CG8475 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG8475
Entrez Gene ID	34114
Full Name	CG8475 gene product from transcript CG8475-RC
Synonyms	Dmel\CG8475
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 28F1-28F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273301.1	NP_001260230.1	uncharacterized protein, isoform C
NM_135345.3	NP_609189.2	uncharacterized protein, isoform A
NM_164797.3	NP_723356.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	PHKB	NP_000284.1
Caenorhabditis elegans (roundworm)	Y67D8A.2	NP_741355.3
Xenopus tropicalis (tropical clawed frog)	phkb	XP_004914116.1
	PHKB	XP_001113858.1
Danio rerio (zebrafish)	phkb	XP_698197.5
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009278	XP_320073.4
Canis lupus familiaris (dog)	PHKB	XP_005617652.1
Mus musculus (house mouse)	Phkb	NP_955517.1
Pan troglodytes (chimpanzee)	PHKB	XP_001162360.1
Bos taurus (cattle)	PHKB	XP_002694819.1
Rattus norvegicus (Norway rat)	Phkb	NP_001014174.1
Gallus gallus (chicken)	PHKB	NP_001007832.1
Drosophila melanogaster (fruit fly)	CG8475	NP_001260230.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG8475 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG8475 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14093	NM_164797.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8475), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14092	NM_135345.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8475), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa15944	NM_001273301.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG8475), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG8475 ( NM_164797.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723356.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG8475), mRNA.

Price & Availability↑

CloneID	OFa14093
Clone ID Related Accession (Same CDS sequence)	NM_164797.3
Accession Version	NM_164797.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164797.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCGATG TGCCGAAATC TCTCGGTCTC TCGGTCACAA CTCCAGGCGG CAGTTCGGGT 
GCTCCGGACA GTGGTCGCCA CAACAGCTTG GAGGAGATCA ATCTGGACCA GTTCCTGAAA 
ACGTCCAACT ACGAGGACAC GGTGAAGCAG CTGGACATCT ACTATGGCAT TGTCAAGCGT 
CAACTGCTGC GATACCAGAG TCCCATCACC GGTTTGTTTC CGGTGATGAG CACCGACCAG 
GTGGTGGGAT CGGTGCGGGA CAGTGTGTAT TGCGCATCCG CCGTGTGGAG CTTGTACCAG 
GCGTACAGAC GGATCGACGA TGACCGCGGA AAGTCCTACG AACTGGGCCA GAGCACTGTG 
AAGTGCATGC GAGGCATTCT TGAGTGCTGG GTCAAGCAGG CATCGCGGGT GGAGCTCTTT 
AAGCAGCGCC AGTCCAATCA GCACGCACTG CATAGCAAAT TCCAGCTGCA CACCGGAGAG 
AAGATATATC CGGATGAGTT TTACAATCAC CTACAGATAG ATTGCGTTTC TCTCTATCTG 
TTGTTCCTCG TCCAGATGAT AACCTCTGGC CTGCAGATCA TTTACACCCA CGACGAGGTG 
GCCTTTGTCC AGAATCTAGT GTACTATGTG GAGCGCGCCT ATCGTACGCC CGACTTTGGC 
ATGTGGGAGC GGGGCTCTAA ATATAACAAT GGCACCCCGG AGATCCATGC CTCCTCCATT 
GGCATGGCTA AGTCCGCCTT GGAGGCCATT AACGGGTGCA ATCTGTTCGG CGAGAAAGGA 
GCTTCATGGA GCGTTGTCTA TGTGGACATT GATGCCCACA ACCGGAATCG CAGTATTTTC 
GAAACCATGC TGCCCAGGGA ATCAAGCTCA AAGGGAGTTG ATGCTTCACT GCTTCTCACC 
CTTTCTTTCC CAGCCTTTGC CTCGCATGAA GATCGCCTGG TGGAGCAGAC CAAACAAAAT 
GTTGTAAATC GTTTGCGCTG CAAAATGGGC TTTAAGCGCT TCACCCGTGA TGGATTCCTC 
AGCAAAAACG AGGACAAATC GCGACGCTAT TATCACTCTG GCGAGCTAAA GGAATTCGAA 
GGCCTGGAAT GTGAATGGCC GCTCTTTTTC ATAGCCATGA TTATCGATGG CGTGTTCAAG 
AATAACAATG AACAAATTGA AGAGTTCCAA AACGATTTGA GGCGCTGTTT GCGTACTGAT 
GTCAATGGAG ATCCCGTAGT GACCATGTAC TATGCGCCGG ATGGAGATGG CTCCTACATG 
CGTGCTCCAT CGCAATCGCT GTTCCTCTGG GGGCAGTCCT TCTTTATCAT AGCCCAGCTG 
CTGACCGCAG GACTCCTACA CATCAATGAA CTGGATCCAA TCCGCCGCTA CTTGCCAAGC 
TATAATCGGC CTAGAAGGGC GGGTCGTTAT TCGGCTTTCC AGGTGAGTTG GAATCGGGAT 
TCTTGGAGGG CACTAAGCGC TATGAAGTGT TTTCTTAAAT TGTTAACCAA TGGCAAAGCA 
TTCTGGATCA CAATAGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723356.1
CDS	104..1624
Translation	MRDVPKSLGLSVTTPGGSSGAPDSGRHNSLEEINLDQFLKTSNYEDTVKQLDIYYGIVKRQLLRYQSPITGLFPVMSTDQVVGSVRDSVYCASAVWSLYQAYRRIDDDRGKSYELGQSTVKCMRGILECWVKQASRVELFKQRQSNQHALHSKFQLHTGEKIYPDEFYNHLQIDCVSLYLLFLVQMITSGLQIIYTHDEVAFVQNLVYYVERAYRTPDFGMWERGSKYNNGTPEIHASSIGMAKSALEAINGCNLFGEKGASWSVVYVDIDAHNRNRSIFETMLPRESSSKGVDASLLLTLSFPAFASHEDRLVEQTKQNVVNRLRCKMGFKRFTRDGFLSKNEDKSRRYYHSGELKEFEGLECEWPLFFIAMIIDGVFKNNNEQIEEFQNDLRRCLRTDVNGDPVVTMYYAPDGDGSYMRAPSQSLFLWGQSFFIIAQLLTAGLLHINELDPIRRYLPSYNRPRRAGRYSAFQVSWNRDSWRALSAMKCFLKLLTNGKAFWITIG

