CG5853 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG5853
Entrez Gene ID	34322
Full Name	CG5853 gene product from transcript CG5853-RC
Synonyms	Dmel\CG5853
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 30F1-30F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_176005.2	NP_788019.1	uncharacterized protein, isoform A
NM_001273380.1	NP_001260309.1	uncharacterized protein, isoform B
NM_001298857.1	NP_001285786.1	uncharacterized protein, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG5853	NP_001260309.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009463	XP_310233.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG5853 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG5853 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14253	NM_001298857.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG5853), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa14253	NM_001273380.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5853), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa14253	NM_176005.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5853), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG5853 ( NM_176005.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788019.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG5853), mRNA.

Price & Availability↑

CloneID	OFa14253
Clone ID Related Accession (Same CDS sequence)	NM_176005.2 , NM_001273380.1 , NM_001298857.1
Accession Version	NM_176005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_176005.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGACAC CGCGCGAGCA GTTCTTGTTC GGCAGCGACA ACGGAGTGGC CGGCCTCGGT 
CTGGGCATCG AGATCGGGGA GGTGACCTCC AGTCGCGTCT ATAACAGCAC CACAGTGCCA 
GTTGGCAACA GTGGCGGATG GCGGAATGCC CAGTCCAGTG TTACGCCACC ACCCATACCC 
ACACCGCCAC AGGTGAACTA TACCAATGGG AATGAGCCCA GCAAGCAGCC GCCACTGGAA 
CCTGTCGTGG AGGAGGAGGT GCACTTCGAC ACCGATGCCC TCAATAATCT GCCAGCTCGC 
GAGCCTGTGG ACATGGAGTT CAAGGAGCTG TCCCTCACCG TCAAATTGGG CTTCAATCGA 
GGATCCAAGG AAATCTTGCA CAATGTGTGC GGAAAGTTCC CAGGCAGCCA GCTGATTGCC 
ATCATGGGAC CTTCGGGTGC AGGAAAGTCC ACTTTGCTAG ATGCCCTTTC AGGTTTCAAG 
ACGACCGGAG TGGATGGCTC CATCTTGCTC AATGGTCGGC GACGTGATTT GCCTTCCTTC 
CGTCGGATGT CCTGCTACAT CACCCAGGAT GATCGTCTGC AGCCGCTGCT GACGGTAAAC 
GAGAATATGC ATATAGCCGC CGATCTTAAA CTGGGTCAGA CAGTCAGCTA CGAGGAGAAG 
