LKRSDH cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LKRSDH
Entrez Gene ID	34064
Full Name	Lysine ketoglutarate reductase/saccharopine dehydrogenase
Synonyms	BEST:CK02318,CG7144,CK02318,Dmel\CG7144,LKR,SDH,anon-WO0149856.1,dLKR
General protein information	Preferred Names Lysine ketoglutarate reductase/saccharopine dehydrogenase Names CG7144 gene product from transcript CG7144-RB CG7144-PA CG7144-PB LKRSDH-PA LKRSDH-PB drosophila lysine ketoglutarate reductase drosophila lysine ketoglutarate reductase (dLKR)/saccharopine dehydrogenase (SDH) lysine ketoglutarate reductase lysine ketoglutarate reductase/saccharopine dehydrogenase saccharopine dehydogenase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 28C4-28C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_135306.3	NP_609150.2	lysine ketoglutarate reductase/saccharopine dehydrogenase, isoform A
NM_001298790.1	NP_001285719.1	lysine ketoglutarate reductase/saccharopine dehydrogenase, isoform B

Pathways from BioSystems

Homologies

	AASS	XP_002803495.1
Canis lupus familiaris (dog)	AASS	XP_005628825.1
Bos taurus (cattle)	AASS	NP_001103267.1
Rattus norvegicus (Norway rat)	Aass	NP_001094433.1
Magnaporthe oryzae (rice blast fungus)	MGG_17041	XP_003716085.1
Arabidopsis thaliana (thale cress)	AT4G33150	NP_567914.1
Homo sapiens (human)	AASS	NP_005754.2
Gallus gallus (chicken)	AASS	XP_416001.2
Schizosaccharomyces pombe (fission yeast)	SPBC3B8.03	NP_596411.1
Oryza sativa (rice)	Os02g0783700	NP_001048324.1
Pan troglodytes (chimpanzee)	AASS	XP_001145257.1
Caenorhabditis elegans (roundworm)	CELE_R02D3.1	NP_499884.1
Neurospora crassa	NCU03748	XP_961095.2
Xenopus tropicalis (tropical clawed frog)	aass	NP_001011437.1
Mus musculus (house mouse)	Aass	NP_038958.2
Danio rerio (zebrafish)	aass	NP_001167456.1
Drosophila melanogaster (fruit fly)	LKR	NP_609150.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008632	XP_314728.3
Saccharomyces cerevisiae (baker's yeast)	LYS9	NP_014448.1
Kluyveromyces lactis	KLLA0C18744g	XP_453035.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel cofactor, Drosophila lysine ketoglutarate reductase (dLKR)/saccharopine dehydrogenase (SDH), is involved in ecdysone-mediated transcription.
Title: dLKR/SDH regulates hormone-mediated histone arginine methylation and transcription of cell death genes.

The following LKRSDH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LKRSDH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16403	NM_135306.3 Latest version!	Drosophila melanogaster lysine ketoglutarate reductase/saccharopine dehydrogenase, transcript variant A (LKRSDH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16403	NM_001298790.1 Latest version!	Drosophila melanogaster lysine ketoglutarate reductase/saccharopine dehydrogenase, transcript variant B (LKRSDH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LKRSDH ( NM_135306.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609150.2 Drosophila melanogaster lysine ketoglutarate reductase/saccharopine dehydrogenase, transcript variant A (LKRSDH), mRNA.

