numb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	numb
Entrez Gene ID	34263
Full Name	CG3779 gene product from transcript CG3779-RB
Synonyms	CG3779,Dmel\CG3779,N7-1,Nb,Numb,d-numb,dNumb,l(2)03235,l(2)3235,l(2)s2201,nb,numbs
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 30B3-30B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273362.1	NP_001260291.1	numb, isoform D
NM_078799.3	NP_523523.2	numb, isoform A
NM_164855.2	NP_723460.1	numb, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	numb	NP_001260291.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010167	XP_319339.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors find that Numb is regulated in part through its asymmetric and symmetric distribution during stem cell divisions; however, its de novo synthesis is also required during the differentiation of the enteroendocrine cells.
Title: Intrinsic regulation of enteroendocrine fate by Numb.

Inscuteable maintains type I neuroblast lineage identity via Numb/Notch signaling in the Drosophila larval brain
Title: Inscuteable maintains type I neuroblast lineage identity via Numb/Notch signaling in the Drosophila larval brain.

Data suggest that Numb controls the balance between Notch receptor recycling and receptor targeting to late endosomes to regulate signaling output after asymmetric cell division in Drosophila neural progenitors.
Title: Numb regulates the balance between Notch recycling and late-endosome targeting in Drosophila neural progenitor cells.

Spdo is sorted toward late endosomes in a Numb-dependent manner.
Title: A fluorescent tagging approach in Drosophila reveals late endosomal trafficking of Notch and Sanpodo.

Data show regulation of centrosome movements by numb and the collapsin response mediator protein during Drosophila sensory progenitor asymmetric division.
Title: Regulation of centrosome movements by numb and the collapsin response mediator protein during Drosophila sensory progenitor asymmetric division.

The following numb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the numb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14195	NM_164855.2 Latest version!	Drosophila melanogaster numb, transcript variant B (numb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14196	NM_078799.3 Latest version!	Drosophila melanogaster numb, transcript variant A (numb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14196	NM_001273362.1 Latest version!	Drosophila melanogaster numb, transcript variant D (numb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

numb ( NM_164855.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723460.1 Drosophila melanogaster numb, transcript variant B (numb), mRNA.

