PAPLA1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PAPLA1
Entrez Gene ID	34109
Full Name	Phosphatidic Acid Phospholipase A1
Synonyms	CG14278,CG8552,Dmel\CG8552,Sec23-IP
General protein information	Preferred Names Phosphatidic Acid Phospholipase A1 Names CG8552 gene product from transcript CG8552-RD CG8552-PA CG8552-PB CG8552-PD CG8552-PE PAPLA1-PA PAPLA1-PB PAPLA1-PD PAPLA1-PE Sec23-interacting protein phosphatidic acid phospholipase A1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 28E7-28E9

mRNA and Protein(s)

mRNA	Protein	Name
NM_001201785.1	NP_001188714.1	phosphatidic acid phospholipase A1, isoform B
NM_001298812.1	NP_001285741.1	phosphatidic acid phospholipase A1, isoform D
NM_135341.1	NP_609185.1	phosphatidic acid phospholipase A1, isoform A
NM_001201786.2	NP_001188715.1	phosphatidic acid phospholipase A1, isoform E

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DDHD2	XP_519711.3
Canis lupus familiaris (dog)	DDHD2	XP_532805.3
Rattus norvegicus (Norway rat)	Ddhd2	XP_006253401.1
Homo sapiens (human)	DDHD2	NP_056029.2
	DDHD2	XP_001092630.2
Bos taurus (cattle)	DDHD2	NP_001069066.2
Gallus gallus (chicken)	DDHD2	XP_424389.3
Xenopus tropicalis (tropical clawed frog)	ddhd2	XP_002932759.2
Danio rerio (zebrafish)	ddhd2	XP_002663587.3
Mus musculus (house mouse)	Ddhd2	NP_082378.1
Drosophila melanogaster (fruit fly)	CG8552	NP_001188714.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002379	XP_001237480.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Drosophila proteinin CG8552 is a phosphatidic acid phospholipase A1 and mediates endoplasmic reticulum-Golgi transit of a family of G protein-coupled receptors.
Title: Phosphatidic acid phospholipase A1 mediates ER-Golgi transit of a family of G protein-coupled receptors.

The following PAPLA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PAPLA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15936	NM_001201785.1 Latest version!	Drosophila melanogaster phosphatidic acid phospholipase A1, transcript variant B (PAPLA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15936	NM_001201786.2 Latest version!	Drosophila melanogaster phosphatidic acid phospholipase A1, transcript variant E (PAPLA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14086	NM_135341.1 Latest version!	Drosophila melanogaster phosphatidic acid phospholipase A1, transcript variant A (PAPLA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa21617	NM_001298812.1 Latest version!	Drosophila melanogaster phosphatidic acid phospholipase A1, transcript variant D (PAPLA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PAPLA1 ( NM_001201785.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188714.1 Drosophila melanogaster phosphatidic acid phospholipase A1, transcript variant B (PAPLA1), mRNA.

Price & Availability↑

CloneID	OFa15936
Clone ID Related Accession (Same CDS sequence)	NM_001201785.1 , NM_001201786.2
Accession Version	NM_001201785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatidic acid phospholipase A1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAAGTG CCAGCAGCGA AAAGGGCCAG GTGGCGACGC CTCCCGTGGC TATACCTTAC 
TCACCAGTGG TGGCCCATTG GTTCTACAAG CGCAGCGTGG ACACAAAGTT CATCTGGACA 
CCATTCAGCC ACTATGATTC GGCCTTGTTA GAAACCAGCC TAAATCTTGA CGATTCCACA 
TTGATTATTC CCGTTGAGGG TGGCCGCTAC GATGTAAACA TCAAGGAACG TACCAAGACT 
CCCGTTTACT GGGAGGGAAA GGCCATCGAA GTGCGACGCT GCTCCTGGTT CTACAAGGGA 
GTTGACTCCA AGTATGTTCC ATACACCGAG GATACGGCTG CACTACTTGA AGCGGAATAC 
AAGCGATCTG CGGAGACTGG TGAATGGCAT CAGAAGATTA TGCTGGCCAA TGGCGAACAG 
GTGGTCTTCC ACGGACCTAC AGTCATCGTT CACTTCCTGC CGCAACAGAA TGCAGACACT 
TGGGGAGCCA GCACACAGGG TTCCATGCGT CCGAGAGTTG TCAAGAGGGA TCTGGATGAT 
TTCACCATCG AGCAAGGCGA ATCGCAGCGA GTCGATCACC TGCTTTTCAT GGTCCATGGC 
ATTGGCTCAG CCTGCGATTT GAAAATGCGT TCCGTGGAGG AAGTGGTGGA TGACTTCCGG 
GTTATTGCCC AGCAACTAGT TCAGTCGCAC TACAAAAACT CAACAGATAT GGGCCTGGTG 
GGTCGCGTTG AAGTTTTGCC CATCTCGTGG CACGGACACC TGCACTCCGA GGAGTTAGGC 
ATCGACGAAA AGCTGAAATC CATCACTTTG GAGTCTATTC CTCGCTTGCG AAACTTCACC 
AATGACACGC TGCTCGACGT GCTGTTCTAC ACCAGTCCGA AATACTGTCA AAAGATCATG 
AACACGGTGG CAGATGCGCT TAACGATGTC TACCTGAAGT ACCGAATGCG GCACCCTGAA 
TTCAATGGGG GAGTTTCGCT GGCAGGTCAC AGTCTCGGCT CCCTAATCCT ATTCGATTTA 
TTGTGCCATC AGGAACCGCT CAAGGAAAGC GAGGAAGAGA ATAAAGAGAA TCCCGACCAA 
CTTCCCCAAA AGCAATCGAG TCAAAAGGTA CAGCTTCCAA CCAGCGATCC TTTGCCGAAA 
CAAGTCAGCT ATGCGATGGG TCCGGAGGGC ACGGGACAGC CCGTGATTAC ATATACACAG 
CTGATATTCC ACCCCAAAAA ATTCTTTGCT CTGGGCTCAC CCATCGGAAT GTTTGTAACC 
ATCAGGGGGA TTGATAAGCT TGGTCTCGAC TTTCACCTGC CCACGTGTCC GGGTTTCTAC 
AACATATTCC ATCCCTTCGA TCCGGTAGCC TATCGGATCG AGGCGCTTGT CAATCCGGAC 
ATGAACGGCA TTCGACCGGT GTTAATTCCC CATCACAAGG GACGAAAACG AATGCACCTG 
GAGCTGAAGG AGACGATGAC ACGTGTGGGT GCGGACATCA AGCAGCGATT TATGGACACA 
TTCAAGACCA CGCTGGACAG CGTAAACTTT TTGACCACGG TAACGAAGGT TAAGAAGGAA 
GCGGAGGAGT CGCTGGAGAA GGAGACAAGC CAGACTTCAT CGCAAGCTTT TCAAAAGCAA 
ACCGAGGATC AGGACGAGAG TTCAGTTGCC AGTTCATCTT GCAAACTACG AAATCGCACC 
GATTCCAATT CGACGACGGC TTCTGACCCT GAGTTTATAG AACTTGATTT CCCACTGGGT 
AAGCTGAACG ACTCAAAACG TGTGGACTAC GTGCTTCAGG AAGCTCCACT AGAATTCATC 
AACGAATATA TTTTCGCCCT GAGCAGCCAT GTGTGCTACT GGGGCTCCGA AGATACGATC 
CTTTTCGTGA TGAAGGAGAT TTATGCCAGC CTGGGAATCA GCACAGACAG CCAGGTGCCG 
CAGTCCTCAA TGACCATCGA GCGACCCGTT TCGCATGAGA GCAGTGTTAG CCACTCGCTG 
TTGCCGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188714.1
CDS	4076..6067
Translation	MASASSEKGQVATPPVAIPYSPVVAHWFYKRSVDTKFIWTPFSHYDSALLETSLNLDDSTLIIPVEGGRYDVNIKERTKTPVYWEGKAIEVRRCSWFYKGVDSKYVPYTEDTAALLEAEYKRSAETGEWHQKIMLANGEQVVFHGPTVIVHFLPQQNADTWGASTQGSMRPRVVKRDLDDFTIEQGESQRVDHLLFMVHGIGSACDLKMRSVEEVVDDFRVIAQQLVQSHYKNSTDMGLVGRVEVLPISWHGHLHSEELGIDEKLKSITLESIPRLRNFTNDTLLDVLFYTSPKYCQKIMNTVADALNDVYLKYRMRHPEFNGGVSLAGHSLGSLILFDLLCHQEPLKESEEENKENPDQLPQKQSSQKVQLPTSDPLPKQVSYAMGPEGTGQPVITYTQLIFHPKKFFALGSPIGMFVTIRGIDKLGLDFHLPTCPGFYNIFHPFDPVAYRIEALVNPDMNGIRPVLIPHHKGRKRMHLELKETMTRVGADIKQRFMDTFKTTLDSVNFLTTVTKVKKEAEESLEKETSQTSSQAFQKQTEDQDESSVASSSCKLRNRTDSNSTTASDPEFIELDFPLGKLNDSKRVDYVLQEAPLEFINEYIFALSSHVCYWGSEDTILFVMKEIYASLGISTDSQVPQSSMTIERPVSHESSVSHSLLPM

Target ORF information:

RefSeq Version	NM_001201785.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatidic acid phospholipase A1, transcript variant B (PAPLA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201785.1

ORF Insert Sequence:

ATGGCAAGTG CCAGCAGCGA AAAGGGCCAG GTGGCGACGC CTCCCGTGGC TATACCTTAC 
TCACCAGTGG TGGCCCATTG GTTCTACAAG CGCAGCGTGG ACACAAAGTT CATCTGGACA 
CCATTCAGCC ACTATGATTC GGCCTTGTTA GAAACCAGCC TAAATCTTGA CGATTCCACA 
TTGATTATTC CCGTTGAGGG TGGCCGCTAC GATGTAAACA TCAAGGAACG TACCAAGACT 
CCCGTTTACT GGGAGGGAAA GGCCATCGAA GTGCGACGCT GCTCCTGGTT CTACAAGGGA 
GTTGACTCCA AGTATGTTCC ATACACCGAG GATACGGCTG CACTACTTGA AGCGGAATAC 
AAGCGATCTG CGGAGACTGG TGAATGGCAT CAGAAGATTA TGCTGGCCAA TGGCGAACAG 
GTGGTCTTCC ACGGACCTAC AGTCATCGTT CACTTCCTGC CGCAACAGAA TGCAGACACT 
TGGGGAGCCA GCACACAGGG TTCCATGCGT CCGAGAGTTG TCAAGAGGGA TCTGGATGAT 
TTCACCATCG AGCAAGGCGA ATCGCAGCGA GTCGATCACC TGCTTTTCAT GGTCCATGGC 
ATTGGCTCAG CCTGCGATTT GAAAATGCGT TCCGTGGAGG AAGTGGTGGA TGACTTCCGG 
GTTATTGCCC AGCAACTAGT TCAGTCGCAC TACAAAAACT CAACAGATAT GGGCCTGGTG 
GGTCGCGTTG AAGTTTTGCC CATCTCGTGG CACGGACACC TGCACTCCGA GGAGTTAGGC 
ATCGACGAAA AGCTGAAATC CATCACTTTG GAGTCTATTC CTCGCTTGCG AAACTTCACC 
AATGACACGC TGCTCGACGT GCTGTTCTAC ACCAGTCCGA AATACTGTCA AAAGATCATG 
AACACGGTGG CAGATGCGCT TAACGATGTC TACCTGAAGT ACCGAATGCG GCACCCTGAA 
TTCAATGGGG GAGTTTCGCT GGCAGGTCAC AGTCTCGGCT CCCTAATCCT ATTCGATTTA 
TTGTGCCATC AGGAACCGCT CAAGGAAAGC GAGGAAGAGA ATAAAGAGAA TCCCGACCAA 
CTTCCCCAAA AGCAATCGAG TCAAAAGGTA CAGCTTCCAA CCAGCGATCC TTTGCCGAAA 
CAAGTCAGCT ATGCGATGGG TCCGGAGGGC ACGGGACAGC CCGTGATTAC ATATACACAG 
CTGATATTCC ACCCCAAAAA ATTCTTTGCT CTGGGCTCAC CCATCGGAAT GTTTGTAACC 
ATCAGGGGGA TTGATAAGCT TGGTCTCGAC TTTCACCTGC CCACGTGTCC GGGTTTCTAC 
AACATATTCC ATCCCTTCGA TCCGGTAGCC TATCGGATCG AGGCGCTTGT CAATCCGGAC 
ATGAACGGCA TTCGACCGGT GTTAATTCCC CATCACAAGG GACGAAAACG AATGCACCTG 
GAGCTGAAGG AGACGATGAC ACGTGTGGGT GCGGACATCA AGCAGCGATT TATGGACACA 
TTCAAGACCA CGCTGGACAG CGTAAACTTT TTGACCACGG TAACGAAGGT TAAGAAGGAA 
GCGGAGGAGT CGCTGGAGAA GGAGACAAGC CAGACTTCAT CGCAAGCTTT TCAAAAGCAA 
ACCGAGGATC AGGACGAGAG TTCAGTTGCC AGTTCATCTT GCAAACTACG AAATCGCACC 
GATTCCAATT CGACGACGGC TTCTGACCCT GAGTTTATAG AACTTGATTT CCCACTGGGT 
AAGCTGAACG ACTCAAAACG TGTGGACTAC GTGCTTCAGG AAGCTCCACT AGAATTCATC 
AACGAATATA TTTTCGCCCT GAGCAGCCAT GTGTGCTACT GGGGCTCCGA AGATACGATC 
CTTTTCGTGA TGAAGGAGAT TTATGCCAGC CTGGGAATCA GCACAGACAG CCAGGTGCCG 
CAGTCCTCAA TGACCATCGA GCGACCCGTT TCGCATGAGA GCAGTGTTAG CCACTCGCTG 
TTGCCGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPLA1 ( NM_001201786.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188715.1 Drosophila melanogaster phosphatidic acid phospholipase A1, transcript variant E (PAPLA1), mRNA.

Price & Availability↑

CloneID	OFa15936
Clone ID Related Accession (Same CDS sequence)	NM_001201785.1 , NM_001201786.2
Accession Version	NM_001201786.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatidic acid phospholipase A1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001201786.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAAGTG CCAGCAGCGA AAAGGGCCAG GTGGCGACGC CTCCCGTGGC TATACCTTAC 
TCACCAGTGG TGGCCCATTG GTTCTACAAG CGCAGCGTGG ACACAAAGTT CATCTGGACA 
CCATTCAGCC ACTATGATTC GGCCTTGTTA GAAACCAGCC TAAATCTTGA CGATTCCACA 
TTGATTATTC CCGTTGAGGG TGGCCGCTAC GATGTAAACA TCAAGGAACG TACCAAGACT 
CCCGTTTACT GGGAGGGAAA GGCCATCGAA GTGCGACGCT GCTCCTGGTT CTACAAGGGA 
GTTGACTCCA AGTATGTTCC ATACACCGAG GATACGGCTG CACTACTTGA AGCGGAATAC 
AAGCGATCTG CGGAGACTGG TGAATGGCAT CAGAAGATTA TGCTGGCCAA TGGCGAACAG 
GTGGTCTTCC ACGGACCTAC AGTCATCGTT CACTTCCTGC CGCAACAGAA TGCAGACACT 
TGGGGAGCCA GCACACAGGG TTCCATGCGT CCGAGAGTTG TCAAGAGGGA TCTGGATGAT 
TTCACCATCG AGCAAGGCGA ATCGCAGCGA GTCGATCACC TGCTTTTCAT GGTCCATGGC 
ATTGGCTCAG CCTGCGATTT GAAAATGCGT TCCGTGGAGG AAGTGGTGGA TGACTTCCGG 
GTTATTGCCC AGCAACTAGT TCAGTCGCAC TACAAAAACT CAACAGATAT GGGCCTGGTG 
GGTCGCGTTG AAGTTTTGCC CATCTCGTGG CACGGACACC TGCACTCCGA GGAGTTAGGC 
ATCGACGAAA AGCTGAAATC CATCACTTTG GAGTCTATTC CTCGCTTGCG AAACTTCACC 
AATGACACGC TGCTCGACGT GCTGTTCTAC ACCAGTCCGA AATACTGTCA AAAGATCATG 
AACACGGTGG CAGATGCGCT TAACGATGTC TACCTGAAGT ACCGAATGCG GCACCCTGAA 
TTCAATGGGG GAGTTTCGCT GGCAGGTCAC AGTCTCGGCT CCCTAATCCT ATTCGATTTA 
TTGTGCCATC AGGAACCGCT CAAGGAAAGC GAGGAAGAGA ATAAAGAGAA TCCCGACCAA 
CTTCCCCAAA AGCAATCGAG TCAAAAGGTA CAGCTTCCAA CCAGCGATCC TTTGCCGAAA 
CAAGTCAGCT ATGCGATGGG TCCGGAGGGC ACGGGACAGC CCGTGATTAC ATATACACAG 
CTGATATTCC ACCCCAAAAA ATTCTTTGCT CTGGGCTCAC CCATCGGAAT GTTTGTAACC 
ATCAGGGGGA TTGATAAGCT TGGTCTCGAC TTTCACCTGC CCACGTGTCC GGGTTTCTAC 
AACATATTCC ATCCCTTCGA TCCGGTAGCC TATCGGATCG AGGCGCTTGT CAATCCGGAC 
ATGAACGGCA TTCGACCGGT GTTAATTCCC CATCACAAGG GACGAAAACG AATGCACCTG 
GAGCTGAAGG AGACGATGAC ACGTGTGGGT GCGGACATCA AGCAGCGATT TATGGACACA 
TTCAAGACCA CGCTGGACAG CGTAAACTTT TTGACCACGG TAACGAAGGT TAAGAAGGAA 
GCGGAGGAGT CGCTGGAGAA GGAGACAAGC CAGACTTCAT CGCAAGCTTT TCAAAAGCAA 
ACCGAGGATC AGGACGAGAG TTCAGTTGCC AGTTCATCTT GCAAACTACG AAATCGCACC 
GATTCCAATT CGACGACGGC TTCTGACCCT GAGTTTATAG AACTTGATTT CCCACTGGGT 
AAGCTGAACG ACTCAAAACG TGTGGACTAC GTGCTTCAGG AAGCTCCACT AGAATTCATC 
AACGAATATA TTTTCGCCCT GAGCAGCCAT GTGTGCTACT GGGGCTCCGA AGATACGATC 
CTTTTCGTGA TGAAGGAGAT TTATGCCAGC CTGGGAATCA GCACAGACAG CCAGGTGCCG 
CAGTCCTCAA TGACCATCGA GCGACCCGTT TCGCATGAGA GCAGTGTTAG CCACTCGCTG 
TTGCCGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188715.1
CDS	3979..5970
Translation	MASASSEKGQVATPPVAIPYSPVVAHWFYKRSVDTKFIWTPFSHYDSALLETSLNLDDSTLIIPVEGGRYDVNIKERTKTPVYWEGKAIEVRRCSWFYKGVDSKYVPYTEDTAALLEAEYKRSAETGEWHQKIMLANGEQVVFHGPTVIVHFLPQQNADTWGASTQGSMRPRVVKRDLDDFTIEQGESQRVDHLLFMVHGIGSACDLKMRSVEEVVDDFRVIAQQLVQSHYKNSTDMGLVGRVEVLPISWHGHLHSEELGIDEKLKSITLESIPRLRNFTNDTLLDVLFYTSPKYCQKIMNTVADALNDVYLKYRMRHPEFNGGVSLAGHSLGSLILFDLLCHQEPLKESEEENKENPDQLPQKQSSQKVQLPTSDPLPKQVSYAMGPEGTGQPVITYTQLIFHPKKFFALGSPIGMFVTIRGIDKLGLDFHLPTCPGFYNIFHPFDPVAYRIEALVNPDMNGIRPVLIPHHKGRKRMHLELKETMTRVGADIKQRFMDTFKTTLDSVNFLTTVTKVKKEAEESLEKETSQTSSQAFQKQTEDQDESSVASSSCKLRNRTDSNSTTASDPEFIELDFPLGKLNDSKRVDYVLQEAPLEFINEYIFALSSHVCYWGSEDTILFVMKEIYASLGISTDSQVPQSSMTIERPVSHESSVSHSLLPM

Target ORF information:

RefSeq Version	NM_001201786.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatidic acid phospholipase A1, transcript variant E (PAPLA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201786.2

ORF Insert Sequence:

ATGGCAAGTG CCAGCAGCGA AAAGGGCCAG GTGGCGACGC CTCCCGTGGC TATACCTTAC 
TCACCAGTGG TGGCCCATTG GTTCTACAAG CGCAGCGTGG ACACAAAGTT CATCTGGACA 
CCATTCAGCC ACTATGATTC GGCCTTGTTA GAAACCAGCC TAAATCTTGA CGATTCCACA 
TTGATTATTC CCGTTGAGGG TGGCCGCTAC GATGTAAACA TCAAGGAACG TACCAAGACT 
CCCGTTTACT GGGAGGGAAA GGCCATCGAA GTGCGACGCT GCTCCTGGTT CTACAAGGGA 
GTTGACTCCA AGTATGTTCC ATACACCGAG GATACGGCTG CACTACTTGA AGCGGAATAC 
AAGCGATCTG CGGAGACTGG TGAATGGCAT CAGAAGATTA TGCTGGCCAA TGGCGAACAG 
GTGGTCTTCC ACGGACCTAC AGTCATCGTT CACTTCCTGC CGCAACAGAA TGCAGACACT 
TGGGGAGCCA GCACACAGGG TTCCATGCGT CCGAGAGTTG TCAAGAGGGA TCTGGATGAT 
TTCACCATCG AGCAAGGCGA ATCGCAGCGA GTCGATCACC TGCTTTTCAT GGTCCATGGC 
ATTGGCTCAG CCTGCGATTT GAAAATGCGT TCCGTGGAGG AAGTGGTGGA TGACTTCCGG 
GTTATTGCCC AGCAACTAGT TCAGTCGCAC TACAAAAACT CAACAGATAT GGGCCTGGTG 
GGTCGCGTTG AAGTTTTGCC CATCTCGTGG CACGGACACC TGCACTCCGA GGAGTTAGGC 
ATCGACGAAA AGCTGAAATC CATCACTTTG GAGTCTATTC CTCGCTTGCG AAACTTCACC 
AATGACACGC TGCTCGACGT GCTGTTCTAC ACCAGTCCGA AATACTGTCA AAAGATCATG 
AACACGGTGG CAGATGCGCT TAACGATGTC TACCTGAAGT ACCGAATGCG GCACCCTGAA 
TTCAATGGGG GAGTTTCGCT GGCAGGTCAC AGTCTCGGCT CCCTAATCCT ATTCGATTTA 
TTGTGCCATC AGGAACCGCT CAAGGAAAGC GAGGAAGAGA ATAAAGAGAA TCCCGACCAA 
CTTCCCCAAA AGCAATCGAG TCAAAAGGTA CAGCTTCCAA CCAGCGATCC TTTGCCGAAA 
CAAGTCAGCT ATGCGATGGG TCCGGAGGGC ACGGGACAGC CCGTGATTAC ATATACACAG 
CTGATATTCC ACCCCAAAAA ATTCTTTGCT CTGGGCTCAC CCATCGGAAT GTTTGTAACC 
ATCAGGGGGA TTGATAAGCT TGGTCTCGAC TTTCACCTGC CCACGTGTCC GGGTTTCTAC 
AACATATTCC ATCCCTTCGA TCCGGTAGCC TATCGGATCG AGGCGCTTGT CAATCCGGAC 
ATGAACGGCA TTCGACCGGT GTTAATTCCC CATCACAAGG GACGAAAACG AATGCACCTG 
GAGCTGAAGG AGACGATGAC ACGTGTGGGT GCGGACATCA AGCAGCGATT TATGGACACA 
TTCAAGACCA CGCTGGACAG CGTAAACTTT TTGACCACGG TAACGAAGGT TAAGAAGGAA 
GCGGAGGAGT CGCTGGAGAA GGAGACAAGC CAGACTTCAT CGCAAGCTTT TCAAAAGCAA 
ACCGAGGATC AGGACGAGAG TTCAGTTGCC AGTTCATCTT GCAAACTACG AAATCGCACC 
GATTCCAATT CGACGACGGC TTCTGACCCT GAGTTTATAG AACTTGATTT CCCACTGGGT 
AAGCTGAACG ACTCAAAACG TGTGGACTAC GTGCTTCAGG AAGCTCCACT AGAATTCATC 
AACGAATATA TTTTCGCCCT GAGCAGCCAT GTGTGCTACT GGGGCTCCGA AGATACGATC 
CTTTTCGTGA TGAAGGAGAT TTATGCCAGC CTGGGAATCA GCACAGACAG CCAGGTGCCG 
CAGTCCTCAA TGACCATCGA GCGACCCGTT TCGCATGAGA GCAGTGTTAG CCACTCGCTG 
TTGCCGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPLA1 ( NM_135341.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609185.1 Drosophila melanogaster phosphatidic acid phospholipase A1, transcript variant A (PAPLA1), mRNA.

