AlaRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	AlaRS
Entrez Gene ID	34156
Full Name	Alanyl-tRNA synthetase
Synonyms	ARS,Aats-ala,CG13391,Dmel\CG13391,alaS
General protein information	Preferred Names Alanyl-tRNA synthetase Names CG13391 gene product from transcript CG13391-RA AlaRS-PA AlaRS-PB CG13391-PA CG13391-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 29B3-29B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_078787.4	NP_523511.2	Alanyl-tRNA synthetase, isoform A
NM_205934.3	NP_995656.1	Alanyl-tRNA synthetase, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AARS	XP_001169520.2
Caenorhabditis elegans (roundworm)	aars-2	NP_491281.1
Neurospora crassa	NCU02566	XP_965697.2
Xenopus tropicalis (tropical clawed frog)	aars	XP_002931707.1
	AARS	XP_002808351.1
Mus musculus (house mouse)	Aars	NP_666329.2
Danio rerio (zebrafish)	aars	NP_001037775.1
Oryza sativa (rice)	Os10g0182000	NP_001064254.1
Canis lupus familiaris (dog)	AARS	XP_536788.2
Gallus gallus (chicken)	AARS	NP_001005836.1
Drosophila melanogaster (fruit fly)	Aats-ala	NP_523511.2
Kluyveromyces lactis	KLLA0F02431g	XP_455190.1
Schizosaccharomyces pombe (fission yeast)	ala1	NP_593640.1
Homo sapiens (human)	AARS	NP_001596.2
Bos taurus (cattle)	AARS	NP_001094545.1
Rattus norvegicus (Norway rat)	Aars	NP_001093987.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009701	XP_318757.4
Saccharomyces cerevisiae (baker's yeast)	ALA1	NP_014980.3
Eremothecium gossypii	AGOS_ADR363C	NP_984459.1
Magnaporthe oryzae (rice blast fungus)	MGG_03607	XP_003716291.1
Arabidopsis thaliana (thale cress)	ALATS	NP_001185186.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila melanogaster mitochondrial tRNA (Ala) contains a G:U base pair that has been translocated to the adjacent 2:71 position.This G2:U71 is the major determinant for identity of Dm mt tRNA(Ala).
Title: Positional recognition of a tRNA determinant dependent on a peptide insertion.

The following AlaRS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AlaRS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16468	NM_205934.3 Latest version!	Drosophila melanogaster Alanyl-tRNA synthetase (AlaRS), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16468	NM_078787.4 Latest version!	Drosophila melanogaster Alanyl-tRNA synthetase (AlaRS), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AlaRS ( NM_078787.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523511.2 Drosophila melanogaster Alanyl-tRNA synthetase (AlaRS), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16468
