Wee1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wee1
Entrez Gene ID	33965
Full Name	Wee1 kinase
Synonyms	CG4488,Dmel\CG4488,Dwee,Dwee1,WEE1,Wee,Wee 1,dWee1,dwee1,wee,wee1
General protein information	Preferred Names Wee1 kinase Names CG4488 gene product from transcript CG4488-RB CG4488-PA CG4488-PB Wee1 ortholog Wee1-PA Wee1-PB wee
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 27C4-27C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_057687.4	NP_477035.1	Wee1 kinase, isoform A
NM_001273238.1	NP_001260167.1	Wee1 kinase, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wee1	NP_033542.2
Rattus norvegicus (Norway rat)	Wee1	NP_001012760.1
Danio rerio (zebrafish)	wee1	NP_001005770.1
Homo sapiens (human)	WEE1	NP_003381.1
	WEE1	XP_002808123.1
Drosophila melanogaster (fruit fly)	wee	NP_001260167.1
Pan troglodytes (chimpanzee)	WEE1	XP_521839.2
Canis lupus familiaris (dog)	WEE1	XP_534051.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007798	XP_317709.4
Gallus gallus (chicken)	WEE1	NP_001026352.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Relationships between DNA replication, chromosome condensation, and anaphase entry are mediated by the cell cycle kinases Grp (Chk1) and dWee1 (Wee1).
Title: Chk1 and Wee1 kinases coordinate DNA replication, chromosome condensation, and anaphase entry.

Phosphorylation of the KLP61F catalytic domain by dWee1 is important for the motor's function.
Title: Tyrosines in the kinesin-5 head domain are necessary for phosphorylation by Wee1 and for mitotic spindle integrity.

Drosophila Wee1 interacts with members of the gammaTURC and is required for proper mitotic-spindle morphogenesis and positioning.
Title: Drosophila Wee1 interacts with members of the gammaTURC and is required for proper mitotic-spindle morphogenesis and positioning.

Wee1 kinases can regulate mitotic entry in vivo during metazoan development even in cycles that lack a G2 phase.
Title: Drosophila Wee1 kinase regulates Cdk1 and mitotic entry during embryogenesis.

