Eaat1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eaat1
Entrez Gene ID	34251
Full Name	Excitatory amino acid transporter 1
Synonyms	AF001784,CG3747,Dglt-1,Dglut-1,Dmel\CG3747,EAAT,EAAT1,dEAAT,dEAAT1,dEaat1,eaat1
General protein information	Preferred Names Excitatory amino acid transporter 1 Names CG3747 gene product from transcript CG3747-RA CG3747-PA CG3747-PB CG3747-PC CG3747-PD Eaat1-PA Eaat1-PB Eaat1-PC Eaat1-PD drosophila glutamate transporter-1 excitatory amino acid transporter 1 glutamate transporter sodium-dependent excitatory amino acid transporter 1 sodium-dependent glutamate transporter sodium-excitatory-glutamate-cotransporter
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 30A8-30A8

mRNA and Protein(s)

mRNA	Protein	Name
NM_058080.4	NP_477428.1	excitatory amino acid transporter 1, isoform A
NM_164853.3	NP_723454.1	excitatory amino acid transporter 1, isoform B
NM_164854.3	NP_723455.1	excitatory amino acid transporter 1, isoform C
NM_001201822.2	NP_001188751.1	excitatory amino acid transporter 1, isoform D

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SLC1A6	NP_001092560.1
Xenopus tropicalis (tropical clawed frog)	LOC100494506	XP_004911063.1
Homo sapiens (human)	SLC1A6	NP_005062.1
Canis lupus familiaris (dog)	SLC1A6	NP_001003137.1
Mus musculus (house mouse)	Slc1a6	NP_033226.1
Danio rerio (zebrafish)	slc1a6	NP_001103173.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009443	XP_560420.2
Drosophila melanogaster (fruit fly)	Eaat1	NP_001188751.1
Rattus norvegicus (Norway rat)	Slc1a6	NP_114454.1
Gallus gallus (chicken)	SLC1A6	XP_004948711.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A Drosophila tripartite synapse model which exhibits morphological and functional properties similar to those of mammalian synapses.
Title: A tripartite synapse model in Drosophila.

Eaat1 expression is promoted by Fringe-mediated neuron-glial communication during development and suggest that Eaat1 plays an essential role in regulating central nervous system neural circuits that control locomotion in Drosophila
Title: Drosophila glial glutamate transporter Eaat1 is regulated by fringe-mediated notch signaling and is essential for larval locomotion.

dEAAT1 is present at the adult, but surprisingly not at embryonic and larval NMJ, suggesting a physiological maturation of the junction during metamorphosis
Title: Physiological requirement for the glutamate transporter dEAAT1 at the adult Drosophila neuromuscular junction.

Terminal glial differentiation involves regulated expression of the excitatory amino acid transporters in the Drosophila embryonic CNS.
Title: Terminal glial differentiation involves regulated expression of the excitatory amino acid transporters in the Drosophila embryonic CNS.

The following Eaat1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eaat1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14186	NM_164853.3 Latest version!	Drosophila melanogaster excitatory amino acid transporter 1, transcript variant B (Eaat1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14186	NM_164854.3 Latest version!	Drosophila melanogaster excitatory amino acid transporter 1, transcript variant C (Eaat1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14186	NM_058080.4 Latest version!	Drosophila melanogaster excitatory amino acid transporter 1, transcript variant A (Eaat1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14186	NM_001201822.2 Latest version!	Drosophila melanogaster excitatory amino acid transporter 1, transcript variant D (Eaat1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eaat1 ( NM_058080.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477428.1 Drosophila melanogaster excitatory amino acid transporter 1, transcript variant A (Eaat1), mRNA.

