Btk29A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Btk29A
Entrez Gene ID	34132
Full Name	Btk family kinase at 29A
Synonyms	Btk,Btk29,Btk29a,C-src2,C-src4,CG18355,CG8049,CT2415,CT41718,DSrc28,DTec29,Dm SRC2,Dmel\CG8049,Dsrc28C,Dsrc29A,EC2-9,S13,SRC 29A,Src2,Src29A,Tec,Tec29,Tec29A,btk,btk29,btk29A,btk29a,c-src/fps,dsrc29A,fic,src-4,src2,src28C,src4,tec29
General protein information	Preferred Names Btk family kinase at 29A Names CG8049 gene product from transcript CG8049-RE Btk29A type 2 Btk29A-PA Btk29A-PB Btk29A-PC Btk29A-PD Btk29A-PE Btk29A-PF Btk29A-PG Btk29A-PH CG8049-PA CG8049-PB CG8049-PC CG8049-PD CG8049-PE CG8049-PF CG8049-PG CG8049-PH Src oncogene 29A fickle
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 29A1-29A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_164804.2	NP_723369.1	Btk family kinase at 29A, isoform D
NM_001103650.2	NP_001097120.1	Btk family kinase at 29A, isoform F
NM_057398.4	NP_476746.1	Btk family kinase at 29A, isoform A
NM_001103651.3	NP_001097121.1	Btk family kinase at 29A, isoform E
NM_001273314.1	NP_001260243.1	Btk family kinase at 29A, isoform G
NM_057397.6	NP_476745.1	Btk family kinase at 29A, isoform B
NM_001273315.1	NP_001260244.1	Btk family kinase at 29A, isoform H
NM_164805.2	NP_723370.1	Btk family kinase at 29A, isoform C

Pathways from BioSystems

Homologies

	TEC	XP_001103213.1
Rattus norvegicus (Norway rat)	Tec	NP_445884.1
Gallus gallus (chicken)	TEC	NP_001025543.1
Bos taurus (cattle)	TEC	XP_005208032.1
Danio rerio (zebrafish)	tec	NP_001108215.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008317	XP_317149.4
Xenopus tropicalis (tropical clawed frog)	tec	XP_002933505.2
Canis lupus familiaris (dog)	TEC	XP_539259.2
Mus musculus (house mouse)	Tec	NP_001106931.1
Drosophila melanogaster (fruit fly)	Btk29A	NP_001097121.1
Homo sapiens (human)	TEC	NP_003206.2
Pan troglodytes (chimpanzee)	TEC	XP_517310.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Btk29A is required for the establishment of egg chamber polarity presumably through the regulation of subcellular localization of its downstream proteins, including Cno.
Title: Ovarian polarity and cell shape determination by Btk29A in Drosophila.

Btk29A(ficP) mutant males null for the Btk29A type 2 isoform, a fly homolog of the nonreceptor tyrosine kinase Btk, show no courtship suppression, while Btk29A hypomorphic males exhibit a complete loss in courtship suppression within 30 min.
Title: REQUIREMENT OF THE TEC FAMILY TYROSINE KINASE BTK29A FOR COURTSHIP MEMORY IN Drosophila MALES.

dissociation of tyrosine-phosphorylated Arm from DE-cadherin allows dynamic actin to reorganize, leading to ring canal expansion and cell shape changes during the course of oogenesis
Title: Btk29A-mediated tyrosine phosphorylation of armadillo/?-catenin promotes ring canal growth in Drosophila oogenesis.

Btk29A is required non cell-autonomously for the invagination of the spiracular chamber in Drosophila
Title: Btk-dependent epithelial cell rearrangements contribute to the invagination of nearby tubular structures in the posterior spiracles of Drosophila.

this study identifies a transcriptional switch involving the kinase Btk29A/Btk and its phosphorylation target, beta-catenin, which functions downstream of Wnt4 in escort cells to terminate Drosophila germ cell proliferation through up-regulation of piwi expression.
Title: Btk29A promotes Wnt4 signaling in the niche to terminate germ cell proliferation in Drosophila.

The following Btk29A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Btk29A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14117	NM_001273315.1 Latest version!	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa14118	NM_001273314.1 Latest version!	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16469	NM_001103650.2 Latest version!	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16469	NM_164805.2 Latest version!	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16469	NM_057398.4 Latest version!	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16445	NM_164804.2 Latest version!	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16445	NM_057397.6 Latest version!	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16445	NM_001103651.3 Latest version!	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Btk29A ( NM_001273315.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260244.1 Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa14117
Clone ID Related Accession (Same CDS sequence)	NM_001273315.1
Accession Version	NM_001273315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Btk family kinase at 29A, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTGGCG GTATACCCAC TCCCGGAACA CCAAATTCCA AAGCCAAGGA CAACTCCCAC 
TTTGTCAAGC TGGTGGTGGC TCTCTATCCA TTCAAGGCCA TCGAAGGCGG CGATTTATCG 
CTGGAAAAGA ATGCCGAATA CGAAGTGATA GATGACTCAC AAGAGCACTG GTGGAAGGTC 
AAAGATGCCT TGGGGAATGT CGGCTATATA CCCAGCAACT ATGTCAAGCC GAAGGCTCTT 
CTGGGCCTGG AGCGCTATGA ATGGTATGTG GGCGACATGT CACGACAGAG AGCCGAATCG 
CTGCTGAAGC AAGGTGATAA GGAGGGCTGC TTTGTGGTCA GGAAGTCATC GACCAAGGGT 
CTCTACACAC TATCGCTGCA TACCAAAGTT CCACAGTCGC ATGTGAAGCA CTACCACATC 
AAGCAAAATG CTCGCTGCGA GTATTATTTG AGTGAGAAGC ACTGCTGTGA AACAATTCCG 
GATCTGATTA ACTACCATCG CCACAACTCT GGGGGTTTGG CTTGCCGACT CAAATCCTCG 
CCCTGCGATC GCCCAGTTCC ACCGACGGCG GGATTGTCCC ACGACAAATG GGAGATCCAT 
CCGATGGAAC TGATGCTGAT GGAGGAGCTT GGATCGGGAC AGTTTGGTGT TGTGCGGCGC 
GGCAAGTGGC GTGGGTCTAT CGACACAGCG GTCAAGATGA TGAAGGAAGG AACCATGTCC 
GAGGACGATT TCATTGAGGA GGCCAAGGTG ATGACCAAGC TGCAGCATCC AAATCTTGTG 
CAGCTATATG GCGTCTGCTC CAAGCACCGG CCCATCTACA TTGTGACCGA GTACATGAAG 
CACGGATCCT TGTTGAATTA CTTGCGACGG CATGAGAAGA CCCTGATTGG TAATATGGGT 
CTACTCCTTG ACATGTGCAT ACAGGTTAGC AAGGGAATGA CCTACCTGGA GCGCCATAAC 
TACATTCACC GGGATCTGGC TGCCCGCAAC TGTCTCGTGG GTTCCGAGAA TGTCGTTAAA 
GTGGCCGACT TTGGATTGGC CCGATACGTT CTCGACGATC AGTATACCAG CTCTGGCGGA 
ACCAAGTTCC CCATCAAGTG GGCCCCGCCC GAAGTGCTCA ACTACACGCG CTTCTCCTCT 
AAGAGCGATG TGTGGGCATA CGGTGTGCTG ATGTGGGAGA TCTTCACTTG CGGCAAGATG 
CCATACGGTC GCCTAAAGAA TACCGAGGTT GTGGAGCGTG TGCAGCGCGG AATTATCCTA 
GAAAAACCAA AGTCGTGTGC CAAGGAGATT TATGATGTCA TGAAGTTGTG CTGGTCACAT 
GGACCCGAGG AGCGTCCCGC TTTCCGTGTG CTCATGGATC AGCTGGCTCT TGTGGCCCAA 
ACGCTAACCG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260244.1
CDS	1399..2793
Translation	MPGGIPTPGTPNSKAKDNSHFVKLVVALYPFKAIEGGDLSLEKNAEYEVIDDSQEHWWKVKDALGNVGYIPSNYVKPKALLGLERYEWYVGDMSRQRAESLLKQGDKEGCFVVRKSSTKGLYTLSLHTKVPQSHVKHYHIKQNARCEYYLSEKHCCETIPDLINYHRHNSGGLACRLKSSPCDRPVPPTAGLSHDKWEIHPMELMLMEELGSGQFGVVRRGKWRGSIDTAVKMMKEGTMSEDDFIEEAKVMTKLQHPNLVQLYGVCSKHRPIYIVTEYMKHGSLLNYLRRHEKTLIGNMGLLLDMCIQVSKGMTYLERHNYIHRDLAARNCLVGSENVVKVADFGLARYVLDDQYTSSGGTKFPIKWAPPEVLNYTRFSSKSDVWAYGVLMWEIFTCGKMPYGRLKNTEVVERVQRGIILEKPKSCAKEIYDVMKLCWSHGPEERPAFRVLMDQLALVAQTLTD

Target ORF information:

RefSeq Version	NM_001273315.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273315.1

ORF Insert Sequence:

ATGCCTGGCG GTATACCCAC TCCCGGAACA CCAAATTCCA AAGCCAAGGA CAACTCCCAC 
TTTGTCAAGC TGGTGGTGGC TCTCTATCCA TTCAAGGCCA TCGAAGGCGG CGATTTATCG 
CTGGAAAAGA ATGCCGAATA CGAAGTGATA GATGACTCAC AAGAGCACTG GTGGAAGGTC 
AAAGATGCCT TGGGGAATGT CGGCTATATA CCCAGCAACT ATGTCAAGCC GAAGGCTCTT 
CTGGGCCTGG AGCGCTATGA ATGGTATGTG GGCGACATGT CACGACAGAG AGCCGAATCG 
CTGCTGAAGC AAGGTGATAA GGAGGGCTGC TTTGTGGTCA GGAAGTCATC GACCAAGGGT 
CTCTACACAC TATCGCTGCA TACCAAAGTT CCACAGTCGC ATGTGAAGCA CTACCACATC 
AAGCAAAATG CTCGCTGCGA GTATTATTTG AGTGAGAAGC ACTGCTGTGA AACAATTCCG 
GATCTGATTA ACTACCATCG CCACAACTCT GGGGGTTTGG CTTGCCGACT CAAATCCTCG 
CCCTGCGATC GCCCAGTTCC ACCGACGGCG GGATTGTCCC ACGACAAATG GGAGATCCAT 
CCGATGGAAC TGATGCTGAT GGAGGAGCTT GGATCGGGAC AGTTTGGTGT TGTGCGGCGC 
GGCAAGTGGC GTGGGTCTAT CGACACAGCG GTCAAGATGA TGAAGGAAGG AACCATGTCC 
GAGGACGATT TCATTGAGGA GGCCAAGGTG ATGACCAAGC TGCAGCATCC AAATCTTGTG 
CAGCTATATG GCGTCTGCTC CAAGCACCGG CCCATCTACA TTGTGACCGA GTACATGAAG 
CACGGATCCT TGTTGAATTA CTTGCGACGG CATGAGAAGA CCCTGATTGG TAATATGGGT 
CTACTCCTTG ACATGTGCAT ACAGGTTAGC AAGGGAATGA CCTACCTGGA GCGCCATAAC 
TACATTCACC GGGATCTGGC TGCCCGCAAC TGTCTCGTGG GTTCCGAGAA TGTCGTTAAA 
GTGGCCGACT TTGGATTGGC CCGATACGTT CTCGACGATC AGTATACCAG CTCTGGCGGA 
ACCAAGTTCC CCATCAAGTG GGCCCCGCCC GAAGTGCTCA ACTACACGCG CTTCTCCTCT 
AAGAGCGATG TGTGGGCATA CGGTGTGCTG ATGTGGGAGA TCTTCACTTG CGGCAAGATG 
CCATACGGTC GCCTAAAGAA TACCGAGGTT GTGGAGCGTG TGCAGCGCGG AATTATCCTA 
GAAAAACCAA AGTCGTGTGC CAAGGAGATT TATGATGTCA TGAAGTTGTG CTGGTCACAT 
GGACCCGAGG AGCGTCCCGC TTTCCGTGTG CTCATGGATC AGCTGGCTCT TGTGGCCCAA 
ACGCTAACCG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Btk29A ( NM_001273314.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260243.1 Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa14118
Clone ID Related Accession (Same CDS sequence)	NM_001273314.1
Accession Version	NM_001273314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Btk family kinase at 29A, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGCTAT TATCGGCACT CAAGCTGGGC AACGGCAGTT CTCCAGCCCA GAATTCAACG 
CGCAGCATCA GTCCCAACAG CTCCACGACC AACAGTCAGT TTAGCTTGCA GCACAACAGC 
TCGGGGAGTC TCGGCGGAGG AGTGGGCGGC GGATTGGGAG GCGGAGGCAG CCTTGGCCTA 
GGCGGCGGAG GAGGAGGTGG CGGCAGTTGC ACGCCCACAT CGCTACAGCC TCAGTCCTCG 
CTGACAACTT TCAAGCAGTC CCCAACTCTA TTGAACGGCA ACGGAACTCT ATTGGATGCC 
AATATGCCTG GCGGTATACC CACTCCCGGA ACACCAAATT CCAAAGCCAA GGACAACTCC 
CACTTTGTCA AGCTGGTGGT GGCTCTCTAT CCATTCAAGG CCATCGAAGG CGGCGATTTA 
TCGCTGGAAA AGAATGCCGA ATACGAAGTG ATAGATGACT CACAAGAGCA CTGGTGGAAG 
GTCAAAGATG CCTTGGGGAA TGTCGGCTAT ATACCCAGCA ACTATGTCAA GCCGAAGGCT 
CTTCTGGGCC TGGAGCGCTA TGAATGGTAT GTGGGCGACA TGTCACGACA GAGAGCCGAA 
TCGCTGCTGA AGCAAGGTGA TAAGGAGGGC TGCTTTGTGG TCAGGAAGTC ATCGACCAAG 
GGTCTCTACA CACTATCGCT GCATACCAAA GTTCCACAGT CGCATGTGAA GCACTACCAC 
ATCAAGCAAA ATGCTCGCTG CGAGTATTAT TTGAGTGAGA AGCACTGCTG TGAAACAATT 
CCGGATCTGA TTAACTACCA TCGCCACAAC TCTGGGGGTT TGGCTTGCCG ACTCAAATCC 
TCGCCCTGCG ATCGCCCAGT TCCACCGACG GCGGGATTGT CCCACGACAA ATGGGAGATC 
CATCCGATGG AACTGATGCT GATGGAGGAG CTTGGATCGG GACAGTTTGG TGTTGTGCGG 
CGCGGCAAGT GGCGTGGGTC TATCGACACA GCGGTCAAGA TGATGAAGGA AGGAACCATG 
TCCGAGGACG ATTTCATTGA GGAGGCCAAG GTGATGACCA AGCTGCAGCA TCCAAATCTT 
GTGCAGCTAT ATGGCGTCTG CTCCAAGCAC CGGCCCATCT ACATTGTGAC CGAGTACATG 
AAGCACGGAT CCTTGTTGAA TTACTTGCGA CGGCATGAGA AGACCCTGAT TGGTAATATG 
GGTCTACTCC TTGACATGTG CATACAGGTT AGCAAGGGAA TGACCTACCT GGAGCGCCAT 
AACTACATTC ACCGGGATCT GGCTGCCCGC AACTGTCTCG TGGGTTCCGA GAATGTCGTT 
AAAGTGGCCG ACTTTGGATT GGCCCGATAC GTTCTCGACG ATCAGTATAC CAGCTCTGGC 
GGAACCAAGT TCCCCATCAA GTGGGCCCCG CCCGAAGTGC TCAACTACAC GCGCTTCTCC 
TCTAAGAGCG ATGTGTGGGC ATACGGTGTG CTGATGTGGG AGATCTTCAC TTGCGGCAAG 
ATGCCATACG GTCGCCTAAA GAATACCGAG GTTGTGGAGC GTGTGCAGCG CGGAATTATC 
CTAGAAAAAC CAAAGTCGTG TGCCAAGGAG ATTTATGATG TCATGAAGTT GTGCTGGTCA 
CATGGACCCG AGGAGCGTCC CGCTTTCCGT GTGCTCATGG ATCAGCTGGC TCTTGTGGCC 
CAAACGCTAA CCGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260243.1
CDS	572..2269
Translation	MMLLSALKLGNGSSPAQNSTRSISPNSSTTNSQFSLQHNSSGSLGGGVGGGLGGGGSLGLGGGGGGGGSCTPTSLQPQSSLTTFKQSPTLLNGNGTLLDANMPGGIPTPGTPNSKAKDNSHFVKLVVALYPFKAIEGGDLSLEKNAEYEVIDDSQEHWWKVKDALGNVGYIPSNYVKPKALLGLERYEWYVGDMSRQRAESLLKQGDKEGCFVVRKSSTKGLYTLSLHTKVPQSHVKHYHIKQNARCEYYLSEKHCCETIPDLINYHRHNSGGLACRLKSSPCDRPVPPTAGLSHDKWEIHPMELMLMEELGSGQFGVVRRGKWRGSIDTAVKMMKEGTMSEDDFIEEAKVMTKLQHPNLVQLYGVCSKHRPIYIVTEYMKHGSLLNYLRRHEKTLIGNMGLLLDMCIQVSKGMTYLERHNYIHRDLAARNCLVGSENVVKVADFGLARYVLDDQYTSSGGTKFPIKWAPPEVLNYTRFSSKSDVWAYGVLMWEIFTCGKMPYGRLKNTEVVERVQRGIILEKPKSCAKEIYDVMKLCWSHGPEERPAFRVLMDQLALVAQTLTD

Target ORF information:

RefSeq Version	NM_001273314.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273314.1

ORF Insert Sequence:

ATGATGCTAT TATCGGCACT CAAGCTGGGC AACGGCAGTT CTCCAGCCCA GAATTCAACG 
CGCAGCATCA GTCCCAACAG CTCCACGACC AACAGTCAGT TTAGCTTGCA GCACAACAGC 
TCGGGGAGTC TCGGCGGAGG AGTGGGCGGC GGATTGGGAG GCGGAGGCAG CCTTGGCCTA 
GGCGGCGGAG GAGGAGGTGG CGGCAGTTGC ACGCCCACAT CGCTACAGCC TCAGTCCTCG 
CTGACAACTT TCAAGCAGTC CCCAACTCTA TTGAACGGCA ACGGAACTCT ATTGGATGCC 
AATATGCCTG GCGGTATACC CACTCCCGGA ACACCAAATT CCAAAGCCAA GGACAACTCC 
CACTTTGTCA AGCTGGTGGT GGCTCTCTAT CCATTCAAGG CCATCGAAGG CGGCGATTTA 
TCGCTGGAAA AGAATGCCGA ATACGAAGTG ATAGATGACT CACAAGAGCA CTGGTGGAAG 
GTCAAAGATG CCTTGGGGAA TGTCGGCTAT ATACCCAGCA ACTATGTCAA GCCGAAGGCT 
CTTCTGGGCC TGGAGCGCTA TGAATGGTAT GTGGGCGACA TGTCACGACA GAGAGCCGAA 
TCGCTGCTGA AGCAAGGTGA TAAGGAGGGC TGCTTTGTGG TCAGGAAGTC ATCGACCAAG 
GGTCTCTACA CACTATCGCT GCATACCAAA GTTCCACAGT CGCATGTGAA GCACTACCAC 
ATCAAGCAAA ATGCTCGCTG CGAGTATTAT TTGAGTGAGA AGCACTGCTG TGAAACAATT 
CCGGATCTGA TTAACTACCA TCGCCACAAC TCTGGGGGTT TGGCTTGCCG ACTCAAATCC 
TCGCCCTGCG ATCGCCCAGT TCCACCGACG GCGGGATTGT CCCACGACAA ATGGGAGATC 
CATCCGATGG AACTGATGCT GATGGAGGAG CTTGGATCGG GACAGTTTGG TGTTGTGCGG 
CGCGGCAAGT GGCGTGGGTC TATCGACACA GCGGTCAAGA TGATGAAGGA AGGAACCATG 
TCCGAGGACG ATTTCATTGA GGAGGCCAAG GTGATGACCA AGCTGCAGCA TCCAAATCTT 
GTGCAGCTAT ATGGCGTCTG CTCCAAGCAC CGGCCCATCT ACATTGTGAC CGAGTACATG 
AAGCACGGAT CCTTGTTGAA TTACTTGCGA CGGCATGAGA AGACCCTGAT TGGTAATATG 
GGTCTACTCC TTGACATGTG CATACAGGTT AGCAAGGGAA TGACCTACCT GGAGCGCCAT 
AACTACATTC ACCGGGATCT GGCTGCCCGC AACTGTCTCG TGGGTTCCGA GAATGTCGTT 
AAAGTGGCCG ACTTTGGATT GGCCCGATAC GTTCTCGACG ATCAGTATAC CAGCTCTGGC 
GGAACCAAGT TCCCCATCAA GTGGGCCCCG CCCGAAGTGC TCAACTACAC GCGCTTCTCC 
TCTAAGAGCG ATGTGTGGGC ATACGGTGTG CTGATGTGGG AGATCTTCAC TTGCGGCAAG 
ATGCCATACG GTCGCCTAAA GAATACCGAG GTTGTGGAGC GTGTGCAGCG CGGAATTATC 
CTAGAAAAAC CAAAGTCGTG TGCCAAGGAG ATTTATGATG TCATGAAGTT GTGCTGGTCA 
CATGGACCCG AGGAGCGTCC CGCTTTCCGT GTGCTCATGG ATCAGCTGGC TCTTGTGGCC 
CAAACGCTAA CCGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Btk29A ( NM_001103650.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097120.1 Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa16469
Clone ID Related Accession (Same CDS sequence)	NM_001103650.2 , NM_057398.4 , NM_164805.2
Accession Version	NM_001103650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Btk family kinase at 29A, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001103650.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTCCCT GCGTGAGTTT GGCCGAAACG AGCGTCATTG GCAACATGAA GGAGCGGGTC 
AAGGAAATGA AGGTGTTCGG CTGCCGACTC AACTTCTGGA ACCACATTGG TCACAGCCTG 
ACCAGTTCCA AAACCAAAGA AGGCAACGGC AGTTCTCCAG CCCAGAATTC AACGCGCAGC 
ATCAGTCCCA ACAGCTCCAC GACCAACAGT CAGTTTAGCT TGCAGCACAA CAGCTCGGGG 
AGTCTCGGCG GAGGAGTGGG CGGCGGATTG GGAGGCGGAG GCAGCCTTGG CCTAGGCGGC 
GGAGGAGGAG GTGGCGGCAG TTGCACGCCC ACATCGCTAC AGCCTCAGTC CTCGCTGACA 
ACTTTCAAGC AGTCCCCAAC TCTATTGAAC GGCAACGGAA CTCTATTGGA TGCCAATATG 
CCTGGCGGTA TACCCACTCC CGGAACACCA AATTCCAAAG CCAAGGACAA CTCCCACTTT 
GTCAAGCTGG TGGTGGCTCT CTATCCATTC AAGGCCATCG AAGGCGGCGA TTTATCGCTG 
GAAAAGAATG CCGAATACGA AGTGATAGAT GACTCACAAG AGCACTGGTG GAAGGTCAAA 
GATGCCTTGG GGAATGTCGG CTATATACCC AGCAACTATG TCAAGCCGAA GGCTCTTCTG 
GGCCTGGAGC GCTATGAATG GTATGTGGGC GACATGTCAC GACAGAGAGC CGAATCGCTG 
CTGAAGCAAG GTGATAAGGA GGGCTGCTTT GTGGTCAGGA AGTCATCGAC CAAGGGTCTC 
TACACACTAT CGCTGCATAC CAAAGTTCCA CAGTCGCATG TGAAGCACTA CCACATCAAG 
CAAAATGCTC GCTGCGAGTA TTATTTGAGT GAGAAGCACT GCTGTGAAAC AATTCCGGAT 
CTGATTAACT ACCATCGCCA CAACTCTGGG GGTTTGGCTT GCCGACTCAA ATCCTCGCCC 
TGCGATCGCC CAGTTCCACC GACGGCGGGA TTGTCCCACG ACAAATGGGA GATCCATCCG 
ATGGAACTGA TGCTGATGGA GGAGCTTGGA TCGGGACAGT TTGGTGTTGT GCGGCGCGGC 
AAGTGGCGTG GGTCTATCGA CACAGCGGTC AAGATGATGA AGGAAGGAAC CATGTCCGAG 
GACGATTTCA TTGAGGAGGC CAAGGTGATG ACCAAGCTGC AGCATCCAAA TCTTGTGCAG 
CTATATGGCG TCTGCTCCAA GCACCGGCCC ATCTACATTG TGACCGAGTA CATGAAGCAC 
GGATCCTTGT TGAATTACTT GCGACGGCAT GAGAAGACCC TGATTGGTAA TATGGGTCTA 
CTCCTTGACA TGTGCATACA GGTTAGCAAG GGAATGACCT ACCTGGAGCG CCATAACTAC 
ATTCACCGGG ATCTGGCTGC CCGCAACTGT CTCGTGGGTT CCGAGAATGT CGTTAAAGTG 
GCCGACTTTG GATTGGCCCG ATACGTTCTC GACGATCAGT ATACCAGCTC TGGCGGAACC 
AAGTTCCCCA TCAAGTGGGC CCCGCCCGAA GTGCTCAACT ACACGCGCTT CTCCTCTAAG 
AGCGATGTGT GGGCATACGG TGTGCTGATG TGGGAGATCT TCACTTGCGG CAAGATGCCA 
TACGGTCGCC TAAAGAATAC CGAGGTTGTG GAGCGTGTGC AGCGCGGAAT TATCCTAGAA 
AAACCAAAGT CGTGTGCCAA GGAGATTTAT GATGTCATGA AGTTGTGCTG GTCACATGGA 
CCCGAGGAGC GTCCCGCTTT CCGTGTGCTC ATGGATCAGC TGGCTCTTGT GGCCCAAACG 
CTAACCGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097120.1
CDS	2148..3959
Translation	MIPCVSLAETSVIGNMKERVKEMKVFGCRLNFWNHIGHSLTSSKTKEGNGSSPAQNSTRSISPNSSTTNSQFSLQHNSSGSLGGGVGGGLGGGGSLGLGGGGGGGGSCTPTSLQPQSSLTTFKQSPTLLNGNGTLLDANMPGGIPTPGTPNSKAKDNSHFVKLVVALYPFKAIEGGDLSLEKNAEYEVIDDSQEHWWKVKDALGNVGYIPSNYVKPKALLGLERYEWYVGDMSRQRAESLLKQGDKEGCFVVRKSSTKGLYTLSLHTKVPQSHVKHYHIKQNARCEYYLSEKHCCETIPDLINYHRHNSGGLACRLKSSPCDRPVPPTAGLSHDKWEIHPMELMLMEELGSGQFGVVRRGKWRGSIDTAVKMMKEGTMSEDDFIEEAKVMTKLQHPNLVQLYGVCSKHRPIYIVTEYMKHGSLLNYLRRHEKTLIGNMGLLLDMCIQVSKGMTYLERHNYIHRDLAARNCLVGSENVVKVADFGLARYVLDDQYTSSGGTKFPIKWAPPEVLNYTRFSSKSDVWAYGVLMWEIFTCGKMPYGRLKNTEVVERVQRGIILEKPKSCAKEIYDVMKLCWSHGPEERPAFRVLMDQLALVAQTLTD