The following Wee1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wee1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13969	NM_057687.4 Latest version!	Drosophila melanogaster Wee1 kinase (Wee1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13969	NM_001273238.1 Latest version!	Drosophila melanogaster Wee1 kinase (Wee1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wee1 ( NM_057687.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477035.1 Drosophila melanogaster Wee1 kinase (Wee1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13969
Clone ID Related Accession (Same CDS sequence)	NM_057687.4 , NM_001273238.1
Accession Version	NM_057687.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Wee1 kinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057687.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATTCC GCCAGTCGGA GCACGAGATG AGCGTCACCT CACTCGATTC AAGCGTCGAA 
CTGAGATCGC GTTCGCCATC GCCGCAGGTC TTCAATCCGC GCAAGCTCCG CTTCGCCGAT 
GATGACTTTG ACAAGGACAC GCCCGAGGGA GCGAGTCCGC AGCATCCCCT TCAGCAGCGG 
CCGAAGCTAT CCTCCGGTGA GGAGCAGCAG CTGGACAGTA AGATAGGCAA AGAAGGCGGC 
GACGGGGACG TGTCCATGTC GCCGCCATGC CAAAAGGTGC GGGCCCTGCG ACTCTTTAGC 
ACACCGGCCA CTCCAAAGAC CATCCTGCAG AAGTCGACGA CGCAGTGCAG CAACCATCTG 
TCGGCGGCAG CAGCGGCAGT GAATGCCTCC AGGCGCAGCG ACGACCTCTT CCGCCTGTCA 
GAGCGCCCGC GATCTCTGCC ACTGCACAAC CGCAAACTGC CCACCCAGGA CACGGCAAAT 
GTGAACCCCT TCACGCCAGA TAGCCTCATG GCTCACAATA AGAAGCGCTG TCGCACGCAA 
TTCGGACGCG AAAACCTCAA CCTGAACGTG AATGCTATGC AAAAGTATTT GCTCAGTGAT 
GCCTGCGACG ACGATGTGAC GGAGGAGGCG GGCGACTCGA TGCGCGAAAT CCACCAGCAG 
GCACCCAAGC GGCTGGCCCT GCACGACACG AACATCAGTC GCTTCAAGCG GGAGTTCATG 
CAAGTCAACG TAATCGGCGT TGGCGAGTTC GGTGTGGTGT TCCAATGCGT CAATCGACTA 
GATGGCTGCA TATATGCGAT TAAAAAGAGT AAGAAGCCGG TGGCAGGCAG TTCTTTCGAA 
AAGAGAGCCC TGAACGAGGT CTGGGCCCAT GCTGTATTGG GCAAGCACGA TAACGTGGTT 
AGATACTATT CCGCGTGGGC GGAGGACGAT CATATGCTGA TACAGAATGA ATTCTGCGAT 
GGTGGCAGCT TGCACGCGCG CATCCAAGAT CATTGTTTGG GTGAGGCAGA GCTAAAGATT 
GTTCTCATGC ATGTGATAGA GGGCCTACGC TATATTCATT CAAATGACCT GGTGCACATG 
GATCTGAAGC CCGAAAACAT ATTCTCCACC ATGAATCCCA ATGCACACAA GCTGGTGGAG 
GTGCAGCCGC AGCAGACCAA AGATGACGAT GGCATGGACA GCGTCTATGA AGAACTGCGC 
CATTCAGAGA ATCTGGTTAC GTACAAGATC GGCGACCTGG GACACGTGAC CTCCGTTAAG 
GAACCCTACG TCGAGGAGGG AGACTGTCGT TACCTGCCCA AGGAGATCCT TCACGAGGAT 
TATTCGAATC TCTTCAAGGC CGACATATTT TCCCTGGGCA TCACGCTGTT CGAGGCGGCC 
GGCGGTGGTC CGCTGCCTAA AAATGGACCC GAATGGCACA ACTTGCGAGA TGGCAAGGTG 
CCGATTCTGC CGAGCCTGAG CAGGGATTTT AACGAGCTCA TAGCCCAGAT GATGCATCCT 
TATCCCGACA AGAGGCCCAC TTCGCAGTCT ATATTCAGTC ATCCAATTCT GAGTGCTGTT 
GACTCCAAGA GCAAGCTGCA GCTCGGCCTG GAGCTGACCG TGGAGAAGCG CAAGAACGAG 
ATACTGATGA ACAAGCTGAG AGAGGCCAAA AAACAGATTA AACTGCTCGA GCAACGAGTC 
AATCTTCTTG CGGTGACTAA CAATCCGGAC AGTCTGGATG GACAGCGTTG CCTTCGCAGC 
TTCACGCGAC GCATGCGCAC GCCATTCTCC AGCCATGGCA AGTTCGACAG CATATCCGAT 
CGCAACAAGA ACGTTATCAC CAATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477035.1
CDS	212..2041
Translation	MAFRQSEHEMSVTSLDSSVELRSRSPSPQVFNPRKLRFADDDFDKDTPEGASPQHPLQQRPKLSSGEEQQLDSKIGKEGGDGDVSMSPPCQKVRALRLFSTPATPKTILQKSTTQCSNHLSAAAAAVNASRRSDDLFRLSERPRSLPLHNRKLPTQDTANVNPFTPDSLMAHNKKRCRTQFGRENLNLNVNAMQKYLLSDACDDDVTEEAGDSMREIHQQAPKRLALHDTNISRFKREFMQVNVIGVGEFGVVFQCVNRLDGCIYAIKKSKKPVAGSSFEKRALNEVWAHAVLGKHDNVVRYYSAWAEDDHMLIQNEFCDGGSLHARIQDHCLGEAELKIVLMHVIEGLRYIHSNDLVHMDLKPENIFSTMNPNAHKLVEVQPQQTKDDDGMDSVYEELRHSENLVTYKIGDLGHVTSVKEPYVEEGDCRYLPKEILHEDYSNLFKADIFSLGITLFEAAGGGPLPKNGPEWHNLRDGKVPILPSLSRDFNELIAQMMHPYPDKRPTSQSIFSHPILSAVDSKSKLQLGLELTVEKRKNEILMNKLREAKKQIKLLEQRVNLLAVTNNPDSLDGQRCLRSFTRRMRTPFSSHGKFDSISDRNKNVITNI