Target ORF information:

RefSeq Version	NM_164797.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8475), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164797.3

ORF Insert Sequence:

ATGCGCGATG TGCCGAAATC TCTCGGTCTC TCGGTCACAA CTCCAGGCGG CAGTTCGGGT 
GCTCCGGACA GTGGTCGCCA CAACAGCTTG GAGGAGATCA ATCTGGACCA GTTCCTGAAA 
ACGTCCAACT ACGAGGACAC GGTGAAGCAG CTGGACATCT ACTATGGCAT TGTCAAGCGT 
CAACTGCTGC GATACCAGAG TCCCATCACC GGTTTGTTTC CGGTGATGAG CACCGACCAG 
GTGGTGGGAT CGGTGCGGGA CAGTGTGTAT TGCGCATCCG CCGTGTGGAG CTTGTACCAG 
GCGTACAGAC GGATCGACGA TGACCGCGGA AAGTCCTACG AACTGGGCCA GAGCACTGTG 
AAGTGCATGC GAGGCATTCT TGAGTGCTGG GTCAAGCAGG CATCGCGGGT GGAGCTCTTT 
AAGCAGCGCC AGTCCAATCA GCACGCACTG CATAGCAAAT TCCAGCTGCA CACCGGAGAG 
AAGATATATC CGGATGAGTT TTACAATCAC CTACAGATAG ATTGCGTTTC TCTCTATCTG 
TTGTTCCTCG TCCAGATGAT AACCTCTGGC CTGCAGATCA TTTACACCCA CGACGAGGTG 
GCCTTTGTCC AGAATCTAGT GTACTATGTG GAGCGCGCCT ATCGTACGCC CGACTTTGGC 
ATGTGGGAGC GGGGCTCTAA ATATAACAAT GGCACCCCGG AGATCCATGC CTCCTCCATT 
GGCATGGCTA AGTCCGCCTT GGAGGCCATT AACGGGTGCA ATCTGTTCGG CGAGAAAGGA 
GCTTCATGGA GCGTTGTCTA TGTGGACATT GATGCCCACA ACCGGAATCG CAGTATTTTC 
GAAACCATGC TGCCCAGGGA ATCAAGCTCA AAGGGAGTTG ATGCTTCACT GCTTCTCACC 
CTTTCTTTCC CAGCCTTTGC CTCGCATGAA GATCGCCTGG TGGAGCAGAC CAAACAAAAT 
GTTGTAAATC GTTTGCGCTG CAAAATGGGC TTTAAGCGCT TCACCCGTGA TGGATTCCTC 
AGCAAAAACG AGGACAAATC GCGACGCTAT TATCACTCTG GCGAGCTAAA GGAATTCGAA 
GGCCTGGAAT GTGAATGGCC GCTCTTTTTC ATAGCCATGA TTATCGATGG CGTGTTCAAG 
AATAACAATG AACAAATTGA AGAGTTCCAA AACGATTTGA GGCGCTGTTT GCGTACTGAT 
GTCAATGGAG ATCCCGTAGT GACCATGTAC TATGCGCCGG ATGGAGATGG CTCCTACATG 
CGTGCTCCAT CGCAATCGCT GTTCCTCTGG GGGCAGTCCT TCTTTATCAT AGCCCAGCTG 
CTGACCGCAG GACTCCTACA CATCAATGAA CTGGATCCAA TCCGCCGCTA CTTGCCAAGC 
TATAATCGGC CTAGAAGGGC GGGTCGTTAT TCGGCTTTCC AGGTGAGTTG GAATCGGGAT 
TCTTGGAGGG CACTAAGCGC TATGAAGTGT TTTCTTAAAT TGTTAACCAA TGGCAAAGCA 
TTCTGGATCA CAATAGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8475 ( NM_135345.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609189.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG8475), mRNA.