Price & Availability↑

CloneID	OFa14195
Clone ID Related Accession (Same CDS sequence)	NM_164855.2
Accession Version	NM_164855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	numb, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164855.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGTC TGCGTCGCTC CTTTCGCGAT TCCTTTAGGC GTCGCAAGGA TCGCGTTCCC 
GAATCCTCGA AGCCGCATCA ATGGCAGGCG GACGAGGAGG CCGTGCGATC GGCCACCTGC 
TCCTTTTCCG TCAAGTATCT GGGATGTGTC GAGGTCTTTG AATCGCGCGG CATGCAGGTC 
TGCGAGGAGG CACTCAAGGT CTTGAGGCAA TCCCGTCGTC GTCCTGTCCG CGGATTACTT 
CATGTCAGCG GAGACGGCCT GCGAGTGGTG GACGACGAGA CCAAGGGCCT GATAGTGGAC 
CAGACCATCG AGAAGGTCAG CTTCTGTGCA CCGGATCGCA ATCACGAACG CGGCTTCAGC 
TACATATGCC GGGATGGAAC CACTCGTCGC TGGATGTGTC ACGGCTTCCT GGCGTGCAAG 
GACTCCGGCG AGCGATTGTC CCATGCCGTT GGCTGTGCTT TTGCCGTTTG TCTGGAGCGA 
AAGCAGCGAC GCGACAAGGA GTGCGGCGTG ACAATGACCT TCGACACGAA GAACTCGACC 
TTCACGCGGA CCGGATCCTT CCGTCAGCAG ACGTTGACGG AGCGTCTGGC AATGGCCACA 
GTGGGCACAA ATGAGCGGAG CGTAGATGGG CCGGGATCAG CAATGCCGGG TCCTCCGGCG 
GCCACCGTCA AGCCTTTCAA TCCCTTCGCC ATCGAACGGC CCCATGCCAC GCCCAACATG 
CTGGAGCGTC AGAGTTCGTT CCGTTTGAGC ACGATTGGCA GTCAGTCGCC GTTCAAGCGC 
CAGATGTCAC TGCGCATCAA CGATCTTCCG TCCAATGCCG ATCGTCAGAG GGCCTTCCTG 
ACCGCCGCCG CTGGCAATCC AATGCAGACG CCACTGCGAA GTGTTTCTCC CATTGCCGAG 
GTCTCGCCGG CCAAGTCTGC TGGAGCGGAT CCGTTGTCAG CTGCCGCTGT AGCCGCCGAT 
TCTGTGAGCC AACTGTGTCA GGAGCTCAGC CAGGGTCTCT CGCTCTTGAC CCAGACAGAC 
GCCCTATTAG CCGCCGGCGA GGATCTAAAC TTCAACAACA ATCGCAGCAT TAATCAGAAC 
ATCATTGCCG CCGAGAAGCA GGTGCAACAT GTACACTCAT ATGCCCCGCC GACGGCACAA 
GTGACGCCTC GAGTGGCCAG CACCACGCCC ACATATCAGA CGCTCCATTC GCAGTCGCCC 
TCGCGATCGG AGCAGAGCAT CGAAACCTCG ACGGAACTGC CCAATGCCGA GCAATGGCTG 
GGTCATGTGG TGCGCAGTAC GTCTCCGGCA GCACCGAAGC GGCCCACTTA TTTGGCCAAC 
GTTGGCCGAG CTCAGACACT GGCCAGTGGC ACTGGCGCTG CAGTTGGAGG CGGTGGCCCA 
GACGATCCAT TTGACGCCGA GTGGGTGGCC AATGTGGCAG CGGCCAAGCA ATTGTCGCCA 
GACTTGCCAA TCCCGAGCAC TGCGCGCTCG CCTCTGGCCA GGCACAGCAC CAATCCGTTC 
ATCTCACCGC CCAAGGCGCC GGCGCAGTCA TTCCAGGTGC AGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723460.1
CDS	564..2111
Translation	MDRLRRSFRDSFRRRKDRVPESSKPHQWQADEEAVRSATCSFSVKYLGCVEVFESRGMQVCEEALKVLRQSRRRPVRGLLHVSGDGLRVVDDETKGLIVDQTIEKVSFCAPDRNHERGFSYICRDGTTRRWMCHGFLACKDSGERLSHAVGCAFAVCLERKQRRDKECGVTMTFDTKNSTFTRTGSFRQQTLTERLAMATVGTNERSVDGPGSAMPGPPAATVKPFNPFAIERPHATPNMLERQSSFRLSTIGSQSPFKRQMSLRINDLPSNADRQRAFLTAAAGNPMQTPLRSVSPIAEVSPAKSAGADPLSAAAVAADSVSQLCQELSQGLSLLTQTDALLAAGEDLNFNNNRSINQNIIAAEKQVQHVHSYAPPTAQVTPRVASTTPTYQTLHSQSPSRSEQSIETSTELPNAEQWLGHVVRSTSPAAPKRPTYLANVGRAQTLASGTGAAVGGGGPDDPFDAEWVANVAAAKQLSPDLPIPSTARSPLARHSTNPFISPPKAPAQSFQVQL

Target ORF information:

RefSeq Version	NM_164855.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster numb, transcript variant B (numb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164855.2

ORF Insert Sequence:

ATGGATCGTC TGCGTCGCTC CTTTCGCGAT TCCTTTAGGC GTCGCAAGGA TCGCGTTCCC 
GAATCCTCGA AGCCGCATCA ATGGCAGGCG GACGAGGAGG CCGTGCGATC GGCCACCTGC 
TCCTTTTCCG TCAAGTATCT GGGATGTGTC GAGGTCTTTG AATCGCGCGG CATGCAGGTC 
TGCGAGGAGG CACTCAAGGT CTTGAGGCAA TCCCGTCGTC GTCCTGTCCG CGGATTACTT 
CATGTCAGCG GAGACGGCCT GCGAGTGGTG GACGACGAGA CCAAGGGCCT GATAGTGGAC 
CAGACCATCG AGAAGGTCAG CTTCTGTGCA CCGGATCGCA ATCACGAACG CGGCTTCAGC 
TACATATGCC GGGATGGAAC CACTCGTCGC TGGATGTGTC ACGGCTTCCT GGCGTGCAAG 
GACTCCGGCG AGCGATTGTC CCATGCCGTT GGCTGTGCTT TTGCCGTTTG TCTGGAGCGA 
AAGCAGCGAC GCGACAAGGA GTGCGGCGTG ACAATGACCT TCGACACGAA GAACTCGACC 
TTCACGCGGA CCGGATCCTT CCGTCAGCAG ACGTTGACGG AGCGTCTGGC AATGGCCACA 
GTGGGCACAA ATGAGCGGAG CGTAGATGGG CCGGGATCAG CAATGCCGGG TCCTCCGGCG 
GCCACCGTCA AGCCTTTCAA TCCCTTCGCC ATCGAACGGC CCCATGCCAC GCCCAACATG 
CTGGAGCGTC AGAGTTCGTT CCGTTTGAGC ACGATTGGCA GTCAGTCGCC GTTCAAGCGC 
CAGATGTCAC TGCGCATCAA CGATCTTCCG TCCAATGCCG ATCGTCAGAG GGCCTTCCTG 
ACCGCCGCCG CTGGCAATCC AATGCAGACG CCACTGCGAA GTGTTTCTCC CATTGCCGAG 
GTCTCGCCGG CCAAGTCTGC TGGAGCGGAT CCGTTGTCAG CTGCCGCTGT AGCCGCCGAT 
TCTGTGAGCC AACTGTGTCA GGAGCTCAGC CAGGGTCTCT CGCTCTTGAC CCAGACAGAC 
GCCCTATTAG CCGCCGGCGA GGATCTAAAC TTCAACAACA ATCGCAGCAT TAATCAGAAC 
ATCATTGCCG CCGAGAAGCA GGTGCAACAT GTACACTCAT ATGCCCCGCC GACGGCACAA 
GTGACGCCTC GAGTGGCCAG CACCACGCCC ACATATCAGA CGCTCCATTC GCAGTCGCCC 
TCGCGATCGG AGCAGAGCAT CGAAACCTCG ACGGAACTGC CCAATGCCGA GCAATGGCTG 
GGTCATGTGG TGCGCAGTAC GTCTCCGGCA GCACCGAAGC GGCCCACTTA TTTGGCCAAC 
GTTGGCCGAG CTCAGACACT GGCCAGTGGC ACTGGCGCTG CAGTTGGAGG CGGTGGCCCA 
GACGATCCAT TTGACGCCGA GTGGGTGGCC AATGTGGCAG CGGCCAAGCA ATTGTCGCCA 
GACTTGCCAA TCCCGAGCAC TGCGCGCTCG CCTCTGGCCA GGCACAGCAC CAATCCGTTC 
ATCTCACCGC CCAAGGCGCC GGCGCAGTCA TTCCAGGTGC AGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

numb ( NM_001273362.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260291.1 Drosophila melanogaster numb, transcript variant D (numb), mRNA.

