CG7227 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG7227
Entrez Gene ID	34088
Full Name	CG7227 gene product from transcript CG7227-RC
Synonyms	Dmel\CG7227
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 28D3-28D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_135325.5	NP_609169.1	uncharacterized protein, isoform A
NM_001298804.1	NP_001285733.1	uncharacterized protein, isoform C
NM_001259005.2	NP_001245934.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG7227 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG7227 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14068	NM_001298804.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG7227), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14068	NM_001259005.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG7227), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa14068	NM_135325.5 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG7227), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG7227 ( NM_135325.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609169.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG7227), mRNA.

Price & Availability↑

CloneID	OFa14068
Clone ID Related Accession (Same CDS sequence)	NM_135325.5 , NM_001298804.1 , NM_001259005.2
Accession Version	NM_135325.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135325.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGGAA AACAGCGCAT TTGGCAGACA AGTGTGTTGG GTCTGCTTTT GGCCAGCTTC 
AGTCTGTTCT CCGTGCTCTA TATGGAGCAA ATCGAACGCT GGATGCTGGA GTGGTTCATG 
GTGCTCCGCC CAGGAACAAT GATCAGCAGC CTGTGGCAGT CACCGGCCAT GGACATAAAC 
GTTGACCTGT ACATATTCAA CTGGACGAAC TCGGAGAAGT TCAGTGATCC CACAGTGAAG 
CCCAGGTTCG AGGAGCTTGG TCCGTATCGC TTCACCGAAC GGATGCAGAA GGTCAATGTG 
GAGTGGCACG ATGAGAACTC GACGGTTTCC TATCGACGTC GCAGTCGATT CGATTTCGAT 
CCGAAACTTA GTGTCGGACG TCCTACAGAT CCCATTGTGG CGCCCAACCT GCTCATTGTG 
