baf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	baf
Entrez Gene ID	34095
Full Name	barrier to autointegration factor
Synonyms	BAF,CG7380,Dmel\CG7380,anon-WO0172774.178,dBAF
General protein information	Preferred Names barrier to autointegration factor Names CG7380 gene product from transcript CG7380-RB Barrier-to-autointegration factor CG7380-PA CG7380-PB baf-PA baf-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 28D3-28D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_135332.4	NP_609176.1	barrier to autointegration factor, isoform A
NM_001273291.1	NP_001260220.1	barrier to autointegration factor, isoform B

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	banf1	NP_991125.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008753	XP_314872.1
Rattus norvegicus (Norway rat)	Banf1	NP_446083.1
Drosophila melanogaster (fruit fly)	baf	NP_609176.1
Pan troglodytes (chimpanzee)	BANF1	XP_003339255.1
Canis lupus familiaris (dog)	BANF1	XP_854776.1
Xenopus tropicalis (tropical clawed frog)	banf1	XP_002940546.1
Homo sapiens (human)	BANF1	NP_003851.1
Mus musculus (house mouse)	Banf1	NP_001033320.1
Caenorhabditis elegans (roundworm)	baf-1	NP_499085.1
	BANF1	XP_001111884.2
Bos taurus (cattle)	BANF1	NP_892033.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BAF phosphorylation by NHK-1 disrupts anchorage of chromosomes to the nuclear envelope, allowing karyosome formation in oocytes.
Title: NHK-1 phosphorylates BAF to allow karyosome formation in the Drosophila oocyte nucleus.

BAF disappears at an early stage due to caspase activity when apoptosis is induced by hid, and its depletion in mutants is sufficient in itself to induce cell death, suggesting it is an apoptotic mediator
Title: BAF as a caspase-dependent mediator of nuclear apoptosis in Drosophila.

BAF plays essential roles in nuclear organization and that these BAF functions are required in both M phase and interphase of the cell cycle
Title: Barrier-to-autointegration factor plays crucial roles in cell cycle progression and nuclear organization in Drosophila.

The following baf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the baf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14077	NM_001273291.1 Latest version!	Drosophila melanogaster barrier to autointegration factor (baf), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa14077	NM_135332.4 Latest version!	Drosophila melanogaster barrier to autointegration factor (baf), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

baf ( NM_135332.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609176.1 Drosophila melanogaster barrier to autointegration factor (baf), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14077
Clone ID Related Accession (Same CDS sequence)	NM_135332.4 , NM_001273291.1
Accession Version	NM_135332.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	barrier to autointegration factor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135332.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGGCA CATCGCAGAA ACACAGGAAC TTCGTTGCGG AGCCAATGGG CAACAAGTCG 
GTGACGGAAC TGGCCGGAAT TGGGGAAACC CTCGGTGGAC GCTTGAAGGA CGCTGGATTC 
GATATGGCCT ACACCGTTTT GGGACAGTAT CTGGTGCTGA AAAAGGACGA GGAGCTGTTC 
AAGGACTGGA TGAAGGAGGT GTGCCACGCC AGCTCCAAAC AGGCATCCGA TTGCTACAAC 
TGTCTCAACG ATTGGTGCGA GGAGTTCTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609176.1
CDS	147..419
Translation	MSGTSQKHRNFVAEPMGNKSVTELAGIGETLGGRLKDAGFDMAYTVLGQYLVLKKDEELFKDWMKEVCHASSKQASDCYNCLNDWCEEFL

Target ORF information:

RefSeq Version	NM_135332.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster barrier to autointegration factor (baf), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135332.4

ORF Insert Sequence:

ATGTCGGGCA CATCGCAGAA ACACAGGAAC TTCGTTGCGG AGCCAATGGG CAACAAGTCG 
GTGACGGAAC TGGCCGGAAT TGGGGAAACC CTCGGTGGAC GCTTGAAGGA CGCTGGATTC 
GATATGGCCT ACACCGTTTT GGGACAGTAT CTGGTGCTGA AAAAGGACGA GGAGCTGTTC 
AAGGACTGGA TGAAGGAGGT GTGCCACGCC AGCTCCAAAC AGGCATCCGA TTGCTACAAC 
TGTCTCAACG ATTGGTGCGA GGAGTTCTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

baf ( NM_001273291.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260220.1 Drosophila melanogaster barrier to autointegration factor (baf), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14077
Clone ID Related Accession (Same CDS sequence)	NM_135332.4 , NM_001273291.1
Accession Version	NM_001273291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	barrier to autointegration factor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGGCA CATCGCAGAA ACACAGGAAC TTCGTTGCGG AGCCAATGGG CAACAAGTCG 
GTGACGGAAC TGGCCGGAAT TGGGGAAACC CTCGGTGGAC GCTTGAAGGA CGCTGGATTC 
GATATGGCCT ACACCGTTTT GGGACAGTAT CTGGTGCTGA AAAAGGACGA GGAGCTGTTC 
AAGGACTGGA TGAAGGAGGT GTGCCACGCC AGCTCCAAAC AGGCATCCGA TTGCTACAAC 
TGTCTCAACG ATTGGTGCGA GGAGTTCTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260220.1
CDS	147..419
Translation	MSGTSQKHRNFVAEPMGNKSVTELAGIGETLGGRLKDAGFDMAYTVLGQYLVLKKDEELFKDWMKEVCHASSKQASDCYNCLNDWCEEFL

Target ORF information:

RefSeq Version	NM_001273291.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster barrier to autointegration factor (baf), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273291.1

ORF Insert Sequence:

ATGTCGGGCA CATCGCAGAA ACACAGGAAC TTCGTTGCGG AGCCAATGGG CAACAAGTCG 
GTGACGGAAC TGGCCGGAAT TGGGGAAACC CTCGGTGGAC GCTTGAAGGA CGCTGGATTC 
GATATGGCCT ACACCGTTTT GGGACAGTAT CTGGTGCTGA AAAAGGACGA GGAGCTGTTC 
AAGGACTGGA TGAAGGAGGT GTGCCACGCC AGCTCCAAAC AGGCATCCGA TTGCTACAAC 
TGTCTCAACG ATTGGTGCGA GGAGTTCTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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baf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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