Price & Availability↑

CloneID	OFa14186
Clone ID Related Accession (Same CDS sequence)	NM_058080.4 , NM_164853.3 , NM_164854.3 , NM_001201822.2
Accession Version	NM_058080.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	excitatory amino acid transporter 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_058080.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCGAC CCAAACAGGA TGGCGGCAAG TTCAAGGCCT TCATGCAGGA GAATGTCCTT 
ACCATGGCCA CCGTTATCGG TGTGTTTGTG GGTGGACTCA TCGGCTTCAT CATCAAAAAT 
AGCACTGGCG AGTGGTCGAA GAGAGAGATC ATGTACATAT CCTTCCCCGG CGAGATTTTC 
TTGCGAATGC TTAAATGTTT GATTGTGCCG CTTTTGGTCT CATCAATCAC CAGTGCCATT 
GGTGGACTCG ACCTGAGCAT GTCCAGCAAG ATTGCTACCA GAGCCATTAC TTACTACTTT 
GTGACCACCA TATCGGCCGT GATTCTGGGA ATATGTCTGG TGACCACACT GCGTCCCGGC 
CAGGGAGCCA AGATCGTGGA GACCCAGACG GAGAGCATTG ATAAGGCATC GAAGGTGCTC 
ACCCCAGACA CGCTTATGGA TTTGGTGCGA AACATGTTCA CGGACAACAT CATTCAGTCG 
ACCATGTTCC AGCACCGCAC TGAGATCTAT GAGAACACTA GCATTAGCCC AGCACAGCCT 
ATGGAAAACT GGGAGTTCAA GTCGGCTCAG CGCGAGGGTT CTAATGTCCT GGGTCTTGTG 
ATGTTCAGTG TTATCCTAGG TACCACCATT GGAAGAATGC GGGAGAAGGG ACAACTGCTG 
CAGGATTTCT TCACCACACT GAGCGAGGCA ATGATGACCA TCACCTCATG GGTTATTTGG 
ATTTCCCCGC TGGGTGTTGC CTTTCTGATA GCCGCCAAGA TCATTGAGAT GGAATCGATA 
GCAGCAACGA TTCAGTCATT AGGATGGTAT TTCATAACGG TCATGATAGG TCTATTCCTT 
CACGGTTTTG GTACGATTGC GGTGATCTTC TTCCTGGGCA CCCGACGTCT CCCGTACCGC 
TATATTGCCA AGCTTAGTCA GGTCCTGGCA ACTGCATTTG GAACAGGTTC CAGCTCGGCC 
ACCATGCCGC TGACCATCAA GTGTTTGGAC AACATGGGCA TCGATCCGCG GGTTACTCGA 
TTTGTCATTC CTGTTGGTGC CACAATTAAC ATGGACGGAA CGGCTCTCTA TGAGGCTGTG 
GCTGCTCTGT TCATCGCCCA ATACCGTGAG ATGAGCTATT CCTTCGGCAC CATTGTGGCC 
GTCAGCATAA CAGCCACGGC GGCATCGATT GGAGCTGCTG GAATCCCGCA GGCTGGACTT 
GTTACCATGG TCATGGTGCT GGACACAGTG GGCTTGGAGC CGAAGGATGT GTCCCTCATC 
ATAGCCGTCG ATTGGCTACT GGATCGCTTC CGCACCACCA TTAATGTAAT GTGCGATGCT 
CTAGGCACTA TTTTGGTTAA CCATCTGTCG AAGAATGATT TGGCCAGCGT GGATAGGCTG 
AATGCCGAGC CCCATGAGCT CCTCGAGCTG GGACCCAATG GCCACGAGAT GAAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477428.1
CDS	410..1849
Translation	MTRPKQDGGKFKAFMQENVLTMATVIGVFVGGLIGFIIKNSTGEWSKREIMYISFPGEIFLRMLKCLIVPLLVSSITSAIGGLDLSMSSKIATRAITYYFVTTISAVILGICLVTTLRPGQGAKIVETQTESIDKASKVLTPDTLMDLVRNMFTDNIIQSTMFQHRTEIYENTSISPAQPMENWEFKSAQREGSNVLGLVMFSVILGTTIGRMREKGQLLQDFFTTLSEAMMTITSWVIWISPLGVAFLIAAKIIEMESIAATIQSLGWYFITVMIGLFLHGFGTIAVIFFLGTRRLPYRYIAKLSQVLATAFGTGSSSATMPLTIKCLDNMGIDPRVTRFVIPVGATINMDGTALYEAVAALFIAQYREMSYSFGTIVAVSITATAASIGAAGIPQAGLVTMVMVLDTVGLEPKDVSLIIAVDWLLDRFRTTINVMCDALGTILVNHLSKNDLASVDRLNAEPHELLELGPNGHEMKE

Target ORF information:

RefSeq Version	NM_058080.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster excitatory amino acid transporter 1, transcript variant A (Eaat1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058080.4

ORF Insert Sequence:

ATGACGCGAC CCAAACAGGA TGGCGGCAAG TTCAAGGCCT TCATGCAGGA GAATGTCCTT 
ACCATGGCCA CCGTTATCGG TGTGTTTGTG GGTGGACTCA TCGGCTTCAT CATCAAAAAT 
AGCACTGGCG AGTGGTCGAA GAGAGAGATC ATGTACATAT CCTTCCCCGG CGAGATTTTC 
TTGCGAATGC TTAAATGTTT GATTGTGCCG CTTTTGGTCT CATCAATCAC CAGTGCCATT 
GGTGGACTCG ACCTGAGCAT GTCCAGCAAG ATTGCTACCA GAGCCATTAC TTACTACTTT 
GTGACCACCA TATCGGCCGT GATTCTGGGA ATATGTCTGG TGACCACACT GCGTCCCGGC 
CAGGGAGCCA AGATCGTGGA GACCCAGACG GAGAGCATTG ATAAGGCATC GAAGGTGCTC 
ACCCCAGACA CGCTTATGGA TTTGGTGCGA AACATGTTCA CGGACAACAT CATTCAGTCG 
ACCATGTTCC AGCACCGCAC TGAGATCTAT GAGAACACTA GCATTAGCCC AGCACAGCCT 
ATGGAAAACT GGGAGTTCAA GTCGGCTCAG CGCGAGGGTT CTAATGTCCT GGGTCTTGTG 
ATGTTCAGTG TTATCCTAGG TACCACCATT GGAAGAATGC GGGAGAAGGG ACAACTGCTG 
CAGGATTTCT TCACCACACT GAGCGAGGCA ATGATGACCA TCACCTCATG GGTTATTTGG 
ATTTCCCCGC TGGGTGTTGC CTTTCTGATA GCCGCCAAGA TCATTGAGAT GGAATCGATA 
GCAGCAACGA TTCAGTCATT AGGATGGTAT TTCATAACGG TCATGATAGG TCTATTCCTT 
CACGGTTTTG GTACGATTGC GGTGATCTTC TTCCTGGGCA CCCGACGTCT CCCGTACCGC 
TATATTGCCA AGCTTAGTCA GGTCCTGGCA ACTGCATTTG GAACAGGTTC CAGCTCGGCC 
ACCATGCCGC TGACCATCAA GTGTTTGGAC AACATGGGCA TCGATCCGCG GGTTACTCGA 
TTTGTCATTC CTGTTGGTGC CACAATTAAC ATGGACGGAA CGGCTCTCTA TGAGGCTGTG 
GCTGCTCTGT TCATCGCCCA ATACCGTGAG ATGAGCTATT CCTTCGGCAC CATTGTGGCC 
GTCAGCATAA CAGCCACGGC GGCATCGATT GGAGCTGCTG GAATCCCGCA GGCTGGACTT 
GTTACCATGG TCATGGTGCT GGACACAGTG GGCTTGGAGC CGAAGGATGT GTCCCTCATC 
ATAGCCGTCG ATTGGCTACT GGATCGCTTC CGCACCACCA TTAATGTAAT GTGCGATGCT 
CTAGGCACTA TTTTGGTTAA CCATCTGTCG AAGAATGATT TGGCCAGCGT GGATAGGCTG 
AATGCCGAGC CCCATGAGCT CCTCGAGCTG GGACCCAATG GCCACGAGAT GAAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eaat1 ( NM_164853.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723454.1 Drosophila melanogaster excitatory amino acid transporter 1, transcript variant B (Eaat1), mRNA.