GGACTGTACC AGAAGATGGT CATGTGGAGT CCCATGCTGC GTTCCCTCAT GCTCTTGGCC 
CTGAATATCT ACGGCAAGGA GCAGGCCATG ATACGGCCAG CCAGCGATTG GATGTTCGAC 
GGCTTCGACA CGCCGATGAT CAAGATGAGC AAAATGGTGC CACCAAGTCT TGTGCCGGAG 
ATGAAGTTCC CCTATGAGAA GATCGGCTAT GCGTATCCAC GCAATGGCAG CATGGAAATC 
TACGGGCATC ACAATGTGTA CACGGGACGG GATGAGTTCC AAAAACTGGG TCAAATAGCC 
AGGTGGCGCT ACAACAATGT CACTGAGGCC AGTCCCAGGT GCAAGTTGAA GGGCAGCGCC 
GGAGAATTCC ATCCAATACC GCTGGTAAAG GGCAGACCCA TCTCCTACTT CCTGCCGGAT 
CTGTGCCGTG AGCTGCAGGT GGACTACTCC GGCACCACAA TCTTCGAGGG CATCGATGCT 
TTCGTTTACA GAGGCAGTGC ACGCAATATG GCAAATGGTA CTGATAACCC GGATAACAGT 
TGCTATTGCC AGGATAACTG CCAGGAGGTG AGATCCGGTC TTCTCAACAT ATCCTCCTGT 
TGGTATGGAA CGCCTGTCTT TGCTTCGTAT CCGCACTTCT TCAAAGCTGA TCCCTACTAC 
GGAGAGCAGG TGGAGGGCAT GAAGCCCGAC AAGGATCGCC ACGAGATGGT CATTATGTTG 
GAGCCCAAAA CGGGAATGGT GCTGGAGATC AAGGCGCGCA TTATGGCCAA CTTGCTGGTG 
GAGCCCAAAA CTCATTTAAT TTACCGGACG GCGAGAAGAA CATTCTTTCC GTTGATCTGG 
GCGGATTACA ATGTCCGCAT CACGGATGAT CTTCTGGTCT ACGTGAAGCT AATGCCGATC 
CTTGAGTTTG TGGGCAAGAT CTGTGGCATC CTGGGCGTGG TCTTGGGTTT GCTGATGGTA 
TTCTGGTATC CCCATCAAAT GATCTGGCAG AAGAGCCTGA TGCACAAGAT CGAGATTAGT 
TCCATCGAGA CGAATAGCTC GTTGGAGCCT GTGAAGAATA ATGATGTGGA GGACTCGCCA 
CTGCTGAAGG GATTGCAATA TGCAGCGGCC AAAGATGGGG CGGGAAATGG AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609169.1
CDS	545..2101
Translation	MIGKQRIWQTSVLGLLLASFSLFSVLYMEQIERWMLEWFMVLRPGTMISSLWQSPAMDINVDLYIFNWTNSEKFSDPTVKPRFEELGPYRFTERMQKVNVEWHDENSTVSYRRRSRFDFDPKLSVGRPTDPIVAPNLLIVGLYQKMVMWSPMLRSLMLLALNIYGKEQAMIRPASDWMFDGFDTPMIKMSKMVPPSLVPEMKFPYEKIGYAYPRNGSMEIYGHHNVYTGRDEFQKLGQIARWRYNNVTEASPRCKLKGSAGEFHPIPLVKGRPISYFLPDLCRELQVDYSGTTIFEGIDAFVYRGSARNMANGTDNPDNSCYCQDNCQEVRSGLLNISSCWYGTPVFASYPHFFKADPYYGEQVEGMKPDKDRHEMVIMLEPKTGMVLEIKARIMANLLVEPKTHLIYRTARRTFFPLIWADYNVRITDDLLVYVKLMPILEFVGKICGILGVVLGLLMVFWYPHQMIWQKSLMHKIEISSIETNSSLEPVKNNDVEDSPLLKGLQYAAAKDGAGNGN