Price & Availability↑

CloneID	OFa16403
Clone ID Related Accession (Same CDS sequence)	NM_135306.3 , NM_001298790.1
Accession Version	NM_135306.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lysine ketoglutarate reductase/saccharopine dehydrogenase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135306.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGCGAG TGATTCAACT GCGCGCAACA ATCGCGCATC CGTTCACCAG ACAACGTCAT 
AGTCGAGTGA TTGCCATTCG GCGAGAGGAT CAGTCGGTGT GGGAGCGACG AGCTCCGTTT 
GGACCAACCC ACGTCCAGAA GTTGGTCAAG CAGAATGTCA AGGTCATAGT GCAGCCGTCC 
AATCGTCGTG CCTACCCCAT GCAGGCCTAC ATGCAGGCTG GAGCTCATAT TCAGGAGGAC 
ATCAGTGATG CTTCGGTGAT ATTCGGAGTG AAACAGGTGC CCATAGATGC ACTGATTCCT 
GGGAAGACCT ACTGCTTCTT TTCGCACACG ATTAAAGCGC AGGAATCAAA TATGCCGCTT 
TTGGATGCTA TTTTGGAGAA GAAAATTCGG CTCATCGACT ATGAGCGAAT CATCGACGAA 
CGCGGAGCAC GACAGGTGGC CTTTGGCAAA TATGCCGGAG TGGCTGGCAT GGTGAACATC 
CTGCACGGCA TTGGACTGCG TCTTTTGGCC CTGGGACATC ATACACCATT CATGCACATT 
GGACCCGCTC ATAACTATCG CAATTCCTCG ATGGCTCGCC AGGCAATCCG GGATTGTGGC 
TACGAGATCT CGCTTGGCAT GATGCCCAAG TCCATTGGAC CACTTACATT TGTGTTCACT 
GGCTCTGGAA ATGTTTCCCA AGGTGCCCAG GAGGTGTTCT CCGAGCTGCC CATTGAGTAT 
GTTCCACCGG AGATGCTACG CAAGGTGGCC GAACATGGAA ATCAAAATAA GCTTTACGGC 
TGCGAGGTGA GCCGATCGGA TCATCTGGAG CGTCGTGAGG GCGGCGGATT TGATGCCAAG 
GAATACGATG AGTTCCCCGA ACGATACATC TCCACCTTTA GCACGAAGAT AGCTCCGTAC 
GCATCGGTTA TTGTTAACGG CATCTACTGG GCCGTAGGCA GTCCCAAGTT GATCAGCATT 
CCGGATGCCA AGAATCTGCT ACGTCCGGCA AACACTCCCT GGCTGCCGAC GAGCAAGGGT 
AGTCCGGCTT TGCCACATCG CATGCTGGCC ATTTGCGACA TTTCCGCTGA TCCCGGTGGC 
TCCATTGAGT TTATGAACGA GTGCACCACC ATCGACACTC CATTCTGCTT GTACGATGCG 
GACAGGAATA AGGATACCAA GAGCTTTAAG GGACCCGGAG TTTTAGTCTG TTCGATTGAT 
AATATGCCTA CACAATTGCC CAGAGAATCG ACGGATTTGT TTGGAGAGCT GTTGGCTCCT 
CATGTTCATG ATATTATAAA GAGCGATGCC AAGAAGCCGT TGGCAGAGGA GAACTTCTCA 
TACCCCATTC AGTCGGCTAT TATCGCCAGC AACGGTCAGC TGACGGAGGG CTTCCAGTAT 
ATTCAGGAGC TCCGAGAGTC GCAAAGCCAC AGATCGCGCC ACAAGATGGA GGGAAGTTCG 
GAGTCTGACA AGAAGGTCTT GGTTCTGGGA GCTGGCATGG TGTCCGCTCC ACTCGTAGAG 
TGGCTTCATC GCGAAAAGGA CGTCAGCATA ACGGTTTGCT CGCAGGTCAA GGAGGAAGCC 
GATCGCCTGG CTCAGCAGTA TGCTGGAGTG GACAGCGTCT ATCTGGATGT GAACGAGAGC 
ACTGGTCATC TGCAGGAGCT GTGCGGCAGG GCAGATGTAG TAGTTTCCCT GCTGCCCTAC 
