPTKWFNFMGDATNPYVPFQITYIQHEPKSNSNNFTPLNVTTVPCNQAVSSKLPACSCSDCDLSCPQGPPEPPRPEPFKIVGLDAYFVIMAAVFLVGVLVFLMGSFLFTQGSSMDDNFQVDGNDVSDEMPYSENDSYFEKLGAHTETFLETFFTKWGTYFASNPGLTLIAGASLVVILGYGINFIEITTDPVKLWASPNSKSRLEREFFDTKFSPFYRLEQIIIKAVNLPQIVHNTSNGPYTFGPVFDREFLTKVLDLQEGIKEINANGTQLKDICYAPLSDDGSEIDVSQCVVQSIWGYFGDDRERLDDHDEDNGFNVTYLDALYDCISNPYLCLAPYGGPVDPAIALGGFLPPGDQLTGSTKFELANAIILTFLVKNHHNKTDLENALTWEKKFVEFMTNYTKNNMSQYMDIAFTSERSIEDELNRESQSDVLTILVSYLIMFMYIAISLGHVKEFKRVFIDSKITLGIGGVIIVLASVVSSVGVFGYIGLPATLIIVEVIPFLVLAVGVDNIFILVQTHQRDQRKPNETLEQQVGRILGKVGPSMLLTSLSESFCFFLGGLSDMPAVRAFALYAGVALIIDFLLQITCFVSLFTLDTKRREENRMDICCFIKGKKPDSITSNEGLLYKFFSSVYVPFLMKKIVRASVMVIFFAWLCFSIAIAPRIDIGLDQELAMPQDSFVLHYFQSLNENLNIGPPVYFVLKGDLAYTNSSDQNLVCAGQYCNDDSVLTQIYLASRHSNQTYIARPASSWIDDYFDWAAAASSCCKYRKDSGDFCPHQDTSCLRCNITKNSLLRPEEKEFVKYLPFFLKDNPDDTCAKAGHAAYGGAVRYSNSHERLNIEASYFMAYHTILKSSADYFLALESARKISANITQMLQGRLMSNGVPMASALTVEVFPYSVFYVFYEQYLTMWSDTLQSMGISVLSIFVVTFVLMGFDVHSALVVVITITMIVVNLGGLMYYWNISLNAVSLVNLVMAVGISVEFCSHLVHSFATSKSVSQIDRAADSLSKMGSSIFSGITLTKFAGILVLAFAKSQIFQVFYFRMYLGIVVIGAAHGLIFLPVLLSYIGAPVSNARLRYHSQAAAEHETALAGIL

Target ORF information:

RefSeq Version	NM_135513.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135513.5

ORF Insert Sequence:

ATGAGTCCAA GATCCCCGCT CAGGATATCT CCATTTGGAG TACACATCCT CATCGCTGCT 
GTGCTCTTTA CGCTAATTCA ATCATCGAAA CAGGACTGCG TTTGGTATGG CGTGTGCAAC 
ACCAATGACT TTTCGCACAG CCAAAACTGT CCGTACAATG GAACAGCGAA GGAAATGGCA 
ACTGACGGTC TGGAGCTGCT GAAGAAACGC TGTGGATTTC TGCTGGAGAA TAGCGAAAAC 
AAATTTTGCT GCGATAAGAA TCAGGTGGAG CTCCTTAATA AGAATGTGGA GCTGGCCGGA 
AATATCTTGG ATCGATGTCC ATCCTGCATG GAGAACTTGG TGCGGCACAT CTGCCAATTC 
ACCTGTTCAC CAAAGCAGGC TGAGTTCATG CATGTGGTGG CCACGCAGAA AAATAAGAAA 
GGTGATGAGT ACATAAGTTC TGTTGACTTG CATATATCAA CGGAATACAT CAACAAGACT 
TATAAATCGT GCTCTCAGGT TTCTGTACCG CAAACGGGTC AATTAGCTTT TGATTTAATG 
TGCGGCGCTT ACTCAGCATC TCGCTGCAAT CCTACAAAAT GGTTCAACTT TATGGGCGAT 
GCCACCAATC CATATGTTCC TTTTCAAATT ACCTATATTC AACATGAGCC CAAATCAAAT 
AGCAATAACT TTACGCCCCT CAATGTAACG ACCGTTCCTT GCAACCAAGC AGTTAGCAGC 
AAACTACCTG CGTGCTCCTG CTCGGACTGC GACCTATCCT GTCCCCAGGG ACCGCCGGAA 
CCGCCCCGTC CAGAGCCCTT CAAAATTGTT GGACTCGACG CCTATTTCGT CATCATGGCA 
GCTGTCTTCT TGGTGGGAGT CTTAGTATTC TTAATGGGCT CGTTTCTGTT TACACAAGGT 
TCGTCCATGG ATGACAACTT TCAAGTGGAT GGCAATGATG TTTCCGATGA AATGCCCTAC 
AGCGAGAATG ATTCATACTT TGAAAAACTT GGCGCCCACA CAGAAACTTT CCTGGAAACA 
TTCTTCACCA AATGGGGAAC GTACTTTGCT AGCAATCCTG GCTTGACTTT AATTGCCGGA 
GCATCCCTGG TTGTTATACT GGGGTATGGC ATCAACTTTA TAGAAATCAC AACGGATCCG 
GTTAAACTTT GGGCTTCGCC GAACTCAAAA TCCAGACTGG AGAGAGAGTT TTTCGATACG 
AAGTTTTCGC CATTCTATAG GCTGGAACAG ATAATCATCA AAGCTGTGAA CTTGCCACAG 
ATTGTGCACA ACACCTCGAA CGGCCCATAC ACCTTCGGTC CCGTCTTCGA CAGAGAATTT 
CTAACAAAAG TGTTGGATCT GCAGGAGGGC ATCAAGGAAA TTAACGCAAA TGGCACACAG 
CTGAAGGATA TATGCTACGC GCCTTTGTCG GATGATGGCA GTGAAATCGA TGTGTCCCAG 
TGCGTTGTCC AATCGATTTG GGGATACTTT GGCGATGATC GAGAAAGATT GGATGACCAC 
GACGAGGACA ATGGATTCAA TGTTACCTAT TTGGATGCCT TATACGATTG CATTAGCAAT 
CCCTATTTAT GCCTAGCTCC TTATGGCGGT CCCGTAGATC CAGCCATAGC ACTTGGAGGA 
TTCCTGCCAC CTGGAGACCA ACTGACTGGC AGCACGAAAT TTGAGTTGGC CAATGCGATC 
ATTCTGACAT TCCTGGTCAA GAATCATCAT AACAAAACCG ATTTGGAAAA TGCCTTAACG 
TGGGAGAAGA AATTCGTGGA GTTTATGACC AACTATACGA AGAACAATAT GTCGCAGTAC 
ATGGACATAG CCTTCACATC GGAGAGATCG ATAGAAGACG AGCTCAACAG GGAGTCCCAG 
TCGGATGTGC TGACTATTTT GGTGTCCTAC CTAATTATGT TCATGTACAT AGCGATTTCG 
CTTGGCCATG TCAAGGAGTT CAAGCGAGTG TTTATCGACA GTAAGATAAC GCTTGGCATA 
GGCGGTGTCA TCATTGTTTT GGCCTCGGTA GTGTCGTCCG TTGGCGTTTT CGGTTACATC 
GGTTTGCCGG CCACCCTGAT CATTGTCGAG GTCATACCGT TTTTAGTATT AGCTGTCGGA 
GTGGATAACA TTTTTATTCT GGTCCAAACT CATCAGCGCG ATCAACGCAA ACCCAATGAA 
ACGCTTGAGC AGCAAGTTGG TCGAATACTC GGCAAGGTTG GACCCAGTAT GCTGCTCACC 
TCGTTGAGTG AAAGCTTTTG CTTCTTTCTC GGTGGCCTTT CTGATATGCC AGCCGTAAGG 
GCTTTTGCCC TATACGCCGG CGTAGCCCTC ATCATTGATT TTCTATTGCA AATAACCTGT 
TTCGTTAGCC TATTTACGTT GGATACGAAG CGCAGGGAGG AGAATCGTAT GGACATTTGT 
TGTTTCATCA AGGGCAAGAA GCCGGATAGT ATAACTAGTA ATGAGGGACT CTTGTACAAA 
TTTTTCAGTA GTGTTTACGT CCCATTTCTG ATGAAGAAAA TTGTGCGCGC CAGTGTGATG 
GTCATCTTTT TTGCCTGGCT GTGCTTCAGC ATTGCCATTG CGCCACGAAT CGACATTGGG 
CTGGATCAAG AGTTGGCTAT GCCGCAGGAT AGTTTCGTCT TGCACTATTT CCAATCGTTG 
AACGAGAATC TCAACATTGG TCCACCAGTG TACTTTGTGC TGAAGGGCGA TCTAGCCTAT 
ACGAATAGTT CGGATCAGAA TTTGGTGTGT GCAGGTCAAT ATTGCAACGA TGACAGCGTA 
CTCACGCAGA TCTACTTAGC CAGTAGGCAC TCTAACCAAA CGTATATCGC GCGTCCAGCA 
TCCAGTTGGA TCGATGACTA CTTCGATTGG GCGGCAGCAG CATCATCCTG TTGCAAATAT 
AGAAAGGATA GCGGCGACTT TTGTCCGCAT CAGGATACTT CCTGTTTGAG GTGTAATATC 
ACGAAGAATT CACTTCTTAG GCCGGAGGAA AAGGAGTTTG TTAAGTATTT GCCATTTTTC 
CTGAAGGACA ATCCCGATGA CACGTGCGCC AAGGCCGGTC ATGCCGCCTA TGGCGGAGCT 
GTTCGGTATT CCAACAGCCA TGAAAGACTG AACATCGAGG CGTCGTATTT TATGGCATAC 
CACACCATAC TTAAGTCCTC GGCCGACTAC TTTCTGGCCC TAGAGTCGGC TAGAAAGATA 
TCCGCGAATA TTACGCAAAT GTTGCAGGGC AGACTAATGT CAAATGGAGT TCCTATGGCA 
TCGGCCCTTA CGGTCGAAGT GTTTCCATAT TCAGTATTCT ATGTGTTTTA CGAACAATAT 
CTGACCATGT GGTCGGATAC GCTACAGAGC ATGGGTATTT CAGTTCTTTC CATATTCGTT 
GTTACATTTG TTTTGATGGG CTTCGATGTT CATTCCGCAT TAGTTGTTGT CATAACGATA 
ACTATGATTG TTGTTAATTT AGGTGGCCTT ATGTATTATT GGAACATTTC GTTGAATGCT 
GTTTCGTTGG TGAATCTCGT CATGGCCGTC GGCATATCCG TGGAATTTTG TTCGCACTTG 
GTGCACAGCT TCGCCACATC GAAATCAGTT AGTCAAATTG ATCGAGCTGC AGATAGCCTT 
TCCAAAATGG GTAGCTCCAT TTTCTCCGGA ATCACTTTAA CCAAATTTGC GGGCATACTA 
GTGTTAGCCT TTGCAAAAAG TCAGATATTC CAGGTGTTTT ATTTCCGTAT GTACTTGGGA 
ATCGTTGTAA TTGGGGCGGC GCACGGGTTG ATCTTTCTGC CCGTTCTATT AAGTTATATT 
GGGGCTCCTG TAAGTAATGC TAGATTAAGG TATCATAGCC AGGCGGCTGC TGAGCACGAG 
ACAGCACTAG CGGGAATACT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npc1a ( NM_001201840.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188769.1 Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant B, mRNA.