Price & Availability↑

CloneID	OFa14092
Clone ID Related Accession (Same CDS sequence)	NM_135345.3
Accession Version	NM_135345.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135345.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCGATG TGCCGAAATC TCTCGGTCTC TCGGTCACAA CTCCAGGCGG CAGTTCGGGT 
GCTCCGGACA GTGGTCGCCA CAACAGCTTG GAGGAGATCA ATCTGGACCA GTTCCTGAAA 
ACGTCCAACT ACGAGGACAC GGTGAAGCAG CTGGACATCT ACTATGGCAT TGTCAAGCGT 
CAACTGCTGC GATACCAGAG TCCCATCACC GGTTTGTTTC CGGTGATGAG CACCGACCAG 
GTGGTGGGAT CGGTGCGGGA CAGTGTGTAT TGCGCATCCG CCGTGTGGAG CTTGTACCAG 
GCGTACAGAC GGATCGACGA TGACCGCGGA AAGTCCTACG AACTGGGCCA GAGCACTGTG 
AAGTGCATGC GAGGCATTCT TGAGTGCTGG GTCAAGCAGG CATCGCGGGT GGAGCTCTTT 
AAGCAGCGCC AGTCCAATCA GCACGCACTG CATAGCAAAT TCCAGCTGCA CACCGGAGAG 
AAGATATATC CGGATGAGTT TTACAATCAC CTACAGATAG ATTGCGTTTC TCTCTATCTG 
TTGTTCCTCG TCCAGATGAT AACCTCTGGC CTGCAGATCA TTTACACCCA CGACGAGGTG 
GCCTTTGTCC AGAATCTAGT GTACTATGTG GAGCGCGCCT ATCGTACGCC CGACTTTGGC 
ATGTGGGAGC GGGGCTCTAA ATATAACAAT GGCACCCCGG AGATCCATGC CTCCTCCATT 
GGCATGGCTA AGTCCGCCTT GGAGGCCATT AACGGGTGCA ATCTGTTCGG CGAGAAAGGA 
GCTTCATGGA GCGTTGTCTA TGTGGACATT GATGCCCACA ACCGGAATCG CAGTATTTTC 
GAAACCATGC TGCCCAGGGA ATCAAGCTCA AAGGGAGTTG ATGCTTCACT GCTTCTCACC 
CTTTCTTTCC CAGCCTTTGC CTCGCATGAA GATCGCCTGG TGGAGCAGAC CAAACAAAAT 
GTTGTAAATC GTTTGCGCTG CAAAATGGGC TTTAAGCGCT TCACCCGTGA TGGATTCCTC 
AGCAAAAACG AGGACAAATC GCGACGCTAT TATCACTCTG GCGAGCTAAA GGAATTCGAA 
GGCCTGGAAT GTGAATGGCC GCTCTTTTTC ATAGCCATGA TTATCGATGG CGTGTTCAAG 
AATAACAATG AACAAATTGA AGAGTTCCAA AACGATTTGA GGCGCTGTTT GCGTACTGAT 
GTCAATGGAG ATCCCGTAGT GACCATGTAC TATGCGCCGG ATGGAGATGG CTCCTACATG 
CGTGCTCCAT CGCAATCGCT GTTCCTCTGG GGGCAGTCCT TCTTTATCAT AGCCCAGCTG 
CTGACCGCAG GACTCCTACA CATCAATGAA CTGGATCCAA TCCGCCGCTA CTTGCCAAGC 
TATAATCGGC CTAGAAGGGC GGGTCGTTAT TCGGCTTTCC AGGGCACAGC CACTGATCTT 
GTGGTGCAGA TTGTCCTGAT TGCCGAGTCC ATGCGACTGC AGGCTATGAT GGCCACCTAT 
GGCATTCAAA CACAAACCCC CCATGAGGTG GAACCTGTGC AAATCTGGAG CTCTACAGAG 
CTCATTAAAG TATATCAACA TCTCGGAGTT AACAACAAGG TTGGCCTGTC CGGTCGTCCA 
TGCAGACCGG TGGGTTCTTT GGGCACCAGC AAGGTGTATC GCATCTGCGG CATGACCGTC 
CTCTGCTATC CACTAATCTT TGAGGTCTCC GACTTTTATC TCTACCGGGA CATGGCTCTA 
CTTATCGATG ACATCAAGAC AGAGCTACAG TTTGTGGGCA AGTACTGGCG GCTATCGGGC 
AGACCTACAG TTTGTCTGCT TATTCGCGAG GAGCACATGC GTGATCCGCA GTTTAAGGAG 
ATGCTCGACC TGCTGGCCAT GCTCAAAAAG GGCTACTGCG ATGGCATGAA AGTTCGCATC 
GGTCGTCTAC AGAATCTGAT TAGCAGCTCG TGCATCGAGC ATCTTGACTT TATGAACCAA 
AGTGATCTGA CCGACAACGA GAATGCGTTC TCGCAGATAA ACCATGAGTA TATTGGCTAC 