AGTCTCCATG GCATGGTGGC ACGTTATTGC GTGGCAGAGG GCACCCACAT GGTTACCGCA 
AGTTATCTGA ACGACGAGAT CTCCGGTTTG CACGAGGAGG CCAAGGCCAA GGGTGTGACC 
ATCATGAACG AGGTGGGCTT GGATCCTGGT ATCGATCACC TTCTGGCACT GGAGTGCATC 
CACGAGGTGC AGGACAAGGG AGCAGTTGTC GAGTCCTTCG TGAGCTATTG TGGCGGTCTG 
CCTGCACCGG AGCATTCCAA TAATGCCCTA AGATATAAGT TCTCTTGGTC ACCCAGGGGA 
GTACTTCTCA ACACACTTTC CGCTGCAAAA TATCTGAGTC AGGGACAAAT TGTGGAGATT 
TCTGGAGGTG GGGAACTCAT GTCAAGTCCT CGCAGCTTGG ATTTCCTGCC AGGATTCGCC 
CTGGAGGGTT TCCCCAACAG GGATTCCACC AAATACGGCA ACCTTTACGG CTTAGGCAGG 
GATGTGCACA CTTTGCTTCG TGGAACCATA CGTTATAAGG GCTTTTCGGA ATCGATTAAG 
CCTATGCAGC TCTTGGGACT TATCGATCCG GAGCCACATG CATTGCTACA TCCCAGTGGG 
CCGGATGTCA CGTGGCGCCA GCTGGTGATC CATCTAATGG GCATGTCGGA CTCGACCATC 
TTCTACGAGA ACCTCAAGCA GAAGCTGACT GAGCGCATTG GAGATGTGGA CGGCATCGAG 
AGCTTGGGTC TGCTGGATGA TACTCCTGTG GTGAAGCTCA ACACTCCGCT GGATACGCTT 
AGTCACTATC TCTCGAAGAG ACTTGCCTTC GAGCGGGATG AACGCGACCT GGTGGTGCTG 
CGCCATGAAG TTGGCATCCG TTGGCCCGAT GGTCGTCGTG AGGAGCGTGG AATCAATTTT 
GTGGTCTACG GACAGCCCCA GGGTCACTCC GCCATGGCGA TGACTGTGGG CAAGCCGGCT 
GCCATCGCAG CCAAAATGAT CCTAGACGGT GAGATCCAGG AACGCGGCGT CCTGCTGCCC 
TTCACTCCCG ACATTTATCG TCCCATGCTG CAACGTCTGC GCTCCGAGGG TCTGACTGCC 
ACGGAAACCT CCAGATGGCT AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609150.2
CDS	126..2912
Translation	MWRVIQLRATIAHPFTRQRHSRVIAIRREDQSVWERRAPFGPTHVQKLVKQNVKVIVQPSNRRAYPMQAYMQAGAHIQEDISDASVIFGVKQVPIDALIPGKTYCFFSHTIKAQESNMPLLDAILEKKIRLIDYERIIDERGARQVAFGKYAGVAGMVNILHGIGLRLLALGHHTPFMHIGPAHNYRNSSMARQAIRDCGYEISLGMMPKSIGPLTFVFTGSGNVSQGAQEVFSELPIEYVPPEMLRKVAEHGNQNKLYGCEVSRSDHLERREGGGFDAKEYDEFPERYISTFSTKIAPYASVIVNGIYWAVGSPKLISIPDAKNLLRPANTPWLPTSKGSPALPHRMLAICDISADPGGSIEFMNECTTIDTPFCLYDADRNKDTKSFKGPGVLVCSIDNMPTQLPRESTDLFGELLAPHVHDIIKSDAKKPLAEENFSYPIQSAIIASNGQLTEGFQYIQELRESQSHRSRHKMEGSSESDKKVLVLGAGMVSAPLVEWLHREKDVSITVCSQVKEEADRLAQQYAGVDSVYLDVNESTGHLQELCGRADVVVSLLPYSLHGMVARYCVAEGTHMVTASYLNDEISGLHEEAKAKGVTIMNEVGLDPGIDHLLALECIHEVQDKGAVVESFVSYCGGLPAPEHSNNALRYKFSWSPRGVLLNTLSAAKYLSQGQIVEISGGGELMSSPRSLDFLPGFALEGFPNRDSTKYGNLYGLGRDVHTLLRGTIRYKGFSESIKPMQLLGLIDPEPHALLHPSGPDVTWRQLVIHLMGMSDSTIFYENLKQKLTERIGDVDGIESLGLLDDTPVVKLNTPLDTLSHYLSKRLAFERDERDLVVLRHEVGIRWPDGRREERGINFVVYGQPQGHSAMAMTVGKPAAIAAKMILDGEIQERGVLLPFTPDIYRPMLQRLRSEGLTATETSRWLN

