Nepl5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nepl5
Entrez Gene ID	33892
Full Name	CG9507 gene product from transcript CG9507-RA
Synonyms	Dmel\CG9507
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26C4-26C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_135178.3	NP_609022.1	Neprilysin-like 5

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG4580	NP_609795.2
Drosophila melanogaster (fruit fly)	CG9505	NP_609019.1
Drosophila melanogaster (fruit fly)	CG9507	NP_609022.1
Drosophila melanogaster (fruit fly)	CG31918	NP_608904.1
Drosophila melanogaster (fruit fly)	CG8550	NP_610787.1
Drosophila melanogaster (fruit fly)	CG42370	NP_609023.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nepl5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nepl5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13903	NM_135178.3 Latest version!	Drosophila melanogaster Neprilysin-like 5 (Nepl5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nepl5 ( NM_135178.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609022.1 Drosophila melanogaster Neprilysin-like 5 (Nepl5), mRNA.

Price & Availability↑

CloneID	OFa13903
Clone ID Related Accession (Same CDS sequence)	NM_135178.3
Accession Version	NM_135178.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neprilysin-like 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135178.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAAGC TAAGCCTAGT GGCCTGGCTC ATATTTTCGC TGGCTTGGCG CACCACGCCC 
ACCGCCTGTC GCCAGACCAA CGCACCCCAG AGCGTTCAGG AAATGCTGGC CGAGCAGCTG 
CAAAGCTACA TGGATACGAA GGCGCGTCCC TGCGAGAACT TCTACCAGTA CGCCTGCGGC 
AACTGGCAGA TCCAGCAGCA GGAGCAGCAC TCCCTGCGCG ACAGAGATTG GACGAGGGAT 
CGGCAGCGGT ACCAGGGTCA GATGCTGCCC ACCGACACCC TGGGACTGAT AGACCACTCC 
GTGAATCGGA AGCTGGAGCT GCTGCTGCGG CGAAGGAATG AGAGCTCCTT CGAGTCGGAC 
TCCTCGATTC TGGAGCAGAT GCGACTCTAC TACCGTTCGT GCAAACGCCT TAAGCCGTAC 
AACCTCAAGA AGTACCTGCA GCTCCTGCCC CCCAGCAACT CCACGCAGTG GCCATCGGTG 
GGTCGGGGCT GGCGTCCGGA GCGGTTCGAC TGGATAACGA CCTTGGGGCG ACTGCGTCTG 
CACGGACTGA ACGGGGTGCT GCTGCGGGAG GAGGTACTGC CGCGATGGGA TGACTCTCGC 
AACTACAGTA TCTATGTGAA CAAGCCGAGT CGCCAGGAGA CGCAGCCCAT GGGAGAAGGT 
GCCATGATCG AGCTGCTTCT GGACATTGGG CAGACAAAGC GAACGGCCAA TGCCTTGGCT 
CGCCAGGTGG ACGACTTCGA ACGAAAATTG CACCGCCTGC AGGAGCTGGA GGACGACGAA 
GGACCACGAG AAATGCAGCT TGGTTACTTG GACTCGTACC TGCCACAGCT ACGATGGTTA 