Target ORF information:

RefSeq Version	NM_057687.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Wee1 kinase (Wee1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057687.4

ORF Insert Sequence:

ATGGCATTCC GCCAGTCGGA GCACGAGATG AGCGTCACCT CACTCGATTC AAGCGTCGAA 
CTGAGATCGC GTTCGCCATC GCCGCAGGTC TTCAATCCGC GCAAGCTCCG CTTCGCCGAT 
GATGACTTTG ACAAGGACAC GCCCGAGGGA GCGAGTCCGC AGCATCCCCT TCAGCAGCGG 
CCGAAGCTAT CCTCCGGTGA GGAGCAGCAG CTGGACAGTA AGATAGGCAA AGAAGGCGGC 
GACGGGGACG TGTCCATGTC GCCGCCATGC CAAAAGGTGC GGGCCCTGCG ACTCTTTAGC 
ACACCGGCCA CTCCAAAGAC CATCCTGCAG AAGTCGACGA CGCAGTGCAG CAACCATCTG 
TCGGCGGCAG CAGCGGCAGT GAATGCCTCC AGGCGCAGCG ACGACCTCTT CCGCCTGTCA 
GAGCGCCCGC GATCTCTGCC ACTGCACAAC CGCAAACTGC CCACCCAGGA CACGGCAAAT 
GTGAACCCCT TCACGCCAGA TAGCCTCATG GCTCACAATA AGAAGCGCTG TCGCACGCAA 
TTCGGACGCG AAAACCTCAA CCTGAACGTG AATGCTATGC AAAAGTATTT GCTCAGTGAT 
GCCTGCGACG ACGATGTGAC GGAGGAGGCG GGCGACTCGA TGCGCGAAAT CCACCAGCAG 
GCACCCAAGC GGCTGGCCCT GCACGACACG AACATCAGTC GCTTCAAGCG GGAGTTCATG 
CAAGTCAACG TAATCGGCGT TGGCGAGTTC GGTGTGGTGT TCCAATGCGT CAATCGACTA 
GATGGCTGCA TATATGCGAT TAAAAAGAGT AAGAAGCCGG TGGCAGGCAG TTCTTTCGAA 
AAGAGAGCCC TGAACGAGGT CTGGGCCCAT GCTGTATTGG GCAAGCACGA TAACGTGGTT 
AGATACTATT CCGCGTGGGC GGAGGACGAT CATATGCTGA TACAGAATGA ATTCTGCGAT 
GGTGGCAGCT TGCACGCGCG CATCCAAGAT CATTGTTTGG GTGAGGCAGA GCTAAAGATT 
GTTCTCATGC ATGTGATAGA GGGCCTACGC TATATTCATT CAAATGACCT GGTGCACATG 
GATCTGAAGC CCGAAAACAT ATTCTCCACC ATGAATCCCA ATGCACACAA GCTGGTGGAG 
GTGCAGCCGC AGCAGACCAA AGATGACGAT GGCATGGACA GCGTCTATGA AGAACTGCGC 
CATTCAGAGA ATCTGGTTAC GTACAAGATC GGCGACCTGG GACACGTGAC CTCCGTTAAG 
GAACCCTACG TCGAGGAGGG AGACTGTCGT TACCTGCCCA AGGAGATCCT TCACGAGGAT 
TATTCGAATC TCTTCAAGGC CGACATATTT TCCCTGGGCA TCACGCTGTT CGAGGCGGCC 
GGCGGTGGTC CGCTGCCTAA AAATGGACCC GAATGGCACA ACTTGCGAGA TGGCAAGGTG 