Clone ID Related Accession (Same CDS sequence)	NM_078787.4 , NM_205934.3
Accession Version	NM_078787.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Alanyl-tRNA synthetase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078787.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTAC TCACAGCAAA GGAGGTGCGC AACGCCTATC TGGACTTCTT CAAGGAGCAG 
AAGCACATCT ATGTTCACTC GTCCAGCACG ATTCCATTGG ACGATCCCAC GCTGTTGTTC 
GCCAACGCCG GCATGAATCA GTTCAAGCCC ATTTTTCTGG GCACCGCTGA TCCCAACAGC 
GAAATGTCCA AGTGGGTGCG CGTGGCCAAC ACGCAGAAGT GCATTCGCGC CGGTGGCAAG 
CACAACGATC TGGACGACGT GGGCAAGGAT GTCTATCATC ACACCTTCTT TGAAATGCTG 
GGAAACTGGT CCTTCGGTGA CTACTTTAAG AAGGAGATCT GCTCCTGGGC CTGGGAGTTT 
CTCACGCAGC GACTCGCCCT ACCCAAGGAT CGTCTGTATG TGACTTACTT TGGTGGTGAC 
GCGGCCAGCG GTCTGGAGCC CGATCTGGAG TGCAAGCAAA TGTGGCTGGA CTTGGGCCTG 
AAACCGGAAC ACATCCTGCC GGGCAGCATG AAGGACAACT TCTGGGAGAT GGGCGAGACT 
GGGCCCTGCG GACCATGCTC TGAGCTGCAC TTCGACCGGA TCGGCGGGCG CAGCGTTCCG 
GAGCTGGTCA ACATGGACGA TCCCGATGTC CTGGAGATCT GGAATCTCGT GTTCATCCAG 
TACAACCGTG AATCTGATGG CAGCTTGAAG CAACTTCCCA AGAAGCACAT TGATTGCGGC 
ATGGGTTTCG AGCGGCTGGT GTCCGTCATC CAGAATAAGC GATCTAACTA CGATACCGAT 
CTGTTTGTGC CACTTTTCGA TGCCATCCAG GCGGGCACTG GTGCCCCACC TTACCAGGGT 
CGTGTGGGTG CCGACGATGT GGACGGCATT GACATGGCCT ACCGCGTGCT TGCCGATCAC 
GCCCGCACCA TCACCATTGC TTTGGCTGAT GGCGGCACTC CGGACAACAC TGGCCGAGGC 
TATGTGCTGC GACGCATCCT TCGTCGCGCT GTGCGTTATG CCACTGAGAA GTTGAATGCC 
AAGCCCGGTT TCTTCGCCAC CCTGGTGAAC ACAGTGGTTG ATCTGCTTGG TGATGCCTTC 
CCGGAGGTGA AAAAAGATCC CCAACACATC ATTGATATTA TCAACGAGGA AGAGTTGCAG 
TTCCTTAAGA CGTTAACGCG CGGGCGCAAC TTGCTGAACC GCACAATCGA AAAGTTGGGT 
AACCAGACTA CCATTCCCGG AGATGTTGCT TGGCGGCTGT ACGACACCTA CGGCTTCCCA 
GTCGATCTAA CCCAGCTTAT GGCAGAGGAG AAATCCTTGA AAATCGATAT GGATGGCTAT 
GAGGCAGCCA AGCATAATTC ATATGTGCTT TCGCAGGGCA AAGGTGCTAG CAAAATCGAG 
GAGATCAATC TGGATGTGCA CGCCATTTCG CAGCTGCAGG AGCAAGGTGT GCCACCAACC 
AACGACACCT TCAAGTACAA GTACGAGGCT GTGTCTGACG AACGTGACTC CGCCTACAAC 
TACGGTGTGT GCAATAGTAA AATTGTTGCC CTGCGCTTCG AAAACCAGTT TGTGAACGAG 
ATCACCAGCG GACAGAAGGC GGGCATCGTT CTGGACAAAA CAAATTTCTA TGCAGAGAGC 
GGTGGTCAAA TCTACGACCA AGGTGCACTG GTTAAAGTCA ATGACGAGGC GAACGAGTTC 
CTTGTGGACC GTGTCTACAA CCGCGGAGGC TACATTCTTC ACATTGGCGT TGTAGAGGGC 