Target ORF information:

RefSeq Version	NM_001103650.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103650.2

ORF Insert Sequence:

ATGATTCCCT GCGTGAGTTT GGCCGAAACG AGCGTCATTG GCAACATGAA GGAGCGGGTC 
AAGGAAATGA AGGTGTTCGG CTGCCGACTC AACTTCTGGA ACCACATTGG TCACAGCCTG 
ACCAGTTCCA AAACCAAAGA AGGCAACGGC AGTTCTCCAG CCCAGAATTC AACGCGCAGC 
ATCAGTCCCA ACAGCTCCAC GACCAACAGT CAGTTTAGCT TGCAGCACAA CAGCTCGGGG 
AGTCTCGGCG GAGGAGTGGG CGGCGGATTG GGAGGCGGAG GCAGCCTTGG CCTAGGCGGC 
GGAGGAGGAG GTGGCGGCAG TTGCACGCCC ACATCGCTAC AGCCTCAGTC CTCGCTGACA 
ACTTTCAAGC AGTCCCCAAC TCTATTGAAC GGCAACGGAA CTCTATTGGA TGCCAATATG 
CCTGGCGGTA TACCCACTCC CGGAACACCA AATTCCAAAG CCAAGGACAA CTCCCACTTT 
GTCAAGCTGG TGGTGGCTCT CTATCCATTC AAGGCCATCG AAGGCGGCGA TTTATCGCTG 
GAAAAGAATG CCGAATACGA AGTGATAGAT GACTCACAAG AGCACTGGTG GAAGGTCAAA 
GATGCCTTGG GGAATGTCGG CTATATACCC AGCAACTATG TCAAGCCGAA GGCTCTTCTG 
GGCCTGGAGC GCTATGAATG GTATGTGGGC GACATGTCAC GACAGAGAGC CGAATCGCTG 
CTGAAGCAAG GTGATAAGGA GGGCTGCTTT GTGGTCAGGA AGTCATCGAC CAAGGGTCTC 
TACACACTAT CGCTGCATAC CAAAGTTCCA CAGTCGCATG TGAAGCACTA CCACATCAAG 
CAAAATGCTC GCTGCGAGTA TTATTTGAGT GAGAAGCACT GCTGTGAAAC AATTCCGGAT 
CTGATTAACT ACCATCGCCA CAACTCTGGG GGTTTGGCTT GCCGACTCAA ATCCTCGCCC 
TGCGATCGCC CAGTTCCACC GACGGCGGGA TTGTCCCACG ACAAATGGGA GATCCATCCG 
ATGGAACTGA TGCTGATGGA GGAGCTTGGA TCGGGACAGT TTGGTGTTGT GCGGCGCGGC 
AAGTGGCGTG GGTCTATCGA CACAGCGGTC AAGATGATGA AGGAAGGAAC CATGTCCGAG 
GACGATTTCA TTGAGGAGGC CAAGGTGATG ACCAAGCTGC AGCATCCAAA TCTTGTGCAG 
CTATATGGCG TCTGCTCCAA GCACCGGCCC ATCTACATTG TGACCGAGTA CATGAAGCAC 
GGATCCTTGT TGAATTACTT GCGACGGCAT GAGAAGACCC TGATTGGTAA TATGGGTCTA 
CTCCTTGACA TGTGCATACA GGTTAGCAAG GGAATGACCT ACCTGGAGCG CCATAACTAC 
ATTCACCGGG ATCTGGCTGC CCGCAACTGT CTCGTGGGTT CCGAGAATGT CGTTAAAGTG 
GCCGACTTTG GATTGGCCCG ATACGTTCTC GACGATCAGT ATACCAGCTC TGGCGGAACC 
AAGTTCCCCA TCAAGTGGGC CCCGCCCGAA GTGCTCAACT ACACGCGCTT CTCCTCTAAG 
AGCGATGTGT GGGCATACGG TGTGCTGATG TGGGAGATCT TCACTTGCGG CAAGATGCCA 
TACGGTCGCC TAAAGAATAC CGAGGTTGTG GAGCGTGTGC AGCGCGGAAT TATCCTAGAA 
AAACCAAAGT CGTGTGCCAA GGAGATTTAT GATGTCATGA AGTTGTGCTG GTCACATGGA 
CCCGAGGAGC GTCCCGCTTT CCGTGTGCTC ATGGATCAGC TGGCTCTTGT GGCCCAAACG 
CTAACCGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Btk29A ( NM_057398.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476746.1 Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16469
Clone ID Related Accession (Same CDS sequence)	NM_001103650.2 , NM_057398.4 , NM_164805.2
Accession Version	NM_057398.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Btk family kinase at 29A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057398.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTCCCT GCGTGAGTTT GGCCGAAACG AGCGTCATTG GCAACATGAA GGAGCGGGTC 
AAGGAAATGA AGGTGTTCGG CTGCCGACTC AACTTCTGGA ACCACATTGG TCACAGCCTG 
ACCAGTTCCA AAACCAAAGA AGGCAACGGC AGTTCTCCAG CCCAGAATTC AACGCGCAGC 
ATCAGTCCCA ACAGCTCCAC GACCAACAGT CAGTTTAGCT TGCAGCACAA CAGCTCGGGG 
AGTCTCGGCG GAGGAGTGGG CGGCGGATTG GGAGGCGGAG GCAGCCTTGG CCTAGGCGGC 
GGAGGAGGAG GTGGCGGCAG TTGCACGCCC ACATCGCTAC AGCCTCAGTC CTCGCTGACA 
ACTTTCAAGC AGTCCCCAAC TCTATTGAAC GGCAACGGAA CTCTATTGGA TGCCAATATG 
CCTGGCGGTA TACCCACTCC CGGAACACCA AATTCCAAAG CCAAGGACAA CTCCCACTTT 
GTCAAGCTGG TGGTGGCTCT CTATCCATTC AAGGCCATCG AAGGCGGCGA TTTATCGCTG 
GAAAAGAATG CCGAATACGA AGTGATAGAT GACTCACAAG AGCACTGGTG GAAGGTCAAA 
GATGCCTTGG GGAATGTCGG CTATATACCC AGCAACTATG TCAAGCCGAA GGCTCTTCTG 
GGCCTGGAGC GCTATGAATG GTATGTGGGC GACATGTCAC GACAGAGAGC CGAATCGCTG 
CTGAAGCAAG GTGATAAGGA GGGCTGCTTT GTGGTCAGGA AGTCATCGAC CAAGGGTCTC 
TACACACTAT CGCTGCATAC CAAAGTTCCA CAGTCGCATG TGAAGCACTA CCACATCAAG 
CAAAATGCTC GCTGCGAGTA TTATTTGAGT GAGAAGCACT GCTGTGAAAC AATTCCGGAT 
CTGATTAACT ACCATCGCCA CAACTCTGGG GGTTTGGCTT GCCGACTCAA ATCCTCGCCC 
TGCGATCGCC CAGTTCCACC GACGGCGGGA TTGTCCCACG ACAAATGGGA GATCCATCCG 
ATGGAACTGA TGCTGATGGA GGAGCTTGGA TCGGGACAGT TTGGTGTTGT GCGGCGCGGC 
AAGTGGCGTG GGTCTATCGA CACAGCGGTC AAGATGATGA AGGAAGGAAC CATGTCCGAG 
GACGATTTCA TTGAGGAGGC CAAGGTGATG ACCAAGCTGC AGCATCCAAA TCTTGTGCAG 
CTATATGGCG TCTGCTCCAA GCACCGGCCC ATCTACATTG TGACCGAGTA CATGAAGCAC 
GGATCCTTGT TGAATTACTT GCGACGGCAT GAGAAGACCC TGATTGGTAA TATGGGTCTA 
CTCCTTGACA TGTGCATACA GGTTAGCAAG GGAATGACCT ACCTGGAGCG CCATAACTAC 
ATTCACCGGG ATCTGGCTGC CCGCAACTGT CTCGTGGGTT CCGAGAATGT CGTTAAAGTG 
GCCGACTTTG GATTGGCCCG ATACGTTCTC GACGATCAGT ATACCAGCTC TGGCGGAACC 
AAGTTCCCCA TCAAGTGGGC CCCGCCCGAA GTGCTCAACT ACACGCGCTT CTCCTCTAAG 
AGCGATGTGT GGGCATACGG TGTGCTGATG TGGGAGATCT TCACTTGCGG CAAGATGCCA 
TACGGTCGCC TAAAGAATAC CGAGGTTGTG GAGCGTGTGC AGCGCGGAAT TATCCTAGAA 
AAACCAAAGT CGTGTGCCAA GGAGATTTAT GATGTCATGA AGTTGTGCTG GTCACATGGA 
CCCGAGGAGC GTCCCGCTTT CCGTGTGCTC ATGGATCAGC TGGCTCTTGT GGCCCAAACG 
CTAACCGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476746.1
CDS	432..2243
Translation	MIPCVSLAETSVIGNMKERVKEMKVFGCRLNFWNHIGHSLTSSKTKEGNGSSPAQNSTRSISPNSSTTNSQFSLQHNSSGSLGGGVGGGLGGGGSLGLGGGGGGGGSCTPTSLQPQSSLTTFKQSPTLLNGNGTLLDANMPGGIPTPGTPNSKAKDNSHFVKLVVALYPFKAIEGGDLSLEKNAEYEVIDDSQEHWWKVKDALGNVGYIPSNYVKPKALLGLERYEWYVGDMSRQRAESLLKQGDKEGCFVVRKSSTKGLYTLSLHTKVPQSHVKHYHIKQNARCEYYLSEKHCCETIPDLINYHRHNSGGLACRLKSSPCDRPVPPTAGLSHDKWEIHPMELMLMEELGSGQFGVVRRGKWRGSIDTAVKMMKEGTMSEDDFIEEAKVMTKLQHPNLVQLYGVCSKHRPIYIVTEYMKHGSLLNYLRRHEKTLIGNMGLLLDMCIQVSKGMTYLERHNYIHRDLAARNCLVGSENVVKVADFGLARYVLDDQYTSSGGTKFPIKWAPPEVLNYTRFSSKSDVWAYGVLMWEIFTCGKMPYGRLKNTEVVERVQRGIILEKPKSCAKEIYDVMKLCWSHGPEERPAFRVLMDQLALVAQTLTD

