mEFG1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mEFG1
Entrez Gene ID	34004
Full Name	iconoclast
Synonyms	B,CG4567,Dmel\CG4567,G,GFM1,dEF-G1,l(2)27Cb,l(2)27Ea
General protein information	Preferred Names iconoclast Names CG4567 gene product from transcript CG4567-RA CG4567-PA complementation group B complementation group G ico-PA lethal (2) 27Cb lethal (2) 27Ea
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 27C7-27D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_135261.2	NP_609105.1	mitochondrial translation elongation factor G 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GFM1	NP_079272.4
Bos taurus (cattle)	GFM1	NP_001193078.1
Gallus gallus (chicken)	GFM1	XP_001233639.1
Saccharomyces cerevisiae (baker's yeast)	MEF1	NP_013170.1
Schizosaccharomyces pombe (fission yeast)	SPBC1306.01c	NP_595472.2
Magnaporthe oryzae (rice blast fungus)	MGG_14797	XP_003714541.1
Arabidopsis thaliana (thale cress)	AT2G45030	NP_182029.1
Canis lupus familiaris (dog)	GFM1	XP_534320.2
Kluyveromyces lactis	KLLA0D05467g	XP_453304.1
Arabidopsis thaliana (thale cress)	AT1G45332	NP_175135.1
Mus musculus (house mouse)	Gfm1	NP_613057.2
Rattus norvegicus (Norway rat)	Gfm1	NP_446077.1
Caenorhabditis elegans (roundworm)	CELE_F29C12.4	NP_496787.1
Pan troglodytes (chimpanzee)	GFM1	XP_001154618.1
	GFM1	XP_001094832.2
Danio rerio (zebrafish)	gfm1	NP_001073463.1
Drosophila melanogaster (fruit fly)	ico	NP_609105.1
Anopheles gambiae (African malaria mosquito)	EFGM_ANOGA	XP_318822.4
Eremothecium gossypii	AGOS_ABR227C	NP_983176.2
Neurospora crassa	NCU02955	XP_965342.2
Oryza sativa (rice)	Os03g0565500	NP_001050501.1
Xenopus tropicalis (tropical clawed frog)	gfm1	XP_002933285.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Drosophila mitochondrial translation elongation factor G1 contains a nuclear localization signal and inhibits growth and DPP signaling.
Title: The Drosophila mitochondrial translation elongation factor G1 contains a nuclear localization signal and inhibits growth and DPP signaling.

The following mEFG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mEFG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14011	NM_135261.2 Latest version!	Drosophila melanogaster mitochondrial translation elongation factor G 1 (mEFG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mEFG1 ( NM_135261.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609105.1 Drosophila melanogaster mitochondrial translation elongation factor G 1 (mEFG1), mRNA.