ACCCTTAAGG TGGGTGATGA GTTGGAACTG CACATCGACG TGGAGCGTCG CTGGTTGACC 
ATGAAAAACC ACTCGGCCAC CCATGCCCTC AACCATTGCT TGCTGCAAGT ATTGGGTAAG 
GACACCGAAC AGAAAGGCTC TCTGGTAGTG CCAGAGAAGC TACGCTTTGA CTTCAACAGC 
AAGGCGGCAA TGACCATCGA GCAGGTGTCC AAGACGGAGC AGCTGACTAA GGAGATGGTG 
TACAAGAATG TGCCGATCTA CGCAAAGGAG TCGAAATTGG CCCTGGCCAA GAAGATCCGA 
GGCCTGCGAT CGGTCTTCGA CGAAGTGTAC CCGGATCCCG TTAGGGTAAT CTCCTTCGGA 
GTGCCCGTAG ATGAACTGGA GCAGAATCCC GATTCTGAAG CCGGCGAACA AACCTCCGTC 
GAGTTTTGCG GCGGCACCCA TTTGAGGCGC TCGGGTCACA TAATGGACTT TGTTATCAGC 
AGCGAAGAGG CCATTGCCAA GGGTATCCGA AGAATTGTGG CTCTTACAGG ACCCGAAGCT 
CTGAAGGCAC TGAAGAAATC TGAAGCATTT GAACAAGAAA TCGTCCGGCT TAAGGCCACC 
ATTGATAACG ATAAGTCCGG TAAGGACTCC AAGTCGCATG TGAAGGAGAT TGTTGAGCTG 
ACTGAACAGA TCTCGCATGC TACGATTCCC TACGTGAAAA AGGACGAGAT GCGCAATCTG 
CTGAAGGGCT TGAAGAAAAC ATTGGACGAT AAGGAACGTG CCCTGCGTGC CGCTGTCTCG 
GTCACTGTTG TGGAGCGCGC CAAGACTCTG TGCGAAGCCA ATCCCAATGC CACCGTGCTG 
GTCGAGCAGT TGGAAGCCTT TAACAACACC AAGGCGCTGG ATGCCGCACT GAAGCAAGTG 
CGCAGCCAGC TGCCCGACGC TGCCGCCATG TTTCTGTCAG TGGATGCGGA CTCCAAGAAG 
ATCTTCTGTC TCAGCTCGGT GCCGAAAAGT GCCGTGGAAA AGGGTCTGAA GGCCAACGAG 
TGGGTGCAGC ATGTTTCCGC CACGCTGGGA GGCAAGGGAG GTGGCAAACC GGAATCCGCC 
CAGGCATCGG GCACAAATTA CGAAAAGGTG GACGAAATTG TCCAATTGGC CAGCAAATTC 
GCTCAGTCGA AACTGTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523511.2
CDS	96..2996
Translation	MKLLTAKEVRNAYLDFFKEQKHIYVHSSSTIPLDDPTLLFANAGMNQFKPIFLGTADPNSEMSKWVRVANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICSWAWEFLTQRLALPKDRLYVTYFGGDAASGLEPDLECKQMWLDLGLKPEHILPGSMKDNFWEMGETGPCGPCSELHFDRIGGRSVPELVNMDDPDVLEIWNLVFIQYNRESDGSLKQLPKKHIDCGMGFERLVSVIQNKRSNYDTDLFVPLFDAIQAGTGAPPYQGRVGADDVDGIDMAYRVLADHARTITIALADGGTPDNTGRGYVLRRILRRAVRYATEKLNAKPGFFATLVNTVVDLLGDAFPEVKKDPQHIIDIINEEELQFLKTLTRGRNLLNRTIEKLGNQTTIPGDVAWRLYDTYGFPVDLTQLMAEEKSLKIDMDGYEAAKHNSYVLSQGKGASKIEEINLDVHAISQLQEQGVPPTNDTFKYKYEAVSDERDSAYNYGVCNSKIVALRFENQFVNEITSGQKAGIVLDKTNFYAESGGQIYDQGALVKVNDEANEFLVDRVYNRGGYILHIGVVEGTLKVGDELELHIDVERRWLTMKNHSATHALNHCLLQVLGKDTEQKGSLVVPEKLRFDFNSKAAMTIEQVSKTEQLTKEMVYKNVPIYAKESKLALAKKIRGLRSVFDEVYPDPVRVISFGVPVDELEQNPDSEAGEQTSVEFCGGTHLRRSGHIMDFVISSEEAIAKGIRRIVALTGPEALKALKKSEAFEQEIVRLKATIDNDKSGKDSKSHVKEIVELTEQISHATIPYVKKDEMRNLLKGLKKTLDDKERALRAAVSVTVVERAKTLCEANPNATVLVEQLEAFNNTKALDAALKQVRSQLPDAAAMFLSVDADSKKIFCLSSVPKSAVEKGLKANEWVQHVSATLGGKGGGKPESAQASGTNYEKVDEIVQLASKFAQSKLS

Target ORF information:

RefSeq Version	NM_078787.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Alanyl-tRNA synthetase (AlaRS), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078787.4

ORF Insert Sequence:

ATGAAGTTAC TCACAGCAAA GGAGGTGCGC AACGCCTATC TGGACTTCTT CAAGGAGCAG 
AAGCACATCT ATGTTCACTC GTCCAGCACG ATTCCATTGG ACGATCCCAC GCTGTTGTTC 
GCCAACGCCG GCATGAATCA GTTCAAGCCC ATTTTTCTGG GCACCGCTGA TCCCAACAGC 
GAAATGTCCA AGTGGGTGCG CGTGGCCAAC ACGCAGAAGT GCATTCGCGC CGGTGGCAAG 
CACAACGATC TGGACGACGT GGGCAAGGAT GTCTATCATC ACACCTTCTT TGAAATGCTG 
GGAAACTGGT CCTTCGGTGA CTACTTTAAG AAGGAGATCT GCTCCTGGGC CTGGGAGTTT 
CTCACGCAGC GACTCGCCCT ACCCAAGGAT CGTCTGTATG TGACTTACTT TGGTGGTGAC 
GCGGCCAGCG GTCTGGAGCC CGATCTGGAG TGCAAGCAAA TGTGGCTGGA CTTGGGCCTG 
AAACCGGAAC ACATCCTGCC GGGCAGCATG AAGGACAACT TCTGGGAGAT GGGCGAGACT 
GGGCCCTGCG GACCATGCTC TGAGCTGCAC TTCGACCGGA TCGGCGGGCG CAGCGTTCCG 
GAGCTGGTCA ACATGGACGA TCCCGATGTC CTGGAGATCT GGAATCTCGT GTTCATCCAG 
TACAACCGTG AATCTGATGG CAGCTTGAAG CAACTTCCCA AGAAGCACAT TGATTGCGGC 
ATGGGTTTCG AGCGGCTGGT GTCCGTCATC CAGAATAAGC GATCTAACTA CGATACCGAT 
CTGTTTGTGC CACTTTTCGA TGCCATCCAG GCGGGCACTG GTGCCCCACC TTACCAGGGT 
CGTGTGGGTG CCGACGATGT GGACGGCATT GACATGGCCT ACCGCGTGCT TGCCGATCAC 
GCCCGCACCA TCACCATTGC TTTGGCTGAT GGCGGCACTC CGGACAACAC TGGCCGAGGC 
TATGTGCTGC GACGCATCCT TCGTCGCGCT GTGCGTTATG CCACTGAGAA GTTGAATGCC 
AAGCCCGGTT TCTTCGCCAC CCTGGTGAAC ACAGTGGTTG ATCTGCTTGG TGATGCCTTC 
CCGGAGGTGA AAAAAGATCC CCAACACATC ATTGATATTA TCAACGAGGA AGAGTTGCAG 
TTCCTTAAGA CGTTAACGCG CGGGCGCAAC TTGCTGAACC GCACAATCGA AAAGTTGGGT 
AACCAGACTA CCATTCCCGG AGATGTTGCT TGGCGGCTGT ACGACACCTA CGGCTTCCCA 
GTCGATCTAA CCCAGCTTAT GGCAGAGGAG AAATCCTTGA AAATCGATAT GGATGGCTAT 
GAGGCAGCCA AGCATAATTC ATATGTGCTT TCGCAGGGCA AAGGTGCTAG CAAAATCGAG 
GAGATCAATC TGGATGTGCA CGCCATTTCG CAGCTGCAGG AGCAAGGTGT GCCACCAACC 
AACGACACCT TCAAGTACAA GTACGAGGCT GTGTCTGACG AACGTGACTC CGCCTACAAC 
TACGGTGTGT GCAATAGTAA AATTGTTGCC CTGCGCTTCG AAAACCAGTT TGTGAACGAG 
ATCACCAGCG GACAGAAGGC GGGCATCGTT CTGGACAAAA CAAATTTCTA TGCAGAGAGC 
GGTGGTCAAA TCTACGACCA AGGTGCACTG GTTAAAGTCA ATGACGAGGC GAACGAGTTC 
CTTGTGGACC GTGTCTACAA CCGCGGAGGC TACATTCTTC ACATTGGCGT TGTAGAGGGC 
ACCCTTAAGG TGGGTGATGA GTTGGAACTG CACATCGACG TGGAGCGTCG CTGGTTGACC 
ATGAAAAACC ACTCGGCCAC CCATGCCCTC AACCATTGCT TGCTGCAAGT ATTGGGTAAG 
GACACCGAAC AGAAAGGCTC TCTGGTAGTG CCAGAGAAGC TACGCTTTGA CTTCAACAGC 
AAGGCGGCAA TGACCATCGA GCAGGTGTCC AAGACGGAGC AGCTGACTAA GGAGATGGTG 