Target ORF information:

RefSeq Version	NM_135306.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lysine ketoglutarate reductase/saccharopine dehydrogenase, transcript variant A (LKRSDH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135306.3

ORF Insert Sequence:

ATGTGGCGAG TGATTCAACT GCGCGCAACA ATCGCGCATC CGTTCACCAG ACAACGTCAT 
AGTCGAGTGA TTGCCATTCG GCGAGAGGAT CAGTCGGTGT GGGAGCGACG AGCTCCGTTT 
GGACCAACCC ACGTCCAGAA GTTGGTCAAG CAGAATGTCA AGGTCATAGT GCAGCCGTCC 
AATCGTCGTG CCTACCCCAT GCAGGCCTAC ATGCAGGCTG GAGCTCATAT TCAGGAGGAC 
ATCAGTGATG CTTCGGTGAT ATTCGGAGTG AAACAGGTGC CCATAGATGC ACTGATTCCT 
GGGAAGACCT ACTGCTTCTT TTCGCACACG ATTAAAGCGC AGGAATCAAA TATGCCGCTT 
TTGGATGCTA TTTTGGAGAA GAAAATTCGG CTCATCGACT ATGAGCGAAT CATCGACGAA 
CGCGGAGCAC GACAGGTGGC CTTTGGCAAA TATGCCGGAG TGGCTGGCAT GGTGAACATC 
CTGCACGGCA TTGGACTGCG TCTTTTGGCC CTGGGACATC ATACACCATT CATGCACATT 
GGACCCGCTC ATAACTATCG CAATTCCTCG ATGGCTCGCC AGGCAATCCG GGATTGTGGC 
TACGAGATCT CGCTTGGCAT GATGCCCAAG TCCATTGGAC CACTTACATT TGTGTTCACT 
GGCTCTGGAA ATGTTTCCCA AGGTGCCCAG GAGGTGTTCT CCGAGCTGCC CATTGAGTAT 
GTTCCACCGG AGATGCTACG CAAGGTGGCC GAACATGGAA ATCAAAATAA GCTTTACGGC 
TGCGAGGTGA GCCGATCGGA TCATCTGGAG CGTCGTGAGG GCGGCGGATT TGATGCCAAG 
GAATACGATG AGTTCCCCGA ACGATACATC TCCACCTTTA GCACGAAGAT AGCTCCGTAC 
GCATCGGTTA TTGTTAACGG CATCTACTGG GCCGTAGGCA GTCCCAAGTT GATCAGCATT 
CCGGATGCCA AGAATCTGCT ACGTCCGGCA AACACTCCCT GGCTGCCGAC GAGCAAGGGT 
AGTCCGGCTT TGCCACATCG CATGCTGGCC ATTTGCGACA TTTCCGCTGA TCCCGGTGGC 
TCCATTGAGT TTATGAACGA GTGCACCACC ATCGACACTC CATTCTGCTT GTACGATGCG 
GACAGGAATA AGGATACCAA GAGCTTTAAG GGACCCGGAG TTTTAGTCTG TTCGATTGAT 
AATATGCCTA CACAATTGCC CAGAGAATCG ACGGATTTGT TTGGAGAGCT GTTGGCTCCT 
CATGTTCATG ATATTATAAA GAGCGATGCC AAGAAGCCGT TGGCAGAGGA GAACTTCTCA 
TACCCCATTC AGTCGGCTAT TATCGCCAGC AACGGTCAGC TGACGGAGGG CTTCCAGTAT 