Target ORF information:

RefSeq Version	NM_135325.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG7227), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135325.5

ORF Insert Sequence:

ATGATTGGAA AACAGCGCAT TTGGCAGACA AGTGTGTTGG GTCTGCTTTT GGCCAGCTTC 
AGTCTGTTCT CCGTGCTCTA TATGGAGCAA ATCGAACGCT GGATGCTGGA GTGGTTCATG 
GTGCTCCGCC CAGGAACAAT GATCAGCAGC CTGTGGCAGT CACCGGCCAT GGACATAAAC 
GTTGACCTGT ACATATTCAA CTGGACGAAC TCGGAGAAGT TCAGTGATCC CACAGTGAAG 
CCCAGGTTCG AGGAGCTTGG TCCGTATCGC TTCACCGAAC GGATGCAGAA GGTCAATGTG 
GAGTGGCACG ATGAGAACTC GACGGTTTCC TATCGACGTC GCAGTCGATT CGATTTCGAT 
CCGAAACTTA GTGTCGGACG TCCTACAGAT CCCATTGTGG CGCCCAACCT GCTCATTGTG 
GGACTGTACC AGAAGATGGT CATGTGGAGT CCCATGCTGC GTTCCCTCAT GCTCTTGGCC 
CTGAATATCT ACGGCAAGGA GCAGGCCATG ATACGGCCAG CCAGCGATTG GATGTTCGAC 
GGCTTCGACA CGCCGATGAT CAAGATGAGC AAAATGGTGC CACCAAGTCT TGTGCCGGAG 
ATGAAGTTCC CCTATGAGAA GATCGGCTAT GCGTATCCAC GCAATGGCAG CATGGAAATC 
TACGGGCATC ACAATGTGTA CACGGGACGG GATGAGTTCC AAAAACTGGG TCAAATAGCC 
AGGTGGCGCT ACAACAATGT CACTGAGGCC AGTCCCAGGT GCAAGTTGAA GGGCAGCGCC 
GGAGAATTCC ATCCAATACC GCTGGTAAAG GGCAGACCCA TCTCCTACTT CCTGCCGGAT 
CTGTGCCGTG AGCTGCAGGT GGACTACTCC GGCACCACAA TCTTCGAGGG CATCGATGCT 
TTCGTTTACA GAGGCAGTGC ACGCAATATG GCAAATGGTA CTGATAACCC GGATAACAGT 
TGCTATTGCC AGGATAACTG CCAGGAGGTG AGATCCGGTC TTCTCAACAT ATCCTCCTGT 
TGGTATGGAA CGCCTGTCTT TGCTTCGTAT CCGCACTTCT TCAAAGCTGA TCCCTACTAC 
GGAGAGCAGG TGGAGGGCAT GAAGCCCGAC AAGGATCGCC ACGAGATGGT CATTATGTTG 
GAGCCCAAAA CGGGAATGGT GCTGGAGATC AAGGCGCGCA TTATGGCCAA CTTGCTGGTG 
GAGCCCAAAA CTCATTTAAT TTACCGGACG GCGAGAAGAA CATTCTTTCC GTTGATCTGG 
GCGGATTACA ATGTCCGCAT CACGGATGAT CTTCTGGTCT ACGTGAAGCT AATGCCGATC 
CTTGAGTTTG TGGGCAAGAT CTGTGGCATC CTGGGCGTGG TCTTGGGTTT GCTGATGGTA 
TTCTGGTATC CCCATCAAAT GATCTGGCAG AAGAGCCTGA TGCACAAGAT CGAGATTAGT 
TCCATCGAGA CGAATAGCTC GTTGGAGCCT GTGAAGAATA ATGATGTGGA GGACTCGCCA 
CTGCTGAAGG GATTGCAATA TGCAGCGGCC AAAGATGGGG CGGGAAATGG AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG7227 ( NM_001298804.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285733.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG7227), mRNA.