Price & Availability↑

CloneID	OFa14186
Clone ID Related Accession (Same CDS sequence)	NM_058080.4 , NM_164853.3 , NM_164854.3 , NM_001201822.2
Accession Version	NM_164853.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	excitatory amino acid transporter 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164853.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCGAC CCAAACAGGA TGGCGGCAAG TTCAAGGCCT TCATGCAGGA GAATGTCCTT 
ACCATGGCCA CCGTTATCGG TGTGTTTGTG GGTGGACTCA TCGGCTTCAT CATCAAAAAT 
AGCACTGGCG AGTGGTCGAA GAGAGAGATC ATGTACATAT CCTTCCCCGG CGAGATTTTC 
TTGCGAATGC TTAAATGTTT GATTGTGCCG CTTTTGGTCT CATCAATCAC CAGTGCCATT 
GGTGGACTCG ACCTGAGCAT GTCCAGCAAG ATTGCTACCA GAGCCATTAC TTACTACTTT 
GTGACCACCA TATCGGCCGT GATTCTGGGA ATATGTCTGG TGACCACACT GCGTCCCGGC 
CAGGGAGCCA AGATCGTGGA GACCCAGACG GAGAGCATTG ATAAGGCATC GAAGGTGCTC 
ACCCCAGACA CGCTTATGGA TTTGGTGCGA AACATGTTCA CGGACAACAT CATTCAGTCG 
ACCATGTTCC AGCACCGCAC TGAGATCTAT GAGAACACTA GCATTAGCCC AGCACAGCCT 
ATGGAAAACT GGGAGTTCAA GTCGGCTCAG CGCGAGGGTT CTAATGTCCT GGGTCTTGTG 
ATGTTCAGTG TTATCCTAGG TACCACCATT GGAAGAATGC GGGAGAAGGG ACAACTGCTG 
CAGGATTTCT TCACCACACT GAGCGAGGCA ATGATGACCA TCACCTCATG GGTTATTTGG 
ATTTCCCCGC TGGGTGTTGC CTTTCTGATA GCCGCCAAGA TCATTGAGAT GGAATCGATA 
GCAGCAACGA TTCAGTCATT AGGATGGTAT TTCATAACGG TCATGATAGG TCTATTCCTT 
CACGGTTTTG GTACGATTGC GGTGATCTTC TTCCTGGGCA CCCGACGTCT CCCGTACCGC 
TATATTGCCA AGCTTAGTCA GGTCCTGGCA ACTGCATTTG GAACAGGTTC CAGCTCGGCC 
ACCATGCCGC TGACCATCAA GTGTTTGGAC AACATGGGCA TCGATCCGCG GGTTACTCGA 
TTTGTCATTC CTGTTGGTGC CACAATTAAC ATGGACGGAA CGGCTCTCTA TGAGGCTGTG 
GCTGCTCTGT TCATCGCCCA ATACCGTGAG ATGAGCTATT CCTTCGGCAC CATTGTGGCC 
GTCAGCATAA CAGCCACGGC GGCATCGATT GGAGCTGCTG GAATCCCGCA GGCTGGACTT 
GTTACCATGG TCATGGTGCT GGACACAGTG GGCTTGGAGC CGAAGGATGT GTCCCTCATC 
ATAGCCGTCG ATTGGCTACT GGATCGCTTC CGCACCACCA TTAATGTAAT GTGCGATGCT 
CTAGGCACTA TTTTGGTTAA CCATCTGTCG AAGAATGATT TGGCCAGCGT GGATAGGCTG 
AATGCCGAGC CCCATGAGCT CCTCGAGCTG GGACCCAATG GCCACGAGAT GAAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723454.1
CDS	214..1653
Translation	MTRPKQDGGKFKAFMQENVLTMATVIGVFVGGLIGFIIKNSTGEWSKREIMYISFPGEIFLRMLKCLIVPLLVSSITSAIGGLDLSMSSKIATRAITYYFVTTISAVILGICLVTTLRPGQGAKIVETQTESIDKASKVLTPDTLMDLVRNMFTDNIIQSTMFQHRTEIYENTSISPAQPMENWEFKSAQREGSNVLGLVMFSVILGTTIGRMREKGQLLQDFFTTLSEAMMTITSWVIWISPLGVAFLIAAKIIEMESIAATIQSLGWYFITVMIGLFLHGFGTIAVIFFLGTRRLPYRYIAKLSQVLATAFGTGSSSATMPLTIKCLDNMGIDPRVTRFVIPVGATINMDGTALYEAVAALFIAQYREMSYSFGTIVAVSITATAASIGAAGIPQAGLVTMVMVLDTVGLEPKDVSLIIAVDWLLDRFRTTINVMCDALGTILVNHLSKNDLASVDRLNAEPHELLELGPNGHEMKE

Target ORF information:

RefSeq Version	NM_164853.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster excitatory amino acid transporter 1, transcript variant B (Eaat1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164853.3

ORF Insert Sequence:

ATGACGCGAC CCAAACAGGA TGGCGGCAAG TTCAAGGCCT TCATGCAGGA GAATGTCCTT 
ACCATGGCCA CCGTTATCGG TGTGTTTGTG GGTGGACTCA TCGGCTTCAT CATCAAAAAT 
AGCACTGGCG AGTGGTCGAA GAGAGAGATC ATGTACATAT CCTTCCCCGG CGAGATTTTC 
TTGCGAATGC TTAAATGTTT GATTGTGCCG CTTTTGGTCT CATCAATCAC CAGTGCCATT 
GGTGGACTCG ACCTGAGCAT GTCCAGCAAG ATTGCTACCA GAGCCATTAC TTACTACTTT 
GTGACCACCA TATCGGCCGT GATTCTGGGA ATATGTCTGG TGACCACACT GCGTCCCGGC 
CAGGGAGCCA AGATCGTGGA GACCCAGACG GAGAGCATTG ATAAGGCATC GAAGGTGCTC 
ACCCCAGACA CGCTTATGGA TTTGGTGCGA AACATGTTCA CGGACAACAT CATTCAGTCG 
ACCATGTTCC AGCACCGCAC TGAGATCTAT GAGAACACTA GCATTAGCCC AGCACAGCCT 
ATGGAAAACT GGGAGTTCAA GTCGGCTCAG CGCGAGGGTT CTAATGTCCT GGGTCTTGTG 
ATGTTCAGTG TTATCCTAGG TACCACCATT GGAAGAATGC GGGAGAAGGG ACAACTGCTG 
CAGGATTTCT TCACCACACT GAGCGAGGCA ATGATGACCA TCACCTCATG GGTTATTTGG 
ATTTCCCCGC TGGGTGTTGC CTTTCTGATA GCCGCCAAGA TCATTGAGAT GGAATCGATA 
GCAGCAACGA TTCAGTCATT AGGATGGTAT TTCATAACGG TCATGATAGG TCTATTCCTT 
CACGGTTTTG GTACGATTGC GGTGATCTTC TTCCTGGGCA CCCGACGTCT CCCGTACCGC 
TATATTGCCA AGCTTAGTCA GGTCCTGGCA ACTGCATTTG GAACAGGTTC CAGCTCGGCC 
ACCATGCCGC TGACCATCAA GTGTTTGGAC AACATGGGCA TCGATCCGCG GGTTACTCGA 
TTTGTCATTC CTGTTGGTGC CACAATTAAC ATGGACGGAA CGGCTCTCTA TGAGGCTGTG 
GCTGCTCTGT TCATCGCCCA ATACCGTGAG ATGAGCTATT CCTTCGGCAC CATTGTGGCC 
GTCAGCATAA CAGCCACGGC GGCATCGATT GGAGCTGCTG GAATCCCGCA GGCTGGACTT 
GTTACCATGG TCATGGTGCT GGACACAGTG GGCTTGGAGC CGAAGGATGT GTCCCTCATC 
ATAGCCGTCG ATTGGCTACT GGATCGCTTC CGCACCACCA TTAATGTAAT GTGCGATGCT 
CTAGGCACTA TTTTGGTTAA CCATCTGTCG AAGAATGATT TGGCCAGCGT GGATAGGCTG 
AATGCCGAGC CCCATGAGCT CCTCGAGCTG GGACCCAATG GCCACGAGAT GAAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eaat1 ( NM_164854.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723455.1 Drosophila melanogaster excitatory amino acid transporter 1, transcript variant C (Eaat1), mRNA.

Price & Availability↑

CloneID	OFa14186
Clone ID Related Accession (Same CDS sequence)	NM_058080.4 , NM_164853.3 , NM_164854.3 , NM_001201822.2
Accession Version	NM_164854.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	excitatory amino acid transporter 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164854.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCGAC CCAAACAGGA TGGCGGCAAG TTCAAGGCCT TCATGCAGGA GAATGTCCTT 
ACCATGGCCA CCGTTATCGG TGTGTTTGTG GGTGGACTCA TCGGCTTCAT CATCAAAAAT 
AGCACTGGCG AGTGGTCGAA GAGAGAGATC ATGTACATAT CCTTCCCCGG CGAGATTTTC 
TTGCGAATGC TTAAATGTTT GATTGTGCCG CTTTTGGTCT CATCAATCAC CAGTGCCATT 
GGTGGACTCG ACCTGAGCAT GTCCAGCAAG ATTGCTACCA GAGCCATTAC TTACTACTTT 
GTGACCACCA TATCGGCCGT GATTCTGGGA ATATGTCTGG TGACCACACT GCGTCCCGGC 
CAGGGAGCCA AGATCGTGGA GACCCAGACG GAGAGCATTG ATAAGGCATC GAAGGTGCTC 
ACCCCAGACA CGCTTATGGA TTTGGTGCGA AACATGTTCA CGGACAACAT CATTCAGTCG 
ACCATGTTCC AGCACCGCAC TGAGATCTAT GAGAACACTA GCATTAGCCC AGCACAGCCT 
ATGGAAAACT GGGAGTTCAA GTCGGCTCAG CGCGAGGGTT CTAATGTCCT GGGTCTTGTG 
ATGTTCAGTG TTATCCTAGG TACCACCATT GGAAGAATGC GGGAGAAGGG ACAACTGCTG 
CAGGATTTCT TCACCACACT GAGCGAGGCA ATGATGACCA TCACCTCATG GGTTATTTGG 
ATTTCCCCGC TGGGTGTTGC CTTTCTGATA GCCGCCAAGA TCATTGAGAT GGAATCGATA 
GCAGCAACGA TTCAGTCATT AGGATGGTAT TTCATAACGG TCATGATAGG TCTATTCCTT 
CACGGTTTTG GTACGATTGC GGTGATCTTC TTCCTGGGCA CCCGACGTCT CCCGTACCGC 
TATATTGCCA AGCTTAGTCA GGTCCTGGCA ACTGCATTTG GAACAGGTTC CAGCTCGGCC 
ACCATGCCGC TGACCATCAA GTGTTTGGAC AACATGGGCA TCGATCCGCG GGTTACTCGA 
TTTGTCATTC CTGTTGGTGC CACAATTAAC ATGGACGGAA CGGCTCTCTA TGAGGCTGTG 
GCTGCTCTGT TCATCGCCCA ATACCGTGAG ATGAGCTATT CCTTCGGCAC CATTGTGGCC 
GTCAGCATAA CAGCCACGGC GGCATCGATT GGAGCTGCTG GAATCCCGCA GGCTGGACTT 
GTTACCATGG TCATGGTGCT GGACACAGTG GGCTTGGAGC CGAAGGATGT GTCCCTCATC 
ATAGCCGTCG ATTGGCTACT GGATCGCTTC CGCACCACCA TTAATGTAAT GTGCGATGCT 
CTAGGCACTA TTTTGGTTAA CCATCTGTCG AAGAATGATT TGGCCAGCGT GGATAGGCTG 
AATGCCGAGC CCCATGAGCT CCTCGAGCTG GGACCCAATG GCCACGAGAT GAAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723455.1
CDS	276..1715
Translation	MTRPKQDGGKFKAFMQENVLTMATVIGVFVGGLIGFIIKNSTGEWSKREIMYISFPGEIFLRMLKCLIVPLLVSSITSAIGGLDLSMSSKIATRAITYYFVTTISAVILGICLVTTLRPGQGAKIVETQTESIDKASKVLTPDTLMDLVRNMFTDNIIQSTMFQHRTEIYENTSISPAQPMENWEFKSAQREGSNVLGLVMFSVILGTTIGRMREKGQLLQDFFTTLSEAMMTITSWVIWISPLGVAFLIAAKIIEMESIAATIQSLGWYFITVMIGLFLHGFGTIAVIFFLGTRRLPYRYIAKLSQVLATAFGTGSSSATMPLTIKCLDNMGIDPRVTRFVIPVGATINMDGTALYEAVAALFIAQYREMSYSFGTIVAVSITATAASIGAAGIPQAGLVTMVMVLDTVGLEPKDVSLIIAVDWLLDRFRTTINVMCDALGTILVNHLSKNDLASVDRLNAEPHELLELGPNGHEMKE

