Nhe3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nhe3
Entrez Gene ID	33939
Full Name	Na[+]/H[+] hydrogen exchanger 3
Synonyms	CG11328,DmNHE3,Dmel\CG11328,NHE3,nhe3
General protein information	Preferred Names Na[+]/H[+] hydrogen exchanger 3 Names CG11328 gene product from transcript CG11328-RH CG11328-PD CG11328-PE CG11328-PG CG11328-PH CG11328-PI CG11328-PJ Nhe3-PD Nhe3-PE Nhe3-PG Nhe3-PH Nhe3-PI Nhe3-PJ
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26F7-26F7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103635.3	NP_001097105.3	Na[+]/H[+] hydrogen exchanger 3, isoform H
NM_164701.2	NP_723202.2	Na[+]/H[+] hydrogen exchanger 3, isoform D
NM_001273217.1	NP_001260146.1	Na[+]/H[+] hydrogen exchanger 3, isoform I
NM_001144329.2	NP_001137801.1	Na[+]/H[+] hydrogen exchanger 3, isoform G
NM_001273218.1	NP_001260147.1	Na[+]/H[+] hydrogen exchanger 3, isoform J
NM_135220.3	NP_609064.3	Na[+]/H[+] hydrogen exchanger 3, isoform E

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SLC9A6	XP_529172.3
Bos taurus (cattle)	SLC9A6	NP_001094616.1
Danio rerio (zebrafish)	slc9a6a	NP_001091726.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008718	XP_314826.4
Xenopus tropicalis (tropical clawed frog)	slc9a6	XP_002932638.2
Mus musculus (house mouse)	Slc9a6	NP_766368.2
Rattus norvegicus (Norway rat)	Slc9a6	XP_217630.3
Gallus gallus (chicken)	SLC9A6	XP_420229.3
Homo sapiens (human)	SLC9A6	NP_001036002.1
Canis lupus familiaris (dog)	SLC9A6	XP_538181.4
Drosophila melanogaster (fruit fly)	Nhe3	NP_723202.2
Caenorhabditis elegans (roundworm)	nhx-5	NP_001024721.1
	SLC9A6	XP_001101721.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nhe3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nhe3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13949	NM_001273217.1 Latest version!	Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13947	NM_001144329.2 Latest version!	Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13948	NM_001273218.1 Latest version!	Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16315	NM_001103635.3 Latest version!	Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16316	NM_135220.3 Latest version!	Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16317	NM_164701.2 Latest version!	Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nhe3 ( NM_001273217.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260146.1 Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa13949
Clone ID Related Accession (Same CDS sequence)	NM_001273217.1
Accession Version	NM_001273217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Na[+]/H[+] hydrogen exchanger 3, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGGTGG AGCCTCAGGG AGTTCCAACG TACAGCGATA AATTGCCGCC TGATACTCTT 
TGGTTTAGGT ACCCCGTTAA TCAAACAAAT GGCACAAAGC CGCCAGAGGG AATCAAGACG 
TATGCCTACG TGTTTCGAGG ACAGGTACAC GATGTGGATG AGAACGAAAT CGATCTTAAG 
GCAACGTTCG ATCCGGAGGT ATTTTTCAAT ATTATCCTGC CCCCAATTAT CTTTTATGCG 
GGCTATAGCT TGAAAAAGAA ATACTTCTTT CGCAACTTGG GTGCCATACT TACGTTTGCC 
ATTGTGGGCA CGACTTTGTC GGCCTTCCTA ATCGGAGGCT TCATGTACGG CTGTGTGAAA 
CTGATGCCAA AGTACTTGAG TAGCAGTTTC ACATTCCTGG ACTCCTTATA CTTTGGAGCT 
CTGATATCGC CCACAGATCC GCTCACCATT CTAGCCATAT TCAACGATCT TAGGGTCGAC 
GTAAACCTAT ATGCGCTAGT CTTGGGCGAA TCTGTGCTCA ACGATGCCGT GGCCATAGTC 
CTTAGTGGAG CCATTCAAAA TTATGGTGAA CACTATTCCA ATACCGGGGA ATTCGAAACT 
ACAGCTTTTC TACGCTCGTT GAGCGATTTC TTCTCCATCT TCTTACTATC CCTGATGATT 
GGCGCGGCCA TGGGATGCTT GACAGCATTG ATGACCAAAT TTACTCGGGT TCGAGACTTT 
CCTTTGCTAG AATCCGCGCT CTTCGTGCTC ATGAGCTACA GCACCTTCCT GCTGGCTGAG 
GCTACGGAAC TTACTGGTGT GGTGGCCGTG CTCTTCTGCG GCATCTGCCA GGCTCACTAC 
ACGTACAACA ACTTGTCGGA GGACTCGCGC CAAAGGACAA AACAGATATT CGAGCTCCTG 
AACTTTCTAG CCGAGAATTT TATCTTTTCC TACATTGGCG TATCGATGTT CACCTTTCCC 
AAGCATCATT TTGACGCCGG TTTCATTATA ACAGCTTTTA TTTGCGCTGC CATTGGAAGG 
GCGGTGAATG TGTATCCCTT GTCCTGGCTG CTGAACATAA AGCGAAAGCC GAAAATTTCC 
ACAAACTTTC AGCATATGTT GTTCTTTGCT GGACTTCGTG GCGCCATGTC CTTTGCCTTG 
GCCATCCGAA ATACAGTGTC GGACGAACGA CAGACGATGC TGACTGCCAC ATCGCTGATT 
GTCATCTTTA CGGTTGTAAT TCAGGGTGGG GCTGCAAATT TCCTGCTTAA TTGGTTGAAA 
ATACCCGTTG GCGTGGACGA TGAGACTGAA CAACTCAATA ACTATCAAGT GCACAGTTCT 
GATGGTTATT TGCAGGATGT GGAGGGCGGT GGCGTTAATC GCAACAAGAT GCGCTTGTCC 
GGTGGAACAG ATACGAACTT GGACACGCCC GTCGATGGTA CGAATGGCAG TTTGGGCGGA 
GCAAGCGGCG GTCGCCGGCG CAACAGCCAC GAGAAGGCCA TTCTTGCTAG GATCTGGGGA 
AACTTTGATA CCAAGTACAT GAAGCCCTTA CTGACGCACT CGCGACCCAC TTTGCTGGAG 
ACGCTGCCCG TTTGCTGCAA TCCCATTGCC AGACTGCTCA CCACCACACA GCAACTAACG 
CAGGATGGAA GCGAGTTCAG ACGCGTGGAC TCGGACTCGG ACATTTGCAT AGACAATGAT 
ACCGGGAACG GTCTCAGTCA GGATGGGTCC GGAGCGGCAC CAGGCGTGGG GCGTCGGAAC 
TCCCTGAGTC GCGTAAGTAT TAAGTACACC GCCGATAATC ACCATCTACT TGCTAGCTAC 
CGAAATATTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260146.1
CDS	189..2003
Translation	MQVEPQGVPTYSDKLPPDTLWFRYPVNQTNGTKPPEGIKTYAYVFRGQVHDVDENEIDLKATFDPEVFFNIILPPIIFYAGYSLKKKYFFRNLGAILTFAIVGTTLSAFLIGGFMYGCVKLMPKYLSSSFTFLDSLYFGALISPTDPLTILAIFNDLRVDVNLYALVLGESVLNDAVAIVLSGAIQNYGEHYSNTGEFETTAFLRSLSDFFSIFLLSLMIGAAMGCLTALMTKFTRVRDFPLLESALFVLMSYSTFLLAEATELTGVVAVLFCGICQAHYTYNNLSEDSRQRTKQIFELLNFLAENFIFSYIGVSMFTFPKHHFDAGFIITAFICAAIGRAVNVYPLSWLLNIKRKPKISTNFQHMLFFAGLRGAMSFALAIRNTVSDERQTMLTATSLIVIFTVVIQGGAANFLLNWLKIPVGVDDETEQLNNYQVHSSDGYLQDVEGGGVNRNKMRLSGGTDTNLDTPVDGTNGSLGGASGGRRRNSHEKAILARIWGNFDTKYMKPLLTHSRPTLLETLPVCCNPIARLLTTTQQLTQDGSEFRRVDSDSDICIDNDTGNGLSQDGSGAAPGVGRRNSLSRVSIKYTADNHHLLASYRNIE

Target ORF information:

RefSeq Version	NM_001273217.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273217.1

ORF Insert Sequence:

ATGCAGGTGG AGCCTCAGGG AGTTCCAACG TACAGCGATA AATTGCCGCC TGATACTCTT 
TGGTTTAGGT ACCCCGTTAA TCAAACAAAT GGCACAAAGC CGCCAGAGGG AATCAAGACG 
TATGCCTACG TGTTTCGAGG ACAGGTACAC GATGTGGATG AGAACGAAAT CGATCTTAAG 
GCAACGTTCG ATCCGGAGGT ATTTTTCAAT ATTATCCTGC CCCCAATTAT CTTTTATGCG 
GGCTATAGCT TGAAAAAGAA ATACTTCTTT CGCAACTTGG GTGCCATACT TACGTTTGCC 
ATTGTGGGCA CGACTTTGTC GGCCTTCCTA ATCGGAGGCT TCATGTACGG CTGTGTGAAA 
CTGATGCCAA AGTACTTGAG TAGCAGTTTC ACATTCCTGG ACTCCTTATA CTTTGGAGCT 
CTGATATCGC CCACAGATCC GCTCACCATT CTAGCCATAT TCAACGATCT TAGGGTCGAC 
GTAAACCTAT ATGCGCTAGT CTTGGGCGAA TCTGTGCTCA ACGATGCCGT GGCCATAGTC 
CTTAGTGGAG CCATTCAAAA TTATGGTGAA CACTATTCCA ATACCGGGGA ATTCGAAACT 
ACAGCTTTTC TACGCTCGTT GAGCGATTTC TTCTCCATCT TCTTACTATC CCTGATGATT 
GGCGCGGCCA TGGGATGCTT GACAGCATTG ATGACCAAAT TTACTCGGGT TCGAGACTTT 
CCTTTGCTAG AATCCGCGCT CTTCGTGCTC ATGAGCTACA GCACCTTCCT GCTGGCTGAG 
GCTACGGAAC TTACTGGTGT GGTGGCCGTG CTCTTCTGCG GCATCTGCCA GGCTCACTAC 
ACGTACAACA ACTTGTCGGA GGACTCGCGC CAAAGGACAA AACAGATATT CGAGCTCCTG 
AACTTTCTAG CCGAGAATTT TATCTTTTCC TACATTGGCG TATCGATGTT CACCTTTCCC 
AAGCATCATT TTGACGCCGG TTTCATTATA ACAGCTTTTA TTTGCGCTGC CATTGGAAGG 
GCGGTGAATG TGTATCCCTT GTCCTGGCTG CTGAACATAA AGCGAAAGCC GAAAATTTCC 
ACAAACTTTC AGCATATGTT GTTCTTTGCT GGACTTCGTG GCGCCATGTC CTTTGCCTTG 
GCCATCCGAA ATACAGTGTC GGACGAACGA CAGACGATGC TGACTGCCAC ATCGCTGATT 
GTCATCTTTA CGGTTGTAAT TCAGGGTGGG GCTGCAAATT TCCTGCTTAA TTGGTTGAAA 
ATACCCGTTG GCGTGGACGA TGAGACTGAA CAACTCAATA ACTATCAAGT GCACAGTTCT 
GATGGTTATT TGCAGGATGT GGAGGGCGGT GGCGTTAATC GCAACAAGAT GCGCTTGTCC 
GGTGGAACAG ATACGAACTT GGACACGCCC GTCGATGGTA CGAATGGCAG TTTGGGCGGA 
GCAAGCGGCG GTCGCCGGCG CAACAGCCAC GAGAAGGCCA TTCTTGCTAG GATCTGGGGA 
AACTTTGATA CCAAGTACAT GAAGCCCTTA CTGACGCACT CGCGACCCAC TTTGCTGGAG 
ACGCTGCCCG TTTGCTGCAA TCCCATTGCC AGACTGCTCA CCACCACACA GCAACTAACG 
CAGGATGGAA GCGAGTTCAG ACGCGTGGAC TCGGACTCGG ACATTTGCAT AGACAATGAT 
ACCGGGAACG GTCTCAGTCA GGATGGGTCC GGAGCGGCAC CAGGCGTGGG GCGTCGGAAC 
TCCCTGAGTC GCGTAAGTAT TAAGTACACC GCCGATAATC ACCATCTACT TGCTAGCTAC 
CGAAATATTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhe3 ( NM_001144329.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137801.1 Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa13947
Clone ID Related Accession (Same CDS sequence)	NM_001144329.2
Accession Version	NM_001144329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Na[+]/H[+] hydrogen exchanger 3, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Dec 17, 2009 this sequence version replaced NM_001144329.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCTCA TAGTCGGATT TGCATTGTGC GTCCTCGCGC CTGGATGCAG CGCCACGGAC 
ACTGACATCG CTCTGGACGC CAAGGCCACG CTCAATCACC GCATCCAGAG CCTGGACCTT 
CTAGTCTTCG TGTTCCTGCT GGCGCTCACA GTGCTCACAA TTTGGCTTTT CAAACACCAT 
CGCGTCTCCT GGCTCCACGA GACGGGATTG GCCGTCATCT ACGGCTTGAT TGTGGGTGCA 
ATTATACGAT ATGCCGGCAC CTCCGCCACC CTGGTCCACA TGCAGGTGGA GCCTCAGGGA 
GTTCCAACGT ACAGCGATAA ATTGCCGCCT GATACTCTTT GGTTTAGGTA CCCCGTTAAT 
CAAACAAATG GCACAAAGCC GCCAGAGGGA ATCAAGACGT ATGCCTACGT GTTTCGAGGA 
CAGGTACACG ATGTGGATGA GAACGAAATC GATCTTAAGG CAACGTTCGA TCCGGAGGTA 
TTTTTCAATA TTATCCTGCC CCCAATTATC TTTTATGCGG GCTATAGCTT GAAAAAGAAA 
TACTTCTTTC GCAACTTGGG TGCCATACTT ACGTTTGCCA TTGTGGGCAC GACTTTGTCG 
GCCTTCCTAA TCGGAGGCTT CATGTACGGC TGTGTGAAAC TGATGCCAAA GTACTTGAGT 
AGCAGTTTCA CATTCCTGGA CTCCTTATAC TTTGGAGCTC TGATATCGCC CACAGATCCG 
CTCACCATTC TAGCCATATT CAACGATCTT AGGGTCGACG TAAACCTATA TGCGCTAGTC 
TTGGGCGAAT CTGTGCTCAA CGATGCCGTG GCCATAGTCC TTAGTGGAGC CATTCAAAAT 
TATGGTGAAC ACTATTCCAA TACCGGGGAA TTCGAAACTA CAGCTTTTCT ACGCTCGTTG 
AGCGATTTCT TCTCCATCTT CTTACTATCC CTGATGATTG GCGCGGCCAT GGGATGCTTG 
ACAGCATTGA TGACCAAATT TACTCGGGTT CGAGACTTTC CTTTGCTAGA ATCCGCGCTC 
TTCGTGCTCA TGAGCTACAG CACCTTCCTG CTGGCTGAGG CTACGGAACT TACTGGTGTG 
GTGGCCGTGC TCTTCTGCGG CATCTGCCAG GCTCACTACA CGTACAACAA CTTGTCGGAG 
GACTCGCGCC AAAGGACAAA ACAGATATTC GAGCTCCTGA ACTTTCTAGC CGAGAATTTT 
ATCTTTTCCT ACATTGGCGT ATCGATGTTC ACCTTTCCCA AGCATCATTT TGACGCCGGT 
TTCATTATAA CAGCTTTTAT TTGCGCTGCC ATTGGAAGGG CGGTGAATGT GTATCCCTTG 
TCCTGGCTGC TGAACATAAA GCGAAAGCCG AAAATTTCCA CAAACTTTCA GCATATGTTG 
TTCTTTGCTG GACTTCGTGG CGCCATGTCC TTTGCCTTGG CCATCCGAAA TACAGTGTCG 
GACGAACGAC AGACGATGCT GACTGCCACA TCGCTGATTG TCATCTTTAC GGTTGTAATT 
CAGGGTGGGG CTGCAAATTT CCTGCTTAAT TGGTTGAAAA TACCCGTTGG CGTGGACGAT 
GAGACTGAAC AACTCAATAA CTATCAAGTG CACAGTGATG TGGAGGGCGG TGGCGTTAAT 
CGCAACAAGA TGCGCTTGTC CGGTGGAACA GATACGAACT TGGACACGCC CGTCGATGGT 
ACGAATGGCA GTTTGGGCGG AGCAAGCGGC GGTCGCCGGC GCAACAGCCA CGAGAAGGCC 
ATTCTTGCTA GGATCTGGGG AAACTTTGAT ACCAAGTACA TGAAGCCCTT ACTGACGCAC 
TCGCGACCCA CTTTGCTGGA GACGCTGCCC GTTTGCTGCA ATCCCATTGC CAGACTGCTC 
ACCACCACAC AGCAACTAAC GCAGGATGGA AGCGAGTTCA GACGCGTGGA CTCGGACTCG 
GACATTTGCA TAGACAATGA TACCGGGAAC GGTCTCAGTC AGGATGGGTC CGGAGCGGCA 
CCAGGCGTGG GGCGTCGGAA CTCCCTGAGT CGCGTTGGAG GCGATCATCG AAATGCTTAC 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137801.1
CDS	410..2455
Translation	MLLIVGFALCVLAPGCSATDTDIALDAKATLNHRIQSLDLLVFVFLLALTVLTIWLFKHHRVSWLHETGLAVIYGLIVGAIIRYAGTSATLVHMQVEPQGVPTYSDKLPPDTLWFRYPVNQTNGTKPPEGIKTYAYVFRGQVHDVDENEIDLKATFDPEVFFNIILPPIIFYAGYSLKKKYFFRNLGAILTFAIVGTTLSAFLIGGFMYGCVKLMPKYLSSSFTFLDSLYFGALISPTDPLTILAIFNDLRVDVNLYALVLGESVLNDAVAIVLSGAIQNYGEHYSNTGEFETTAFLRSLSDFFSIFLLSLMIGAAMGCLTALMTKFTRVRDFPLLESALFVLMSYSTFLLAEATELTGVVAVLFCGICQAHYTYNNLSEDSRQRTKQIFELLNFLAENFIFSYIGVSMFTFPKHHFDAGFIITAFICAAIGRAVNVYPLSWLLNIKRKPKISTNFQHMLFFAGLRGAMSFALAIRNTVSDERQTMLTATSLIVIFTVVIQGGAANFLLNWLKIPVGVDDETEQLNNYQVHSDVEGGGVNRNKMRLSGGTDTNLDTPVDGTNGSLGGASGGRRRNSHEKAILARIWGNFDTKYMKPLLTHSRPTLLETLPVCCNPIARLLTTTQQLTQDGSEFRRVDSDSDICIDNDTGNGLSQDGSGAAPGVGRRNSLSRVGGDHRNAYI