GAGAGCAGGA TCGAGGACAT TCTGCTGCTC CTTGGTCTGT ACAATCACGA TCAGACCCTT 
ACAATGCGCC TGTCCGGTGG TCAGAAGAAG AGGCTATCCA TTGCCATGGA GTTGATCAAT 
AATCCCACTG TGATGTTCCT GGACGAGCCC ACAACTGGCT TGGACAGTAG CTCGTGCACA 
AAGGTCCTGG AGCTGCTCAA GAAGTTGACC AGCCAAGGAC GCACCATTAT TTGCACCATC 
CATCAGCCGA CGGCCAAGTT GTTCCAGATC TTCGATCAGG TCTACGTCCT GTCCGCTGGC 
AACTGCGTCT ACCAGGGCAG CACCCAGAAA TTGGTGCCGT TCCTGCAGTC GGTGGATCTG 
CCCTGTCCGA TGTACCACAA TCCAGCGGAT TACATTATCG AGCTCGCTTG CGGAGAGTAT 
GGATACGACA AGGTCGACAC CCTTAAGCTC GCCACTGAGA ACGGAAGCTG CCTGACTTGG 
TTCCATAATC CCAGTGCGGT GTTGCGCGCT GAGGTCTTGA TGAGAAAGTA TCCCATACCA 
AAGAAAACCA AAAGCCGATC CCTGGAGGAC ACCAGCTACT CAAACCAGTG CTCTGTGCTT 
TTGCGACGAG GTTTCATCAA AGCCAAACGA GACACGACCA TGACGCACTT GAGGATCGGA 
GTCAACATAG CAGTGGCCGC TCTGTTCGGA GCTATGTACG ATCACACGGG ACGTGAAGGA 
TCACGAGTGC TCGACAACTA CAATCTTCTG TTTGCCATAC TGATGCACCA TTCCATGACC 
ACGATGATGT TGACTGTTCT GACTTTCCCC ATGGACATCT CCATATTGAT CAAGGAGCAC 
TTCAATCGCT GGTACTCACT CAAAGCCTAC TACACTGCCA TGACACTCGT GGACCTGCCC 
ATATCTATCA TAAGTTGTTT CTTCTTCACT GTCATCGTTT ACCTTTGGAG TTATCAGCCG 
ATGGAGTGGA TCAGATTCTT TATGTTCTTC TCCATTAGTT TACTGACCGT CTTTGTGGGA 
CATAGTTTTG GTTTGATGAT TGGTGCCTGG TTCGATGTGG TGAATGGAAC GTTCTTGGCC 
CCAGTGCTAA CGATTCCCAT GATGATGTTC GCCGGCTTTG GAGTGACCCT GCGTGATCTG 
CCAAGCTACT TAAGGTGGGG CAGTCACATA TCATACTTAA GATATGGACT TGAGGGCTTC 
ATATCAGCCA TTTATGGCTT GGATCGAGGT ACCTTGGCCT GCGAGGAGGC GCCGTACTGC 
CATTACAGGT ATCCAAAGAA ATTCTTAGAG GAAATCACCA TGAGGGGTGA TCAGTTCTGG 
AACGATGTGA TCGCGCTGGG AGTCATGATC CTGGTTTTCC GATTTGTGTC CTACGTGGTG 
CTGAAGGCCA AGATCAAGTC GATCCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788019.1
CDS	169..2238
Translation	MATPREQFLFGSDNGVAGLGLGIEIGEVTSSRVYNSTTVPVGNSGGWRNAQSSVTPPPIPTPPQVNYTNGNEPSKQPPLEPVVEEEVHFDTDALNNLPAREPVDMEFKELSLTVKLGFNRGSKEILHNVCGKFPGSQLIAIMGPSGAGKSTLLDALSGFKTTGVDGSILLNGRRRDLPSFRRMSCYITQDDRLQPLLTVNENMHIAADLKLGQTVSYEEKESRIEDILLLLGLYNHDQTLTMRLSGGQKKRLSIAMELINNPTVMFLDEPTTGLDSSSCTKVLELLKKLTSQGRTIICTIHQPTAKLFQIFDQVYVLSAGNCVYQGSTQKLVPFLQSVDLPCPMYHNPADYIIELACGEYGYDKVDTLKLATENGSCLTWFHNPSAVLRAEVLMRKYPIPKKTKSRSLEDTSYSNQCSVLLRRGFIKAKRDTTMTHLRIGVNIAVAALFGAMYDHTGREGSRVLDNYNLLFAILMHHSMTTMMLTVLTFPMDISILIKEHFNRWYSLKAYYTAMTLVDLPISIISCFFFTVIVYLWSYQPMEWIRFFMFFSISLLTVFVGHSFGLMIGAWFDVVNGTFLAPVLTIPMMMFAGFGVTLRDLPSYLRWGSHISYLRYGLEGFISAIYGLDRGTLACEEAPYCHYRYPKKFLEEITMRGDQFWNDVIALGVMILVFRFVSYVVLKAKIKSIR