TACAAGAATG TGCCGATCTA CGCAAAGGAG TCGAAATTGG CCCTGGCCAA GAAGATCCGA 
GGCCTGCGAT CGGTCTTCGA CGAAGTGTAC CCGGATCCCG TTAGGGTAAT CTCCTTCGGA 
GTGCCCGTAG ATGAACTGGA GCAGAATCCC GATTCTGAAG CCGGCGAACA AACCTCCGTC 
GAGTTTTGCG GCGGCACCCA TTTGAGGCGC TCGGGTCACA TAATGGACTT TGTTATCAGC 
AGCGAAGAGG CCATTGCCAA GGGTATCCGA AGAATTGTGG CTCTTACAGG ACCCGAAGCT 
CTGAAGGCAC TGAAGAAATC TGAAGCATTT GAACAAGAAA TCGTCCGGCT TAAGGCCACC 
ATTGATAACG ATAAGTCCGG TAAGGACTCC AAGTCGCATG TGAAGGAGAT TGTTGAGCTG 
ACTGAACAGA TCTCGCATGC TACGATTCCC TACGTGAAAA AGGACGAGAT GCGCAATCTG 
CTGAAGGGCT TGAAGAAAAC ATTGGACGAT AAGGAACGTG CCCTGCGTGC CGCTGTCTCG 
GTCACTGTTG TGGAGCGCGC CAAGACTCTG TGCGAAGCCA ATCCCAATGC CACCGTGCTG 
GTCGAGCAGT TGGAAGCCTT TAACAACACC AAGGCGCTGG ATGCCGCACT GAAGCAAGTG 
CGCAGCCAGC TGCCCGACGC TGCCGCCATG TTTCTGTCAG TGGATGCGGA CTCCAAGAAG 
ATCTTCTGTC TCAGCTCGGT GCCGAAAAGT GCCGTGGAAA AGGGTCTGAA GGCCAACGAG 
TGGGTGCAGC ATGTTTCCGC CACGCTGGGA GGCAAGGGAG GTGGCAAACC GGAATCCGCC 
CAGGCATCGG GCACAAATTA CGAAAAGGTG GACGAAATTG TCCAATTGGC CAGCAAATTC 
GCTCAGTCGA AACTGTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AlaRS ( NM_205934.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995656.1 Drosophila melanogaster Alanyl-tRNA synthetase (AlaRS), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16468
Clone ID Related Accession (Same CDS sequence)	NM_078787.4 , NM_205934.3
Accession Version	NM_205934.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Alanyl-tRNA synthetase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_205934.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTTAC TCACAGCAAA GGAGGTGCGC AACGCCTATC TGGACTTCTT CAAGGAGCAG 
AAGCACATCT ATGTTCACTC GTCCAGCACG ATTCCATTGG ACGATCCCAC GCTGTTGTTC 
GCCAACGCCG GCATGAATCA GTTCAAGCCC ATTTTTCTGG GCACCGCTGA TCCCAACAGC 
GAAATGTCCA AGTGGGTGCG CGTGGCCAAC ACGCAGAAGT GCATTCGCGC CGGTGGCAAG 
CACAACGATC TGGACGACGT GGGCAAGGAT GTCTATCATC ACACCTTCTT TGAAATGCTG 
GGAAACTGGT CCTTCGGTGA CTACTTTAAG AAGGAGATCT GCTCCTGGGC CTGGGAGTTT 
CTCACGCAGC GACTCGCCCT ACCCAAGGAT CGTCTGTATG TGACTTACTT TGGTGGTGAC 
GCGGCCAGCG GTCTGGAGCC CGATCTGGAG TGCAAGCAAA TGTGGCTGGA CTTGGGCCTG 
AAACCGGAAC ACATCCTGCC GGGCAGCATG AAGGACAACT TCTGGGAGAT GGGCGAGACT 
GGGCCCTGCG GACCATGCTC TGAGCTGCAC TTCGACCGGA TCGGCGGGCG CAGCGTTCCG 
GAGCTGGTCA ACATGGACGA TCCCGATGTC CTGGAGATCT GGAATCTCGT GTTCATCCAG 
TACAACCGTG AATCTGATGG CAGCTTGAAG CAACTTCCCA AGAAGCACAT TGATTGCGGC 