Price & Availability↑

CloneID	OFa14068
Clone ID Related Accession (Same CDS sequence)	NM_135325.5 , NM_001298804.1 , NM_001259005.2
Accession Version	NM_001298804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGGAA AACAGCGCAT TTGGCAGACA AGTGTGTTGG GTCTGCTTTT GGCCAGCTTC 
AGTCTGTTCT CCGTGCTCTA TATGGAGCAA ATCGAACGCT GGATGCTGGA GTGGTTCATG 
GTGCTCCGCC CAGGAACAAT GATCAGCAGC CTGTGGCAGT CACCGGCCAT GGACATAAAC 
GTTGACCTGT ACATATTCAA CTGGACGAAC TCGGAGAAGT TCAGTGATCC CACAGTGAAG 
CCCAGGTTCG AGGAGCTTGG TCCGTATCGC TTCACCGAAC GGATGCAGAA GGTCAATGTG 
GAGTGGCACG ATGAGAACTC GACGGTTTCC TATCGACGTC GCAGTCGATT CGATTTCGAT 
CCGAAACTTA GTGTCGGACG TCCTACAGAT CCCATTGTGG CGCCCAACCT GCTCATTGTG 
GGACTGTACC AGAAGATGGT CATGTGGAGT CCCATGCTGC GTTCCCTCAT GCTCTTGGCC 
CTGAATATCT ACGGCAAGGA GCAGGCCATG ATACGGCCAG CCAGCGATTG GATGTTCGAC 
GGCTTCGACA CGCCGATGAT CAAGATGAGC AAAATGGTGC CACCAAGTCT TGTGCCGGAG 
ATGAAGTTCC CCTATGAGAA GATCGGCTAT GCGTATCCAC GCAATGGCAG CATGGAAATC 
TACGGGCATC ACAATGTGTA CACGGGACGG GATGAGTTCC AAAAACTGGG TCAAATAGCC 
AGGTGGCGCT ACAACAATGT CACTGAGGCC AGTCCCAGGT GCAAGTTGAA GGGCAGCGCC 
GGAGAATTCC ATCCAATACC GCTGGTAAAG GGCAGACCCA TCTCCTACTT CCTGCCGGAT 
CTGTGCCGTG AGCTGCAGGT GGACTACTCC GGCACCACAA TCTTCGAGGG CATCGATGCT 
TTCGTTTACA GAGGCAGTGC ACGCAATATG GCAAATGGTA CTGATAACCC GGATAACAGT 
TGCTATTGCC AGGATAACTG CCAGGAGGTG AGATCCGGTC TTCTCAACAT ATCCTCCTGT 
TGGTATGGAA CGCCTGTCTT TGCTTCGTAT CCGCACTTCT TCAAAGCTGA TCCCTACTAC 
GGAGAGCAGG TGGAGGGCAT GAAGCCCGAC AAGGATCGCC ACGAGATGGT CATTATGTTG 
GAGCCCAAAA CGGGAATGGT GCTGGAGATC AAGGCGCGCA TTATGGCCAA CTTGCTGGTG 
GAGCCCAAAA CTCATTTAAT TTACCGGACG GCGAGAAGAA CATTCTTTCC GTTGATCTGG 
GCGGATTACA ATGTCCGCAT CACGGATGAT CTTCTGGTCT ACGTGAAGCT AATGCCGATC 
CTTGAGTTTG TGGGCAAGAT CTGTGGCATC CTGGGCGTGG TCTTGGGTTT GCTGATGGTA 
TTCTGGTATC CCCATCAAAT GATCTGGCAG AAGAGCCTGA TGCACAAGAT CGAGATTAGT 
TCCATCGAGA CGAATAGCTC GTTGGAGCCT GTGAAGAATA ATGATGTGGA GGACTCGCCA 
CTGCTGAAGG GATTGCAATA TGCAGCGGCC AAAGATGGGG CGGGAAATGG AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285733.1
CDS	403..1959
Translation	MIGKQRIWQTSVLGLLLASFSLFSVLYMEQIERWMLEWFMVLRPGTMISSLWQSPAMDINVDLYIFNWTNSEKFSDPTVKPRFEELGPYRFTERMQKVNVEWHDENSTVSYRRRSRFDFDPKLSVGRPTDPIVAPNLLIVGLYQKMVMWSPMLRSLMLLALNIYGKEQAMIRPASDWMFDGFDTPMIKMSKMVPPSLVPEMKFPYEKIGYAYPRNGSMEIYGHHNVYTGRDEFQKLGQIARWRYNNVTEASPRCKLKGSAGEFHPIPLVKGRPISYFLPDLCRELQVDYSGTTIFEGIDAFVYRGSARNMANGTDNPDNSCYCQDNCQEVRSGLLNISSCWYGTPVFASYPHFFKADPYYGEQVEGMKPDKDRHEMVIMLEPKTGMVLEIKARIMANLLVEPKTHLIYRTARRTFFPLIWADYNVRITDDLLVYVKLMPILEFVGKICGILGVVLGLLMVFWYPHQMIWQKSLMHKIEISSIETNSSLEPVKNNDVEDSPLLKGLQYAAAKDGAGNGN

Target ORF information:

RefSeq Version	NM_001298804.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG7227), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298804.1

ORF Insert Sequence:

ATGATTGGAA AACAGCGCAT TTGGCAGACA AGTGTGTTGG GTCTGCTTTT GGCCAGCTTC 
AGTCTGTTCT CCGTGCTCTA TATGGAGCAA ATCGAACGCT GGATGCTGGA GTGGTTCATG 
GTGCTCCGCC CAGGAACAAT GATCAGCAGC CTGTGGCAGT CACCGGCCAT GGACATAAAC 
GTTGACCTGT ACATATTCAA CTGGACGAAC TCGGAGAAGT TCAGTGATCC CACAGTGAAG 
CCCAGGTTCG AGGAGCTTGG TCCGTATCGC TTCACCGAAC GGATGCAGAA GGTCAATGTG 
GAGTGGCACG ATGAGAACTC GACGGTTTCC TATCGACGTC GCAGTCGATT CGATTTCGAT 
CCGAAACTTA GTGTCGGACG TCCTACAGAT CCCATTGTGG CGCCCAACCT GCTCATTGTG 
GGACTGTACC AGAAGATGGT CATGTGGAGT CCCATGCTGC GTTCCCTCAT GCTCTTGGCC 
CTGAATATCT ACGGCAAGGA GCAGGCCATG ATACGGCCAG CCAGCGATTG GATGTTCGAC 
GGCTTCGACA CGCCGATGAT CAAGATGAGC AAAATGGTGC CACCAAGTCT TGTGCCGGAG 
ATGAAGTTCC CCTATGAGAA GATCGGCTAT GCGTATCCAC GCAATGGCAG CATGGAAATC 
TACGGGCATC ACAATGTGTA CACGGGACGG GATGAGTTCC AAAAACTGGG TCAAATAGCC 
AGGTGGCGCT ACAACAATGT CACTGAGGCC AGTCCCAGGT GCAAGTTGAA GGGCAGCGCC 
GGAGAATTCC ATCCAATACC GCTGGTAAAG GGCAGACCCA TCTCCTACTT CCTGCCGGAT 
CTGTGCCGTG AGCTGCAGGT GGACTACTCC GGCACCACAA TCTTCGAGGG CATCGATGCT 
TTCGTTTACA GAGGCAGTGC ACGCAATATG GCAAATGGTA CTGATAACCC GGATAACAGT 
TGCTATTGCC AGGATAACTG CCAGGAGGTG AGATCCGGTC TTCTCAACAT ATCCTCCTGT 
TGGTATGGAA CGCCTGTCTT TGCTTCGTAT CCGCACTTCT TCAAAGCTGA TCCCTACTAC 
GGAGAGCAGG TGGAGGGCAT GAAGCCCGAC AAGGATCGCC ACGAGATGGT CATTATGTTG 
GAGCCCAAAA CGGGAATGGT GCTGGAGATC AAGGCGCGCA TTATGGCCAA CTTGCTGGTG 
GAGCCCAAAA CTCATTTAAT TTACCGGACG GCGAGAAGAA CATTCTTTCC GTTGATCTGG 
GCGGATTACA ATGTCCGCAT CACGGATGAT CTTCTGGTCT ACGTGAAGCT AATGCCGATC 
CTTGAGTTTG TGGGCAAGAT CTGTGGCATC CTGGGCGTGG TCTTGGGTTT GCTGATGGTA 
TTCTGGTATC CCCATCAAAT GATCTGGCAG AAGAGCCTGA TGCACAAGAT CGAGATTAGT 
TCCATCGAGA CGAATAGCTC GTTGGAGCCT GTGAAGAATA ATGATGTGGA GGACTCGCCA 
CTGCTGAAGG GATTGCAATA TGCAGCGGCC AAAGATGGGG CGGGAAATGG AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG7227 ( NM_001259005.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245934.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG7227), mRNA.