ATTCAGGAGC TCCGAGAGTC GCAAAGCCAC AGATCGCGCC ACAAGATGGA GGGAAGTTCG 
GAGTCTGACA AGAAGGTCTT GGTTCTGGGA GCTGGCATGG TGTCCGCTCC ACTCGTAGAG 
TGGCTTCATC GCGAAAAGGA CGTCAGCATA ACGGTTTGCT CGCAGGTCAA GGAGGAAGCC 
GATCGCCTGG CTCAGCAGTA TGCTGGAGTG GACAGCGTCT ATCTGGATGT GAACGAGAGC 
ACTGGTCATC TGCAGGAGCT GTGCGGCAGG GCAGATGTAG TAGTTTCCCT GCTGCCCTAC 
AGTCTCCATG GCATGGTGGC ACGTTATTGC GTGGCAGAGG GCACCCACAT GGTTACCGCA 
AGTTATCTGA ACGACGAGAT CTCCGGTTTG CACGAGGAGG CCAAGGCCAA GGGTGTGACC 
ATCATGAACG AGGTGGGCTT GGATCCTGGT ATCGATCACC TTCTGGCACT GGAGTGCATC 
CACGAGGTGC AGGACAAGGG AGCAGTTGTC GAGTCCTTCG TGAGCTATTG TGGCGGTCTG 
CCTGCACCGG AGCATTCCAA TAATGCCCTA AGATATAAGT TCTCTTGGTC ACCCAGGGGA 
GTACTTCTCA ACACACTTTC CGCTGCAAAA TATCTGAGTC AGGGACAAAT TGTGGAGATT 
TCTGGAGGTG GGGAACTCAT GTCAAGTCCT CGCAGCTTGG ATTTCCTGCC AGGATTCGCC 
CTGGAGGGTT TCCCCAACAG GGATTCCACC AAATACGGCA ACCTTTACGG CTTAGGCAGG 
GATGTGCACA CTTTGCTTCG TGGAACCATA CGTTATAAGG GCTTTTCGGA ATCGATTAAG 
CCTATGCAGC TCTTGGGACT TATCGATCCG GAGCCACATG CATTGCTACA TCCCAGTGGG 
CCGGATGTCA CGTGGCGCCA GCTGGTGATC CATCTAATGG GCATGTCGGA CTCGACCATC 
TTCTACGAGA ACCTCAAGCA GAAGCTGACT GAGCGCATTG GAGATGTGGA CGGCATCGAG 
AGCTTGGGTC TGCTGGATGA TACTCCTGTG GTGAAGCTCA ACACTCCGCT GGATACGCTT 
AGTCACTATC TCTCGAAGAG ACTTGCCTTC GAGCGGGATG AACGCGACCT GGTGGTGCTG 
CGCCATGAAG TTGGCATCCG TTGGCCCGAT GGTCGTCGTG AGGAGCGTGG AATCAATTTT 
GTGGTCTACG GACAGCCCCA GGGTCACTCC GCCATGGCGA TGACTGTGGG CAAGCCGGCT 
GCCATCGCAG CCAAAATGAT CCTAGACGGT GAGATCCAGG AACGCGGCGT CCTGCTGCCC 
TTCACTCCCG ACATTTATCG TCCCATGCTG CAACGTCTGC GCTCCGAGGG TCTGACTGCC 
ACGGAAACCT CCAGATGGCT AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LKRSDH ( NM_001298790.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285719.1 Drosophila melanogaster lysine ketoglutarate reductase/saccharopine dehydrogenase, transcript variant B (LKRSDH), mRNA.

