emb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	emb
Entrez Gene ID	34167
Full Name	embargoed
Synonyms	CG13387,CRM1,Crm1,DCRM1,Dmel\CG13387,Emb,XPO-1,XPO1,Xpo1,crm1,dCRM1,dXPO1,l(2)k16715
General protein information	Preferred Names embargoed Names CG13387 gene product from transcript CG13387-RA CG13387-PA CG13387-PB Exportin-1 chromosomal region maintenance 1 emb-PA emb-PB exportin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 29C1-29C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_164818.2	NP_723391.2	embargoed, isoform A
NM_001316387.1	NP_001303316.1	embargoed, isoform B

Pathways from BioSystems

Homologies

	XPO1	XP_001115623.1
Mus musculus (house mouse)	Xpo1	NP_001030303.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009929	XP_319051.2
Saccharomyces cerevisiae (baker's yeast)	CRM1	NP_011734.3
Eremothecium gossypii	AGOS_ADL128C	NP_983968.2
Neurospora crassa	NCU01820	XP_955917.2
Arabidopsis thaliana (thale cress)	XPO1B	NP_566193.2
Arabidopsis thaliana (thale cress)	XPO1A	NP_197204.1
Oryza sativa (rice)	Os03g0858100	NP_001051959.1
Bos taurus (cattle)	XPO1	NP_001179190.1
Kluyveromyces lactis	KLLA0E16061g	XP_454672.1
Magnaporthe oryzae (rice blast fungus)	MGG_02526	XP_003709336.1
Rattus norvegicus (Norway rat)	Xpo1	NP_445942.1
Drosophila melanogaster (fruit fly)	emb	NP_723391.2
Homo sapiens (human)	XPO1	NP_003391.1
Danio rerio (zebrafish)	xpo1b	XP_001919797.3
Caenorhabditis elegans (roundworm)	xpo-1	NP_741567.1
Xenopus tropicalis (tropical clawed frog)	xpo1	XP_002939551.2
Pan troglodytes (chimpanzee)	XPO1	XP_515727.2
Canis lupus familiaris (dog)	XPO1	XP_005626195.1
Gallus gallus (chicken)	XPO1	NP_001012887.2
Schizosaccharomyces pombe (fission yeast)	crm1	XP_001713070.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

two newly identified factors that restrict diverse viruses, dXPO1 and dRUVBL1 contributed to antiviral defense at the organismal level in adult flies
Title: Genome-wide RNAi screen identifies broadly-acting host factors that inhibit arbovirus infection.

HPO promotes the translocation of SD to the cytoplasm in a CRM1-dependent manner
Title: The Hippo kinase promotes Scalloped cytoplasmic localization independently of Warts in a CRM1/Exportin1-dependent manner in Drosophila.

Nuclear export of TIS11 proteins is mediated by CRM1 through diverging nuclear export signals, while their nuclear import mechanism may rely on a highly conserved signal whose activity is negatively regulated by ZnF2 folding.
Title: A masked PY-NLS in Drosophila TIS11 and its mammalian homolog tristetraprolin.

Exportin-1 is a nuclear export receptor for expanded polyQ containing proteins.
Title: Expanded polyglutamine domain possesses nuclear export activity which modulates subcellular localization and toxicity of polyQ disease protein via exportin-1.

Nuclear localization of the ecdysteroid receptor (EcR) is increased in HeLa cells if exportin-1 (CRM1) is knocked down by siRNA against exportin.
Title: The importance of exportin and Ran for nucleocytoplasmic shuttling of the ecdysteroid receptor.

The following emb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the emb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16476	NM_001316387.1 Latest version!	Drosophila melanogaster embargoed (emb), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa16476	NM_164818.2 Latest version!	Drosophila melanogaster embargoed (emb), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