Price & Availability↑

CloneID	OFa14068
Clone ID Related Accession (Same CDS sequence)	NM_135325.5 , NM_001298804.1 , NM_001259005.2
Accession Version	NM_001259005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259005.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTGGAA AACAGCGCAT TTGGCAGACA AGTGTGTTGG GTCTGCTTTT GGCCAGCTTC 
AGTCTGTTCT CCGTGCTCTA TATGGAGCAA ATCGAACGCT GGATGCTGGA GTGGTTCATG 
GTGCTCCGCC CAGGAACAAT GATCAGCAGC CTGTGGCAGT CACCGGCCAT GGACATAAAC 
GTTGACCTGT ACATATTCAA CTGGACGAAC TCGGAGAAGT TCAGTGATCC CACAGTGAAG 
CCCAGGTTCG AGGAGCTTGG TCCGTATCGC TTCACCGAAC GGATGCAGAA GGTCAATGTG 
GAGTGGCACG ATGAGAACTC GACGGTTTCC TATCGACGTC GCAGTCGATT CGATTTCGAT 
CCGAAACTTA GTGTCGGACG TCCTACAGAT CCCATTGTGG CGCCCAACCT GCTCATTGTG 
GGACTGTACC AGAAGATGGT CATGTGGAGT CCCATGCTGC GTTCCCTCAT GCTCTTGGCC 
CTGAATATCT ACGGCAAGGA GCAGGCCATG ATACGGCCAG CCAGCGATTG GATGTTCGAC 
GGCTTCGACA CGCCGATGAT CAAGATGAGC AAAATGGTGC CACCAAGTCT TGTGCCGGAG 
ATGAAGTTCC CCTATGAGAA GATCGGCTAT GCGTATCCAC GCAATGGCAG CATGGAAATC 
TACGGGCATC ACAATGTGTA CACGGGACGG GATGAGTTCC AAAAACTGGG TCAAATAGCC 
AGGTGGCGCT ACAACAATGT CACTGAGGCC AGTCCCAGGT GCAAGTTGAA GGGCAGCGCC 
GGAGAATTCC ATCCAATACC GCTGGTAAAG GGCAGACCCA TCTCCTACTT CCTGCCGGAT 
CTGTGCCGTG AGCTGCAGGT GGACTACTCC GGCACCACAA TCTTCGAGGG CATCGATGCT 
TTCGTTTACA GAGGCAGTGC ACGCAATATG GCAAATGGTA CTGATAACCC GGATAACAGT 
TGCTATTGCC AGGATAACTG CCAGGAGGTG AGATCCGGTC TTCTCAACAT ATCCTCCTGT 
TGGTATGGAA CGCCTGTCTT TGCTTCGTAT CCGCACTTCT TCAAAGCTGA TCCCTACTAC 
GGAGAGCAGG TGGAGGGCAT GAAGCCCGAC AAGGATCGCC ACGAGATGGT CATTATGTTG 
GAGCCCAAAA CGGGAATGGT GCTGGAGATC AAGGCGCGCA TTATGGCCAA CTTGCTGGTG 
GAGCCCAAAA CTCATTTAAT TTACCGGACG GCGAGAAGAA CATTCTTTCC GTTGATCTGG 
GCGGATTACA ATGTCCGCAT CACGGATGAT CTTCTGGTCT ACGTGAAGCT AATGCCGATC 
CTTGAGTTTG TGGGCAAGAT CTGTGGCATC CTGGGCGTGG TCTTGGGTTT GCTGATGGTA 
TTCTGGTATC CCCATCAAAT GATCTGGCAG AAGAGCCTGA TGCACAAGAT CGAGATTAGT 
TCCATCGAGA CGAATAGCTC GTTGGAGCCT GTGAAGAATA ATGATGTGGA GGACTCGCCA 
CTGCTGAAGG GATTGCAATA TGCAGCGGCC AAAGATGGGG CGGGAAATGG AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245934.1
CDS	403..1959
Translation	MIGKQRIWQTSVLGLLLASFSLFSVLYMEQIERWMLEWFMVLRPGTMISSLWQSPAMDINVDLYIFNWTNSEKFSDPTVKPRFEELGPYRFTERMQKVNVEWHDENSTVSYRRRSRFDFDPKLSVGRPTDPIVAPNLLIVGLYQKMVMWSPMLRSLMLLALNIYGKEQAMIRPASDWMFDGFDTPMIKMSKMVPPSLVPEMKFPYEKIGYAYPRNGSMEIYGHHNVYTGRDEFQKLGQIARWRYNNVTEASPRCKLKGSAGEFHPIPLVKGRPISYFLPDLCRELQVDYSGTTIFEGIDAFVYRGSARNMANGTDNPDNSCYCQDNCQEVRSGLLNISSCWYGTPVFASYPHFFKADPYYGEQVEGMKPDKDRHEMVIMLEPKTGMVLEIKARIMANLLVEPKTHLIYRTARRTFFPLIWADYNVRITDDLLVYVKLMPILEFVGKICGILGVVLGLLMVFWYPHQMIWQKSLMHKIEISSIETNSSLEPVKNNDVEDSPLLKGLQYAAAKDGAGNGN