ATGGGTTTCG AGCGGCTGGT GTCCGTCATC CAGAATAAGC GATCTAACTA CGATACCGAT 
CTGTTTGTGC CACTTTTCGA TGCCATCCAG GCGGGCACTG GTGCCCCACC TTACCAGGGT 
CGTGTGGGTG CCGACGATGT GGACGGCATT GACATGGCCT ACCGCGTGCT TGCCGATCAC 
GCCCGCACCA TCACCATTGC TTTGGCTGAT GGCGGCACTC CGGACAACAC TGGCCGAGGC 
TATGTGCTGC GACGCATCCT TCGTCGCGCT GTGCGTTATG CCACTGAGAA GTTGAATGCC 
AAGCCCGGTT TCTTCGCCAC CCTGGTGAAC ACAGTGGTTG ATCTGCTTGG TGATGCCTTC 
CCGGAGGTGA AAAAAGATCC CCAACACATC ATTGATATTA TCAACGAGGA AGAGTTGCAG 
TTCCTTAAGA CGTTAACGCG CGGGCGCAAC TTGCTGAACC GCACAATCGA AAAGTTGGGT 
AACCAGACTA CCATTCCCGG AGATGTTGCT TGGCGGCTGT ACGACACCTA CGGCTTCCCA 
GTCGATCTAA CCCAGCTTAT GGCAGAGGAG AAATCCTTGA AAATCGATAT GGATGGCTAT 
GAGGCAGCCA AGCATAATTC ATATGTGCTT TCGCAGGGCA AAGGTGCTAG CAAAATCGAG 
GAGATCAATC TGGATGTGCA CGCCATTTCG CAGCTGCAGG AGCAAGGTGT GCCACCAACC 
AACGACACCT TCAAGTACAA GTACGAGGCT GTGTCTGACG AACGTGACTC CGCCTACAAC 
TACGGTGTGT GCAATAGTAA AATTGTTGCC CTGCGCTTCG AAAACCAGTT TGTGAACGAG 
ATCACCAGCG GACAGAAGGC GGGCATCGTT CTGGACAAAA CAAATTTCTA TGCAGAGAGC 
GGTGGTCAAA TCTACGACCA AGGTGCACTG GTTAAAGTCA ATGACGAGGC GAACGAGTTC 
CTTGTGGACC GTGTCTACAA CCGCGGAGGC TACATTCTTC ACATTGGCGT TGTAGAGGGC 
ACCCTTAAGG TGGGTGATGA GTTGGAACTG CACATCGACG TGGAGCGTCG CTGGTTGACC 
ATGAAAAACC ACTCGGCCAC CCATGCCCTC AACCATTGCT TGCTGCAAGT ATTGGGTAAG 
GACACCGAAC AGAAAGGCTC TCTGGTAGTG CCAGAGAAGC TACGCTTTGA CTTCAACAGC 
AAGGCGGCAA TGACCATCGA GCAGGTGTCC AAGACGGAGC AGCTGACTAA GGAGATGGTG 
TACAAGAATG TGCCGATCTA CGCAAAGGAG TCGAAATTGG CCCTGGCCAA GAAGATCCGA 
GGCCTGCGAT CGGTCTTCGA CGAAGTGTAC CCGGATCCCG TTAGGGTAAT CTCCTTCGGA 
GTGCCCGTAG ATGAACTGGA GCAGAATCCC GATTCTGAAG CCGGCGAACA AACCTCCGTC 
GAGTTTTGCG GCGGCACCCA TTTGAGGCGC TCGGGTCACA TAATGGACTT TGTTATCAGC 
AGCGAAGAGG CCATTGCCAA GGGTATCCGA AGAATTGTGG CTCTTACAGG ACCCGAAGCT 
CTGAAGGCAC TGAAGAAATC TGAAGCATTT GAACAAGAAA TCGTCCGGCT TAAGGCCACC 
ATTGATAACG ATAAGTCCGG TAAGGACTCC AAGTCGCATG TGAAGGAGAT TGTTGAGCTG 
ACTGAACAGA TCTCGCATGC TACGATTCCC TACGTGAAAA AGGACGAGAT GCGCAATCTG 
CTGAAGGGCT TGAAGAAAAC ATTGGACGAT AAGGAACGTG CCCTGCGTGC CGCTGTCTCG 
GTCACTGTTG TGGAGCGCGC CAAGACTCTG TGCGAAGCCA ATCCCAATGC CACCGTGCTG 
GTCGAGCAGT TGGAAGCCTT TAACAACACC AAGGCGCTGG ATGCCGCACT GAAGCAAGTG 
CGCAGCCAGC TGCCCGACGC TGCCGCCATG TTTCTGTCAG TGGATGCGGA CTCCAAGAAG 
ATCTTCTGTC TCAGCTCGGT GCCGAAAAGT GCCGTGGAAA AGGGTCTGAA GGCCAACGAG 
TGGGTGCAGC ATGTTTCCGC CACGCTGGGA GGCAAGGGAG GTGGCAAACC GGAATCCGCC 
CAGGCATCGG GCACAAATTA CGAAAAGGTG GACGAAATTG TCCAATTGGC CAGCAAATTC 