emb ( NM_164818.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723391.2 Drosophila melanogaster embargoed (emb), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16476
Clone ID Related Accession (Same CDS sequence)	NM_164818.2 , NM_001316387.1
Accession Version	NM_164818.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	embargoed, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_164818.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGACAA TGTTGACATC GGACGAGGCG GGCAAGCTGC TCGACTTCTC CCAGAAGCTG 
GACATTAATC TGCTGGACAA GATCGTCGAG GTTGTGTACA CAGCGCAGGG CGAGCAGTTG 
CGTCTAGCCC AGAGCATACT GACCACGCTG AAGGAGCATC CAGAGGCATG GACGCGGGTG 
GACAGCATAT TGGAGTACTC ACAGAACCAG CGGACCAAGT TTTATGCTCT GCAAATCCTC 
GAGGAGGTCA TCAAAACGCG CTGGAAGGTC CTGCCGCGCA ACCAGTGCGA GGGAATCAAG 
AAGTATGTGG TCAGTCTTAT CATCAAGACC TCGTCGGATC CCATTGTTAT GGAGCAGAAC 
AAAGTGTATC TGAATAAGCT AAACATGATT CTGGTCCACA TTCTAAAGCG CGAATGGCCA 
CGCAACTGGG AGACATTCAT CAGTGACATT GTCGGTGCGT CGAAAACGAA CGAGAGCCTG 
TGCATGAACA ACATGGTAAT CCTGAAGAAC CTTAGTGAGG AGGTGTTCGA CTTTTCGCAG 
GGTCAGATCA CGCAGACTAA GGCCAAGCAC CTGAAAGACA CCATGTGCTC GGAGTTCTCT 
CAGATCTTTA CACTATGCTC CTTCGTGCTG GAGAACTCGA TGAATGCCGC CCTAATCCAT 
GTCACGCTGG AGACTCTGCT GAGGTTCCTT AACTGGATAC CGCTGGGCTA CATCTTTGAA 
ACGCAGCAAA TTGAAACGCT GATATTCAAA TTCCTTAGCG TACCCATGTT CCGCAATGTG 
ACCCTGAAGT GTCTGTCCGA GATCGCCGGC CTGACTGCGG CAAACTACGA CGAGAATTTC 
GCGACGCTCT TCAAGGACAC GATGGTTCAG TTGGAACAGA TAGTCGGCCA GAATATGAAC 
ATGAACCATG TGTTTAAGCA TGGTTCCGAC ACAGAGCAGG AGCTGGTCCT TAATTTGGCC 
ATGTTCCTGT GCACCTTCCT CAAGGAGCAC GGCAAACTGG TGGAGGATGC CAAGTATGTG 
GACTACCTGA ATCAGGCGCT CATGTATTTG GTGATGATTA GCGAGGTGGA GGACGTGGAA 
GTATTCAAGA TCTGTCTGGA ATACTGGAAC AGCCTTGTGG AGGATCTATA CAATTCGGAA 
TTCTTCCACC CCACCCTGGA GTCTACAAAG AGGCAACAAG TGTATCCCCG TCGACGTTTC 
TACGCCCCCA TCCTGTCCAA AGTGCGATTC ATTATGATCT CGCGCATGGC CAAGCCGGAG 
GAGGTGCTGG TGGTGGAGAA CGAAAACGGG GAAGTGGTGC GCGAGTTCAT GAAGGACACA 
AACTCAATAA ACCTGTACAA GAACATGCGC GAAACGCTGG TCTTTCTTAC CCATTTGGAC 
TCTGTGGACA CGGACCGCAT CATGACGCTA AAGCTGCTTA ATCAGGTGAA CGGATCGGAA 
TTCTCTTGGA AGAACCTGAA CACGTTGTGC TGGGCCATTG GTTCCATATC CGGTGCTTTT 
TGTGAAGAGG ACGAAAAACG GTTCCTGGTC ACCGTGATCA AGGATCTATT AGGCCTGTGC 
GAACAGAAGA AGGGCAAGGA CAACAAGGCC ATTATTGCCT CCAATATCAT GTATGTGGTG 
GGACAGTATC CACGCTTCCT GCGCGCCCAT TGGAAGTTCC TCAAGACGGT GGTAAACAAA 
CTCTTCGAAT TTATGCACGA AACGCACGAT GGTGTCCAGG ATATGGCATG CGATACGTTC 
ATCAAGATAG CCATCAAATG CCGCCGCTAC TTTGTCACCA TCCAGCCGAA TGAGGCATGC 
ACCTTTATTG ACGAGATTCT CACAACAATG AGCAGCATCA TTTGTGATCT GCAGCCGCAG 
CAGGTTCACA CGTTCTATGA AGCTGTCGGC TACATGATCT CCGCCCAGGT GGATCAGGTA 