Target ORF information:

RefSeq Version	NM_164854.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster excitatory amino acid transporter 1, transcript variant C (Eaat1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164854.3

ORF Insert Sequence:

ATGACGCGAC CCAAACAGGA TGGCGGCAAG TTCAAGGCCT TCATGCAGGA GAATGTCCTT 
ACCATGGCCA CCGTTATCGG TGTGTTTGTG GGTGGACTCA TCGGCTTCAT CATCAAAAAT 
AGCACTGGCG AGTGGTCGAA GAGAGAGATC ATGTACATAT CCTTCCCCGG CGAGATTTTC 
TTGCGAATGC TTAAATGTTT GATTGTGCCG CTTTTGGTCT CATCAATCAC CAGTGCCATT 
GGTGGACTCG ACCTGAGCAT GTCCAGCAAG ATTGCTACCA GAGCCATTAC TTACTACTTT 
GTGACCACCA TATCGGCCGT GATTCTGGGA ATATGTCTGG TGACCACACT GCGTCCCGGC 
CAGGGAGCCA AGATCGTGGA GACCCAGACG GAGAGCATTG ATAAGGCATC GAAGGTGCTC 
ACCCCAGACA CGCTTATGGA TTTGGTGCGA AACATGTTCA CGGACAACAT CATTCAGTCG 
ACCATGTTCC AGCACCGCAC TGAGATCTAT GAGAACACTA GCATTAGCCC AGCACAGCCT 
ATGGAAAACT GGGAGTTCAA GTCGGCTCAG CGCGAGGGTT CTAATGTCCT GGGTCTTGTG 
ATGTTCAGTG TTATCCTAGG TACCACCATT GGAAGAATGC GGGAGAAGGG ACAACTGCTG 
CAGGATTTCT TCACCACACT GAGCGAGGCA ATGATGACCA TCACCTCATG GGTTATTTGG 
ATTTCCCCGC TGGGTGTTGC CTTTCTGATA GCCGCCAAGA TCATTGAGAT GGAATCGATA 
GCAGCAACGA TTCAGTCATT AGGATGGTAT TTCATAACGG TCATGATAGG TCTATTCCTT 
CACGGTTTTG GTACGATTGC GGTGATCTTC TTCCTGGGCA CCCGACGTCT CCCGTACCGC 
TATATTGCCA AGCTTAGTCA GGTCCTGGCA ACTGCATTTG GAACAGGTTC CAGCTCGGCC 
ACCATGCCGC TGACCATCAA GTGTTTGGAC AACATGGGCA TCGATCCGCG GGTTACTCGA 
TTTGTCATTC CTGTTGGTGC CACAATTAAC ATGGACGGAA CGGCTCTCTA TGAGGCTGTG 
GCTGCTCTGT TCATCGCCCA ATACCGTGAG ATGAGCTATT CCTTCGGCAC CATTGTGGCC 
GTCAGCATAA CAGCCACGGC GGCATCGATT GGAGCTGCTG GAATCCCGCA GGCTGGACTT 
GTTACCATGG TCATGGTGCT GGACACAGTG GGCTTGGAGC CGAAGGATGT GTCCCTCATC 
ATAGCCGTCG ATTGGCTACT GGATCGCTTC CGCACCACCA TTAATGTAAT GTGCGATGCT 
CTAGGCACTA TTTTGGTTAA CCATCTGTCG AAGAATGATT TGGCCAGCGT GGATAGGCTG 
AATGCCGAGC CCCATGAGCT CCTCGAGCTG GGACCCAATG GCCACGAGAT GAAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eaat1 ( NM_001201822.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188751.1 Drosophila melanogaster excitatory amino acid transporter 1, transcript variant D (Eaat1), mRNA.