Price & Availability↑

CloneID	OFa14086
Clone ID Related Accession (Same CDS sequence)	NM_135341.1
Accession Version	NM_135341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatidic acid phospholipase A1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAAA GATCGAAAGG TGACGTGTCC GCTCGCGTAA ACAACAACGC AAACCAGACG 
CGTGTCGTTA AAAATCCGCT TCTGAGCGCC TCCGTGACCG GCCTGAGTTT CGACGACTTC 
CCCAACAAGC CGCAGCTCCT GCCAGCCGCC CCGCCCGTGG TCCATGCCAC GCCTCCAGCG 
GCGCCCGTCC TCATCGCTGG CATCTTGAAT CGCGAACCGA AATCATTGGT CACCGTTGGT 
CAGCCATCGA GCATTGACGA TAGCGAGGCG CTCGACGAGA TTAACTTGAA TACCAGCAGC 
GAGGATTCCG TTGGCGCAGG TGGATCGAGT CCATATCTAG GAGCAGGCGA TCAAAACGCC 
AATCCTCTGT TGGAACCACC AACGGAAGCT GGAAACGATT CGTTGCTGAG CCAGGCGGCC 
TCCTCCTTTA CTGCCCTGCC TGCGGTGGCA TCCAATGTGT TTTCCACCTT CTCGAAGCGG 
ATCTACGCCG GCAGCAGGGA GACCTCGGAG GAGCCCAAAC TAGACATACA GCCCTCTTAC 
ATTCAGCAGG CCAGCTACGT TCAGCCAGTA GCTCCAGTGC CAGCTCCCTT TTATGCAGCA 
CCGCCAGCGG CCGCAAACGA AGTGGTCGCC CCCGAACCAC CCAAGTTCTA CGCACCTACT 
GAGGTTCCGC CACCGAATGT AGGAGTCCCT GCACCACCGC CTACATCGGG AAATCCAAAC 
ACATACAGAT TCACCGCCAG GAAGAAGCTA TACGCTCCCA TTCCTGGATT ATCCGAGCAG 
TCCGGACAAC CTGCACAACA GCCACAGGTT CCTCAGGCTC CCCAAGCGGC TCCATCATTC 
CAGACGCCAC CATATCCACC ACAGCTAACG TACGCAGCAA ACCCTGCTCC CTTTGACATA 
TCAGCGCAGC CAGCTGCAAT TCCCGCAGAG GAACGCAAAT CTGGTGGAGG ACTCTTCTCT 
TTGACCAGTC TCGTGCCCAC AGGAGTGCTT CAAAATATCA CAGGAATTGT TCAATCAGCC 
ACTGGGCGAA GCAGTGAACC AGAACACACT AACGTCCAGC AAGCAGGAGG CTACCTAGAC 
ATTACAACAG CATCAGCACC AGCTCCATCG ACCAACTTAT TTGGTGGACC GAATTCAACC 
TTTCCGCCGG GAGGAAATTA TTTCGTACCG GGAGCACCTC CATCATTACC CTCAACAGGG 
GTAGTAGGCC CCCCTTCGGT CGGAAACTTC TTCGCGCCGG CTGCTCCATT GCCAGTAGCA 
CAAACAGCTG CTCCACCAGC TGTCGGAGCC TTCTTCACAT CTGCTGCCCC AAGTGTTACG 
CCTTCAGTTT TTACTCCAGG AACAGTTCCA CCTCTTGCAC CGCAGCCTTT TCAGTCAGCA 
ACTGTAAACC AATCTGCATT GCCACCAAAC AATCCGCCGA CCGCATCTGC AGTAGGAGAA 
CTCTTTGTTC CAGCACCAAC GACAATCGCC CAAGGAACTT CACCTGCAGT TCCATTTGTT 
GACACACAAG CTGTTCCAAC AATACCTTTA ACAGTTCCAC CAGGCAATCC TCCTCCAACA 
TCGACACTTG GAGGATTCTT TACCACAGGT GAAGTTGCAC CTGTTGTTGC GCCATTTGGA 
ACACAAGCGT CACCAGCAAC ACAATCTGCA GGATTCTTTA ATCCAGGAGA AGTTCGTCCT 
AATGTCCCGT TAGCCGAACC TGTTCCAACT ATACCGATTA CATCATCAGG TGTTCCACCT 
ATAGGATTAT TTCCTCCAGC ACCGGCACAG CCTGGTGTCC AAACTGCTTC AAATCCGAAT 
CCCTTGCCAG GAGCAGCTGC ACCGCCACCA GCCGCTGGGC AAGCTTCATA TCGTCTCCAG 
AAGGGAACTC GTCTTTACAA GAGTCCATTG ACGCCCCAGG AAACCGCAAC ATCTTCGGGA 
TTCGCCGCTC CTCTGCCCCC CACATCTGTA ACTCCAGCAA TTTTTAATCC ATTCGGAGCA 
CCGGCACCAG GATCTTTCAA TTCAGTAGGC CATCTAGAGG GTCAATCCAG CCAAGGAGTT 
CCTTTCTTTA CGCCGCCAGT GCAGAATCCA CCAGGACAAG TAGCGGCTCC AACTGTTGCT 
GTTCCGCAAT CAGCACCTAT TGCCCCTCCA CCAGGGACAT CAAGCACAAG TGTTGCTCCT 
CCGCTAACAG CATCTATAGC CAATCCTCCT CCAACATCTG GCTTTGCAAG TGTACCCCTC 
TTCGCTGCAC CAACAATATC CTCAACGCCT ATTCCTTTCT TCAATCCTCA ACCAACAGGA 
GCAGAAGTAA CAACTGGTTT GAGCCAACAG GAAAACGAAG CTACAGCATC GACAACTTTG 
GAAGGTCTAG TGCAAGGACC TGCTCCAACA CTCTCTGCAA GTCAATTCTT TGATACGCCC 
TTGCCAACAG GAACCCAACC GGAGAATCAA GTAGCAAGTG TTTCCTCGGA AAGAGAAAAT 
CTTGTACAGG AGCCAATATC GAACCAAGGA ATTCCATTCT TTGCGCCGCC GCAAACGAAT 
ATTTTTACAC CACAGCTTCA GCAAGAGTTC ATAGCACCAG CAGAGGATTC CAAGCGATCC 
GAGATTCAAG AAGAATTCTT ATCAGCACCA CCACAAGAAT CTAATCTATC AACCGAACTT 
AGAGAAGATA CACACACTGG TCCACCCGTA ATCGAACCCG TTTTGGAAGA AACCCCAGCA 
AGTAGCTCGG AATCGAGTGT TCCGCCCGCA AGTAAGGTAG ATCAAGAAGA TATAGTCTAC 
CCAGAACCAT CTGCGCCGGT GGAACAAGAG ATTCCATTAT TCGTTCCAGT GCCAGGTTTG 
CCGGGACTAA GTCAGGACAT ACCACTATAT CCACCACCAC CAACTGGTTC CACCACTGCC 
AGCTCGATTT TTGCTGCGCC ACCAGTGGCT TCAAGTGCTC CACTTTTTGT TCCTGTTCCT 
GGTCCTCCGT CGGTTTCATC GTTATTTTCC CCCTCTCAAG TGGATTCTAC TTCTTCGTTA 
TTTGCTCCGC CACCAACATC TGCAAATACA ACAATACTAA CACCGGCTCC GGATACGACT 
TCTACGTTGT TTGCCAACAA CATACCAACA ACAACACCAT CCATACCAGA TATAGTTCCA 
ACTACATCGG CTGAGCTGGT TGACGCACCT CCTGGTTTCC AGGGATCATC AGACGACACC 
GCAACCAATC TATTTCAGGG AAACACCGAA ACCCAAACAG TTTTTAGTCC ATTTGCACAA 
ACTAAGGCTA CACCTTTGGT TCAGGAAACA AAACCCGAAT TGGTTCCACC TCCAATTGGA 
TTCTTTGCGC CGAAAAGCGA GTCGGAAGTT CTTAGCCAGC CACCACTTGC TCCCTCGCCA 
CTGCAAAGCT TCTTCAGCAC AGCACCAGAA ACAGCAACAA CCACCGACTT CAATTTCTTC 
GGAAGCCCAG CACCCGGCAG CGTGTTTCCT GATTTGCCAG TGCAGCAGGG AATCGCACCG 
CCACCATTGG CACTTGAGTC ACCGCCACAG AATAGTGTGT CCAATATAGC CACATCTGCT 
ATAGGATTCG ATTTGTTAAA CCAGACTCAG CAAGCTTCAA GTTTAGTCGA AAACTCAAAT 
CAAAATTCAA GTGTCAACTC TTTCGACGGC TATTTTGGTG GTCCTAGTGC ATCTGCGGCA 
CAAACATTCG CAAACGAGAC AACTAATGCA CTTGTTGCTC CAGCTATAAA CTGCGATCCC 
GTCAATTTCT TCGATCAGGT TCCTCAAACC CAGTCGCAAA TCCAGCAAGC AAACGAGGAT 
CAGCGCATTC AGAACTTCTT CAACAATCCA CCGTTGCAGG ATCAACCCGC TGTACCAGGT 
GAACTCAAAT ACGACATTGT CCACAGCGGA GTGGCTGTTA AGCAGCTGCA AGAGCGCTCA 
CAGACGCCCG TTTCAAATCT TGTCGAGCCA CCTTCGTCTG CCTGTTCGGA ATTCTCGACC 