CAACAGGACG TCCTCATCGA GAGGTACATG CAACTGCCCA ACCAGGTGTG GGATGACATC 
ATCTCCCGCG CCTCGAAAAA CGTTGACTTC TTGAAGAACA TGACCGCTGT CAAGCAGCTG 
GGCAGCATCC TTAAAACGAA TGTGGCTGCC TGCAAAGCCC TGGGTCACGC GTATGTCATT 
CAACTGGGCC GCATATATCT GGACATGCTG AATGTGTACA AGATAACATC AGAGAATATC 
ATTCAGGCCA TCGAGGTGAA TGGCGTCAAT GTGAACAATC AGCCCCTGAT CAAAACCATG 
CATGTGGTCA AGAAGGAGAC GCTTAACTTG ATATCCGAGT GGGTGTCTCG TTCCAACGAC 
AATCAGTTGG TGATGGACAA TTTCATACCG CCTTTGCTGG ACGCTATACT GCTAGATTAT 
CAGCGCTGCA AAGTTCCATC GGCACGGGAG CCCAAGGTTT TAAGTGCGAT GGCAATCATT 
GTGCATAAAC TGCGTCAGCA TATCACAAAT GAAGTACCAA AAATATTTGA CGCCGTCTTC 
GAGTGCACGC TGGACATGAT CAACAAGAAC TTTGAAGATT TCCCACAGCA CAGACTTAGT 
TTCTACGAGC TACTGCAGGC GGTCAATGCG CACTGCTTTA AGGCGTTCCT TAATATACCG 
CCGGCTCAGT TCAAACTGGT CTTCGACTCG GTGGTGTGGG CATTCAAGCA TACCATGCGG 
AATGTGGCCG ACATGGGATT GAATATCCTA TTTAAGATGC TTCAAAACTT AGATCAGCAT 
CCCGGTGCGG CGCAGAGCTT CTATCAGACG TACTTTACAG ATATTCTAAT GCAGATATTT 
TCGGTGGTCA CTGACACCTC ACACACGGCC GGGTTGCCCA ATCATGCCAT CATTCTGGCG 
TACATGTTCT CACTGGTGGA GAACAGGAAG ATTACGGTGA ACCTGGGTCC GATTCCGGAT 
AATATGATAT TCATACAGGA ATATGTCGCG TCGCTGCTGA AATCGGCATT TACCCATCTG 
TCCGATAATC AGGTCAAAGT CTTTGTCACT GGCTTATTCA ATTTAGACGA GAATGTGCAA 
GCATTTAAGG AGCATTTGAG AGATTTTCTC ATACAGATTC GCGAGGCCAC CGGCGAGGAC 
GATTCCGATC TGTACTTGGA AGAGCGTGAA GCGGCTCTGG CCGAGGAGCA GTCAAATAAA 
CATCAGATGC AGCGTAATAT TCCAGGAATG TTGAATCCGC ACGAGTTGCC CGAGGATATG 
CAGGACGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723391.2
CDS	372..3563
Translation	MATMLTSDEAGKLLDFSQKLDINLLDKIVEVVYTAQGEQLRLAQSILTTLKEHPEAWTRVDSILEYSQNQRTKFYALQILEEVIKTRWKVLPRNQCEGIKKYVVSLIIKTSSDPIVMEQNKVYLNKLNMILVHILKREWPRNWETFISDIVGASKTNESLCMNNMVILKNLSEEVFDFSQGQITQTKAKHLKDTMCSEFSQIFTLCSFVLENSMNAALIHVTLETLLRFLNWIPLGYIFETQQIETLIFKFLSVPMFRNVTLKCLSEIAGLTAANYDENFATLFKDTMVQLEQIVGQNMNMNHVFKHGSDTEQELVLNLAMFLCTFLKEHGKLVEDAKYVDYLNQALMYLVMISEVEDVEVFKICLEYWNSLVEDLYNSEFFHPTLESTKRQQVYPRRRFYAPILSKVRFIMISRMAKPEEVLVVENENGEVVREFMKDTNSINLYKNMRETLVFLTHLDSVDTDRIMTLKLLNQVNGSEFSWKNLNTLCWAIGSISGAFCEEDEKRFLVTVIKDLLGLCEQKKGKDNKAIIASNIMYVVGQYPRFLRAHWKFLKTVVNKLFEFMHETHDGVQDMACDTFIKIAIKCRRYFVTIQPNEACTFIDEILTTMSSIICDLQPQQVHTFYEAVGYMISAQVDQVQQDVLIERYMQLPNQVWDDIISRASKNVDFLKNMTAVKQLGSILKTNVAACKALGHAYVIQLGRIYLDMLNVYKITSENIIQAIEVNGVNVNNQPLIKTMHVVKKETLNLISEWVSRSNDNQLVMDNFIPPLLDAILLDYQRCKVPSAREPKVLSAMAIIVHKLRQHITNEVPKIFDAVFECTLDMINKNFEDFPQHRLSFYELLQAVNAHCFKAFLNIPPAQFKLVFDSVVWAFKHTMRNVADMGLNILFKMLQNLDQHPGAAQSFYQTYFTDILMQIFSVVTDTSHTAGLPNHAIILAYMFSLVENRKITVNLGPIPDNMIFIQEYVASLLKSAFTHLSDNQVKVFVTGLFNLDENVQAFKEHLRDFLIQIREATGEDDSDLYLEEREAALAEEQSNKHQMQRNIPGMLNPHELPEDMQDE