CCGATTCTGC CGAGCCTGAG CAGGGATTTT AACGAGCTCA TAGCCCAGAT GATGCATCCT 
TATCCCGACA AGAGGCCCAC TTCGCAGTCT ATATTCAGTC ATCCAATTCT GAGTGCTGTT 
GACTCCAAGA GCAAGCTGCA GCTCGGCCTG GAGCTGACCG TGGAGAAGCG CAAGAACGAG 
ATACTGATGA ACAAGCTGAG AGAGGCCAAA AAACAGATTA AACTGCTCGA GCAACGAGTC 
AATCTTCTTG CGGTGACTAA CAATCCGGAC AGTCTGGATG GACAGCGTTG CCTTCGCAGC 
TTCACGCGAC GCATGCGCAC GCCATTCTCC AGCCATGGCA AGTTCGACAG CATATCCGAT 
CGCAACAAGA ACGTTATCAC CAATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wee1 ( NM_001273238.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260167.1 Drosophila melanogaster Wee1 kinase (Wee1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13969
Clone ID Related Accession (Same CDS sequence)	NM_057687.4 , NM_001273238.1
Accession Version	NM_001273238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Wee1 kinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATTCC GCCAGTCGGA GCACGAGATG AGCGTCACCT CACTCGATTC AAGCGTCGAA 
CTGAGATCGC GTTCGCCATC GCCGCAGGTC TTCAATCCGC GCAAGCTCCG CTTCGCCGAT 
GATGACTTTG ACAAGGACAC GCCCGAGGGA GCGAGTCCGC AGCATCCCCT TCAGCAGCGG 
CCGAAGCTAT CCTCCGGTGA GGAGCAGCAG CTGGACAGTA AGATAGGCAA AGAAGGCGGC 
GACGGGGACG TGTCCATGTC GCCGCCATGC CAAAAGGTGC GGGCCCTGCG ACTCTTTAGC 
ACACCGGCCA CTCCAAAGAC CATCCTGCAG AAGTCGACGA CGCAGTGCAG CAACCATCTG 
TCGGCGGCAG CAGCGGCAGT GAATGCCTCC AGGCGCAGCG ACGACCTCTT CCGCCTGTCA 
GAGCGCCCGC GATCTCTGCC ACTGCACAAC CGCAAACTGC CCACCCAGGA CACGGCAAAT 
GTGAACCCCT TCACGCCAGA TAGCCTCATG GCTCACAATA AGAAGCGCTG TCGCACGCAA 
TTCGGACGCG AAAACCTCAA CCTGAACGTG AATGCTATGC AAAAGTATTT GCTCAGTGAT 
GCCTGCGACG ACGATGTGAC GGAGGAGGCG GGCGACTCGA TGCGCGAAAT CCACCAGCAG 
GCACCCAAGC GGCTGGCCCT GCACGACACG AACATCAGTC GCTTCAAGCG GGAGTTCATG 
CAAGTCAACG TAATCGGCGT TGGCGAGTTC GGTGTGGTGT TCCAATGCGT CAATCGACTA 
GATGGCTGCA TATATGCGAT TAAAAAGAGT AAGAAGCCGG TGGCAGGCAG TTCTTTCGAA 