Target ORF information:

RefSeq Version	NM_001259005.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG7227), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259005.2

ORF Insert Sequence:

ATGATTGGAA AACAGCGCAT TTGGCAGACA AGTGTGTTGG GTCTGCTTTT GGCCAGCTTC 
AGTCTGTTCT CCGTGCTCTA TATGGAGCAA ATCGAACGCT GGATGCTGGA GTGGTTCATG 
GTGCTCCGCC CAGGAACAAT GATCAGCAGC CTGTGGCAGT CACCGGCCAT GGACATAAAC 
GTTGACCTGT ACATATTCAA CTGGACGAAC TCGGAGAAGT TCAGTGATCC CACAGTGAAG 
CCCAGGTTCG AGGAGCTTGG TCCGTATCGC TTCACCGAAC GGATGCAGAA GGTCAATGTG 
GAGTGGCACG ATGAGAACTC GACGGTTTCC TATCGACGTC GCAGTCGATT CGATTTCGAT 
CCGAAACTTA GTGTCGGACG TCCTACAGAT CCCATTGTGG CGCCCAACCT GCTCATTGTG 
GGACTGTACC AGAAGATGGT CATGTGGAGT CCCATGCTGC GTTCCCTCAT GCTCTTGGCC 
CTGAATATCT ACGGCAAGGA GCAGGCCATG ATACGGCCAG CCAGCGATTG GATGTTCGAC 
GGCTTCGACA CGCCGATGAT CAAGATGAGC AAAATGGTGC CACCAAGTCT TGTGCCGGAG 
ATGAAGTTCC CCTATGAGAA GATCGGCTAT GCGTATCCAC GCAATGGCAG CATGGAAATC 
TACGGGCATC ACAATGTGTA CACGGGACGG GATGAGTTCC AAAAACTGGG TCAAATAGCC 
AGGTGGCGCT ACAACAATGT CACTGAGGCC AGTCCCAGGT GCAAGTTGAA GGGCAGCGCC 
GGAGAATTCC ATCCAATACC GCTGGTAAAG GGCAGACCCA TCTCCTACTT CCTGCCGGAT 
CTGTGCCGTG AGCTGCAGGT GGACTACTCC GGCACCACAA TCTTCGAGGG CATCGATGCT 
TTCGTTTACA GAGGCAGTGC ACGCAATATG GCAAATGGTA CTGATAACCC GGATAACAGT 
TGCTATTGCC AGGATAACTG CCAGGAGGTG AGATCCGGTC TTCTCAACAT ATCCTCCTGT 
TGGTATGGAA CGCCTGTCTT TGCTTCGTAT CCGCACTTCT TCAAAGCTGA TCCCTACTAC 
GGAGAGCAGG TGGAGGGCAT GAAGCCCGAC AAGGATCGCC ACGAGATGGT CATTATGTTG 
GAGCCCAAAA CGGGAATGGT GCTGGAGATC AAGGCGCGCA TTATGGCCAA CTTGCTGGTG 
GAGCCCAAAA CTCATTTAAT TTACCGGACG GCGAGAAGAA CATTCTTTCC GTTGATCTGG 
GCGGATTACA ATGTCCGCAT CACGGATGAT CTTCTGGTCT ACGTGAAGCT AATGCCGATC 
CTTGAGTTTG TGGGCAAGAT CTGTGGCATC CTGGGCGTGG TCTTGGGTTT GCTGATGGTA 
TTCTGGTATC CCCATCAAAT GATCTGGCAG AAGAGCCTGA TGCACAAGAT CGAGATTAGT 
TCCATCGAGA CGAATAGCTC GTTGGAGCCT GTGAAGAATA ATGATGTGGA GGACTCGCCA 
CTGCTGAAGG GATTGCAATA TGCAGCGGCC AAAGATGGGG CGGGAAATGG AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG7227 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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