Target ORF information:

RefSeq Version	NM_164818.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster embargoed (emb), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164818.2

ORF Insert Sequence:

ATGGCGACAA TGTTGACATC GGACGAGGCG GGCAAGCTGC TCGACTTCTC CCAGAAGCTG 
GACATTAATC TGCTGGACAA GATCGTCGAG GTTGTGTACA CAGCGCAGGG CGAGCAGTTG 
CGTCTAGCCC AGAGCATACT GACCACGCTG AAGGAGCATC CAGAGGCATG GACGCGGGTG 
GACAGCATAT TGGAGTACTC ACAGAACCAG CGGACCAAGT TTTATGCTCT GCAAATCCTC 
GAGGAGGTCA TCAAAACGCG CTGGAAGGTC CTGCCGCGCA ACCAGTGCGA GGGAATCAAG 
AAGTATGTGG TCAGTCTTAT CATCAAGACC TCGTCGGATC CCATTGTTAT GGAGCAGAAC 
AAAGTGTATC TGAATAAGCT AAACATGATT CTGGTCCACA TTCTAAAGCG CGAATGGCCA 
CGCAACTGGG AGACATTCAT CAGTGACATT GTCGGTGCGT CGAAAACGAA CGAGAGCCTG 
TGCATGAACA ACATGGTAAT CCTGAAGAAC CTTAGTGAGG AGGTGTTCGA CTTTTCGCAG 
GGTCAGATCA CGCAGACTAA GGCCAAGCAC CTGAAAGACA CCATGTGCTC GGAGTTCTCT 
CAGATCTTTA CACTATGCTC CTTCGTGCTG GAGAACTCGA TGAATGCCGC CCTAATCCAT 
GTCACGCTGG AGACTCTGCT GAGGTTCCTT AACTGGATAC CGCTGGGCTA CATCTTTGAA 
ACGCAGCAAA TTGAAACGCT GATATTCAAA TTCCTTAGCG TACCCATGTT CCGCAATGTG 
ACCCTGAAGT GTCTGTCCGA GATCGCCGGC CTGACTGCGG CAAACTACGA CGAGAATTTC 
GCGACGCTCT TCAAGGACAC GATGGTTCAG TTGGAACAGA TAGTCGGCCA GAATATGAAC 
ATGAACCATG TGTTTAAGCA TGGTTCCGAC ACAGAGCAGG AGCTGGTCCT TAATTTGGCC 
ATGTTCCTGT GCACCTTCCT CAAGGAGCAC GGCAAACTGG TGGAGGATGC CAAGTATGTG 
GACTACCTGA ATCAGGCGCT CATGTATTTG GTGATGATTA GCGAGGTGGA GGACGTGGAA 
GTATTCAAGA TCTGTCTGGA ATACTGGAAC AGCCTTGTGG AGGATCTATA CAATTCGGAA 
TTCTTCCACC CCACCCTGGA GTCTACAAAG AGGCAACAAG TGTATCCCCG TCGACGTTTC 
TACGCCCCCA TCCTGTCCAA AGTGCGATTC ATTATGATCT CGCGCATGGC CAAGCCGGAG 
GAGGTGCTGG TGGTGGAGAA CGAAAACGGG GAAGTGGTGC GCGAGTTCAT GAAGGACACA 
AACTCAATAA ACCTGTACAA GAACATGCGC GAAACGCTGG TCTTTCTTAC CCATTTGGAC 
TCTGTGGACA CGGACCGCAT CATGACGCTA AAGCTGCTTA ATCAGGTGAA CGGATCGGAA 
TTCTCTTGGA AGAACCTGAA CACGTTGTGC TGGGCCATTG GTTCCATATC CGGTGCTTTT 
TGTGAAGAGG ACGAAAAACG GTTCCTGGTC ACCGTGATCA AGGATCTATT AGGCCTGTGC 
GAACAGAAGA AGGGCAAGGA CAACAAGGCC ATTATTGCCT CCAATATCAT GTATGTGGTG 
GGACAGTATC CACGCTTCCT GCGCGCCCAT TGGAAGTTCC TCAAGACGGT GGTAAACAAA 
CTCTTCGAAT TTATGCACGA AACGCACGAT GGTGTCCAGG ATATGGCATG CGATACGTTC 
ATCAAGATAG CCATCAAATG CCGCCGCTAC TTTGTCACCA TCCAGCCGAA TGAGGCATGC 