CAATCGCTAA CAGATGTGCC CAAAGCTCTG ACATATGTGG AGGAAAAGAT TTCTGTTGCG 
CACTTCGATA CCAAACCCAC GCCGGATATC ATCAACGCAC TACGTAGCAC GGATTCCATT 
TACTGTTTGT GCCAATTGTG GGGTATTATT CTCAACCGCG AGGGTCCTCA TTTTGAGGTA 
AACGGTCTGA ACGTAAACAC GGCCTTGACG CAGCTATACC ATCGCGCTGG TTCCTTGCGC 
TACTGGCGTG CCGTGCGATA CTGTTCCTCT CTTCTTCACC ACATTGTGGA CTCCATTAGT 
CCATTTATCA CCACTGTGTT GGTGAATGGC AAGGAGCTCA CAGTGGGCAT CATTGGCCAG 
AAGGAGACGG TATTCGACAA GCCAATGACG CCGGCGGAGA TTCAAAATGT CATGTACACG 
AGTGTCCAAC CCTACGACGT CATCCAGGCA GTGTTGCAGC AGGAAGTGGT TCTGTATTGT 
GGCCGTTTAA TAGCCACCAA TCCATCAATG TTTCGTGGCA TATTAAAAAT TCGCATTGGT 
TGGGTACTGG AGGCCATGCG CATCTATTTA CAAATCTCAG GACAACAGAG CATTGATGTT 
GACAACCTTT CGCCCTTCCA AGTCCGCATA CTTCTTCAGA AAGTCTTAAC CGTCAGCGAA 
TGGGCTGTAG AAGAAAAGCT CACTACCCTA CAACGTCGTC AACTGGAGGG ATGCTTGTGC 
CGTGTGCCAA AACATTTTTA TAACAAAATC TGGGAAATCC TACAGCGAAC CCCTCAAGGC 
ATACTGACCC AGGGGCATCA TTTGCCAGCC ACGCCCACAC TAACCAACAT GAGCCGCGGC 
GAGTTGACCT TCAATCTGCT GGTCGAGGAA ACTCTGATTT GCATTGACCG TCCGGAAAGG 
CGTCAGATAA CCGTGGAGCT TTTGTGCATT GTGGCCACCA TTCTTAATCG TAACCCCGAG 
CTGCACTTTA AGCAAGCTCT AGACTTGGAC GGCATCTTGG CGGAAGCGTT CGCAATGTAC 
TGCAAGGACA ACAACATACA GCATCAACCA AAACCAGAGC ATGAGCAGAC CAAAAATGAG 
GATCTCAAGG CTTTCTATTC GTTGCCATAT TCCGAGACCA CGGGCTATTT GGCTCGCGCC 
GCGGTCAACA AGGTTTTGCA GGGTGGCATC TTCTCCACCA ATGAAGAGGA TGTTCAGCTC 
GATGGCGATC GTTTGCACGA CGACAACTGT AAGGTATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609189.2
CDS	104..3385
Translation	MRDVPKSLGLSVTTPGGSSGAPDSGRHNSLEEINLDQFLKTSNYEDTVKQLDIYYGIVKRQLLRYQSPITGLFPVMSTDQVVGSVRDSVYCASAVWSLYQAYRRIDDDRGKSYELGQSTVKCMRGILECWVKQASRVELFKQRQSNQHALHSKFQLHTGEKIYPDEFYNHLQIDCVSLYLLFLVQMITSGLQIIYTHDEVAFVQNLVYYVERAYRTPDFGMWERGSKYNNGTPEIHASSIGMAKSALEAINGCNLFGEKGASWSVVYVDIDAHNRNRSIFETMLPRESSSKGVDASLLLTLSFPAFASHEDRLVEQTKQNVVNRLRCKMGFKRFTRDGFLSKNEDKSRRYYHSGELKEFEGLECEWPLFFIAMIIDGVFKNNNEQIEEFQNDLRRCLRTDVNGDPVVTMYYAPDGDGSYMRAPSQSLFLWGQSFFIIAQLLTAGLLHINELDPIRRYLPSYNRPRRAGRYSAFQGTATDLVVQIVLIAESMRLQAMMATYGIQTQTPHEVEPVQIWSSTELIKVYQHLGVNNKVGLSGRPCRPVGSLGTSKVYRICGMTVLCYPLIFEVSDFYLYRDMALLIDDIKTELQFVGKYWRLSGRPTVCLLIREEHMRDPQFKEMLDLLAMLKKGYCDGMKVRIGRLQNLISSSCIEHLDFMNQSDLTDNENAFSQINHEYIGYQSLTDVPKALTYVEEKISVAHFDTKPTPDIINALRSTDSIYCLCQLWGIILNREGPHFEVNGLNVNTALTQLYHRAGSLRYWRAVRYCSSLLHHIVDSISPFITTVLVNGKELTVGIIGQKETVFDKPMTPAEIQNVMYTSVQPYDVIQAVLQQEVVLYCGRLIATNPSMFRGILKIRIGWVLEAMRIYLQISGQQSIDVDNLSPFQVRILLQKVLTVSEWAVEEKLTTLQRRQLEGCLCRVPKHFYNKIWEILQRTPQGILTQGHHLPATPTLTNMSRGELTFNLLVEETLICIDRPERRQITVELLCIVATILNRNPELHFKQALDLDGILAEAFAMYCKDNNIQHQPKPEHEQTKNEDLKAFYSLPYSETTGYLARAAVNKVLQGGIFSTNEEDVQLDGDRLHDDNCKVS

