bib cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bib
Entrez Gene ID	34330
Full Name	big brain
Synonyms	BIB_DROME,Bib,CG4722,Dmel\CG4722,EP(2)2278,l(2)neo66,l(2)neo91,m-bib
General protein information	Preferred Names big brain Names CG4722 gene product from transcript CG4722-RA Big brain CG4722-PA CG4722-PB bib-PA bib-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 30F5-30F5\|2-34.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273384.2	NP_001260313.1	big brain, isoform B
NM_057489.5	NP_476837.1	big brain, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bib-transfected L cells formed aggregated clusters, while control-L cells remained as a single cell suspension. Ion permeation was not confirmed in L cells stably expressing Bib.
Title: Drosophila big brain does not act as a water channel, but mediates cell adhesion.

Big brain, a Drosophila homologue of mammalian aquaporin, is regulated by the DRE/DREF system
Title: Big brain, a Drosophila homologue of mammalian aquaporin, is regulated by the DRE/DREF system.

The dominant-negative construct of BIB could be a tool for testing a role for BIB ion channels during nervous system development in Drosophila.
Title: Dominant-negative suppression of big brain ion channel activity by mutation of a conserved glutamate in the first transmembrane domain.

The following bib gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bib cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14262	NM_057489.5 Latest version!	Drosophila melanogaster big brain (bib), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa15968	NM_001273384.2 Latest version!	Drosophila melanogaster big brain (bib), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bib ( NM_057489.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476837.1 Drosophila melanogaster big brain (bib), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14262
Clone ID Related Accession (Same CDS sequence)	NM_057489.5
Accession Version	NM_057489.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	big brain, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057489.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGACG AAAGTCTGCA CACCGTGCCC CTGGAGCACA ACATAGACTA CCACATCGTG 
ACGCTATTCG AGCGCCTGGA GGCGATGCGT AAGGACTCGC ACGGCGGTGG CCACGGGGTC 
AACAATCGCC TGTCCAGCAC CCTGCAGGCT CCCAAGCGGA GCATGCAGGC TGAGATTCGC 
ACGCTGGAGT TTTGGAGATC CATCATCAGC GAGTGTCTGG CCTCCTTCAT GTACGTGTTC 
ATCGTCTGCG GTGCCGCCGC TGGCGTCGGA GTGGGGGCCA GTGTGTCGTC CGTGCTTTTG 
GCCACGGCTC TGGCCTCCGG CCTGGCGATG GCTACACTGA CGCAGTGCTT CCTCCACATC 
TCGGGCGCCC ACATCAATCC CGCCGTAACC CTGGCTCTAT GCGTAGTGCG ATCCATATCT 
CCGATCCGGG CTGCCATGTA CATAACCGCA CAATGTGGCG GAGGAATCGC CGGAGCTGCT 
CTGCTTTATG GCGTAACTGT GCCTGGTTAC CAGGGGAATC TGCAGGCCGC CATCTCACAT 