ACCTTTATTG ACGAGATTCT CACAACAATG AGCAGCATCA TTTGTGATCT GCAGCCGCAG 
CAGGTTCACA CGTTCTATGA AGCTGTCGGC TACATGATCT CCGCCCAGGT GGATCAGGTA 
CAACAGGACG TCCTCATCGA GAGGTACATG CAACTGCCCA ACCAGGTGTG GGATGACATC 
ATCTCCCGCG CCTCGAAAAA CGTTGACTTC TTGAAGAACA TGACCGCTGT CAAGCAGCTG 
GGCAGCATCC TTAAAACGAA TGTGGCTGCC TGCAAAGCCC TGGGTCACGC GTATGTCATT 
CAACTGGGCC GCATATATCT GGACATGCTG AATGTGTACA AGATAACATC AGAGAATATC 
ATTCAGGCCA TCGAGGTGAA TGGCGTCAAT GTGAACAATC AGCCCCTGAT CAAAACCATG 
CATGTGGTCA AGAAGGAGAC GCTTAACTTG ATATCCGAGT GGGTGTCTCG TTCCAACGAC 
AATCAGTTGG TGATGGACAA TTTCATACCG CCTTTGCTGG ACGCTATACT GCTAGATTAT 
CAGCGCTGCA AAGTTCCATC GGCACGGGAG CCCAAGGTTT TAAGTGCGAT GGCAATCATT 
GTGCATAAAC TGCGTCAGCA TATCACAAAT GAAGTACCAA AAATATTTGA CGCCGTCTTC 
GAGTGCACGC TGGACATGAT CAACAAGAAC TTTGAAGATT TCCCACAGCA CAGACTTAGT 
TTCTACGAGC TACTGCAGGC GGTCAATGCG CACTGCTTTA AGGCGTTCCT TAATATACCG 
CCGGCTCAGT TCAAACTGGT CTTCGACTCG GTGGTGTGGG CATTCAAGCA TACCATGCGG 
AATGTGGCCG ACATGGGATT GAATATCCTA TTTAAGATGC TTCAAAACTT AGATCAGCAT 
CCCGGTGCGG CGCAGAGCTT CTATCAGACG TACTTTACAG ATATTCTAAT GCAGATATTT 
TCGGTGGTCA CTGACACCTC ACACACGGCC GGGTTGCCCA ATCATGCCAT CATTCTGGCG 
TACATGTTCT CACTGGTGGA GAACAGGAAG ATTACGGTGA ACCTGGGTCC GATTCCGGAT 
AATATGATAT TCATACAGGA ATATGTCGCG TCGCTGCTGA AATCGGCATT TACCCATCTG 
TCCGATAATC AGGTCAAAGT CTTTGTCACT GGCTTATTCA ATTTAGACGA GAATGTGCAA 
GCATTTAAGG AGCATTTGAG AGATTTTCTC ATACAGATTC GCGAGGCCAC CGGCGAGGAC 
GATTCCGATC TGTACTTGGA AGAGCGTGAA GCGGCTCTGG CCGAGGAGCA GTCAAATAAA 
CATCAGATGC AGCGTAATAT TCCAGGAATG TTGAATCCGC ACGAGTTGCC CGAGGATATG 
CAGGACGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emb ( NM_001316387.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303316.1 Drosophila melanogaster embargoed (emb), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16476
Clone ID Related Accession (Same CDS sequence)	NM_164818.2 , NM_001316387.1
Accession Version	NM_001316387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	embargoed, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGACAA TGTTGACATC GGACGAGGCG GGCAAGCTGC TCGACTTCTC CCAGAAGCTG 
GACATTAATC TGCTGGACAA GATCGTCGAG GTTGTGTACA CAGCGCAGGG CGAGCAGTTG 
CGTCTAGCCC AGAGCATACT GACCACGCTG AAGGAGCATC CAGAGGCATG GACGCGGGTG 
GACAGCATAT TGGAGTACTC ACAGAACCAG CGGACCAAGT TTTATGCTCT GCAAATCCTC 