Target ORF information:

RefSeq Version	NM_135345.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8475), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135345.3

ORF Insert Sequence:

ATGCGCGATG TGCCGAAATC TCTCGGTCTC TCGGTCACAA CTCCAGGCGG CAGTTCGGGT 
GCTCCGGACA GTGGTCGCCA CAACAGCTTG GAGGAGATCA ATCTGGACCA GTTCCTGAAA 
ACGTCCAACT ACGAGGACAC GGTGAAGCAG CTGGACATCT ACTATGGCAT TGTCAAGCGT 
CAACTGCTGC GATACCAGAG TCCCATCACC GGTTTGTTTC CGGTGATGAG CACCGACCAG 
GTGGTGGGAT CGGTGCGGGA CAGTGTGTAT TGCGCATCCG CCGTGTGGAG CTTGTACCAG 
GCGTACAGAC GGATCGACGA TGACCGCGGA AAGTCCTACG AACTGGGCCA GAGCACTGTG 
AAGTGCATGC GAGGCATTCT TGAGTGCTGG GTCAAGCAGG CATCGCGGGT GGAGCTCTTT 
AAGCAGCGCC AGTCCAATCA GCACGCACTG CATAGCAAAT TCCAGCTGCA CACCGGAGAG 
AAGATATATC CGGATGAGTT TTACAATCAC CTACAGATAG ATTGCGTTTC TCTCTATCTG 
TTGTTCCTCG TCCAGATGAT AACCTCTGGC CTGCAGATCA TTTACACCCA CGACGAGGTG 
GCCTTTGTCC AGAATCTAGT GTACTATGTG GAGCGCGCCT ATCGTACGCC CGACTTTGGC 
ATGTGGGAGC GGGGCTCTAA ATATAACAAT GGCACCCCGG AGATCCATGC CTCCTCCATT 
GGCATGGCTA AGTCCGCCTT GGAGGCCATT AACGGGTGCA ATCTGTTCGG CGAGAAAGGA 
GCTTCATGGA GCGTTGTCTA TGTGGACATT GATGCCCACA ACCGGAATCG CAGTATTTTC 
GAAACCATGC TGCCCAGGGA ATCAAGCTCA AAGGGAGTTG ATGCTTCACT GCTTCTCACC 
CTTTCTTTCC CAGCCTTTGC CTCGCATGAA GATCGCCTGG TGGAGCAGAC CAAACAAAAT 
GTTGTAAATC GTTTGCGCTG CAAAATGGGC TTTAAGCGCT TCACCCGTGA TGGATTCCTC 
AGCAAAAACG AGGACAAATC GCGACGCTAT TATCACTCTG GCGAGCTAAA GGAATTCGAA 
GGCCTGGAAT GTGAATGGCC GCTCTTTTTC ATAGCCATGA TTATCGATGG CGTGTTCAAG 
AATAACAATG AACAAATTGA AGAGTTCCAA AACGATTTGA GGCGCTGTTT GCGTACTGAT 
GTCAATGGAG ATCCCGTAGT GACCATGTAC TATGCGCCGG ATGGAGATGG CTCCTACATG 
CGTGCTCCAT CGCAATCGCT GTTCCTCTGG GGGCAGTCCT TCTTTATCAT AGCCCAGCTG 
CTGACCGCAG GACTCCTACA CATCAATGAA CTGGATCCAA TCCGCCGCTA CTTGCCAAGC 
TATAATCGGC CTAGAAGGGC GGGTCGTTAT TCGGCTTTCC AGGGCACAGC CACTGATCTT 
GTGGTGCAGA TTGTCCTGAT TGCCGAGTCC ATGCGACTGC AGGCTATGAT GGCCACCTAT 
GGCATTCAAA CACAAACCCC CCATGAGGTG GAACCTGTGC AAATCTGGAG CTCTACAGAG 
CTCATTAAAG TATATCAACA TCTCGGAGTT AACAACAAGG TTGGCCTGTC CGGTCGTCCA 
TGCAGACCGG TGGGTTCTTT GGGCACCAGC AAGGTGTATC GCATCTGCGG CATGACCGTC 
CTCTGCTATC CACTAATCTT TGAGGTCTCC GACTTTTATC TCTACCGGGA CATGGCTCTA 
CTTATCGATG ACATCAAGAC AGAGCTACAG TTTGTGGGCA AGTACTGGCG GCTATCGGGC 
AGACCTACAG TTTGTCTGCT TATTCGCGAG GAGCACATGC GTGATCCGCA GTTTAAGGAG 
ATGCTCGACC TGCTGGCCAT GCTCAAAAAG GGCTACTGCG ATGGCATGAA AGTTCGCATC 
GGTCGTCTAC AGAATCTGAT TAGCAGCTCG TGCATCGAGC ATCTTGACTT TATGAACCAA 
AGTGATCTGA CCGACAACGA GAATGCGTTC TCGCAGATAA ACCATGAGTA TATTGGCTAC 
CAATCGCTAA CAGATGTGCC CAAAGCTCTG ACATATGTGG AGGAAAAGAT TTCTGTTGCG 
CACTTCGATA CCAAACCCAC GCCGGATATC ATCAACGCAC TACGTAGCAC GGATTCCATT 
TACTGTTTGT GCCAATTGTG GGGTATTATT CTCAACCGCG AGGGTCCTCA TTTTGAGGTA 
AACGGTCTGA ACGTAAACAC GGCCTTGACG CAGCTATACC ATCGCGCTGG TTCCTTGCGC 
TACTGGCGTG CCGTGCGATA CTGTTCCTCT CTTCTTCACC ACATTGTGGA CTCCATTAGT 
CCATTTATCA CCACTGTGTT GGTGAATGGC AAGGAGCTCA CAGTGGGCAT CATTGGCCAG 
AAGGAGACGG TATTCGACAA GCCAATGACG CCGGCGGAGA TTCAAAATGT CATGTACACG 
AGTGTCCAAC CCTACGACGT CATCCAGGCA GTGTTGCAGC AGGAAGTGGT TCTGTATTGT 
GGCCGTTTAA TAGCCACCAA TCCATCAATG TTTCGTGGCA TATTAAAAAT TCGCATTGGT 
TGGGTACTGG AGGCCATGCG CATCTATTTA CAAATCTCAG GACAACAGAG CATTGATGTT 
GACAACCTTT CGCCCTTCCA AGTCCGCATA CTTCTTCAGA AAGTCTTAAC CGTCAGCGAA 
TGGGCTGTAG AAGAAAAGCT CACTACCCTA CAACGTCGTC AACTGGAGGG ATGCTTGTGC 
CGTGTGCCAA AACATTTTTA TAACAAAATC TGGGAAATCC TACAGCGAAC CCCTCAAGGC 
ATACTGACCC AGGGGCATCA TTTGCCAGCC ACGCCCACAC TAACCAACAT GAGCCGCGGC 
GAGTTGACCT TCAATCTGCT GGTCGAGGAA ACTCTGATTT GCATTGACCG TCCGGAAAGG 
CGTCAGATAA CCGTGGAGCT TTTGTGCATT GTGGCCACCA TTCTTAATCG TAACCCCGAG 
CTGCACTTTA AGCAAGCTCT AGACTTGGAC GGCATCTTGG CGGAAGCGTT CGCAATGTAC 
TGCAAGGACA ACAACATACA GCATCAACCA AAACCAGAGC ATGAGCAGAC CAAAAATGAG 
GATCTCAAGG CTTTCTATTC GTTGCCATAT TCCGAGACCA CGGGCTATTT GGCTCGCGCC 
GCGGTCAACA AGGTTTTGCA GGGTGGCATC TTCTCCACCA ATGAAGAGGA TGTTCAGCTC 
GATGGCGATC GTTTGCACGA CGACAACTGT AAGGTATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8475 ( NM_001273301.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260230.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG8475), mRNA.