CTTGCTCCAG CACCAACACA AACCGATCGC CAATCGGGCG ACGATCTACT CCAACAGCAT 
CTGGGCGAAT TGCCCGAGGA TATCCTCAGA GAGCTTAGAA TGGCAAGTGC CAGCAGCGAA 
AAGGGCCAGG TGGCGACGCC TCCCGTGGCT ATACCTTACT CACCAGTGGT GGCCCATTGG 
TTCTACAAGC GCAGCGTGGA CACAAAGTTC ATCTGGACAC CATTCAGCCA CTATGATTCG 
GCCTTGTTAG AAACCAGCCT AAATCTTGAC GATTCCACAT TGATTATTCC CGTTGAGGGT 
GGCCGCTACG ATGTAAACAT CAAGGAACGT ACCAAGACTC CCGTTTACTG GGAGGGAAAG 
GCCATCGAAG TGCGACGCTG CTCCTGGTTC TACAAGGGAG TTGACTCCAA GTATGTTCCA 
TACACCGAGG ATACGGCTGC ACTACTTGAA GCGGAATACA AGCGATCTGC GGAGACTGGT 
GAATGGCATC AGAAGATTAT GCTGGCCAAT GGCGAACAGG TGGTCTTCCA CGGACCTACA 
GTCATCGTTC ACTTCCTGCC GCAACAGAAT GCAGACACTT GGGGAGCCAG CACACAGGGT 
TCCATGCGTC CGAGAGTTGT CAAGAGGGAT CTGGATGATT TCACCATCGA GCAAGGCGAA 
TCGCAGCGAG TCGATCACCT GCTTTTCATG GTCCATGGCA TTGGCTCAGC CTGCGATTTG 
AAAATGCGTT CCGTGGAGGA AGTGGTGGAT GACTTCCGGG TTATTGCCCA GCAACTAGTT 
CAGTCGCACT ACAAAAACTC AACAGATATG GGCCTGGTGG GTCGCGTTGA AGTTTTGCCC 
ATCTCGTGGC ACGGACACCT GCACTCCGAG GAGTTAGGCA TCGACGAAAA GCTGAAATCC 
ATCACTTTGG AGTCTATTCC TCGCTTGCGA AACTTCACCA ATGACACGCT GCTCGACGTG 
CTGTTCTACA CCAGTCCGAA ATACTGTCAA AAGATCATGA ACACGGTGGC AGATGCGCTT 
AACGATGTCT ACCTGAAGTA CCGAATGCGG CACCCTGAAT TCAATGGGGG AGTTTCGCTG 
GCAGGTCACA GTCTCGGCTC CCTAATCCTA TTCGATTTAT TGTGCCATCA GGAACCGCTC 
AAGGAAAGCG AGGAAGAGAA TAAAGAGAAT CCCGACCAAC TTCCCCAAAA GCAATCGAGT 
CAAAAGGTAC AGCTTCCAAC CAGCGATCCT TTGCCGAAAC AAGTCAGCTA TGCGATGGGT 
CCGGAGGGCA CGGGACAGCC CGTGATTACA TATACACAGC TGATATTCCA CCCCAAAAAA 
TTCTTTGCTC TGGGCTCACC CATCGGAATG TTTGTAACCA TCAGGGGGAT TGATAAGCTT 
GGTCTCGACT TTCACCTGCC CACGTGTCCG GGTTTCTACA ACATATTCCA TCCCTTCGAT 
CCGGTAGCCT ATCGGATCGA GGCGCTTGTC AATCCGGACA TGAACGGCAT TCGACCGGTG 
TTAATTCCCC ATCACAAGGG ACGAAAACGA ATGCACCTGG AGCTGAAGGA GACGATGACA 
CGTGTGGGTG CGGACATCAA GCAGCGATTT ATGGACACAT TCAAGACCAC GCTGGACAGC 
GTAAACTTTT TGACCACGGT AACGAAGGTT AAGAAGGAAG CGGAGGAGTC GCTGGAGAAG 
GAGACAAGCC AGACTTCATC GCAAGCTTTT CAAAAGCAAA CCGAGGATCA GGACGAGAGT 
TCAGTTGCCA GTTCATCTTG CAAACTACGA AATCGCACCG ATTCCAATTC GACGACGGCT 
TCTGACCCTG AGTTTATAGA ACTTGATTTC CCACTGGGTA AGCTGAACGA CTCAAAACGT 
GTGGACTACG TGCTTCAGGA AGCTCCACTA GAATTCATCA ACGAATATAT TTTCGCCCTG 
AGCAGCCATG TGTGCTACTG GGGCTCCGAA GATACGATCC TTTTCGTGAT GAAGGAGATT 
TATGCCAGCC TGGGAATCAG CACAGACAGC CAGGTGCCGC AGTCCTCAAT GACCATCGAG 
CGACCCGTTT CGCATGAGAG CAGTGTTAGC CACTCGCTGT TGCCGATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609185.1
CDS	144..6194
Translation	MSERSKGDVSARVNNNANQTRVVKNPLLSASVTGLSFDDFPNKPQLLPAAPPVVHATPPAAPVLIAGILNREPKSLVTVGQPSSIDDSEALDEINLNTSSEDSVGAGGSSPYLGAGDQNANPLLEPPTEAGNDSLLSQAASSFTALPAVASNVFSTFSKRIYAGSRETSEEPKLDIQPSYIQQASYVQPVAPVPAPFYAAPPAAANEVVAPEPPKFYAPTEVPPPNVGVPAPPPTSGNPNTYRFTARKKLYAPIPGLSEQSGQPAQQPQVPQAPQAAPSFQTPPYPPQLTYAANPAPFDISAQPAAIPAEERKSGGGLFSLTSLVPTGVLQNITGIVQSATGRSSEPEHTNVQQAGGYLDITTASAPAPSTNLFGGPNSTFPPGGNYFVPGAPPSLPSTGVVGPPSVGNFFAPAAPLPVAQTAAPPAVGAFFTSAAPSVTPSVFTPGTVPPLAPQPFQSATVNQSALPPNNPPTASAVGELFVPAPTTIAQGTSPAVPFVDTQAVPTIPLTVPPGNPPPTSTLGGFFTTGEVAPVVAPFGTQASPATQSAGFFNPGEVRPNVPLAEPVPTIPITSSGVPPIGLFPPAPAQPGVQTASNPNPLPGAAAPPPAAGQASYRLQKGTRLYKSPLTPQETATSSGFAAPLPPTSVTPAIFNPFGAPAPGSFNSVGHLEGQSSQGVPFFTPPVQNPPGQVAAPTVAVPQSAPIAPPPGTSSTSVAPPLTASIANPPPTSGFASVPLFAAPTISSTPIPFFNPQPTGAEVTTGLSQQENEATASTTLEGLVQGPAPTLSASQFFDTPLPTGTQPENQVASVSSERENLVQEPISNQGIPFFAPPQTNIFTPQLQQEFIAPAEDSKRSEIQEEFLSAPPQESNLSTELREDTHTGPPVIEPVLEETPASSSESSVPPASKVDQEDIVYPEPSAPVEQEIPLFVPVPGLPGLSQDIPLYPPPPTGSTTASSIFAAPPVASSAPLFVPVPGPPSVSSLFSPSQVDSTSSLFAPPPTSANTTILTPAPDTTSTLFANNIPTTTPSIPDIVPTTSAELVDAPPGFQGSSDDTATNLFQGNTETQTVFSPFAQTKATPLVQETKPELVPPPIGFFAPKSESEVLSQPPLAPSPLQSFFSTAPETATTTDFNFFGSPAPGSVFPDLPVQQGIAPPPLALESPPQNSVSNIATSAIGFDLLNQTQQASSLVENSNQNSSVNSFDGYFGGPSASAAQTFANETTNALVAPAINCDPVNFFDQVPQTQSQIQQANEDQRIQNFFNNPPLQDQPAVPGELKYDIVHSGVAVKQLQERSQTPVSNLVEPPSSACSEFSTLAPAPTQTDRQSGDDLLQQHLGELPEDILRELRMASASSEKGQVATPPVAIPYSPVVAHWFYKRSVDTKFIWTPFSHYDSALLETSLNLDDSTLIIPVEGGRYDVNIKERTKTPVYWEGKAIEVRRCSWFYKGVDSKYVPYTEDTAALLEAEYKRSAETGEWHQKIMLANGEQVVFHGPTVIVHFLPQQNADTWGASTQGSMRPRVVKRDLDDFTIEQGESQRVDHLLFMVHGIGSACDLKMRSVEEVVDDFRVIAQQLVQSHYKNSTDMGLVGRVEVLPISWHGHLHSEELGIDEKLKSITLESIPRLRNFTNDTLLDVLFYTSPKYCQKIMNTVADALNDVYLKYRMRHPEFNGGVSLAGHSLGSLILFDLLCHQEPLKESEEENKENPDQLPQKQSSQKVQLPTSDPLPKQVSYAMGPEGTGQPVITYTQLIFHPKKFFALGSPIGMFVTIRGIDKLGLDFHLPTCPGFYNIFHPFDPVAYRIEALVNPDMNGIRPVLIPHHKGRKRMHLELKETMTRVGADIKQRFMDTFKTTLDSVNFLTTVTKVKKEAEESLEKETSQTSSQAFQKQTEDQDESSVASSSCKLRNRTDSNSTTASDPEFIELDFPLGKLNDSKRVDYVLQEAPLEFINEYIFALSSHVCYWGSEDTILFVMKEIYASLGISTDSQVPQSSMTIERPVSHESSVSHSLLPM

Target ORF information:

RefSeq Version	NM_135341.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatidic acid phospholipase A1, transcript variant A (PAPLA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135341.1

ORF Insert Sequence:

ATGTCGGAAA GATCGAAAGG TGACGTGTCC GCTCGCGTAA ACAACAACGC AAACCAGACG 
CGTGTCGTTA AAAATCCGCT TCTGAGCGCC TCCGTGACCG GCCTGAGTTT CGACGACTTC 
CCCAACAAGC CGCAGCTCCT GCCAGCCGCC CCGCCCGTGG TCCATGCCAC GCCTCCAGCG 
GCGCCCGTCC TCATCGCTGG CATCTTGAAT CGCGAACCGA AATCATTGGT CACCGTTGGT 
CAGCCATCGA GCATTGACGA TAGCGAGGCG CTCGACGAGA TTAACTTGAA TACCAGCAGC 
GAGGATTCCG TTGGCGCAGG TGGATCGAGT CCATATCTAG GAGCAGGCGA TCAAAACGCC 
AATCCTCTGT TGGAACCACC AACGGAAGCT GGAAACGATT CGTTGCTGAG CCAGGCGGCC 
TCCTCCTTTA CTGCCCTGCC TGCGGTGGCA TCCAATGTGT TTTCCACCTT CTCGAAGCGG 
ATCTACGCCG GCAGCAGGGA GACCTCGGAG GAGCCCAAAC TAGACATACA GCCCTCTTAC 
ATTCAGCAGG CCAGCTACGT TCAGCCAGTA GCTCCAGTGC CAGCTCCCTT TTATGCAGCA 
CCGCCAGCGG CCGCAAACGA AGTGGTCGCC CCCGAACCAC CCAAGTTCTA CGCACCTACT 
GAGGTTCCGC CACCGAATGT AGGAGTCCCT GCACCACCGC CTACATCGGG AAATCCAAAC 
ACATACAGAT TCACCGCCAG GAAGAAGCTA TACGCTCCCA TTCCTGGATT ATCCGAGCAG 
TCCGGACAAC CTGCACAACA GCCACAGGTT CCTCAGGCTC CCCAAGCGGC TCCATCATTC 
CAGACGCCAC CATATCCACC ACAGCTAACG TACGCAGCAA ACCCTGCTCC CTTTGACATA 
TCAGCGCAGC CAGCTGCAAT TCCCGCAGAG GAACGCAAAT CTGGTGGAGG ACTCTTCTCT 
TTGACCAGTC TCGTGCCCAC AGGAGTGCTT CAAAATATCA CAGGAATTGT TCAATCAGCC 
ACTGGGCGAA GCAGTGAACC AGAACACACT AACGTCCAGC AAGCAGGAGG CTACCTAGAC 
ATTACAACAG CATCAGCACC AGCTCCATCG ACCAACTTAT TTGGTGGACC GAATTCAACC 
TTTCCGCCGG GAGGAAATTA TTTCGTACCG GGAGCACCTC CATCATTACC CTCAACAGGG 
GTAGTAGGCC CCCCTTCGGT CGGAAACTTC TTCGCGCCGG CTGCTCCATT GCCAGTAGCA 
CAAACAGCTG CTCCACCAGC TGTCGGAGCC TTCTTCACAT CTGCTGCCCC AAGTGTTACG 
CCTTCAGTTT TTACTCCAGG AACAGTTCCA CCTCTTGCAC CGCAGCCTTT TCAGTCAGCA 
ACTGTAAACC AATCTGCATT GCCACCAAAC AATCCGCCGA CCGCATCTGC AGTAGGAGAA 
CTCTTTGTTC CAGCACCAAC GACAATCGCC CAAGGAACTT CACCTGCAGT TCCATTTGTT 
GACACACAAG CTGTTCCAAC AATACCTTTA ACAGTTCCAC CAGGCAATCC TCCTCCAACA 
TCGACACTTG GAGGATTCTT TACCACAGGT GAAGTTGCAC CTGTTGTTGC GCCATTTGGA 
ACACAAGCGT CACCAGCAAC ACAATCTGCA GGATTCTTTA ATCCAGGAGA AGTTCGTCCT 
AATGTCCCGT TAGCCGAACC TGTTCCAACT ATACCGATTA CATCATCAGG TGTTCCACCT 
ATAGGATTAT TTCCTCCAGC ACCGGCACAG CCTGGTGTCC AAACTGCTTC AAATCCGAAT 
CCCTTGCCAG GAGCAGCTGC ACCGCCACCA GCCGCTGGGC AAGCTTCATA TCGTCTCCAG 
AAGGGAACTC GTCTTTACAA GAGTCCATTG ACGCCCCAGG AAACCGCAAC ATCTTCGGGA 
TTCGCCGCTC CTCTGCCCCC CACATCTGTA ACTCCAGCAA TTTTTAATCC ATTCGGAGCA 
CCGGCACCAG GATCTTTCAA TTCAGTAGGC CATCTAGAGG GTCAATCCAG CCAAGGAGTT 
CCTTTCTTTA CGCCGCCAGT GCAGAATCCA CCAGGACAAG TAGCGGCTCC AACTGTTGCT 
GTTCCGCAAT CAGCACCTAT TGCCCCTCCA CCAGGGACAT CAAGCACAAG TGTTGCTCCT 
CCGCTAACAG CATCTATAGC CAATCCTCCT CCAACATCTG GCTTTGCAAG TGTACCCCTC 
TTCGCTGCAC CAACAATATC CTCAACGCCT ATTCCTTTCT TCAATCCTCA ACCAACAGGA 
GCAGAAGTAA CAACTGGTTT GAGCCAACAG GAAAACGAAG CTACAGCATC GACAACTTTG 
GAAGGTCTAG TGCAAGGACC TGCTCCAACA CTCTCTGCAA GTCAATTCTT TGATACGCCC 
TTGCCAACAG GAACCCAACC GGAGAATCAA GTAGCAAGTG TTTCCTCGGA AAGAGAAAAT 
CTTGTACAGG AGCCAATATC GAACCAAGGA ATTCCATTCT TTGCGCCGCC GCAAACGAAT 
ATTTTTACAC CACAGCTTCA GCAAGAGTTC ATAGCACCAG CAGAGGATTC CAAGCGATCC 
GAGATTCAAG AAGAATTCTT ATCAGCACCA CCACAAGAAT CTAATCTATC AACCGAACTT 
AGAGAAGATA CACACACTGG TCCACCCGTA ATCGAACCCG TTTTGGAAGA AACCCCAGCA 
AGTAGCTCGG AATCGAGTGT TCCGCCCGCA AGTAAGGTAG ATCAAGAAGA TATAGTCTAC 
CCAGAACCAT CTGCGCCGGT GGAACAAGAG ATTCCATTAT TCGTTCCAGT GCCAGGTTTG 
CCGGGACTAA GTCAGGACAT ACCACTATAT CCACCACCAC CAACTGGTTC CACCACTGCC 
AGCTCGATTT TTGCTGCGCC ACCAGTGGCT TCAAGTGCTC CACTTTTTGT TCCTGTTCCT 
GGTCCTCCGT CGGTTTCATC GTTATTTTCC CCCTCTCAAG TGGATTCTAC TTCTTCGTTA 
TTTGCTCCGC CACCAACATC TGCAAATACA ACAATACTAA CACCGGCTCC GGATACGACT 
TCTACGTTGT TTGCCAACAA CATACCAACA ACAACACCAT CCATACCAGA TATAGTTCCA 
ACTACATCGG CTGAGCTGGT TGACGCACCT CCTGGTTTCC AGGGATCATC AGACGACACC 
GCAACCAATC TATTTCAGGG AAACACCGAA ACCCAAACAG TTTTTAGTCC ATTTGCACAA 
ACTAAGGCTA CACCTTTGGT TCAGGAAACA AAACCCGAAT TGGTTCCACC TCCAATTGGA 
TTCTTTGCGC CGAAAAGCGA GTCGGAAGTT CTTAGCCAGC CACCACTTGC TCCCTCGCCA 
CTGCAAAGCT TCTTCAGCAC AGCACCAGAA ACAGCAACAA CCACCGACTT CAATTTCTTC 
GGAAGCCCAG CACCCGGCAG CGTGTTTCCT GATTTGCCAG TGCAGCAGGG AATCGCACCG 
CCACCATTGG CACTTGAGTC ACCGCCACAG AATAGTGTGT CCAATATAGC CACATCTGCT 
ATAGGATTCG ATTTGTTAAA CCAGACTCAG CAAGCTTCAA GTTTAGTCGA AAACTCAAAT 
CAAAATTCAA GTGTCAACTC TTTCGACGGC TATTTTGGTG GTCCTAGTGC ATCTGCGGCA 
CAAACATTCG CAAACGAGAC AACTAATGCA CTTGTTGCTC CAGCTATAAA CTGCGATCCC 
GTCAATTTCT TCGATCAGGT TCCTCAAACC CAGTCGCAAA TCCAGCAAGC AAACGAGGAT 
CAGCGCATTC AGAACTTCTT CAACAATCCA CCGTTGCAGG ATCAACCCGC TGTACCAGGT 
GAACTCAAAT ACGACATTGT CCACAGCGGA GTGGCTGTTA AGCAGCTGCA AGAGCGCTCA 
CAGACGCCCG TTTCAAATCT TGTCGAGCCA CCTTCGTCTG CCTGTTCGGA ATTCTCGACC 
CTTGCTCCAG CACCAACACA AACCGATCGC CAATCGGGCG ACGATCTACT CCAACAGCAT 
CTGGGCGAAT TGCCCGAGGA TATCCTCAGA GAGCTTAGAA TGGCAAGTGC CAGCAGCGAA 
AAGGGCCAGG TGGCGACGCC TCCCGTGGCT ATACCTTACT CACCAGTGGT GGCCCATTGG 
TTCTACAAGC GCAGCGTGGA CACAAAGTTC ATCTGGACAC CATTCAGCCA CTATGATTCG 
GCCTTGTTAG AAACCAGCCT AAATCTTGAC GATTCCACAT TGATTATTCC CGTTGAGGGT 
GGCCGCTACG ATGTAAACAT CAAGGAACGT ACCAAGACTC CCGTTTACTG GGAGGGAAAG 
GCCATCGAAG TGCGACGCTG CTCCTGGTTC TACAAGGGAG TTGACTCCAA GTATGTTCCA 
TACACCGAGG ATACGGCTGC ACTACTTGAA GCGGAATACA AGCGATCTGC GGAGACTGGT 
GAATGGCATC AGAAGATTAT GCTGGCCAAT GGCGAACAGG TGGTCTTCCA CGGACCTACA 
GTCATCGTTC ACTTCCTGCC GCAACAGAAT GCAGACACTT GGGGAGCCAG CACACAGGGT 
TCCATGCGTC CGAGAGTTGT CAAGAGGGAT CTGGATGATT TCACCATCGA GCAAGGCGAA 
TCGCAGCGAG TCGATCACCT GCTTTTCATG GTCCATGGCA TTGGCTCAGC CTGCGATTTG 
AAAATGCGTT CCGTGGAGGA AGTGGTGGAT GACTTCCGGG TTATTGCCCA GCAACTAGTT 
CAGTCGCACT ACAAAAACTC AACAGATATG GGCCTGGTGG GTCGCGTTGA AGTTTTGCCC 
ATCTCGTGGC ACGGACACCT GCACTCCGAG GAGTTAGGCA TCGACGAAAA GCTGAAATCC 
ATCACTTTGG AGTCTATTCC TCGCTTGCGA AACTTCACCA ATGACACGCT GCTCGACGTG 
CTGTTCTACA CCAGTCCGAA ATACTGTCAA AAGATCATGA ACACGGTGGC AGATGCGCTT 
AACGATGTCT ACCTGAAGTA CCGAATGCGG CACCCTGAAT TCAATGGGGG AGTTTCGCTG 
GCAGGTCACA GTCTCGGCTC CCTAATCCTA TTCGATTTAT TGTGCCATCA GGAACCGCTC 
AAGGAAAGCG AGGAAGAGAA TAAAGAGAAT CCCGACCAAC TTCCCCAAAA GCAATCGAGT 
CAAAAGGTAC AGCTTCCAAC CAGCGATCCT TTGCCGAAAC AAGTCAGCTA TGCGATGGGT 
CCGGAGGGCA CGGGACAGCC CGTGATTACA TATACACAGC TGATATTCCA CCCCAAAAAA 
TTCTTTGCTC TGGGCTCACC CATCGGAATG TTTGTAACCA TCAGGGGGAT TGATAAGCTT 
GGTCTCGACT TTCACCTGCC CACGTGTCCG GGTTTCTACA ACATATTCCA TCCCTTCGAT 
CCGGTAGCCT ATCGGATCGA GGCGCTTGTC AATCCGGACA TGAACGGCAT TCGACCGGTG 
TTAATTCCCC ATCACAAGGG ACGAAAACGA ATGCACCTGG AGCTGAAGGA GACGATGACA 
CGTGTGGGTG CGGACATCAA GCAGCGATTT ATGGACACAT TCAAGACCAC GCTGGACAGC 
GTAAACTTTT TGACCACGGT AACGAAGGTT AAGAAGGAAG CGGAGGAGTC GCTGGAGAAG 
GAGACAAGCC AGACTTCATC GCAAGCTTTT CAAAAGCAAA CCGAGGATCA GGACGAGAGT 
TCAGTTGCCA GTTCATCTTG CAAACTACGA AATCGCACCG ATTCCAATTC GACGACGGCT 
TCTGACCCTG AGTTTATAGA ACTTGATTTC CCACTGGGTA AGCTGAACGA CTCAAAACGT 
GTGGACTACG TGCTTCAGGA AGCTCCACTA GAATTCATCA ACGAATATAT TTTCGCCCTG 
AGCAGCCATG TGTGCTACTG GGGCTCCGAA GATACGATCC TTTTCGTGAT GAAGGAGATT 
TATGCCAGCC TGGGAATCAG CACAGACAGC CAGGTGCCGC AGTCCTCAAT GACCATCGAG 
CGACCCGTTT CGCATGAGAG CAGTGTTAGC CACTCGCTGT TGCCGATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPLA1 ( NM_001298812.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285741.1 Drosophila melanogaster phosphatidic acid phospholipase A1, transcript variant D (PAPLA1), mRNA.