Target ORF information:

RefSeq Version	NM_176005.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5853), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176005.2

ORF Insert Sequence:

ATGGCGACAC CGCGCGAGCA GTTCTTGTTC GGCAGCGACA ACGGAGTGGC CGGCCTCGGT 
CTGGGCATCG AGATCGGGGA GGTGACCTCC AGTCGCGTCT ATAACAGCAC CACAGTGCCA 
GTTGGCAACA GTGGCGGATG GCGGAATGCC CAGTCCAGTG TTACGCCACC ACCCATACCC 
ACACCGCCAC AGGTGAACTA TACCAATGGG AATGAGCCCA GCAAGCAGCC GCCACTGGAA 
CCTGTCGTGG AGGAGGAGGT GCACTTCGAC ACCGATGCCC TCAATAATCT GCCAGCTCGC 
GAGCCTGTGG ACATGGAGTT CAAGGAGCTG TCCCTCACCG TCAAATTGGG CTTCAATCGA 
GGATCCAAGG AAATCTTGCA CAATGTGTGC GGAAAGTTCC CAGGCAGCCA GCTGATTGCC 
ATCATGGGAC CTTCGGGTGC AGGAAAGTCC ACTTTGCTAG ATGCCCTTTC AGGTTTCAAG 
ACGACCGGAG TGGATGGCTC CATCTTGCTC AATGGTCGGC GACGTGATTT GCCTTCCTTC 
CGTCGGATGT CCTGCTACAT CACCCAGGAT GATCGTCTGC AGCCGCTGCT GACGGTAAAC 
GAGAATATGC ATATAGCCGC CGATCTTAAA CTGGGTCAGA CAGTCAGCTA CGAGGAGAAG 
GAGAGCAGGA TCGAGGACAT TCTGCTGCTC CTTGGTCTGT ACAATCACGA TCAGACCCTT 
ACAATGCGCC TGTCCGGTGG TCAGAAGAAG AGGCTATCCA TTGCCATGGA GTTGATCAAT 
AATCCCACTG TGATGTTCCT GGACGAGCCC ACAACTGGCT TGGACAGTAG CTCGTGCACA 
AAGGTCCTGG AGCTGCTCAA GAAGTTGACC AGCCAAGGAC GCACCATTAT TTGCACCATC 
CATCAGCCGA CGGCCAAGTT GTTCCAGATC TTCGATCAGG TCTACGTCCT GTCCGCTGGC 
AACTGCGTCT ACCAGGGCAG CACCCAGAAA TTGGTGCCGT TCCTGCAGTC GGTGGATCTG 
CCCTGTCCGA TGTACCACAA TCCAGCGGAT TACATTATCG AGCTCGCTTG CGGAGAGTAT 
GGATACGACA AGGTCGACAC CCTTAAGCTC GCCACTGAGA ACGGAAGCTG CCTGACTTGG 
TTCCATAATC CCAGTGCGGT GTTGCGCGCT GAGGTCTTGA TGAGAAAGTA TCCCATACCA 
AAGAAAACCA AAAGCCGATC CCTGGAGGAC ACCAGCTACT CAAACCAGTG CTCTGTGCTT 
TTGCGACGAG GTTTCATCAA AGCCAAACGA GACACGACCA TGACGCACTT GAGGATCGGA 
GTCAACATAG CAGTGGCCGC TCTGTTCGGA GCTATGTACG ATCACACGGG ACGTGAAGGA 
TCACGAGTGC TCGACAACTA CAATCTTCTG TTTGCCATAC TGATGCACCA TTCCATGACC 
ACGATGATGT TGACTGTTCT GACTTTCCCC ATGGACATCT CCATATTGAT CAAGGAGCAC 
TTCAATCGCT GGTACTCACT CAAAGCCTAC TACACTGCCA TGACACTCGT GGACCTGCCC 
ATATCTATCA TAAGTTGTTT CTTCTTCACT GTCATCGTTT ACCTTTGGAG TTATCAGCCG 
ATGGAGTGGA TCAGATTCTT TATGTTCTTC TCCATTAGTT TACTGACCGT CTTTGTGGGA 
CATAGTTTTG GTTTGATGAT TGGTGCCTGG TTCGATGTGG TGAATGGAAC GTTCTTGGCC 
CCAGTGCTAA CGATTCCCAT GATGATGTTC GCCGGCTTTG GAGTGACCCT GCGTGATCTG 
CCAAGCTACT TAAGGTGGGG CAGTCACATA TCATACTTAA GATATGGACT TGAGGGCTTC 
ATATCAGCCA TTTATGGCTT GGATCGAGGT ACCTTGGCCT GCGAGGAGGC GCCGTACTGC 
CATTACAGGT ATCCAAAGAA ATTCTTAGAG GAAATCACCA TGAGGGGTGA TCAGTTCTGG 
AACGATGTGA TCGCGCTGGG AGTCATGATC CTGGTTTTCC GATTTGTGTC CTACGTGGTG 
CTGAAGGCCA AGATCAAGTC GATCCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG5853 ( NM_001273380.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260309.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG5853), mRNA.