Target ORF information:

RefSeq Version	NM_001144329.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144329.2

ORF Insert Sequence:

ATGCTGCTCA TAGTCGGATT TGCATTGTGC GTCCTCGCGC CTGGATGCAG CGCCACGGAC 
ACTGACATCG CTCTGGACGC CAAGGCCACG CTCAATCACC GCATCCAGAG CCTGGACCTT 
CTAGTCTTCG TGTTCCTGCT GGCGCTCACA GTGCTCACAA TTTGGCTTTT CAAACACCAT 
CGCGTCTCCT GGCTCCACGA GACGGGATTG GCCGTCATCT ACGGCTTGAT TGTGGGTGCA 
ATTATACGAT ATGCCGGCAC CTCCGCCACC CTGGTCCACA TGCAGGTGGA GCCTCAGGGA 
GTTCCAACGT ACAGCGATAA ATTGCCGCCT GATACTCTTT GGTTTAGGTA CCCCGTTAAT 
CAAACAAATG GCACAAAGCC GCCAGAGGGA ATCAAGACGT ATGCCTACGT GTTTCGAGGA 
CAGGTACACG ATGTGGATGA GAACGAAATC GATCTTAAGG CAACGTTCGA TCCGGAGGTA 
TTTTTCAATA TTATCCTGCC CCCAATTATC TTTTATGCGG GCTATAGCTT GAAAAAGAAA 
TACTTCTTTC GCAACTTGGG TGCCATACTT ACGTTTGCCA TTGTGGGCAC GACTTTGTCG 
GCCTTCCTAA TCGGAGGCTT CATGTACGGC TGTGTGAAAC TGATGCCAAA GTACTTGAGT 
AGCAGTTTCA CATTCCTGGA CTCCTTATAC TTTGGAGCTC TGATATCGCC CACAGATCCG 
CTCACCATTC TAGCCATATT CAACGATCTT AGGGTCGACG TAAACCTATA TGCGCTAGTC 
TTGGGCGAAT CTGTGCTCAA CGATGCCGTG GCCATAGTCC TTAGTGGAGC CATTCAAAAT 
TATGGTGAAC ACTATTCCAA TACCGGGGAA TTCGAAACTA CAGCTTTTCT ACGCTCGTTG 
AGCGATTTCT TCTCCATCTT CTTACTATCC CTGATGATTG GCGCGGCCAT GGGATGCTTG 
ACAGCATTGA TGACCAAATT TACTCGGGTT CGAGACTTTC CTTTGCTAGA ATCCGCGCTC 
TTCGTGCTCA TGAGCTACAG CACCTTCCTG CTGGCTGAGG CTACGGAACT TACTGGTGTG 
GTGGCCGTGC TCTTCTGCGG CATCTGCCAG GCTCACTACA CGTACAACAA CTTGTCGGAG 
GACTCGCGCC AAAGGACAAA ACAGATATTC GAGCTCCTGA ACTTTCTAGC CGAGAATTTT 
ATCTTTTCCT ACATTGGCGT ATCGATGTTC ACCTTTCCCA AGCATCATTT TGACGCCGGT 
TTCATTATAA CAGCTTTTAT TTGCGCTGCC ATTGGAAGGG CGGTGAATGT GTATCCCTTG 
TCCTGGCTGC TGAACATAAA GCGAAAGCCG AAAATTTCCA CAAACTTTCA GCATATGTTG 
TTCTTTGCTG GACTTCGTGG CGCCATGTCC TTTGCCTTGG CCATCCGAAA TACAGTGTCG 
GACGAACGAC AGACGATGCT GACTGCCACA TCGCTGATTG TCATCTTTAC GGTTGTAATT 
CAGGGTGGGG CTGCAAATTT CCTGCTTAAT TGGTTGAAAA TACCCGTTGG CGTGGACGAT 
GAGACTGAAC AACTCAATAA CTATCAAGTG CACAGTGATG TGGAGGGCGG TGGCGTTAAT 
CGCAACAAGA TGCGCTTGTC CGGTGGAACA GATACGAACT TGGACACGCC CGTCGATGGT 
ACGAATGGCA GTTTGGGCGG AGCAAGCGGC GGTCGCCGGC GCAACAGCCA CGAGAAGGCC 
ATTCTTGCTA GGATCTGGGG AAACTTTGAT ACCAAGTACA TGAAGCCCTT ACTGACGCAC 
TCGCGACCCA CTTTGCTGGA GACGCTGCCC GTTTGCTGCA ATCCCATTGC CAGACTGCTC 
ACCACCACAC AGCAACTAAC GCAGGATGGA AGCGAGTTCA GACGCGTGGA CTCGGACTCG 
GACATTTGCA TAGACAATGA TACCGGGAAC GGTCTCAGTC AGGATGGGTC CGGAGCGGCA 
CCAGGCGTGG GGCGTCGGAA CTCCCTGAGT CGCGTTGGAG GCGATCATCG AAATGCTTAC 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhe3 ( NM_001273218.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260147.1 Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant J, mRNA.