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CloneID	OFa21617
Clone ID Related Accession (Same CDS sequence)	NM_001298812.1
Accession Version	NM_001298812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatidic acid phospholipase A1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGAAA GATCGAAAGG TGACGTGTCC GCTCGCGTAA ACAACAACGC AAACCAGACG 
CGTGTCGTTA AAAATCCGCT TCTGAGCGCC TCCGTGACCG GCCTGAGTTT CGACGACTTC 
CCCAACAAGC CGCAGCTCCT GCCAGCCGCC CCGCCCGTGG TCCATGCCAC GCCTCCAGCG 
GCGCCCGTCC TCATCGCTGG CATCTTGAAT CGCGAACCGA AATCATTGGT CACCGTTGGT 
CAGCCATCGA GCATTGACGA TAGCGAGGCG CTCGACGAGA TTAACTTGAA TACCAGCAGC 
GAGGATTCCG TTGGCGCAGG TGGATCGAGT CCATATCTAG GAGCAGGCGA TCAAAACGCC 
AATCCTCTGT TGGAACCACC AACGGAAGCT GGAAACGATT CGTTGCTGAG CCAGGCGGCC 
TCCTCCTTTA CTGCCCTGCC TGCGGTGGCA TCCAATGTGT TTTCCACCTT CTCGAAGCGG 
ATCTACGCCG GCAGCAGGGA GACCTCGGAG GAGCCCAAAC TAGACATACA GCCCTCTTAC 
ATTCAGCAGG CCAGCTACGT TCAGCCAGTA GCTCCAGTGC CAGCTCCCTT TTATGCAGCA 
CCGCCAGCGG CCGCAAACGA AGTGGTCGCC CCCGAACCAC CCAAGTTCTA CGCACCTACT 
GAGGTTCCGC CACCGAATGT AGGAGTCCCT GCACCACCGC CTACATCGGG AAATCCAAAC 
ACATACAGAT TCACCGCCAG GAAGAAGCTA TACGCTCCCA TTCCTGGATT ATCCGAGCAG 
TCCGGACAAC CTGCACAACA GCCACAGGTT CCTCAGGCTC CCCAAGCGGC TCCATCATTC 
CAGACGCCAC CATATCCACC ACAGCTAACG TACGCAGCAA ACCCTGCTCC CTTTGACATA 
TCAGCGCAGC CAGCTGCAAT TCCCGCAGAG GAACGCAAAT CTGGTGGAGG ACTCTTCTCT 
TTGACCAGTC TCGTGCCCAC AGGAGTGCTT CAAAATATCA CAGGAATTGT TCAATCAGCC 
ACTGGGCGAA GCAGTGAACC AGAACACACT AACGTCCAGC AAGCAGGAGG CTACCTAGAC 
ATTACAACAG CATCAGCACC AGCTCCATCG ACCAACTTAT TTGGTGGACC GAATTCAACC 
TTTCCGCCGG GAGGAAATTA TTTCGTACCG GGAGCACCTC CATCATTACC CTCAACAGGG 
GTAGTAGGCC CCCCTTCGGT CGGAAACTTC TTCGCGCCGG CTGCTCCATT GCCAGTAGCA 
CAAACAGCTG CTCCACCAGC TGTCGGAGCC TTCTTCACAT CTGCTGCCCC AAGTGTTACG 
CCTTCAGTTT TTACTCCAGG AACAGTTCCA CCTCTTGCAC CGCAGCCTTT TCAGTCAGCA 
ACTGTAAACC AATCTGCATT GCCACCAAAC AATCCGCCGA CCGCATCTGC AGTAGGAGAA 
CTCTTTGTTC CAGCACCAAC GACAATCGCC CAAGGAACTT CACCTGCAGT TCCATTTGTT 
GACACACAAG CTGTTCCAAC AATACCTTTA ACAGTTCCAC CAGGCAATCC TCCTCCAACA 
TCGACACTTG GAGGATTCTT TACCACAGGT GAAGTTGCAC CTGTTGTTGC GCCATTTGGA 
ACACAAGCGT CACCAGCAAC ACAATCTGCA GGATTCTTTA ATCCAGGAGA AGTTCGTCCT 
AATGTCCCGT TAGCCGAACC TGTTCCAACT ATACCGATTA CATCATCAGG TGTTCCACCT 
ATAGGATTAT TTCCTCCAGC ACCGGCACAG CCTGGTGTCC AAACTGCTTC AAATCCGAAT 
CCCTTGCCAG GAGCAGCTGC ACCGCCACCA GCCGCTGGGC AAGCTTCATA TCGTCTCCAG 
AAGGGAACTC GTCTTTACAA GAGTCCATTG ACGCCCCAGG AAACCGCAAC ATCTTCGGGA 
TTCGCCGCTC CTCTGCCCCC CACATCTGTA ACTCCAGCAA TTTTTAATCC ATTCGGAGCA 
CCGGCACCAG GATCTTTCAA TTCAGTAGGC CATCTAGAGG GTCAATCCAG CCAAGGAGTT 
CCTTTCTTTA CGCCGCCAGT GCAGAATCCA CCAGGACAAG TAGCGGCTCC AACTGTTGCT 
GTTCCGCAAT CAGCACCTAT TGCCCCTCCA CCAGGGACAT CAAGCACAAG TGTTGCTCCT 
CCGCTAACAG CATCTATAGC CAATCCTCCT CCAACATCTG GCTTTGCAAG TGTACCCCTC 
TTCGCTGCAC CAACAATATC CTCAACGCCT ATTCCTTTCT TCAATCCTCA ACCAACAGGA 
GCAGAAGTAA CAACTGGTTT GAGCCAACAG GAAAACGAAG CTACAGCATC GACAACTTTG 
GAAGGTCTAG TGCAAGGACC TGCTCCAACA CTCTCTGCAA GTCAATTCTT TGATACGCCC 
TTGCCAACAG GAACCCAACC GGAGAATCAA GTAGCAAGTG TTTCCTCGGA AAGAGAAAAT 
CTTGTACAGG AGCCAATATC GAACCAAGGA ATTCCATTCT TTGCGCCGCC GCAAACGAAT 
ATTTTTACAC CACAGCTTCA GCAAGAGTTC ATAGCACCAG CAGAGGATTC CAAGCGATCC 
GAGATTCAAG AAGAATTCTT ATCAGCACCA CCACAAGAAT CTAATCTATC AACCGAACTT 
AGAGAAGATA CACACACTGG TCCACCCGTA ATCGAACCCG TTTTGGAAGA AACCCCAGCA 
AGTAGCTCGG AATCGAGTGT TCCGCCCGCA AGTAAGGTAG ATCAAGAAGA TATAGTCTAC 
CCAGAACCAT CTGCGCCGGT GGAACAAGAG ATTCCATTAT TCGTTCCAGT GCCAGGTTTG 
CCGGGACTAA GTCAGGACAT ACCACTATAT CCACCACCAC CAACTGGTTC CACCACTGCC 
AGCTCGATTT TTGCTGCGCC ACCAGTGGCT TCAAGTGCTC CACTTTTTGT TCCTGTTCCT 
GGTCCTCCGT CGGTTTCATC GTTATTTTCC CCCTCTCAAG TGGATTCTAC TTCTTCGTTA 
TTTGCTCCGC CACCAACATC TGCAAATACA ACAATACTAA CACCGGCTCC GGATACGACT 
TCTACGTTGT TTGCCAACAA CATACCAACA ACAACACCAT CCATACCAGA TATAGTTCCA 
ACTACATCGG CTGAGCTGGT TGACGCACCT CCTGGTTTCC AGGGATCATC AGACGACACC 
GCAACCAATC TATTTCAGGG AAACACCGAA ACCCAAACAG TTTTTAGTCC ATTTGCACAA 
ACTAAGGCTA CACCTTTGGT TCAGGAAACA AAACCCGAAT TGGTTCCACC TCCAATTGGA 
TTCTTTGCGC CGAAAAGCGA GTCGGAAGTT CTTAGCCAGC CACCACTTGC TCCCTCGCCA 
CTGCAAAGCT TCTTCAGCAC AGCACCAGAA ACAGCAACAA CCACCGACTT CAATTTCTTC 
GGAAGCCCAG CACCCGGCAG CGTGTTTCCT GATTTGCCAG TGCAGCAGGG AATCGCACCG 
CCACCATTGG CACTTGAGTC ACCGCCACAG AATAGTGTGT CCAATATAGC CACATCTGCT 
ATAGGATTCG ATTTGTTAAA CCAGACTCAG CAAGCTTCAA GTTTAGTCGA AAACTCAAAT 
CAAAATTCAA GTGTCAACTC TTTCGACGGC TATTTTGGTG GTCCTAGTGC ATCTGCGGCA 
CAAACATTCG CAAACGAGAC AACTAATGCA CTTGTTGCTC CAGCTATAAA CTGCGATCCC 
GTCAATTTCT TCGATCAGGT TCCTCAAACC CAGTCGCAAA TCCAGCAAGC AAACGAGGAT 
CAGCGCATTC AGAACTTCTT CAACAATCCA CCGTTGCAGG ATCAACCCGC TGTACCAGGT 
GAACTCAAAT ACGACATTGT CCACAGCGGA GTGGCTGTTA AGCAGCTGCA AGAGCGCTCA 
CAGACGCCCG TTTCAAATCT TGTCGAGCCA CCTTCGTCTG CCTGTTCGGA ATTCTCGACC 
CTTGCTCCAG CACCAACACA AACCGATCGC CAATCGGGCG ACGATCTACT CCAACAGCAT 
CTGGGCGAAT TGCCCGAGGA TATCCTCAGA GAGCTTAGAA TGGCAAGTGC CAGCAGCGAA 
AAGGGCCAGG TGGCGACGCC TCCCGTGGCT ATACCTTACT CACCAGTGGT GGCCCATTGG 
TTCTACAAGC GCAGCGTGGA CACAAAGTTC ATCTGGACAC CATTCAGCCA CTATGATTCG 
GCCTTGTTAG AAACCAGCCT AAATCTTGAC GATTCCACAT TGATTATTCC CGTTGAGGGT 
GGCCGCTACG ATGTAAACAT CAAGGAACGT ACCAAGACTC CCGTTTACTG GGAGGGAAAG 
GCCATCGAAG TGCGACGCTG CTCCTGGTTC TACAAGGGAG TTGACTCCAA GTATGTTCCA 
TACACCGAGG ATACGGCTGC ACTACTTGAA GCGGAATACA AGCGATCTGC GGAGACTGGT 
GAATGGCATC AGAAGATTAT GCTGGCCAAT GGCGAACAGG TGGTCTTCCA CGGACCTACA 
GTCATCGTTC ACTTCCTGCC GCAACAGAAT GCAGACACTT GGGGAGCCAG CACACAGGGT 
TCCATGCGTC CGAGAGTTGT CAAGAGGGAT CTGGATGATT TCACCATCGA GCAAGGCGAA 
TCGCAGCGAG TCGATCACCT GCTTTTCATG GTCCATGGCA TTGGCTCAGC CTGCGATTTG 
AAAATGCGTT CCGTGGAGGA AGTGGTGGAT GACTTCCGGG TTATTGCCCA GCAACTAGTT 
CAGTCGCACT ACAAAAACTC AACAGATATG GGCCTGGTGG GTCGCGTTGA AGTTTTGCCC 
ATCTCGTGGC ACGGACACCT GCACTCCGAG GAGTTAGGCA TCGACGAAAA GCTGAAATCC 
ATCACTTTGG AGTCTATTCC TCGCTTGCGA AACTTCACCA ATGACACGCT GCTCGACGTG 
CTGTTCTACA CCAGTCCGAA ATACTGTCAA AAGATCATGA ACACGGTGGC AGATGCGCTT 
AACGATGTCT ACCTGAAGTA CCGAATGCGG CACCCTGAAT TCAATGGGGG AGTTTCGCTG 
GCAGGTCACA GTCTCGGCTC CCTAATCCTA TTCGATTTAT TGTGCCATCA GGAACCGCTC 
AAGGAAAGCG AGGAAGAGAA TAAAAATCCC GACCAACTTC CCCAAAAGCA ATCGAGTCAA 
AAGGTACAGC TTCCAACCAG CGATCCTTTG CCGAAACAAG TCAGCTATGC GATGGGTCCG 
GAGGGCACGG GACAGCCCGT GATTACATAT ACACAGCTGA TATTCCACCC CAAAAAATTC 
TTTGCTCTGG GCTCACCCAT CGGAATGTTT GTAACCATCA GGGGGATTGA TAAGCTTGGT 
CTCGACTTTC ACCTGCCCAC GTGTCCGGGT TTCTACAACA TATTCCATCC CTTCGATCCG 
GTAGCCTATC GGATCGAGGC GCTTGTCAAT CCGGACATGA ACGGCATTCG ACCGGTGTTA 
ATTCCCCATC ACAAGGGACG AAAACGAATG CACCTGGAGC TGAAGGAGAC GATGACACGT 
GTGGGTGCGG ACATCAAGCA GCGATTTATG GACACATTCA AGACCACGCT GGACAGCGTA 
AACTTTTTGA CCACGGTAAC GAAGGTTAAG AAGGAAGCGG AGGAGTCGCT GGAGAAGGAG 
ACAAGCCAGA CTTCATCGCA AGCTTTTCAA AAGCAAACCG AGGATCAGGA CGAGAGTTCA 
GTTGCCAGTT CATCTTGCAA ACTACGAAAT CGCACCGATT CCAATTCGAC GACGGCTTCT 
GACCCTGAGT TTATAGAACT TGATTTCCCA CTGGGTAAGC TGAACGACTC AAAACGTGTG 
GACTACGTGC TTCAGGAAGC TCCACTAGAA TTCATCAACG AATATATTTT CGCCCTGAGC 
AGCCATGTGT GCTACTGGGG CTCCGAAGAT ACGATCCTTT TCGTGATGAA GGAGATTTAT 
GCCAGCCTGG GAATCAGCAC AGACAGCCAG GTGCCGCAGT CCTCAATGAC CATCGAGCGA 
CCCGTTTCGC ATGAGAGCAG TGTTAGCCAC TCGCTGTTGC CGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285741.1
CDS	207..6254
Translation	MSERSKGDVSARVNNNANQTRVVKNPLLSASVTGLSFDDFPNKPQLLPAAPPVVHATPPAAPVLIAGILNREPKSLVTVGQPSSIDDSEALDEINLNTSSEDSVGAGGSSPYLGAGDQNANPLLEPPTEAGNDSLLSQAASSFTALPAVASNVFSTFSKRIYAGSRETSEEPKLDIQPSYIQQASYVQPVAPVPAPFYAAPPAAANEVVAPEPPKFYAPTEVPPPNVGVPAPPPTSGNPNTYRFTARKKLYAPIPGLSEQSGQPAQQPQVPQAPQAAPSFQTPPYPPQLTYAANPAPFDISAQPAAIPAEERKSGGGLFSLTSLVPTGVLQNITGIVQSATGRSSEPEHTNVQQAGGYLDITTASAPAPSTNLFGGPNSTFPPGGNYFVPGAPPSLPSTGVVGPPSVGNFFAPAAPLPVAQTAAPPAVGAFFTSAAPSVTPSVFTPGTVPPLAPQPFQSATVNQSALPPNNPPTASAVGELFVPAPTTIAQGTSPAVPFVDTQAVPTIPLTVPPGNPPPTSTLGGFFTTGEVAPVVAPFGTQASPATQSAGFFNPGEVRPNVPLAEPVPTIPITSSGVPPIGLFPPAPAQPGVQTASNPNPLPGAAAPPPAAGQASYRLQKGTRLYKSPLTPQETATSSGFAAPLPPTSVTPAIFNPFGAPAPGSFNSVGHLEGQSSQGVPFFTPPVQNPPGQVAAPTVAVPQSAPIAPPPGTSSTSVAPPLTASIANPPPTSGFASVPLFAAPTISSTPIPFFNPQPTGAEVTTGLSQQENEATASTTLEGLVQGPAPTLSASQFFDTPLPTGTQPENQVASVSSERENLVQEPISNQGIPFFAPPQTNIFTPQLQQEFIAPAEDSKRSEIQEEFLSAPPQESNLSTELREDTHTGPPVIEPVLEETPASSSESSVPPASKVDQEDIVYPEPSAPVEQEIPLFVPVPGLPGLSQDIPLYPPPPTGSTTASSIFAAPPVASSAPLFVPVPGPPSVSSLFSPSQVDSTSSLFAPPPTSANTTILTPAPDTTSTLFANNIPTTTPSIPDIVPTTSAELVDAPPGFQGSSDDTATNLFQGNTETQTVFSPFAQTKATPLVQETKPELVPPPIGFFAPKSESEVLSQPPLAPSPLQSFFSTAPETATTTDFNFFGSPAPGSVFPDLPVQQGIAPPPLALESPPQNSVSNIATSAIGFDLLNQTQQASSLVENSNQNSSVNSFDGYFGGPSASAAQTFANETTNALVAPAINCDPVNFFDQVPQTQSQIQQANEDQRIQNFFNNPPLQDQPAVPGELKYDIVHSGVAVKQLQERSQTPVSNLVEPPSSACSEFSTLAPAPTQTDRQSGDDLLQQHLGELPEDILRELRMASASSEKGQVATPPVAIPYSPVVAHWFYKRSVDTKFIWTPFSHYDSALLETSLNLDDSTLIIPVEGGRYDVNIKERTKTPVYWEGKAIEVRRCSWFYKGVDSKYVPYTEDTAALLEAEYKRSAETGEWHQKIMLANGEQVVFHGPTVIVHFLPQQNADTWGASTQGSMRPRVVKRDLDDFTIEQGESQRVDHLLFMVHGIGSACDLKMRSVEEVVDDFRVIAQQLVQSHYKNSTDMGLVGRVEVLPISWHGHLHSEELGIDEKLKSITLESIPRLRNFTNDTLLDVLFYTSPKYCQKIMNTVADALNDVYLKYRMRHPEFNGGVSLAGHSLGSLILFDLLCHQEPLKESEEENKNPDQLPQKQSSQKVQLPTSDPLPKQVSYAMGPEGTGQPVITYTQLIFHPKKFFALGSPIGMFVTIRGIDKLGLDFHLPTCPGFYNIFHPFDPVAYRIEALVNPDMNGIRPVLIPHHKGRKRMHLELKETMTRVGADIKQRFMDTFKTTLDSVNFLTTVTKVKKEAEESLEKETSQTSSQAFQKQTEDQDESSVASSSCKLRNRTDSNSTTASDPEFIELDFPLGKLNDSKRVDYVLQEAPLEFINEYIFALSSHVCYWGSEDTILFVMKEIYASLGISTDSQVPQSSMTIERPVSHESSVSHSLLPM