AGCGCTGCTC TGGCCGCCTG GGAAAGATTC GGTGTGGAAT TCATCCTCAC CTTTCTGGTG 
GTTCTGTGCT ATTTCGTATC CACGGATCCC ATGAAGAAGT TCATGGGCAA TTCGGCCGCC 
TCGATTGGAT GTGCTTACAG CGCCTGCTGC TTTGTGTCGA TGCCGTACCT GAATCCAGCC 
CGCTCCCTGG GTCCTTCGTT TGTGCTTAAC AAATGGGACA GCCATTGGGT GTACTGGTTC 
GGACCACTGG TGGGCGGCAT GGCCTCTGGC CTGGTGTACG AGTACATCTT CAACTCGCGC 
AACCGCAACC TGCGCCACAA CAAGGGCAGC ATCGACAACG ATTCCAGTTC GATTCACTCG 
GAGGACGAAC TGAACTACGA CATGGATATG GAGAAGCCCA ACAAGTATCA GCAGTCGCAG 
GGCACCTACC CGCGTGGCCA GTCCAATGGC AATGGCGGAG GACAGGCGGC CGGAAATGGT 
CAGCACCAAG CTGCCAACAT GGGCCAGATG CCGGGCGTAG TGGCCAATGC CGGTCAGGGC 
AATTACTGCC AGAATCTGTA CACTGCTCCG CCGCTCTCCT CGAAGTACGA TCAGCAGCAG 
GAACCTCTGT ACGGTGGAAC CCGCTCACTG TACTGCCGTT CGCCCACTCT GACCAGAAGC 
AACCTGAATC GCTCGCAATC TGTGTACGCC AAGAGCAACA CGGCCATCAA TCGAGACATT 
GTGCCGCGTC CAGGTCCTCT GGTGCCCGCC CAGAGTCTTT ATCCCATGCG CACCCAGCAG 
CAGCAGCAAC AGCAACAACA GCAACAGCAA CAAGTGGCAC CCGCACCTCA ATCTTCCCAT 
TTACAGAACC AAAATGTCCA GAATCAGATG CAGCAGCGCA GCGAGAGCAT CTACGGAATG 
AGGGGCTCCA TGCGCGGACA GCAGCAGCCA ATCCAGCAGC AACAACAACA GCAGCAGCAG 
CAGCAGCTTC AGCAACAACA GCCCAACATG GGAGTGCAGC AGCAACAGAT GCAGCCTCCG 
CCACAGATGA TGTCCGATCC TCAGCAGCAG CCGCAGGGCT TCCAGCCAGT CTACGGCACA 
CGCACAAATC CGACGCCGAT GGACGGAAAT CACAAGTATG ACCGACGTGA CCCGCAGCAA 
ATGTACGGCG TGACGGGACC AAGGAATCGC GGACAGTCGG CGCAGTCGGA CGACAGCTCC 
TATGGCTCAT ATCACGGCTC GGCTGTCACG CCGCCAGCTC GTCATCCCAG CGTGGAGCCA 
TCACCTCCGC CACCGCCCAT GCTGATGTAT GCCCCGCCCC CACAGCCGAA TGCAGCCCAC 
CCGCAACCCA TTCGCACGCA GTCGGAGCGA AAAGTTAGTG CTCCAGTTGT GGTATCCCAG 
CCGGCAGCGT GCGCCGTGAC CTACACAACC TCGCAAGGAT CAGCGGTGAC AGCCCAACAG 
CAGCAACAAC AACAGCAGCA GCAGCAGCAA CAACAGCAGC AGCAGCAGCA ACAGATGATG 
ATGCAACAAC AGCAGCAACA TTATGGAATG CTGCCGCTGA GGCCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476837.1
CDS	306..2396
Translation	MADESLHTVPLEHNIDYHIVTLFERLEAMRKDSHGGGHGVNNRLSSTLQAPKRSMQAEIRTLEFWRSIISECLASFMYVFIVCGAAAGVGVGASVSSVLLATALASGLAMATLTQCFLHISGAHINPAVTLALCVVRSISPIRAAMYITAQCGGGIAGAALLYGVTVPGYQGNLQAAISHSAALAAWERFGVEFILTFLVVLCYFVSTDPMKKFMGNSAASIGCAYSACCFVSMPYLNPARSLGPSFVLNKWDSHWVYWFGPLVGGMASGLVYEYIFNSRNRNLRHNKGSIDNDSSSIHSEDELNYDMDMEKPNKYQQSQGTYPRGQSNGNGGGQAAGNGQHQAANMGQMPGVVANAGQGNYCQNLYTAPPLSSKYDQQQEPLYGGTRSLYCRSPTLTRSNLNRSQSVYAKSNTAINRDIVPRPGPLVPAQSLYPMRTQQQQQQQQQQQQQVAPAPQSSHLQNQNVQNQMQQRSESIYGMRGSMRGQQQPIQQQQQQQQQQQLQQQQPNMGVQQQQMQPPPQMMSDPQQQPQGFQPVYGTRTNPTPMDGNHKYDRRDPQQMYGVTGPRNRGQSAQSDDSSYGSYHGSAVTPPARHPSVEPSPPPPPMLMYAPPPQPNAAHPQPIRTQSERKVSAPVVVSQPAACAVTYTTSQGSAVTAQQQQQQQQQQQQQQQQQQQQMMMQQQQQHYGMLPLRPN

Target ORF information:

RefSeq Version	NM_057489.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster big brain (bib), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057489.5

ORF Insert Sequence:

ATGGCCGACG AAAGTCTGCA CACCGTGCCC CTGGAGCACA ACATAGACTA CCACATCGTG 
ACGCTATTCG AGCGCCTGGA GGCGATGCGT AAGGACTCGC ACGGCGGTGG CCACGGGGTC 
AACAATCGCC TGTCCAGCAC CCTGCAGGCT CCCAAGCGGA GCATGCAGGC TGAGATTCGC 
ACGCTGGAGT TTTGGAGATC CATCATCAGC GAGTGTCTGG CCTCCTTCAT GTACGTGTTC 
ATCGTCTGCG GTGCCGCCGC TGGCGTCGGA GTGGGGGCCA GTGTGTCGTC CGTGCTTTTG 
GCCACGGCTC TGGCCTCCGG CCTGGCGATG GCTACACTGA CGCAGTGCTT CCTCCACATC 
TCGGGCGCCC ACATCAATCC CGCCGTAACC CTGGCTCTAT GCGTAGTGCG ATCCATATCT 
CCGATCCGGG CTGCCATGTA CATAACCGCA CAATGTGGCG GAGGAATCGC CGGAGCTGCT 
CTGCTTTATG GCGTAACTGT GCCTGGTTAC CAGGGGAATC TGCAGGCCGC CATCTCACAT 
AGCGCTGCTC TGGCCGCCTG GGAAAGATTC GGTGTGGAAT TCATCCTCAC CTTTCTGGTG 
GTTCTGTGCT ATTTCGTATC CACGGATCCC ATGAAGAAGT TCATGGGCAA TTCGGCCGCC 
TCGATTGGAT GTGCTTACAG CGCCTGCTGC TTTGTGTCGA TGCCGTACCT GAATCCAGCC 
CGCTCCCTGG GTCCTTCGTT TGTGCTTAAC AAATGGGACA GCCATTGGGT GTACTGGTTC 
GGACCACTGG TGGGCGGCAT GGCCTCTGGC CTGGTGTACG AGTACATCTT CAACTCGCGC 
AACCGCAACC TGCGCCACAA CAAGGGCAGC ATCGACAACG ATTCCAGTTC GATTCACTCG 
GAGGACGAAC TGAACTACGA CATGGATATG GAGAAGCCCA ACAAGTATCA GCAGTCGCAG 
GGCACCTACC CGCGTGGCCA GTCCAATGGC AATGGCGGAG GACAGGCGGC CGGAAATGGT 
CAGCACCAAG CTGCCAACAT GGGCCAGATG CCGGGCGTAG TGGCCAATGC CGGTCAGGGC 
AATTACTGCC AGAATCTGTA CACTGCTCCG CCGCTCTCCT CGAAGTACGA TCAGCAGCAG 
GAACCTCTGT ACGGTGGAAC CCGCTCACTG TACTGCCGTT CGCCCACTCT GACCAGAAGC 
AACCTGAATC GCTCGCAATC TGTGTACGCC AAGAGCAACA CGGCCATCAA TCGAGACATT 
GTGCCGCGTC CAGGTCCTCT GGTGCCCGCC CAGAGTCTTT ATCCCATGCG CACCCAGCAG 
CAGCAGCAAC AGCAACAACA GCAACAGCAA CAAGTGGCAC CCGCACCTCA ATCTTCCCAT 
TTACAGAACC AAAATGTCCA GAATCAGATG CAGCAGCGCA GCGAGAGCAT CTACGGAATG 
AGGGGCTCCA TGCGCGGACA GCAGCAGCCA ATCCAGCAGC AACAACAACA GCAGCAGCAG 
CAGCAGCTTC AGCAACAACA GCCCAACATG GGAGTGCAGC AGCAACAGAT GCAGCCTCCG 
CCACAGATGA TGTCCGATCC TCAGCAGCAG CCGCAGGGCT TCCAGCCAGT CTACGGCACA 
CGCACAAATC CGACGCCGAT GGACGGAAAT CACAAGTATG ACCGACGTGA CCCGCAGCAA 
ATGTACGGCG TGACGGGACC AAGGAATCGC GGACAGTCGG CGCAGTCGGA CGACAGCTCC 
TATGGCTCAT ATCACGGCTC GGCTGTCACG CCGCCAGCTC GTCATCCCAG CGTGGAGCCA 
