Nepl4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nepl4
Entrez Gene ID	33889
Full Name	CG9505 gene product from transcript CG9505-RA
Synonyms	Dmel\CG9505
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26C4-26C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_135175.3	NP_609019.1	Neprilysin-like 4

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG4580	NP_609795.2
Drosophila melanogaster (fruit fly)	CG9505	NP_609019.1
Drosophila melanogaster (fruit fly)	CG9507	NP_609022.1
Drosophila melanogaster (fruit fly)	CG31918	NP_608904.1
Drosophila melanogaster (fruit fly)	CG8550	NP_610787.1
Drosophila melanogaster (fruit fly)	CG42370	NP_609023.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nepl4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nepl4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13901	NM_135175.3 Latest version!	Drosophila melanogaster Neprilysin-like 4 (Nepl4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nepl4 ( NM_135175.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609019.1 Drosophila melanogaster Neprilysin-like 4 (Nepl4), mRNA.

Price & Availability↑

CloneID	OFa13901
Clone ID Related Accession (Same CDS sequence)	NM_135175.3
Accession Version	NM_135175.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neprilysin-like 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135175.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGCAAA ATCTGTGGAC TGCCCTCCTT CTGCTGGGCA GTGCCACCTG CGGTTCGAGT 
GTGCCGGTCA AGGCAAATGA TCCGGAACAG AGTTGTCCGT ACCTCGGAGA GTGCAATTCC 
ACGATCAATC AGCAGCACAT CGATACGCTG ATGTCCTACG TGGACAGCGA AAAAAATCCC 
TGCGAGGATT TCTATGCCTA CGCCTGCGGA AAATGGCGGG CGAAGCATGG AAGCCACACA 
ACCGCCACGA TGATCAGCGA ATCGCAAATT AACCGCCAGT ACGAGGATTT GTTCCTACAA 
CTGCTGAGGA ATCCCAGCGC CCCGGAATAT GGATACCCCA TGTTCGCCAA GGTGTTGGCC 
CATTACCAGT CATGCATCGC GCTGGAGAAG CCGGATCTGA GGCGATACAT CGAGCTACTG 
GACCGGGATC TGCTCGCCTC CTTAAACTCC ACACACTGGA TGCACCTGCT GGCAGCTCTG 
GGGCGCTACG GCTACCACGG TCACTACGTC CAAGTAGAGG TACGTTGGTA CAACGCCACC 
CATCACATGA TCTTCCTGCT GCCGCACAAT CGCCACCTAA ACCTGAGTCT CACGCAAGAC 
ATCTATGATG CGCTGAGTCA GGATGGCTCC TCCTGGCCAC CGCTCCACCA GCTACAGGAG 
CAGTTCAGAA GTTTGGAGCA GAACTTGGTG CGACTGGCTA AGCCCCATTC GGCGGACGAT 
ACCTTCCGGA ATTACAGTCT GGATCAGATT CGGACGGAGG TGCCGGGTCT GCATTGGGAC 
GAGGCGCTGA GGACACAGCT GGGCAGATCG GTGCCGGGGA ATCATGTCTT CCAGGTGGAC 
GACTTGGACG CGATCGAGGG GCTTGTGGAG TACCTCAATA CGGTAGATAC TCTGCTGCTC 
AATCGGTACA GCCTGGCAAG ATTTCTCAGC CACCTGCTTG AGCTGCCACA TAACCCGCTG 
GCCACTTGGG AGTCCGGACA GCGGTCGCGG GGAAGGAACT GTATTCGTCA CATGCGCCGC 
TCTGTTTACC TGCCGATGAA CTACGTCTAC GAGAGAAGTT TCTACTCCCG GCGCCGCCAC 
GCGGATGAGC TGGTCATCCA CAGCGTCTTC CAGCAGCTGC AGAGTCAGCT GGAGCTGCGG 
GTCCAGCACA ACGCCTTCAA TCTAAGTCAG GATCTGGTGA AATCGCTGCA GGCAAAGGTG 
CACCAGATGC GCATCAATGT GGGCAACCTA CCGCCCAATG TGACGGAGCA ATTCTATTGG 
GACAGCGACC GGCGGTGGAG CGTGGGCAGG GACTTCTACG AGAATCACCT GAACAGCCTG 
CTCTACTACT ACACGCTCGT CGCCGATTTG GAAAGCAGCT CGGACCAGGA GGAGCGGGAC 
ATCTGGTACA GCTTCAATAT GCATACGCCC GAGTTTCCCG ACAACATCGA TGCCACGCCG 
TACTTCTACT GCCTGGGCAA CATCATCTTC GTGCCGTACT CCTACGTAAA GCGTCCCTTC 
TTCGATGCCA ACTTCTGGCC AGCGCTGCTC TACGGTGATC TGGCCAACAC CCTGGGCCAC 
GAGATCATGC ACGCCTTCGA CACCGATCTG GTGGACTACG ATGCGCAGGG TAATCTGCGT 
AACTTTAGCG ACCAGTTGGG CGAAGTGGAG CTCTACAACG GAGCGGTTGG ATGCCTGAAC 
AGCAGCGCTG TTATGCTAAA CGAGCGCACC TCCGACGTCA GCGGATCCCG GCTGGCCATG 
CAAACCTACG GTGGCGACTG GCTCACTCGG TCGGGCAACG GGCGTCTGTA CTTCCTCCAG 
TTCGCCCACT TCTTCTGCGG CGACGAGGGC GACCAGTACC ATGACTCCGG CAGCCAGCGG 
CTGAACTACG CCCTCGGCCA GGTGCCGCAG TTCGCAGAGG TGTTCCGGTG CCATGTTGGC 
TCCGGAATGA CCAGCGCGGA CCAGTGTCCC TTCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609019.1
CDS	260..2218
Translation	MVQNLWTALLLLGSATCGSSVPVKANDPEQSCPYLGECNSTINQQHIDTLMSYVDSEKNPCEDFYAYACGKWRAKHGSHTTATMISESQINRQYEDLFLQLLRNPSAPEYGYPMFAKVLAHYQSCIALEKPDLRRYIELLDRDLLASLNSTHWMHLLAALGRYGYHGHYVQVEVRWYNATHHMIFLLPHNRHLNLSLTQDIYDALSQDGSSWPPLHQLQEQFRSLEQNLVRLAKPHSADDTFRNYSLDQIRTEVPGLHWDEALRTQLGRSVPGNHVFQVDDLDAIEGLVEYLNTVDTLLLNRYSLARFLSHLLELPHNPLATWESGQRSRGRNCIRHMRRSVYLPMNYVYERSFYSRRRHADELVIHSVFQQLQSQLELRVQHNAFNLSQDLVKSLQAKVHQMRINVGNLPPNVTEQFYWDSDRRWSVGRDFYENHLNSLLYYYTLVADLESSSDQEERDIWYSFNMHTPEFPDNIDATPYFYCLGNIIFVPYSYVKRPFFDANFWPALLYGDLANTLGHEIMHAFDTDLVDYDAQGNLRNFSDQLGEVELYNGAVGCLNSSAVMLNERTSDVSGSRLAMQTYGGDWLTRSGNGRLYFLQFAHFFCGDEGDQYHDSGSQRLNYALGQVPQFAEVFRCHVGSGMTSADQCPFW