Price & Availability↑

CloneID	OFa14196
Clone ID Related Accession (Same CDS sequence)	NM_001273362.1 , NM_078799.3
Accession Version	NM_001273362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	numb, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAACT CCTCGTCACA CACGCACGAA CCACTCGAGC GCGGCTTCAC ACGCGGAAAA 
TTCGGTGATG TTAAAAATGG GAAATCCGCT TCCTTCCGGT TTAGCAAGAA ATCGCCGAAG 
AAAATGGATC GTCTGCGTCG CTCCTTTCGC GATTCCTTTA GGCGTCGCAA GGATCGCGTT 
CCCGAATCCT CGAAGCCGCA TCAATGGCAG GCGGACGAGG AGGCCGTGCG ATCGGCCACC 
TGCTCCTTTT CCGTCAAGTA TCTGGGATGT GTCGAGGTCT TTGAATCGCG CGGCATGCAG 
GTCTGCGAGG AGGCACTCAA GGTCTTGAGG CAATCCCGTC GTCGTCCTGT CCGCGGATTA 
CTTCATGTCA GCGGAGACGG CCTGCGAGTG GTGGACGACG AGACCAAGGG CCTGATAGTG 
GACCAGACCA TCGAGAAGGT CAGCTTCTGT GCACCGGATC GCAATCACGA ACGCGGCTTC 
AGCTACATAT GCCGGGATGG AACCACTCGT CGCTGGATGT GTCACGGCTT CCTGGCGTGC 
AAGGACTCCG GCGAGCGATT GTCCCATGCC GTTGGCTGTG CTTTTGCCGT TTGTCTGGAG 
CGAAAGCAGC GACGCGACAA GGAGTGCGGC GTGACAATGA CCTTCGACAC GAAGAACTCG 
ACCTTCACGC GGACCGGATC CTTCCGTCAG CAGACGTTGA CGGAGCGTCT GGCAATGGCC 
ACAGTGGGCA CAAATGAGCG GAGCGTAGAT GGGCCGGGAT CAGCAATGCC GGGTCCTCCG 
GCGGCCACCG TCAAGCCTTT CAATCCCTTC GCCATCGAAC GGCCCCATGC CACGCCCAAC 
ATGCTGGAGC GTCAGAGTTC GTTCCGTTTG AGCACGATTG GCAGTCAGTC GCCGTTCAAG 
CGCCAGATGT CACTGCGCAT CAACGATCTT CCGTCCAATG CCGATCGTCA GAGGGCCTTC 
CTGACCGCCG CCGCTGGCAA TCCAATGCAG ACGCCACTGC GAAGTGTTTC TCCCATTGCC 
GAGGTCTCGC CGGCCAAGTC TGCTGGAGCG GATCCGTTGT CAGCTGCCGC TGTAGCCGCC 
GATTCTGTGA GCCAACTGTG TCAGGAGCTC AGCCAGGGTC TCTCGCTCTT GACCCAGACA 
GACGCCCTAT TAGCCGCCGG CGAGGATCTA AACTTCAACA ACAATCGCAG CATTAATCAG 
AACATCATTG CCGCCGAGAA GCAGGTGCAA CATGTACACT CATATGCCCC GCCGACGGCA 
CAAGTGACGC CTCGAGTGGC CAGCACCACG CCCACATATC AGACGCTCCA TTCGCAGTCG 
CCCTCGCGAT CGGAGCAGAG CATCGAAACC TCGACGGAAC TGCCCAATGC CGAGCAATGG 
CTGGGTCATG TGGTGCGCAG TACGTCTCCG GCAGCACCGA AGCGGCCCAC TTATTTGGCC 
AACGTTGGCC GAGCTCAGAC ACTGGCCAGT GGCACTGGCG CTGCAGTTGG AGGCGGTGGC 
CCAGACGATC CATTTGACGC CGAGTGGGTG GCCAATGTGG CAGCGGCCAA GCAATTGTCG 
CCAGACTTGC CAATCCCGAG CACTGCGCGC TCGCCTCTGG CCAGGCACAG CACCAATCCG 
TTCATCTCAC CGCCCAAGGC GCCGGCGCAG TCATTCCAGG TGCAGCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260291.1
CDS	819..2489
Translation	MGNSSSHTHEPLERGFTRGKFGDVKNGKSASFRFSKKSPKKMDRLRRSFRDSFRRRKDRVPESSKPHQWQADEEAVRSATCSFSVKYLGCVEVFESRGMQVCEEALKVLRQSRRRPVRGLLHVSGDGLRVVDDETKGLIVDQTIEKVSFCAPDRNHERGFSYICRDGTTRRWMCHGFLACKDSGERLSHAVGCAFAVCLERKQRRDKECGVTMTFDTKNSTFTRTGSFRQQTLTERLAMATVGTNERSVDGPGSAMPGPPAATVKPFNPFAIERPHATPNMLERQSSFRLSTIGSQSPFKRQMSLRINDLPSNADRQRAFLTAAAGNPMQTPLRSVSPIAEVSPAKSAGADPLSAAAVAADSVSQLCQELSQGLSLLTQTDALLAAGEDLNFNNNRSINQNIIAAEKQVQHVHSYAPPTAQVTPRVASTTPTYQTLHSQSPSRSEQSIETSTELPNAEQWLGHVVRSTSPAAPKRPTYLANVGRAQTLASGTGAAVGGGGPDDPFDAEWVANVAAAKQLSPDLPIPSTARSPLARHSTNPFISPPKAPAQSFQVQL

Target ORF information:

RefSeq Version	NM_001273362.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster numb, transcript variant D (numb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273362.1

ORF Insert Sequence:

ATGGGAAACT CCTCGTCACA CACGCACGAA CCACTCGAGC GCGGCTTCAC ACGCGGAAAA 
TTCGGTGATG TTAAAAATGG GAAATCCGCT TCCTTCCGGT TTAGCAAGAA ATCGCCGAAG 
AAAATGGATC GTCTGCGTCG CTCCTTTCGC GATTCCTTTA GGCGTCGCAA GGATCGCGTT 
CCCGAATCCT CGAAGCCGCA TCAATGGCAG GCGGACGAGG AGGCCGTGCG ATCGGCCACC 
TGCTCCTTTT CCGTCAAGTA TCTGGGATGT GTCGAGGTCT TTGAATCGCG CGGCATGCAG 
GTCTGCGAGG AGGCACTCAA GGTCTTGAGG CAATCCCGTC GTCGTCCTGT CCGCGGATTA 
CTTCATGTCA GCGGAGACGG CCTGCGAGTG GTGGACGACG AGACCAAGGG CCTGATAGTG 
GACCAGACCA TCGAGAAGGT CAGCTTCTGT GCACCGGATC GCAATCACGA ACGCGGCTTC 
AGCTACATAT GCCGGGATGG AACCACTCGT CGCTGGATGT GTCACGGCTT CCTGGCGTGC 
AAGGACTCCG GCGAGCGATT GTCCCATGCC GTTGGCTGTG CTTTTGCCGT TTGTCTGGAG 
CGAAAGCAGC GACGCGACAA GGAGTGCGGC GTGACAATGA CCTTCGACAC GAAGAACTCG 
ACCTTCACGC GGACCGGATC CTTCCGTCAG CAGACGTTGA CGGAGCGTCT GGCAATGGCC 
ACAGTGGGCA CAAATGAGCG GAGCGTAGAT GGGCCGGGAT CAGCAATGCC GGGTCCTCCG 
GCGGCCACCG TCAAGCCTTT CAATCCCTTC GCCATCGAAC GGCCCCATGC CACGCCCAAC 
ATGCTGGAGC GTCAGAGTTC GTTCCGTTTG AGCACGATTG GCAGTCAGTC GCCGTTCAAG 
CGCCAGATGT CACTGCGCAT CAACGATCTT CCGTCCAATG CCGATCGTCA GAGGGCCTTC 
CTGACCGCCG CCGCTGGCAA TCCAATGCAG ACGCCACTGC GAAGTGTTTC TCCCATTGCC 
GAGGTCTCGC CGGCCAAGTC TGCTGGAGCG GATCCGTTGT CAGCTGCCGC TGTAGCCGCC 
GATTCTGTGA GCCAACTGTG TCAGGAGCTC AGCCAGGGTC TCTCGCTCTT GACCCAGACA 
GACGCCCTAT TAGCCGCCGG CGAGGATCTA AACTTCAACA ACAATCGCAG CATTAATCAG 
AACATCATTG CCGCCGAGAA GCAGGTGCAA CATGTACACT CATATGCCCC GCCGACGGCA 
CAAGTGACGC CTCGAGTGGC CAGCACCACG CCCACATATC AGACGCTCCA TTCGCAGTCG 
CCCTCGCGAT CGGAGCAGAG CATCGAAACC TCGACGGAAC TGCCCAATGC CGAGCAATGG 
CTGGGTCATG TGGTGCGCAG TACGTCTCCG GCAGCACCGA AGCGGCCCAC TTATTTGGCC 
AACGTTGGCC GAGCTCAGAC ACTGGCCAGT GGCACTGGCG CTGCAGTTGG AGGCGGTGGC 
CCAGACGATC CATTTGACGC CGAGTGGGTG GCCAATGTGG CAGCGGCCAA GCAATTGTCG 
CCAGACTTGC CAATCCCGAG CACTGCGCGC TCGCCTCTGG CCAGGCACAG CACCAATCCG 
TTCATCTCAC CGCCCAAGGC GCCGGCGCAG TCATTCCAGG TGCAGCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

numb ( NM_078799.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523523.2 Drosophila melanogaster numb, transcript variant A (numb), mRNA.