AAGAGAGCCC TGAACGAGGT CTGGGCCCAT GCTGTATTGG GCAAGCACGA TAACGTGGTT 
AGATACTATT CCGCGTGGGC GGAGGACGAT CATATGCTGA TACAGAATGA ATTCTGCGAT 
GGTGGCAGCT TGCACGCGCG CATCCAAGAT CATTGTTTGG GTGAGGCAGA GCTAAAGATT 
GTTCTCATGC ATGTGATAGA GGGCCTACGC TATATTCATT CAAATGACCT GGTGCACATG 
GATCTGAAGC CCGAAAACAT ATTCTCCACC ATGAATCCCA ATGCACACAA GCTGGTGGAG 
GTGCAGCCGC AGCAGACCAA AGATGACGAT GGCATGGACA GCGTCTATGA AGAACTGCGC 
CATTCAGAGA ATCTGGTTAC GTACAAGATC GGCGACCTGG GACACGTGAC CTCCGTTAAG 
GAACCCTACG TCGAGGAGGG AGACTGTCGT TACCTGCCCA AGGAGATCCT TCACGAGGAT 
TATTCGAATC TCTTCAAGGC CGACATATTT TCCCTGGGCA TCACGCTGTT CGAGGCGGCC 
GGCGGTGGTC CGCTGCCTAA AAATGGACCC GAATGGCACA ACTTGCGAGA TGGCAAGGTG 
CCGATTCTGC CGAGCCTGAG CAGGGATTTT AACGAGCTCA TAGCCCAGAT GATGCATCCT 
TATCCCGACA AGAGGCCCAC TTCGCAGTCT ATATTCAGTC ATCCAATTCT GAGTGCTGTT 
GACTCCAAGA GCAAGCTGCA GCTCGGCCTG GAGCTGACCG TGGAGAAGCG CAAGAACGAG 
ATACTGATGA ACAAGCTGAG AGAGGCCAAA AAACAGATTA AACTGCTCGA GCAACGAGTC 
AATCTTCTTG CGGTGACTAA CAATCCGGAC AGTCTGGATG GACAGCGTTG CCTTCGCAGC 
TTCACGCGAC GCATGCGCAC GCCATTCTCC AGCCATGGCA AGTTCGACAG CATATCCGAT 
CGCAACAAGA ACGTTATCAC CAATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260167.1
CDS	212..2041
Translation	MAFRQSEHEMSVTSLDSSVELRSRSPSPQVFNPRKLRFADDDFDKDTPEGASPQHPLQQRPKLSSGEEQQLDSKIGKEGGDGDVSMSPPCQKVRALRLFSTPATPKTILQKSTTQCSNHLSAAAAAVNASRRSDDLFRLSERPRSLPLHNRKLPTQDTANVNPFTPDSLMAHNKKRCRTQFGRENLNLNVNAMQKYLLSDACDDDVTEEAGDSMREIHQQAPKRLALHDTNISRFKREFMQVNVIGVGEFGVVFQCVNRLDGCIYAIKKSKKPVAGSSFEKRALNEVWAHAVLGKHDNVVRYYSAWAEDDHMLIQNEFCDGGSLHARIQDHCLGEAELKIVLMHVIEGLRYIHSNDLVHMDLKPENIFSTMNPNAHKLVEVQPQQTKDDDGMDSVYEELRHSENLVTYKIGDLGHVTSVKEPYVEEGDCRYLPKEILHEDYSNLFKADIFSLGITLFEAAGGGPLPKNGPEWHNLRDGKVPILPSLSRDFNELIAQMMHPYPDKRPTSQSIFSHPILSAVDSKSKLQLGLELTVEKRKNEILMNKLREAKKQIKLLEQRVNLLAVTNNPDSLDGQRCLRSFTRRMRTPFSSHGKFDSISDRNKNVITNI