Price & Availability↑

CloneID	OFa14253
Clone ID Related Accession (Same CDS sequence)	NM_176005.2 , NM_001273380.1 , NM_001298857.1
Accession Version	NM_001273380.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGACAC CGCGCGAGCA GTTCTTGTTC GGCAGCGACA ACGGAGTGGC CGGCCTCGGT 
CTGGGCATCG AGATCGGGGA GGTGACCTCC AGTCGCGTCT ATAACAGCAC CACAGTGCCA 
GTTGGCAACA GTGGCGGATG GCGGAATGCC CAGTCCAGTG TTACGCCACC ACCCATACCC 
ACACCGCCAC AGGTGAACTA TACCAATGGG AATGAGCCCA GCAAGCAGCC GCCACTGGAA 
CCTGTCGTGG AGGAGGAGGT GCACTTCGAC ACCGATGCCC TCAATAATCT GCCAGCTCGC 
GAGCCTGTGG ACATGGAGTT CAAGGAGCTG TCCCTCACCG TCAAATTGGG CTTCAATCGA 
GGATCCAAGG AAATCTTGCA CAATGTGTGC GGAAAGTTCC CAGGCAGCCA GCTGATTGCC 
ATCATGGGAC CTTCGGGTGC AGGAAAGTCC ACTTTGCTAG ATGCCCTTTC AGGTTTCAAG 
ACGACCGGAG TGGATGGCTC CATCTTGCTC AATGGTCGGC GACGTGATTT GCCTTCCTTC 
CGTCGGATGT CCTGCTACAT CACCCAGGAT GATCGTCTGC AGCCGCTGCT GACGGTAAAC 
GAGAATATGC ATATAGCCGC CGATCTTAAA CTGGGTCAGA CAGTCAGCTA CGAGGAGAAG 
GAGAGCAGGA TCGAGGACAT TCTGCTGCTC CTTGGTCTGT ACAATCACGA TCAGACCCTT 
ACAATGCGCC TGTCCGGTGG TCAGAAGAAG AGGCTATCCA TTGCCATGGA GTTGATCAAT 
AATCCCACTG TGATGTTCCT GGACGAGCCC ACAACTGGCT TGGACAGTAG CTCGTGCACA 
AAGGTCCTGG AGCTGCTCAA GAAGTTGACC AGCCAAGGAC GCACCATTAT TTGCACCATC 
CATCAGCCGA CGGCCAAGTT GTTCCAGATC TTCGATCAGG TCTACGTCCT GTCCGCTGGC 
AACTGCGTCT ACCAGGGCAG CACCCAGAAA TTGGTGCCGT TCCTGCAGTC GGTGGATCTG 
CCCTGTCCGA TGTACCACAA TCCAGCGGAT TACATTATCG AGCTCGCTTG CGGAGAGTAT 
GGATACGACA AGGTCGACAC CCTTAAGCTC GCCACTGAGA ACGGAAGCTG CCTGACTTGG 
TTCCATAATC CCAGTGCGGT GTTGCGCGCT GAGGTCTTGA TGAGAAAGTA TCCCATACCA 
AAGAAAACCA AAAGCCGATC CCTGGAGGAC ACCAGCTACT CAAACCAGTG CTCTGTGCTT 
TTGCGACGAG GTTTCATCAA AGCCAAACGA GACACGACCA TGACGCACTT GAGGATCGGA 
GTCAACATAG CAGTGGCCGC TCTGTTCGGA GCTATGTACG ATCACACGGG ACGTGAAGGA 
TCACGAGTGC TCGACAACTA CAATCTTCTG TTTGCCATAC TGATGCACCA TTCCATGACC 
ACGATGATGT TGACTGTTCT GACTTTCCCC ATGGACATCT CCATATTGAT CAAGGAGCAC 
TTCAATCGCT GGTACTCACT CAAAGCCTAC TACACTGCCA TGACACTCGT GGACCTGCCC 
ATATCTATCA TAAGTTGTTT CTTCTTCACT GTCATCGTTT ACCTTTGGAG TTATCAGCCG 
ATGGAGTGGA TCAGATTCTT TATGTTCTTC TCCATTAGTT TACTGACCGT CTTTGTGGGA 
CATAGTTTTG GTTTGATGAT TGGTGCCTGG TTCGATGTGG TGAATGGAAC GTTCTTGGCC 
CCAGTGCTAA CGATTCCCAT GATGATGTTC GCCGGCTTTG GAGTGACCCT GCGTGATCTG 
CCAAGCTACT TAAGGTGGGG CAGTCACATA TCATACTTAA GATATGGACT TGAGGGCTTC 
ATATCAGCCA TTTATGGCTT GGATCGAGGT ACCTTGGCCT GCGAGGAGGC GCCGTACTGC 
CATTACAGGT ATCCAAAGAA ATTCTTAGAG GAAATCACCA TGAGGGGTGA TCAGTTCTGG 
AACGATGTGA TCGCGCTGGG AGTCATGATC CTGGTTTTCC GATTTGTGTC CTACGTGGTG 
CTGAAGGCCA AGATCAAGTC GATCCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260309.1
CDS	143..2212
Translation	MATPREQFLFGSDNGVAGLGLGIEIGEVTSSRVYNSTTVPVGNSGGWRNAQSSVTPPPIPTPPQVNYTNGNEPSKQPPLEPVVEEEVHFDTDALNNLPAREPVDMEFKELSLTVKLGFNRGSKEILHNVCGKFPGSQLIAIMGPSGAGKSTLLDALSGFKTTGVDGSILLNGRRRDLPSFRRMSCYITQDDRLQPLLTVNENMHIAADLKLGQTVSYEEKESRIEDILLLLGLYNHDQTLTMRLSGGQKKRLSIAMELINNPTVMFLDEPTTGLDSSSCTKVLELLKKLTSQGRTIICTIHQPTAKLFQIFDQVYVLSAGNCVYQGSTQKLVPFLQSVDLPCPMYHNPADYIIELACGEYGYDKVDTLKLATENGSCLTWFHNPSAVLRAEVLMRKYPIPKKTKSRSLEDTSYSNQCSVLLRRGFIKAKRDTTMTHLRIGVNIAVAALFGAMYDHTGREGSRVLDNYNLLFAILMHHSMTTMMLTVLTFPMDISILIKEHFNRWYSLKAYYTAMTLVDLPISIISCFFFTVIVYLWSYQPMEWIRFFMFFSISLLTVFVGHSFGLMIGAWFDVVNGTFLAPVLTIPMMMFAGFGVTLRDLPSYLRWGSHISYLRYGLEGFISAIYGLDRGTLACEEAPYCHYRYPKKFLEEITMRGDQFWNDVIALGVMILVFRFVSYVVLKAKIKSIR