TCACCTCCGC CACCGCCCAT GCTGATGTAT GCCCCGCCCC CACAGCCGAA TGCAGCCCAC 
CCGCAACCCA TTCGCACGCA GTCGGAGCGA AAAGTTAGTG CTCCAGTTGT GGTATCCCAG 
CCGGCAGCGT GCGCCGTGAC CTACACAACC TCGCAAGGAT CAGCGGTGAC AGCCCAACAG 
CAGCAACAAC AACAGCAGCA GCAGCAGCAA CAACAGCAGC AGCAGCAGCA ACAGATGATG 
ATGCAACAAC AGCAGCAACA TTATGGAATG CTGCCGCTGA GGCCCAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bib ( NM_001273384.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260313.1 Drosophila melanogaster big brain (bib), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15968
Clone ID Related Accession (Same CDS sequence)	NM_001273384.2
Accession Version	NM_001273384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	big brain, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273384.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGACG AAAGTCTGCA CACCGTGCCC CTGGAGCACA ACATAGACTA CCACATCGTG 
ACGCTATTCG AGCGCCTGGA GGCGATGCGT AAGGACTCGC ACGGCGGTGG CCACGGGGTC 
AACAATCGCC TGTCCAGCAC CCTGCAGGCT CCCAAGCGGA GCATGCAGGC TGAGATTCGC 
ACGCTGGAGT TTTGGAGATC CATCATCAGC GAGTGTCTGG CCTCCTTCAT GTACGTGTTC 
ATCGTCTGCG GTGCCGCCGC TGGCGTCGGA GTGGGGGCCA GTGTGTCGTC CGTGCTTTTG 
GCCACGGCTC TGGCCTCCGG CCTGGCGATG GCTACACTGA CGCAGTGCTT CCTCCACATC 
TCGGGCGCCC ACATCAATCC CGCCGTAACC CTGGCTCTAT GCGTAGTGCG ATCCATATCT 
CCGATCCGGG CTGCCATGTA CATAACCGCA CAATGTGGCG GAGGAATCGC CGGAGCTGCT 
CTGCTTTATG GCGTAACTGT GCCTGGTTAC CAGGGGAATC TGCAGGCCGC CATCTCACAT 
AGCGCTGCTC TGGCCGCCTG GGAAAGATTC GGTGTGGAAT TCATCCTCAC CTTTCTGGTG 
GTTCTGTGCT ATTTCGTATC CACGGATCCC ATGAAGAAGT TCATGGGCAA TTCGGCCGCC 
TCGATTGGAT GTGCTTACAG CGCCTGCTGC TTTGTGTCGA TGCCGTACCT GAATCCAGCC 
CGCTCCCTGG GTCCTTCGTT TGTGCTTAAC AAATGGGACA GCCATTGGGT GTACTGGTTC 
GGACCACTGG TGGGCGGCAT GGCCTCTGGC CTGGTGTACG AGTACATCTT CAACTCGCGC 
AACCGCAACC TGCGCCACAA CAAGGGCAGC ATCGACAACG ATTCCAGTTC GATTCACTCG 
GAGGACGAAC TGAACTACGA CATGGATATG GAGAAGCCCA ACAAGTATCA GCAGTCGCAG 
GGCACCTACC CGCGTGGCCA GTCCAATGGC AATGGCGGAG GACAGGCGGC CGGAAATGGT 
CAGCACCAAG CTGCCAACAT GGGCCAGATG CCGGGCGTAG TGGCCAATGC CGGTCAGGGC 
AATTACTGCC AGAATCTGTA CACTGCTCCG CCGCTCTCCT CGAAGTACGA TCAGCAGCAG 