Target ORF information:

RefSeq Version	NM_001273238.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Wee1 kinase (Wee1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273238.1

ORF Insert Sequence:

ATGGCATTCC GCCAGTCGGA GCACGAGATG AGCGTCACCT CACTCGATTC AAGCGTCGAA 
CTGAGATCGC GTTCGCCATC GCCGCAGGTC TTCAATCCGC GCAAGCTCCG CTTCGCCGAT 
GATGACTTTG ACAAGGACAC GCCCGAGGGA GCGAGTCCGC AGCATCCCCT TCAGCAGCGG 
CCGAAGCTAT CCTCCGGTGA GGAGCAGCAG CTGGACAGTA AGATAGGCAA AGAAGGCGGC 
GACGGGGACG TGTCCATGTC GCCGCCATGC CAAAAGGTGC GGGCCCTGCG ACTCTTTAGC 
ACACCGGCCA CTCCAAAGAC CATCCTGCAG AAGTCGACGA CGCAGTGCAG CAACCATCTG 
TCGGCGGCAG CAGCGGCAGT GAATGCCTCC AGGCGCAGCG ACGACCTCTT CCGCCTGTCA 
GAGCGCCCGC GATCTCTGCC ACTGCACAAC CGCAAACTGC CCACCCAGGA CACGGCAAAT 
GTGAACCCCT TCACGCCAGA TAGCCTCATG GCTCACAATA AGAAGCGCTG TCGCACGCAA 
TTCGGACGCG AAAACCTCAA CCTGAACGTG AATGCTATGC AAAAGTATTT GCTCAGTGAT 
GCCTGCGACG ACGATGTGAC GGAGGAGGCG GGCGACTCGA TGCGCGAAAT CCACCAGCAG 
GCACCCAAGC GGCTGGCCCT GCACGACACG AACATCAGTC GCTTCAAGCG GGAGTTCATG 
CAAGTCAACG TAATCGGCGT TGGCGAGTTC GGTGTGGTGT TCCAATGCGT CAATCGACTA 
GATGGCTGCA TATATGCGAT TAAAAAGAGT AAGAAGCCGG TGGCAGGCAG TTCTTTCGAA 
AAGAGAGCCC TGAACGAGGT CTGGGCCCAT GCTGTATTGG GCAAGCACGA TAACGTGGTT 
AGATACTATT CCGCGTGGGC GGAGGACGAT CATATGCTGA TACAGAATGA ATTCTGCGAT 
GGTGGCAGCT TGCACGCGCG CATCCAAGAT CATTGTTTGG GTGAGGCAGA GCTAAAGATT 
GTTCTCATGC ATGTGATAGA GGGCCTACGC TATATTCATT CAAATGACCT GGTGCACATG 
GATCTGAAGC CCGAAAACAT ATTCTCCACC ATGAATCCCA ATGCACACAA GCTGGTGGAG 
GTGCAGCCGC AGCAGACCAA AGATGACGAT GGCATGGACA GCGTCTATGA AGAACTGCGC 
CATTCAGAGA ATCTGGTTAC GTACAAGATC GGCGACCTGG GACACGTGAC CTCCGTTAAG 
GAACCCTACG TCGAGGAGGG AGACTGTCGT TACCTGCCCA AGGAGATCCT TCACGAGGAT 
TATTCGAATC TCTTCAAGGC CGACATATTT TCCCTGGGCA TCACGCTGTT CGAGGCGGCC 
GGCGGTGGTC CGCTGCCTAA AAATGGACCC GAATGGCACA ACTTGCGAGA TGGCAAGGTG 
CCGATTCTGC CGAGCCTGAG CAGGGATTTT AACGAGCTCA TAGCCCAGAT GATGCATCCT 
TATCCCGACA AGAGGCCCAC TTCGCAGTCT ATATTCAGTC ATCCAATTCT GAGTGCTGTT 
GACTCCAAGA GCAAGCTGCA GCTCGGCCTG GAGCTGACCG TGGAGAAGCG CAAGAACGAG 
ATACTGATGA ACAAGCTGAG AGAGGCCAAA AAACAGATTA AACTGCTCGA GCAACGAGTC 
AATCTTCTTG CGGTGACTAA CAATCCGGAC AGTCTGGATG GACAGCGTTG CCTTCGCAGC 
TTCACGCGAC GCATGCGCAC GCCATTCTCC AGCCATGGCA AGTTCGACAG CATATCCGAT 
CGCAACAAGA ACGTTATCAC CAATATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Wee1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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