Target ORF information:

RefSeq Version	NM_057398.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057398.4

ORF Insert Sequence:

ATGATTCCCT GCGTGAGTTT GGCCGAAACG AGCGTCATTG GCAACATGAA GGAGCGGGTC 
AAGGAAATGA AGGTGTTCGG CTGCCGACTC AACTTCTGGA ACCACATTGG TCACAGCCTG 
ACCAGTTCCA AAACCAAAGA AGGCAACGGC AGTTCTCCAG CCCAGAATTC AACGCGCAGC 
ATCAGTCCCA ACAGCTCCAC GACCAACAGT CAGTTTAGCT TGCAGCACAA CAGCTCGGGG 
AGTCTCGGCG GAGGAGTGGG CGGCGGATTG GGAGGCGGAG GCAGCCTTGG CCTAGGCGGC 
GGAGGAGGAG GTGGCGGCAG TTGCACGCCC ACATCGCTAC AGCCTCAGTC CTCGCTGACA 
ACTTTCAAGC AGTCCCCAAC TCTATTGAAC GGCAACGGAA CTCTATTGGA TGCCAATATG 
CCTGGCGGTA TACCCACTCC CGGAACACCA AATTCCAAAG CCAAGGACAA CTCCCACTTT 
GTCAAGCTGG TGGTGGCTCT CTATCCATTC AAGGCCATCG AAGGCGGCGA TTTATCGCTG 
GAAAAGAATG CCGAATACGA AGTGATAGAT GACTCACAAG AGCACTGGTG GAAGGTCAAA 
GATGCCTTGG GGAATGTCGG CTATATACCC AGCAACTATG TCAAGCCGAA GGCTCTTCTG 
GGCCTGGAGC GCTATGAATG GTATGTGGGC GACATGTCAC GACAGAGAGC CGAATCGCTG 
CTGAAGCAAG GTGATAAGGA GGGCTGCTTT GTGGTCAGGA AGTCATCGAC CAAGGGTCTC 
TACACACTAT CGCTGCATAC CAAAGTTCCA CAGTCGCATG TGAAGCACTA CCACATCAAG 
CAAAATGCTC GCTGCGAGTA TTATTTGAGT GAGAAGCACT GCTGTGAAAC AATTCCGGAT 
CTGATTAACT ACCATCGCCA CAACTCTGGG GGTTTGGCTT GCCGACTCAA ATCCTCGCCC 
TGCGATCGCC CAGTTCCACC GACGGCGGGA TTGTCCCACG ACAAATGGGA GATCCATCCG 
ATGGAACTGA TGCTGATGGA GGAGCTTGGA TCGGGACAGT TTGGTGTTGT GCGGCGCGGC 
AAGTGGCGTG GGTCTATCGA CACAGCGGTC AAGATGATGA AGGAAGGAAC CATGTCCGAG 
GACGATTTCA TTGAGGAGGC CAAGGTGATG ACCAAGCTGC AGCATCCAAA TCTTGTGCAG 
CTATATGGCG TCTGCTCCAA GCACCGGCCC ATCTACATTG TGACCGAGTA CATGAAGCAC 
GGATCCTTGT TGAATTACTT GCGACGGCAT GAGAAGACCC TGATTGGTAA TATGGGTCTA 
CTCCTTGACA TGTGCATACA GGTTAGCAAG GGAATGACCT ACCTGGAGCG CCATAACTAC 
ATTCACCGGG ATCTGGCTGC CCGCAACTGT CTCGTGGGTT CCGAGAATGT CGTTAAAGTG 
GCCGACTTTG GATTGGCCCG ATACGTTCTC GACGATCAGT ATACCAGCTC TGGCGGAACC 
AAGTTCCCCA TCAAGTGGGC CCCGCCCGAA GTGCTCAACT ACACGCGCTT CTCCTCTAAG 
AGCGATGTGT GGGCATACGG TGTGCTGATG TGGGAGATCT TCACTTGCGG CAAGATGCCA 
TACGGTCGCC TAAAGAATAC CGAGGTTGTG GAGCGTGTGC AGCGCGGAAT TATCCTAGAA 
AAACCAAAGT CGTGTGCCAA GGAGATTTAT GATGTCATGA AGTTGTGCTG GTCACATGGA 
CCCGAGGAGC GTCCCGCTTT CCGTGTGCTC ATGGATCAGC TGGCTCTTGT GGCCCAAACG 
CTAACCGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Btk29A ( NM_164805.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723370.1 Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa16469
Clone ID Related Accession (Same CDS sequence)	NM_001103650.2 , NM_057398.4 , NM_164805.2
Accession Version	NM_164805.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Btk family kinase at 29A, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164805.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTCCCT GCGTGAGTTT GGCCGAAACG AGCGTCATTG GCAACATGAA GGAGCGGGTC 
AAGGAAATGA AGGTGTTCGG CTGCCGACTC AACTTCTGGA ACCACATTGG TCACAGCCTG 
ACCAGTTCCA AAACCAAAGA AGGCAACGGC AGTTCTCCAG CCCAGAATTC AACGCGCAGC 
ATCAGTCCCA ACAGCTCCAC GACCAACAGT CAGTTTAGCT TGCAGCACAA CAGCTCGGGG 
AGTCTCGGCG GAGGAGTGGG CGGCGGATTG GGAGGCGGAG GCAGCCTTGG CCTAGGCGGC 
GGAGGAGGAG GTGGCGGCAG TTGCACGCCC ACATCGCTAC AGCCTCAGTC CTCGCTGACA 
ACTTTCAAGC AGTCCCCAAC TCTATTGAAC GGCAACGGAA CTCTATTGGA TGCCAATATG 
CCTGGCGGTA TACCCACTCC CGGAACACCA AATTCCAAAG CCAAGGACAA CTCCCACTTT 
GTCAAGCTGG TGGTGGCTCT CTATCCATTC AAGGCCATCG AAGGCGGCGA TTTATCGCTG 
GAAAAGAATG CCGAATACGA AGTGATAGAT GACTCACAAG AGCACTGGTG GAAGGTCAAA 
GATGCCTTGG GGAATGTCGG CTATATACCC AGCAACTATG TCAAGCCGAA GGCTCTTCTG 
GGCCTGGAGC GCTATGAATG GTATGTGGGC GACATGTCAC GACAGAGAGC CGAATCGCTG 
CTGAAGCAAG GTGATAAGGA GGGCTGCTTT GTGGTCAGGA AGTCATCGAC CAAGGGTCTC 
TACACACTAT CGCTGCATAC CAAAGTTCCA CAGTCGCATG TGAAGCACTA CCACATCAAG 
CAAAATGCTC GCTGCGAGTA TTATTTGAGT GAGAAGCACT GCTGTGAAAC AATTCCGGAT 
CTGATTAACT ACCATCGCCA CAACTCTGGG GGTTTGGCTT GCCGACTCAA ATCCTCGCCC 
TGCGATCGCC CAGTTCCACC GACGGCGGGA TTGTCCCACG ACAAATGGGA GATCCATCCG 
ATGGAACTGA TGCTGATGGA GGAGCTTGGA TCGGGACAGT TTGGTGTTGT GCGGCGCGGC 
AAGTGGCGTG GGTCTATCGA CACAGCGGTC AAGATGATGA AGGAAGGAAC CATGTCCGAG 
GACGATTTCA TTGAGGAGGC CAAGGTGATG ACCAAGCTGC AGCATCCAAA TCTTGTGCAG 
CTATATGGCG TCTGCTCCAA GCACCGGCCC ATCTACATTG TGACCGAGTA CATGAAGCAC 
GGATCCTTGT TGAATTACTT GCGACGGCAT GAGAAGACCC TGATTGGTAA TATGGGTCTA 
CTCCTTGACA TGTGCATACA GGTTAGCAAG GGAATGACCT ACCTGGAGCG CCATAACTAC 
ATTCACCGGG ATCTGGCTGC CCGCAACTGT CTCGTGGGTT CCGAGAATGT CGTTAAAGTG 
GCCGACTTTG GATTGGCCCG ATACGTTCTC GACGATCAGT ATACCAGCTC TGGCGGAACC 
AAGTTCCCCA TCAAGTGGGC CCCGCCCGAA GTGCTCAACT ACACGCGCTT CTCCTCTAAG 
AGCGATGTGT GGGCATACGG TGTGCTGATG TGGGAGATCT TCACTTGCGG CAAGATGCCA 
TACGGTCGCC TAAAGAATAC CGAGGTTGTG GAGCGTGTGC AGCGCGGAAT TATCCTAGAA 
AAACCAAAGT CGTGTGCCAA GGAGATTTAT GATGTCATGA AGTTGTGCTG GTCACATGGA 
CCCGAGGAGC GTCCCGCTTT CCGTGTGCTC ATGGATCAGC TGGCTCTTGT GGCCCAAACG 
CTAACCGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723370.1
CDS	435..2246
Translation	MIPCVSLAETSVIGNMKERVKEMKVFGCRLNFWNHIGHSLTSSKTKEGNGSSPAQNSTRSISPNSSTTNSQFSLQHNSSGSLGGGVGGGLGGGGSLGLGGGGGGGGSCTPTSLQPQSSLTTFKQSPTLLNGNGTLLDANMPGGIPTPGTPNSKAKDNSHFVKLVVALYPFKAIEGGDLSLEKNAEYEVIDDSQEHWWKVKDALGNVGYIPSNYVKPKALLGLERYEWYVGDMSRQRAESLLKQGDKEGCFVVRKSSTKGLYTLSLHTKVPQSHVKHYHIKQNARCEYYLSEKHCCETIPDLINYHRHNSGGLACRLKSSPCDRPVPPTAGLSHDKWEIHPMELMLMEELGSGQFGVVRRGKWRGSIDTAVKMMKEGTMSEDDFIEEAKVMTKLQHPNLVQLYGVCSKHRPIYIVTEYMKHGSLLNYLRRHEKTLIGNMGLLLDMCIQVSKGMTYLERHNYIHRDLAARNCLVGSENVVKVADFGLARYVLDDQYTSSGGTKFPIKWAPPEVLNYTRFSSKSDVWAYGVLMWEIFTCGKMPYGRLKNTEVVERVQRGIILEKPKSCAKEIYDVMKLCWSHGPEERPAFRVLMDQLALVAQTLTD