GCTCAGTCGA AACTGTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995656.1
CDS	147..3047
Translation	MKLLTAKEVRNAYLDFFKEQKHIYVHSSSTIPLDDPTLLFANAGMNQFKPIFLGTADPNSEMSKWVRVANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICSWAWEFLTQRLALPKDRLYVTYFGGDAASGLEPDLECKQMWLDLGLKPEHILPGSMKDNFWEMGETGPCGPCSELHFDRIGGRSVPELVNMDDPDVLEIWNLVFIQYNRESDGSLKQLPKKHIDCGMGFERLVSVIQNKRSNYDTDLFVPLFDAIQAGTGAPPYQGRVGADDVDGIDMAYRVLADHARTITIALADGGTPDNTGRGYVLRRILRRAVRYATEKLNAKPGFFATLVNTVVDLLGDAFPEVKKDPQHIIDIINEEELQFLKTLTRGRNLLNRTIEKLGNQTTIPGDVAWRLYDTYGFPVDLTQLMAEEKSLKIDMDGYEAAKHNSYVLSQGKGASKIEEINLDVHAISQLQEQGVPPTNDTFKYKYEAVSDERDSAYNYGVCNSKIVALRFENQFVNEITSGQKAGIVLDKTNFYAESGGQIYDQGALVKVNDEANEFLVDRVYNRGGYILHIGVVEGTLKVGDELELHIDVERRWLTMKNHSATHALNHCLLQVLGKDTEQKGSLVVPEKLRFDFNSKAAMTIEQVSKTEQLTKEMVYKNVPIYAKESKLALAKKIRGLRSVFDEVYPDPVRVISFGVPVDELEQNPDSEAGEQTSVEFCGGTHLRRSGHIMDFVISSEEAIAKGIRRIVALTGPEALKALKKSEAFEQEIVRLKATIDNDKSGKDSKSHVKEIVELTEQISHATIPYVKKDEMRNLLKGLKKTLDDKERALRAAVSVTVVERAKTLCEANPNATVLVEQLEAFNNTKALDAALKQVRSQLPDAAAMFLSVDADSKKIFCLSSVPKSAVEKGLKANEWVQHVSATLGGKGGGKPESAQASGTNYEKVDEIVQLASKFAQSKLS

Target ORF information:

RefSeq Version	NM_205934.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Alanyl-tRNA synthetase (AlaRS), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205934.3

ORF Insert Sequence:

ATGAAGTTAC TCACAGCAAA GGAGGTGCGC AACGCCTATC TGGACTTCTT CAAGGAGCAG 
AAGCACATCT ATGTTCACTC GTCCAGCACG ATTCCATTGG ACGATCCCAC GCTGTTGTTC 
GCCAACGCCG GCATGAATCA GTTCAAGCCC ATTTTTCTGG GCACCGCTGA TCCCAACAGC 
GAAATGTCCA AGTGGGTGCG CGTGGCCAAC ACGCAGAAGT GCATTCGCGC CGGTGGCAAG 
CACAACGATC TGGACGACGT GGGCAAGGAT GTCTATCATC ACACCTTCTT TGAAATGCTG 
GGAAACTGGT CCTTCGGTGA CTACTTTAAG AAGGAGATCT GCTCCTGGGC CTGGGAGTTT 
CTCACGCAGC GACTCGCCCT ACCCAAGGAT CGTCTGTATG TGACTTACTT TGGTGGTGAC 
GCGGCCAGCG GTCTGGAGCC CGATCTGGAG TGCAAGCAAA TGTGGCTGGA CTTGGGCCTG 
AAACCGGAAC ACATCCTGCC GGGCAGCATG AAGGACAACT TCTGGGAGAT GGGCGAGACT 
GGGCCCTGCG GACCATGCTC TGAGCTGCAC TTCGACCGGA TCGGCGGGCG CAGCGTTCCG 
GAGCTGGTCA ACATGGACGA TCCCGATGTC CTGGAGATCT GGAATCTCGT GTTCATCCAG 
TACAACCGTG AATCTGATGG CAGCTTGAAG CAACTTCCCA AGAAGCACAT TGATTGCGGC 
ATGGGTTTCG AGCGGCTGGT GTCCGTCATC CAGAATAAGC GATCTAACTA CGATACCGAT 
CTGTTTGTGC CACTTTTCGA TGCCATCCAG GCGGGCACTG GTGCCCCACC TTACCAGGGT 
CGTGTGGGTG CCGACGATGT GGACGGCATT GACATGGCCT ACCGCGTGCT TGCCGATCAC 
GCCCGCACCA TCACCATTGC TTTGGCTGAT GGCGGCACTC CGGACAACAC TGGCCGAGGC 