Price & Availability↑

CloneID	OFa14011
Clone ID Related Accession (Same CDS sequence)	NM_135261.2
Accession Version	NM_135261.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial translation elongation factor G 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135261.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCTAA TTACCCGTTT GCTGACCGGG AACAACACCC TACGCCTCCG GGCATTGAAA 
TCACTGGGAA AGGCCGGCTA CAGTTCGCAT GCGAAGTTTT CGGAGCACAA GCCCATCGAG 
AGGATCCGGA ACATAGGAAT CTCGGCGCAT ATTGACAGTG GAAAGACGAC CTTGACGGAG 
AGGATTCTGT TCTACACCGG CAGGATTGCG GAGATGCACG AGGTGCGAGG AAAGGATAAC 
GTGGGTGCCA CCATGGACAG CATGGAGCTG GAAAGGCAGC GGGGCATTAC CATTCAATCG 
GCAGCCACGT ACACACTGTG GAAGGACACC AATATCAATA TTATCGATAC ACCCGGGCAT 
GTGGACTTCA CCGTGGAGGT GGAGCGCGCA CTCCGAGTTC TCGATGGTGC CGTTCTGGTC 
TTGTGCGCCG TGGGAGGAGT CCAGAGTCAG ACCCTGACGG TTAACCGGCA GATGAAGCGC 
TACAATGTGC CCTGCCTGGC TTTCATCAAC AAACTGGATC GCTTGGGGTC GAACCCGTAC 
AGAGTTCTAT CCCAGATGAG GTCAAAAATG AACCACAACG CTGCTTTCAT TCAGCTGCCC 
ATTGGAGTGG AGAGCAATTG CAAGGGCATC GTGGATTTGG TGCGGGAAAA AGCCATATAC 
TTTGAGGGCG AGCACGGAAT GGACATCAGG CTGGACGAAA TTCCACAGGA TATGCGGGTA 
GAGAGCCTGG AGAGAAGACA GGAGCTTATC GAACACTTGT CAAATGCAGA CGAAACGCTT 
GGAGAACTTT TCCTGGAAGA GAAGCCCTTT ACAGAAGACG ATATTAAGGC GGCCTTAAGG 
AGAACCTGCA TCAATAGAAC CTTCACACCT GTCTTGGTGG GCACAGCACT GAAGAACAAA 
GGTGTCCAGC CATTGCTAGA TGCAGTACTT GACTACCTGC CCAATCCTGG AGAGGTAGAG 
AACCTGGGCT TCATCGAGAA GGAAGGACAA GACCCAGAAA AAGTAGTCTT AAATCCCGCC 
CGCGATGGCA AGGATCCCTT TGTTGGCTTG GCGTTCAAGC TGGAAGCTGG TCGCTTTGGC 
CAACTAACCT ACCTCAGATG CTACCAAGGT GTCCTACGGA AGGGTGACAA CATCTTCAAT 
GCCCGCACCA ACAAGAAAGT GAGAATTGCT CGCTTGGTTC GATTGCACTC AAATCAAATG 
GAGGATGTAA ACGAGGTGTA TGCCGGCGAT ATATTTGCGC TTTTCGGAGT TGATTGCGCC 
TCCGGAGACA CTTTCACCAC CAATCCCAAG AACAATCTGT CTATGGAATC CATCTTTGTA 