Target ORF information:

RefSeq Version	NM_001273380.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5853), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273380.1

ORF Insert Sequence:

ATGGCGACAC CGCGCGAGCA GTTCTTGTTC GGCAGCGACA ACGGAGTGGC CGGCCTCGGT 
CTGGGCATCG AGATCGGGGA GGTGACCTCC AGTCGCGTCT ATAACAGCAC CACAGTGCCA 
GTTGGCAACA GTGGCGGATG GCGGAATGCC CAGTCCAGTG TTACGCCACC ACCCATACCC 
ACACCGCCAC AGGTGAACTA TACCAATGGG AATGAGCCCA GCAAGCAGCC GCCACTGGAA 
CCTGTCGTGG AGGAGGAGGT GCACTTCGAC ACCGATGCCC TCAATAATCT GCCAGCTCGC 
GAGCCTGTGG ACATGGAGTT CAAGGAGCTG TCCCTCACCG TCAAATTGGG CTTCAATCGA 
GGATCCAAGG AAATCTTGCA CAATGTGTGC GGAAAGTTCC CAGGCAGCCA GCTGATTGCC 
ATCATGGGAC CTTCGGGTGC AGGAAAGTCC ACTTTGCTAG ATGCCCTTTC AGGTTTCAAG 
ACGACCGGAG TGGATGGCTC CATCTTGCTC AATGGTCGGC GACGTGATTT GCCTTCCTTC 
CGTCGGATGT CCTGCTACAT CACCCAGGAT GATCGTCTGC AGCCGCTGCT GACGGTAAAC 
GAGAATATGC ATATAGCCGC CGATCTTAAA CTGGGTCAGA CAGTCAGCTA CGAGGAGAAG 
GAGAGCAGGA TCGAGGACAT TCTGCTGCTC CTTGGTCTGT ACAATCACGA TCAGACCCTT 
ACAATGCGCC TGTCCGGTGG TCAGAAGAAG AGGCTATCCA TTGCCATGGA GTTGATCAAT 
AATCCCACTG TGATGTTCCT GGACGAGCCC ACAACTGGCT TGGACAGTAG CTCGTGCACA 
AAGGTCCTGG AGCTGCTCAA GAAGTTGACC AGCCAAGGAC GCACCATTAT TTGCACCATC 
CATCAGCCGA CGGCCAAGTT GTTCCAGATC TTCGATCAGG TCTACGTCCT GTCCGCTGGC 
AACTGCGTCT ACCAGGGCAG CACCCAGAAA TTGGTGCCGT TCCTGCAGTC GGTGGATCTG 
CCCTGTCCGA TGTACCACAA TCCAGCGGAT TACATTATCG AGCTCGCTTG CGGAGAGTAT 
GGATACGACA AGGTCGACAC CCTTAAGCTC GCCACTGAGA ACGGAAGCTG CCTGACTTGG 
TTCCATAATC CCAGTGCGGT GTTGCGCGCT GAGGTCTTGA TGAGAAAGTA TCCCATACCA 
AAGAAAACCA AAAGCCGATC CCTGGAGGAC ACCAGCTACT CAAACCAGTG CTCTGTGCTT 
TTGCGACGAG GTTTCATCAA AGCCAAACGA GACACGACCA TGACGCACTT GAGGATCGGA 
GTCAACATAG CAGTGGCCGC TCTGTTCGGA GCTATGTACG ATCACACGGG ACGTGAAGGA 
TCACGAGTGC TCGACAACTA CAATCTTCTG TTTGCCATAC TGATGCACCA TTCCATGACC 
ACGATGATGT TGACTGTTCT GACTTTCCCC ATGGACATCT CCATATTGAT CAAGGAGCAC 
TTCAATCGCT GGTACTCACT CAAAGCCTAC TACACTGCCA TGACACTCGT GGACCTGCCC 
ATATCTATCA TAAGTTGTTT CTTCTTCACT GTCATCGTTT ACCTTTGGAG TTATCAGCCG 
ATGGAGTGGA TCAGATTCTT TATGTTCTTC TCCATTAGTT TACTGACCGT CTTTGTGGGA 
CATAGTTTTG GTTTGATGAT TGGTGCCTGG TTCGATGTGG TGAATGGAAC GTTCTTGGCC 
CCAGTGCTAA CGATTCCCAT GATGATGTTC GCCGGCTTTG GAGTGACCCT GCGTGATCTG 
CCAAGCTACT TAAGGTGGGG CAGTCACATA TCATACTTAA GATATGGACT TGAGGGCTTC 
ATATCAGCCA TTTATGGCTT GGATCGAGGT ACCTTGGCCT GCGAGGAGGC GCCGTACTGC 
CATTACAGGT ATCCAAAGAA ATTCTTAGAG GAAATCACCA TGAGGGGTGA TCAGTTCTGG 
AACGATGTGA TCGCGCTGGG AGTCATGATC CTGGTTTTCC GATTTGTGTC CTACGTGGTG 
CTGAAGGCCA AGATCAAGTC GATCCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG5853 ( NM_001298857.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285786.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG5853), mRNA.