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CloneID	OFa14186
Clone ID Related Accession (Same CDS sequence)	NM_058080.4 , NM_164853.3 , NM_164854.3 , NM_001201822.2
Accession Version	NM_001201822.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	excitatory amino acid transporter 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001201822.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCGAC CCAAACAGGA TGGCGGCAAG TTCAAGGCCT TCATGCAGGA GAATGTCCTT 
ACCATGGCCA CCGTTATCGG TGTGTTTGTG GGTGGACTCA TCGGCTTCAT CATCAAAAAT 
AGCACTGGCG AGTGGTCGAA GAGAGAGATC ATGTACATAT CCTTCCCCGG CGAGATTTTC 
TTGCGAATGC TTAAATGTTT GATTGTGCCG CTTTTGGTCT CATCAATCAC CAGTGCCATT 
GGTGGACTCG ACCTGAGCAT GTCCAGCAAG ATTGCTACCA GAGCCATTAC TTACTACTTT 
GTGACCACCA TATCGGCCGT GATTCTGGGA ATATGTCTGG TGACCACACT GCGTCCCGGC 
CAGGGAGCCA AGATCGTGGA GACCCAGACG GAGAGCATTG ATAAGGCATC GAAGGTGCTC 
ACCCCAGACA CGCTTATGGA TTTGGTGCGA AACATGTTCA CGGACAACAT CATTCAGTCG 
ACCATGTTCC AGCACCGCAC TGAGATCTAT GAGAACACTA GCATTAGCCC AGCACAGCCT 
ATGGAAAACT GGGAGTTCAA GTCGGCTCAG CGCGAGGGTT CTAATGTCCT GGGTCTTGTG 
ATGTTCAGTG TTATCCTAGG TACCACCATT GGAAGAATGC GGGAGAAGGG ACAACTGCTG 
CAGGATTTCT TCACCACACT GAGCGAGGCA ATGATGACCA TCACCTCATG GGTTATTTGG 
ATTTCCCCGC TGGGTGTTGC CTTTCTGATA GCCGCCAAGA TCATTGAGAT GGAATCGATA 
GCAGCAACGA TTCAGTCATT AGGATGGTAT TTCATAACGG TCATGATAGG TCTATTCCTT 
CACGGTTTTG GTACGATTGC GGTGATCTTC TTCCTGGGCA CCCGACGTCT CCCGTACCGC 
TATATTGCCA AGCTTAGTCA GGTCCTGGCA ACTGCATTTG GAACAGGTTC CAGCTCGGCC 
ACCATGCCGC TGACCATCAA GTGTTTGGAC AACATGGGCA TCGATCCGCG GGTTACTCGA 
TTTGTCATTC CTGTTGGTGC CACAATTAAC ATGGACGGAA CGGCTCTCTA TGAGGCTGTG 
GCTGCTCTGT TCATCGCCCA ATACCGTGAG ATGAGCTATT CCTTCGGCAC CATTGTGGCC 
GTCAGCATAA CAGCCACGGC GGCATCGATT GGAGCTGCTG GAATCCCGCA GGCTGGACTT 
GTTACCATGG TCATGGTGCT GGACACAGTG GGCTTGGAGC CGAAGGATGT GTCCCTCATC 
ATAGCCGTCG ATTGGCTACT GGATCGCTTC CGCACCACCA TTAATGTAAT GTGCGATGCT 
CTAGGCACTA TTTTGGTTAA CCATCTGTCG AAGAATGATT TGGCCAGCGT GGATAGGCTG 
AATGCCGAGC CCCATGAGCT CCTCGAGCTG GGACCCAATG GCCACGAGAT GAAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188751.1
CDS	382..1821
Translation	MTRPKQDGGKFKAFMQENVLTMATVIGVFVGGLIGFIIKNSTGEWSKREIMYISFPGEIFLRMLKCLIVPLLVSSITSAIGGLDLSMSSKIATRAITYYFVTTISAVILGICLVTTLRPGQGAKIVETQTESIDKASKVLTPDTLMDLVRNMFTDNIIQSTMFQHRTEIYENTSISPAQPMENWEFKSAQREGSNVLGLVMFSVILGTTIGRMREKGQLLQDFFTTLSEAMMTITSWVIWISPLGVAFLIAAKIIEMESIAATIQSLGWYFITVMIGLFLHGFGTIAVIFFLGTRRLPYRYIAKLSQVLATAFGTGSSSATMPLTIKCLDNMGIDPRVTRFVIPVGATINMDGTALYEAVAALFIAQYREMSYSFGTIVAVSITATAASIGAAGIPQAGLVTMVMVLDTVGLEPKDVSLIIAVDWLLDRFRTTINVMCDALGTILVNHLSKNDLASVDRLNAEPHELLELGPNGHEMKE