CCCGAGCCCG TGGTCTCAAT GGCCATTAAG CCAAATAATA CCAAGGATAG GGATAATTTC 
TCGAAGGCTA TTGCGAGATT CACCAAAGAG GATCCCACAT TCCACTTCTT TTTCGACAAC 
GATGTAAAGG AAACTCTTGT CTCGGGAATG GGTGAACTGC ATTTGGAGAT CTATGCCCAG 
AGAATGGAGA GGGAATACGG TTGCCCAGTG ACGCTGGGAA AGCCAAAAGT AGCATTTAGG 
GAAACCCTGG TGGGACCGTG TGAGTTTGAT TACCTGCACA AGAAACAATC GGGAGGATCG 
GGTCAATATG CCAGGATTAT TGGAGTCATG GAACCACTAC CGCCGAACCA AAACACCCTG 
CTGGAATTCG TGGACGAAAC AGTGGGCACC AATGTACCAA AACAATTTGT TCCTGGAGTC 
GAAAAGGGTT ACAGAGAAAT GGCTGAGAAG GGAATGCTCT CCGGTCACAA GTTGTCCGGC 
ATTCGATTCC GCCTGCAAGA TGGTGGTCAC CACATCGTGG ACTCCAGCGA GCTCGCCTTC 
ATGTTGGCTG CCCATGGTGC CATCAAAGAG GTCTTCCAGA ACGGCAGCTG GCAAATCCTA 
GAGCCCATTA TGCTGGTCGA GGTCACTGCA CCCGAGGAAT TCCAGGGTGC TGTAATGGGT 
CACTTGAGCA AACGGCATGG TATCATCACC GGAACTGAGG GAACCGAGGG TTGGTTCACT 
GTCTACGCGG AGGTACCGCT GAACGACATG TTTGGCTATG CGGGAGAGCT GAGATCGAGT 
ACCCAGGGAA AGGGTGAATT CACCATGGAG TACTCCAGGT ACTCGCCTTG TCTCCCAGAT 
GTCCAGGATC AGATCGTCCG GCAATACCAG GAGTCGCAAG GATTGGCCCA ACCCGACAAG 
AAGAAAAAGA AGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609105.1
CDS	102..2339
Translation	MSLITRLLTGNNTLRLRALKSLGKAGYSSHAKFSEHKPIERIRNIGISAHIDSGKTTLTERILFYTGRIAEMHEVRGKDNVGATMDSMELERQRGITIQSAATYTLWKDTNINIIDTPGHVDFTVEVERALRVLDGAVLVLCAVGGVQSQTLTVNRQMKRYNVPCLAFINKLDRLGSNPYRVLSQMRSKMNHNAAFIQLPIGVESNCKGIVDLVREKAIYFEGEHGMDIRLDEIPQDMRVESLERRQELIEHLSNADETLGELFLEEKPFTEDDIKAALRRTCINRTFTPVLVGTALKNKGVQPLLDAVLDYLPNPGEVENLGFIEKEGQDPEKVVLNPARDGKDPFVGLAFKLEAGRFGQLTYLRCYQGVLRKGDNIFNARTNKKVRIARLVRLHSNQMEDVNEVYAGDIFALFGVDCASGDTFTTNPKNNLSMESIFVPEPVVSMAIKPNNTKDRDNFSKAIARFTKEDPTFHFFFDNDVKETLVSGMGELHLEIYAQRMEREYGCPVTLGKPKVAFRETLVGPCEFDYLHKKQSGGSGQYARIIGVMEPLPPNQNTLLEFVDETVGTNVPKQFVPGVEKGYREMAEKGMLSGHKLSGIRFRLQDGGHHIVDSSELAFMLAAHGAIKEVFQNGSWQILEPIMLVEVTAPEEFQGAVMGHLSKRHGIITGTEGTEGWFTVYAEVPLNDMFGYAGELRSSTQGKGEFTMEYSRYSPCLPDVQDQIVRQYQESQGLAQPDKKKKKN