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CloneID	OFa14284
Clone ID Related Accession (Same CDS sequence)	NM_001201841.2 , NM_135513.5 , NM_001201840.2 , NM_001201842.2
Accession Version	NM_001201840.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Niemann-Pick type C-1a, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001201840.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCCAA GATCCCCGCT CAGGATATCT CCATTTGGAG TACACATCCT CATCGCTGCT 
GTGCTCTTTA CGCTAATTCA ATCATCGAAA CAGGACTGCG TTTGGTATGG CGTGTGCAAC 
ACCAATGACT TTTCGCACAG CCAAAACTGT CCGTACAATG GAACAGCGAA GGAAATGGCA 
ACTGACGGTC TGGAGCTGCT GAAGAAACGC TGTGGATTTC TGCTGGAGAA TAGCGAAAAC 
AAATTTTGCT GCGATAAGAA TCAGGTGGAG CTCCTTAATA AGAATGTGGA GCTGGCCGGA 
AATATCTTGG ATCGATGTCC ATCCTGCATG GAGAACTTGG TGCGGCACAT CTGCCAATTC 
ACCTGTTCAC CAAAGCAGGC TGAGTTCATG CATGTGGTGG CCACGCAGAA AAATAAGAAA 
GGTGATGAGT ACATAAGTTC TGTTGACTTG CATATATCAA CGGAATACAT CAACAAGACT 
TATAAATCGT GCTCTCAGGT TTCTGTACCG CAAACGGGTC AATTAGCTTT TGATTTAATG 
TGCGGCGCTT ACTCAGCATC TCGCTGCAAT CCTACAAAAT GGTTCAACTT TATGGGCGAT 
GCCACCAATC CATATGTTCC TTTTCAAATT ACCTATATTC AACATGAGCC CAAATCAAAT 
AGCAATAACT TTACGCCCCT CAATGTAACG ACCGTTCCTT GCAACCAAGC AGTTAGCAGC 
AAACTACCTG CGTGCTCCTG CTCGGACTGC GACCTATCCT GTCCCCAGGG ACCGCCGGAA 
CCGCCCCGTC CAGAGCCCTT CAAAATTGTT GGACTCGACG CCTATTTCGT CATCATGGCA 
GCTGTCTTCT TGGTGGGAGT CTTAGTATTC TTAATGGGCT CGTTTCTGTT TACACAAGGT 
TCGTCCATGG ATGACAACTT TCAAGTGGAT GGCAATGATG TTTCCGATGA AATGCCCTAC 
AGCGAGAATG ATTCATACTT TGAAAAACTT GGCGCCCACA CAGAAACTTT CCTGGAAACA 
TTCTTCACCA AATGGGGAAC GTACTTTGCT AGCAATCCTG GCTTGACTTT AATTGCCGGA 
GCATCCCTGG TTGTTATACT GGGGTATGGC ATCAACTTTA TAGAAATCAC AACGGATCCG 
GTTAAACTTT GGGCTTCGCC GAACTCAAAA TCCAGACTGG AGAGAGAGTT TTTCGATACG 
AAGTTTTCGC CATTCTATAG GCTGGAACAG ATAATCATCA AAGCTGTGAA CTTGCCACAG 
ATTGTGCACA ACACCTCGAA CGGCCCATAC ACCTTCGGTC CCGTCTTCGA CAGAGAATTT 
CTAACAAAAG TGTTGGATCT GCAGGAGGGC ATCAAGGAAA TTAACGCAAA TGGCACACAG 
CTGAAGGATA TATGCTACGC GCCTTTGTCG GATGATGGCA GTGAAATCGA TGTGTCCCAG 
TGCGTTGTCC AATCGATTTG GGGATACTTT GGCGATGATC GAGAAAGATT GGATGACCAC 
GACGAGGACA ATGGATTCAA TGTTACCTAT TTGGATGCCT TATACGATTG CATTAGCAAT 
CCCTATTTAT GCCTAGCTCC TTATGGCGGT CCCGTAGATC CAGCCATAGC ACTTGGAGGA 
TTCCTGCCAC CTGGAGACCA ACTGACTGGC AGCACGAAAT TTGAGTTGGC CAATGCGATC 
ATTCTGACAT TCCTGGTCAA GAATCATCAT AACAAAACCG ATTTGGAAAA TGCCTTAACG 
TGGGAGAAGA AATTCGTGGA GTTTATGACC AACTATACGA AGAACAATAT GTCGCAGTAC 
ATGGACATAG CCTTCACATC GGAGAGATCG ATAGAAGACG AGCTCAACAG GGAGTCCCAG 
TCGGATGTGC TGACTATTTT GGTGTCCTAC CTAATTATGT TCATGTACAT AGCGATTTCG 
CTTGGCCATG TCAAGGAGTT CAAGCGAGTG TTTATCGACA GTAAGATAAC GCTTGGCATA 
GGCGGTGTCA TCATTGTTTT GGCCTCGGTA GTGTCGTCCG TTGGCGTTTT CGGTTACATC 
GGTTTGCCGG CCACCCTGAT CATTGTCGAG GTCATACCGT TTTTAGTATT AGCTGTCGGA 
GTGGATAACA TTTTTATTCT GGTCCAAACT CATCAGCGCG ATCAACGCAA ACCCAATGAA 
ACGCTTGAGC AGCAAGTTGG TCGAATACTC GGCAAGGTTG GACCCAGTAT GCTGCTCACC 
TCGTTGAGTG AAAGCTTTTG CTTCTTTCTC GGTGGCCTTT CTGATATGCC AGCCGTAAGG 
GCTTTTGCCC TATACGCCGG CGTAGCCCTC ATCATTGATT TTCTATTGCA AATAACCTGT 
TTCGTTAGCC TATTTACGTT GGATACGAAG CGCAGGGAGG AGAATCGTAT GGACATTTGT 
TGTTTCATCA AGGGCAAGAA GCCGGATAGT ATAACTAGTA ATGAGGGACT CTTGTACAAA 
TTTTTCAGTA GTGTTTACGT CCCATTTCTG ATGAAGAAAA TTGTGCGCGC CAGTGTGATG 
GTCATCTTTT TTGCCTGGCT GTGCTTCAGC ATTGCCATTG CGCCACGAAT CGACATTGGG 
CTGGATCAAG AGTTGGCTAT GCCGCAGGAT AGTTTCGTCT TGCACTATTT CCAATCGTTG 
AACGAGAATC TCAACATTGG TCCACCAGTG TACTTTGTGC TGAAGGGCGA TCTAGCCTAT 
ACGAATAGTT CGGATCAGAA TTTGGTGTGT GCAGGTCAAT ATTGCAACGA TGACAGCGTA 
CTCACGCAGA TCTACTTAGC CAGTAGGCAC TCTAACCAAA CGTATATCGC GCGTCCAGCA 
TCCAGTTGGA TCGATGACTA CTTCGATTGG GCGGCAGCAG CATCATCCTG TTGCAAATAT 
AGAAAGGATA GCGGCGACTT TTGTCCGCAT CAGGATACTT CCTGTTTGAG GTGTAATATC 
ACGAAGAATT CACTTCTTAG GCCGGAGGAA AAGGAGTTTG TTAAGTATTT GCCATTTTTC 
CTGAAGGACA ATCCCGATGA CACGTGCGCC AAGGCCGGTC ATGCCGCCTA TGGCGGAGCT 
GTTCGGTATT CCAACAGCCA TGAAAGACTG AACATCGAGG CGTCGTATTT TATGGCATAC 
CACACCATAC TTAAGTCCTC GGCCGACTAC TTTCTGGCCC TAGAGTCGGC TAGAAAGATA 
TCCGCGAATA TTACGCAAAT GTTGCAGGGC AGACTAATGT CAAATGGAGT TCCTATGGCA 
TCGGCCCTTA CGGTCGAAGT GTTTCCATAT TCAGTATTCT ATGTGTTTTA CGAACAATAT 
CTGACCATGT GGTCGGATAC GCTACAGAGC ATGGGTATTT CAGTTCTTTC CATATTCGTT 
GTTACATTTG TTTTGATGGG CTTCGATGTT CATTCCGCAT TAGTTGTTGT CATAACGATA 
ACTATGATTG TTGTTAATTT AGGTGGCCTT ATGTATTATT GGAACATTTC GTTGAATGCT 
GTTTCGTTGG TGAATCTCGT CATGGCCGTC GGCATATCCG TGGAATTTTG TTCGCACTTG 
GTGCACAGCT TCGCCACATC GAAATCAGTT AGTCAAATTG ATCGAGCTGC AGATAGCCTT 
TCCAAAATGG GTAGCTCCAT TTTCTCCGGA ATCACTTTAA CCAAATTTGC GGGCATACTA 
GTGTTAGCCT TTGCAAAAAG TCAGATATTC CAGGTGTTTT ATTTCCGTAT GTACTTGGGA 
ATCGTTGTAA TTGGGGCGGC GCACGGGTTG ATCTTTCTGC CCGTTCTATT AAGTTATATT 
GGGGCTCCTG TAAGTAATGC TAGATTAAGG TATCATAGCC AGGCGGCTGC TGAGCACGAG 
ACAGCACTAG CGGGAATACT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188769.1
CDS	175..4038