Target ORF information:

RefSeq Version	NM_001298812.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatidic acid phospholipase A1, transcript variant D (PAPLA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298812.1

ORF Insert Sequence:

ATGTCGGAAA GATCGAAAGG TGACGTGTCC GCTCGCGTAA ACAACAACGC AAACCAGACG 
CGTGTCGTTA AAAATCCGCT TCTGAGCGCC TCCGTGACCG GCCTGAGTTT CGACGACTTC 
CCCAACAAGC CGCAGCTCCT GCCAGCCGCC CCGCCCGTGG TCCATGCCAC GCCTCCAGCG 
GCGCCCGTCC TCATCGCTGG CATCTTGAAT CGCGAACCGA AATCATTGGT CACCGTTGGT 
CAGCCATCGA GCATTGACGA TAGCGAGGCG CTCGACGAGA TTAACTTGAA TACCAGCAGC 
GAGGATTCCG TTGGCGCAGG TGGATCGAGT CCATATCTAG GAGCAGGCGA TCAAAACGCC 
AATCCTCTGT TGGAACCACC AACGGAAGCT GGAAACGATT CGTTGCTGAG CCAGGCGGCC 
TCCTCCTTTA CTGCCCTGCC TGCGGTGGCA TCCAATGTGT TTTCCACCTT CTCGAAGCGG 
ATCTACGCCG GCAGCAGGGA GACCTCGGAG GAGCCCAAAC TAGACATACA GCCCTCTTAC 
ATTCAGCAGG CCAGCTACGT TCAGCCAGTA GCTCCAGTGC CAGCTCCCTT TTATGCAGCA 
CCGCCAGCGG CCGCAAACGA AGTGGTCGCC CCCGAACCAC CCAAGTTCTA CGCACCTACT 
GAGGTTCCGC CACCGAATGT AGGAGTCCCT GCACCACCGC CTACATCGGG AAATCCAAAC 
ACATACAGAT TCACCGCCAG GAAGAAGCTA TACGCTCCCA TTCCTGGATT ATCCGAGCAG 
TCCGGACAAC CTGCACAACA GCCACAGGTT CCTCAGGCTC CCCAAGCGGC TCCATCATTC 
CAGACGCCAC CATATCCACC ACAGCTAACG TACGCAGCAA ACCCTGCTCC CTTTGACATA 
TCAGCGCAGC CAGCTGCAAT TCCCGCAGAG GAACGCAAAT CTGGTGGAGG ACTCTTCTCT 
TTGACCAGTC TCGTGCCCAC AGGAGTGCTT CAAAATATCA CAGGAATTGT TCAATCAGCC 
ACTGGGCGAA GCAGTGAACC AGAACACACT AACGTCCAGC AAGCAGGAGG CTACCTAGAC 
ATTACAACAG CATCAGCACC AGCTCCATCG ACCAACTTAT TTGGTGGACC GAATTCAACC 
TTTCCGCCGG GAGGAAATTA TTTCGTACCG GGAGCACCTC CATCATTACC CTCAACAGGG 
GTAGTAGGCC CCCCTTCGGT CGGAAACTTC TTCGCGCCGG CTGCTCCATT GCCAGTAGCA 
CAAACAGCTG CTCCACCAGC TGTCGGAGCC TTCTTCACAT CTGCTGCCCC AAGTGTTACG 
CCTTCAGTTT TTACTCCAGG AACAGTTCCA CCTCTTGCAC CGCAGCCTTT TCAGTCAGCA 
ACTGTAAACC AATCTGCATT GCCACCAAAC AATCCGCCGA CCGCATCTGC AGTAGGAGAA 
CTCTTTGTTC CAGCACCAAC GACAATCGCC CAAGGAACTT CACCTGCAGT TCCATTTGTT 
GACACACAAG CTGTTCCAAC AATACCTTTA ACAGTTCCAC CAGGCAATCC TCCTCCAACA 
TCGACACTTG GAGGATTCTT TACCACAGGT GAAGTTGCAC CTGTTGTTGC GCCATTTGGA 
ACACAAGCGT CACCAGCAAC ACAATCTGCA GGATTCTTTA ATCCAGGAGA AGTTCGTCCT 
AATGTCCCGT TAGCCGAACC TGTTCCAACT ATACCGATTA CATCATCAGG TGTTCCACCT 
ATAGGATTAT TTCCTCCAGC ACCGGCACAG CCTGGTGTCC AAACTGCTTC AAATCCGAAT 
CCCTTGCCAG GAGCAGCTGC ACCGCCACCA GCCGCTGGGC AAGCTTCATA TCGTCTCCAG 
AAGGGAACTC GTCTTTACAA GAGTCCATTG ACGCCCCAGG AAACCGCAAC ATCTTCGGGA 
TTCGCCGCTC CTCTGCCCCC CACATCTGTA ACTCCAGCAA TTTTTAATCC ATTCGGAGCA 
CCGGCACCAG GATCTTTCAA TTCAGTAGGC CATCTAGAGG GTCAATCCAG CCAAGGAGTT 
CCTTTCTTTA CGCCGCCAGT GCAGAATCCA CCAGGACAAG TAGCGGCTCC AACTGTTGCT 
GTTCCGCAAT CAGCACCTAT TGCCCCTCCA CCAGGGACAT CAAGCACAAG TGTTGCTCCT 
CCGCTAACAG CATCTATAGC CAATCCTCCT CCAACATCTG GCTTTGCAAG TGTACCCCTC 
TTCGCTGCAC CAACAATATC CTCAACGCCT ATTCCTTTCT TCAATCCTCA ACCAACAGGA 
GCAGAAGTAA CAACTGGTTT GAGCCAACAG GAAAACGAAG CTACAGCATC GACAACTTTG 
GAAGGTCTAG TGCAAGGACC TGCTCCAACA CTCTCTGCAA GTCAATTCTT TGATACGCCC 
TTGCCAACAG GAACCCAACC GGAGAATCAA GTAGCAAGTG TTTCCTCGGA AAGAGAAAAT 
CTTGTACAGG AGCCAATATC GAACCAAGGA ATTCCATTCT TTGCGCCGCC GCAAACGAAT 
ATTTTTACAC CACAGCTTCA GCAAGAGTTC ATAGCACCAG CAGAGGATTC CAAGCGATCC 
GAGATTCAAG AAGAATTCTT ATCAGCACCA CCACAAGAAT CTAATCTATC AACCGAACTT 
AGAGAAGATA CACACACTGG TCCACCCGTA ATCGAACCCG TTTTGGAAGA AACCCCAGCA 
AGTAGCTCGG AATCGAGTGT TCCGCCCGCA AGTAAGGTAG ATCAAGAAGA TATAGTCTAC 
CCAGAACCAT CTGCGCCGGT GGAACAAGAG ATTCCATTAT TCGTTCCAGT GCCAGGTTTG 
CCGGGACTAA GTCAGGACAT ACCACTATAT CCACCACCAC CAACTGGTTC CACCACTGCC 
AGCTCGATTT TTGCTGCGCC ACCAGTGGCT TCAAGTGCTC CACTTTTTGT TCCTGTTCCT 
GGTCCTCCGT CGGTTTCATC GTTATTTTCC CCCTCTCAAG TGGATTCTAC TTCTTCGTTA 
TTTGCTCCGC CACCAACATC TGCAAATACA ACAATACTAA CACCGGCTCC GGATACGACT 
TCTACGTTGT TTGCCAACAA CATACCAACA ACAACACCAT CCATACCAGA TATAGTTCCA 
ACTACATCGG CTGAGCTGGT TGACGCACCT CCTGGTTTCC AGGGATCATC AGACGACACC 
GCAACCAATC TATTTCAGGG AAACACCGAA ACCCAAACAG TTTTTAGTCC ATTTGCACAA 
ACTAAGGCTA CACCTTTGGT TCAGGAAACA AAACCCGAAT TGGTTCCACC TCCAATTGGA 
TTCTTTGCGC CGAAAAGCGA GTCGGAAGTT CTTAGCCAGC CACCACTTGC TCCCTCGCCA 
CTGCAAAGCT TCTTCAGCAC AGCACCAGAA ACAGCAACAA CCACCGACTT CAATTTCTTC 
GGAAGCCCAG CACCCGGCAG CGTGTTTCCT GATTTGCCAG TGCAGCAGGG AATCGCACCG 
CCACCATTGG CACTTGAGTC ACCGCCACAG AATAGTGTGT CCAATATAGC CACATCTGCT 
ATAGGATTCG ATTTGTTAAA CCAGACTCAG CAAGCTTCAA GTTTAGTCGA AAACTCAAAT 
CAAAATTCAA GTGTCAACTC TTTCGACGGC TATTTTGGTG GTCCTAGTGC ATCTGCGGCA 
CAAACATTCG CAAACGAGAC AACTAATGCA CTTGTTGCTC CAGCTATAAA CTGCGATCCC 
GTCAATTTCT TCGATCAGGT TCCTCAAACC CAGTCGCAAA TCCAGCAAGC AAACGAGGAT 
CAGCGCATTC AGAACTTCTT CAACAATCCA CCGTTGCAGG ATCAACCCGC TGTACCAGGT 
GAACTCAAAT ACGACATTGT CCACAGCGGA GTGGCTGTTA AGCAGCTGCA AGAGCGCTCA 
CAGACGCCCG TTTCAAATCT TGTCGAGCCA CCTTCGTCTG CCTGTTCGGA ATTCTCGACC 
CTTGCTCCAG CACCAACACA AACCGATCGC CAATCGGGCG ACGATCTACT CCAACAGCAT 
CTGGGCGAAT TGCCCGAGGA TATCCTCAGA GAGCTTAGAA TGGCAAGTGC CAGCAGCGAA 
AAGGGCCAGG TGGCGACGCC TCCCGTGGCT ATACCTTACT CACCAGTGGT GGCCCATTGG 
TTCTACAAGC GCAGCGTGGA CACAAAGTTC ATCTGGACAC CATTCAGCCA CTATGATTCG 
GCCTTGTTAG AAACCAGCCT AAATCTTGAC GATTCCACAT TGATTATTCC CGTTGAGGGT 
GGCCGCTACG ATGTAAACAT CAAGGAACGT ACCAAGACTC CCGTTTACTG GGAGGGAAAG 
GCCATCGAAG TGCGACGCTG CTCCTGGTTC TACAAGGGAG TTGACTCCAA GTATGTTCCA 
TACACCGAGG ATACGGCTGC ACTACTTGAA GCGGAATACA AGCGATCTGC GGAGACTGGT 
GAATGGCATC AGAAGATTAT GCTGGCCAAT GGCGAACAGG TGGTCTTCCA CGGACCTACA 
GTCATCGTTC ACTTCCTGCC GCAACAGAAT GCAGACACTT GGGGAGCCAG CACACAGGGT 
TCCATGCGTC CGAGAGTTGT CAAGAGGGAT CTGGATGATT TCACCATCGA GCAAGGCGAA 
TCGCAGCGAG TCGATCACCT GCTTTTCATG GTCCATGGCA TTGGCTCAGC CTGCGATTTG 
AAAATGCGTT CCGTGGAGGA AGTGGTGGAT GACTTCCGGG TTATTGCCCA GCAACTAGTT 
CAGTCGCACT ACAAAAACTC AACAGATATG GGCCTGGTGG GTCGCGTTGA AGTTTTGCCC 
ATCTCGTGGC ACGGACACCT GCACTCCGAG GAGTTAGGCA TCGACGAAAA GCTGAAATCC 
ATCACTTTGG AGTCTATTCC TCGCTTGCGA AACTTCACCA ATGACACGCT GCTCGACGTG 
CTGTTCTACA CCAGTCCGAA ATACTGTCAA AAGATCATGA ACACGGTGGC AGATGCGCTT 
AACGATGTCT ACCTGAAGTA CCGAATGCGG CACCCTGAAT TCAATGGGGG AGTTTCGCTG 
GCAGGTCACA GTCTCGGCTC CCTAATCCTA TTCGATTTAT TGTGCCATCA GGAACCGCTC 
AAGGAAAGCG AGGAAGAGAA TAAAAATCCC GACCAACTTC CCCAAAAGCA ATCGAGTCAA 
AAGGTACAGC TTCCAACCAG CGATCCTTTG CCGAAACAAG TCAGCTATGC GATGGGTCCG 
GAGGGCACGG GACAGCCCGT GATTACATAT ACACAGCTGA TATTCCACCC CAAAAAATTC 
TTTGCTCTGG GCTCACCCAT CGGAATGTTT GTAACCATCA GGGGGATTGA TAAGCTTGGT 
CTCGACTTTC ACCTGCCCAC GTGTCCGGGT TTCTACAACA TATTCCATCC CTTCGATCCG 
GTAGCCTATC GGATCGAGGC GCTTGTCAAT CCGGACATGA ACGGCATTCG ACCGGTGTTA 
ATTCCCCATC ACAAGGGACG AAAACGAATG CACCTGGAGC TGAAGGAGAC GATGACACGT 
GTGGGTGCGG ACATCAAGCA GCGATTTATG GACACATTCA AGACCACGCT GGACAGCGTA 
AACTTTTTGA CCACGGTAAC GAAGGTTAAG AAGGAAGCGG AGGAGTCGCT GGAGAAGGAG 
ACAAGCCAGA CTTCATCGCA AGCTTTTCAA AAGCAAACCG AGGATCAGGA CGAGAGTTCA 
GTTGCCAGTT CATCTTGCAA ACTACGAAAT CGCACCGATT CCAATTCGAC GACGGCTTCT 
GACCCTGAGT TTATAGAACT TGATTTCCCA CTGGGTAAGC TGAACGACTC AAAACGTGTG 
GACTACGTGC TTCAGGAAGC TCCACTAGAA TTCATCAACG AATATATTTT CGCCCTGAGC 
AGCCATGTGT GCTACTGGGG CTCCGAAGAT ACGATCCTTT TCGTGATGAA GGAGATTTAT 
GCCAGCCTGG GAATCAGCAC AGACAGCCAG GTGCCGCAGT CCTCAATGAC CATCGAGCGA 
CCCGTTTCGC ATGAGAGCAG TGTTAGCCAC TCGCTGTTGC CGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PAPLA1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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