CG5149 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG5149
Entrez Gene ID	34013
Full Name	CG5149 gene product from transcript CG5149-RA
Synonyms	Dmel\CG5149
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 27F3-27F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_135266.4	NP_609110.1	uncharacterized protein

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007765	XP_317751.3
Drosophila melanogaster (fruit fly)	CG5149	NP_609110.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG5149 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG5149 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14022	NM_135266.4 Latest version!	Drosophila melanogaster uncharacterized protein (CG5149), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG5149 ( NM_135266.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609110.1 Drosophila melanogaster uncharacterized protein (CG5149), mRNA.

Price & Availability↑

CloneID	OFa14022
Clone ID Related Accession (Same CDS sequence)	NM_135266.4
Accession Version	NM_135266.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135266.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAATG CGAGTGGCAA GCGGGAGACG GATAATCTCT ACAATTCGGA AGAGCTACGC 
ATGCTGGAAT CCGCGTACAA AAACGCCTCT GGCGGAGCAC TTGAGAAGTT GACCCAAGAC 
AGATTGGTGG AAACTTGGTC GCAAACCATC GAAAGATCCC TGGCCGAAAG TACGGCTCAG 
TATCTATTTA CGCCCACGAA GCCAGGTCAG CAATGTGTCA ACCTTCAGCT GAAGAAATTT 
GGAGAGCCCT ACTACATCAT GGAACGCGGT ACTATTGACC AGAAAATGCA AATGCTCCTG 
GGCTCAATGG AGAGAAGTGG AAATGACACC TTCAACAGCA AGCAATTGGA GCAATATATC 
TACTCGGTGA TCAAGAGCTA CGTGCACTTG GAAAGTACAG CGAAAAACTC TGGCATCAAG 
GAGTGGCACG ATCTTGGCTT TAACACCACG GAAAGGTCCG CCGCCACATT TGCCAAGGGC 
CTTATGAGAA ATCTGGGCAA GGAGCTAGAG CACACTATGC CCAACGATGC CCTGGAACGT 
TGGTTACACG TCACTCCGCA GTTTTTGCAG ATTTGGCGCG AGGTCTTTAG CCAGCTGTAT 
TGTCGCCATG GTGGCAGCAA ACGCAACATA ATCAAGGAAA TGGAAATTCC AATTCTGCCA 
GCACTGTGTG ATGCTCCGCA AAATAGTCAT TATCGCCCTA TCATCGAACT ACCTCACGTC 
CTGTATATCA ATGCTCAGCT GCCCCGCGAA CATCGGCACA AATGGCGCTT TCTATTCTCA 
TCGAAAATCA ACGGGGAAAG CTTCTCGACG ATGTTGGGAA AAGTGCTGGA CAAAGGACCA 
ACGCTGTTCT TTATTGAGGA CGAGGATCAA TATATATTTG GCGGCTACGC GTCCGAAACG 
TGGTCCGTGA AACCGCAATT TGGAGGCGAT GACAGCTCAT TGCTCTACAC CTTAAGCCCT 
GCCATGCGGT GCTTTTCGGC CACAACTTAT AACAATCACT ATCAGTATTT GAATTTGAAT 
CAGCAGACTA TGCCGAATGG CCTGGGCATG GGCGGTCAGT TTGATTTTTG GGGCCTTTGG 
ATTGACTGCA GTTTCGGCGA TGGACAAAGC GTTGAAAGTT GCACTACATA TCGAGACTAC 
GTGCAGTTGA GTAAACGGAA ACAGTTCAAA ATACGAAACA TGGAAGTTTG GGCCGTAGGA 
GACTTACCAG TTAAAGAAGA AGACGAAGAA GGCGACGGTC ACAAACGTTC TGTGCTGGAT 
GGCAATTTGG AAGATCGGGC CATGTTGGAA ATTGCGGGCA AGAAAATGCA CTCGGATGGA 
TTGCGTGAGC CCAACATGGA TGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609110.1
CDS	151..1497
Translation	MGNASGKRETDNLYNSEELRMLESAYKNASGGALEKLTQDRLVETWSQTIERSLAESTAQYLFTPTKPGQQCVNLQLKKFGEPYYIMERGTIDQKMQMLLGSMERSGNDTFNSKQLEQYIYSVIKSYVHLESTAKNSGIKEWHDLGFNTTERSAATFAKGLMRNLGKELEHTMPNDALERWLHVTPQFLQIWREVFSQLYCRHGGSKRNIIKEMEIPILPALCDAPQNSHYRPIIELPHVLYINAQLPREHRHKWRFLFSSKINGESFSTMLGKVLDKGPTLFFIEDEDQYIFGGYASETWSVKPQFGGDDSSLLYTLSPAMRCFSATTYNNHYQYLNLNQQTMPNGLGMGGQFDFWGLWIDCSFGDGQSVESCTTYRDYVQLSKRKQFKIRNMEVWAVGDLPVKEEDEEGDGHKRSVLDGNLEDRAMLEIAGKKMHSDGLREPNMDD