Target ORF information:

RefSeq Version	NM_164805.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164805.2

ORF Insert Sequence:

ATGATTCCCT GCGTGAGTTT GGCCGAAACG AGCGTCATTG GCAACATGAA GGAGCGGGTC 
AAGGAAATGA AGGTGTTCGG CTGCCGACTC AACTTCTGGA ACCACATTGG TCACAGCCTG 
ACCAGTTCCA AAACCAAAGA AGGCAACGGC AGTTCTCCAG CCCAGAATTC AACGCGCAGC 
ATCAGTCCCA ACAGCTCCAC GACCAACAGT CAGTTTAGCT TGCAGCACAA CAGCTCGGGG 
AGTCTCGGCG GAGGAGTGGG CGGCGGATTG GGAGGCGGAG GCAGCCTTGG CCTAGGCGGC 
GGAGGAGGAG GTGGCGGCAG TTGCACGCCC ACATCGCTAC AGCCTCAGTC CTCGCTGACA 
ACTTTCAAGC AGTCCCCAAC TCTATTGAAC GGCAACGGAA CTCTATTGGA TGCCAATATG 
CCTGGCGGTA TACCCACTCC CGGAACACCA AATTCCAAAG CCAAGGACAA CTCCCACTTT 
GTCAAGCTGG TGGTGGCTCT CTATCCATTC AAGGCCATCG AAGGCGGCGA TTTATCGCTG 
GAAAAGAATG CCGAATACGA AGTGATAGAT GACTCACAAG AGCACTGGTG GAAGGTCAAA 
GATGCCTTGG GGAATGTCGG CTATATACCC AGCAACTATG TCAAGCCGAA GGCTCTTCTG 
GGCCTGGAGC GCTATGAATG GTATGTGGGC GACATGTCAC GACAGAGAGC CGAATCGCTG 
CTGAAGCAAG GTGATAAGGA GGGCTGCTTT GTGGTCAGGA AGTCATCGAC CAAGGGTCTC 
TACACACTAT CGCTGCATAC CAAAGTTCCA CAGTCGCATG TGAAGCACTA CCACATCAAG 
CAAAATGCTC GCTGCGAGTA TTATTTGAGT GAGAAGCACT GCTGTGAAAC AATTCCGGAT 
CTGATTAACT ACCATCGCCA CAACTCTGGG GGTTTGGCTT GCCGACTCAA ATCCTCGCCC 
TGCGATCGCC CAGTTCCACC GACGGCGGGA TTGTCCCACG ACAAATGGGA GATCCATCCG 
ATGGAACTGA TGCTGATGGA GGAGCTTGGA TCGGGACAGT TTGGTGTTGT GCGGCGCGGC 
AAGTGGCGTG GGTCTATCGA CACAGCGGTC AAGATGATGA AGGAAGGAAC CATGTCCGAG 
GACGATTTCA TTGAGGAGGC CAAGGTGATG ACCAAGCTGC AGCATCCAAA TCTTGTGCAG 
CTATATGGCG TCTGCTCCAA GCACCGGCCC ATCTACATTG TGACCGAGTA CATGAAGCAC 
GGATCCTTGT TGAATTACTT GCGACGGCAT GAGAAGACCC TGATTGGTAA TATGGGTCTA 
CTCCTTGACA TGTGCATACA GGTTAGCAAG GGAATGACCT ACCTGGAGCG CCATAACTAC 
ATTCACCGGG ATCTGGCTGC CCGCAACTGT CTCGTGGGTT CCGAGAATGT CGTTAAAGTG 
GCCGACTTTG GATTGGCCCG ATACGTTCTC GACGATCAGT ATACCAGCTC TGGCGGAACC 
AAGTTCCCCA TCAAGTGGGC CCCGCCCGAA GTGCTCAACT ACACGCGCTT CTCCTCTAAG 
AGCGATGTGT GGGCATACGG TGTGCTGATG TGGGAGATCT TCACTTGCGG CAAGATGCCA 
TACGGTCGCC TAAAGAATAC CGAGGTTGTG GAGCGTGTGC AGCGCGGAAT TATCCTAGAA 
AAACCAAAGT CGTGTGCCAA GGAGATTTAT GATGTCATGA AGTTGTGCTG GTCACATGGA 
CCCGAGGAGC GTCCCGCTTT CCGTGTGCTC ATGGATCAGC TGGCTCTTGT GGCCCAAACG 
CTAACCGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Btk29A ( NM_164804.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723369.1 Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant D, mRNA.

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CloneID	OFa16445
Clone ID Related Accession (Same CDS sequence)	NM_164804.2 , NM_001103651.3 , NM_057397.6
Accession Version	NM_164804.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Btk family kinase at 29A, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164804.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGGGCA CTAAGCATCG AAACTCGCAT GTCAATGGCA GCATTAAGTC ATCATCATCG 
CTGCGATCGA GCAGCAAATC CTTTCAAGCA AAAATGGATC TGATGTCCGA AAGGCTGTAC 
GATGTCGTGA AAAGTGGATC GATGGTGAAA AGGGCTCAAA ACAAGAAGCG TTTCACCCCC 
GTGAATTACA AACATCGATG GTTTGAGTTG ACCAAGCGAA CTTTCAGCTA CTTCGATGTG 
GAAAATGTGG AGCGGAGACG CGAACGTGGT CGCATTCATT TGAAGGGCGT TCGCCTGGTG 
GAGGAGGCGA CGGTCAGTGG CGAGGGCGGC GATCCCTTTG CACCGGATGG TTATCCGTTC 
CAAGTGGGCT ACTGTGAGAT CTCTGCGTCG GCCAATTCGC ATCAGCTGGA GAACGGCAAC 
GGCGGTGGTA GTGGCGTTGG AATCGAGGGT CAGCAGAGTG GACGAGCGGT GCCACAGTAC 
ACACTCTACG TGATCGCCAA CAGCGAAAAG GAGCGCAGCG AGTGGATCCG CGCCATCCGC 
CAAGTTTGTG AGGATAGTAA TACTCCAAAA TCGTATCGCT ATCATCCGGG CTTGTGGTCT 
GGAAAGAAGT GGAGCTGCTG CAAGGGATTA TCTCGAACCA CATTTGGTTG CAGAGCAGCC 
GCCCATTGGC GTGAGGCCAA CAATAATCCA AGCAACGGCA GTTCTCCAGC CCAGAATTCA 
ACGCGCAGCA TCAGTCCCAA CAGCTCCACG ACCAACAGTC AGTTTAGCTT GCAGCACAAC 
AGCTCGGGGA GTCTCGGCGG AGGAGTGGGC GGCGGATTGG GAGGCGGAGG CAGCCTTGGC 
CTAGGCGGCG GAGGAGGAGG TGGCGGCAGT TGCACGCCCA CATCGCTACA GCCTCAGTCC 
TCGCTGACAA CTTTCAAGCA GTCCCCAACT CTATTGAACG GCAACGGAAC TCTATTGGAT 
GCCAATATGC CTGGCGGTAT ACCCACTCCC GGAACACCAA ATTCCAAAGC CAAGGACAAC 
TCCCACTTTG TCAAGCTGGT GGTGGCTCTC TATCCATTCA AGGCCATCGA AGGCGGCGAT 
TTATCGCTGG AAAAGAATGC CGAATACGAA GTGATAGATG ACTCACAAGA GCACTGGTGG 
AAGGTCAAAG ATGCCTTGGG GAATGTCGGC TATATACCCA GCAACTATGT CAAGCCGAAG 
GCTCTTCTGG GCCTGGAGCG CTATGAATGG TATGTGGGCG ACATGTCACG ACAGAGAGCC 
GAATCGCTGC TGAAGCAAGG TGATAAGGAG GGCTGCTTTG TGGTCAGGAA GTCATCGACC 
AAGGGTCTCT ACACACTATC GCTGCATACC AAAGTTCCAC AGTCGCATGT GAAGCACTAC 
CACATCAAGC AAAATGCTCG CTGCGAGTAT TATTTGAGTG AGAAGCACTG CTGTGAAACA 
ATTCCGGATC TGATTAACTA CCATCGCCAC AACTCTGGGG GTTTGGCTTG CCGACTCAAA 
TCCTCGCCCT GCGATCGCCC AGTTCCACCG ACGGCGGGAT TGTCCCACGA CAAATGGGAG 
ATCCATCCGA TGGAACTGAT GCTGATGGAG GAGCTTGGAT CGGGACAGTT TGGTGTTGTG 
CGGCGCGGCA AGTGGCGTGG GTCTATCGAC ACAGCGGTCA AGATGATGAA GGAAGGAACC 
ATGTCCGAGG ACGATTTCAT TGAGGAGGCC AAGGTGATGA CCAAGCTGCA GCATCCAAAT 
CTTGTGCAGC TATATGGCGT CTGCTCCAAG CACCGGCCCA TCTACATTGT GACCGAGTAC 
ATGAAGCACG GATCCTTGTT GAATTACTTG CGACGGCATG AGAAGACCCT GATTGGTAAT 
ATGGGTCTAC TCCTTGACAT GTGCATACAG GTTAGCAAGG GAATGACCTA CCTGGAGCGC 
CATAACTACA TTCACCGGGA TCTGGCTGCC CGCAACTGTC TCGTGGGTTC CGAGAATGTC 
GTTAAAGTGG CCGACTTTGG ATTGGCCCGA TACGTTCTCG ACGATCAGTA TACCAGCTCT 
GGCGGAACCA AGTTCCCCAT CAAGTGGGCC CCGCCCGAAG TGCTCAACTA CACGCGCTTC 
TCCTCTAAGA GCGATGTGTG GGCATACGGT GTGCTGATGT GGGAGATCTT CACTTGCGGC 
AAGATGCCAT ACGGTCGCCT AAAGAATACC GAGGTTGTGG AGCGTGTGCA GCGCGGAATT 
ATCCTAGAAA AACCAAAGTC GTGTGCCAAG GAGATTTATG ATGTCATGAA GTTGTGCTGG 
TCACATGGAC CCGAGGAGCG TCCCGCTTTC CGTGTGCTCA TGGATCAGCT GGCTCTTGTG 
GCCCAAACGC TAACCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723369.1
CDS	281..2641
Translation	MMGTKHRNSHVNGSIKSSSSLRSSSKSFQAKMDLMSERLYDVVKSGSMVKRAQNKKRFTPVNYKHRWFELTKRTFSYFDVENVERRRERGRIHLKGVRLVEEATVSGEGGDPFAPDGYPFQVGYCEISASANSHQLENGNGGGSGVGIEGQQSGRAVPQYTLYVIANSEKERSEWIRAIRQVCEDSNTPKSYRYHPGLWSGKKWSCCKGLSRTTFGCRAAAHWREANNNPSNGSSPAQNSTRSISPNSSTTNSQFSLQHNSSGSLGGGVGGGLGGGGSLGLGGGGGGGGSCTPTSLQPQSSLTTFKQSPTLLNGNGTLLDANMPGGIPTPGTPNSKAKDNSHFVKLVVALYPFKAIEGGDLSLEKNAEYEVIDDSQEHWWKVKDALGNVGYIPSNYVKPKALLGLERYEWYVGDMSRQRAESLLKQGDKEGCFVVRKSSTKGLYTLSLHTKVPQSHVKHYHIKQNARCEYYLSEKHCCETIPDLINYHRHNSGGLACRLKSSPCDRPVPPTAGLSHDKWEIHPMELMLMEELGSGQFGVVRRGKWRGSIDTAVKMMKEGTMSEDDFIEEAKVMTKLQHPNLVQLYGVCSKHRPIYIVTEYMKHGSLLNYLRRHEKTLIGNMGLLLDMCIQVSKGMTYLERHNYIHRDLAARNCLVGSENVVKVADFGLARYVLDDQYTSSGGTKFPIKWAPPEVLNYTRFSSKSDVWAYGVLMWEIFTCGKMPYGRLKNTEVVERVQRGIILEKPKSCAKEIYDVMKLCWSHGPEERPAFRVLMDQLALVAQTLTD