Price & Availability↑

CloneID	OFa14253
Clone ID Related Accession (Same CDS sequence)	NM_176005.2 , NM_001273380.1 , NM_001298857.1
Accession Version	NM_001298857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGACAC CGCGCGAGCA GTTCTTGTTC GGCAGCGACA ACGGAGTGGC CGGCCTCGGT 
CTGGGCATCG AGATCGGGGA GGTGACCTCC AGTCGCGTCT ATAACAGCAC CACAGTGCCA 
GTTGGCAACA GTGGCGGATG GCGGAATGCC CAGTCCAGTG TTACGCCACC ACCCATACCC 
ACACCGCCAC AGGTGAACTA TACCAATGGG AATGAGCCCA GCAAGCAGCC GCCACTGGAA 
CCTGTCGTGG AGGAGGAGGT GCACTTCGAC ACCGATGCCC TCAATAATCT GCCAGCTCGC 
GAGCCTGTGG ACATGGAGTT CAAGGAGCTG TCCCTCACCG TCAAATTGGG CTTCAATCGA 
GGATCCAAGG AAATCTTGCA CAATGTGTGC GGAAAGTTCC CAGGCAGCCA GCTGATTGCC 
ATCATGGGAC CTTCGGGTGC AGGAAAGTCC ACTTTGCTAG ATGCCCTTTC AGGTTTCAAG 
ACGACCGGAG TGGATGGCTC CATCTTGCTC AATGGTCGGC GACGTGATTT GCCTTCCTTC 
CGTCGGATGT CCTGCTACAT CACCCAGGAT GATCGTCTGC AGCCGCTGCT GACGGTAAAC 
GAGAATATGC ATATAGCCGC CGATCTTAAA CTGGGTCAGA CAGTCAGCTA CGAGGAGAAG 
GAGAGCAGGA TCGAGGACAT TCTGCTGCTC CTTGGTCTGT ACAATCACGA TCAGACCCTT 
ACAATGCGCC TGTCCGGTGG TCAGAAGAAG AGGCTATCCA TTGCCATGGA GTTGATCAAT 
AATCCCACTG TGATGTTCCT GGACGAGCCC ACAACTGGCT TGGACAGTAG CTCGTGCACA 
AAGGTCCTGG AGCTGCTCAA GAAGTTGACC AGCCAAGGAC GCACCATTAT TTGCACCATC 
CATCAGCCGA CGGCCAAGTT GTTCCAGATC TTCGATCAGG TCTACGTCCT GTCCGCTGGC 
AACTGCGTCT ACCAGGGCAG CACCCAGAAA TTGGTGCCGT TCCTGCAGTC GGTGGATCTG 
CCCTGTCCGA TGTACCACAA TCCAGCGGAT TACATTATCG AGCTCGCTTG CGGAGAGTAT 
GGATACGACA AGGTCGACAC CCTTAAGCTC GCCACTGAGA ACGGAAGCTG CCTGACTTGG 
TTCCATAATC CCAGTGCGGT GTTGCGCGCT GAGGTCTTGA TGAGAAAGTA TCCCATACCA 
AAGAAAACCA AAAGCCGATC CCTGGAGGAC ACCAGCTACT CAAACCAGTG CTCTGTGCTT 
TTGCGACGAG GTTTCATCAA AGCCAAACGA GACACGACCA TGACGCACTT GAGGATCGGA 
GTCAACATAG CAGTGGCCGC TCTGTTCGGA GCTATGTACG ATCACACGGG ACGTGAAGGA 