TATGTGCTGC GACGCATCCT TCGTCGCGCT GTGCGTTATG CCACTGAGAA GTTGAATGCC 
AAGCCCGGTT TCTTCGCCAC CCTGGTGAAC ACAGTGGTTG ATCTGCTTGG TGATGCCTTC 
CCGGAGGTGA AAAAAGATCC CCAACACATC ATTGATATTA TCAACGAGGA AGAGTTGCAG 
TTCCTTAAGA CGTTAACGCG CGGGCGCAAC TTGCTGAACC GCACAATCGA AAAGTTGGGT 
AACCAGACTA CCATTCCCGG AGATGTTGCT TGGCGGCTGT ACGACACCTA CGGCTTCCCA 
GTCGATCTAA CCCAGCTTAT GGCAGAGGAG AAATCCTTGA AAATCGATAT GGATGGCTAT 
GAGGCAGCCA AGCATAATTC ATATGTGCTT TCGCAGGGCA AAGGTGCTAG CAAAATCGAG 
GAGATCAATC TGGATGTGCA CGCCATTTCG CAGCTGCAGG AGCAAGGTGT GCCACCAACC 
AACGACACCT TCAAGTACAA GTACGAGGCT GTGTCTGACG AACGTGACTC CGCCTACAAC 
TACGGTGTGT GCAATAGTAA AATTGTTGCC CTGCGCTTCG AAAACCAGTT TGTGAACGAG 
ATCACCAGCG GACAGAAGGC GGGCATCGTT CTGGACAAAA CAAATTTCTA TGCAGAGAGC 
GGTGGTCAAA TCTACGACCA AGGTGCACTG GTTAAAGTCA ATGACGAGGC GAACGAGTTC 
CTTGTGGACC GTGTCTACAA CCGCGGAGGC TACATTCTTC ACATTGGCGT TGTAGAGGGC 
ACCCTTAAGG TGGGTGATGA GTTGGAACTG CACATCGACG TGGAGCGTCG CTGGTTGACC 
ATGAAAAACC ACTCGGCCAC CCATGCCCTC AACCATTGCT TGCTGCAAGT ATTGGGTAAG 
GACACCGAAC AGAAAGGCTC TCTGGTAGTG CCAGAGAAGC TACGCTTTGA CTTCAACAGC 
AAGGCGGCAA TGACCATCGA GCAGGTGTCC AAGACGGAGC AGCTGACTAA GGAGATGGTG 
TACAAGAATG TGCCGATCTA CGCAAAGGAG TCGAAATTGG CCCTGGCCAA GAAGATCCGA 
GGCCTGCGAT CGGTCTTCGA CGAAGTGTAC CCGGATCCCG TTAGGGTAAT CTCCTTCGGA 
GTGCCCGTAG ATGAACTGGA GCAGAATCCC GATTCTGAAG CCGGCGAACA AACCTCCGTC 
GAGTTTTGCG GCGGCACCCA TTTGAGGCGC TCGGGTCACA TAATGGACTT TGTTATCAGC 
AGCGAAGAGG CCATTGCCAA GGGTATCCGA AGAATTGTGG CTCTTACAGG ACCCGAAGCT 
CTGAAGGCAC TGAAGAAATC TGAAGCATTT GAACAAGAAA TCGTCCGGCT TAAGGCCACC 
ATTGATAACG ATAAGTCCGG TAAGGACTCC AAGTCGCATG TGAAGGAGAT TGTTGAGCTG 
ACTGAACAGA TCTCGCATGC TACGATTCCC TACGTGAAAA AGGACGAGAT GCGCAATCTG 
CTGAAGGGCT TGAAGAAAAC ATTGGACGAT AAGGAACGTG CCCTGCGTGC CGCTGTCTCG 
GTCACTGTTG TGGAGCGCGC CAAGACTCTG TGCGAAGCCA ATCCCAATGC CACCGTGCTG 
GTCGAGCAGT TGGAAGCCTT TAACAACACC AAGGCGCTGG ATGCCGCACT GAAGCAAGTG 
CGCAGCCAGC TGCCCGACGC TGCCGCCATG TTTCTGTCAG TGGATGCGGA CTCCAAGAAG 
ATCTTCTGTC TCAGCTCGGT GCCGAAAAGT GCCGTGGAAA AGGGTCTGAA GGCCAACGAG 
TGGGTGCAGC ATGTTTCCGC CACGCTGGGA GGCAAGGGAG GTGGCAAACC GGAATCCGCC 
CAGGCATCGG GCACAAATTA CGAAAAGGTG GACGAAATTG TCCAATTGGC CAGCAAATTC 
GCTCAGTCGA AACTGTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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AlaRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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