Translation	MSPRSPLRISPFGVHILIAAVLFTLIQSSKQDCVWYGVCNTNDFSHSQNCPYNGTAKEMATDGLELLKKRCGFLLENSENKFCCDKNQVELLNKNVELAGNILDRCPSCMENLVRHICQFTCSPKQAEFMHVVATQKNKKGDEYISSVDLHISTEYINKTYKSCSQVSVPQTGQLAFDLMCGAYSASRCNPTKWFNFMGDATNPYVPFQITYIQHEPKSNSNNFTPLNVTTVPCNQAVSSKLPACSCSDCDLSCPQGPPEPPRPEPFKIVGLDAYFVIMAAVFLVGVLVFLMGSFLFTQGSSMDDNFQVDGNDVSDEMPYSENDSYFEKLGAHTETFLETFFTKWGTYFASNPGLTLIAGASLVVILGYGINFIEITTDPVKLWASPNSKSRLEREFFDTKFSPFYRLEQIIIKAVNLPQIVHNTSNGPYTFGPVFDREFLTKVLDLQEGIKEINANGTQLKDICYAPLSDDGSEIDVSQCVVQSIWGYFGDDRERLDDHDEDNGFNVTYLDALYDCISNPYLCLAPYGGPVDPAIALGGFLPPGDQLTGSTKFELANAIILTFLVKNHHNKTDLENALTWEKKFVEFMTNYTKNNMSQYMDIAFTSERSIEDELNRESQSDVLTILVSYLIMFMYIAISLGHVKEFKRVFIDSKITLGIGGVIIVLASVVSSVGVFGYIGLPATLIIVEVIPFLVLAVGVDNIFILVQTHQRDQRKPNETLEQQVGRILGKVGPSMLLTSLSESFCFFLGGLSDMPAVRAFALYAGVALIIDFLLQITCFVSLFTLDTKRREENRMDICCFIKGKKPDSITSNEGLLYKFFSSVYVPFLMKKIVRASVMVIFFAWLCFSIAIAPRIDIGLDQELAMPQDSFVLHYFQSLNENLNIGPPVYFVLKGDLAYTNSSDQNLVCAGQYCNDDSVLTQIYLASRHSNQTYIARPASSWIDDYFDWAAAASSCCKYRKDSGDFCPHQDTSCLRCNITKNSLLRPEEKEFVKYLPFFLKDNPDDTCAKAGHAAYGGAVRYSNSHERLNIEASYFMAYHTILKSSADYFLALESARKISANITQMLQGRLMSNGVPMASALTVEVFPYSVFYVFYEQYLTMWSDTLQSMGISVLSIFVVTFVLMGFDVHSALVVVITITMIVVNLGGLMYYWNISLNAVSLVNLVMAVGISVEFCSHLVHSFATSKSVSQIDRAADSLSKMGSSIFSGITLTKFAGILVLAFAKSQIFQVFYFRMYLGIVVIGAAHGLIFLPVLLSYIGAPVSNARLRYHSQAAAEHETALAGIL

Target ORF information:

RefSeq Version	NM_001201840.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201840.2

ORF Insert Sequence:

ATGAGTCCAA GATCCCCGCT CAGGATATCT CCATTTGGAG TACACATCCT CATCGCTGCT 
GTGCTCTTTA CGCTAATTCA ATCATCGAAA CAGGACTGCG TTTGGTATGG CGTGTGCAAC 
ACCAATGACT TTTCGCACAG CCAAAACTGT CCGTACAATG GAACAGCGAA GGAAATGGCA 
ACTGACGGTC TGGAGCTGCT GAAGAAACGC TGTGGATTTC TGCTGGAGAA TAGCGAAAAC 
AAATTTTGCT GCGATAAGAA TCAGGTGGAG CTCCTTAATA AGAATGTGGA GCTGGCCGGA 
AATATCTTGG ATCGATGTCC ATCCTGCATG GAGAACTTGG TGCGGCACAT CTGCCAATTC 
ACCTGTTCAC CAAAGCAGGC TGAGTTCATG CATGTGGTGG CCACGCAGAA AAATAAGAAA 
GGTGATGAGT ACATAAGTTC TGTTGACTTG CATATATCAA CGGAATACAT CAACAAGACT 
TATAAATCGT GCTCTCAGGT TTCTGTACCG CAAACGGGTC AATTAGCTTT TGATTTAATG 
TGCGGCGCTT ACTCAGCATC TCGCTGCAAT CCTACAAAAT GGTTCAACTT TATGGGCGAT 
GCCACCAATC CATATGTTCC TTTTCAAATT ACCTATATTC AACATGAGCC CAAATCAAAT 
AGCAATAACT TTACGCCCCT CAATGTAACG ACCGTTCCTT GCAACCAAGC AGTTAGCAGC 
AAACTACCTG CGTGCTCCTG CTCGGACTGC GACCTATCCT GTCCCCAGGG ACCGCCGGAA 
CCGCCCCGTC CAGAGCCCTT CAAAATTGTT GGACTCGACG CCTATTTCGT CATCATGGCA 
GCTGTCTTCT TGGTGGGAGT CTTAGTATTC TTAATGGGCT CGTTTCTGTT TACACAAGGT 
TCGTCCATGG ATGACAACTT TCAAGTGGAT GGCAATGATG TTTCCGATGA AATGCCCTAC 
AGCGAGAATG ATTCATACTT TGAAAAACTT GGCGCCCACA CAGAAACTTT CCTGGAAACA 
TTCTTCACCA AATGGGGAAC GTACTTTGCT AGCAATCCTG GCTTGACTTT AATTGCCGGA 
GCATCCCTGG TTGTTATACT GGGGTATGGC ATCAACTTTA TAGAAATCAC AACGGATCCG 
GTTAAACTTT GGGCTTCGCC GAACTCAAAA TCCAGACTGG AGAGAGAGTT TTTCGATACG 
AAGTTTTCGC CATTCTATAG GCTGGAACAG ATAATCATCA AAGCTGTGAA CTTGCCACAG 
ATTGTGCACA ACACCTCGAA CGGCCCATAC ACCTTCGGTC CCGTCTTCGA CAGAGAATTT 
CTAACAAAAG TGTTGGATCT GCAGGAGGGC ATCAAGGAAA TTAACGCAAA TGGCACACAG 
CTGAAGGATA TATGCTACGC GCCTTTGTCG GATGATGGCA GTGAAATCGA TGTGTCCCAG 
TGCGTTGTCC AATCGATTTG GGGATACTTT GGCGATGATC GAGAAAGATT GGATGACCAC 
GACGAGGACA ATGGATTCAA TGTTACCTAT TTGGATGCCT TATACGATTG CATTAGCAAT 
CCCTATTTAT GCCTAGCTCC TTATGGCGGT CCCGTAGATC CAGCCATAGC ACTTGGAGGA 
TTCCTGCCAC CTGGAGACCA ACTGACTGGC AGCACGAAAT TTGAGTTGGC CAATGCGATC 
ATTCTGACAT TCCTGGTCAA GAATCATCAT AACAAAACCG ATTTGGAAAA TGCCTTAACG 
TGGGAGAAGA AATTCGTGGA GTTTATGACC AACTATACGA AGAACAATAT GTCGCAGTAC 
ATGGACATAG CCTTCACATC GGAGAGATCG ATAGAAGACG AGCTCAACAG GGAGTCCCAG 
TCGGATGTGC TGACTATTTT GGTGTCCTAC CTAATTATGT TCATGTACAT AGCGATTTCG 
CTTGGCCATG TCAAGGAGTT CAAGCGAGTG TTTATCGACA GTAAGATAAC GCTTGGCATA 
GGCGGTGTCA TCATTGTTTT GGCCTCGGTA GTGTCGTCCG TTGGCGTTTT CGGTTACATC 
GGTTTGCCGG CCACCCTGAT CATTGTCGAG GTCATACCGT TTTTAGTATT AGCTGTCGGA 
GTGGATAACA TTTTTATTCT GGTCCAAACT CATCAGCGCG ATCAACGCAA ACCCAATGAA 
ACGCTTGAGC AGCAAGTTGG TCGAATACTC GGCAAGGTTG GACCCAGTAT GCTGCTCACC 
TCGTTGAGTG AAAGCTTTTG CTTCTTTCTC GGTGGCCTTT CTGATATGCC AGCCGTAAGG 
GCTTTTGCCC TATACGCCGG CGTAGCCCTC ATCATTGATT TTCTATTGCA AATAACCTGT 
TTCGTTAGCC TATTTACGTT GGATACGAAG CGCAGGGAGG AGAATCGTAT GGACATTTGT 
TGTTTCATCA AGGGCAAGAA GCCGGATAGT ATAACTAGTA ATGAGGGACT CTTGTACAAA 
TTTTTCAGTA GTGTTTACGT CCCATTTCTG ATGAAGAAAA TTGTGCGCGC CAGTGTGATG 
GTCATCTTTT TTGCCTGGCT GTGCTTCAGC ATTGCCATTG CGCCACGAAT CGACATTGGG 
CTGGATCAAG AGTTGGCTAT GCCGCAGGAT AGTTTCGTCT TGCACTATTT CCAATCGTTG 
AACGAGAATC TCAACATTGG TCCACCAGTG TACTTTGTGC TGAAGGGCGA TCTAGCCTAT 
ACGAATAGTT CGGATCAGAA TTTGGTGTGT GCAGGTCAAT ATTGCAACGA TGACAGCGTA 
CTCACGCAGA TCTACTTAGC CAGTAGGCAC TCTAACCAAA CGTATATCGC GCGTCCAGCA 
TCCAGTTGGA TCGATGACTA CTTCGATTGG GCGGCAGCAG CATCATCCTG TTGCAAATAT 
AGAAAGGATA GCGGCGACTT TTGTCCGCAT CAGGATACTT CCTGTTTGAG GTGTAATATC 
ACGAAGAATT CACTTCTTAG GCCGGAGGAA AAGGAGTTTG TTAAGTATTT GCCATTTTTC 
CTGAAGGACA ATCCCGATGA CACGTGCGCC AAGGCCGGTC ATGCCGCCTA TGGCGGAGCT 
GTTCGGTATT CCAACAGCCA TGAAAGACTG AACATCGAGG CGTCGTATTT TATGGCATAC 
CACACCATAC TTAAGTCCTC GGCCGACTAC TTTCTGGCCC TAGAGTCGGC TAGAAAGATA 
TCCGCGAATA TTACGCAAAT GTTGCAGGGC AGACTAATGT CAAATGGAGT TCCTATGGCA 
TCGGCCCTTA CGGTCGAAGT GTTTCCATAT TCAGTATTCT ATGTGTTTTA CGAACAATAT 
CTGACCATGT GGTCGGATAC GCTACAGAGC ATGGGTATTT CAGTTCTTTC CATATTCGTT 
GTTACATTTG TTTTGATGGG CTTCGATGTT CATTCCGCAT TAGTTGTTGT CATAACGATA 
ACTATGATTG TTGTTAATTT AGGTGGCCTT ATGTATTATT GGAACATTTC GTTGAATGCT 
GTTTCGTTGG TGAATCTCGT CATGGCCGTC GGCATATCCG TGGAATTTTG TTCGCACTTG 
GTGCACAGCT TCGCCACATC GAAATCAGTT AGTCAAATTG ATCGAGCTGC AGATAGCCTT 
TCCAAAATGG GTAGCTCCAT TTTCTCCGGA ATCACTTTAA CCAAATTTGC GGGCATACTA 
GTGTTAGCCT TTGCAAAAAG TCAGATATTC CAGGTGTTTT ATTTCCGTAT GTACTTGGGA 
ATCGTTGTAA TTGGGGCGGC GCACGGGTTG ATCTTTCTGC CCGTTCTATT AAGTTATATT 
GGGGCTCCTG TAAGTAATGC TAGATTAAGG TATCATAGCC AGGCGGCTGC TGAGCACGAG 
ACAGCACTAG CGGGAATACT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npc1a ( NM_001201842.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188771.1 Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant D, mRNA.