Price & Availability↑

CloneID	OFa16403
Clone ID Related Accession (Same CDS sequence)	NM_135306.3 , NM_001298790.1
Accession Version	NM_001298790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lysine ketoglutarate reductase/saccharopine dehydrogenase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGCGAG TGATTCAACT GCGCGCAACA ATCGCGCATC CGTTCACCAG ACAACGTCAT 
AGTCGAGTGA TTGCCATTCG GCGAGAGGAT CAGTCGGTGT GGGAGCGACG AGCTCCGTTT 
GGACCAACCC ACGTCCAGAA GTTGGTCAAG CAGAATGTCA AGGTCATAGT GCAGCCGTCC 
AATCGTCGTG CCTACCCCAT GCAGGCCTAC ATGCAGGCTG GAGCTCATAT TCAGGAGGAC 
ATCAGTGATG CTTCGGTGAT ATTCGGAGTG AAACAGGTGC CCATAGATGC ACTGATTCCT 
GGGAAGACCT ACTGCTTCTT TTCGCACACG ATTAAAGCGC AGGAATCAAA TATGCCGCTT 
TTGGATGCTA TTTTGGAGAA GAAAATTCGG CTCATCGACT ATGAGCGAAT CATCGACGAA 
CGCGGAGCAC GACAGGTGGC CTTTGGCAAA TATGCCGGAG TGGCTGGCAT GGTGAACATC 
CTGCACGGCA TTGGACTGCG TCTTTTGGCC CTGGGACATC ATACACCATT CATGCACATT 
GGACCCGCTC ATAACTATCG CAATTCCTCG ATGGCTCGCC AGGCAATCCG GGATTGTGGC 
TACGAGATCT CGCTTGGCAT GATGCCCAAG TCCATTGGAC CACTTACATT TGTGTTCACT 
GGCTCTGGAA ATGTTTCCCA AGGTGCCCAG GAGGTGTTCT CCGAGCTGCC CATTGAGTAT 
GTTCCACCGG AGATGCTACG CAAGGTGGCC GAACATGGAA ATCAAAATAA GCTTTACGGC 
TGCGAGGTGA GCCGATCGGA TCATCTGGAG CGTCGTGAGG GCGGCGGATT TGATGCCAAG 
GAATACGATG AGTTCCCCGA ACGATACATC TCCACCTTTA GCACGAAGAT AGCTCCGTAC 
GCATCGGTTA TTGTTAACGG CATCTACTGG GCCGTAGGCA GTCCCAAGTT GATCAGCATT 
CCGGATGCCA AGAATCTGCT ACGTCCGGCA AACACTCCCT GGCTGCCGAC GAGCAAGGGT 
AGTCCGGCTT TGCCACATCG CATGCTGGCC ATTTGCGACA TTTCCGCTGA TCCCGGTGGC 
TCCATTGAGT TTATGAACGA GTGCACCACC ATCGACACTC CATTCTGCTT GTACGATGCG 
GACAGGAATA AGGATACCAA GAGCTTTAAG GGACCCGGAG TTTTAGTCTG TTCGATTGAT 
AATATGCCTA CACAATTGCC CAGAGAATCG ACGGATTTGT TTGGAGAGCT GTTGGCTCCT 
CATGTTCATG ATATTATAAA GAGCGATGCC AAGAAGCCGT TGGCAGAGGA GAACTTCTCA 
TACCCCATTC AGTCGGCTAT TATCGCCAGC AACGGTCAGC TGACGGAGGG CTTCCAGTAT 
ATTCAGGAGC TCCGAGAGTC GCAAAGCCAC AGATCGCGCC ACAAGATGGA GGGAAGTTCG 
GAGTCTGACA AGAAGGTCTT GGTTCTGGGA GCTGGCATGG TGTCCGCTCC ACTCGTAGAG 
TGGCTTCATC GCGAAAAGGA CGTCAGCATA ACGGTTTGCT CGCAGGTCAA GGAGGAAGCC 
GATCGCCTGG CTCAGCAGTA TGCTGGAGTG GACAGCGTCT ATCTGGATGT GAACGAGAGC 
ACTGGTCATC TGCAGGAGCT GTGCGGCAGG GCAGATGTAG TAGTTTCCCT GCTGCCCTAC 