Price & Availability↑

CloneID	OFa14196
Clone ID Related Accession (Same CDS sequence)	NM_001273362.1 , NM_078799.3
Accession Version	NM_078799.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	numb, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078799.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAACT CCTCGTCACA CACGCACGAA CCACTCGAGC GCGGCTTCAC ACGCGGAAAA 
TTCGGTGATG TTAAAAATGG GAAATCCGCT TCCTTCCGGT TTAGCAAGAA ATCGCCGAAG 
AAAATGGATC GTCTGCGTCG CTCCTTTCGC GATTCCTTTA GGCGTCGCAA GGATCGCGTT 
CCCGAATCCT CGAAGCCGCA TCAATGGCAG GCGGACGAGG AGGCCGTGCG ATCGGCCACC 
TGCTCCTTTT CCGTCAAGTA TCTGGGATGT GTCGAGGTCT TTGAATCGCG CGGCATGCAG 
GTCTGCGAGG AGGCACTCAA GGTCTTGAGG CAATCCCGTC GTCGTCCTGT CCGCGGATTA 
CTTCATGTCA GCGGAGACGG CCTGCGAGTG GTGGACGACG AGACCAAGGG CCTGATAGTG 
GACCAGACCA TCGAGAAGGT CAGCTTCTGT GCACCGGATC GCAATCACGA ACGCGGCTTC 
AGCTACATAT GCCGGGATGG AACCACTCGT CGCTGGATGT GTCACGGCTT CCTGGCGTGC 
AAGGACTCCG GCGAGCGATT GTCCCATGCC GTTGGCTGTG CTTTTGCCGT TTGTCTGGAG 
CGAAAGCAGC GACGCGACAA GGAGTGCGGC GTGACAATGA CCTTCGACAC GAAGAACTCG 
ACCTTCACGC GGACCGGATC CTTCCGTCAG CAGACGTTGA CGGAGCGTCT GGCAATGGCC 
ACAGTGGGCA CAAATGAGCG GAGCGTAGAT GGGCCGGGAT CAGCAATGCC GGGTCCTCCG 
GCGGCCACCG TCAAGCCTTT CAATCCCTTC GCCATCGAAC GGCCCCATGC CACGCCCAAC 
ATGCTGGAGC GTCAGAGTTC GTTCCGTTTG AGCACGATTG GCAGTCAGTC GCCGTTCAAG 
CGCCAGATGT CACTGCGCAT CAACGATCTT CCGTCCAATG CCGATCGTCA GAGGGCCTTC 
CTGACCGCCG CCGCTGGCAA TCCAATGCAG ACGCCACTGC GAAGTGTTTC TCCCATTGCC 
GAGGTCTCGC CGGCCAAGTC TGCTGGAGCG GATCCGTTGT CAGCTGCCGC TGTAGCCGCC 
GATTCTGTGA GCCAACTGTG TCAGGAGCTC AGCCAGGGTC TCTCGCTCTT GACCCAGACA 
GACGCCCTAT TAGCCGCCGG CGAGGATCTA AACTTCAACA ACAATCGCAG CATTAATCAG 
AACATCATTG CCGCCGAGAA GCAGGTGCAA CATGTACACT CATATGCCCC GCCGACGGCA 
CAAGTGACGC CTCGAGTGGC CAGCACCACG CCCACATATC AGACGCTCCA TTCGCAGTCG 
CCCTCGCGAT CGGAGCAGAG CATCGAAACC TCGACGGAAC TGCCCAATGC CGAGCAATGG 
CTGGGTCATG TGGTGCGCAG TACGTCTCCG GCAGCACCGA AGCGGCCCAC TTATTTGGCC 
AACGTTGGCC GAGCTCAGAC ACTGGCCAGT GGCACTGGCG CTGCAGTTGG AGGCGGTGGC 
CCAGACGATC CATTTGACGC CGAGTGGGTG GCCAATGTGG CAGCGGCCAA GCAATTGTCG 
CCAGACTTGC CAATCCCGAG CACTGCGCGC TCGCCTCTGG CCAGGCACAG CACCAATCCG 
TTCATCTCAC CGCCCAAGGC GCCGGCGCAG TCATTCCAGG TGCAGCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523523.2
CDS	794..2464
Translation	MGNSSSHTHEPLERGFTRGKFGDVKNGKSASFRFSKKSPKKMDRLRRSFRDSFRRRKDRVPESSKPHQWQADEEAVRSATCSFSVKYLGCVEVFESRGMQVCEEALKVLRQSRRRPVRGLLHVSGDGLRVVDDETKGLIVDQTIEKVSFCAPDRNHERGFSYICRDGTTRRWMCHGFLACKDSGERLSHAVGCAFAVCLERKQRRDKECGVTMTFDTKNSTFTRTGSFRQQTLTERLAMATVGTNERSVDGPGSAMPGPPAATVKPFNPFAIERPHATPNMLERQSSFRLSTIGSQSPFKRQMSLRINDLPSNADRQRAFLTAAAGNPMQTPLRSVSPIAEVSPAKSAGADPLSAAAVAADSVSQLCQELSQGLSLLTQTDALLAAGEDLNFNNNRSINQNIIAAEKQVQHVHSYAPPTAQVTPRVASTTPTYQTLHSQSPSRSEQSIETSTELPNAEQWLGHVVRSTSPAAPKRPTYLANVGRAQTLASGTGAAVGGGGPDDPFDAEWVANVAAAKQLSPDLPIPSTARSPLARHSTNPFISPPKAPAQSFQVQL