Price & Availability↑

CloneID	OFa13948
Clone ID Related Accession (Same CDS sequence)	NM_001273218.1
Accession Version	NM_001273218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Na[+]/H[+] hydrogen exchanger 3, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCTCA TAGTCGGATT TGCATTGTGC GTCCTCGCGC CTGGATGCAG CGCCACGGAC 
ACTGACATCG CTCTGGACGC CAAGGCCACG CTCAATCACC GCATCCAGAG CCTGGACCTT 
CTAGTCTTCG TGTTCCTGCT GGCGCTCACA GTGCTCACAA TTTGGCTTTT CAAACACCAT 
CGCGTCTCCT GGCTCCACGA GACGGGATTG GCCGTCATCT ACGGCTTGAT TGTGGGTGCA 
ATTATACGAT ATGCCGGCAC CTCCGCCACC CTGGTCCACA TGCAGGTGGA GCCTCAGGGA 
GTTCCAACGT ACAGCGATAA ATTGCCGCCT GATACTCTTT GGTTTAGGTA CCCCGTTAAT 
CAAACAAATG GCACAAAGCC GCCAGAGGGA ATCAAGACGT ATGCCTACGT GTTTCGAGGA 
CAGGTACACG ATGTGGATGA GAACGAAATC GATCTTAAGG CAACGTTCGA TCCGGAGGTA 
TTTTTCAATA TTATCCTGCC CCCAATTATC TTTTATGCGG GCTATAGCTT GAAAAAGAAA 
TACTTCTTTC GCAACTTGGG TGCCATACTT ACGTTTGCCA TTGTGGGCAC GACTTTGTCG 
GCCTTCCTAA TCGGAGGCTT CATGTACGGC TGTGTGAAAC TGATGCCAAA GTACTTGAGT 
AGCAGTTTCA CATTCCTGGA CTCCTTATAC TTTGGAGCTC TGATATCGCC CACAGATCCG 
CTCACCATTC TAGCCATATT CAACGATCTT AGGGTCGACG TAAACCTATA TGCGCTAGTC 
TTGGGCGAAT CTGTGCTCAA CGATGCCGTG GCCATAGTCC TTAGTGGAGC CATTCAAAAT 
TATGGTGAAC ACTATTCCAA TACCGGGGAA TTCGAAACTA CAGCTTTTCT ACGCTCGTTG 
AGCGATTTCT TCTCCATCTT CTTACTATCC CTGATGATTG GCGCGGCCAT GGGATGCTTG 
ACAGCATTGA TGACCAAATT TACTCGGGTT CGAGACTTTC CTTTGCTAGA ATCCGCGCTC 
TTCGTGCTCA TGAGCTACAG CACCTTCCTG CTGGCTGAGG CTACGGAACT TACTGGTGTG 
GTGGCCGTGC TCTTCTGCGG CATCTGCCAG GCTCACTACA CGTACAACAA CTTGTCGGAG 
GACTCGCGCC AAAGGACAAA ACAGATATTC GAGCTCCTGA ACTTTCTAGC CGAGAATTTT 
ATCTTTTCCT ACATTGGCGT ATCGATGTTC ACCTTTCCCA AGCATCATTT TGACGCCGGT 
TTCATTATAA CAGCTTTTAT TTGCGCTGCC ATTGGAAGGG CGGTGAATGT GTATCCCTTG 
TCCTGGCTGC TGAACATAAA GCGAAAGCCG AAAATTTCCA CAAACTTTCA GCATATGTTG 
TTCTTTGCTG GACTTCGTGG CGCCATGTCC TTTGCCTTGG CCATCCGAAA TACAGTGTCG 
GACGAACGAC AGACGATGCT GACTGCCACA TCGCTGATTG TCATCTTTAC GGTTGTAATT 
CAGGGTGGGG CTGCAAATTT CCTGCTTAAT TGGTTGAAAA TACCCGTTGG CGTGGACGAT 
GAGACTGAAC AACTCAATAA CTATCAAGTG CACAGTTCTG ATGGTTATTT GCAGGATGTG 
GAGGGCGGTG GCGTTAATCG CAACAAGATG CGCTTGTCCG GTGGAACAGA TACGAACTTG 
GACACGCCCG TCGATGGTAC GAATGGCAGT TTGGGCGGAG CAAGCGGCGG TCGCCGGCGC 
AACAGCCACG AGAAGGCCAT TCTTGCTAGG ATCTGGGGAA ACTTTGATAC CAAGTACATG 
AAGCCCTTAC TGACGCACTC GCGACCCACT TTGCTGGAGA CGCTGCCCGT TTGCTGCAAT 
CCCATTGCCA GACTGCTCAC CACCACACAG CAACTAACGC AGGATGGAAG CGAGTTCAGA 
CGCGTGGACT CGGACTCGGA CATTTGCATA GACAATGATA CCGGGAACGG TCTCAGTCAG 
GATGGGTCCG GAGCGGCACC AGGCGTGGGG CGTCGGAACT CCCTGAGTCG CACGGAGACC 
CAGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260147.1
CDS	410..2458
Translation	MLLIVGFALCVLAPGCSATDTDIALDAKATLNHRIQSLDLLVFVFLLALTVLTIWLFKHHRVSWLHETGLAVIYGLIVGAIIRYAGTSATLVHMQVEPQGVPTYSDKLPPDTLWFRYPVNQTNGTKPPEGIKTYAYVFRGQVHDVDENEIDLKATFDPEVFFNIILPPIIFYAGYSLKKKYFFRNLGAILTFAIVGTTLSAFLIGGFMYGCVKLMPKYLSSSFTFLDSLYFGALISPTDPLTILAIFNDLRVDVNLYALVLGESVLNDAVAIVLSGAIQNYGEHYSNTGEFETTAFLRSLSDFFSIFLLSLMIGAAMGCLTALMTKFTRVRDFPLLESALFVLMSYSTFLLAEATELTGVVAVLFCGICQAHYTYNNLSEDSRQRTKQIFELLNFLAENFIFSYIGVSMFTFPKHHFDAGFIITAFICAAIGRAVNVYPLSWLLNIKRKPKISTNFQHMLFFAGLRGAMSFALAIRNTVSDERQTMLTATSLIVIFTVVIQGGAANFLLNWLKIPVGVDDETEQLNNYQVHSSDGYLQDVEGGGVNRNKMRLSGGTDTNLDTPVDGTNGSLGGASGGRRRNSHEKAILARIWGNFDTKYMKPLLTHSRPTLLETLPVCCNPIARLLTTTQQLTQDGSEFRRVDSDSDICIDNDTGNGLSQDGSGAAPGVGRRNSLSRTETQT

Target ORF information:

RefSeq Version	NM_001273218.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273218.1

ORF Insert Sequence:

ATGCTGCTCA TAGTCGGATT TGCATTGTGC GTCCTCGCGC CTGGATGCAG CGCCACGGAC 
ACTGACATCG CTCTGGACGC CAAGGCCACG CTCAATCACC GCATCCAGAG CCTGGACCTT 
CTAGTCTTCG TGTTCCTGCT GGCGCTCACA GTGCTCACAA TTTGGCTTTT CAAACACCAT 
CGCGTCTCCT GGCTCCACGA GACGGGATTG GCCGTCATCT ACGGCTTGAT TGTGGGTGCA 
ATTATACGAT ATGCCGGCAC CTCCGCCACC CTGGTCCACA TGCAGGTGGA GCCTCAGGGA 
GTTCCAACGT ACAGCGATAA ATTGCCGCCT GATACTCTTT GGTTTAGGTA CCCCGTTAAT 
CAAACAAATG GCACAAAGCC GCCAGAGGGA ATCAAGACGT ATGCCTACGT GTTTCGAGGA 
CAGGTACACG ATGTGGATGA GAACGAAATC GATCTTAAGG CAACGTTCGA TCCGGAGGTA 
TTTTTCAATA TTATCCTGCC CCCAATTATC TTTTATGCGG GCTATAGCTT GAAAAAGAAA 
TACTTCTTTC GCAACTTGGG TGCCATACTT ACGTTTGCCA TTGTGGGCAC GACTTTGTCG 
GCCTTCCTAA TCGGAGGCTT CATGTACGGC TGTGTGAAAC TGATGCCAAA GTACTTGAGT 
AGCAGTTTCA CATTCCTGGA CTCCTTATAC TTTGGAGCTC TGATATCGCC CACAGATCCG 
CTCACCATTC TAGCCATATT CAACGATCTT AGGGTCGACG TAAACCTATA TGCGCTAGTC 
TTGGGCGAAT CTGTGCTCAA CGATGCCGTG GCCATAGTCC TTAGTGGAGC CATTCAAAAT 
TATGGTGAAC ACTATTCCAA TACCGGGGAA TTCGAAACTA CAGCTTTTCT ACGCTCGTTG 
AGCGATTTCT TCTCCATCTT CTTACTATCC CTGATGATTG GCGCGGCCAT GGGATGCTTG 
ACAGCATTGA TGACCAAATT TACTCGGGTT CGAGACTTTC CTTTGCTAGA ATCCGCGCTC 
TTCGTGCTCA TGAGCTACAG CACCTTCCTG CTGGCTGAGG CTACGGAACT TACTGGTGTG 
GTGGCCGTGC TCTTCTGCGG CATCTGCCAG GCTCACTACA CGTACAACAA CTTGTCGGAG 
GACTCGCGCC AAAGGACAAA ACAGATATTC GAGCTCCTGA ACTTTCTAGC CGAGAATTTT 
ATCTTTTCCT ACATTGGCGT ATCGATGTTC ACCTTTCCCA AGCATCATTT TGACGCCGGT 
TTCATTATAA CAGCTTTTAT TTGCGCTGCC ATTGGAAGGG CGGTGAATGT GTATCCCTTG 
TCCTGGCTGC TGAACATAAA GCGAAAGCCG AAAATTTCCA CAAACTTTCA GCATATGTTG 
TTCTTTGCTG GACTTCGTGG CGCCATGTCC TTTGCCTTGG CCATCCGAAA TACAGTGTCG 
GACGAACGAC AGACGATGCT GACTGCCACA TCGCTGATTG TCATCTTTAC GGTTGTAATT 
CAGGGTGGGG CTGCAAATTT CCTGCTTAAT TGGTTGAAAA TACCCGTTGG CGTGGACGAT 
GAGACTGAAC AACTCAATAA CTATCAAGTG CACAGTTCTG ATGGTTATTT GCAGGATGTG 
GAGGGCGGTG GCGTTAATCG CAACAAGATG CGCTTGTCCG GTGGAACAGA TACGAACTTG 
GACACGCCCG TCGATGGTAC GAATGGCAGT TTGGGCGGAG CAAGCGGCGG TCGCCGGCGC 
AACAGCCACG AGAAGGCCAT TCTTGCTAGG ATCTGGGGAA ACTTTGATAC CAAGTACATG 
AAGCCCTTAC TGACGCACTC GCGACCCACT TTGCTGGAGA CGCTGCCCGT TTGCTGCAAT 
CCCATTGCCA GACTGCTCAC CACCACACAG CAACTAACGC AGGATGGAAG CGAGTTCAGA 
CGCGTGGACT CGGACTCGGA CATTTGCATA GACAATGATA CCGGGAACGG TCTCAGTCAG 
GATGGGTCCG GAGCGGCACC AGGCGTGGGG CGTCGGAACT CCCTGAGTCG CACGGAGACC 
CAGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhe3 ( NM_001103635.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097105.3 Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa16315
Clone ID Related Accession (Same CDS sequence)	NM_001103635.3
Accession Version	NM_001103635.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Na[+]/H[+] hydrogen exchanger 3, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001103635.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCTCA TAGTCGGATT TGCATTGTGC GTCCTCGCGC CTGGATGCAG CGCCACGGAC 
ACTGACATCG CTCTGGACGC CAAGGCCACG CTCAATCACC GCATCCAGAG CCTGGACCTT 
CTAGTCTTCG TGTTCCTGCT GGCGCTCACA GTGCTCACAA TTTGGCTTTT CAAACACCAT 
CGCGTCTCCT GGCTCCACGA GACGGGATTG GCCGTCATCT ACGGCTTGAT TGTGGGTGCA 
ATTATACGAT ATGCCGGCAC CTCCGCCACC CTGGTCCACA TGCAGGTGGA GCCTCAGGGA 
GTTCCAACGT ACAGCGATAA ATTGCCGCCT GATACTCTTT GGTTTAGGTA CCCCGTTAAT 
CAAACAAATG GCACAAAGCC GCCAGAGGGA ATCAAGACGT ATGCCTACGT GTTTCGAGGA 
CAGGTACACG ATGTGGATGA GAACGAAATC GATCTTAAGG CAACGTTCGA TCCGGAGGTA 
TTTTTCAATA TTATCCTGCC CCCAATTATC TTTTATGCGG GCTATAGCTT GAAAAAGAAA 
TACTTCTTTC GCAACTTGGG TGCCATACTT ACGTTTGCCA TTGTGGGCAC GACTTTGTCG 
GCCTTCCTAA TCGGAGGCTT CATGTACGGC TGTGTGAAAC TGATGCCAAA GTACTTGAGT 
AGCAGTTTCA CATTCCTGGA CTCCTTATAC TTTGGAGCTC TGATATCGCC CACAGATCCG 
CTCACCATTC TAGCCATATT CAACGATCTT AGGGTCGACG TAAACCTATA TGCGCTAGTC 
TTGGGCGAAT CTGTGCTCAA CGATGCCGTG GCCATAGTCC TTAGTGGAGC CATTCAAAAT 
TATGGTGAAC ACTATTCCAA TACCGGGGAA TTCGAAACTA CAGCTTTTCT ACGCTCGTTG 
AGCGATTTCT TCTCCATCTT CTTACTATCC CTGATGATTG GCGCGGCCAT GGGATGCTTG 
ACAGCATTGA TGACCAAATT TACTCGGGTT CGAGACTTTC CTTTGCTAGA ATCCGCGCTC 
TTCGTGCTCA TGAGCTACAG CACCTTCCTG CTGGCTGAGG CTACGGAACT TACTGGTGTG 
GTGGCCGTGC TCTTCTGCGG CATCTGCCAG GCTCACTACA CGTACAACAA CTTGTCGGAG 
GACTCGCGCC AAAGGACAAA ACAGATATTC GAGCTCCTGA ACTTTCTAGC CGAGAATTTT 
ATCTTTTCCT ACATTGGCGT ATCGATGTTC ACCTTTCCCA AGCATCATTT TGACGCCGGT 
TTCATTATAA CAGCTTTTAT TTGCGCTGCC ATTGGAAGGG CGGTGAATGT GTATCCCTTG 
TCCTGGCTGC TGAACATAAA GCGAAAGCCG AAAATTTCCA CAAACTTTCA GCATATGTTG 
TTCTTTGCTG GACTTCGTGG CGCCATGTCC TTTGCCTTGG CCATCCGAAA TACAGTGTCG 
GACGAACGAC AGACGATGCT GACTGCCACA TCGCTGATTG TCATCTTTAC GGTTGTAATT 
CAGGGTGGGG CTGCAAATTT CCTGCTTAAT TGGTTGAAAA TACCCGTTGG CGTGGACGAT 
GAGACTGAAC AACTCAATAA CTATCAAGTG CACAGTGATG TGGAGGGCGG TGGCGTTAAT 
CGCAACAAGA TGCGCTTGTC CGGTGGAACA GATACGAACT TGGACACGCC CGTCGATGGT 
ACGAATGGCA GTTTGGGCGG AGCAAGCGGC GGTCGCCGGC GCAACAGCCA CGAGAAGGCC 
ATTCTTGCTA GGATCTGGGG AAACTTTGAT ACCAAGTACA TGAAGCCCTT ACTGACGCAC 
TCGCGACCCA CTTTGCTGGA GACGCTGCCC GTTTGCTGCA ATCCCATTGC CAGACTGCTC 
ACCACCACAC AGCAACTAAC GCAGGATGGA AGCGAGTTCA GACGCGTGGA CTCGGACTCG 
GACATTTGCA TAGACAATGA TACCGGGAAC GGTCTCAGTC AGGATGGGTC CGGAGCGGCA 
CCAGGCGTGG GGCGTCGGAA CTCCCTGAGT CGCATGGAGA TTCTGGAGCA TGTCCAAAGT 
CCGGTGTCGA CGAGAATCCG GCTCTTAGGA TTTTACGGGA AAAAGTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097105.3
CDS	417..2507
Translation	MLLIVGFALCVLAPGCSATDTDIALDAKATLNHRIQSLDLLVFVFLLALTVLTIWLFKHHRVSWLHETGLAVIYGLIVGAIIRYAGTSATLVHMQVEPQGVPTYSDKLPPDTLWFRYPVNQTNGTKPPEGIKTYAYVFRGQVHDVDENEIDLKATFDPEVFFNIILPPIIFYAGYSLKKKYFFRNLGAILTFAIVGTTLSAFLIGGFMYGCVKLMPKYLSSSFTFLDSLYFGALISPTDPLTILAIFNDLRVDVNLYALVLGESVLNDAVAIVLSGAIQNYGEHYSNTGEFETTAFLRSLSDFFSIFLLSLMIGAAMGCLTALMTKFTRVRDFPLLESALFVLMSYSTFLLAEATELTGVVAVLFCGICQAHYTYNNLSEDSRQRTKQIFELLNFLAENFIFSYIGVSMFTFPKHHFDAGFIITAFICAAIGRAVNVYPLSWLLNIKRKPKISTNFQHMLFFAGLRGAMSFALAIRNTVSDERQTMLTATSLIVIFTVVIQGGAANFLLNWLKIPVGVDDETEQLNNYQVHSDVEGGGVNRNKMRLSGGTDTNLDTPVDGTNGSLGGASGGRRRNSHEKAILARIWGNFDTKYMKPLLTHSRPTLLETLPVCCNPIARLLTTTQQLTQDGSEFRRVDSDSDICIDNDTGNGLSQDGSGAAPGVGRRNSLSRMEILEHVQSPVSTRIRLLGFYGKKL

Target ORF information:

RefSeq Version	NM_001103635.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103635.3

ORF Insert Sequence:

ATGCTGCTCA TAGTCGGATT TGCATTGTGC GTCCTCGCGC CTGGATGCAG CGCCACGGAC 
ACTGACATCG CTCTGGACGC CAAGGCCACG CTCAATCACC GCATCCAGAG CCTGGACCTT 
CTAGTCTTCG TGTTCCTGCT GGCGCTCACA GTGCTCACAA TTTGGCTTTT CAAACACCAT 
CGCGTCTCCT GGCTCCACGA GACGGGATTG GCCGTCATCT ACGGCTTGAT TGTGGGTGCA 
ATTATACGAT ATGCCGGCAC CTCCGCCACC CTGGTCCACA TGCAGGTGGA GCCTCAGGGA 
GTTCCAACGT ACAGCGATAA ATTGCCGCCT GATACTCTTT GGTTTAGGTA CCCCGTTAAT 
CAAACAAATG GCACAAAGCC GCCAGAGGGA ATCAAGACGT ATGCCTACGT GTTTCGAGGA 
CAGGTACACG ATGTGGATGA GAACGAAATC GATCTTAAGG CAACGTTCGA TCCGGAGGTA 
TTTTTCAATA TTATCCTGCC CCCAATTATC TTTTATGCGG GCTATAGCTT GAAAAAGAAA 
TACTTCTTTC GCAACTTGGG TGCCATACTT ACGTTTGCCA TTGTGGGCAC GACTTTGTCG 
GCCTTCCTAA TCGGAGGCTT CATGTACGGC TGTGTGAAAC TGATGCCAAA GTACTTGAGT 
AGCAGTTTCA CATTCCTGGA CTCCTTATAC TTTGGAGCTC TGATATCGCC CACAGATCCG 
CTCACCATTC TAGCCATATT CAACGATCTT AGGGTCGACG TAAACCTATA TGCGCTAGTC 
TTGGGCGAAT CTGTGCTCAA CGATGCCGTG GCCATAGTCC TTAGTGGAGC CATTCAAAAT 
TATGGTGAAC ACTATTCCAA TACCGGGGAA TTCGAAACTA CAGCTTTTCT ACGCTCGTTG 
AGCGATTTCT TCTCCATCTT CTTACTATCC CTGATGATTG GCGCGGCCAT GGGATGCTTG 
ACAGCATTGA TGACCAAATT TACTCGGGTT CGAGACTTTC CTTTGCTAGA ATCCGCGCTC 
TTCGTGCTCA TGAGCTACAG CACCTTCCTG CTGGCTGAGG CTACGGAACT TACTGGTGTG 
GTGGCCGTGC TCTTCTGCGG CATCTGCCAG GCTCACTACA CGTACAACAA CTTGTCGGAG 
GACTCGCGCC AAAGGACAAA ACAGATATTC GAGCTCCTGA ACTTTCTAGC CGAGAATTTT 
ATCTTTTCCT ACATTGGCGT ATCGATGTTC ACCTTTCCCA AGCATCATTT TGACGCCGGT 
TTCATTATAA CAGCTTTTAT TTGCGCTGCC ATTGGAAGGG CGGTGAATGT GTATCCCTTG 
TCCTGGCTGC TGAACATAAA GCGAAAGCCG AAAATTTCCA CAAACTTTCA GCATATGTTG 
TTCTTTGCTG GACTTCGTGG CGCCATGTCC TTTGCCTTGG CCATCCGAAA TACAGTGTCG 
GACGAACGAC AGACGATGCT GACTGCCACA TCGCTGATTG TCATCTTTAC GGTTGTAATT 
CAGGGTGGGG CTGCAAATTT CCTGCTTAAT TGGTTGAAAA TACCCGTTGG CGTGGACGAT 
GAGACTGAAC AACTCAATAA CTATCAAGTG CACAGTGATG TGGAGGGCGG TGGCGTTAAT 
CGCAACAAGA TGCGCTTGTC CGGTGGAACA GATACGAACT TGGACACGCC CGTCGATGGT 
ACGAATGGCA GTTTGGGCGG AGCAAGCGGC GGTCGCCGGC GCAACAGCCA CGAGAAGGCC 
ATTCTTGCTA GGATCTGGGG AAACTTTGAT ACCAAGTACA TGAAGCCCTT ACTGACGCAC 
TCGCGACCCA CTTTGCTGGA GACGCTGCCC GTTTGCTGCA ATCCCATTGC CAGACTGCTC 
ACCACCACAC AGCAACTAAC GCAGGATGGA AGCGAGTTCA GACGCGTGGA CTCGGACTCG 
GACATTTGCA TAGACAATGA TACCGGGAAC GGTCTCAGTC AGGATGGGTC CGGAGCGGCA 
CCAGGCGTGG GGCGTCGGAA CTCCCTGAGT CGCATGGAGA TTCTGGAGCA TGTCCAAAGT 
CCGGTGTCGA CGAGAATCCG GCTCTTAGGA TTTTACGGGA AAAAGTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhe3 ( NM_135220.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609064.3 Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa16316
Clone ID Related Accession (Same CDS sequence)	NM_135220.3
Accession Version	NM_135220.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Na[+]/H[+] hydrogen exchanger 3, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Dec 17, 2009 this sequence version replaced NM_135220.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCTCA TAGTCGGATT TGCATTGTGC GTCCTCGCGC CTGGATGCAG CGCCACGGAC 
ACTGACATCG CTCTGGACGC CAAGGCCACG CTCAATCACC GCATCCAGAG CCTGGACCTT 
CTAGTCTTCG TGTTCCTGCT GGCGCTCACA GTGCTCACAA TTTGGCTTTT CAAACACCAT 
CGCGTCTCCT GGCTCCACGA GACGGGATTG GCCGTCATCT ACGGCTTGAT TGTGGGTGCA 
ATTATACGAT ATGCCGGCAC CTCCGCCACC CTGGTCCACA TGCAGGTGGA GCCTCAGGGA 
GTTCCAACGT ACAGCGATAA ATTGCCGCCT GATACTCTTT GGTTTAGGTA CCCCGTTAAT 
CAAACAAATG GCACAAAGCC GCCAGAGGGA ATCAAGACGT ATGCCTACGT GTTTCGAGGA 
CAGGTACACG ATGTGGATGA GAACGAAATC GATCTTAAGG CAACGTTCGA TCCGGAGGTA 
TTTTTCAATA TTATCCTGCC CCCAATTATC TTTTATGCGG GCTATAGCTT GAAAAAGAAA 
TACTTCTTTC GCAACTTGGG TGCCATACTT ACGTTTGCCA TTGTGGGCAC GACTTTGTCG 
GCCTTCCTAA TCGGAGGCTT CATGTACGGC TGTGTGAAAC TGATGCCAAA GTACTTGAGT 
AGCAGTTTCA CATTCCTGGA CTCCTTATAC TTTGGAGCTC TGATATCGCC CACAGATCCG 
CTCACCATTC TAGCCATATT CAACGATCTT AGGGTCGACG TAAACCTATA TGCGCTAGTC 
TTGGGCGAAT CTGTGCTCAA CGATGCCGTG GCCATAGTCC TTAGTGGAGC CATTCAAAAT 
TATGGTGAAC ACTATTCCAA TACCGGGGAA TTCGAAACTA CAGCTTTTCT ACGCTCGTTG 
AGCGATTTCT TCTCCATCTT CTTACTATCC CTGATGATTG GCGCGGCCAT GGGATGCTTG 
ACAGCATTGA TGACCAAATT TACTCGGGTT CGAGACTTTC CTTTGCTAGA ATCCGCGCTC 
TTCGTGCTCA TGAGCTACAG CACCTTCCTG CTGGCTGAGG CTACGGAACT TACTGGTGTG 
GTGGCCGTGC TCTTCTGCGG CATCTGCCAG GCTCACTACA CGTACAACAA CTTGTCGGAG 
GACTCGCGCC AAAGGACAAA ACAGATATTC GAGCTCCTGA ACTTTCTAGC CGAGAATTTT 
ATCTTTTCCT ACATTGGCGT ATCGATGTTC ACCTTTCCCA AGCATCATTT TGACGCCGGT 
TTCATTATAA CAGCTTTTAT TTGCGCTGCC ATTGGAAGGG CGGTGAATGT GTATCCCTTG 
TCCTGGCTGC TGAACATAAA GCGAAAGCCG AAAATTTCCA CAAACTTTCA GCATATGTTG 
TTCTTTGCTG GACTTCGTGG CGCCATGTCC TTTGCCTTGG CCATCCGAAA TACAGTGTCG 
GACGAACGAC AGACGATGCT GACTGCCACA TCGCTGATTG TCATCTTTAC GGTTGTAATT 
CAGGGTGGGG CTGCAAATTT CCTGCTTAAT TGGTTGAAAA TACCCGTTGG CGTGGACGAT 
GAGACTGAAC AACTCAATAA CTATCAAGTG CACAGTTCTG ATGGTTATTT GCAGGATGTG 
GAGGGCGGTG GCGTTAATCG CAACAAGATG CGCTTGTCCG GTGGAACAGA TACGAACTTG 
GACACGCCCG TCGATGGTAC GAATGGCAGT TTGGGCGGAG CAAGCGGCGG TCGCCGGCGC 
AACAGCCACG AGAAGGCCAT TCTTGCTAGG ATCTGGGGAA ACTTTGATAC CAAGTACATG 
AAGCCCTTAC TGACGCACTC GCGACCCACT TTGCTGGAGA CGCTGCCCGT TTGCTGCAAT 
CCCATTGCCA GACTGCTCAC CACCACACAG CAACTAACGC AGGATGGAAG CGAGTTCAGA 
CGCGTGGACT CGGACTCGGA CATTTGCATA GACAATGATA CCGGGAACGG TCTCAGTCAG 
GATGGGTCCG GAGCGGCACC AGGCGTGGGG CGTCGGAACT CCCTGAGTCG CGTTGGAGGC 
GATCATCGAA ATGCTTACAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609064.3
CDS	410..2473
Translation	MLLIVGFALCVLAPGCSATDTDIALDAKATLNHRIQSLDLLVFVFLLALTVLTIWLFKHHRVSWLHETGLAVIYGLIVGAIIRYAGTSATLVHMQVEPQGVPTYSDKLPPDTLWFRYPVNQTNGTKPPEGIKTYAYVFRGQVHDVDENEIDLKATFDPEVFFNIILPPIIFYAGYSLKKKYFFRNLGAILTFAIVGTTLSAFLIGGFMYGCVKLMPKYLSSSFTFLDSLYFGALISPTDPLTILAIFNDLRVDVNLYALVLGESVLNDAVAIVLSGAIQNYGEHYSNTGEFETTAFLRSLSDFFSIFLLSLMIGAAMGCLTALMTKFTRVRDFPLLESALFVLMSYSTFLLAEATELTGVVAVLFCGICQAHYTYNNLSEDSRQRTKQIFELLNFLAENFIFSYIGVSMFTFPKHHFDAGFIITAFICAAIGRAVNVYPLSWLLNIKRKPKISTNFQHMLFFAGLRGAMSFALAIRNTVSDERQTMLTATSLIVIFTVVIQGGAANFLLNWLKIPVGVDDETEQLNNYQVHSSDGYLQDVEGGGVNRNKMRLSGGTDTNLDTPVDGTNGSLGGASGGRRRNSHEKAILARIWGNFDTKYMKPLLTHSRPTLLETLPVCCNPIARLLTTTQQLTQDGSEFRRVDSDSDICIDNDTGNGLSQDGSGAAPGVGRRNSLSRVGGDHRNAYI