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CloneID	OFa15944
Clone ID Related Accession (Same CDS sequence)	NM_001273301.1
Accession Version	NM_001273301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCGATG TGCCGAAATC TCTCGGTCTC TCGGTCACAA CTCCAGGCGG CAGTTCGGGT 
GCTCCGGACA GTGGTCGCCA CAACAGCTTG GAGGAGATCA ATCTGGACCA GTTCCTGAAA 
ACGTCCAACT ACGAGGACAC GGTGAAGCAG CTGGACATCT ACTATGGCAT TGTCAAGCGT 
CAACTGCTGC GATACCAGAG TCCCATCACC GGTTTGTTTC CGGTGATGAG CACCGACCAG 
GTGGTGGGAT CGGTGCGGGA CAGTGTGTAT TGCGCATCCG CCGTGTGGAG CTTGTACCAG 
GCGTACAGAC GGATCGACGA TGACCGCGGA AAGTCCTACG AACTGGGCCA GAGCACTGTG 
AAGTGCATGC GAGGCATTCT TGAGTGCTGG GTCAAGCAGG CATCGCGGGT GGAGCTCTTT 
AAGCAGCGCC AGTCCAATCA GCACGCACTG CATAGCAAAT TCCAGCTGCA CACCGGAGAG 
AAGATATATC CGGATGAGTT TTACAATCAC CTACAGATAG ATTGCGTTTC TCTCTATCTG 
TTGTTCCTCG TCCAGATGAT AACCTCTGGC CTGCAGATCA TTTACACCCA CGACGAGGTG 
GCCTTTGTCC AGAATCTAGT GTACTATGTG GAGCGCGCCT ATCGTACGCC CGACTTTGGC 
ATGTGGGAGC GGGGCTCTAA ATATAACAAT GGCACCCCGG AGATCCATGC CTCCTCCATT 
GGCATGGCTA AGTCCGCCTT GGAGGCCATT AACGGGTGCA ATCTGTTCGG CGAGAAAGGA 
GCTTCATGGA GCGTTGTCTA TGTGGACATT GATGCCCACA ACCGGAATCG CAGTATTTTC 
GAAACCATGC TGCCCAGGGA ATCAAGCTCA AAGGGAGTTG ATGCTTCACT GCTTCTCACC 
CTTTCTTTCC CAGCCTTTGC CTCGCATGAA GATCGCCTGG TGGAGCAGAC CAAACAAAAT 
GTTGTAAATC GTTTGCGCTG CAAAATGGGC TTTAAGCGCT TCACCCGTGA TGGATTCCTC 
AGCAAAAACG AGGACAAATC GCGACGCTAT TATCACTCTG GCGAGCTAAA GGAATTCGAA 
GGCCTGGAAT GTGAATGGCC GCTCTTTTTC ATAGCCATGA TTATCGATGG CGTGTTCAAG 
AATAACAATG AACAAATTGA AGAGTTCCAA AACGATTTGA GGCGCTGTTT GCGTACTGAT 
GTCAATGGAG ATCCCGTAGT GACCATGTAC TATGCGCCGG ATGGAGATGG CTCCTACATG 
CGTGCTCCAT CGCAATCGCT GTTCCTCTGG GGGCAGTCCT TCTTTATCAT AGCCCAGCTG 
CTGACCGCAG GACTCCTACA CATCAATGAA CTGGATCCAA TCCGCCGCTA CTTGCCAAGC 
TATAATCGGC CTAGAAGGGC GGGTCGTTAT TCGGCTTTCC AGGCTAAACC GGGCACTGGC 
ACAGCCACTG ATCTTGTGGT GCAGATTGTC CTGATTGCCG AGTCCATGCG ACTGCAGGCT 
ATGATGGCCA CCTATGGCAT TCAAACACAA ACCCCCCATG AGGTGGAACC TGTGCAAATC 
TGGAGCTCTA CAGAGCTCAT TAAAGTATAT CAACATCTCG GAGTTAACAA CAAGGTTGGC 
CTGTCCGGTC GTCCATGCAG ACCGGTGGGT TCTTTGGGCA CCAGCAAGGT GTATCGCATC 
TGCGGCATGA CCGTCCTCTG CTATCCACTA ATCTTTGAGG TCTCCGACTT TTATCTCTAC 
CGGGACATGG CTCTACTTAT CGATGACATC AAGACAGAGC TACAGTTTGT GGGCAAGTAC 
TGGCGGCTAT CGGGCAGACC TACAGTTTGT CTGCTTATTC GCGAGGAGCA CATGCGTGAT 
CCGCAGTTTA AGGAGATGCT CGACCTGCTG GCCATGCTCA AAAAGGGCTA CTGCGATGGC 
ATGAAAGTTC GCATCGGTCG TCTACAGAAT CTGATTAGCA GCTCGTGCAT CGAGCATCTT 
GACTTTATGA ACCAAAGTGA TCTGACCGAC AACGAGAATG CGTTCTCGCA GATAAACCAT 
GAGTATATTG GCTACCAATC GCTAACAGAT GTGCCCAAAG CTCTGACATA TGTGGAGGAA 
