Nepl6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nepl6
Entrez Gene ID	33893
Full Name	CG42370 gene product from transcript CG42370-RA
Synonyms	CG13981,CG33295,CG9508,CG9511,CT26920,Dmel\CG42370,Dmel_CG9508,Dmel_CG9511
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26C4-26D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_135179.4	NP_609023.3	Neprilysin-like 6

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG4580	NP_609795.2
Drosophila melanogaster (fruit fly)	CG9505	NP_609019.1
Drosophila melanogaster (fruit fly)	CG9507	NP_609022.1
Drosophila melanogaster (fruit fly)	CG31918	NP_608904.1
Drosophila melanogaster (fruit fly)	CG8550	NP_610787.1
Drosophila melanogaster (fruit fly)	CG42370	NP_609023.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nepl6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nepl6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13904	NM_135179.4 Latest version!	Drosophila melanogaster Neprilysin-like 6 (Nepl6), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nepl6 ( NM_135179.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609023.3 Drosophila melanogaster Neprilysin-like 6 (Nepl6), partial mRNA.

Price & Availability↑

CloneID	OFa13904
Clone ID Related Accession (Same CDS sequence)	NM_135179.4
Accession Version	NM_135179.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neprilysin-like 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135179.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGTGGTTGA TTGCCTGCCT GTTTATTGTT CCTATTTGCG CTGCTCCAAC AACCCAGAAT 
GTCGAGGCGG ATCCCAATTC ACGGCTAATG AACAACATCC TCAGCTACGT GGCGGAGGAT 
TCCAGTGCGT GCGGCACCTA CTTTCAATAT GCCTGCGGCA AGTACGCAGT ACGTCACCGC 
GACGATCCCT TCACGGAGAT CATCCAGATG CTGGACCACA AGGTGAACGG AAACCTAGTC 
CAACTGATGC ACGAGCTGCA CCAGCGCTCC CAGTCACCCG GCTTCAACGA GTCCAGTGTG 
GAGGCCAAGG TCTTGCACTT CTACCGCACC TGCCGTGAAG CTCCGCCGAG TACACGCAAG 
ATGGAGCACT ACCTTAGACT AGCCCCTCCT GGCCAGGCAC TTACATGGCC CCAGTTTATG 
CCCCGCGGCG AGCCATGGCC AAAGGCTCAA TTCAAGTGGA TGAAGTCCTT GGGACACCTA 
TACCGCTATG GGTTTACCAA TGTTTTGGTG AACGTGTTGG TCTTACGGAG CTCTGAGAAC 
AGTAACCGCT TCCGACTGGA CATCAGTATG CCTAGTCTCG AAGGGGACGC GCAGCACCTG 
GGCGGTTTTC TAAGTACCCT CGCCACTCTC CAGATCATTG GTGTGCCGAC GAAAAGCATA 
TTACCCCTTG TGCGGAAGAT CAGAAGGCTA GAGTCCGCCA TACGCGTCCT AACCGAAGTG 