TCCTTCATGC GCCAGGTACG CATTGACTCC GAGCTGGATC TGCGCTCCAC ATTAATCATC 
GAGAATATTC CGTACCTCAG GGCCCTGAGC GATCTGATGG AATCGGAGAG CCCGGACACC 
GTTTGCAGCT ACATCATGCT CAAGTGGTTG GCCTTCCTCA AGCAACAGGG ACCCGCGGAC 
ATCTCACGAG GTGAGTGCGT GGCCGCCCTC AGAAGAGCCA TGCCTCTGGC CAGTAGCTGG 
CTGGTGGGTC AGCAGTTCTC CGATCCGGAA TCCGAATCCT ACGTAAGCTC ACTGTTCCAG 
CGTTTGAAGG TGCGATTTGG CCAAATCTTG GCTGAGAACC GAATGAGACT ATCGCCACCA 
CTGGTGCACA TCCTGCAGCA GAAACTGCGC GCAGTTCGAC TGCAGATGGG CTTCTTTCAG 
ACGGAGGAAA TTGAGGATGT GGAGCAGTAC CACGTCCGCA TCGACCTGAG CGGCCACTCC 
TTCTATGGCA ACCAGCTGGT CTTGCTGCGA CAACGCGTTG AGGCGAACCA CGATCTGCTT 
TACATAAATG TGACAAACTT CGCAAATTCC ACGGGGAGCA ACTTGAGTTA CCTGTCGGAA 
AGCTGGGAGG CCAGCAACTC GTCGCCCCTG TATGTGAGAC CACGCAATCT GGTCCTGGTG 
CCGCACGGTC TGCTCCAGCT GCCCATCTGG CATCGAAACA TTAGCGCCCT GCAGCAGCAC 
GCCGTGATGG GATTTGTCCT GGCCCACGAG CTGGCACACG GATTCGATAT GTTGGGAATG 
GACTACGACG CCATGGGAAA CATCATGGGG CCCGTGGAGG AGATCGGCGC TAGCCGTCAG 
TTCCGACAAG GACTCCAGTG TCTTCAGCAA CAGATGGCCA CCGGTTCCAA GTGGATCGAC 
GAAAAGCTGG CCGACTTTGT GGCTCTGCGT CTAGCGTATG AGACCTTTTT CGGGGTGCGG 
GACAAGCGGG AGCCCAGGGA TCCTCTGCTG CCGCAGTTCA GCCAGCGGCA GCTATTCTTC 
ATCACCTTCG CCCAGTTCTT TTGCGGCCGG ACGCCCGTGC TCAGTCCGCG ATCTCAGTCG 
GAGGCTCATT TGAAGCACGC CGCGGATGAG TTGCGTGTGA TGCAGACGCT GGCCAATTTT 
GAAGAGTTCT CTAGGGAGTT CGGGTGCGAT AAGAAGGCCA AGATGCAGGC CAGCCAGCGG 
TGCCGCATCT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609022.1
CDS	33..2087
Translation	MLKLSLVAWLIFSLAWRTTPTACRQTNAPQSVQEMLAEQLQSYMDTKARPCENFYQYACGNWQIQQQEQHSLRDRDWTRDRQRYQGQMLPTDTLGLIDHSVNRKLELLLRRRNESSFESDSSILEQMRLYYRSCKRLKPYNLKKYLQLLPPSNSTQWPSVGRGWRPERFDWITTLGRLRLHGLNGVLLREEVLPRWDDSRNYSIYVNKPSRQETQPMGEGAMIELLLDIGQTKRTANALARQVDDFERKLHRLQELEDDEGPREMQLGYLDSYLPQLRWLSFMRQVRIDSELDLRSTLIIENIPYLRALSDLMESESPDTVCSYIMLKWLAFLKQQGPADISRGECVAALRRAMPLASSWLVGQQFSDPESESYVSSLFQRLKVRFGQILAENRMRLSPPLVHILQQKLRAVRLQMGFFQTEEIEDVEQYHVRIDLSGHSFYGNQLVLLRQRVEANHDLLYINVTNFANSTGSNLSYLSESWEASNSSPLYVRPRNLVLVPHGLLQLPIWHRNISALQQHAVMGFVLAHELAHGFDMLGMDYDAMGNIMGPVEEIGASRQFRQGLQCLQQQMATGSKWIDEKLADFVALRLAYETFFGVRDKREPRDPLLPQFSQRQLFFITFAQFFCGRTPVLSPRSQSEAHLKHAADELRVMQTLANFEEFSREFGCDKKAKMQASQRCRIW

Target ORF information:

RefSeq Version	NM_135178.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neprilysin-like 5 (Nepl5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135178.3

ORF Insert Sequence:

ATGCTGAAGC TAAGCCTAGT GGCCTGGCTC ATATTTTCGC TGGCTTGGCG CACCACGCCC 
ACCGCCTGTC GCCAGACCAA CGCACCCCAG AGCGTTCAGG AAATGCTGGC CGAGCAGCTG 
CAAAGCTACA TGGATACGAA GGCGCGTCCC TGCGAGAACT TCTACCAGTA CGCCTGCGGC 
AACTGGCAGA TCCAGCAGCA GGAGCAGCAC TCCCTGCGCG ACAGAGATTG GACGAGGGAT 
CGGCAGCGGT ACCAGGGTCA GATGCTGCCC ACCGACACCC TGGGACTGAT AGACCACTCC 
GTGAATCGGA AGCTGGAGCT GCTGCTGCGG CGAAGGAATG AGAGCTCCTT CGAGTCGGAC 
TCCTCGATTC TGGAGCAGAT GCGACTCTAC TACCGTTCGT GCAAACGCCT TAAGCCGTAC 
AACCTCAAGA AGTACCTGCA GCTCCTGCCC CCCAGCAACT CCACGCAGTG GCCATCGGTG 
GGTCGGGGCT GGCGTCCGGA GCGGTTCGAC TGGATAACGA CCTTGGGGCG ACTGCGTCTG 
CACGGACTGA ACGGGGTGCT GCTGCGGGAG GAGGTACTGC CGCGATGGGA TGACTCTCGC 
AACTACAGTA TCTATGTGAA CAAGCCGAGT CGCCAGGAGA CGCAGCCCAT GGGAGAAGGT 
GCCATGATCG AGCTGCTTCT GGACATTGGG CAGACAAAGC GAACGGCCAA TGCCTTGGCT 
CGCCAGGTGG ACGACTTCGA ACGAAAATTG CACCGCCTGC AGGAGCTGGA GGACGACGAA 
GGACCACGAG AAATGCAGCT TGGTTACTTG GACTCGTACC TGCCACAGCT ACGATGGTTA 
TCCTTCATGC GCCAGGTACG CATTGACTCC GAGCTGGATC TGCGCTCCAC ATTAATCATC 
GAGAATATTC CGTACCTCAG GGCCCTGAGC GATCTGATGG AATCGGAGAG CCCGGACACC 
GTTTGCAGCT ACATCATGCT CAAGTGGTTG GCCTTCCTCA AGCAACAGGG ACCCGCGGAC 
ATCTCACGAG GTGAGTGCGT GGCCGCCCTC AGAAGAGCCA TGCCTCTGGC CAGTAGCTGG 
CTGGTGGGTC AGCAGTTCTC CGATCCGGAA TCCGAATCCT ACGTAAGCTC ACTGTTCCAG 
CGTTTGAAGG TGCGATTTGG CCAAATCTTG GCTGAGAACC GAATGAGACT ATCGCCACCA 
CTGGTGCACA TCCTGCAGCA GAAACTGCGC GCAGTTCGAC TGCAGATGGG CTTCTTTCAG 
ACGGAGGAAA TTGAGGATGT GGAGCAGTAC CACGTCCGCA TCGACCTGAG CGGCCACTCC 
TTCTATGGCA ACCAGCTGGT CTTGCTGCGA CAACGCGTTG AGGCGAACCA CGATCTGCTT 
TACATAAATG TGACAAACTT CGCAAATTCC ACGGGGAGCA ACTTGAGTTA CCTGTCGGAA 
AGCTGGGAGG CCAGCAACTC GTCGCCCCTG TATGTGAGAC CACGCAATCT GGTCCTGGTG 
CCGCACGGTC TGCTCCAGCT GCCCATCTGG CATCGAAACA TTAGCGCCCT GCAGCAGCAC 
GCCGTGATGG GATTTGTCCT GGCCCACGAG CTGGCACACG GATTCGATAT GTTGGGAATG 
GACTACGACG CCATGGGAAA CATCATGGGG CCCGTGGAGG AGATCGGCGC TAGCCGTCAG 
TTCCGACAAG GACTCCAGTG TCTTCAGCAA CAGATGGCCA CCGGTTCCAA GTGGATCGAC 
GAAAAGCTGG CCGACTTTGT GGCTCTGCGT CTAGCGTATG AGACCTTTTT CGGGGTGCGG 
GACAAGCGGG AGCCCAGGGA TCCTCTGCTG CCGCAGTTCA GCCAGCGGCA GCTATTCTTC 
ATCACCTTCG CCCAGTTCTT TTGCGGCCGG ACGCCCGTGC TCAGTCCGCG ATCTCAGTCG 
GAGGCTCATT TGAAGCACGC CGCGGATGAG TTGCGTGTGA TGCAGACGCT GGCCAATTTT 
GAAGAGTTCT CTAGGGAGTT CGGGTGCGAT AAGAAGGCCA AGATGCAGGC CAGCCAGCGG 
TGCCGCATCT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nepl5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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