Target ORF information:

RefSeq Version	NM_164804.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164804.2

ORF Insert Sequence:

ATGATGGGCA CTAAGCATCG AAACTCGCAT GTCAATGGCA GCATTAAGTC ATCATCATCG 
CTGCGATCGA GCAGCAAATC CTTTCAAGCA AAAATGGATC TGATGTCCGA AAGGCTGTAC 
GATGTCGTGA AAAGTGGATC GATGGTGAAA AGGGCTCAAA ACAAGAAGCG TTTCACCCCC 
GTGAATTACA AACATCGATG GTTTGAGTTG ACCAAGCGAA CTTTCAGCTA CTTCGATGTG 
GAAAATGTGG AGCGGAGACG CGAACGTGGT CGCATTCATT TGAAGGGCGT TCGCCTGGTG 
GAGGAGGCGA CGGTCAGTGG CGAGGGCGGC GATCCCTTTG CACCGGATGG TTATCCGTTC 
CAAGTGGGCT ACTGTGAGAT CTCTGCGTCG GCCAATTCGC ATCAGCTGGA GAACGGCAAC 
GGCGGTGGTA GTGGCGTTGG AATCGAGGGT CAGCAGAGTG GACGAGCGGT GCCACAGTAC 
ACACTCTACG TGATCGCCAA CAGCGAAAAG GAGCGCAGCG AGTGGATCCG CGCCATCCGC 
CAAGTTTGTG AGGATAGTAA TACTCCAAAA TCGTATCGCT ATCATCCGGG CTTGTGGTCT 
GGAAAGAAGT GGAGCTGCTG CAAGGGATTA TCTCGAACCA CATTTGGTTG CAGAGCAGCC 
GCCCATTGGC GTGAGGCCAA CAATAATCCA AGCAACGGCA GTTCTCCAGC CCAGAATTCA 
ACGCGCAGCA TCAGTCCCAA CAGCTCCACG ACCAACAGTC AGTTTAGCTT GCAGCACAAC 
AGCTCGGGGA GTCTCGGCGG AGGAGTGGGC GGCGGATTGG GAGGCGGAGG CAGCCTTGGC 
CTAGGCGGCG GAGGAGGAGG TGGCGGCAGT TGCACGCCCA CATCGCTACA GCCTCAGTCC 
TCGCTGACAA CTTTCAAGCA GTCCCCAACT CTATTGAACG GCAACGGAAC TCTATTGGAT 
GCCAATATGC CTGGCGGTAT ACCCACTCCC GGAACACCAA ATTCCAAAGC CAAGGACAAC 
TCCCACTTTG TCAAGCTGGT GGTGGCTCTC TATCCATTCA AGGCCATCGA AGGCGGCGAT 
TTATCGCTGG AAAAGAATGC CGAATACGAA GTGATAGATG ACTCACAAGA GCACTGGTGG 
AAGGTCAAAG ATGCCTTGGG GAATGTCGGC TATATACCCA GCAACTATGT CAAGCCGAAG 
GCTCTTCTGG GCCTGGAGCG CTATGAATGG TATGTGGGCG ACATGTCACG ACAGAGAGCC 
GAATCGCTGC TGAAGCAAGG TGATAAGGAG GGCTGCTTTG TGGTCAGGAA GTCATCGACC 
AAGGGTCTCT ACACACTATC GCTGCATACC AAAGTTCCAC AGTCGCATGT GAAGCACTAC 
CACATCAAGC AAAATGCTCG CTGCGAGTAT TATTTGAGTG AGAAGCACTG CTGTGAAACA 
ATTCCGGATC TGATTAACTA CCATCGCCAC AACTCTGGGG GTTTGGCTTG CCGACTCAAA 
TCCTCGCCCT GCGATCGCCC AGTTCCACCG ACGGCGGGAT TGTCCCACGA CAAATGGGAG 
ATCCATCCGA TGGAACTGAT GCTGATGGAG GAGCTTGGAT CGGGACAGTT TGGTGTTGTG 
CGGCGCGGCA AGTGGCGTGG GTCTATCGAC ACAGCGGTCA AGATGATGAA GGAAGGAACC 
ATGTCCGAGG ACGATTTCAT TGAGGAGGCC AAGGTGATGA CCAAGCTGCA GCATCCAAAT 
CTTGTGCAGC TATATGGCGT CTGCTCCAAG CACCGGCCCA TCTACATTGT GACCGAGTAC 
ATGAAGCACG GATCCTTGTT GAATTACTTG CGACGGCATG AGAAGACCCT GATTGGTAAT 
ATGGGTCTAC TCCTTGACAT GTGCATACAG GTTAGCAAGG GAATGACCTA CCTGGAGCGC 
CATAACTACA TTCACCGGGA TCTGGCTGCC CGCAACTGTC TCGTGGGTTC CGAGAATGTC 
GTTAAAGTGG CCGACTTTGG ATTGGCCCGA TACGTTCTCG ACGATCAGTA TACCAGCTCT 
GGCGGAACCA AGTTCCCCAT CAAGTGGGCC CCGCCCGAAG TGCTCAACTA CACGCGCTTC 
TCCTCTAAGA GCGATGTGTG GGCATACGGT GTGCTGATGT GGGAGATCTT CACTTGCGGC 
AAGATGCCAT ACGGTCGCCT AAAGAATACC GAGGTTGTGG AGCGTGTGCA GCGCGGAATT 
ATCCTAGAAA AACCAAAGTC GTGTGCCAAG GAGATTTATG ATGTCATGAA GTTGTGCTGG 
TCACATGGAC CCGAGGAGCG TCCCGCTTTC CGTGTGCTCA TGGATCAGCT GGCTCTTGTG 
GCCCAAACGC TAACCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Btk29A ( NM_001103651.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097121.1 Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa16445
Clone ID Related Accession (Same CDS sequence)	NM_164804.2 , NM_001103651.3 , NM_057397.6
Accession Version	NM_001103651.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Btk family kinase at 29A, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001103651.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGGGCA CTAAGCATCG AAACTCGCAT GTCAATGGCA GCATTAAGTC ATCATCATCG 
CTGCGATCGA GCAGCAAATC CTTTCAAGCA AAAATGGATC TGATGTCCGA AAGGCTGTAC 
GATGTCGTGA AAAGTGGATC GATGGTGAAA AGGGCTCAAA ACAAGAAGCG TTTCACCCCC 
GTGAATTACA AACATCGATG GTTTGAGTTG ACCAAGCGAA CTTTCAGCTA CTTCGATGTG 
GAAAATGTGG AGCGGAGACG CGAACGTGGT CGCATTCATT TGAAGGGCGT TCGCCTGGTG 
GAGGAGGCGA CGGTCAGTGG CGAGGGCGGC GATCCCTTTG CACCGGATGG TTATCCGTTC 
CAAGTGGGCT ACTGTGAGAT CTCTGCGTCG GCCAATTCGC ATCAGCTGGA GAACGGCAAC 
GGCGGTGGTA GTGGCGTTGG AATCGAGGGT CAGCAGAGTG GACGAGCGGT GCCACAGTAC 
ACACTCTACG TGATCGCCAA CAGCGAAAAG GAGCGCAGCG AGTGGATCCG CGCCATCCGC 
CAAGTTTGTG AGGATAGTAA TACTCCAAAA TCGTATCGCT ATCATCCGGG CTTGTGGTCT 
GGAAAGAAGT GGAGCTGCTG CAAGGGATTA TCTCGAACCA CATTTGGTTG CAGAGCAGCC 
GCCCATTGGC GTGAGGCCAA CAATAATCCA AGCAACGGCA GTTCTCCAGC CCAGAATTCA 
ACGCGCAGCA TCAGTCCCAA CAGCTCCACG ACCAACAGTC AGTTTAGCTT GCAGCACAAC 
AGCTCGGGGA GTCTCGGCGG AGGAGTGGGC GGCGGATTGG GAGGCGGAGG CAGCCTTGGC 
CTAGGCGGCG GAGGAGGAGG TGGCGGCAGT TGCACGCCCA CATCGCTACA GCCTCAGTCC 
TCGCTGACAA CTTTCAAGCA GTCCCCAACT CTATTGAACG GCAACGGAAC TCTATTGGAT 
GCCAATATGC CTGGCGGTAT ACCCACTCCC GGAACACCAA ATTCCAAAGC CAAGGACAAC 
TCCCACTTTG TCAAGCTGGT GGTGGCTCTC TATCCATTCA AGGCCATCGA AGGCGGCGAT 
TTATCGCTGG AAAAGAATGC CGAATACGAA GTGATAGATG ACTCACAAGA GCACTGGTGG 
AAGGTCAAAG ATGCCTTGGG GAATGTCGGC TATATACCCA GCAACTATGT CAAGCCGAAG 
GCTCTTCTGG GCCTGGAGCG CTATGAATGG TATGTGGGCG ACATGTCACG ACAGAGAGCC 
GAATCGCTGC TGAAGCAAGG TGATAAGGAG GGCTGCTTTG TGGTCAGGAA GTCATCGACC 
AAGGGTCTCT ACACACTATC GCTGCATACC AAAGTTCCAC AGTCGCATGT GAAGCACTAC 
CACATCAAGC AAAATGCTCG CTGCGAGTAT TATTTGAGTG AGAAGCACTG CTGTGAAACA 
ATTCCGGATC TGATTAACTA CCATCGCCAC AACTCTGGGG GTTTGGCTTG CCGACTCAAA 
TCCTCGCCCT GCGATCGCCC AGTTCCACCG ACGGCGGGAT TGTCCCACGA CAAATGGGAG 
ATCCATCCGA TGGAACTGAT GCTGATGGAG GAGCTTGGAT CGGGACAGTT TGGTGTTGTG 
CGGCGCGGCA AGTGGCGTGG GTCTATCGAC ACAGCGGTCA AGATGATGAA GGAAGGAACC 
ATGTCCGAGG ACGATTTCAT TGAGGAGGCC AAGGTGATGA CCAAGCTGCA GCATCCAAAT 
CTTGTGCAGC TATATGGCGT CTGCTCCAAG CACCGGCCCA TCTACATTGT GACCGAGTAC 
ATGAAGCACG GATCCTTGTT GAATTACTTG CGACGGCATG AGAAGACCCT GATTGGTAAT 
ATGGGTCTAC TCCTTGACAT GTGCATACAG GTTAGCAAGG GAATGACCTA CCTGGAGCGC 
CATAACTACA TTCACCGGGA TCTGGCTGCC CGCAACTGTC TCGTGGGTTC CGAGAATGTC 
GTTAAAGTGG CCGACTTTGG ATTGGCCCGA TACGTTCTCG ACGATCAGTA TACCAGCTCT 
GGCGGAACCA AGTTCCCCAT CAAGTGGGCC CCGCCCGAAG TGCTCAACTA CACGCGCTTC 
TCCTCTAAGA GCGATGTGTG GGCATACGGT GTGCTGATGT GGGAGATCTT CACTTGCGGC 
AAGATGCCAT ACGGTCGCCT AAAGAATACC GAGGTTGTGG AGCGTGTGCA GCGCGGAATT 
ATCCTAGAAA AACCAAAGTC GTGTGCCAAG GAGATTTATG ATGTCATGAA GTTGTGCTGG 
TCACATGGAC CCGAGGAGCG TCCCGCTTTC CGTGTGCTCA TGGATCAGCT GGCTCTTGTG 
GCCCAAACGC TAACCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097121.1
CDS	260..2620
Translation	MMGTKHRNSHVNGSIKSSSSLRSSSKSFQAKMDLMSERLYDVVKSGSMVKRAQNKKRFTPVNYKHRWFELTKRTFSYFDVENVERRRERGRIHLKGVRLVEEATVSGEGGDPFAPDGYPFQVGYCEISASANSHQLENGNGGGSGVGIEGQQSGRAVPQYTLYVIANSEKERSEWIRAIRQVCEDSNTPKSYRYHPGLWSGKKWSCCKGLSRTTFGCRAAAHWREANNNPSNGSSPAQNSTRSISPNSSTTNSQFSLQHNSSGSLGGGVGGGLGGGGSLGLGGGGGGGGSCTPTSLQPQSSLTTFKQSPTLLNGNGTLLDANMPGGIPTPGTPNSKAKDNSHFVKLVVALYPFKAIEGGDLSLEKNAEYEVIDDSQEHWWKVKDALGNVGYIPSNYVKPKALLGLERYEWYVGDMSRQRAESLLKQGDKEGCFVVRKSSTKGLYTLSLHTKVPQSHVKHYHIKQNARCEYYLSEKHCCETIPDLINYHRHNSGGLACRLKSSPCDRPVPPTAGLSHDKWEIHPMELMLMEELGSGQFGVVRRGKWRGSIDTAVKMMKEGTMSEDDFIEEAKVMTKLQHPNLVQLYGVCSKHRPIYIVTEYMKHGSLLNYLRRHEKTLIGNMGLLLDMCIQVSKGMTYLERHNYIHRDLAARNCLVGSENVVKVADFGLARYVLDDQYTSSGGTKFPIKWAPPEVLNYTRFSSKSDVWAYGVLMWEIFTCGKMPYGRLKNTEVVERVQRGIILEKPKSCAKEIYDVMKLCWSHGPEERPAFRVLMDQLALVAQTLTD