Target ORF information:

RefSeq Version	NM_135266.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG5149), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135266.4

ORF Insert Sequence:

ATGGGAAATG CGAGTGGCAA GCGGGAGACG GATAATCTCT ACAATTCGGA AGAGCTACGC 
ATGCTGGAAT CCGCGTACAA AAACGCCTCT GGCGGAGCAC TTGAGAAGTT GACCCAAGAC 
AGATTGGTGG AAACTTGGTC GCAAACCATC GAAAGATCCC TGGCCGAAAG TACGGCTCAG 
TATCTATTTA CGCCCACGAA GCCAGGTCAG CAATGTGTCA ACCTTCAGCT GAAGAAATTT 
GGAGAGCCCT ACTACATCAT GGAACGCGGT ACTATTGACC AGAAAATGCA AATGCTCCTG 
GGCTCAATGG AGAGAAGTGG AAATGACACC TTCAACAGCA AGCAATTGGA GCAATATATC 
TACTCGGTGA TCAAGAGCTA CGTGCACTTG GAAAGTACAG CGAAAAACTC TGGCATCAAG 
GAGTGGCACG ATCTTGGCTT TAACACCACG GAAAGGTCCG CCGCCACATT TGCCAAGGGC 
CTTATGAGAA ATCTGGGCAA GGAGCTAGAG CACACTATGC CCAACGATGC CCTGGAACGT 
TGGTTACACG TCACTCCGCA GTTTTTGCAG ATTTGGCGCG AGGTCTTTAG CCAGCTGTAT 
TGTCGCCATG GTGGCAGCAA ACGCAACATA ATCAAGGAAA TGGAAATTCC AATTCTGCCA 
GCACTGTGTG ATGCTCCGCA AAATAGTCAT TATCGCCCTA TCATCGAACT ACCTCACGTC 
CTGTATATCA ATGCTCAGCT GCCCCGCGAA CATCGGCACA AATGGCGCTT TCTATTCTCA 
TCGAAAATCA ACGGGGAAAG CTTCTCGACG ATGTTGGGAA AAGTGCTGGA CAAAGGACCA 
ACGCTGTTCT TTATTGAGGA CGAGGATCAA TATATATTTG GCGGCTACGC GTCCGAAACG 
TGGTCCGTGA AACCGCAATT TGGAGGCGAT GACAGCTCAT TGCTCTACAC CTTAAGCCCT 
GCCATGCGGT GCTTTTCGGC CACAACTTAT AACAATCACT ATCAGTATTT GAATTTGAAT 
CAGCAGACTA TGCCGAATGG CCTGGGCATG GGCGGTCAGT TTGATTTTTG GGGCCTTTGG 
ATTGACTGCA GTTTCGGCGA TGGACAAAGC GTTGAAAGTT GCACTACATA TCGAGACTAC 
GTGCAGTTGA GTAAACGGAA ACAGTTCAAA ATACGAAACA TGGAAGTTTG GGCCGTAGGA 
GACTTACCAG TTAAAGAAGA AGACGAAGAA GGCGACGGTC ACAAACGTTC TGTGCTGGAT 
GGCAATTTGG AAGATCGGGC CATGTTGGAA ATTGCGGGCA AGAAAATGCA CTCGGATGGA 
TTGCGTGAGC CCAACATGGA TGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG5149 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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