GAACCTCTGT ACGGTGGAAC CCGCTCACTG TACTGCCGTT CGCCCACTCT GACCAGAAGC 
AACCTGAATC GCTCGCAATC TGTGTACGCC AAGAGCAACA CGGCCATCAA TCGAGACATT 
GTGCCGCGTC CAGGTCCTCT GGTGCCCGCC CAGAGTCTTT ATCCCATGCG CACCCAGCAG 
CAGCAGCAAC AGCAACAACA GCAACAGCAA CAAGTGGCAC CCGCACCTCA ATCTTCCCAT 
TTACAGAACC AAAATGTCCA GAATCAGATG CAGCAGCGCA GCGAGAGCAT CTACGGAATG 
AGGGGCTCCA TGCGCGGACA GCAGCAGCCA ATCCAGCAGC AACAACAACA GCAGCAGCAG 
CAGCAGCTTC AGCAACAACA GCCCAACATG GGAGTGCAGC AGCAACAGAT GCAGCCTCCG 
CCACAGATGA TGTCCGATCC TCAGCAGCAG CCGCAGGGCT TCCAGCCAGT CTACGGCACA 
CGCACAAATC CGACGCCGAT GGACGGAAAT CACAAGTATG ACCGACGTGA CCCGCAGCAA 
ATGTACGGCG TGACGGGACC AAGGAATCGC GGACAGTCGG CGCAGTCGGA CGACAGCTCC 
TATGGCTCAT ATCACGGCTC GGCTGTCACG CCGCCAGCTC GTCATCCCAG CGTGGAGCCA 
TCACCTCCGC CACCGCCCAT GCTGATGTAT GCCCCGCCCC CACAGCCGAA TGCAGCCCAC 
CCGCAACCCA TTCGCACGCA GTCGGAGCGA AAAGTTAGTG CTCCAGTTGT GGTATCCCAG 
CCGGCAGCGT GCGCCGTGAC CTACACAACC TCGCAAGGAT CAGCGGTGAC AGCCCAACAG 
CAGCAACAAC AACAGCAGCA GCAGCAGCAA CAACAGCAGC AGCAGCAGCA ACAGATGATG 
ATGCAACAAC AGCAGCAACA TTATGGAATG CTGCCGCTGA GGCCCAACTG AAAGCGGCTG 
TCATGGGGTC CGGTGACAAC GCCGCCGCCA GGGATCCACG CCCTCGCCCT CCAGCCCGGA 
GGCACCATGA CCCTGCACCG CTGCAGCCTA GCCAAGCAGA GCAGTTTGTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260313.1
CDS	306..2519
Translation	MADESLHTVPLEHNIDYHIVTLFERLEAMRKDSHGGGHGVNNRLSSTLQAPKRSMQAEIRTLEFWRSIISECLASFMYVFIVCGAAAGVGVGASVSSVLLATALASGLAMATLTQCFLHISGAHINPAVTLALCVVRSISPIRAAMYITAQCGGGIAGAALLYGVTVPGYQGNLQAAISHSAALAAWERFGVEFILTFLVVLCYFVSTDPMKKFMGNSAASIGCAYSACCFVSMPYLNPARSLGPSFVLNKWDSHWVYWFGPLVGGMASGLVYEYIFNSRNRNLRHNKGSIDNDSSSIHSEDELNYDMDMEKPNKYQQSQGTYPRGQSNGNGGGQAAGNGQHQAANMGQMPGVVANAGQGNYCQNLYTAPPLSSKYDQQQEPLYGGTRSLYCRSPTLTRSNLNRSQSVYAKSNTAINRDIVPRPGPLVPAQSLYPMRTQQQQQQQQQQQQQVAPAPQSSHLQNQNVQNQMQQRSESIYGMRGSMRGQQQPIQQQQQQQQQQQLQQQQPNMGVQQQQMQPPPQMMSDPQQQPQGFQPVYGTRTNPTPMDGNHKYDRRDPQQMYGVTGPRNRGQSAQSDDSSYGSYHGSAVTPPARHPSVEPSPPPPPMLMYAPPPQPNAAHPQPIRTQSERKVSAPVVVSQPAACAVTYTTSQGSAVTAQQQQQQQQQQQQQQQQQQQQMMMQQQQQHYGMLPLRPN*KRLSWGPVTTPPPGIHALALQPGGTMTLHRCSLAKQSSLF