Target ORF information:

RefSeq Version	NM_001103651.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103651.3

ORF Insert Sequence:

ATGATGGGCA CTAAGCATCG AAACTCGCAT GTCAATGGCA GCATTAAGTC ATCATCATCG 
CTGCGATCGA GCAGCAAATC CTTTCAAGCA AAAATGGATC TGATGTCCGA AAGGCTGTAC 
GATGTCGTGA AAAGTGGATC GATGGTGAAA AGGGCTCAAA ACAAGAAGCG TTTCACCCCC 
GTGAATTACA AACATCGATG GTTTGAGTTG ACCAAGCGAA CTTTCAGCTA CTTCGATGTG 
GAAAATGTGG AGCGGAGACG CGAACGTGGT CGCATTCATT TGAAGGGCGT TCGCCTGGTG 
GAGGAGGCGA CGGTCAGTGG CGAGGGCGGC GATCCCTTTG CACCGGATGG TTATCCGTTC 
CAAGTGGGCT ACTGTGAGAT CTCTGCGTCG GCCAATTCGC ATCAGCTGGA GAACGGCAAC 
GGCGGTGGTA GTGGCGTTGG AATCGAGGGT CAGCAGAGTG GACGAGCGGT GCCACAGTAC 
ACACTCTACG TGATCGCCAA CAGCGAAAAG GAGCGCAGCG AGTGGATCCG CGCCATCCGC 
CAAGTTTGTG AGGATAGTAA TACTCCAAAA TCGTATCGCT ATCATCCGGG CTTGTGGTCT 
GGAAAGAAGT GGAGCTGCTG CAAGGGATTA TCTCGAACCA CATTTGGTTG CAGAGCAGCC 
GCCCATTGGC GTGAGGCCAA CAATAATCCA AGCAACGGCA GTTCTCCAGC CCAGAATTCA 
ACGCGCAGCA TCAGTCCCAA CAGCTCCACG ACCAACAGTC AGTTTAGCTT GCAGCACAAC 
AGCTCGGGGA GTCTCGGCGG AGGAGTGGGC GGCGGATTGG GAGGCGGAGG CAGCCTTGGC 
CTAGGCGGCG GAGGAGGAGG TGGCGGCAGT TGCACGCCCA CATCGCTACA GCCTCAGTCC 
TCGCTGACAA CTTTCAAGCA GTCCCCAACT CTATTGAACG GCAACGGAAC TCTATTGGAT 
GCCAATATGC CTGGCGGTAT ACCCACTCCC GGAACACCAA ATTCCAAAGC CAAGGACAAC 
TCCCACTTTG TCAAGCTGGT GGTGGCTCTC TATCCATTCA AGGCCATCGA AGGCGGCGAT 
TTATCGCTGG AAAAGAATGC CGAATACGAA GTGATAGATG ACTCACAAGA GCACTGGTGG 
AAGGTCAAAG ATGCCTTGGG GAATGTCGGC TATATACCCA GCAACTATGT CAAGCCGAAG 
GCTCTTCTGG GCCTGGAGCG CTATGAATGG TATGTGGGCG ACATGTCACG ACAGAGAGCC 
GAATCGCTGC TGAAGCAAGG TGATAAGGAG GGCTGCTTTG TGGTCAGGAA GTCATCGACC 
AAGGGTCTCT ACACACTATC GCTGCATACC AAAGTTCCAC AGTCGCATGT GAAGCACTAC 
CACATCAAGC AAAATGCTCG CTGCGAGTAT TATTTGAGTG AGAAGCACTG CTGTGAAACA 
ATTCCGGATC TGATTAACTA CCATCGCCAC AACTCTGGGG GTTTGGCTTG CCGACTCAAA 
TCCTCGCCCT GCGATCGCCC AGTTCCACCG ACGGCGGGAT TGTCCCACGA CAAATGGGAG 
ATCCATCCGA TGGAACTGAT GCTGATGGAG GAGCTTGGAT CGGGACAGTT TGGTGTTGTG 
CGGCGCGGCA AGTGGCGTGG GTCTATCGAC ACAGCGGTCA AGATGATGAA GGAAGGAACC 
ATGTCCGAGG ACGATTTCAT TGAGGAGGCC AAGGTGATGA CCAAGCTGCA GCATCCAAAT 
CTTGTGCAGC TATATGGCGT CTGCTCCAAG CACCGGCCCA TCTACATTGT GACCGAGTAC 
ATGAAGCACG GATCCTTGTT GAATTACTTG CGACGGCATG AGAAGACCCT GATTGGTAAT 
ATGGGTCTAC TCCTTGACAT GTGCATACAG GTTAGCAAGG GAATGACCTA CCTGGAGCGC 
CATAACTACA TTCACCGGGA TCTGGCTGCC CGCAACTGTC TCGTGGGTTC CGAGAATGTC 
GTTAAAGTGG CCGACTTTGG ATTGGCCCGA TACGTTCTCG ACGATCAGTA TACCAGCTCT 
GGCGGAACCA AGTTCCCCAT CAAGTGGGCC CCGCCCGAAG TGCTCAACTA CACGCGCTTC 
TCCTCTAAGA GCGATGTGTG GGCATACGGT GTGCTGATGT GGGAGATCTT CACTTGCGGC 
AAGATGCCAT ACGGTCGCCT AAAGAATACC GAGGTTGTGG AGCGTGTGCA GCGCGGAATT 
ATCCTAGAAA AACCAAAGTC GTGTGCCAAG GAGATTTATG ATGTCATGAA GTTGTGCTGG 
TCACATGGAC CCGAGGAGCG TCCCGCTTTC CGTGTGCTCA TGGATCAGCT GGCTCTTGTG 
GCCCAAACGC TAACCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Btk29A ( NM_057397.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476745.1 Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16445
Clone ID Related Accession (Same CDS sequence)	NM_164804.2 , NM_001103651.3 , NM_057397.6
Accession Version	NM_057397.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Btk family kinase at 29A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057397.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGGGCA CTAAGCATCG AAACTCGCAT GTCAATGGCA GCATTAAGTC ATCATCATCG 
CTGCGATCGA GCAGCAAATC CTTTCAAGCA AAAATGGATC TGATGTCCGA AAGGCTGTAC 
GATGTCGTGA AAAGTGGATC GATGGTGAAA AGGGCTCAAA ACAAGAAGCG TTTCACCCCC 
GTGAATTACA AACATCGATG GTTTGAGTTG ACCAAGCGAA CTTTCAGCTA CTTCGATGTG 
GAAAATGTGG AGCGGAGACG CGAACGTGGT CGCATTCATT TGAAGGGCGT TCGCCTGGTG 
GAGGAGGCGA CGGTCAGTGG CGAGGGCGGC GATCCCTTTG CACCGGATGG TTATCCGTTC 
CAAGTGGGCT ACTGTGAGAT CTCTGCGTCG GCCAATTCGC ATCAGCTGGA GAACGGCAAC 
GGCGGTGGTA GTGGCGTTGG AATCGAGGGT CAGCAGAGTG GACGAGCGGT GCCACAGTAC 
ACACTCTACG TGATCGCCAA CAGCGAAAAG GAGCGCAGCG AGTGGATCCG CGCCATCCGC 
CAAGTTTGTG AGGATAGTAA TACTCCAAAA TCGTATCGCT ATCATCCGGG CTTGTGGTCT 
GGAAAGAAGT GGAGCTGCTG CAAGGGATTA TCTCGAACCA CATTTGGTTG CAGAGCAGCC 
GCCCATTGGC GTGAGGCCAA CAATAATCCA AGCAACGGCA GTTCTCCAGC CCAGAATTCA 
ACGCGCAGCA TCAGTCCCAA CAGCTCCACG ACCAACAGTC AGTTTAGCTT GCAGCACAAC 
AGCTCGGGGA GTCTCGGCGG AGGAGTGGGC GGCGGATTGG GAGGCGGAGG CAGCCTTGGC 
CTAGGCGGCG GAGGAGGAGG TGGCGGCAGT TGCACGCCCA CATCGCTACA GCCTCAGTCC 
TCGCTGACAA CTTTCAAGCA GTCCCCAACT CTATTGAACG GCAACGGAAC TCTATTGGAT 
GCCAATATGC CTGGCGGTAT ACCCACTCCC GGAACACCAA ATTCCAAAGC CAAGGACAAC 
TCCCACTTTG TCAAGCTGGT GGTGGCTCTC TATCCATTCA AGGCCATCGA AGGCGGCGAT 
TTATCGCTGG AAAAGAATGC CGAATACGAA GTGATAGATG ACTCACAAGA GCACTGGTGG 
AAGGTCAAAG ATGCCTTGGG GAATGTCGGC TATATACCCA GCAACTATGT CAAGCCGAAG 
GCTCTTCTGG GCCTGGAGCG CTATGAATGG TATGTGGGCG ACATGTCACG ACAGAGAGCC 
GAATCGCTGC TGAAGCAAGG TGATAAGGAG GGCTGCTTTG TGGTCAGGAA GTCATCGACC 
AAGGGTCTCT ACACACTATC GCTGCATACC AAAGTTCCAC AGTCGCATGT GAAGCACTAC 
CACATCAAGC AAAATGCTCG CTGCGAGTAT TATTTGAGTG AGAAGCACTG CTGTGAAACA 
ATTCCGGATC TGATTAACTA CCATCGCCAC AACTCTGGGG GTTTGGCTTG CCGACTCAAA 
TCCTCGCCCT GCGATCGCCC AGTTCCACCG ACGGCGGGAT TGTCCCACGA CAAATGGGAG 
ATCCATCCGA TGGAACTGAT GCTGATGGAG GAGCTTGGAT CGGGACAGTT TGGTGTTGTG 
CGGCGCGGCA AGTGGCGTGG GTCTATCGAC ACAGCGGTCA AGATGATGAA GGAAGGAACC 
ATGTCCGAGG ACGATTTCAT TGAGGAGGCC AAGGTGATGA CCAAGCTGCA GCATCCAAAT 
CTTGTGCAGC TATATGGCGT CTGCTCCAAG CACCGGCCCA TCTACATTGT GACCGAGTAC 
ATGAAGCACG GATCCTTGTT GAATTACTTG CGACGGCATG AGAAGACCCT GATTGGTAAT 
ATGGGTCTAC TCCTTGACAT GTGCATACAG GTTAGCAAGG GAATGACCTA CCTGGAGCGC 
CATAACTACA TTCACCGGGA TCTGGCTGCC CGCAACTGTC TCGTGGGTTC CGAGAATGTC 
GTTAAAGTGG CCGACTTTGG ATTGGCCCGA TACGTTCTCG ACGATCAGTA TACCAGCTCT 
GGCGGAACCA AGTTCCCCAT CAAGTGGGCC CCGCCCGAAG TGCTCAACTA CACGCGCTTC 
TCCTCTAAGA GCGATGTGTG GGCATACGGT GTGCTGATGT GGGAGATCTT CACTTGCGGC 
AAGATGCCAT ACGGTCGCCT AAAGAATACC GAGGTTGTGG AGCGTGTGCA GCGCGGAATT 
ATCCTAGAAA AACCAAAGTC GTGTGCCAAG GAGATTTATG ATGTCATGAA GTTGTGCTGG 
TCACATGGAC CCGAGGAGCG TCCCGCTTTC CGTGTGCTCA TGGATCAGCT GGCTCTTGTG 
GCCCAAACGC TAACCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476745.1
CDS	593..2953
Translation	MMGTKHRNSHVNGSIKSSSSLRSSSKSFQAKMDLMSERLYDVVKSGSMVKRAQNKKRFTPVNYKHRWFELTKRTFSYFDVENVERRRERGRIHLKGVRLVEEATVSGEGGDPFAPDGYPFQVGYCEISASANSHQLENGNGGGSGVGIEGQQSGRAVPQYTLYVIANSEKERSEWIRAIRQVCEDSNTPKSYRYHPGLWSGKKWSCCKGLSRTTFGCRAAAHWREANNNPSNGSSPAQNSTRSISPNSSTTNSQFSLQHNSSGSLGGGVGGGLGGGGSLGLGGGGGGGGSCTPTSLQPQSSLTTFKQSPTLLNGNGTLLDANMPGGIPTPGTPNSKAKDNSHFVKLVVALYPFKAIEGGDLSLEKNAEYEVIDDSQEHWWKVKDALGNVGYIPSNYVKPKALLGLERYEWYVGDMSRQRAESLLKQGDKEGCFVVRKSSTKGLYTLSLHTKVPQSHVKHYHIKQNARCEYYLSEKHCCETIPDLINYHRHNSGGLACRLKSSPCDRPVPPTAGLSHDKWEIHPMELMLMEELGSGQFGVVRRGKWRGSIDTAVKMMKEGTMSEDDFIEEAKVMTKLQHPNLVQLYGVCSKHRPIYIVTEYMKHGSLLNYLRRHEKTLIGNMGLLLDMCIQVSKGMTYLERHNYIHRDLAARNCLVGSENVVKVADFGLARYVLDDQYTSSGGTKFPIKWAPPEVLNYTRFSSKSDVWAYGVLMWEIFTCGKMPYGRLKNTEVVERVQRGIILEKPKSCAKEIYDVMKLCWSHGPEERPAFRVLMDQLALVAQTLTD