AAGATTTCTG TTGCGCACTT CGATACCAAA CCCACGCCGG ATATCATCAA CGCACTACGT 
AGCACGGATT CCATTTACTG TTTGTGCCAA TTGTGGGGTA TTATTCTCAA CCGCGAGGGT 
CCTCATTTTG AGGTAAACGG TCTGAACGTA AACACGGCCT TGACGCAGCT ATACCATCGC 
GCTGGTTCCT TGCGCTACTG GCGTGCCGTG CGATACTGTT CCTCTCTTCT TCACCACATT 
GTGGACTCCA TTAGTCCATT TATCACCACT GTGTTGGTGA ATGGCAAGGA GCTCACAGTG 
GGCATCATTG GCCAGAAGGA GACGGTATTC GACAAGCCAA TGACGCCGGC GGAGATTCAA 
AATGTCATGT ACACGAGTGT CCAACCCTAC GACGTCATCC AGGCAGTGTT GCAGCAGGAA 
GTGGTTCTGT ATTGTGGCCG TTTAATAGCC ACCAATCCAT CAATGTTTCG TGGCATATTA 
AAAATTCGCA TTGGTTGGGT ACTGGAGGCC ATGCGCATCT ATTTACAAAT CTCAGGACAA 
CAGAGCATTG ATGTTGACAA CCTTTCGCCC TTCCAAGTCC GCATACTTCT TCAGAAAGTC 
TTAACCGTCA GCGAATGGGC TGTAGAAGAA AAGCTCACTA CCCTACAACG TCGTCAACTG 
GAGGGATGCT TGTGCCGTGT GCCAAAACAT TTTTATAACA AAATCTGGGA AATCCTACAG 
CGAACCCCTC AAGGCATACT GACCCAGGGG CATCATTTGC CAGCCACGCC CACACTAACC 
AACATGAGCC GCGGCGAGTT GACCTTCAAT CTGCTGGTCG AGGAAACTCT GATTTGCATT 
GACCGTCCGG AAAGGCGTCA GATAACCGTG GAGCTTTTGT GCATTGTGGC CACCATTCTT 
AATCGTAACC CCGAGCTGCA CTTTAAGCAA GCTCTAGACT TGGACGGCAT CTTGGCGGAA 
GCGTTCGCAA TGTACTGCAA GGACAACAAC ATACAGCATC AACCAAAACC AGAGCATGAG 
CAGACCAAAA ATGAGGATCT CAAGGCTTTC TATTCGTTGC CATATTCCGA GACCACGGGC 
TATTTGGCTC GCGCCGCGGT CAACAAGGTT TTGCAGGGTG GCATCTTCTC CACCAATGAA 
GAGGATGTTC AGCTCGATGG CGATCGTTTG CACGACGACA ACTGTAAGGT ATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260230.1
CDS	104..3400
Translation	MRDVPKSLGLSVTTPGGSSGAPDSGRHNSLEEINLDQFLKTSNYEDTVKQLDIYYGIVKRQLLRYQSPITGLFPVMSTDQVVGSVRDSVYCASAVWSLYQAYRRIDDDRGKSYELGQSTVKCMRGILECWVKQASRVELFKQRQSNQHALHSKFQLHTGEKIYPDEFYNHLQIDCVSLYLLFLVQMITSGLQIIYTHDEVAFVQNLVYYVERAYRTPDFGMWERGSKYNNGTPEIHASSIGMAKSALEAINGCNLFGEKGASWSVVYVDIDAHNRNRSIFETMLPRESSSKGVDASLLLTLSFPAFASHEDRLVEQTKQNVVNRLRCKMGFKRFTRDGFLSKNEDKSRRYYHSGELKEFEGLECEWPLFFIAMIIDGVFKNNNEQIEEFQNDLRRCLRTDVNGDPVVTMYYAPDGDGSYMRAPSQSLFLWGQSFFIIAQLLTAGLLHINELDPIRRYLPSYNRPRRAGRYSAFQAKPGTGTATDLVVQIVLIAESMRLQAMMATYGIQTQTPHEVEPVQIWSSTELIKVYQHLGVNNKVGLSGRPCRPVGSLGTSKVYRICGMTVLCYPLIFEVSDFYLYRDMALLIDDIKTELQFVGKYWRLSGRPTVCLLIREEHMRDPQFKEMLDLLAMLKKGYCDGMKVRIGRLQNLISSSCIEHLDFMNQSDLTDNENAFSQINHEYIGYQSLTDVPKALTYVEEKISVAHFDTKPTPDIINALRSTDSIYCLCQLWGIILNREGPHFEVNGLNVNTALTQLYHRAGSLRYWRAVRYCSSLLHHIVDSISPFITTVLVNGKELTVGIIGQKETVFDKPMTPAEIQNVMYTSVQPYDVIQAVLQQEVVLYCGRLIATNPSMFRGILKIRIGWVLEAMRIYLQISGQQSIDVDNLSPFQVRILLQKVLTVSEWAVEEKLTTLQRRQLEGCLCRVPKHFYNKIWEILQRTPQGILTQGHHLPATPTLTNMSRGELTFNLLVEETLICIDRPERRQITVELLCIVATILNRNPELHFKQALDLDGILAEAFAMYCKDNNIQHQPKPEHEQTKNEDLKAFYSLPYSETTGYLARAAVNKVLQGGIFSTNEEDVQLDGDRLHDDNCKVS