Target ORF information:

RefSeq Version	NM_135220.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135220.3

ORF Insert Sequence:

ATGCTGCTCA TAGTCGGATT TGCATTGTGC GTCCTCGCGC CTGGATGCAG CGCCACGGAC 
ACTGACATCG CTCTGGACGC CAAGGCCACG CTCAATCACC GCATCCAGAG CCTGGACCTT 
CTAGTCTTCG TGTTCCTGCT GGCGCTCACA GTGCTCACAA TTTGGCTTTT CAAACACCAT 
CGCGTCTCCT GGCTCCACGA GACGGGATTG GCCGTCATCT ACGGCTTGAT TGTGGGTGCA 
ATTATACGAT ATGCCGGCAC CTCCGCCACC CTGGTCCACA TGCAGGTGGA GCCTCAGGGA 
GTTCCAACGT ACAGCGATAA ATTGCCGCCT GATACTCTTT GGTTTAGGTA CCCCGTTAAT 
CAAACAAATG GCACAAAGCC GCCAGAGGGA ATCAAGACGT ATGCCTACGT GTTTCGAGGA 
CAGGTACACG ATGTGGATGA GAACGAAATC GATCTTAAGG CAACGTTCGA TCCGGAGGTA 
TTTTTCAATA TTATCCTGCC CCCAATTATC TTTTATGCGG GCTATAGCTT GAAAAAGAAA 
TACTTCTTTC GCAACTTGGG TGCCATACTT ACGTTTGCCA TTGTGGGCAC GACTTTGTCG 
GCCTTCCTAA TCGGAGGCTT CATGTACGGC TGTGTGAAAC TGATGCCAAA GTACTTGAGT 
AGCAGTTTCA CATTCCTGGA CTCCTTATAC TTTGGAGCTC TGATATCGCC CACAGATCCG 
CTCACCATTC TAGCCATATT CAACGATCTT AGGGTCGACG TAAACCTATA TGCGCTAGTC 
TTGGGCGAAT CTGTGCTCAA CGATGCCGTG GCCATAGTCC TTAGTGGAGC CATTCAAAAT 
TATGGTGAAC ACTATTCCAA TACCGGGGAA TTCGAAACTA CAGCTTTTCT ACGCTCGTTG 
AGCGATTTCT TCTCCATCTT CTTACTATCC CTGATGATTG GCGCGGCCAT GGGATGCTTG 
ACAGCATTGA TGACCAAATT TACTCGGGTT CGAGACTTTC CTTTGCTAGA ATCCGCGCTC 
TTCGTGCTCA TGAGCTACAG CACCTTCCTG CTGGCTGAGG CTACGGAACT TACTGGTGTG 
GTGGCCGTGC TCTTCTGCGG CATCTGCCAG GCTCACTACA CGTACAACAA CTTGTCGGAG 
GACTCGCGCC AAAGGACAAA ACAGATATTC GAGCTCCTGA ACTTTCTAGC CGAGAATTTT 
ATCTTTTCCT ACATTGGCGT ATCGATGTTC ACCTTTCCCA AGCATCATTT TGACGCCGGT 
TTCATTATAA CAGCTTTTAT TTGCGCTGCC ATTGGAAGGG CGGTGAATGT GTATCCCTTG 
TCCTGGCTGC TGAACATAAA GCGAAAGCCG AAAATTTCCA CAAACTTTCA GCATATGTTG 
TTCTTTGCTG GACTTCGTGG CGCCATGTCC TTTGCCTTGG CCATCCGAAA TACAGTGTCG 
GACGAACGAC AGACGATGCT GACTGCCACA TCGCTGATTG TCATCTTTAC GGTTGTAATT 
CAGGGTGGGG CTGCAAATTT CCTGCTTAAT TGGTTGAAAA TACCCGTTGG CGTGGACGAT 
GAGACTGAAC AACTCAATAA CTATCAAGTG CACAGTTCTG ATGGTTATTT GCAGGATGTG 
GAGGGCGGTG GCGTTAATCG CAACAAGATG CGCTTGTCCG GTGGAACAGA TACGAACTTG 
GACACGCCCG TCGATGGTAC GAATGGCAGT TTGGGCGGAG CAAGCGGCGG TCGCCGGCGC 
AACAGCCACG AGAAGGCCAT TCTTGCTAGG ATCTGGGGAA ACTTTGATAC CAAGTACATG 
AAGCCCTTAC TGACGCACTC GCGACCCACT TTGCTGGAGA CGCTGCCCGT TTGCTGCAAT 
CCCATTGCCA GACTGCTCAC CACCACACAG CAACTAACGC AGGATGGAAG CGAGTTCAGA 
CGCGTGGACT CGGACTCGGA CATTTGCATA GACAATGATA CCGGGAACGG TCTCAGTCAG 
GATGGGTCCG GAGCGGCACC AGGCGTGGGG CGTCGGAACT CCCTGAGTCG CGTTGGAGGC 
GATCATCGAA ATGCTTACAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhe3 ( NM_164701.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723202.2 Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant D, mRNA.