AGTCTCCATG GCATGGTGGC ACGTTATTGC GTGGCAGAGG GCACCCACAT GGTTACCGCA 
AGTTATCTGA ACGACGAGAT CTCCGGTTTG CACGAGGAGG CCAAGGCCAA GGGTGTGACC 
ATCATGAACG AGGTGGGCTT GGATCCTGGT ATCGATCACC TTCTGGCACT GGAGTGCATC 
CACGAGGTGC AGGACAAGGG AGCAGTTGTC GAGTCCTTCG TGAGCTATTG TGGCGGTCTG 
CCTGCACCGG AGCATTCCAA TAATGCCCTA AGATATAAGT TCTCTTGGTC ACCCAGGGGA 
GTACTTCTCA ACACACTTTC CGCTGCAAAA TATCTGAGTC AGGGACAAAT TGTGGAGATT 
TCTGGAGGTG GGGAACTCAT GTCAAGTCCT CGCAGCTTGG ATTTCCTGCC AGGATTCGCC 
CTGGAGGGTT TCCCCAACAG GGATTCCACC AAATACGGCA ACCTTTACGG CTTAGGCAGG 
GATGTGCACA CTTTGCTTCG TGGAACCATA CGTTATAAGG GCTTTTCGGA ATCGATTAAG 
CCTATGCAGC TCTTGGGACT TATCGATCCG GAGCCACATG CATTGCTACA TCCCAGTGGG 
CCGGATGTCA CGTGGCGCCA GCTGGTGATC CATCTAATGG GCATGTCGGA CTCGACCATC 
TTCTACGAGA ACCTCAAGCA GAAGCTGACT GAGCGCATTG GAGATGTGGA CGGCATCGAG 
AGCTTGGGTC TGCTGGATGA TACTCCTGTG GTGAAGCTCA ACACTCCGCT GGATACGCTT 
AGTCACTATC TCTCGAAGAG ACTTGCCTTC GAGCGGGATG AACGCGACCT GGTGGTGCTG 
CGCCATGAAG TTGGCATCCG TTGGCCCGAT GGTCGTCGTG AGGAGCGTGG AATCAATTTT 
GTGGTCTACG GACAGCCCCA GGGTCACTCC GCCATGGCGA TGACTGTGGG CAAGCCGGCT 
GCCATCGCAG CCAAAATGAT CCTAGACGGT GAGATCCAGG AACGCGGCGT CCTGCTGCCC 
TTCACTCCCG ACATTTATCG TCCCATGCTG CAACGTCTGC GCTCCGAGGG TCTGACTGCC 
ACGGAAACCT CCAGATGGCT AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285719.1
CDS	126..2912
Translation	MWRVIQLRATIAHPFTRQRHSRVIAIRREDQSVWERRAPFGPTHVQKLVKQNVKVIVQPSNRRAYPMQAYMQAGAHIQEDISDASVIFGVKQVPIDALIPGKTYCFFSHTIKAQESNMPLLDAILEKKIRLIDYERIIDERGARQVAFGKYAGVAGMVNILHGIGLRLLALGHHTPFMHIGPAHNYRNSSMARQAIRDCGYEISLGMMPKSIGPLTFVFTGSGNVSQGAQEVFSELPIEYVPPEMLRKVAEHGNQNKLYGCEVSRSDHLERREGGGFDAKEYDEFPERYISTFSTKIAPYASVIVNGIYWAVGSPKLISIPDAKNLLRPANTPWLPTSKGSPALPHRMLAICDISADPGGSIEFMNECTTIDTPFCLYDADRNKDTKSFKGPGVLVCSIDNMPTQLPRESTDLFGELLAPHVHDIIKSDAKKPLAEENFSYPIQSAIIASNGQLTEGFQYIQELRESQSHRSRHKMEGSSESDKKVLVLGAGMVSAPLVEWLHREKDVSITVCSQVKEEADRLAQQYAGVDSVYLDVNESTGHLQELCGRADVVVSLLPYSLHGMVARYCVAEGTHMVTASYLNDEISGLHEEAKAKGVTIMNEVGLDPGIDHLLALECIHEVQDKGAVVESFVSYCGGLPAPEHSNNALRYKFSWSPRGVLLNTLSAAKYLSQGQIVEISGGGELMSSPRSLDFLPGFALEGFPNRDSTKYGNLYGLGRDVHTLLRGTIRYKGFSESIKPMQLLGLIDPEPHALLHPSGPDVTWRQLVIHLMGMSDSTIFYENLKQKLTERIGDVDGIESLGLLDDTPVVKLNTPLDTLSHYLSKRLAFERDERDLVVLRHEVGIRWPDGRREERGINFVVYGQPQGHSAMAMTVGKPAAIAAKMILDGEIQERGVLLPFTPDIYRPMLQRLRSEGLTATETSRWLN

