smt3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	smt3
Entrez Gene ID	33981
Full Name	CG4494 gene product from transcript CG4494-RA
Synonyms	CG4494,Dm0342,DmSUMO-1,DmSmt3,Dmel\CG4494,Dmsmt3,SMT3,SUMO,Smt3,Sumo,anon-EST:Posey240,dSmt3,dsmt3,l(2)04493,l(2)SH0182,l(2)SH2 0182,sumo
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 27C7-27C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_058063.4	NP_477411.1	smt3, isoform A
NM_001316383.1	NP_001303312.1	smt3, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	SUMO3	NP_008867.2
	SUMO3	XP_001115047.1
Bos taurus (cattle)	LOC784603	XP_005216162.1
Rattus norvegicus (Norway rat)	Sumo3	NP_001019466.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000852	XP_316822.4
Xenopus tropicalis (tropical clawed frog)	sumo3	NP_001004853.1
Pan troglodytes (chimpanzee)	SUMO3	XP_003949790.1
Danio rerio (zebrafish)	sumo3b	NP_001002677.2
Bos taurus (cattle)	SUMO3	NP_001069917.1
Mus musculus (house mouse)	Sumo3	NP_064313.1
Gallus gallus (chicken)	SUMO3	NP_001072966.1
Danio rerio (zebrafish)	sumo3a	NP_998289.1
Drosophila melanogaster (fruit fly)	smt3	NP_477411.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Smt3, a homolog of small ubiquitin-like modifier (sumo), is a non-canonical RIDD target in Drosophila S2 cells. Unlike other RIDD targets, the sumo transcript does not stably associate with the ER membrane
Title: Regulation of sumo mRNA during endoplasmic reticulum stress.

SUMO conjugation is required for the assembly of Su(Hw) and Mod(mdg4) into insulator bodies that facilitate insulator complex formation.
Title: SUMO conjugation is required for the assembly of Drosophila Su(Hw) and Mod(mdg4) into insulator bodies that facilitate insulator complex formation.

although knockdown of the homeodomain-interacting protein kinase (Hipk) suppresses Smt3 depletion-induced activation of JNK, Hipk overexpression synergistically enhances this type of JNK activation
Title: Drosophila Smt3 negatively regulates JNK signaling through sequestering Hipk in the nucleus.

SUMO coordinates multiple regulatory processes during oogenesis and early embryogenesis
Title: Genetic and proteomic evidence for roles of Drosophila SUMO in cell cycle control, Ras signaling, and early pattern formation.

Drosophila p53 modification by SUMO modulates its transactivation and pro-apoptotic functions
Title: Modification of Drosophila p53 by SUMO modulates its transactivation and pro-apoptotic functions.

The following smt3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smt3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13985	NM_001316383.1 Latest version!	Drosophila melanogaster smt3, transcript variant B (smt3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa13985	NM_058063.4 Latest version!	Drosophila melanogaster smt3, transcript variant A (smt3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smt3 ( NM_058063.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477411.1 Drosophila melanogaster smt3, transcript variant A (smt3), mRNA.

Price & Availability↑

CloneID	OFa13985
Clone ID Related Accession (Same CDS sequence)	NM_058063.4 , NM_001316383.1
Accession Version	NM_058063.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	smt3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_058063.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGACG AAAAGAAGGG AGGTGAGACC GAGCACATCA ACCTGAAGGT CCTCGGCCAG 
GACAACGCCG TCGTCCAGTT CAAGATCAAG AAGCACACAC CCTTGAGGAA GCTGATGAAC 
GCCTACTGCG ACCGTGCCGG ACTCTCCATG CAGGTGGTGC GCTTCCGTTT CGACGGACAG 
CCCATCAACG AGAACGACAC TCCGACCTCG CTGGAGATGG AGGAGGGCGA CACCATCGAG 
GTTTACCAGC AGCAGACTGG TGGCGCTCCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477411.1
CDS	223..495
Translation	MSDEKKGGETEHINLKVLGQDNAVVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGGAP

Target ORF information:

RefSeq Version	NM_058063.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster smt3, transcript variant A (smt3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058063.4

ORF Insert Sequence:

ATGTCTGACG AAAAGAAGGG AGGTGAGACC GAGCACATCA ACCTGAAGGT CCTCGGCCAG 
GACAACGCCG TCGTCCAGTT CAAGATCAAG AAGCACACAC CCTTGAGGAA GCTGATGAAC 
GCCTACTGCG ACCGTGCCGG ACTCTCCATG CAGGTGGTGC GCTTCCGTTT CGACGGACAG 
CCCATCAACG AGAACGACAC TCCGACCTCG CTGGAGATGG AGGAGGGCGA CACCATCGAG 
GTTTACCAGC AGCAGACTGG TGGCGCTCCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smt3 ( NM_001316383.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303312.1 Drosophila melanogaster smt3, transcript variant B (smt3), mRNA.

Price & Availability↑

CloneID	OFa13985
Clone ID Related Accession (Same CDS sequence)	NM_058063.4 , NM_001316383.1
Accession Version	NM_001316383.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	smt3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGACG AAAAGAAGGG AGGTGAGACC GAGCACATCA ACCTGAAGGT CCTCGGCCAG 
GACAACGCCG TCGTCCAGTT CAAGATCAAG AAGCACACAC CCTTGAGGAA GCTGATGAAC 
GCCTACTGCG ACCGTGCCGG ACTCTCCATG CAGGTGGTGC GCTTCCGTTT CGACGGACAG 
CCCATCAACG AGAACGACAC TCCGACCTCG CTGGAGATGG AGGAGGGCGA CACCATCGAG 
GTTTACCAGC AGCAGACTGG TGGCGCTCCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303312.1
CDS	169..441
Translation	MSDEKKGGETEHINLKVLGQDNAVVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQPINENDTPTSLEMEEGDTIEVYQQQTGGAP

Target ORF information:

RefSeq Version	NM_001316383.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster smt3, transcript variant B (smt3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316383.1

ORF Insert Sequence:

ATGTCTGACG AAAAGAAGGG AGGTGAGACC GAGCACATCA ACCTGAAGGT CCTCGGCCAG 
GACAACGCCG TCGTCCAGTT CAAGATCAAG AAGCACACAC CCTTGAGGAA GCTGATGAAC 
GCCTACTGCG ACCGTGCCGG ACTCTCCATG CAGGTGGTGC GCTTCCGTTT CGACGGACAG 
CCCATCAACG AGAACGACAC TCCGACCTCG CTGGAGATGG AGGAGGGCGA CACCATCGAG 
GTTTACCAGC AGCAGACTGG TGGCGCTCCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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smt3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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