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CloneID	OFa14284
Clone ID Related Accession (Same CDS sequence)	NM_001201841.2 , NM_135513.5 , NM_001201840.2 , NM_001201842.2
Accession Version	NM_001201842.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Niemann-Pick type C-1a, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001201842.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCCAA GATCCCCGCT CAGGATATCT CCATTTGGAG TACACATCCT CATCGCTGCT 
GTGCTCTTTA CGCTAATTCA ATCATCGAAA CAGGACTGCG TTTGGTATGG CGTGTGCAAC 
ACCAATGACT TTTCGCACAG CCAAAACTGT CCGTACAATG GAACAGCGAA GGAAATGGCA 
ACTGACGGTC TGGAGCTGCT GAAGAAACGC TGTGGATTTC TGCTGGAGAA TAGCGAAAAC 
AAATTTTGCT GCGATAAGAA TCAGGTGGAG CTCCTTAATA AGAATGTGGA GCTGGCCGGA 
AATATCTTGG ATCGATGTCC ATCCTGCATG GAGAACTTGG TGCGGCACAT CTGCCAATTC 
ACCTGTTCAC CAAAGCAGGC TGAGTTCATG CATGTGGTGG CCACGCAGAA AAATAAGAAA 
GGTGATGAGT ACATAAGTTC TGTTGACTTG CATATATCAA CGGAATACAT CAACAAGACT 
TATAAATCGT GCTCTCAGGT TTCTGTACCG CAAACGGGTC AATTAGCTTT TGATTTAATG 
TGCGGCGCTT ACTCAGCATC TCGCTGCAAT CCTACAAAAT GGTTCAACTT TATGGGCGAT 
GCCACCAATC CATATGTTCC TTTTCAAATT ACCTATATTC AACATGAGCC CAAATCAAAT 
AGCAATAACT TTACGCCCCT CAATGTAACG ACCGTTCCTT GCAACCAAGC AGTTAGCAGC 
AAACTACCTG CGTGCTCCTG CTCGGACTGC GACCTATCCT GTCCCCAGGG ACCGCCGGAA 
CCGCCCCGTC CAGAGCCCTT CAAAATTGTT GGACTCGACG CCTATTTCGT CATCATGGCA 
GCTGTCTTCT TGGTGGGAGT CTTAGTATTC TTAATGGGCT CGTTTCTGTT TACACAAGGT 
TCGTCCATGG ATGACAACTT TCAAGTGGAT GGCAATGATG TTTCCGATGA AATGCCCTAC 
AGCGAGAATG ATTCATACTT TGAAAAACTT GGCGCCCACA CAGAAACTTT CCTGGAAACA 
TTCTTCACCA AATGGGGAAC GTACTTTGCT AGCAATCCTG GCTTGACTTT AATTGCCGGA 
GCATCCCTGG TTGTTATACT GGGGTATGGC ATCAACTTTA TAGAAATCAC AACGGATCCG 
GTTAAACTTT GGGCTTCGCC GAACTCAAAA TCCAGACTGG AGAGAGAGTT TTTCGATACG 
AAGTTTTCGC CATTCTATAG GCTGGAACAG ATAATCATCA AAGCTGTGAA CTTGCCACAG 
ATTGTGCACA ACACCTCGAA CGGCCCATAC ACCTTCGGTC CCGTCTTCGA CAGAGAATTT 
CTAACAAAAG TGTTGGATCT GCAGGAGGGC ATCAAGGAAA TTAACGCAAA TGGCACACAG 
CTGAAGGATA TATGCTACGC GCCTTTGTCG GATGATGGCA GTGAAATCGA TGTGTCCCAG 
TGCGTTGTCC AATCGATTTG GGGATACTTT GGCGATGATC GAGAAAGATT GGATGACCAC 
GACGAGGACA ATGGATTCAA TGTTACCTAT TTGGATGCCT TATACGATTG CATTAGCAAT 
CCCTATTTAT GCCTAGCTCC TTATGGCGGT CCCGTAGATC CAGCCATAGC ACTTGGAGGA 
TTCCTGCCAC CTGGAGACCA ACTGACTGGC AGCACGAAAT TTGAGTTGGC CAATGCGATC 
ATTCTGACAT TCCTGGTCAA GAATCATCAT AACAAAACCG ATTTGGAAAA TGCCTTAACG 
TGGGAGAAGA AATTCGTGGA GTTTATGACC AACTATACGA AGAACAATAT GTCGCAGTAC 
ATGGACATAG CCTTCACATC GGAGAGATCG ATAGAAGACG AGCTCAACAG GGAGTCCCAG 
TCGGATGTGC TGACTATTTT GGTGTCCTAC CTAATTATGT TCATGTACAT AGCGATTTCG 
CTTGGCCATG TCAAGGAGTT CAAGCGAGTG TTTATCGACA GTAAGATAAC GCTTGGCATA 
GGCGGTGTCA TCATTGTTTT GGCCTCGGTA GTGTCGTCCG TTGGCGTTTT CGGTTACATC 
GGTTTGCCGG CCACCCTGAT CATTGTCGAG GTCATACCGT TTTTAGTATT AGCTGTCGGA 
GTGGATAACA TTTTTATTCT GGTCCAAACT CATCAGCGCG ATCAACGCAA ACCCAATGAA 
ACGCTTGAGC AGCAAGTTGG TCGAATACTC GGCAAGGTTG GACCCAGTAT GCTGCTCACC 
TCGTTGAGTG AAAGCTTTTG CTTCTTTCTC GGTGGCCTTT CTGATATGCC AGCCGTAAGG 
GCTTTTGCCC TATACGCCGG CGTAGCCCTC ATCATTGATT TTCTATTGCA AATAACCTGT 
TTCGTTAGCC TATTTACGTT GGATACGAAG CGCAGGGAGG AGAATCGTAT GGACATTTGT 
TGTTTCATCA AGGGCAAGAA GCCGGATAGT ATAACTAGTA ATGAGGGACT CTTGTACAAA 
TTTTTCAGTA GTGTTTACGT CCCATTTCTG ATGAAGAAAA TTGTGCGCGC CAGTGTGATG 
GTCATCTTTT TTGCCTGGCT GTGCTTCAGC ATTGCCATTG CGCCACGAAT CGACATTGGG 
CTGGATCAAG AGTTGGCTAT GCCGCAGGAT AGTTTCGTCT TGCACTATTT CCAATCGTTG 
AACGAGAATC TCAACATTGG TCCACCAGTG TACTTTGTGC TGAAGGGCGA TCTAGCCTAT 
ACGAATAGTT CGGATCAGAA TTTGGTGTGT GCAGGTCAAT ATTGCAACGA TGACAGCGTA 
CTCACGCAGA TCTACTTAGC CAGTAGGCAC TCTAACCAAA CGTATATCGC GCGTCCAGCA 
TCCAGTTGGA TCGATGACTA CTTCGATTGG GCGGCAGCAG CATCATCCTG TTGCAAATAT 
AGAAAGGATA GCGGCGACTT TTGTCCGCAT CAGGATACTT CCTGTTTGAG GTGTAATATC 
ACGAAGAATT CACTTCTTAG GCCGGAGGAA AAGGAGTTTG TTAAGTATTT GCCATTTTTC 
CTGAAGGACA ATCCCGATGA CACGTGCGCC AAGGCCGGTC ATGCCGCCTA TGGCGGAGCT 
GTTCGGTATT CCAACAGCCA TGAAAGACTG AACATCGAGG CGTCGTATTT TATGGCATAC 
CACACCATAC TTAAGTCCTC GGCCGACTAC TTTCTGGCCC TAGAGTCGGC TAGAAAGATA 
TCCGCGAATA TTACGCAAAT GTTGCAGGGC AGACTAATGT CAAATGGAGT TCCTATGGCA 
TCGGCCCTTA CGGTCGAAGT GTTTCCATAT TCAGTATTCT ATGTGTTTTA CGAACAATAT 
CTGACCATGT GGTCGGATAC GCTACAGAGC ATGGGTATTT CAGTTCTTTC CATATTCGTT 
GTTACATTTG TTTTGATGGG CTTCGATGTT CATTCCGCAT TAGTTGTTGT CATAACGATA 
ACTATGATTG TTGTTAATTT AGGTGGCCTT ATGTATTATT GGAACATTTC GTTGAATGCT 
GTTTCGTTGG TGAATCTCGT CATGGCCGTC GGCATATCCG TGGAATTTTG TTCGCACTTG 
GTGCACAGCT TCGCCACATC GAAATCAGTT AGTCAAATTG ATCGAGCTGC AGATAGCCTT 
TCCAAAATGG GTAGCTCCAT TTTCTCCGGA ATCACTTTAA CCAAATTTGC GGGCATACTA 
GTGTTAGCCT TTGCAAAAAG TCAGATATTC CAGGTGTTTT ATTTCCGTAT GTACTTGGGA 
ATCGTTGTAA TTGGGGCGGC GCACGGGTTG ATCTTTCTGC CCGTTCTATT AAGTTATATT 
GGGGCTCCTG TAAGTAATGC TAGATTAAGG TATCATAGCC AGGCGGCTGC TGAGCACGAG 
ACAGCACTAG CGGGAATACT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188771.1
CDS	103..3966
Translation	MSPRSPLRISPFGVHILIAAVLFTLIQSSKQDCVWYGVCNTNDFSHSQNCPYNGTAKEMATDGLELLKKRCGFLLENSENKFCCDKNQVELLNKNVELAGNILDRCPSCMENLVRHICQFTCSPKQAEFMHVVATQKNKKGDEYISSVDLHISTEYINKTYKSCSQVSVPQTGQLAFDLMCGAYSASRCNPTKWFNFMGDATNPYVPFQITYIQHEPKSNSNNFTPLNVTTVPCNQAVSSKLPACSCSDCDLSCPQGPPEPPRPEPFKIVGLDAYFVIMAAVFLVGVLVFLMGSFLFTQGSSMDDNFQVDGNDVSDEMPYSENDSYFEKLGAHTETFLETFFTKWGTYFASNPGLTLIAGASLVVILGYGINFIEITTDPVKLWASPNSKSRLEREFFDTKFSPFYRLEQIIIKAVNLPQIVHNTSNGPYTFGPVFDREFLTKVLDLQEGIKEINANGTQLKDICYAPLSDDGSEIDVSQCVVQSIWGYFGDDRERLDDHDEDNGFNVTYLDALYDCISNPYLCLAPYGGPVDPAIALGGFLPPGDQLTGSTKFELANAIILTFLVKNHHNKTDLENALTWEKKFVEFMTNYTKNNMSQYMDIAFTSERSIEDELNRESQSDVLTILVSYLIMFMYIAISLGHVKEFKRVFIDSKITLGIGGVIIVLASVVSSVGVFGYIGLPATLIIVEVIPFLVLAVGVDNIFILVQTHQRDQRKPNETLEQQVGRILGKVGPSMLLTSLSESFCFFLGGLSDMPAVRAFALYAGVALIIDFLLQITCFVSLFTLDTKRREENRMDICCFIKGKKPDSITSNEGLLYKFFSSVYVPFLMKKIVRASVMVIFFAWLCFSIAIAPRIDIGLDQELAMPQDSFVLHYFQSLNENLNIGPPVYFVLKGDLAYTNSSDQNLVCAGQYCNDDSVLTQIYLASRHSNQTYIARPASSWIDDYFDWAAAASSCCKYRKDSGDFCPHQDTSCLRCNITKNSLLRPEEKEFVKYLPFFLKDNPDDTCAKAGHAAYGGAVRYSNSHERLNIEASYFMAYHTILKSSADYFLALESARKISANITQMLQGRLMSNGVPMASALTVEVFPYSVFYVFYEQYLTMWSDTLQSMGISVLSIFVVTFVLMGFDVHSALVVVITITMIVVNLGGLMYYWNISLNAVSLVNLVMAVGISVEFCSHLVHSFATSKSVSQIDRAADSLSKMGSSIFSGITLTKFAGILVLAFAKSQIFQVFYFRMYLGIVVIGAAHGLIFLPVLLSYIGAPVSNARLRYHSQAAAEHETALAGIL