GAGGAGGTCA TCAAAACGCG CTGGAAGGTC CTGCCGCGCA ACCAGTGCGA GGGAATCAAG 
AAGTATGTGG TCAGTCTTAT CATCAAGACC TCGTCGGATC CCATTGTTAT GGAGCAGAAC 
AAAGTGTATC TGAATAAGCT AAACATGATT CTGGTCCACA TTCTAAAGCG CGAATGGCCA 
CGCAACTGGG AGACATTCAT CAGTGACATT GTCGGTGCGT CGAAAACGAA CGAGAGCCTG 
TGCATGAACA ACATGGTAAT CCTGAAGAAC CTTAGTGAGG AGGTGTTCGA CTTTTCGCAG 
GGTCAGATCA CGCAGACTAA GGCCAAGCAC CTGAAAGACA CCATGTGCTC GGAGTTCTCT 
CAGATCTTTA CACTATGCTC CTTCGTGCTG GAGAACTCGA TGAATGCCGC CCTAATCCAT 
GTCACGCTGG AGACTCTGCT GAGGTTCCTT AACTGGATAC CGCTGGGCTA CATCTTTGAA 
ACGCAGCAAA TTGAAACGCT GATATTCAAA TTCCTTAGCG TACCCATGTT CCGCAATGTG 
ACCCTGAAGT GTCTGTCCGA GATCGCCGGC CTGACTGCGG CAAACTACGA CGAGAATTTC 
GCGACGCTCT TCAAGGACAC GATGGTTCAG TTGGAACAGA TAGTCGGCCA GAATATGAAC 
ATGAACCATG TGTTTAAGCA TGGTTCCGAC ACAGAGCAGG AGCTGGTCCT TAATTTGGCC 
ATGTTCCTGT GCACCTTCCT CAAGGAGCAC GGCAAACTGG TGGAGGATGC CAAGTATGTG 
GACTACCTGA ATCAGGCGCT CATGTATTTG GTGATGATTA GCGAGGTGGA GGACGTGGAA 
GTATTCAAGA TCTGTCTGGA ATACTGGAAC AGCCTTGTGG AGGATCTATA CAATTCGGAA 
TTCTTCCACC CCACCCTGGA GTCTACAAAG AGGCAACAAG TGTATCCCCG TCGACGTTTC 
TACGCCCCCA TCCTGTCCAA AGTGCGATTC ATTATGATCT CGCGCATGGC CAAGCCGGAG 
GAGGTGCTGG TGGTGGAGAA CGAAAACGGG GAAGTGGTGC GCGAGTTCAT GAAGGACACA 
AACTCAATAA ACCTGTACAA GAACATGCGC GAAACGCTGG TCTTTCTTAC CCATTTGGAC 
TCTGTGGACA CGGACCGCAT CATGACGCTA AAGCTGCTTA ATCAGGTGAA CGGATCGGAA 
TTCTCTTGGA AGAACCTGAA CACGTTGTGC TGGGCCATTG GTTCCATATC CGGTGCTTTT 
TGTGAAGAGG ACGAAAAACG GTTCCTGGTC ACCGTGATCA AGGATCTATT AGGCCTGTGC 
GAACAGAAGA AGGGCAAGGA CAACAAGGCC ATTATTGCCT CCAATATCAT GTATGTGGTG 
GGACAGTATC CACGCTTCCT GCGCGCCCAT TGGAAGTTCC TCAAGACGGT GGTAAACAAA 
CTCTTCGAAT TTATGCACGA AACGCACGAT GGTGTCCAGG ATATGGCATG CGATACGTTC 
ATCAAGATAG CCATCAAATG CCGCCGCTAC TTTGTCACCA TCCAGCCGAA TGAGGCATGC 
ACCTTTATTG ACGAGATTCT CACAACAATG AGCAGCATCA TTTGTGATCT GCAGCCGCAG 
CAGGTTCACA CGTTCTATGA AGCTGTCGGC TACATGATCT CCGCCCAGGT GGATCAGGTA 
CAACAGGACG TCCTCATCGA GAGGTACATG CAACTGCCCA ACCAGGTGTG GGATGACATC 
ATCTCCCGCG CCTCGAAAAA CGTTGACTTC TTGAAGAACA TGACCGCTGT CAAGCAGCTG 
GGCAGCATCC TTAAAACGAA TGTGGCTGCC TGCAAAGCCC TGGGTCACGC GTATGTCATT 
CAACTGGGCC GCATATATCT GGACATGCTG AATGTGTACA AGATAACATC AGAGAATATC 
ATTCAGGCCA TCGAGGTGAA TGGCGTCAAT GTGAACAATC AGCCCCTGAT CAAAACCATG 