TCACGAGTGC TCGACAACTA CAATCTTCTG TTTGCCATAC TGATGCACCA TTCCATGACC 
ACGATGATGT TGACTGTTCT GACTTTCCCC ATGGACATCT CCATATTGAT CAAGGAGCAC 
TTCAATCGCT GGTACTCACT CAAAGCCTAC TACACTGCCA TGACACTCGT GGACCTGCCC 
ATATCTATCA TAAGTTGTTT CTTCTTCACT GTCATCGTTT ACCTTTGGAG TTATCAGCCG 
ATGGAGTGGA TCAGATTCTT TATGTTCTTC TCCATTAGTT TACTGACCGT CTTTGTGGGA 
CATAGTTTTG GTTTGATGAT TGGTGCCTGG TTCGATGTGG TGAATGGAAC GTTCTTGGCC 
CCAGTGCTAA CGATTCCCAT GATGATGTTC GCCGGCTTTG GAGTGACCCT GCGTGATCTG 
CCAAGCTACT TAAGGTGGGG CAGTCACATA TCATACTTAA GATATGGACT TGAGGGCTTC 
ATATCAGCCA TTTATGGCTT GGATCGAGGT ACCTTGGCCT GCGAGGAGGC GCCGTACTGC 
CATTACAGGT ATCCAAAGAA ATTCTTAGAG GAAATCACCA TGAGGGGTGA TCAGTTCTGG 
AACGATGTGA TCGCGCTGGG AGTCATGATC CTGGTTTTCC GATTTGTGTC CTACGTGGTG 
CTGAAGGCCA AGATCAAGTC GATCCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285786.1
CDS	410..2479
Translation	MATPREQFLFGSDNGVAGLGLGIEIGEVTSSRVYNSTTVPVGNSGGWRNAQSSVTPPPIPTPPQVNYTNGNEPSKQPPLEPVVEEEVHFDTDALNNLPAREPVDMEFKELSLTVKLGFNRGSKEILHNVCGKFPGSQLIAIMGPSGAGKSTLLDALSGFKTTGVDGSILLNGRRRDLPSFRRMSCYITQDDRLQPLLTVNENMHIAADLKLGQTVSYEEKESRIEDILLLLGLYNHDQTLTMRLSGGQKKRLSIAMELINNPTVMFLDEPTTGLDSSSCTKVLELLKKLTSQGRTIICTIHQPTAKLFQIFDQVYVLSAGNCVYQGSTQKLVPFLQSVDLPCPMYHNPADYIIELACGEYGYDKVDTLKLATENGSCLTWFHNPSAVLRAEVLMRKYPIPKKTKSRSLEDTSYSNQCSVLLRRGFIKAKRDTTMTHLRIGVNIAVAALFGAMYDHTGREGSRVLDNYNLLFAILMHHSMTTMMLTVLTFPMDISILIKEHFNRWYSLKAYYTAMTLVDLPISIISCFFFTVIVYLWSYQPMEWIRFFMFFSISLLTVFVGHSFGLMIGAWFDVVNGTFLAPVLTIPMMMFAGFGVTLRDLPSYLRWGSHISYLRYGLEGFISAIYGLDRGTLACEEAPYCHYRYPKKFLEEITMRGDQFWNDVIALGVMILVFRFVSYVVLKAKIKSIR

Target ORF information:

RefSeq Version	NM_001298857.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG5853), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298857.1

ORF Insert Sequence:

ATGGCGACAC CGCGCGAGCA GTTCTTGTTC GGCAGCGACA ACGGAGTGGC CGGCCTCGGT 
CTGGGCATCG AGATCGGGGA GGTGACCTCC AGTCGCGTCT ATAACAGCAC CACAGTGCCA 
GTTGGCAACA GTGGCGGATG GCGGAATGCC CAGTCCAGTG TTACGCCACC ACCCATACCC 
ACACCGCCAC AGGTGAACTA TACCAATGGG AATGAGCCCA GCAAGCAGCC GCCACTGGAA 
CCTGTCGTGG AGGAGGAGGT GCACTTCGAC ACCGATGCCC TCAATAATCT GCCAGCTCGC 
GAGCCTGTGG ACATGGAGTT CAAGGAGCTG TCCCTCACCG TCAAATTGGG CTTCAATCGA 
GGATCCAAGG AAATCTTGCA CAATGTGTGC GGAAAGTTCC CAGGCAGCCA GCTGATTGCC 
ATCATGGGAC CTTCGGGTGC AGGAAAGTCC ACTTTGCTAG ATGCCCTTTC AGGTTTCAAG 
ACGACCGGAG TGGATGGCTC CATCTTGCTC AATGGTCGGC GACGTGATTT GCCTTCCTTC 
CGTCGGATGT CCTGCTACAT CACCCAGGAT GATCGTCTGC AGCCGCTGCT GACGGTAAAC 
GAGAATATGC ATATAGCCGC CGATCTTAAA CTGGGTCAGA CAGTCAGCTA CGAGGAGAAG 
GAGAGCAGGA TCGAGGACAT TCTGCTGCTC CTTGGTCTGT ACAATCACGA TCAGACCCTT 
ACAATGCGCC TGTCCGGTGG TCAGAAGAAG AGGCTATCCA TTGCCATGGA GTTGATCAAT 
AATCCCACTG TGATGTTCCT GGACGAGCCC ACAACTGGCT TGGACAGTAG CTCGTGCACA 
AAGGTCCTGG AGCTGCTCAA GAAGTTGACC AGCCAAGGAC GCACCATTAT TTGCACCATC 
CATCAGCCGA CGGCCAAGTT GTTCCAGATC TTCGATCAGG TCTACGTCCT GTCCGCTGGC 
AACTGCGTCT ACCAGGGCAG CACCCAGAAA TTGGTGCCGT TCCTGCAGTC GGTGGATCTG 
CCCTGTCCGA TGTACCACAA TCCAGCGGAT TACATTATCG AGCTCGCTTG CGGAGAGTAT 
GGATACGACA AGGTCGACAC CCTTAAGCTC GCCACTGAGA ACGGAAGCTG CCTGACTTGG 
TTCCATAATC CCAGTGCGGT GTTGCGCGCT GAGGTCTTGA TGAGAAAGTA TCCCATACCA 
AAGAAAACCA AAAGCCGATC CCTGGAGGAC ACCAGCTACT CAAACCAGTG CTCTGTGCTT 
TTGCGACGAG GTTTCATCAA AGCCAAACGA GACACGACCA TGACGCACTT GAGGATCGGA 
GTCAACATAG CAGTGGCCGC TCTGTTCGGA GCTATGTACG ATCACACGGG ACGTGAAGGA 
TCACGAGTGC TCGACAACTA CAATCTTCTG TTTGCCATAC TGATGCACCA TTCCATGACC 
ACGATGATGT TGACTGTTCT GACTTTCCCC ATGGACATCT CCATATTGAT CAAGGAGCAC 
TTCAATCGCT GGTACTCACT CAAAGCCTAC TACACTGCCA TGACACTCGT GGACCTGCCC 
ATATCTATCA TAAGTTGTTT CTTCTTCACT GTCATCGTTT ACCTTTGGAG TTATCAGCCG 
ATGGAGTGGA TCAGATTCTT TATGTTCTTC TCCATTAGTT TACTGACCGT CTTTGTGGGA 
CATAGTTTTG GTTTGATGAT TGGTGCCTGG TTCGATGTGG TGAATGGAAC GTTCTTGGCC 
CCAGTGCTAA CGATTCCCAT GATGATGTTC GCCGGCTTTG GAGTGACCCT GCGTGATCTG 
CCAAGCTACT TAAGGTGGGG CAGTCACATA TCATACTTAA GATATGGACT TGAGGGCTTC 
ATATCAGCCA TTTATGGCTT GGATCGAGGT ACCTTGGCCT GCGAGGAGGC GCCGTACTGC 
CATTACAGGT ATCCAAAGAA ATTCTTAGAG GAAATCACCA TGAGGGGTGA TCAGTTCTGG 
AACGATGTGA TCGCGCTGGG AGTCATGATC CTGGTTTTCC GATTTGTGTC CTACGTGGTG 
CTGAAGGCCA AGATCAAGTC GATCCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG5853 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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