Target ORF information:

RefSeq Version	NM_001201842.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201842.2

ORF Insert Sequence:

ATGAGTCCAA GATCCCCGCT CAGGATATCT CCATTTGGAG TACACATCCT CATCGCTGCT 
GTGCTCTTTA CGCTAATTCA ATCATCGAAA CAGGACTGCG TTTGGTATGG CGTGTGCAAC 
ACCAATGACT TTTCGCACAG CCAAAACTGT CCGTACAATG GAACAGCGAA GGAAATGGCA 
ACTGACGGTC TGGAGCTGCT GAAGAAACGC TGTGGATTTC TGCTGGAGAA TAGCGAAAAC 
AAATTTTGCT GCGATAAGAA TCAGGTGGAG CTCCTTAATA AGAATGTGGA GCTGGCCGGA 
AATATCTTGG ATCGATGTCC ATCCTGCATG GAGAACTTGG TGCGGCACAT CTGCCAATTC 
ACCTGTTCAC CAAAGCAGGC TGAGTTCATG CATGTGGTGG CCACGCAGAA AAATAAGAAA 
GGTGATGAGT ACATAAGTTC TGTTGACTTG CATATATCAA CGGAATACAT CAACAAGACT 
TATAAATCGT GCTCTCAGGT TTCTGTACCG CAAACGGGTC AATTAGCTTT TGATTTAATG 
TGCGGCGCTT ACTCAGCATC TCGCTGCAAT CCTACAAAAT GGTTCAACTT TATGGGCGAT 
GCCACCAATC CATATGTTCC TTTTCAAATT ACCTATATTC AACATGAGCC CAAATCAAAT 
AGCAATAACT TTACGCCCCT CAATGTAACG ACCGTTCCTT GCAACCAAGC AGTTAGCAGC 
AAACTACCTG CGTGCTCCTG CTCGGACTGC GACCTATCCT GTCCCCAGGG ACCGCCGGAA 
CCGCCCCGTC CAGAGCCCTT CAAAATTGTT GGACTCGACG CCTATTTCGT CATCATGGCA 
GCTGTCTTCT TGGTGGGAGT CTTAGTATTC TTAATGGGCT CGTTTCTGTT TACACAAGGT 
TCGTCCATGG ATGACAACTT TCAAGTGGAT GGCAATGATG TTTCCGATGA AATGCCCTAC 
AGCGAGAATG ATTCATACTT TGAAAAACTT GGCGCCCACA CAGAAACTTT CCTGGAAACA 
TTCTTCACCA AATGGGGAAC GTACTTTGCT AGCAATCCTG GCTTGACTTT AATTGCCGGA 
GCATCCCTGG TTGTTATACT GGGGTATGGC ATCAACTTTA TAGAAATCAC AACGGATCCG 
GTTAAACTTT GGGCTTCGCC GAACTCAAAA TCCAGACTGG AGAGAGAGTT TTTCGATACG 
AAGTTTTCGC CATTCTATAG GCTGGAACAG ATAATCATCA AAGCTGTGAA CTTGCCACAG 
ATTGTGCACA ACACCTCGAA CGGCCCATAC ACCTTCGGTC CCGTCTTCGA CAGAGAATTT 
CTAACAAAAG TGTTGGATCT GCAGGAGGGC ATCAAGGAAA TTAACGCAAA TGGCACACAG 
CTGAAGGATA TATGCTACGC GCCTTTGTCG GATGATGGCA GTGAAATCGA TGTGTCCCAG 
TGCGTTGTCC AATCGATTTG GGGATACTTT GGCGATGATC GAGAAAGATT GGATGACCAC 
GACGAGGACA ATGGATTCAA TGTTACCTAT TTGGATGCCT TATACGATTG CATTAGCAAT 
CCCTATTTAT GCCTAGCTCC TTATGGCGGT CCCGTAGATC CAGCCATAGC ACTTGGAGGA 
TTCCTGCCAC CTGGAGACCA ACTGACTGGC AGCACGAAAT TTGAGTTGGC CAATGCGATC 
ATTCTGACAT TCCTGGTCAA GAATCATCAT AACAAAACCG ATTTGGAAAA TGCCTTAACG 
TGGGAGAAGA AATTCGTGGA GTTTATGACC AACTATACGA AGAACAATAT GTCGCAGTAC 
ATGGACATAG CCTTCACATC GGAGAGATCG ATAGAAGACG AGCTCAACAG GGAGTCCCAG 
TCGGATGTGC TGACTATTTT GGTGTCCTAC CTAATTATGT TCATGTACAT AGCGATTTCG 
CTTGGCCATG TCAAGGAGTT CAAGCGAGTG TTTATCGACA GTAAGATAAC GCTTGGCATA 
GGCGGTGTCA TCATTGTTTT GGCCTCGGTA GTGTCGTCCG TTGGCGTTTT CGGTTACATC 
GGTTTGCCGG CCACCCTGAT CATTGTCGAG GTCATACCGT TTTTAGTATT AGCTGTCGGA 
GTGGATAACA TTTTTATTCT GGTCCAAACT CATCAGCGCG ATCAACGCAA ACCCAATGAA 
ACGCTTGAGC AGCAAGTTGG TCGAATACTC GGCAAGGTTG GACCCAGTAT GCTGCTCACC 
TCGTTGAGTG AAAGCTTTTG CTTCTTTCTC GGTGGCCTTT CTGATATGCC AGCCGTAAGG 
GCTTTTGCCC TATACGCCGG CGTAGCCCTC ATCATTGATT TTCTATTGCA AATAACCTGT 
TTCGTTAGCC TATTTACGTT GGATACGAAG CGCAGGGAGG AGAATCGTAT GGACATTTGT 
TGTTTCATCA AGGGCAAGAA GCCGGATAGT ATAACTAGTA ATGAGGGACT CTTGTACAAA 
TTTTTCAGTA GTGTTTACGT CCCATTTCTG ATGAAGAAAA TTGTGCGCGC CAGTGTGATG 
GTCATCTTTT TTGCCTGGCT GTGCTTCAGC ATTGCCATTG CGCCACGAAT CGACATTGGG 
CTGGATCAAG AGTTGGCTAT GCCGCAGGAT AGTTTCGTCT TGCACTATTT CCAATCGTTG 
AACGAGAATC TCAACATTGG TCCACCAGTG TACTTTGTGC TGAAGGGCGA TCTAGCCTAT 
ACGAATAGTT CGGATCAGAA TTTGGTGTGT GCAGGTCAAT ATTGCAACGA TGACAGCGTA 
CTCACGCAGA TCTACTTAGC CAGTAGGCAC TCTAACCAAA CGTATATCGC GCGTCCAGCA 
TCCAGTTGGA TCGATGACTA CTTCGATTGG GCGGCAGCAG CATCATCCTG TTGCAAATAT 
AGAAAGGATA GCGGCGACTT TTGTCCGCAT CAGGATACTT CCTGTTTGAG GTGTAATATC 
ACGAAGAATT CACTTCTTAG GCCGGAGGAA AAGGAGTTTG TTAAGTATTT GCCATTTTTC 
CTGAAGGACA ATCCCGATGA CACGTGCGCC AAGGCCGGTC ATGCCGCCTA TGGCGGAGCT 
GTTCGGTATT CCAACAGCCA TGAAAGACTG AACATCGAGG CGTCGTATTT TATGGCATAC 
CACACCATAC TTAAGTCCTC GGCCGACTAC TTTCTGGCCC TAGAGTCGGC TAGAAAGATA 
TCCGCGAATA TTACGCAAAT GTTGCAGGGC AGACTAATGT CAAATGGAGT TCCTATGGCA 
TCGGCCCTTA CGGTCGAAGT GTTTCCATAT TCAGTATTCT ATGTGTTTTA CGAACAATAT 
CTGACCATGT GGTCGGATAC GCTACAGAGC ATGGGTATTT CAGTTCTTTC CATATTCGTT 
GTTACATTTG TTTTGATGGG CTTCGATGTT CATTCCGCAT TAGTTGTTGT CATAACGATA 
ACTATGATTG TTGTTAATTT AGGTGGCCTT ATGTATTATT GGAACATTTC GTTGAATGCT 
GTTTCGTTGG TGAATCTCGT CATGGCCGTC GGCATATCCG TGGAATTTTG TTCGCACTTG 
GTGCACAGCT TCGCCACATC GAAATCAGTT AGTCAAATTG ATCGAGCTGC AGATAGCCTT 
TCCAAAATGG GTAGCTCCAT TTTCTCCGGA ATCACTTTAA CCAAATTTGC GGGCATACTA 
GTGTTAGCCT TTGCAAAAAG TCAGATATTC CAGGTGTTTT ATTTCCGTAT GTACTTGGGA 
ATCGTTGTAA TTGGGGCGGC GCACGGGTTG ATCTTTCTGC CCGTTCTATT AAGTTATATT 
GGGGCTCCTG TAAGTAATGC TAGATTAAGG TATCATAGCC AGGCGGCTGC TGAGCACGAG 
ACAGCACTAG CGGGAATACT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npc1a ( NM_001298867.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285796.1 Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa21622
Clone ID Related Accession (Same CDS sequence)	NM_001298867.1 , NM_001298866.1 , NM_001298868.1
Accession Version	NM_001298867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Niemann-Pick type C-1a, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCCAA GATCCCCGCT CAGGATATCT CCATTTGGAG TACACATCCT CATCGCTGCT 
GTGCTCTTTA CGCTAATTCA ATCATCGAAA CAGGACTGCG TTTGGTATGG CGTGTGCAAC 
ACCAATGACT TTTCGCACAG CCAAAACTGT CCGTACAATG GAACAGCGAA GGAAATGGCA 
ACTGACGGTC TGGAGCTGCT GAAGAAACGC TGTGGATTTC TGCTGGAGAA TAGCGAAAAC 
AAATTTTGCT GCGATAAGAA TCAGGTGGAG CTCCTTAATA AGAATGTGGA GCTGGCCGGA 
AATATCTTGG ATCGATGTCC ATCCTGCATG GAGAACTTGG TGCGGCACAT CTGCCAATTC 
ACCTGTTCAC CAAAGCAGGC TGAGTTCATG CATGTGGTGG CCACGCAGAA AAATAAGAAA 
GGTGATGAGT ACATAAGTTC TGTTGACTTG CATATATCAA CGGAATACAT CAACAAGACT 
TATAAATCGT GCTCTCAGGT TTCTGTACCG CAAACGGGTC AATTAGCTTT TGATTTAATG 
TGCGGCGCTT ACTCAGCATC TCGCTGCAAT CCTACAAAAT GGTTCAACTT TATGGGCGAT 
GCCACCAATC CATATGTTCC TTTTCAAATT ACCTATATTC AACATGAGCC CAAATCAAAT 
AGCAATAACT TTACGCCCCT CAATGTAACG ACCGTTCCTT GCAACCAAGC AGTTAGCAGC 
AAACTACCTG CGTGCTCCTG CTCGGACTGC GACCTATCCT GTCCCCAGGG ACCGCCGGAA 
CCGCCCCGTC CAGAGCCCTT CAAAATTGTT GGACTCGACG CCTATTTCGT CATCATGGCA 
GCTGTCTTCT TGGTGGGAGT CTTAGTATTC TTAATGGGCT CGTTTCTGTT TACACAAGAT 
GACAACTTTC AAGTGGATGG CAATGATGTT TCCGATGAAA TGCCCTACAG CGAGAATGAT 
TCATACTTTG AAAAACTTGG CGCCCACACA GAAACTTTCC TGGAAACATT CTTCACCAAA 
TGGGGAACGT ACTTTGCTAG CAATCCTGGC TTGACTTTAA TTGCCGGAGC ATCCCTGGTT 
GTTATACTGG GGTATGGCAT CAACTTTATA GAAATCACAA CGGATCCGGT TAAACTTTGG 
GCTTCGCCGA ACTCAAAATC CAGACTGGAG AGAGAGTTTT TCGATACGAA GTTTTCGCCA 
TTCTATAGGC TGGAACAGAT AATCATCAAA GCTGTGAACT TGCCACAGAT TGTGCACAAC 
ACCTCGAACG GCCCATACAC CTTCGGTCCC GTCTTCGACA GAGAATTTCT AACAAAAGTG 
TTGGATCTGC AGGAGGGCAT CAAGGAAATT AACGCAAATG GCACACAGCT GAAGGATATA 
TGCTACGCGC CTTTGTCGGA TGATGGCAGT GAAATCGATG TGTCCCAGTG CGTTGTCCAA 
TCGATTTGGG GATACTTTGG CGATGATCGA GAAAGATTGG ATGACCACGA CGAGGACAAT 
GGATTCAATG TTACCTATTT GGATGCCTTA TACGATTGCA TTAGCAATCC CTATTTATGC 
CTAGCTCCTT ATGGCGGTCC CGTAGATCCA GCCATAGCAC TTGGAGGATT CCTGCCACCT 
GGAGACCAAC TGACTGGCAG CACGAAATTT GAGTTGGCCA ATGCGATCAT TCTGACATTC 
CTGGTCAAGA ATCATCATAA CAAAACCGAT TTGGAAAATG CCTTAACGTG GGAGAAGAAA 
TTCGTGGAGT TTATGACCAA CTATACGAAG AACAATATGT CGCAGTACAT GGACATAGCC 
TTCACATCGG AGAGATCGAT AGAAGACGAG CTCAACAGGG AGTCCCAGTC GGATGTGCTG 
ACTATTTTGG TGTCCTACCT AATTATGTTC ATGTACATAG CGATTTCGCT TGGCCATGTC 
AAGGAGTTCA AGCGAGTGTT TATCGACAGT AAGATAACGC TTGGCATAGG CGGTGTCATC 
ATTGTTTTGG CCTCGGTAGT GTCGTCCGTT GGCGTTTTCG GTTACATCGG TTTGCCGGCC 
ACCCTGATCA TTGTCGAGGT CATACCGTTT TTAGTATTAG CTGTCGGAGT GGATAACATT 
TTTATTCTGG TCCAAACTCA TCAGCGCGAT CAACGCAAAC CCAATGAAAC GCTTGAGCAG 
CAAGTTGGTC GAATACTCGG CAAGGTTGGA CCCAGTATGC TGCTCACCTC GTTGAGTGAA 
AGCTTTTGCT TCTTTCTCGG TGGCCTTTCT GATATGCCAG CCGTAAGGGC TTTTGCCCTA 
TACGCCGGCG TAGCCCTCAT CATTGATTTT CTATTGCAAA TAACCTGTTT CGTTAGCCTA 
TTTACGTTGG ATACGAAGCG CAGGGAGGAG AATCGTATGG ACATTTGTTG TTTCATCAAG 
GGCAAGAAGC CGGATAGTAT AACTAGTAAT GAGGGACTCT TGTACAAATT TTTCAGTAGT 
GTTTACGTCC CATTTCTGAT GAAGAAAATT GTGCGCGCCA GTGTGATGGT CATCTTTTTT 
GCCTGGCTGT GCTTCAGCAT TGCCATTGCG CCACGAATCG ACATTGGGCT GGATCAAGAG 
TTGGCTATGC CGCAGGATAG TTTCGTCTTG CACTATTTCC AATCGTTGAA CGAGAATCTC 
AACATTGGTC CACCAGTGTA CTTTGTGCTG AAGGGCGATC TAGCCTATAC GAATAGTTCG 
GATCAGAATT TGGTGTGTGC AGGTCAATAT TGCAACGATG ACAGCGTACT CACGCAGATC 
TACTTAGCCA GTAGGCACTC TAACCAAACG TATATCGCGC GTCCAGCATC CAGTTGGATC 
GATGACTACT TCGATTGGGC GGCAGCAGCA TCATCCTGTT GCAAATATAG AAAGGATAGC 
GGCGACTTTT GTCCGCATCA GGATACTTCC TGTTTGAGGT GTAATATCAC GAAGAATTCA 
CTTCTTAGGC CGGAGGAAAA GGAGTTTGTT AAGTATTTGC CATTTTTCCT GAAGGACAAT 
CCCGATGACA CGTGCGCCAA GGCCGGTCAT GCCGCCTATG GCGGAGCTGT TCGGTATTCC 
AACAGCCATG AAAGACTGAA CATCGAGGCG TCGTATTTTA TGGCATACCA CACCATACTT 
AAGTCCTCGG CCGACTACTT TCTGGCCCTA GAGTCGGCTA GAAAGATATC CGCGAATATT 
ACGCAAATGT TGCAGGGCAG ACTAATGTCA AATGGAGTTC CTATGGCATC GGCCCTTACG 
GTCGAAGTGT TTCCATATTC AGTATTCTAT GTGTTTTACG AACAATATCT GACCATGTGG 
TCGGATACGC TACAGAGCAT GGGTATTTCA GTTCTTTCCA TATTCGTTGT TACATTTGTT 
TTGATGGGCT TCGATGTTCA TTCCGCATTA GTTGTTGTCA TAACGATAAC TATGATTGTT 
GTTAATTTAG GTGGCCTTAT GTATTATTGG AACATTTCGT TGAATGCTGT TTCGTTGGTG 
AATCTCGTCA TGGCCGTCGG CATATCCGTG GAATTTTGTT CGCACTTGGT GCACAGCTTC 
GCCACATCGA AATCAGTTAG TCAAATTGAT CGAGCTGCAG ATAGCCTTTC CAAAATGGGT 
AGCTCCATTT TCTCCGGAAT CACTTTAACC AAATTTGCGG GCATACTAGT GTTAGCCTTT 
GCAAAAAGTC AGATATTCCA GGTGTTTTAT TTCCGTATGT ACTTGGGAAT CGTTGTAATT 
GGGGCGGCGC ACGGGTTGAT CTTTCTGCCC GTTCTATTAA GTTATATTGG GGCTCCTGTA 
AGTAATGCTA GATTAAGGTA TCATAGCCAG GCGGCTGCTG AGCACGAGAC AGCACTAGCG 
GGAATACTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285796.1
CDS	100..3951
Translation	MSPRSPLRISPFGVHILIAAVLFTLIQSSKQDCVWYGVCNTNDFSHSQNCPYNGTAKEMATDGLELLKKRCGFLLENSENKFCCDKNQVELLNKNVELAGNILDRCPSCMENLVRHICQFTCSPKQAEFMHVVATQKNKKGDEYISSVDLHISTEYINKTYKSCSQVSVPQTGQLAFDLMCGAYSASRCNPTKWFNFMGDATNPYVPFQITYIQHEPKSNSNNFTPLNVTTVPCNQAVSSKLPACSCSDCDLSCPQGPPEPPRPEPFKIVGLDAYFVIMAAVFLVGVLVFLMGSFLFTQDDNFQVDGNDVSDEMPYSENDSYFEKLGAHTETFLETFFTKWGTYFASNPGLTLIAGASLVVILGYGINFIEITTDPVKLWASPNSKSRLEREFFDTKFSPFYRLEQIIIKAVNLPQIVHNTSNGPYTFGPVFDREFLTKVLDLQEGIKEINANGTQLKDICYAPLSDDGSEIDVSQCVVQSIWGYFGDDRERLDDHDEDNGFNVTYLDALYDCISNPYLCLAPYGGPVDPAIALGGFLPPGDQLTGSTKFELANAIILTFLVKNHHNKTDLENALTWEKKFVEFMTNYTKNNMSQYMDIAFTSERSIEDELNRESQSDVLTILVSYLIMFMYIAISLGHVKEFKRVFIDSKITLGIGGVIIVLASVVSSVGVFGYIGLPATLIIVEVIPFLVLAVGVDNIFILVQTHQRDQRKPNETLEQQVGRILGKVGPSMLLTSLSESFCFFLGGLSDMPAVRAFALYAGVALIIDFLLQITCFVSLFTLDTKRREENRMDICCFIKGKKPDSITSNEGLLYKFFSSVYVPFLMKKIVRASVMVIFFAWLCFSIAIAPRIDIGLDQELAMPQDSFVLHYFQSLNENLNIGPPVYFVLKGDLAYTNSSDQNLVCAGQYCNDDSVLTQIYLASRHSNQTYIARPASSWIDDYFDWAAAASSCCKYRKDSGDFCPHQDTSCLRCNITKNSLLRPEEKEFVKYLPFFLKDNPDDTCAKAGHAAYGGAVRYSNSHERLNIEASYFMAYHTILKSSADYFLALESARKISANITQMLQGRLMSNGVPMASALTVEVFPYSVFYVFYEQYLTMWSDTLQSMGISVLSIFVVTFVLMGFDVHSALVVVITITMIVVNLGGLMYYWNISLNAVSLVNLVMAVGISVEFCSHLVHSFATSKSVSQIDRAADSLSKMGSSIFSGITLTKFAGILVLAFAKSQIFQVFYFRMYLGIVVIGAAHGLIFLPVLLSYIGAPVSNARLRYHSQAAAEHETALAGIL