Target ORF information:

RefSeq Version	NM_001201822.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster excitatory amino acid transporter 1, transcript variant D (Eaat1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201822.2

ORF Insert Sequence:

ATGACGCGAC CCAAACAGGA TGGCGGCAAG TTCAAGGCCT TCATGCAGGA GAATGTCCTT 
ACCATGGCCA CCGTTATCGG TGTGTTTGTG GGTGGACTCA TCGGCTTCAT CATCAAAAAT 
AGCACTGGCG AGTGGTCGAA GAGAGAGATC ATGTACATAT CCTTCCCCGG CGAGATTTTC 
TTGCGAATGC TTAAATGTTT GATTGTGCCG CTTTTGGTCT CATCAATCAC CAGTGCCATT 
GGTGGACTCG ACCTGAGCAT GTCCAGCAAG ATTGCTACCA GAGCCATTAC TTACTACTTT 
GTGACCACCA TATCGGCCGT GATTCTGGGA ATATGTCTGG TGACCACACT GCGTCCCGGC 
CAGGGAGCCA AGATCGTGGA GACCCAGACG GAGAGCATTG ATAAGGCATC GAAGGTGCTC 
ACCCCAGACA CGCTTATGGA TTTGGTGCGA AACATGTTCA CGGACAACAT CATTCAGTCG 
ACCATGTTCC AGCACCGCAC TGAGATCTAT GAGAACACTA GCATTAGCCC AGCACAGCCT 
ATGGAAAACT GGGAGTTCAA GTCGGCTCAG CGCGAGGGTT CTAATGTCCT GGGTCTTGTG 
ATGTTCAGTG TTATCCTAGG TACCACCATT GGAAGAATGC GGGAGAAGGG ACAACTGCTG 
CAGGATTTCT TCACCACACT GAGCGAGGCA ATGATGACCA TCACCTCATG GGTTATTTGG 
ATTTCCCCGC TGGGTGTTGC CTTTCTGATA GCCGCCAAGA TCATTGAGAT GGAATCGATA 
GCAGCAACGA TTCAGTCATT AGGATGGTAT TTCATAACGG TCATGATAGG TCTATTCCTT 
CACGGTTTTG GTACGATTGC GGTGATCTTC TTCCTGGGCA CCCGACGTCT CCCGTACCGC 
TATATTGCCA AGCTTAGTCA GGTCCTGGCA ACTGCATTTG GAACAGGTTC CAGCTCGGCC 
ACCATGCCGC TGACCATCAA GTGTTTGGAC AACATGGGCA TCGATCCGCG GGTTACTCGA 
TTTGTCATTC CTGTTGGTGC CACAATTAAC ATGGACGGAA CGGCTCTCTA TGAGGCTGTG 
GCTGCTCTGT TCATCGCCCA ATACCGTGAG ATGAGCTATT CCTTCGGCAC CATTGTGGCC 
GTCAGCATAA CAGCCACGGC GGCATCGATT GGAGCTGCTG GAATCCCGCA GGCTGGACTT 
GTTACCATGG TCATGGTGCT GGACACAGTG GGCTTGGAGC CGAAGGATGT GTCCCTCATC 
ATAGCCGTCG ATTGGCTACT GGATCGCTTC CGCACCACCA TTAATGTAAT GTGCGATGCT 
CTAGGCACTA TTTTGGTTAA CCATCTGTCG AAGAATGATT TGGCCAGCGT GGATAGGCTG 
AATGCCGAGC CCCATGAGCT CCTCGAGCTG GGACCCAATG GCCACGAGAT GAAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Eaat1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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