GACGACGATG ATGAAAGCGA GACTTGGACT TTGCGCCAGT GGGAGTACAA AACCGGCTAC 
GCATGGCAGG GTTTCGTCGA GTCAATAGTT GGCCATAGTG TCTCACATCA CTACGTGATG 
CAGGCGCAGA ATGCAAAGTA CTTCACGGGC CTTAAGCGGC TGATGGACTC CACGGACGCC 
GAGGTGGTGG CCAACTACAT CATGACCCGA TTCGTGCTCT ACCTCATAGA CGACTTTATT 
GACAGCAATG AGCACATTGA TTGCGTCACG GATATGCGCC GCAACATGAA TCTGGCATCG 
AACCTGCTCT ACAAGGAGCG CTTCCTGGAC CCTGCGACAC TACAGCAGTA CACCCAGGAA 
GTTACGAAGG TCTTTGAACA GCTGCGCCGG CAGTTTCTAC TGCAAATCAA TGAGAATCGC 
CTTGGGCTAA CCAGTGAGCA GAACAGTATG GTTGCCACAA AAGCTCAGCA TGTCGTTCTC 
AATATTGGGA ATTTGCCAAG AGGTCGTGAC CATCGTAGCT TTGTCAGCCG GCACTATGAG 
GATCTTGTCT TTCCGCTCCC TGATTTCGAC TACGCACGTG AACATCTTAA CCTGCTGGAG 
TTCCGCACCA GAAAGCAGGT TTTACAACTT AACCAAAGTG CTCCGACACC CGATGAGTTC 
TTCTACTTGT CGGATCCTAA AACGGCCATG AGCTCCGGAC CCTACTACCT AATGCGACTA 
AACATCATCG TTGTGCCACA CGGACTTCTA CAGGAGCCAT TCTTTGCAGC GGATAGCCAC 
GACATATTCA AGTACAGCCT GTTGGGATTC GTACTTGCCC ATGAGTTGAT GCACTCAGTC 
GACACCGTGG GTCTTTATTA TGACAGATAT GGGAACATAC ACGAAATCGG ACCGCAGATA 
TCCAGTTCGC CACGCTTTGT GGCGGGTTTA CAGTGCCTGA ACCGGAACAG TACACAGTAT 
CTGGACGAGC GTATTGCAGA CATTGCTGGA CTGGATCTGG CCTATTCCAC CTACTTCCAG 
AACAGCTCGC AAATGAGTCT AACCGATTTT ACGACCATTC CGCCGCAACA AATCTTCTTC 
CTCAATCTGG CACAGTTCTT CTGCGGCGAT GGTGATCCAA TAAACTTCGT TGGTCACGAC 
AACGACGAGA CGCGTCTGCA GCAAATGCTC AATGGGTTCG CGCCGTTTCA CGAAGCTTTT 
GGTTGTCCAG CAAAGAGCAA TCAGCCTGAA AAGTGCCAAC TGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609023.3
CDS	1..1968
Translation	MWLIACLFIVPICAAPTTQNVEADPNSRLMNNILSYVAEDSSACGTYFQYACGKYAVRHRDDPFTEIIQMLDHKVNGNLVQLMHELHQRSQSPGFNESSVEAKVLHFYRTCREAPPSTRKMEHYLRLAPPGQALTWPQFMPRGEPWPKAQFKWMKSLGHLYRYGFTNVLVNVLVLRSSENSNRFRLDISMPSLEGDAQHLGGFLSTLATLQIIGVPTKSILPLVRKIRRLESAIRVLTEVDDDDESETWTLRQWEYKTGYAWQGFVESIVGHSVSHHYVMQAQNAKYFTGLKRLMDSTDAEVVANYIMTRFVLYLIDDFIDSNEHIDCVTDMRRNMNLASNLLYKERFLDPATLQQYTQEVTKVFEQLRRQFLLQINENRLGLTSEQNSMVATKAQHVVLNIGNLPRGRDHRSFVSRHYEDLVFPLPDFDYAREHLNLLEFRTRKQVLQLNQSAPTPDEFFYLSDPKTAMSSGPYYLMRLNIIVVPHGLLQEPFFAADSHDIFKYSLLGFVLAHELMHSVDTVGLYYDRYGNIHEIGPQISSSPRFVAGLQCLNRNSTQYLDERIADIAGLDLAYSTYFQNSSQMSLTDFTTIPPQQIFFLNLAQFFCGDGDPINFVGHDNDETRLQQMLNGFAPFHEAFGCPAKSNQPEKCQLW

Target ORF information:

RefSeq Version	NM_135179.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neprilysin-like 6 (Nepl6), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135179.4

ORF Insert Sequence:

ATGTGGTTGA TTGCCTGCCT GTTTATTGTT CCTATTTGCG CTGCTCCAAC AACCCAGAAT 
GTCGAGGCGG ATCCCAATTC ACGGCTAATG AACAACATCC TCAGCTACGT GGCGGAGGAT 
TCCAGTGCGT GCGGCACCTA CTTTCAATAT GCCTGCGGCA AGTACGCAGT ACGTCACCGC 
GACGATCCCT TCACGGAGAT CATCCAGATG CTGGACCACA AGGTGAACGG AAACCTAGTC 
CAACTGATGC ACGAGCTGCA CCAGCGCTCC CAGTCACCCG GCTTCAACGA GTCCAGTGTG 
GAGGCCAAGG TCTTGCACTT CTACCGCACC TGCCGTGAAG CTCCGCCGAG TACACGCAAG 
ATGGAGCACT ACCTTAGACT AGCCCCTCCT GGCCAGGCAC TTACATGGCC CCAGTTTATG 
CCCCGCGGCG AGCCATGGCC AAAGGCTCAA TTCAAGTGGA TGAAGTCCTT GGGACACCTA 
TACCGCTATG GGTTTACCAA TGTTTTGGTG AACGTGTTGG TCTTACGGAG CTCTGAGAAC 
AGTAACCGCT TCCGACTGGA CATCAGTATG CCTAGTCTCG AAGGGGACGC GCAGCACCTG 
GGCGGTTTTC TAAGTACCCT CGCCACTCTC CAGATCATTG GTGTGCCGAC GAAAAGCATA 
TTACCCCTTG TGCGGAAGAT CAGAAGGCTA GAGTCCGCCA TACGCGTCCT AACCGAAGTG 
GACGACGATG ATGAAAGCGA GACTTGGACT TTGCGCCAGT GGGAGTACAA AACCGGCTAC 
GCATGGCAGG GTTTCGTCGA GTCAATAGTT GGCCATAGTG TCTCACATCA CTACGTGATG 
CAGGCGCAGA ATGCAAAGTA CTTCACGGGC CTTAAGCGGC TGATGGACTC CACGGACGCC 
GAGGTGGTGG CCAACTACAT CATGACCCGA TTCGTGCTCT ACCTCATAGA CGACTTTATT 
GACAGCAATG AGCACATTGA TTGCGTCACG GATATGCGCC GCAACATGAA TCTGGCATCG 
AACCTGCTCT ACAAGGAGCG CTTCCTGGAC CCTGCGACAC TACAGCAGTA CACCCAGGAA 
GTTACGAAGG TCTTTGAACA GCTGCGCCGG CAGTTTCTAC TGCAAATCAA TGAGAATCGC 
CTTGGGCTAA CCAGTGAGCA GAACAGTATG GTTGCCACAA AAGCTCAGCA TGTCGTTCTC 
AATATTGGGA ATTTGCCAAG AGGTCGTGAC CATCGTAGCT TTGTCAGCCG GCACTATGAG 
GATCTTGTCT TTCCGCTCCC TGATTTCGAC TACGCACGTG AACATCTTAA CCTGCTGGAG 
TTCCGCACCA GAAAGCAGGT TTTACAACTT AACCAAAGTG CTCCGACACC CGATGAGTTC 
TTCTACTTGT CGGATCCTAA AACGGCCATG AGCTCCGGAC CCTACTACCT AATGCGACTA 
AACATCATCG TTGTGCCACA CGGACTTCTA CAGGAGCCAT TCTTTGCAGC GGATAGCCAC 
GACATATTCA AGTACAGCCT GTTGGGATTC GTACTTGCCC ATGAGTTGAT GCACTCAGTC 
GACACCGTGG GTCTTTATTA TGACAGATAT GGGAACATAC ACGAAATCGG ACCGCAGATA 
TCCAGTTCGC CACGCTTTGT GGCGGGTTTA CAGTGCCTGA ACCGGAACAG TACACAGTAT 
CTGGACGAGC GTATTGCAGA CATTGCTGGA CTGGATCTGG CCTATTCCAC CTACTTCCAG 
AACAGCTCGC AAATGAGTCT AACCGATTTT ACGACCATTC CGCCGCAACA AATCTTCTTC 
CTCAATCTGG CACAGTTCTT CTGCGGCGAT GGTGATCCAA TAAACTTCGT TGGTCACGAC 
AACGACGAGA CGCGTCTGCA GCAAATGCTC AATGGGTTCG CGCCGTTTCA CGAAGCTTTT 
GGTTGTCCAG CAAAGAGCAA TCAGCCTGAA AAGTGCCAAC TGTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nepl6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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