Target ORF information:

RefSeq Version	NM_001298867.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298867.1

ORF Insert Sequence:

ATGAGTCCAA GATCCCCGCT CAGGATATCT CCATTTGGAG TACACATCCT CATCGCTGCT 
GTGCTCTTTA CGCTAATTCA ATCATCGAAA CAGGACTGCG TTTGGTATGG CGTGTGCAAC 
ACCAATGACT TTTCGCACAG CCAAAACTGT CCGTACAATG GAACAGCGAA GGAAATGGCA 
ACTGACGGTC TGGAGCTGCT GAAGAAACGC TGTGGATTTC TGCTGGAGAA TAGCGAAAAC 
AAATTTTGCT GCGATAAGAA TCAGGTGGAG CTCCTTAATA AGAATGTGGA GCTGGCCGGA 
AATATCTTGG ATCGATGTCC ATCCTGCATG GAGAACTTGG TGCGGCACAT CTGCCAATTC 
ACCTGTTCAC CAAAGCAGGC TGAGTTCATG CATGTGGTGG CCACGCAGAA AAATAAGAAA 
GGTGATGAGT ACATAAGTTC TGTTGACTTG CATATATCAA CGGAATACAT CAACAAGACT 
TATAAATCGT GCTCTCAGGT TTCTGTACCG CAAACGGGTC AATTAGCTTT TGATTTAATG 
TGCGGCGCTT ACTCAGCATC TCGCTGCAAT CCTACAAAAT GGTTCAACTT TATGGGCGAT 
GCCACCAATC CATATGTTCC TTTTCAAATT ACCTATATTC AACATGAGCC CAAATCAAAT 
AGCAATAACT TTACGCCCCT CAATGTAACG ACCGTTCCTT GCAACCAAGC AGTTAGCAGC 
AAACTACCTG CGTGCTCCTG CTCGGACTGC GACCTATCCT GTCCCCAGGG ACCGCCGGAA 
CCGCCCCGTC CAGAGCCCTT CAAAATTGTT GGACTCGACG CCTATTTCGT CATCATGGCA 
GCTGTCTTCT TGGTGGGAGT CTTAGTATTC TTAATGGGCT CGTTTCTGTT TACACAAGAT 
GACAACTTTC AAGTGGATGG CAATGATGTT TCCGATGAAA TGCCCTACAG CGAGAATGAT 
TCATACTTTG AAAAACTTGG CGCCCACACA GAAACTTTCC TGGAAACATT CTTCACCAAA 
TGGGGAACGT ACTTTGCTAG CAATCCTGGC TTGACTTTAA TTGCCGGAGC ATCCCTGGTT 
GTTATACTGG GGTATGGCAT CAACTTTATA GAAATCACAA CGGATCCGGT TAAACTTTGG 
GCTTCGCCGA ACTCAAAATC CAGACTGGAG AGAGAGTTTT TCGATACGAA GTTTTCGCCA 
TTCTATAGGC TGGAACAGAT AATCATCAAA GCTGTGAACT TGCCACAGAT TGTGCACAAC 
ACCTCGAACG GCCCATACAC CTTCGGTCCC GTCTTCGACA GAGAATTTCT AACAAAAGTG 
TTGGATCTGC AGGAGGGCAT CAAGGAAATT AACGCAAATG GCACACAGCT GAAGGATATA 
TGCTACGCGC CTTTGTCGGA TGATGGCAGT GAAATCGATG TGTCCCAGTG CGTTGTCCAA 
TCGATTTGGG GATACTTTGG CGATGATCGA GAAAGATTGG ATGACCACGA CGAGGACAAT 
GGATTCAATG TTACCTATTT GGATGCCTTA TACGATTGCA TTAGCAATCC CTATTTATGC 
CTAGCTCCTT ATGGCGGTCC CGTAGATCCA GCCATAGCAC TTGGAGGATT CCTGCCACCT 
GGAGACCAAC TGACTGGCAG CACGAAATTT GAGTTGGCCA ATGCGATCAT TCTGACATTC 
CTGGTCAAGA ATCATCATAA CAAAACCGAT TTGGAAAATG CCTTAACGTG GGAGAAGAAA 
TTCGTGGAGT TTATGACCAA CTATACGAAG AACAATATGT CGCAGTACAT GGACATAGCC 
TTCACATCGG AGAGATCGAT AGAAGACGAG CTCAACAGGG AGTCCCAGTC GGATGTGCTG 
ACTATTTTGG TGTCCTACCT AATTATGTTC ATGTACATAG CGATTTCGCT TGGCCATGTC 
AAGGAGTTCA AGCGAGTGTT TATCGACAGT AAGATAACGC TTGGCATAGG CGGTGTCATC 
ATTGTTTTGG CCTCGGTAGT GTCGTCCGTT GGCGTTTTCG GTTACATCGG TTTGCCGGCC 
ACCCTGATCA TTGTCGAGGT CATACCGTTT TTAGTATTAG CTGTCGGAGT GGATAACATT 
TTTATTCTGG TCCAAACTCA TCAGCGCGAT CAACGCAAAC CCAATGAAAC GCTTGAGCAG 
CAAGTTGGTC GAATACTCGG CAAGGTTGGA CCCAGTATGC TGCTCACCTC GTTGAGTGAA 
AGCTTTTGCT TCTTTCTCGG TGGCCTTTCT GATATGCCAG CCGTAAGGGC TTTTGCCCTA 
TACGCCGGCG TAGCCCTCAT CATTGATTTT CTATTGCAAA TAACCTGTTT CGTTAGCCTA 
TTTACGTTGG ATACGAAGCG CAGGGAGGAG AATCGTATGG ACATTTGTTG TTTCATCAAG 
GGCAAGAAGC CGGATAGTAT AACTAGTAAT GAGGGACTCT TGTACAAATT TTTCAGTAGT 
GTTTACGTCC CATTTCTGAT GAAGAAAATT GTGCGCGCCA GTGTGATGGT CATCTTTTTT 
GCCTGGCTGT GCTTCAGCAT TGCCATTGCG CCACGAATCG ACATTGGGCT GGATCAAGAG 
TTGGCTATGC CGCAGGATAG TTTCGTCTTG CACTATTTCC AATCGTTGAA CGAGAATCTC 
AACATTGGTC CACCAGTGTA CTTTGTGCTG AAGGGCGATC TAGCCTATAC GAATAGTTCG 
GATCAGAATT TGGTGTGTGC AGGTCAATAT TGCAACGATG ACAGCGTACT CACGCAGATC 
TACTTAGCCA GTAGGCACTC TAACCAAACG TATATCGCGC GTCCAGCATC CAGTTGGATC 
GATGACTACT TCGATTGGGC GGCAGCAGCA TCATCCTGTT GCAAATATAG AAAGGATAGC 
GGCGACTTTT GTCCGCATCA GGATACTTCC TGTTTGAGGT GTAATATCAC GAAGAATTCA 
CTTCTTAGGC CGGAGGAAAA GGAGTTTGTT AAGTATTTGC CATTTTTCCT GAAGGACAAT 
CCCGATGACA CGTGCGCCAA GGCCGGTCAT GCCGCCTATG GCGGAGCTGT TCGGTATTCC 
AACAGCCATG AAAGACTGAA CATCGAGGCG TCGTATTTTA TGGCATACCA CACCATACTT 
AAGTCCTCGG CCGACTACTT TCTGGCCCTA GAGTCGGCTA GAAAGATATC CGCGAATATT 
ACGCAAATGT TGCAGGGCAG ACTAATGTCA AATGGAGTTC CTATGGCATC GGCCCTTACG 
GTCGAAGTGT TTCCATATTC AGTATTCTAT GTGTTTTACG AACAATATCT GACCATGTGG 
TCGGATACGC TACAGAGCAT GGGTATTTCA GTTCTTTCCA TATTCGTTGT TACATTTGTT 
TTGATGGGCT TCGATGTTCA TTCCGCATTA GTTGTTGTCA TAACGATAAC TATGATTGTT 
GTTAATTTAG GTGGCCTTAT GTATTATTGG AACATTTCGT TGAATGCTGT TTCGTTGGTG 
AATCTCGTCA TGGCCGTCGG CATATCCGTG GAATTTTGTT CGCACTTGGT GCACAGCTTC 
GCCACATCGA AATCAGTTAG TCAAATTGAT CGAGCTGCAG ATAGCCTTTC CAAAATGGGT 
AGCTCCATTT TCTCCGGAAT CACTTTAACC AAATTTGCGG GCATACTAGT GTTAGCCTTT 
GCAAAAAGTC AGATATTCCA GGTGTTTTAT TTCCGTATGT ACTTGGGAAT CGTTGTAATT 
GGGGCGGCGC ACGGGTTGAT CTTTCTGCCC GTTCTATTAA GTTATATTGG GGCTCCTGTA 
AGTAATGCTA GATTAAGGTA TCATAGCCAG GCGGCTGCTG AGCACGAGAC AGCACTAGCG 
GGAATACTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npc1a ( NM_001298866.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285795.1 Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa21622
Clone ID Related Accession (Same CDS sequence)	NM_001298867.1 , NM_001298866.1 , NM_001298868.1
Accession Version	NM_001298866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Niemann-Pick type C-1a, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCCAA GATCCCCGCT CAGGATATCT CCATTTGGAG TACACATCCT CATCGCTGCT 
GTGCTCTTTA CGCTAATTCA ATCATCGAAA CAGGACTGCG TTTGGTATGG CGTGTGCAAC 
ACCAATGACT TTTCGCACAG CCAAAACTGT CCGTACAATG GAACAGCGAA GGAAATGGCA 
ACTGACGGTC TGGAGCTGCT GAAGAAACGC TGTGGATTTC TGCTGGAGAA TAGCGAAAAC 
AAATTTTGCT GCGATAAGAA TCAGGTGGAG CTCCTTAATA AGAATGTGGA GCTGGCCGGA 
AATATCTTGG ATCGATGTCC ATCCTGCATG GAGAACTTGG TGCGGCACAT CTGCCAATTC 
ACCTGTTCAC CAAAGCAGGC TGAGTTCATG CATGTGGTGG CCACGCAGAA AAATAAGAAA 
GGTGATGAGT ACATAAGTTC TGTTGACTTG CATATATCAA CGGAATACAT CAACAAGACT 
TATAAATCGT GCTCTCAGGT TTCTGTACCG CAAACGGGTC AATTAGCTTT TGATTTAATG 
TGCGGCGCTT ACTCAGCATC TCGCTGCAAT CCTACAAAAT GGTTCAACTT TATGGGCGAT 
GCCACCAATC CATATGTTCC TTTTCAAATT ACCTATATTC AACATGAGCC CAAATCAAAT 
AGCAATAACT TTACGCCCCT CAATGTAACG ACCGTTCCTT GCAACCAAGC AGTTAGCAGC 
AAACTACCTG CGTGCTCCTG CTCGGACTGC GACCTATCCT GTCCCCAGGG ACCGCCGGAA 
CCGCCCCGTC CAGAGCCCTT CAAAATTGTT GGACTCGACG CCTATTTCGT CATCATGGCA 
GCTGTCTTCT TGGTGGGAGT CTTAGTATTC TTAATGGGCT CGTTTCTGTT TACACAAGAT 
GACAACTTTC AAGTGGATGG CAATGATGTT TCCGATGAAA TGCCCTACAG CGAGAATGAT 
TCATACTTTG AAAAACTTGG CGCCCACACA GAAACTTTCC TGGAAACATT CTTCACCAAA 
TGGGGAACGT ACTTTGCTAG CAATCCTGGC TTGACTTTAA TTGCCGGAGC ATCCCTGGTT 
GTTATACTGG GGTATGGCAT CAACTTTATA GAAATCACAA CGGATCCGGT TAAACTTTGG 
GCTTCGCCGA ACTCAAAATC CAGACTGGAG AGAGAGTTTT TCGATACGAA GTTTTCGCCA 
TTCTATAGGC TGGAACAGAT AATCATCAAA GCTGTGAACT TGCCACAGAT TGTGCACAAC 
ACCTCGAACG GCCCATACAC CTTCGGTCCC GTCTTCGACA GAGAATTTCT AACAAAAGTG 
TTGGATCTGC AGGAGGGCAT CAAGGAAATT AACGCAAATG GCACACAGCT GAAGGATATA 
TGCTACGCGC CTTTGTCGGA TGATGGCAGT GAAATCGATG TGTCCCAGTG CGTTGTCCAA 
TCGATTTGGG GATACTTTGG CGATGATCGA GAAAGATTGG ATGACCACGA CGAGGACAAT 
GGATTCAATG TTACCTATTT GGATGCCTTA TACGATTGCA TTAGCAATCC CTATTTATGC 
CTAGCTCCTT ATGGCGGTCC CGTAGATCCA GCCATAGCAC TTGGAGGATT CCTGCCACCT 
GGAGACCAAC TGACTGGCAG CACGAAATTT GAGTTGGCCA ATGCGATCAT TCTGACATTC 
CTGGTCAAGA ATCATCATAA CAAAACCGAT TTGGAAAATG CCTTAACGTG GGAGAAGAAA 
TTCGTGGAGT TTATGACCAA CTATACGAAG AACAATATGT CGCAGTACAT GGACATAGCC 
TTCACATCGG AGAGATCGAT AGAAGACGAG CTCAACAGGG AGTCCCAGTC GGATGTGCTG 
ACTATTTTGG TGTCCTACCT AATTATGTTC ATGTACATAG CGATTTCGCT TGGCCATGTC 
AAGGAGTTCA AGCGAGTGTT TATCGACAGT AAGATAACGC TTGGCATAGG CGGTGTCATC 
ATTGTTTTGG CCTCGGTAGT GTCGTCCGTT GGCGTTTTCG GTTACATCGG TTTGCCGGCC 
ACCCTGATCA TTGTCGAGGT CATACCGTTT TTAGTATTAG CTGTCGGAGT GGATAACATT 
TTTATTCTGG TCCAAACTCA TCAGCGCGAT CAACGCAAAC CCAATGAAAC GCTTGAGCAG 
CAAGTTGGTC GAATACTCGG CAAGGTTGGA CCCAGTATGC TGCTCACCTC GTTGAGTGAA 
AGCTTTTGCT TCTTTCTCGG TGGCCTTTCT GATATGCCAG CCGTAAGGGC TTTTGCCCTA 
TACGCCGGCG TAGCCCTCAT CATTGATTTT CTATTGCAAA TAACCTGTTT CGTTAGCCTA 
TTTACGTTGG ATACGAAGCG CAGGGAGGAG AATCGTATGG ACATTTGTTG TTTCATCAAG 
GGCAAGAAGC CGGATAGTAT AACTAGTAAT GAGGGACTCT TGTACAAATT TTTCAGTAGT 
GTTTACGTCC CATTTCTGAT GAAGAAAATT GTGCGCGCCA GTGTGATGGT CATCTTTTTT 
GCCTGGCTGT GCTTCAGCAT TGCCATTGCG CCACGAATCG ACATTGGGCT GGATCAAGAG 
TTGGCTATGC CGCAGGATAG TTTCGTCTTG CACTATTTCC AATCGTTGAA CGAGAATCTC 
AACATTGGTC CACCAGTGTA CTTTGTGCTG AAGGGCGATC TAGCCTATAC GAATAGTTCG 
GATCAGAATT TGGTGTGTGC AGGTCAATAT TGCAACGATG ACAGCGTACT CACGCAGATC 
TACTTAGCCA GTAGGCACTC TAACCAAACG TATATCGCGC GTCCAGCATC CAGTTGGATC 
GATGACTACT TCGATTGGGC GGCAGCAGCA TCATCCTGTT GCAAATATAG AAAGGATAGC 
GGCGACTTTT GTCCGCATCA GGATACTTCC TGTTTGAGGT GTAATATCAC GAAGAATTCA 
CTTCTTAGGC CGGAGGAAAA GGAGTTTGTT AAGTATTTGC CATTTTTCCT GAAGGACAAT 
CCCGATGACA CGTGCGCCAA GGCCGGTCAT GCCGCCTATG GCGGAGCTGT TCGGTATTCC 
AACAGCCATG AAAGACTGAA CATCGAGGCG TCGTATTTTA TGGCATACCA CACCATACTT 
AAGTCCTCGG CCGACTACTT TCTGGCCCTA GAGTCGGCTA GAAAGATATC CGCGAATATT 
ACGCAAATGT TGCAGGGCAG ACTAATGTCA AATGGAGTTC CTATGGCATC GGCCCTTACG 
GTCGAAGTGT TTCCATATTC AGTATTCTAT GTGTTTTACG AACAATATCT GACCATGTGG 
TCGGATACGC TACAGAGCAT GGGTATTTCA GTTCTTTCCA TATTCGTTGT TACATTTGTT 
TTGATGGGCT TCGATGTTCA TTCCGCATTA GTTGTTGTCA TAACGATAAC TATGATTGTT 
GTTAATTTAG GTGGCCTTAT GTATTATTGG AACATTTCGT TGAATGCTGT TTCGTTGGTG 
AATCTCGTCA TGGCCGTCGG CATATCCGTG GAATTTTGTT CGCACTTGGT GCACAGCTTC 
GCCACATCGA AATCAGTTAG TCAAATTGAT CGAGCTGCAG ATAGCCTTTC CAAAATGGGT 
AGCTCCATTT TCTCCGGAAT CACTTTAACC AAATTTGCGG GCATACTAGT GTTAGCCTTT 
GCAAAAAGTC AGATATTCCA GGTGTTTTAT TTCCGTATGT ACTTGGGAAT CGTTGTAATT 
GGGGCGGCGC ACGGGTTGAT CTTTCTGCCC GTTCTATTAA GTTATATTGG GGCTCCTGTA 
AGTAATGCTA GATTAAGGTA TCATAGCCAG GCGGCTGCTG AGCACGAGAC AGCACTAGCG 
GGAATACTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285795.1
CDS	279..4130
Translation	MSPRSPLRISPFGVHILIAAVLFTLIQSSKQDCVWYGVCNTNDFSHSQNCPYNGTAKEMATDGLELLKKRCGFLLENSENKFCCDKNQVELLNKNVELAGNILDRCPSCMENLVRHICQFTCSPKQAEFMHVVATQKNKKGDEYISSVDLHISTEYINKTYKSCSQVSVPQTGQLAFDLMCGAYSASRCNPTKWFNFMGDATNPYVPFQITYIQHEPKSNSNNFTPLNVTTVPCNQAVSSKLPACSCSDCDLSCPQGPPEPPRPEPFKIVGLDAYFVIMAAVFLVGVLVFLMGSFLFTQDDNFQVDGNDVSDEMPYSENDSYFEKLGAHTETFLETFFTKWGTYFASNPGLTLIAGASLVVILGYGINFIEITTDPVKLWASPNSKSRLEREFFDTKFSPFYRLEQIIIKAVNLPQIVHNTSNGPYTFGPVFDREFLTKVLDLQEGIKEINANGTQLKDICYAPLSDDGSEIDVSQCVVQSIWGYFGDDRERLDDHDEDNGFNVTYLDALYDCISNPYLCLAPYGGPVDPAIALGGFLPPGDQLTGSTKFELANAIILTFLVKNHHNKTDLENALTWEKKFVEFMTNYTKNNMSQYMDIAFTSERSIEDELNRESQSDVLTILVSYLIMFMYIAISLGHVKEFKRVFIDSKITLGIGGVIIVLASVVSSVGVFGYIGLPATLIIVEVIPFLVLAVGVDNIFILVQTHQRDQRKPNETLEQQVGRILGKVGPSMLLTSLSESFCFFLGGLSDMPAVRAFALYAGVALIIDFLLQITCFVSLFTLDTKRREENRMDICCFIKGKKPDSITSNEGLLYKFFSSVYVPFLMKKIVRASVMVIFFAWLCFSIAIAPRIDIGLDQELAMPQDSFVLHYFQSLNENLNIGPPVYFVLKGDLAYTNSSDQNLVCAGQYCNDDSVLTQIYLASRHSNQTYIARPASSWIDDYFDWAAAASSCCKYRKDSGDFCPHQDTSCLRCNITKNSLLRPEEKEFVKYLPFFLKDNPDDTCAKAGHAAYGGAVRYSNSHERLNIEASYFMAYHTILKSSADYFLALESARKISANITQMLQGRLMSNGVPMASALTVEVFPYSVFYVFYEQYLTMWSDTLQSMGISVLSIFVVTFVLMGFDVHSALVVVITITMIVVNLGGLMYYWNISLNAVSLVNLVMAVGISVEFCSHLVHSFATSKSVSQIDRAADSLSKMGSSIFSGITLTKFAGILVLAFAKSQIFQVFYFRMYLGIVVIGAAHGLIFLPVLLSYIGAPVSNARLRYHSQAAAEHETALAGIL

Target ORF information:

RefSeq Version	NM_001298866.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298866.1

ORF Insert Sequence:

ATGAGTCCAA GATCCCCGCT CAGGATATCT CCATTTGGAG TACACATCCT CATCGCTGCT 
GTGCTCTTTA CGCTAATTCA ATCATCGAAA CAGGACTGCG TTTGGTATGG CGTGTGCAAC 
ACCAATGACT TTTCGCACAG CCAAAACTGT CCGTACAATG GAACAGCGAA GGAAATGGCA 
ACTGACGGTC TGGAGCTGCT GAAGAAACGC TGTGGATTTC TGCTGGAGAA TAGCGAAAAC 
AAATTTTGCT GCGATAAGAA TCAGGTGGAG CTCCTTAATA AGAATGTGGA GCTGGCCGGA 
AATATCTTGG ATCGATGTCC ATCCTGCATG GAGAACTTGG TGCGGCACAT CTGCCAATTC 
ACCTGTTCAC CAAAGCAGGC TGAGTTCATG CATGTGGTGG CCACGCAGAA AAATAAGAAA 
GGTGATGAGT ACATAAGTTC TGTTGACTTG CATATATCAA CGGAATACAT CAACAAGACT 
TATAAATCGT GCTCTCAGGT TTCTGTACCG CAAACGGGTC AATTAGCTTT TGATTTAATG 
TGCGGCGCTT ACTCAGCATC TCGCTGCAAT CCTACAAAAT GGTTCAACTT TATGGGCGAT 
GCCACCAATC CATATGTTCC TTTTCAAATT ACCTATATTC AACATGAGCC CAAATCAAAT 
AGCAATAACT TTACGCCCCT CAATGTAACG ACCGTTCCTT GCAACCAAGC AGTTAGCAGC 
AAACTACCTG CGTGCTCCTG CTCGGACTGC GACCTATCCT GTCCCCAGGG ACCGCCGGAA 
CCGCCCCGTC CAGAGCCCTT CAAAATTGTT GGACTCGACG CCTATTTCGT CATCATGGCA 
GCTGTCTTCT TGGTGGGAGT CTTAGTATTC TTAATGGGCT CGTTTCTGTT TACACAAGAT 
GACAACTTTC AAGTGGATGG CAATGATGTT TCCGATGAAA TGCCCTACAG CGAGAATGAT 
TCATACTTTG AAAAACTTGG CGCCCACACA GAAACTTTCC TGGAAACATT CTTCACCAAA 
TGGGGAACGT ACTTTGCTAG CAATCCTGGC TTGACTTTAA TTGCCGGAGC ATCCCTGGTT 
GTTATACTGG GGTATGGCAT CAACTTTATA GAAATCACAA CGGATCCGGT TAAACTTTGG 
GCTTCGCCGA ACTCAAAATC CAGACTGGAG AGAGAGTTTT TCGATACGAA GTTTTCGCCA 
TTCTATAGGC TGGAACAGAT AATCATCAAA GCTGTGAACT TGCCACAGAT TGTGCACAAC 
ACCTCGAACG GCCCATACAC CTTCGGTCCC GTCTTCGACA GAGAATTTCT AACAAAAGTG 
TTGGATCTGC AGGAGGGCAT CAAGGAAATT AACGCAAATG GCACACAGCT GAAGGATATA 
TGCTACGCGC CTTTGTCGGA TGATGGCAGT GAAATCGATG TGTCCCAGTG CGTTGTCCAA 
TCGATTTGGG GATACTTTGG CGATGATCGA GAAAGATTGG ATGACCACGA CGAGGACAAT 
GGATTCAATG TTACCTATTT GGATGCCTTA TACGATTGCA TTAGCAATCC CTATTTATGC 
CTAGCTCCTT ATGGCGGTCC CGTAGATCCA GCCATAGCAC TTGGAGGATT CCTGCCACCT 
GGAGACCAAC TGACTGGCAG CACGAAATTT GAGTTGGCCA ATGCGATCAT TCTGACATTC 
CTGGTCAAGA ATCATCATAA CAAAACCGAT TTGGAAAATG CCTTAACGTG GGAGAAGAAA 
TTCGTGGAGT TTATGACCAA CTATACGAAG AACAATATGT CGCAGTACAT GGACATAGCC 
TTCACATCGG AGAGATCGAT AGAAGACGAG CTCAACAGGG AGTCCCAGTC GGATGTGCTG 
ACTATTTTGG TGTCCTACCT AATTATGTTC ATGTACATAG CGATTTCGCT TGGCCATGTC 
AAGGAGTTCA AGCGAGTGTT TATCGACAGT AAGATAACGC TTGGCATAGG CGGTGTCATC 
ATTGTTTTGG CCTCGGTAGT GTCGTCCGTT GGCGTTTTCG GTTACATCGG TTTGCCGGCC 
ACCCTGATCA TTGTCGAGGT CATACCGTTT TTAGTATTAG CTGTCGGAGT GGATAACATT 
TTTATTCTGG TCCAAACTCA TCAGCGCGAT CAACGCAAAC CCAATGAAAC GCTTGAGCAG 
CAAGTTGGTC GAATACTCGG CAAGGTTGGA CCCAGTATGC TGCTCACCTC GTTGAGTGAA 
AGCTTTTGCT TCTTTCTCGG TGGCCTTTCT GATATGCCAG CCGTAAGGGC TTTTGCCCTA 
TACGCCGGCG TAGCCCTCAT CATTGATTTT CTATTGCAAA TAACCTGTTT CGTTAGCCTA 
TTTACGTTGG ATACGAAGCG CAGGGAGGAG AATCGTATGG ACATTTGTTG TTTCATCAAG 
GGCAAGAAGC CGGATAGTAT AACTAGTAAT GAGGGACTCT TGTACAAATT TTTCAGTAGT 
GTTTACGTCC CATTTCTGAT GAAGAAAATT GTGCGCGCCA GTGTGATGGT CATCTTTTTT 
GCCTGGCTGT GCTTCAGCAT TGCCATTGCG CCACGAATCG ACATTGGGCT GGATCAAGAG 
TTGGCTATGC CGCAGGATAG TTTCGTCTTG CACTATTTCC AATCGTTGAA CGAGAATCTC 
AACATTGGTC CACCAGTGTA CTTTGTGCTG AAGGGCGATC TAGCCTATAC GAATAGTTCG 
GATCAGAATT TGGTGTGTGC AGGTCAATAT TGCAACGATG ACAGCGTACT CACGCAGATC 
TACTTAGCCA GTAGGCACTC TAACCAAACG TATATCGCGC GTCCAGCATC CAGTTGGATC 
GATGACTACT TCGATTGGGC GGCAGCAGCA TCATCCTGTT GCAAATATAG AAAGGATAGC 
GGCGACTTTT GTCCGCATCA GGATACTTCC TGTTTGAGGT GTAATATCAC GAAGAATTCA 
CTTCTTAGGC CGGAGGAAAA GGAGTTTGTT AAGTATTTGC CATTTTTCCT GAAGGACAAT 
CCCGATGACA CGTGCGCCAA GGCCGGTCAT GCCGCCTATG GCGGAGCTGT TCGGTATTCC 
AACAGCCATG AAAGACTGAA CATCGAGGCG TCGTATTTTA TGGCATACCA CACCATACTT 
AAGTCCTCGG CCGACTACTT TCTGGCCCTA GAGTCGGCTA GAAAGATATC CGCGAATATT 
ACGCAAATGT TGCAGGGCAG ACTAATGTCA AATGGAGTTC CTATGGCATC GGCCCTTACG 
GTCGAAGTGT TTCCATATTC AGTATTCTAT GTGTTTTACG AACAATATCT GACCATGTGG 
TCGGATACGC TACAGAGCAT GGGTATTTCA GTTCTTTCCA TATTCGTTGT TACATTTGTT 
TTGATGGGCT TCGATGTTCA TTCCGCATTA GTTGTTGTCA TAACGATAAC TATGATTGTT 
GTTAATTTAG GTGGCCTTAT GTATTATTGG AACATTTCGT TGAATGCTGT TTCGTTGGTG 
AATCTCGTCA TGGCCGTCGG CATATCCGTG GAATTTTGTT CGCACTTGGT GCACAGCTTC 
GCCACATCGA AATCAGTTAG TCAAATTGAT CGAGCTGCAG ATAGCCTTTC CAAAATGGGT 
AGCTCCATTT TCTCCGGAAT CACTTTAACC AAATTTGCGG GCATACTAGT GTTAGCCTTT 
GCAAAAAGTC AGATATTCCA GGTGTTTTAT TTCCGTATGT ACTTGGGAAT CGTTGTAATT 
GGGGCGGCGC ACGGGTTGAT CTTTCTGCCC GTTCTATTAA GTTATATTGG GGCTCCTGTA 
AGTAATGCTA GATTAAGGTA TCATAGCCAG GCGGCTGCTG AGCACGAGAC AGCACTAGCG 
GGAATACTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npc1a ( NM_001298868.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285797.1 Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant G, mRNA.

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CloneID	OFa21622
Clone ID Related Accession (Same CDS sequence)	NM_001298867.1 , NM_001298866.1 , NM_001298868.1
Accession Version	NM_001298868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Niemann-Pick type C-1a, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCCAA GATCCCCGCT CAGGATATCT CCATTTGGAG TACACATCCT CATCGCTGCT 
GTGCTCTTTA CGCTAATTCA ATCATCGAAA CAGGACTGCG TTTGGTATGG CGTGTGCAAC 
ACCAATGACT TTTCGCACAG CCAAAACTGT CCGTACAATG GAACAGCGAA GGAAATGGCA 
ACTGACGGTC TGGAGCTGCT GAAGAAACGC TGTGGATTTC TGCTGGAGAA TAGCGAAAAC 
AAATTTTGCT GCGATAAGAA TCAGGTGGAG CTCCTTAATA AGAATGTGGA GCTGGCCGGA 
AATATCTTGG ATCGATGTCC ATCCTGCATG GAGAACTTGG TGCGGCACAT CTGCCAATTC 
ACCTGTTCAC CAAAGCAGGC TGAGTTCATG CATGTGGTGG CCACGCAGAA AAATAAGAAA 
GGTGATGAGT ACATAAGTTC TGTTGACTTG CATATATCAA CGGAATACAT CAACAAGACT 
TATAAATCGT GCTCTCAGGT TTCTGTACCG CAAACGGGTC AATTAGCTTT TGATTTAATG 
TGCGGCGCTT ACTCAGCATC TCGCTGCAAT CCTACAAAAT GGTTCAACTT TATGGGCGAT 
GCCACCAATC CATATGTTCC TTTTCAAATT ACCTATATTC AACATGAGCC CAAATCAAAT 
AGCAATAACT TTACGCCCCT CAATGTAACG ACCGTTCCTT GCAACCAAGC AGTTAGCAGC 
AAACTACCTG CGTGCTCCTG CTCGGACTGC GACCTATCCT GTCCCCAGGG ACCGCCGGAA 
CCGCCCCGTC CAGAGCCCTT CAAAATTGTT GGACTCGACG CCTATTTCGT CATCATGGCA 
GCTGTCTTCT TGGTGGGAGT CTTAGTATTC TTAATGGGCT CGTTTCTGTT TACACAAGAT 
GACAACTTTC AAGTGGATGG CAATGATGTT TCCGATGAAA TGCCCTACAG CGAGAATGAT 
TCATACTTTG AAAAACTTGG CGCCCACACA GAAACTTTCC TGGAAACATT CTTCACCAAA 
TGGGGAACGT ACTTTGCTAG CAATCCTGGC TTGACTTTAA TTGCCGGAGC ATCCCTGGTT 
GTTATACTGG GGTATGGCAT CAACTTTATA GAAATCACAA CGGATCCGGT TAAACTTTGG 
GCTTCGCCGA ACTCAAAATC CAGACTGGAG AGAGAGTTTT TCGATACGAA GTTTTCGCCA 
TTCTATAGGC TGGAACAGAT AATCATCAAA GCTGTGAACT TGCCACAGAT TGTGCACAAC 
ACCTCGAACG GCCCATACAC CTTCGGTCCC GTCTTCGACA GAGAATTTCT AACAAAAGTG 
TTGGATCTGC AGGAGGGCAT CAAGGAAATT AACGCAAATG GCACACAGCT GAAGGATATA 
TGCTACGCGC CTTTGTCGGA TGATGGCAGT GAAATCGATG TGTCCCAGTG CGTTGTCCAA 
TCGATTTGGG GATACTTTGG CGATGATCGA GAAAGATTGG ATGACCACGA CGAGGACAAT 
GGATTCAATG TTACCTATTT GGATGCCTTA TACGATTGCA TTAGCAATCC CTATTTATGC 
CTAGCTCCTT ATGGCGGTCC CGTAGATCCA GCCATAGCAC TTGGAGGATT CCTGCCACCT 
GGAGACCAAC TGACTGGCAG CACGAAATTT GAGTTGGCCA ATGCGATCAT TCTGACATTC 
CTGGTCAAGA ATCATCATAA CAAAACCGAT TTGGAAAATG CCTTAACGTG GGAGAAGAAA 
TTCGTGGAGT TTATGACCAA CTATACGAAG AACAATATGT CGCAGTACAT GGACATAGCC 
TTCACATCGG AGAGATCGAT AGAAGACGAG CTCAACAGGG AGTCCCAGTC GGATGTGCTG 
ACTATTTTGG TGTCCTACCT AATTATGTTC ATGTACATAG CGATTTCGCT TGGCCATGTC 
AAGGAGTTCA AGCGAGTGTT TATCGACAGT AAGATAACGC TTGGCATAGG CGGTGTCATC 
ATTGTTTTGG CCTCGGTAGT GTCGTCCGTT GGCGTTTTCG GTTACATCGG TTTGCCGGCC 
ACCCTGATCA TTGTCGAGGT CATACCGTTT TTAGTATTAG CTGTCGGAGT GGATAACATT 
TTTATTCTGG TCCAAACTCA TCAGCGCGAT CAACGCAAAC CCAATGAAAC GCTTGAGCAG 
CAAGTTGGTC GAATACTCGG CAAGGTTGGA CCCAGTATGC TGCTCACCTC GTTGAGTGAA 
AGCTTTTGCT TCTTTCTCGG TGGCCTTTCT GATATGCCAG CCGTAAGGGC TTTTGCCCTA 
TACGCCGGCG TAGCCCTCAT CATTGATTTT CTATTGCAAA TAACCTGTTT CGTTAGCCTA 
TTTACGTTGG ATACGAAGCG CAGGGAGGAG AATCGTATGG ACATTTGTTG TTTCATCAAG 
GGCAAGAAGC CGGATAGTAT AACTAGTAAT GAGGGACTCT TGTACAAATT TTTCAGTAGT 
GTTTACGTCC CATTTCTGAT GAAGAAAATT GTGCGCGCCA GTGTGATGGT CATCTTTTTT 
GCCTGGCTGT GCTTCAGCAT TGCCATTGCG CCACGAATCG ACATTGGGCT GGATCAAGAG 
TTGGCTATGC CGCAGGATAG TTTCGTCTTG CACTATTTCC AATCGTTGAA CGAGAATCTC 
AACATTGGTC CACCAGTGTA CTTTGTGCTG AAGGGCGATC TAGCCTATAC GAATAGTTCG 
GATCAGAATT TGGTGTGTGC AGGTCAATAT TGCAACGATG ACAGCGTACT CACGCAGATC 
TACTTAGCCA GTAGGCACTC TAACCAAACG TATATCGCGC GTCCAGCATC CAGTTGGATC 
GATGACTACT TCGATTGGGC GGCAGCAGCA TCATCCTGTT GCAAATATAG AAAGGATAGC 
GGCGACTTTT GTCCGCATCA GGATACTTCC TGTTTGAGGT GTAATATCAC GAAGAATTCA 
CTTCTTAGGC CGGAGGAAAA GGAGTTTGTT AAGTATTTGC CATTTTTCCT GAAGGACAAT 
CCCGATGACA CGTGCGCCAA GGCCGGTCAT GCCGCCTATG GCGGAGCTGT TCGGTATTCC 
AACAGCCATG AAAGACTGAA CATCGAGGCG TCGTATTTTA TGGCATACCA CACCATACTT 
AAGTCCTCGG CCGACTACTT TCTGGCCCTA GAGTCGGCTA GAAAGATATC CGCGAATATT 
ACGCAAATGT TGCAGGGCAG ACTAATGTCA AATGGAGTTC CTATGGCATC GGCCCTTACG 
GTCGAAGTGT TTCCATATTC AGTATTCTAT GTGTTTTACG AACAATATCT GACCATGTGG 
TCGGATACGC TACAGAGCAT GGGTATTTCA GTTCTTTCCA TATTCGTTGT TACATTTGTT 
TTGATGGGCT TCGATGTTCA TTCCGCATTA GTTGTTGTCA TAACGATAAC TATGATTGTT 
GTTAATTTAG GTGGCCTTAT GTATTATTGG AACATTTCGT TGAATGCTGT TTCGTTGGTG 
AATCTCGTCA TGGCCGTCGG CATATCCGTG GAATTTTGTT CGCACTTGGT GCACAGCTTC 
GCCACATCGA AATCAGTTAG TCAAATTGAT CGAGCTGCAG ATAGCCTTTC CAAAATGGGT 
AGCTCCATTT TCTCCGGAAT CACTTTAACC AAATTTGCGG GCATACTAGT GTTAGCCTTT 
GCAAAAAGTC AGATATTCCA GGTGTTTTAT TTCCGTATGT ACTTGGGAAT CGTTGTAATT 
GGGGCGGCGC ACGGGTTGAT CTTTCTGCCC GTTCTATTAA GTTATATTGG GGCTCCTGTA 
AGTAATGCTA GATTAAGGTA TCATAGCCAG GCGGCTGCTG AGCACGAGAC AGCACTAGCG 
GGAATACTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285797.1
CDS	1093..4944
Translation	MSPRSPLRISPFGVHILIAAVLFTLIQSSKQDCVWYGVCNTNDFSHSQNCPYNGTAKEMATDGLELLKKRCGFLLENSENKFCCDKNQVELLNKNVELAGNILDRCPSCMENLVRHICQFTCSPKQAEFMHVVATQKNKKGDEYISSVDLHISTEYINKTYKSCSQVSVPQTGQLAFDLMCGAYSASRCNPTKWFNFMGDATNPYVPFQITYIQHEPKSNSNNFTPLNVTTVPCNQAVSSKLPACSCSDCDLSCPQGPPEPPRPEPFKIVGLDAYFVIMAAVFLVGVLVFLMGSFLFTQDDNFQVDGNDVSDEMPYSENDSYFEKLGAHTETFLETFFTKWGTYFASNPGLTLIAGASLVVILGYGINFIEITTDPVKLWASPNSKSRLEREFFDTKFSPFYRLEQIIIKAVNLPQIVHNTSNGPYTFGPVFDREFLTKVLDLQEGIKEINANGTQLKDICYAPLSDDGSEIDVSQCVVQSIWGYFGDDRERLDDHDEDNGFNVTYLDALYDCISNPYLCLAPYGGPVDPAIALGGFLPPGDQLTGSTKFELANAIILTFLVKNHHNKTDLENALTWEKKFVEFMTNYTKNNMSQYMDIAFTSERSIEDELNRESQSDVLTILVSYLIMFMYIAISLGHVKEFKRVFIDSKITLGIGGVIIVLASVVSSVGVFGYIGLPATLIIVEVIPFLVLAVGVDNIFILVQTHQRDQRKPNETLEQQVGRILGKVGPSMLLTSLSESFCFFLGGLSDMPAVRAFALYAGVALIIDFLLQITCFVSLFTLDTKRREENRMDICCFIKGKKPDSITSNEGLLYKFFSSVYVPFLMKKIVRASVMVIFFAWLCFSIAIAPRIDIGLDQELAMPQDSFVLHYFQSLNENLNIGPPVYFVLKGDLAYTNSSDQNLVCAGQYCNDDSVLTQIYLASRHSNQTYIARPASSWIDDYFDWAAAASSCCKYRKDSGDFCPHQDTSCLRCNITKNSLLRPEEKEFVKYLPFFLKDNPDDTCAKAGHAAYGGAVRYSNSHERLNIEASYFMAYHTILKSSADYFLALESARKISANITQMLQGRLMSNGVPMASALTVEVFPYSVFYVFYEQYLTMWSDTLQSMGISVLSIFVVTFVLMGFDVHSALVVVITITMIVVNLGGLMYYWNISLNAVSLVNLVMAVGISVEFCSHLVHSFATSKSVSQIDRAADSLSKMGSSIFSGITLTKFAGILVLAFAKSQIFQVFYFRMYLGIVVIGAAHGLIFLPVLLSYIGAPVSNARLRYHSQAAAEHETALAGIL