Target ORF information:

RefSeq Version	NM_001273301.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG8475), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273301.1

ORF Insert Sequence:

ATGCGCGATG TGCCGAAATC TCTCGGTCTC TCGGTCACAA CTCCAGGCGG CAGTTCGGGT 
GCTCCGGACA GTGGTCGCCA CAACAGCTTG GAGGAGATCA ATCTGGACCA GTTCCTGAAA 
ACGTCCAACT ACGAGGACAC GGTGAAGCAG CTGGACATCT ACTATGGCAT TGTCAAGCGT 
CAACTGCTGC GATACCAGAG TCCCATCACC GGTTTGTTTC CGGTGATGAG CACCGACCAG 
GTGGTGGGAT CGGTGCGGGA CAGTGTGTAT TGCGCATCCG CCGTGTGGAG CTTGTACCAG 
GCGTACAGAC GGATCGACGA TGACCGCGGA AAGTCCTACG AACTGGGCCA GAGCACTGTG 
AAGTGCATGC GAGGCATTCT TGAGTGCTGG GTCAAGCAGG CATCGCGGGT GGAGCTCTTT 
AAGCAGCGCC AGTCCAATCA GCACGCACTG CATAGCAAAT TCCAGCTGCA CACCGGAGAG 
AAGATATATC CGGATGAGTT TTACAATCAC CTACAGATAG ATTGCGTTTC TCTCTATCTG 
TTGTTCCTCG TCCAGATGAT AACCTCTGGC CTGCAGATCA TTTACACCCA CGACGAGGTG 
GCCTTTGTCC AGAATCTAGT GTACTATGTG GAGCGCGCCT ATCGTACGCC CGACTTTGGC 
ATGTGGGAGC GGGGCTCTAA ATATAACAAT GGCACCCCGG AGATCCATGC CTCCTCCATT 
GGCATGGCTA AGTCCGCCTT GGAGGCCATT AACGGGTGCA ATCTGTTCGG CGAGAAAGGA 
GCTTCATGGA GCGTTGTCTA TGTGGACATT GATGCCCACA ACCGGAATCG CAGTATTTTC 
GAAACCATGC TGCCCAGGGA ATCAAGCTCA AAGGGAGTTG ATGCTTCACT GCTTCTCACC 
CTTTCTTTCC CAGCCTTTGC CTCGCATGAA GATCGCCTGG TGGAGCAGAC CAAACAAAAT 
GTTGTAAATC GTTTGCGCTG CAAAATGGGC TTTAAGCGCT TCACCCGTGA TGGATTCCTC 
AGCAAAAACG AGGACAAATC GCGACGCTAT TATCACTCTG GCGAGCTAAA GGAATTCGAA 
GGCCTGGAAT GTGAATGGCC GCTCTTTTTC ATAGCCATGA TTATCGATGG CGTGTTCAAG 
AATAACAATG AACAAATTGA AGAGTTCCAA AACGATTTGA GGCGCTGTTT GCGTACTGAT 
GTCAATGGAG ATCCCGTAGT GACCATGTAC TATGCGCCGG ATGGAGATGG CTCCTACATG 
CGTGCTCCAT CGCAATCGCT GTTCCTCTGG GGGCAGTCCT TCTTTATCAT AGCCCAGCTG 
CTGACCGCAG GACTCCTACA CATCAATGAA CTGGATCCAA TCCGCCGCTA CTTGCCAAGC 
TATAATCGGC CTAGAAGGGC GGGTCGTTAT TCGGCTTTCC AGGCTAAACC GGGCACTGGC 
ACAGCCACTG ATCTTGTGGT GCAGATTGTC CTGATTGCCG AGTCCATGCG ACTGCAGGCT 
ATGATGGCCA CCTATGGCAT TCAAACACAA ACCCCCCATG AGGTGGAACC TGTGCAAATC 
TGGAGCTCTA CAGAGCTCAT TAAAGTATAT CAACATCTCG GAGTTAACAA CAAGGTTGGC 
CTGTCCGGTC GTCCATGCAG ACCGGTGGGT TCTTTGGGCA CCAGCAAGGT GTATCGCATC 
TGCGGCATGA CCGTCCTCTG CTATCCACTA ATCTTTGAGG TCTCCGACTT TTATCTCTAC 
CGGGACATGG CTCTACTTAT CGATGACATC AAGACAGAGC TACAGTTTGT GGGCAAGTAC 
TGGCGGCTAT CGGGCAGACC TACAGTTTGT CTGCTTATTC GCGAGGAGCA CATGCGTGAT 
CCGCAGTTTA AGGAGATGCT CGACCTGCTG GCCATGCTCA AAAAGGGCTA CTGCGATGGC 
ATGAAAGTTC GCATCGGTCG TCTACAGAAT CTGATTAGCA GCTCGTGCAT CGAGCATCTT 
GACTTTATGA ACCAAAGTGA TCTGACCGAC AACGAGAATG CGTTCTCGCA GATAAACCAT 
GAGTATATTG GCTACCAATC GCTAACAGAT GTGCCCAAAG CTCTGACATA TGTGGAGGAA 
AAGATTTCTG TTGCGCACTT CGATACCAAA CCCACGCCGG ATATCATCAA CGCACTACGT 
AGCACGGATT CCATTTACTG TTTGTGCCAA TTGTGGGGTA TTATTCTCAA CCGCGAGGGT 
CCTCATTTTG AGGTAAACGG TCTGAACGTA AACACGGCCT TGACGCAGCT ATACCATCGC 
GCTGGTTCCT TGCGCTACTG GCGTGCCGTG CGATACTGTT CCTCTCTTCT TCACCACATT 
GTGGACTCCA TTAGTCCATT TATCACCACT GTGTTGGTGA ATGGCAAGGA GCTCACAGTG 
GGCATCATTG GCCAGAAGGA GACGGTATTC GACAAGCCAA TGACGCCGGC GGAGATTCAA 
AATGTCATGT ACACGAGTGT CCAACCCTAC GACGTCATCC AGGCAGTGTT GCAGCAGGAA 
GTGGTTCTGT ATTGTGGCCG TTTAATAGCC ACCAATCCAT CAATGTTTCG TGGCATATTA 
AAAATTCGCA TTGGTTGGGT ACTGGAGGCC ATGCGCATCT ATTTACAAAT CTCAGGACAA 
CAGAGCATTG ATGTTGACAA CCTTTCGCCC TTCCAAGTCC GCATACTTCT TCAGAAAGTC 
TTAACCGTCA GCGAATGGGC TGTAGAAGAA AAGCTCACTA CCCTACAACG TCGTCAACTG 
GAGGGATGCT TGTGCCGTGT GCCAAAACAT TTTTATAACA AAATCTGGGA AATCCTACAG 
CGAACCCCTC AAGGCATACT GACCCAGGGG CATCATTTGC CAGCCACGCC CACACTAACC 
AACATGAGCC GCGGCGAGTT GACCTTCAAT CTGCTGGTCG AGGAAACTCT GATTTGCATT 
GACCGTCCGG AAAGGCGTCA GATAACCGTG GAGCTTTTGT GCATTGTGGC CACCATTCTT 
AATCGTAACC CCGAGCTGCA CTTTAAGCAA GCTCTAGACT TGGACGGCAT CTTGGCGGAA 
GCGTTCGCAA TGTACTGCAA GGACAACAAC ATACAGCATC AACCAAAACC AGAGCATGAG 
CAGACCAAAA ATGAGGATCT CAAGGCTTTC TATTCGTTGC CATATTCCGA GACCACGGGC 
TATTTGGCTC GCGCCGCGGT CAACAAGGTT TTGCAGGGTG GCATCTTCTC CACCAATGAA 
GAGGATGTTC AGCTCGATGG CGATCGTTTG CACGACGACA ACTGTAAGGT ATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG8475 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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