Target ORF information:

RefSeq Version	NM_057397.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Btk family kinase at 29A (Btk29A), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057397.6

ORF Insert Sequence:

ATGATGGGCA CTAAGCATCG AAACTCGCAT GTCAATGGCA GCATTAAGTC ATCATCATCG 
CTGCGATCGA GCAGCAAATC CTTTCAAGCA AAAATGGATC TGATGTCCGA AAGGCTGTAC 
GATGTCGTGA AAAGTGGATC GATGGTGAAA AGGGCTCAAA ACAAGAAGCG TTTCACCCCC 
GTGAATTACA AACATCGATG GTTTGAGTTG ACCAAGCGAA CTTTCAGCTA CTTCGATGTG 
GAAAATGTGG AGCGGAGACG CGAACGTGGT CGCATTCATT TGAAGGGCGT TCGCCTGGTG 
GAGGAGGCGA CGGTCAGTGG CGAGGGCGGC GATCCCTTTG CACCGGATGG TTATCCGTTC 
CAAGTGGGCT ACTGTGAGAT CTCTGCGTCG GCCAATTCGC ATCAGCTGGA GAACGGCAAC 
GGCGGTGGTA GTGGCGTTGG AATCGAGGGT CAGCAGAGTG GACGAGCGGT GCCACAGTAC 
ACACTCTACG TGATCGCCAA CAGCGAAAAG GAGCGCAGCG AGTGGATCCG CGCCATCCGC 
CAAGTTTGTG AGGATAGTAA TACTCCAAAA TCGTATCGCT ATCATCCGGG CTTGTGGTCT 
GGAAAGAAGT GGAGCTGCTG CAAGGGATTA TCTCGAACCA CATTTGGTTG CAGAGCAGCC 
GCCCATTGGC GTGAGGCCAA CAATAATCCA AGCAACGGCA GTTCTCCAGC CCAGAATTCA 
ACGCGCAGCA TCAGTCCCAA CAGCTCCACG ACCAACAGTC AGTTTAGCTT GCAGCACAAC 
AGCTCGGGGA GTCTCGGCGG AGGAGTGGGC GGCGGATTGG GAGGCGGAGG CAGCCTTGGC 
CTAGGCGGCG GAGGAGGAGG TGGCGGCAGT TGCACGCCCA CATCGCTACA GCCTCAGTCC 
TCGCTGACAA CTTTCAAGCA GTCCCCAACT CTATTGAACG GCAACGGAAC TCTATTGGAT 
GCCAATATGC CTGGCGGTAT ACCCACTCCC GGAACACCAA ATTCCAAAGC CAAGGACAAC 
TCCCACTTTG TCAAGCTGGT GGTGGCTCTC TATCCATTCA AGGCCATCGA AGGCGGCGAT 
TTATCGCTGG AAAAGAATGC CGAATACGAA GTGATAGATG ACTCACAAGA GCACTGGTGG 
AAGGTCAAAG ATGCCTTGGG GAATGTCGGC TATATACCCA GCAACTATGT CAAGCCGAAG 
GCTCTTCTGG GCCTGGAGCG CTATGAATGG TATGTGGGCG ACATGTCACG ACAGAGAGCC 
GAATCGCTGC TGAAGCAAGG TGATAAGGAG GGCTGCTTTG TGGTCAGGAA GTCATCGACC 
AAGGGTCTCT ACACACTATC GCTGCATACC AAAGTTCCAC AGTCGCATGT GAAGCACTAC 
CACATCAAGC AAAATGCTCG CTGCGAGTAT TATTTGAGTG AGAAGCACTG CTGTGAAACA 
ATTCCGGATC TGATTAACTA CCATCGCCAC AACTCTGGGG GTTTGGCTTG CCGACTCAAA 
TCCTCGCCCT GCGATCGCCC AGTTCCACCG ACGGCGGGAT TGTCCCACGA CAAATGGGAG 
ATCCATCCGA TGGAACTGAT GCTGATGGAG GAGCTTGGAT CGGGACAGTT TGGTGTTGTG 
CGGCGCGGCA AGTGGCGTGG GTCTATCGAC ACAGCGGTCA AGATGATGAA GGAAGGAACC 
ATGTCCGAGG ACGATTTCAT TGAGGAGGCC AAGGTGATGA CCAAGCTGCA GCATCCAAAT 
CTTGTGCAGC TATATGGCGT CTGCTCCAAG CACCGGCCCA TCTACATTGT GACCGAGTAC 
ATGAAGCACG GATCCTTGTT GAATTACTTG CGACGGCATG AGAAGACCCT GATTGGTAAT 
ATGGGTCTAC TCCTTGACAT GTGCATACAG GTTAGCAAGG GAATGACCTA CCTGGAGCGC 
CATAACTACA TTCACCGGGA TCTGGCTGCC CGCAACTGTC TCGTGGGTTC CGAGAATGTC 
GTTAAAGTGG CCGACTTTGG ATTGGCCCGA TACGTTCTCG ACGATCAGTA TACCAGCTCT 
GGCGGAACCA AGTTCCCCAT CAAGTGGGCC CCGCCCGAAG TGCTCAACTA CACGCGCTTC 
TCCTCTAAGA GCGATGTGTG GGCATACGGT GTGCTGATGT GGGAGATCTT CACTTGCGGC 
AAGATGCCAT ACGGTCGCCT AAAGAATACC GAGGTTGTGG AGCGTGTGCA GCGCGGAATT 
ATCCTAGAAA AACCAAAGTC GTGTGCCAAG GAGATTTATG ATGTCATGAA GTTGTGCTGG 
TCACATGGAC CCGAGGAGCG TCCCGCTTTC CGTGTGCTCA TGGATCAGCT GGCTCTTGTG 
GCCCAAACGC TAACCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Btk29A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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