Spn28Dc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spn28Dc
Entrez Gene ID	34091
Full Name	Serpin 28Dc
Synonyms	CG7219,Dm-Spn28D,Dmel\CG7219,Spn28D,spn28D
General protein information	Preferred Names Serpin 28Dc Names CG7219 gene product from transcript CG7219-RA CG7219-PA Dm-serpin-28D Serpin-28D Spn28Dc-PA serpin 28D serpin 28Dc
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 28D3-28D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_135328.3	NP_609172.1	serpin 28Dc

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Spn28Dc	NP_609172.1
Anopheles gambiae (African malaria mosquito)	SRPN6	XP_319990.2
Anopheles gambiae (African malaria mosquito)	SRPN4	XP_001689142.1
Anopheles gambiae (African malaria mosquito)	SRPN16	XP_553153.2
Anopheles gambiae (African malaria mosquito)	SRPN5	XP_319998.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spn28D confines phenoloxidase availability by controlling its initial release, while Spn27A is rather limiting the melanization reaction to the wound site.
Title: Drosophila Serpin-28D regulates hemolymph phenoloxidase activity and adult pigmentation.

The following Spn28Dc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spn28Dc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14072	NM_135328.3 Latest version!	Drosophila melanogaster serpin 28Dc (Spn28Dc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spn28Dc ( NM_135328.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609172.1 Drosophila melanogaster serpin 28Dc (Spn28Dc), mRNA.

Price & Availability↑

CloneID	OFa14072
Clone ID Related Accession (Same CDS sequence)	NM_135328.3
Accession Version	NM_135328.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	serpin 28Dc
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135328.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGCGCT TGCTTTTGGC ACTTTTGCTC GTGAGTTCGG TGTGCTGTGA ATCGGAACTA 
TTTCGTGATG ACCTGAGGAC CCCGGAAACG ATGGCCTACA TCAATGGACT GATGCAGCGG 
CGGCATCAGA TGCAACAGGA GGCGCAGCAG CACATTCAGG CGATACCGCC TGCGGTGCCC 
CTTCAATCGC CCGGCCTTGT GAACGGCCTT GGCAACCAGA ATGACCCTGC GTTGAATCGC 
ATATCCGGCA CTTCGGTGAA ACCTTCCAAC CTTCCGGCTG CCTACTCCAA CGGCTATGTG 
GATCTGGCCA CCTCTGATCG GATTGCGAAC AGTGTGCTGA ACTTTGCCAA CATTCTGGGC 
CAGCATTTGG CCAACGGTAA AACCCAGATA TACTCTCCCC TGAGCATTGT ACACTCGCTG 
GCTCTGCTGC TCCTGGGCGC CAAGGGTCGC AGTTACGAGG AGTTGTCCAC GGTCTTCGAT 
ATTCCGGACA CGTCCAGGCT GCACGAACAG TTCGGCCTGA TGCTGCAGGA TCTGCAACAG 
CCCACCAGGG AGGCGATCTC CGCAGGACGA CCGCTGACCG ATTGGCGGGC GAGCAGCGCG 
ATGAGATCCA ATCGACGGGC CCAGCGACCC GGTGCTCATG AGGTGCACCT GGCCAATGGA 
CTGTTCACAC AGACCGGCTA CACACTCAAT CCCGACTACA GACGGGTGAT CGTCGAGGTC 
TACGCCTCGG ATCTGCAGAT TCAGGATTTC GAGGGCAGTC CGGCGACAGC ACGATACAAT 
ATCAATGCTT ATGTGGCGCA GCACACAAAG AATCACATCG AGAACATCAT CGCCAGCGAT 
ATTCCGCAGA CCACCAGGAT GATTCTGGCC AACGCATTGT ACTTTAAGGC ATTCTGGGAA 
ACGGACTTTA TAGAGTCAGC CACACGTCCG GATAACTTCT ATCCGAATGG CGAGGGAACG 
GAGCCGGTGA TGAGGGTGCA GATGATGGCC ACCGGTGGTG CATATCCGTA TCACGAGGAT 
CACGAGCTGG GCTGCAAGAT CATTGGACTG CCGTACAGGG GAAATCTGAG CACCATGTAC 
ATCATCCAGC CGTTTAAGTC GTCGGTGAGG GAGCTAATGG CACTGCAGAA ACGTCTGACG 
GCGGATAAGA TCGAGAGCAT GATCAGCCGA ATGTACAGAC GGGCCGCTCT GGTGGCATTC 
CCAAAGATGC ATCTTACGGA GTCGGTGAAC TTGAAGACAG TCATGCAGCG AATGGGGCTG 
GGCGGCATCT TTAGCGCGGT GCAGAATGAT CTCTCGCTCA TCGCCACCAA CGAGGCCACG 
AGGACGAATG CGCTGGGCGG CAACAGCCTG CAGAATCTGG AGGCACAGCG CAGAGCCGGA 
ACGGGTGGAG CACGTTCCGA TCTGGTGGTG GATGACATTG TTCACAAAGT GGACTTCACC 
GTCAACGAGC AGGGCACGGA GGCGGCGGCG TCTTCGGTCA CATACCTCAA GAAATCCGGT 
CCCGATGTCC TCTTCCGCGG AGACACTCCA TTCATGGTCC TTGTGCGCCA CGATCCCACG 
AAACTGGTGC TCTTCTACGG ACTCATAAAT GAGCCCCCGG CGGCCGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609172.1
CDS	79..1689
Translation	MWRLLLALLLVSSVCCESELFRDDLRTPETMAYINGLMQRRHQMQQEAQQHIQAIPPAVPLQSPGLVNGLGNQNDPALNRISGTSVKPSNLPAAYSNGYVDLATSDRIANSVLNFANILGQHLANGKTQIYSPLSIVHSLALLLLGAKGRSYEELSTVFDIPDTSRLHEQFGLMLQDLQQPTREAISAGRPLTDWRASSAMRSNRRAQRPGAHEVHLANGLFTQTGYTLNPDYRRVIVEVYASDLQIQDFEGSPATARYNINAYVAQHTKNHIENIIASDIPQTTRMILANALYFKAFWETDFIESATRPDNFYPNGEGTEPVMRVQMMATGGAYPYHEDHELGCKIIGLPYRGNLSTMYIIQPFKSSVRELMALQKRLTADKIESMISRMYRRAALVAFPKMHLTESVNLKTVMQRMGLGGIFSAVQNDLSLIATNEATRTNALGGNSLQNLEAQRRAGTGGARSDLVVDDIVHKVDFTVNEQGTEAAASSVTYLKKSGPDVLFRGDTPFMVLVRHDPTKLVLFYGLINEPPAAA