CATGTGGTCA AGAAGGAGAC GCTTAACTTG ATATCCGAGT GGGTGTCTCG TTCCAACGAC 
AATCAGTTGG TGATGGACAA TTTCATACCG CCTTTGCTGG ACGCTATACT GCTAGATTAT 
CAGCGCTGCA AAGTTCCATC GGCACGGGAG CCCAAGGTTT TAAGTGCGAT GGCAATCATT 
GTGCATAAAC TGCGTCAGCA TATCACAAAT GAAGTACCAA AAATATTTGA CGCCGTCTTC 
GAGTGCACGC TGGACATGAT CAACAAGAAC TTTGAAGATT TCCCACAGCA CAGACTTAGT 
TTCTACGAGC TACTGCAGGC GGTCAATGCG CACTGCTTTA AGGCGTTCCT TAATATACCG 
CCGGCTCAGT TCAAACTGGT CTTCGACTCG GTGGTGTGGG CATTCAAGCA TACCATGCGG 
AATGTGGCCG ACATGGGATT GAATATCCTA TTTAAGATGC TTCAAAACTT AGATCAGCAT 
CCCGGTGCGG CGCAGAGCTT CTATCAGACG TACTTTACAG ATATTCTAAT GCAGATATTT 
TCGGTGGTCA CTGACACCTC ACACACGGCC GGGTTGCCCA ATCATGCCAT CATTCTGGCG 
TACATGTTCT CACTGGTGGA GAACAGGAAG ATTACGGTGA ACCTGGGTCC GATTCCGGAT 
AATATGATAT TCATACAGGA ATATGTCGCG TCGCTGCTGA AATCGGCATT TACCCATCTG 
TCCGATAATC AGGTCAAAGT CTTTGTCACT GGCTTATTCA ATTTAGACGA GAATGTGCAA 
GCATTTAAGG AGCATTTGAG AGATTTTCTC ATACAGATTC GCGAGGCCAC CGGCGAGGAC 
GATTCCGATC TGTACTTGGA AGAGCGTGAA GCGGCTCTGG CCGAGGAGCA GTCAAATAAA 
CATCAGATGC AGCGTAATAT TCCAGGAATG TTGAATCCGC ACGAGTTGCC CGAGGATATG 
CAGGACGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303316.1
CDS	372..3563
Translation	MATMLTSDEAGKLLDFSQKLDINLLDKIVEVVYTAQGEQLRLAQSILTTLKEHPEAWTRVDSILEYSQNQRTKFYALQILEEVIKTRWKVLPRNQCEGIKKYVVSLIIKTSSDPIVMEQNKVYLNKLNMILVHILKREWPRNWETFISDIVGASKTNESLCMNNMVILKNLSEEVFDFSQGQITQTKAKHLKDTMCSEFSQIFTLCSFVLENSMNAALIHVTLETLLRFLNWIPLGYIFETQQIETLIFKFLSVPMFRNVTLKCLSEIAGLTAANYDENFATLFKDTMVQLEQIVGQNMNMNHVFKHGSDTEQELVLNLAMFLCTFLKEHGKLVEDAKYVDYLNQALMYLVMISEVEDVEVFKICLEYWNSLVEDLYNSEFFHPTLESTKRQQVYPRRRFYAPILSKVRFIMISRMAKPEEVLVVENENGEVVREFMKDTNSINLYKNMRETLVFLTHLDSVDTDRIMTLKLLNQVNGSEFSWKNLNTLCWAIGSISGAFCEEDEKRFLVTVIKDLLGLCEQKKGKDNKAIIASNIMYVVGQYPRFLRAHWKFLKTVVNKLFEFMHETHDGVQDMACDTFIKIAIKCRRYFVTIQPNEACTFIDEILTTMSSIICDLQPQQVHTFYEAVGYMISAQVDQVQQDVLIERYMQLPNQVWDDIISRASKNVDFLKNMTAVKQLGSILKTNVAACKALGHAYVIQLGRIYLDMLNVYKITSENIIQAIEVNGVNVNNQPLIKTMHVVKKETLNLISEWVSRSNDNQLVMDNFIPPLLDAILLDYQRCKVPSAREPKVLSAMAIIVHKLRQHITNEVPKIFDAVFECTLDMINKNFEDFPQHRLSFYELLQAVNAHCFKAFLNIPPAQFKLVFDSVVWAFKHTMRNVADMGLNILFKMLQNLDQHPGAAQSFYQTYFTDILMQIFSVVTDTSHTAGLPNHAIILAYMFSLVENRKITVNLGPIPDNMIFIQEYVASLLKSAFTHLSDNQVKVFVTGLFNLDENVQAFKEHLRDFLIQIREATGEDDSDLYLEEREAALAEEQSNKHQMQRNIPGMLNPHELPEDMQDE