Target ORF information:

RefSeq Version	NM_001298790.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lysine ketoglutarate reductase/saccharopine dehydrogenase, transcript variant B (LKRSDH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298790.1

ORF Insert Sequence:

ATGTGGCGAG TGATTCAACT GCGCGCAACA ATCGCGCATC CGTTCACCAG ACAACGTCAT 
AGTCGAGTGA TTGCCATTCG GCGAGAGGAT CAGTCGGTGT GGGAGCGACG AGCTCCGTTT 
GGACCAACCC ACGTCCAGAA GTTGGTCAAG CAGAATGTCA AGGTCATAGT GCAGCCGTCC 
AATCGTCGTG CCTACCCCAT GCAGGCCTAC ATGCAGGCTG GAGCTCATAT TCAGGAGGAC 
ATCAGTGATG CTTCGGTGAT ATTCGGAGTG AAACAGGTGC CCATAGATGC ACTGATTCCT 
GGGAAGACCT ACTGCTTCTT TTCGCACACG ATTAAAGCGC AGGAATCAAA TATGCCGCTT 
TTGGATGCTA TTTTGGAGAA GAAAATTCGG CTCATCGACT ATGAGCGAAT CATCGACGAA 
CGCGGAGCAC GACAGGTGGC CTTTGGCAAA TATGCCGGAG TGGCTGGCAT GGTGAACATC 
CTGCACGGCA TTGGACTGCG TCTTTTGGCC CTGGGACATC ATACACCATT CATGCACATT 
GGACCCGCTC ATAACTATCG CAATTCCTCG ATGGCTCGCC AGGCAATCCG GGATTGTGGC 
TACGAGATCT CGCTTGGCAT GATGCCCAAG TCCATTGGAC CACTTACATT TGTGTTCACT 
GGCTCTGGAA ATGTTTCCCA AGGTGCCCAG GAGGTGTTCT CCGAGCTGCC CATTGAGTAT 
GTTCCACCGG AGATGCTACG CAAGGTGGCC GAACATGGAA ATCAAAATAA GCTTTACGGC 
TGCGAGGTGA GCCGATCGGA TCATCTGGAG CGTCGTGAGG GCGGCGGATT TGATGCCAAG 
GAATACGATG AGTTCCCCGA ACGATACATC TCCACCTTTA GCACGAAGAT AGCTCCGTAC 
GCATCGGTTA TTGTTAACGG CATCTACTGG GCCGTAGGCA GTCCCAAGTT GATCAGCATT 
CCGGATGCCA AGAATCTGCT ACGTCCGGCA AACACTCCCT GGCTGCCGAC GAGCAAGGGT 
AGTCCGGCTT TGCCACATCG CATGCTGGCC ATTTGCGACA TTTCCGCTGA TCCCGGTGGC 
TCCATTGAGT TTATGAACGA GTGCACCACC ATCGACACTC CATTCTGCTT GTACGATGCG 
GACAGGAATA AGGATACCAA GAGCTTTAAG GGACCCGGAG TTTTAGTCTG TTCGATTGAT 
AATATGCCTA CACAATTGCC CAGAGAATCG ACGGATTTGT TTGGAGAGCT GTTGGCTCCT 
CATGTTCATG ATATTATAAA GAGCGATGCC AAGAAGCCGT TGGCAGAGGA GAACTTCTCA 
TACCCCATTC AGTCGGCTAT TATCGCCAGC AACGGTCAGC TGACGGAGGG CTTCCAGTAT 
ATTCAGGAGC TCCGAGAGTC GCAAAGCCAC AGATCGCGCC ACAAGATGGA GGGAAGTTCG 
GAGTCTGACA AGAAGGTCTT GGTTCTGGGA GCTGGCATGG TGTCCGCTCC ACTCGTAGAG 
TGGCTTCATC GCGAAAAGGA CGTCAGCATA ACGGTTTGCT CGCAGGTCAA GGAGGAAGCC 
GATCGCCTGG CTCAGCAGTA TGCTGGAGTG GACAGCGTCT ATCTGGATGT GAACGAGAGC 
ACTGGTCATC TGCAGGAGCT GTGCGGCAGG GCAGATGTAG TAGTTTCCCT GCTGCCCTAC 
AGTCTCCATG GCATGGTGGC ACGTTATTGC GTGGCAGAGG GCACCCACAT GGTTACCGCA 
AGTTATCTGA ACGACGAGAT CTCCGGTTTG CACGAGGAGG CCAAGGCCAA GGGTGTGACC 
ATCATGAACG AGGTGGGCTT GGATCCTGGT ATCGATCACC TTCTGGCACT GGAGTGCATC 
CACGAGGTGC AGGACAAGGG AGCAGTTGTC GAGTCCTTCG TGAGCTATTG TGGCGGTCTG 
CCTGCACCGG AGCATTCCAA TAATGCCCTA AGATATAAGT TCTCTTGGTC ACCCAGGGGA 
GTACTTCTCA ACACACTTTC CGCTGCAAAA TATCTGAGTC AGGGACAAAT TGTGGAGATT 
TCTGGAGGTG GGGAACTCAT GTCAAGTCCT CGCAGCTTGG ATTTCCTGCC AGGATTCGCC 
CTGGAGGGTT TCCCCAACAG GGATTCCACC AAATACGGCA ACCTTTACGG CTTAGGCAGG 
GATGTGCACA CTTTGCTTCG TGGAACCATA CGTTATAAGG GCTTTTCGGA ATCGATTAAG 
CCTATGCAGC TCTTGGGACT TATCGATCCG GAGCCACATG CATTGCTACA TCCCAGTGGG 
CCGGATGTCA CGTGGCGCCA GCTGGTGATC CATCTAATGG GCATGTCGGA CTCGACCATC 
TTCTACGAGA ACCTCAAGCA GAAGCTGACT GAGCGCATTG GAGATGTGGA CGGCATCGAG 
AGCTTGGGTC TGCTGGATGA TACTCCTGTG GTGAAGCTCA ACACTCCGCT GGATACGCTT 
AGTCACTATC TCTCGAAGAG ACTTGCCTTC GAGCGGGATG AACGCGACCT GGTGGTGCTG 
CGCCATGAAG TTGGCATCCG TTGGCCCGAT GGTCGTCGTG AGGAGCGTGG AATCAATTTT 
GTGGTCTACG GACAGCCCCA GGGTCACTCC GCCATGGCGA TGACTGTGGG CAAGCCGGCT 
GCCATCGCAG CCAAAATGAT CCTAGACGGT GAGATCCAGG AACGCGGCGT CCTGCTGCCC 
TTCACTCCCG ACATTTATCG TCCCATGCTG CAACGTCTGC GCTCCGAGGG TCTGACTGCC 
ACGGAAACCT CCAGATGGCT AAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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LKRSDH cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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