Target ORF information:

RefSeq Version	NM_001298868.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Niemann-Pick type C-1a (Npc1a), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298868.1

ORF Insert Sequence:

ATGAGTCCAA GATCCCCGCT CAGGATATCT CCATTTGGAG TACACATCCT CATCGCTGCT 
GTGCTCTTTA CGCTAATTCA ATCATCGAAA CAGGACTGCG TTTGGTATGG CGTGTGCAAC 
ACCAATGACT TTTCGCACAG CCAAAACTGT CCGTACAATG GAACAGCGAA GGAAATGGCA 
ACTGACGGTC TGGAGCTGCT GAAGAAACGC TGTGGATTTC TGCTGGAGAA TAGCGAAAAC 
AAATTTTGCT GCGATAAGAA TCAGGTGGAG CTCCTTAATA AGAATGTGGA GCTGGCCGGA 
AATATCTTGG ATCGATGTCC ATCCTGCATG GAGAACTTGG TGCGGCACAT CTGCCAATTC 
ACCTGTTCAC CAAAGCAGGC TGAGTTCATG CATGTGGTGG CCACGCAGAA AAATAAGAAA 
GGTGATGAGT ACATAAGTTC TGTTGACTTG CATATATCAA CGGAATACAT CAACAAGACT 
TATAAATCGT GCTCTCAGGT TTCTGTACCG CAAACGGGTC AATTAGCTTT TGATTTAATG 
TGCGGCGCTT ACTCAGCATC TCGCTGCAAT CCTACAAAAT GGTTCAACTT TATGGGCGAT 
GCCACCAATC CATATGTTCC TTTTCAAATT ACCTATATTC AACATGAGCC CAAATCAAAT 
AGCAATAACT TTACGCCCCT CAATGTAACG ACCGTTCCTT GCAACCAAGC AGTTAGCAGC 
AAACTACCTG CGTGCTCCTG CTCGGACTGC GACCTATCCT GTCCCCAGGG ACCGCCGGAA 
CCGCCCCGTC CAGAGCCCTT CAAAATTGTT GGACTCGACG CCTATTTCGT CATCATGGCA 
GCTGTCTTCT TGGTGGGAGT CTTAGTATTC TTAATGGGCT CGTTTCTGTT TACACAAGAT 
GACAACTTTC AAGTGGATGG CAATGATGTT TCCGATGAAA TGCCCTACAG CGAGAATGAT 
TCATACTTTG AAAAACTTGG CGCCCACACA GAAACTTTCC TGGAAACATT CTTCACCAAA 
TGGGGAACGT ACTTTGCTAG CAATCCTGGC TTGACTTTAA TTGCCGGAGC ATCCCTGGTT 
GTTATACTGG GGTATGGCAT CAACTTTATA GAAATCACAA CGGATCCGGT TAAACTTTGG 
GCTTCGCCGA ACTCAAAATC CAGACTGGAG AGAGAGTTTT TCGATACGAA GTTTTCGCCA 
TTCTATAGGC TGGAACAGAT AATCATCAAA GCTGTGAACT TGCCACAGAT TGTGCACAAC 
ACCTCGAACG GCCCATACAC CTTCGGTCCC GTCTTCGACA GAGAATTTCT AACAAAAGTG 
TTGGATCTGC AGGAGGGCAT CAAGGAAATT AACGCAAATG GCACACAGCT GAAGGATATA 
TGCTACGCGC CTTTGTCGGA TGATGGCAGT GAAATCGATG TGTCCCAGTG CGTTGTCCAA 
TCGATTTGGG GATACTTTGG CGATGATCGA GAAAGATTGG ATGACCACGA CGAGGACAAT 
GGATTCAATG TTACCTATTT GGATGCCTTA TACGATTGCA TTAGCAATCC CTATTTATGC 
CTAGCTCCTT ATGGCGGTCC CGTAGATCCA GCCATAGCAC TTGGAGGATT CCTGCCACCT 
GGAGACCAAC TGACTGGCAG CACGAAATTT GAGTTGGCCA ATGCGATCAT TCTGACATTC 
CTGGTCAAGA ATCATCATAA CAAAACCGAT TTGGAAAATG CCTTAACGTG GGAGAAGAAA 
TTCGTGGAGT TTATGACCAA CTATACGAAG AACAATATGT CGCAGTACAT GGACATAGCC 
TTCACATCGG AGAGATCGAT AGAAGACGAG CTCAACAGGG AGTCCCAGTC GGATGTGCTG 
ACTATTTTGG TGTCCTACCT AATTATGTTC ATGTACATAG CGATTTCGCT TGGCCATGTC 
AAGGAGTTCA AGCGAGTGTT TATCGACAGT AAGATAACGC TTGGCATAGG CGGTGTCATC 
ATTGTTTTGG CCTCGGTAGT GTCGTCCGTT GGCGTTTTCG GTTACATCGG TTTGCCGGCC 
ACCCTGATCA TTGTCGAGGT CATACCGTTT TTAGTATTAG CTGTCGGAGT GGATAACATT 
TTTATTCTGG TCCAAACTCA TCAGCGCGAT CAACGCAAAC CCAATGAAAC GCTTGAGCAG 
CAAGTTGGTC GAATACTCGG CAAGGTTGGA CCCAGTATGC TGCTCACCTC GTTGAGTGAA 
AGCTTTTGCT TCTTTCTCGG TGGCCTTTCT GATATGCCAG CCGTAAGGGC TTTTGCCCTA 
TACGCCGGCG TAGCCCTCAT CATTGATTTT CTATTGCAAA TAACCTGTTT CGTTAGCCTA 
TTTACGTTGG ATACGAAGCG CAGGGAGGAG AATCGTATGG ACATTTGTTG TTTCATCAAG 
GGCAAGAAGC CGGATAGTAT AACTAGTAAT GAGGGACTCT TGTACAAATT TTTCAGTAGT 
GTTTACGTCC CATTTCTGAT GAAGAAAATT GTGCGCGCCA GTGTGATGGT CATCTTTTTT 
GCCTGGCTGT GCTTCAGCAT TGCCATTGCG CCACGAATCG ACATTGGGCT GGATCAAGAG 
TTGGCTATGC CGCAGGATAG TTTCGTCTTG CACTATTTCC AATCGTTGAA CGAGAATCTC 
AACATTGGTC CACCAGTGTA CTTTGTGCTG AAGGGCGATC TAGCCTATAC GAATAGTTCG 
GATCAGAATT TGGTGTGTGC AGGTCAATAT TGCAACGATG ACAGCGTACT CACGCAGATC 
TACTTAGCCA GTAGGCACTC TAACCAAACG TATATCGCGC GTCCAGCATC CAGTTGGATC 
GATGACTACT TCGATTGGGC GGCAGCAGCA TCATCCTGTT GCAAATATAG AAAGGATAGC 
GGCGACTTTT GTCCGCATCA GGATACTTCC TGTTTGAGGT GTAATATCAC GAAGAATTCA 
CTTCTTAGGC CGGAGGAAAA GGAGTTTGTT AAGTATTTGC CATTTTTCCT GAAGGACAAT 
CCCGATGACA CGTGCGCCAA GGCCGGTCAT GCCGCCTATG GCGGAGCTGT TCGGTATTCC 
AACAGCCATG AAAGACTGAA CATCGAGGCG TCGTATTTTA TGGCATACCA CACCATACTT 
AAGTCCTCGG CCGACTACTT TCTGGCCCTA GAGTCGGCTA GAAAGATATC CGCGAATATT 
ACGCAAATGT TGCAGGGCAG ACTAATGTCA AATGGAGTTC CTATGGCATC GGCCCTTACG 
GTCGAAGTGT TTCCATATTC AGTATTCTAT GTGTTTTACG AACAATATCT GACCATGTGG 
TCGGATACGC TACAGAGCAT GGGTATTTCA GTTCTTTCCA TATTCGTTGT TACATTTGTT 
TTGATGGGCT TCGATGTTCA TTCCGCATTA GTTGTTGTCA TAACGATAAC TATGATTGTT 
GTTAATTTAG GTGGCCTTAT GTATTATTGG AACATTTCGT TGAATGCTGT TTCGTTGGTG 
AATCTCGTCA TGGCCGTCGG CATATCCGTG GAATTTTGTT CGCACTTGGT GCACAGCTTC 
GCCACATCGA AATCAGTTAG TCAAATTGAT CGAGCTGCAG ATAGCCTTTC CAAAATGGGT 
AGCTCCATTT TCTCCGGAAT CACTTTAACC AAATTTGCGG GCATACTAGT GTTAGCCTTT 
GCAAAAAGTC AGATATTCCA GGTGTTTTAT TTCCGTATGT ACTTGGGAAT CGTTGTAATT 
GGGGCGGCGC ACGGGTTGAT CTTTCTGCCC GTTCTATTAA GTTATATTGG GGCTCCTGTA 
AGTAATGCTA GATTAAGGTA TCATAGCCAG GCGGCTGCTG AGCACGAGAC AGCACTAGCG 
GGAATACTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Npc1a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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