Target ORF information:

RefSeq Version	NM_001273384.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster big brain (bib), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273384.2

ORF Insert Sequence:

ATGGCCGACG AAAGTCTGCA CACCGTGCCC CTGGAGCACA ACATAGACTA CCACATCGTG 
ACGCTATTCG AGCGCCTGGA GGCGATGCGT AAGGACTCGC ACGGCGGTGG CCACGGGGTC 
AACAATCGCC TGTCCAGCAC CCTGCAGGCT CCCAAGCGGA GCATGCAGGC TGAGATTCGC 
ACGCTGGAGT TTTGGAGATC CATCATCAGC GAGTGTCTGG CCTCCTTCAT GTACGTGTTC 
ATCGTCTGCG GTGCCGCCGC TGGCGTCGGA GTGGGGGCCA GTGTGTCGTC CGTGCTTTTG 
GCCACGGCTC TGGCCTCCGG CCTGGCGATG GCTACACTGA CGCAGTGCTT CCTCCACATC 
TCGGGCGCCC ACATCAATCC CGCCGTAACC CTGGCTCTAT GCGTAGTGCG ATCCATATCT 
CCGATCCGGG CTGCCATGTA CATAACCGCA CAATGTGGCG GAGGAATCGC CGGAGCTGCT 
CTGCTTTATG GCGTAACTGT GCCTGGTTAC CAGGGGAATC TGCAGGCCGC CATCTCACAT 
AGCGCTGCTC TGGCCGCCTG GGAAAGATTC GGTGTGGAAT TCATCCTCAC CTTTCTGGTG 
GTTCTGTGCT ATTTCGTATC CACGGATCCC ATGAAGAAGT TCATGGGCAA TTCGGCCGCC 
TCGATTGGAT GTGCTTACAG CGCCTGCTGC TTTGTGTCGA TGCCGTACCT GAATCCAGCC 
CGCTCCCTGG GTCCTTCGTT TGTGCTTAAC AAATGGGACA GCCATTGGGT GTACTGGTTC 
GGACCACTGG TGGGCGGCAT GGCCTCTGGC CTGGTGTACG AGTACATCTT CAACTCGCGC 
AACCGCAACC TGCGCCACAA CAAGGGCAGC ATCGACAACG ATTCCAGTTC GATTCACTCG 
GAGGACGAAC TGAACTACGA CATGGATATG GAGAAGCCCA ACAAGTATCA GCAGTCGCAG 
GGCACCTACC CGCGTGGCCA GTCCAATGGC AATGGCGGAG GACAGGCGGC CGGAAATGGT 
CAGCACCAAG CTGCCAACAT GGGCCAGATG CCGGGCGTAG TGGCCAATGC CGGTCAGGGC 
AATTACTGCC AGAATCTGTA CACTGCTCCG CCGCTCTCCT CGAAGTACGA TCAGCAGCAG 
GAACCTCTGT ACGGTGGAAC CCGCTCACTG TACTGCCGTT CGCCCACTCT GACCAGAAGC 
AACCTGAATC GCTCGCAATC TGTGTACGCC AAGAGCAACA CGGCCATCAA TCGAGACATT 
GTGCCGCGTC CAGGTCCTCT GGTGCCCGCC CAGAGTCTTT ATCCCATGCG CACCCAGCAG 
CAGCAGCAAC AGCAACAACA GCAACAGCAA CAAGTGGCAC CCGCACCTCA ATCTTCCCAT 
TTACAGAACC AAAATGTCCA GAATCAGATG CAGCAGCGCA GCGAGAGCAT CTACGGAATG 
AGGGGCTCCA TGCGCGGACA GCAGCAGCCA ATCCAGCAGC AACAACAACA GCAGCAGCAG 
CAGCAGCTTC AGCAACAACA GCCCAACATG GGAGTGCAGC AGCAACAGAT GCAGCCTCCG 
CCACAGATGA TGTCCGATCC TCAGCAGCAG CCGCAGGGCT TCCAGCCAGT CTACGGCACA 
CGCACAAATC CGACGCCGAT GGACGGAAAT CACAAGTATG ACCGACGTGA CCCGCAGCAA 
ATGTACGGCG TGACGGGACC AAGGAATCGC GGACAGTCGG CGCAGTCGGA CGACAGCTCC 
TATGGCTCAT ATCACGGCTC GGCTGTCACG CCGCCAGCTC GTCATCCCAG CGTGGAGCCA 
TCACCTCCGC CACCGCCCAT GCTGATGTAT GCCCCGCCCC CACAGCCGAA TGCAGCCCAC 
CCGCAACCCA TTCGCACGCA GTCGGAGCGA AAAGTTAGTG CTCCAGTTGT GGTATCCCAG 
CCGGCAGCGT GCGCCGTGAC CTACACAACC TCGCAAGGAT CAGCGGTGAC AGCCCAACAG 
CAGCAACAAC AACAGCAGCA GCAGCAGCAA CAACAGCAGC AGCAGCAGCA ACAGATGATG 
ATGCAACAAC AGCAGCAACA TTATGGAATG CTGCCGCTGA GGCCCAACTG AAAGCGGCTG 
TCATGGGGTC CGGTGACAAC GCCGCCGCCA GGGATCCACG CCCTCGCCCT CCAGCCCGGA 
GGCACCATGA CCCTGCACCG CTGCAGCCTA GCCAAGCAGA GCAGTTTGTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bib cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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