Target ORF information:

RefSeq Version	NM_078799.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster numb, transcript variant A (numb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078799.3

ORF Insert Sequence:

ATGGGAAACT CCTCGTCACA CACGCACGAA CCACTCGAGC GCGGCTTCAC ACGCGGAAAA 
TTCGGTGATG TTAAAAATGG GAAATCCGCT TCCTTCCGGT TTAGCAAGAA ATCGCCGAAG 
AAAATGGATC GTCTGCGTCG CTCCTTTCGC GATTCCTTTA GGCGTCGCAA GGATCGCGTT 
CCCGAATCCT CGAAGCCGCA TCAATGGCAG GCGGACGAGG AGGCCGTGCG ATCGGCCACC 
TGCTCCTTTT CCGTCAAGTA TCTGGGATGT GTCGAGGTCT TTGAATCGCG CGGCATGCAG 
GTCTGCGAGG AGGCACTCAA GGTCTTGAGG CAATCCCGTC GTCGTCCTGT CCGCGGATTA 
CTTCATGTCA GCGGAGACGG CCTGCGAGTG GTGGACGACG AGACCAAGGG CCTGATAGTG 
GACCAGACCA TCGAGAAGGT CAGCTTCTGT GCACCGGATC GCAATCACGA ACGCGGCTTC 
AGCTACATAT GCCGGGATGG AACCACTCGT CGCTGGATGT GTCACGGCTT CCTGGCGTGC 
AAGGACTCCG GCGAGCGATT GTCCCATGCC GTTGGCTGTG CTTTTGCCGT TTGTCTGGAG 
CGAAAGCAGC GACGCGACAA GGAGTGCGGC GTGACAATGA CCTTCGACAC GAAGAACTCG 
ACCTTCACGC GGACCGGATC CTTCCGTCAG CAGACGTTGA CGGAGCGTCT GGCAATGGCC 
ACAGTGGGCA CAAATGAGCG GAGCGTAGAT GGGCCGGGAT CAGCAATGCC GGGTCCTCCG 
GCGGCCACCG TCAAGCCTTT CAATCCCTTC GCCATCGAAC GGCCCCATGC CACGCCCAAC 
ATGCTGGAGC GTCAGAGTTC GTTCCGTTTG AGCACGATTG GCAGTCAGTC GCCGTTCAAG 
CGCCAGATGT CACTGCGCAT CAACGATCTT CCGTCCAATG CCGATCGTCA GAGGGCCTTC 
CTGACCGCCG CCGCTGGCAA TCCAATGCAG ACGCCACTGC GAAGTGTTTC TCCCATTGCC 
GAGGTCTCGC CGGCCAAGTC TGCTGGAGCG GATCCGTTGT CAGCTGCCGC TGTAGCCGCC 
GATTCTGTGA GCCAACTGTG TCAGGAGCTC AGCCAGGGTC TCTCGCTCTT GACCCAGACA 
GACGCCCTAT TAGCCGCCGG CGAGGATCTA AACTTCAACA ACAATCGCAG CATTAATCAG 
AACATCATTG CCGCCGAGAA GCAGGTGCAA CATGTACACT CATATGCCCC GCCGACGGCA 
CAAGTGACGC CTCGAGTGGC CAGCACCACG CCCACATATC AGACGCTCCA TTCGCAGTCG 
CCCTCGCGAT CGGAGCAGAG CATCGAAACC TCGACGGAAC TGCCCAATGC CGAGCAATGG 
CTGGGTCATG TGGTGCGCAG TACGTCTCCG GCAGCACCGA AGCGGCCCAC TTATTTGGCC 
AACGTTGGCC GAGCTCAGAC ACTGGCCAGT GGCACTGGCG CTGCAGTTGG AGGCGGTGGC 
CCAGACGATC CATTTGACGC CGAGTGGGTG GCCAATGTGG CAGCGGCCAA GCAATTGTCG 
CCAGACTTGC CAATCCCGAG CACTGCGCGC TCGCCTCTGG CCAGGCACAG CACCAATCCG 
TTCATCTCAC CGCCCAAGGC GCCGGCGCAG TCATTCCAGG TGCAGCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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numb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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