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CloneID	OFa16317
Clone ID Related Accession (Same CDS sequence)	NM_164701.2
Accession Version	NM_164701.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Na[+]/H[+] hydrogen exchanger 3, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Dec 17, 2009 this sequence version replaced NM_164701.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGCTCA TAGTCGGATT TGCATTGTGC GTCCTCGCGC CTGGATGCAG CGCCACGGAC 
ACTGACATCG CTCTGGACGC CAAGGCCACG CTCAATCACC GCATCCAGAG CCTGGACCTT 
CTAGTCTTCG TGTTCCTGCT GGCGCTCACA GTGCTCACAA TTTGGCTTTT CAAACACCAT 
CGCGTCTCCT GGCTCCACGA GACGGGATTG GCCGTCATCT ACGGCTTGAT TGTGGGTGCA 
ATTATACGAT ATGCCGGCAC CTCCGCCACC CTGGTCCACA TGCAGGTGGA GCCTCAGGGA 
GTTCCAACGT ACAGCGATAA ATTGCCGCCT GATACTCTTT GGTTTAGGTA CCCCGTTAAT 
CAAACAAATG GCACAAAGCC GCCAGAGGGA ATCAAGACGT ATGCCTACGT GTTTCGAGGA 
CAGGTACACG ATGTGGATGA GAACGAAATC GATCTTAAGG CAACGTTCGA TCCGGAGGTA 
TTTTTCAATA TTATCCTGCC CCCAATTATC TTTTATGCGG GCTATAGCTT GAAAAAGAAA 
TACTTCTTTC GCAACTTGGG TGCCATACTT ACGTTTGCCA TTGTGGGCAC GACTTTGTCG 
GCCTTCCTAA TCGGAGGCTT CATGTACGGC TGTGTGAAAC TGATGCCAAA GTACTTGAGT 
AGCAGTTTCA CATTCCTGGA CTCCTTATAC TTTGGAGCTC TGATATCGCC CACAGATCCG 
CTCACCATTC TAGCCATATT CAACGATCTT AGGGTCGACG TAAACCTATA TGCGCTAGTC 
TTGGGCGAAT CTGTGCTCAA CGATGCCGTG GCCATAGTCC TTAGTGGAGC CATTCAAAAT 
TATGGTGAAC ACTATTCCAA TACCGGGGAA TTCGAAACTA CAGCTTTTCT ACGCTCGTTG 
AGCGATTTCT TCTCCATCTT CTTACTATCC CTGATGATTG GCGCGGCCAT GGGATGCTTG 
ACAGCATTGA TGACCAAATT TACTCGGGTT CGAGACTTTC CTTTGCTAGA ATCCGCGCTC 
TTCGTGCTCA TGAGCTACAG CACCTTCCTG CTGGCTGAGG CTACGGAACT TACTGGTGTG 
GTGGCCGTGC TCTTCTGCGG CATCTGCCAG GCTCACTACA CGTACAACAA CTTGTCGGAG 
GACTCGCGCC AAAGGACAAA ACAGATATTC GAGCTCCTGA ACTTTCTAGC CGAGAATTTT 
ATCTTTTCCT ACATTGGCGT ATCGATGTTC ACCTTTCCCA AGCATCATTT TGACGCCGGT 
TTCATTATAA CAGCTTTTAT TTGCGCTGCC ATTGGAAGGG CGGTGAATGT GTATCCCTTG 
TCCTGGCTGC TGAACATAAA GCGAAAGCCG AAAATTTCCA CAAACTTTCA GCATATGTTG 
TTCTTTGCTG GACTTCGTGG CGCCATGTCC TTTGCCTTGG CCATCCGAAA TACAGTGTCG 
GACGAACGAC AGACGATGCT GACTGCCACA TCGCTGATTG TCATCTTTAC GGTTGTAATT 
CAGGGTGGGG CTGCAAATTT CCTGCTTAAT TGGTTGAAAA TACCCGTTGG CGTGGACGAT 
GAGACTGAAC AACTCAATAA CTATCAAGTG CACAGTGTTT ACAATTCAAT GGACAATTCA 
CATCCACAGA CGTCTGATGG TTATTTGCAG GATGTGGAGG GCGGTGGCGT TAATCGCAAC 
AAGATGCGCT TGTCCGGTGG AACAGATACG AACTTGGACA CGCCCGTCGA TGGTACGAAT 
GGCAGTTTGG GCGGAGCAAG CGGCGGTCGC CGGCGCAACA GCCACGAGAA GGCCATTCTT 
GCTAGGATCT GGGGAAACTT TGATACCAAG TACATGAAGC CCTTACTGAC GCACTCGCGA 
CCCACTTTGC TGGAGACGCT GCCCGTTTGC TGCAATCCCA TTGCCAGACT GCTCACCACC 
ACACAGCAAC TAACGCAGGA TGGAAGCGAG TTCAGACGCG TGGACTCGGA CTCGGACATT 
TGCATAGACA ATGATACCGG GAACGGTCTC AGTCAGGATG GGTCCGGAGC GGCACCAGGC 
GTGGGGCGTC GGAACTCCCT GAGTCGCTCT GTTCACTTGA ACGAAGCCAA TCACCTTGCA 
AACGAACTCA GCCTGGTTGC TAACAATTTC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723202.2
CDS	410..2545
Translation	MLLIVGFALCVLAPGCSATDTDIALDAKATLNHRIQSLDLLVFVFLLALTVLTIWLFKHHRVSWLHETGLAVIYGLIVGAIIRYAGTSATLVHMQVEPQGVPTYSDKLPPDTLWFRYPVNQTNGTKPPEGIKTYAYVFRGQVHDVDENEIDLKATFDPEVFFNIILPPIIFYAGYSLKKKYFFRNLGAILTFAIVGTTLSAFLIGGFMYGCVKLMPKYLSSSFTFLDSLYFGALISPTDPLTILAIFNDLRVDVNLYALVLGESVLNDAVAIVLSGAIQNYGEHYSNTGEFETTAFLRSLSDFFSIFLLSLMIGAAMGCLTALMTKFTRVRDFPLLESALFVLMSYSTFLLAEATELTGVVAVLFCGICQAHYTYNNLSEDSRQRTKQIFELLNFLAENFIFSYIGVSMFTFPKHHFDAGFIITAFICAAIGRAVNVYPLSWLLNIKRKPKISTNFQHMLFFAGLRGAMSFALAIRNTVSDERQTMLTATSLIVIFTVVIQGGAANFLLNWLKIPVGVDDETEQLNNYQVHSVYNSMDNSHPQTSDGYLQDVEGGGVNRNKMRLSGGTDTNLDTPVDGTNGSLGGASGGRRRNSHEKAILARIWGNFDTKYMKPLLTHSRPTLLETLPVCCNPIARLLTTTQQLTQDGSEFRRVDSDSDICIDNDTGNGLSQDGSGAAPGVGRRNSLSRSVHLNEANHLANELSLVANNFM

Target ORF information:

RefSeq Version	NM_164701.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Na[+]/H[+] hydrogen exchanger 3 (Nhe3), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164701.2

ORF Insert Sequence:

ATGCTGCTCA TAGTCGGATT TGCATTGTGC GTCCTCGCGC CTGGATGCAG CGCCACGGAC 
ACTGACATCG CTCTGGACGC CAAGGCCACG CTCAATCACC GCATCCAGAG CCTGGACCTT 
CTAGTCTTCG TGTTCCTGCT GGCGCTCACA GTGCTCACAA TTTGGCTTTT CAAACACCAT 
CGCGTCTCCT GGCTCCACGA GACGGGATTG GCCGTCATCT ACGGCTTGAT TGTGGGTGCA 
ATTATACGAT ATGCCGGCAC CTCCGCCACC CTGGTCCACA TGCAGGTGGA GCCTCAGGGA 
GTTCCAACGT ACAGCGATAA ATTGCCGCCT GATACTCTTT GGTTTAGGTA CCCCGTTAAT 
CAAACAAATG GCACAAAGCC GCCAGAGGGA ATCAAGACGT ATGCCTACGT GTTTCGAGGA 
CAGGTACACG ATGTGGATGA GAACGAAATC GATCTTAAGG CAACGTTCGA TCCGGAGGTA 
TTTTTCAATA TTATCCTGCC CCCAATTATC TTTTATGCGG GCTATAGCTT GAAAAAGAAA 
TACTTCTTTC GCAACTTGGG TGCCATACTT ACGTTTGCCA TTGTGGGCAC GACTTTGTCG 
GCCTTCCTAA TCGGAGGCTT CATGTACGGC TGTGTGAAAC TGATGCCAAA GTACTTGAGT 
AGCAGTTTCA CATTCCTGGA CTCCTTATAC TTTGGAGCTC TGATATCGCC CACAGATCCG 
CTCACCATTC TAGCCATATT CAACGATCTT AGGGTCGACG TAAACCTATA TGCGCTAGTC 
TTGGGCGAAT CTGTGCTCAA CGATGCCGTG GCCATAGTCC TTAGTGGAGC CATTCAAAAT 
TATGGTGAAC ACTATTCCAA TACCGGGGAA TTCGAAACTA CAGCTTTTCT ACGCTCGTTG 
AGCGATTTCT TCTCCATCTT CTTACTATCC CTGATGATTG GCGCGGCCAT GGGATGCTTG 
ACAGCATTGA TGACCAAATT TACTCGGGTT CGAGACTTTC CTTTGCTAGA ATCCGCGCTC 
TTCGTGCTCA TGAGCTACAG CACCTTCCTG CTGGCTGAGG CTACGGAACT TACTGGTGTG 
GTGGCCGTGC TCTTCTGCGG CATCTGCCAG GCTCACTACA CGTACAACAA CTTGTCGGAG 
GACTCGCGCC AAAGGACAAA ACAGATATTC GAGCTCCTGA ACTTTCTAGC CGAGAATTTT 
ATCTTTTCCT ACATTGGCGT ATCGATGTTC ACCTTTCCCA AGCATCATTT TGACGCCGGT 
TTCATTATAA CAGCTTTTAT TTGCGCTGCC ATTGGAAGGG CGGTGAATGT GTATCCCTTG 
TCCTGGCTGC TGAACATAAA GCGAAAGCCG AAAATTTCCA CAAACTTTCA GCATATGTTG 
TTCTTTGCTG GACTTCGTGG CGCCATGTCC TTTGCCTTGG CCATCCGAAA TACAGTGTCG 
GACGAACGAC AGACGATGCT GACTGCCACA TCGCTGATTG TCATCTTTAC GGTTGTAATT 
CAGGGTGGGG CTGCAAATTT CCTGCTTAAT TGGTTGAAAA TACCCGTTGG CGTGGACGAT 
GAGACTGAAC AACTCAATAA CTATCAAGTG CACAGTGTTT ACAATTCAAT GGACAATTCA 
CATCCACAGA CGTCTGATGG TTATTTGCAG GATGTGGAGG GCGGTGGCGT TAATCGCAAC 
AAGATGCGCT TGTCCGGTGG AACAGATACG AACTTGGACA CGCCCGTCGA TGGTACGAAT 
GGCAGTTTGG GCGGAGCAAG CGGCGGTCGC CGGCGCAACA GCCACGAGAA GGCCATTCTT 
GCTAGGATCT GGGGAAACTT TGATACCAAG TACATGAAGC CCTTACTGAC GCACTCGCGA 
CCCACTTTGC TGGAGACGCT GCCCGTTTGC TGCAATCCCA TTGCCAGACT GCTCACCACC 
ACACAGCAAC TAACGCAGGA TGGAAGCGAG TTCAGACGCG TGGACTCGGA CTCGGACATT 
TGCATAGACA ATGATACCGG GAACGGTCTC AGTCAGGATG GGTCCGGAGC GGCACCAGGC 
GTGGGGCGTC GGAACTCCCT GAGTCGCTCT GTTCACTTGA ACGAAGCCAA TCACCTTGCA 
AACGAACTCA GCCTGGTTGC TAACAATTTC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nhe3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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