Target ORF information:

RefSeq Version	NM_001316387.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster embargoed (emb), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316387.1

ORF Insert Sequence:

ATGGCGACAA TGTTGACATC GGACGAGGCG GGCAAGCTGC TCGACTTCTC CCAGAAGCTG 
GACATTAATC TGCTGGACAA GATCGTCGAG GTTGTGTACA CAGCGCAGGG CGAGCAGTTG 
CGTCTAGCCC AGAGCATACT GACCACGCTG AAGGAGCATC CAGAGGCATG GACGCGGGTG 
GACAGCATAT TGGAGTACTC ACAGAACCAG CGGACCAAGT TTTATGCTCT GCAAATCCTC 
GAGGAGGTCA TCAAAACGCG CTGGAAGGTC CTGCCGCGCA ACCAGTGCGA GGGAATCAAG 
AAGTATGTGG TCAGTCTTAT CATCAAGACC TCGTCGGATC CCATTGTTAT GGAGCAGAAC 
AAAGTGTATC TGAATAAGCT AAACATGATT CTGGTCCACA TTCTAAAGCG CGAATGGCCA 
CGCAACTGGG AGACATTCAT CAGTGACATT GTCGGTGCGT CGAAAACGAA CGAGAGCCTG 
TGCATGAACA ACATGGTAAT CCTGAAGAAC CTTAGTGAGG AGGTGTTCGA CTTTTCGCAG 
GGTCAGATCA CGCAGACTAA GGCCAAGCAC CTGAAAGACA CCATGTGCTC GGAGTTCTCT 
CAGATCTTTA CACTATGCTC CTTCGTGCTG GAGAACTCGA TGAATGCCGC CCTAATCCAT 
GTCACGCTGG AGACTCTGCT GAGGTTCCTT AACTGGATAC CGCTGGGCTA CATCTTTGAA 
ACGCAGCAAA TTGAAACGCT GATATTCAAA TTCCTTAGCG TACCCATGTT CCGCAATGTG 
ACCCTGAAGT GTCTGTCCGA GATCGCCGGC CTGACTGCGG CAAACTACGA CGAGAATTTC 
GCGACGCTCT TCAAGGACAC GATGGTTCAG TTGGAACAGA TAGTCGGCCA GAATATGAAC 
ATGAACCATG TGTTTAAGCA TGGTTCCGAC ACAGAGCAGG AGCTGGTCCT TAATTTGGCC 
ATGTTCCTGT GCACCTTCCT CAAGGAGCAC GGCAAACTGG TGGAGGATGC CAAGTATGTG 
GACTACCTGA ATCAGGCGCT CATGTATTTG GTGATGATTA GCGAGGTGGA GGACGTGGAA 
GTATTCAAGA TCTGTCTGGA ATACTGGAAC AGCCTTGTGG AGGATCTATA CAATTCGGAA 
TTCTTCCACC CCACCCTGGA GTCTACAAAG AGGCAACAAG TGTATCCCCG TCGACGTTTC 
TACGCCCCCA TCCTGTCCAA AGTGCGATTC ATTATGATCT CGCGCATGGC CAAGCCGGAG 
GAGGTGCTGG TGGTGGAGAA CGAAAACGGG GAAGTGGTGC GCGAGTTCAT GAAGGACACA 
AACTCAATAA ACCTGTACAA GAACATGCGC GAAACGCTGG TCTTTCTTAC CCATTTGGAC 
TCTGTGGACA CGGACCGCAT CATGACGCTA AAGCTGCTTA ATCAGGTGAA CGGATCGGAA 
TTCTCTTGGA AGAACCTGAA CACGTTGTGC TGGGCCATTG GTTCCATATC CGGTGCTTTT 
TGTGAAGAGG ACGAAAAACG GTTCCTGGTC ACCGTGATCA AGGATCTATT AGGCCTGTGC 
GAACAGAAGA AGGGCAAGGA CAACAAGGCC ATTATTGCCT CCAATATCAT GTATGTGGTG 
GGACAGTATC CACGCTTCCT GCGCGCCCAT TGGAAGTTCC TCAAGACGGT GGTAAACAAA 
CTCTTCGAAT TTATGCACGA AACGCACGAT GGTGTCCAGG ATATGGCATG CGATACGTTC 
ATCAAGATAG CCATCAAATG CCGCCGCTAC TTTGTCACCA TCCAGCCGAA TGAGGCATGC 
ACCTTTATTG ACGAGATTCT CACAACAATG AGCAGCATCA TTTGTGATCT GCAGCCGCAG 
CAGGTTCACA CGTTCTATGA AGCTGTCGGC TACATGATCT CCGCCCAGGT GGATCAGGTA 
CAACAGGACG TCCTCATCGA GAGGTACATG CAACTGCCCA ACCAGGTGTG GGATGACATC 
ATCTCCCGCG CCTCGAAAAA CGTTGACTTC TTGAAGAACA TGACCGCTGT CAAGCAGCTG 
GGCAGCATCC TTAAAACGAA TGTGGCTGCC TGCAAAGCCC TGGGTCACGC GTATGTCATT 
CAACTGGGCC GCATATATCT GGACATGCTG AATGTGTACA AGATAACATC AGAGAATATC 
ATTCAGGCCA TCGAGGTGAA TGGCGTCAAT GTGAACAATC AGCCCCTGAT CAAAACCATG 
CATGTGGTCA AGAAGGAGAC GCTTAACTTG ATATCCGAGT GGGTGTCTCG TTCCAACGAC 
AATCAGTTGG TGATGGACAA TTTCATACCG CCTTTGCTGG ACGCTATACT GCTAGATTAT 
CAGCGCTGCA AAGTTCCATC GGCACGGGAG CCCAAGGTTT TAAGTGCGAT GGCAATCATT 
GTGCATAAAC TGCGTCAGCA TATCACAAAT GAAGTACCAA AAATATTTGA CGCCGTCTTC 
GAGTGCACGC TGGACATGAT CAACAAGAAC TTTGAAGATT TCCCACAGCA CAGACTTAGT 
TTCTACGAGC TACTGCAGGC GGTCAATGCG CACTGCTTTA AGGCGTTCCT TAATATACCG 
CCGGCTCAGT TCAAACTGGT CTTCGACTCG GTGGTGTGGG CATTCAAGCA TACCATGCGG 
AATGTGGCCG ACATGGGATT GAATATCCTA TTTAAGATGC TTCAAAACTT AGATCAGCAT 
CCCGGTGCGG CGCAGAGCTT CTATCAGACG TACTTTACAG ATATTCTAAT GCAGATATTT 
TCGGTGGTCA CTGACACCTC ACACACGGCC GGGTTGCCCA ATCATGCCAT CATTCTGGCG 
TACATGTTCT CACTGGTGGA GAACAGGAAG ATTACGGTGA ACCTGGGTCC GATTCCGGAT 
AATATGATAT TCATACAGGA ATATGTCGCG TCGCTGCTGA AATCGGCATT TACCCATCTG 
TCCGATAATC AGGTCAAAGT CTTTGTCACT GGCTTATTCA ATTTAGACGA GAATGTGCAA 
GCATTTAAGG AGCATTTGAG AGATTTTCTC ATACAGATTC GCGAGGCCAC CGGCGAGGAC 
GATTCCGATC TGTACTTGGA AGAGCGTGAA GCGGCTCTGG CCGAGGAGCA GTCAAATAAA 
CATCAGATGC AGCGTAATAT TCCAGGAATG TTGAATCCGC ACGAGTTGCC CGAGGATATG 
CAGGACGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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emb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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