Target ORF information:

RefSeq Version	NM_135328.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serpin 28Dc (Spn28Dc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135328.3

ORF Insert Sequence:

ATGTGGCGCT TGCTTTTGGC ACTTTTGCTC GTGAGTTCGG TGTGCTGTGA ATCGGAACTA 
TTTCGTGATG ACCTGAGGAC CCCGGAAACG ATGGCCTACA TCAATGGACT GATGCAGCGG 
CGGCATCAGA TGCAACAGGA GGCGCAGCAG CACATTCAGG CGATACCGCC TGCGGTGCCC 
CTTCAATCGC CCGGCCTTGT GAACGGCCTT GGCAACCAGA ATGACCCTGC GTTGAATCGC 
ATATCCGGCA CTTCGGTGAA ACCTTCCAAC CTTCCGGCTG CCTACTCCAA CGGCTATGTG 
GATCTGGCCA CCTCTGATCG GATTGCGAAC AGTGTGCTGA ACTTTGCCAA CATTCTGGGC 
CAGCATTTGG CCAACGGTAA AACCCAGATA TACTCTCCCC TGAGCATTGT ACACTCGCTG 
GCTCTGCTGC TCCTGGGCGC CAAGGGTCGC AGTTACGAGG AGTTGTCCAC GGTCTTCGAT 
ATTCCGGACA CGTCCAGGCT GCACGAACAG TTCGGCCTGA TGCTGCAGGA TCTGCAACAG 
CCCACCAGGG AGGCGATCTC CGCAGGACGA CCGCTGACCG ATTGGCGGGC GAGCAGCGCG 
ATGAGATCCA ATCGACGGGC CCAGCGACCC GGTGCTCATG AGGTGCACCT GGCCAATGGA 
CTGTTCACAC AGACCGGCTA CACACTCAAT CCCGACTACA GACGGGTGAT CGTCGAGGTC 
TACGCCTCGG ATCTGCAGAT TCAGGATTTC GAGGGCAGTC CGGCGACAGC ACGATACAAT 
ATCAATGCTT ATGTGGCGCA GCACACAAAG AATCACATCG AGAACATCAT CGCCAGCGAT 
ATTCCGCAGA CCACCAGGAT GATTCTGGCC AACGCATTGT ACTTTAAGGC ATTCTGGGAA 
ACGGACTTTA TAGAGTCAGC CACACGTCCG GATAACTTCT ATCCGAATGG CGAGGGAACG 
GAGCCGGTGA TGAGGGTGCA GATGATGGCC ACCGGTGGTG CATATCCGTA TCACGAGGAT 
CACGAGCTGG GCTGCAAGAT CATTGGACTG CCGTACAGGG GAAATCTGAG CACCATGTAC 
ATCATCCAGC CGTTTAAGTC GTCGGTGAGG GAGCTAATGG CACTGCAGAA ACGTCTGACG 
GCGGATAAGA TCGAGAGCAT GATCAGCCGA ATGTACAGAC GGGCCGCTCT GGTGGCATTC 
CCAAAGATGC ATCTTACGGA GTCGGTGAAC TTGAAGACAG TCATGCAGCG AATGGGGCTG 
GGCGGCATCT TTAGCGCGGT GCAGAATGAT CTCTCGCTCA TCGCCACCAA CGAGGCCACG 
AGGACGAATG CGCTGGGCGG CAACAGCCTG CAGAATCTGG AGGCACAGCG CAGAGCCGGA 
ACGGGTGGAG CACGTTCCGA TCTGGTGGTG GATGACATTG TTCACAAAGT GGACTTCACC 
GTCAACGAGC AGGGCACGGA GGCGGCGGCG TCTTCGGTCA CATACCTCAA GAAATCCGGT 
CCCGATGTCC TCTTCCGCGG AGACACTCCA TTCATGGTCC TTGTGCGCCA CGATCCCACG 
AAACTGGTGC TCTTCTACGG ACTCATAAAT GAGCCCCCGG CGGCCGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Spn28Dc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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