Target ORF information:

RefSeq Version	NM_135261.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial translation elongation factor G 1 (mEFG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135261.2

ORF Insert Sequence:

ATGTCGCTAA TTACCCGTTT GCTGACCGGG AACAACACCC TACGCCTCCG GGCATTGAAA 
TCACTGGGAA AGGCCGGCTA CAGTTCGCAT GCGAAGTTTT CGGAGCACAA GCCCATCGAG 
AGGATCCGGA ACATAGGAAT CTCGGCGCAT ATTGACAGTG GAAAGACGAC CTTGACGGAG 
AGGATTCTGT TCTACACCGG CAGGATTGCG GAGATGCACG AGGTGCGAGG AAAGGATAAC 
GTGGGTGCCA CCATGGACAG CATGGAGCTG GAAAGGCAGC GGGGCATTAC CATTCAATCG 
GCAGCCACGT ACACACTGTG GAAGGACACC AATATCAATA TTATCGATAC ACCCGGGCAT 
GTGGACTTCA CCGTGGAGGT GGAGCGCGCA CTCCGAGTTC TCGATGGTGC CGTTCTGGTC 
TTGTGCGCCG TGGGAGGAGT CCAGAGTCAG ACCCTGACGG TTAACCGGCA GATGAAGCGC 
TACAATGTGC CCTGCCTGGC TTTCATCAAC AAACTGGATC GCTTGGGGTC GAACCCGTAC 
AGAGTTCTAT CCCAGATGAG GTCAAAAATG AACCACAACG CTGCTTTCAT TCAGCTGCCC 
ATTGGAGTGG AGAGCAATTG CAAGGGCATC GTGGATTTGG TGCGGGAAAA AGCCATATAC 
TTTGAGGGCG AGCACGGAAT GGACATCAGG CTGGACGAAA TTCCACAGGA TATGCGGGTA 
GAGAGCCTGG AGAGAAGACA GGAGCTTATC GAACACTTGT CAAATGCAGA CGAAACGCTT 
GGAGAACTTT TCCTGGAAGA GAAGCCCTTT ACAGAAGACG ATATTAAGGC GGCCTTAAGG 
AGAACCTGCA TCAATAGAAC CTTCACACCT GTCTTGGTGG GCACAGCACT GAAGAACAAA 
GGTGTCCAGC CATTGCTAGA TGCAGTACTT GACTACCTGC CCAATCCTGG AGAGGTAGAG 
AACCTGGGCT TCATCGAGAA GGAAGGACAA GACCCAGAAA AAGTAGTCTT AAATCCCGCC 
CGCGATGGCA AGGATCCCTT TGTTGGCTTG GCGTTCAAGC TGGAAGCTGG TCGCTTTGGC 
CAACTAACCT ACCTCAGATG CTACCAAGGT GTCCTACGGA AGGGTGACAA CATCTTCAAT 
GCCCGCACCA ACAAGAAAGT GAGAATTGCT CGCTTGGTTC GATTGCACTC AAATCAAATG 
GAGGATGTAA ACGAGGTGTA TGCCGGCGAT ATATTTGCGC TTTTCGGAGT TGATTGCGCC 
TCCGGAGACA CTTTCACCAC CAATCCCAAG AACAATCTGT CTATGGAATC CATCTTTGTA 
CCCGAGCCCG TGGTCTCAAT GGCCATTAAG CCAAATAATA CCAAGGATAG GGATAATTTC 
TCGAAGGCTA TTGCGAGATT CACCAAAGAG GATCCCACAT TCCACTTCTT TTTCGACAAC 
GATGTAAAGG AAACTCTTGT CTCGGGAATG GGTGAACTGC ATTTGGAGAT CTATGCCCAG 
AGAATGGAGA GGGAATACGG TTGCCCAGTG ACGCTGGGAA AGCCAAAAGT AGCATTTAGG 
GAAACCCTGG TGGGACCGTG TGAGTTTGAT TACCTGCACA AGAAACAATC GGGAGGATCG 
GGTCAATATG CCAGGATTAT TGGAGTCATG GAACCACTAC CGCCGAACCA AAACACCCTG 
CTGGAATTCG TGGACGAAAC AGTGGGCACC AATGTACCAA AACAATTTGT TCCTGGAGTC 
GAAAAGGGTT ACAGAGAAAT GGCTGAGAAG GGAATGCTCT CCGGTCACAA GTTGTCCGGC 
ATTCGATTCC GCCTGCAAGA TGGTGGTCAC CACATCGTGG ACTCCAGCGA GCTCGCCTTC 
ATGTTGGCTG CCCATGGTGC CATCAAAGAG GTCTTCCAGA ACGGCAGCTG GCAAATCCTA 
GAGCCCATTA TGCTGGTCGA GGTCACTGCA CCCGAGGAAT TCCAGGGTGC TGTAATGGGT 
CACTTGAGCA AACGGCATGG TATCATCACC GGAACTGAGG GAACCGAGGG TTGGTTCACT 
GTCTACGCGG AGGTACCGCT GAACGACATG TTTGGCTATG CGGGAGAGCT GAGATCGAGT 
ACCCAGGGAA AGGGTGAATT CACCATGGAG TACTCCAGGT ACTCGCCTTG TCTCCCAGAT 
GTCCAGGATC AGATCGTCCG GCAATACCAG GAGTCGCAAG GATTGGCCCA ACCCGACAAG 
AAGAAAAAGA AGAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mEFG1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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