Target ORF information:

RefSeq Version	NM_135175.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neprilysin-like 4 (Nepl4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135175.3

ORF Insert Sequence:

ATGGTGCAAA ATCTGTGGAC TGCCCTCCTT CTGCTGGGCA GTGCCACCTG CGGTTCGAGT 
GTGCCGGTCA AGGCAAATGA TCCGGAACAG AGTTGTCCGT ACCTCGGAGA GTGCAATTCC 
ACGATCAATC AGCAGCACAT CGATACGCTG ATGTCCTACG TGGACAGCGA AAAAAATCCC 
TGCGAGGATT TCTATGCCTA CGCCTGCGGA AAATGGCGGG CGAAGCATGG AAGCCACACA 
ACCGCCACGA TGATCAGCGA ATCGCAAATT AACCGCCAGT ACGAGGATTT GTTCCTACAA 
CTGCTGAGGA ATCCCAGCGC CCCGGAATAT GGATACCCCA TGTTCGCCAA GGTGTTGGCC 
CATTACCAGT CATGCATCGC GCTGGAGAAG CCGGATCTGA GGCGATACAT CGAGCTACTG 
GACCGGGATC TGCTCGCCTC CTTAAACTCC ACACACTGGA TGCACCTGCT GGCAGCTCTG 
GGGCGCTACG GCTACCACGG TCACTACGTC CAAGTAGAGG TACGTTGGTA CAACGCCACC 
CATCACATGA TCTTCCTGCT GCCGCACAAT CGCCACCTAA ACCTGAGTCT CACGCAAGAC 
ATCTATGATG CGCTGAGTCA GGATGGCTCC TCCTGGCCAC CGCTCCACCA GCTACAGGAG 
CAGTTCAGAA GTTTGGAGCA GAACTTGGTG CGACTGGCTA AGCCCCATTC GGCGGACGAT 
ACCTTCCGGA ATTACAGTCT GGATCAGATT CGGACGGAGG TGCCGGGTCT GCATTGGGAC 
GAGGCGCTGA GGACACAGCT GGGCAGATCG GTGCCGGGGA ATCATGTCTT CCAGGTGGAC 
GACTTGGACG CGATCGAGGG GCTTGTGGAG TACCTCAATA CGGTAGATAC TCTGCTGCTC 
AATCGGTACA GCCTGGCAAG ATTTCTCAGC CACCTGCTTG AGCTGCCACA TAACCCGCTG 
GCCACTTGGG AGTCCGGACA GCGGTCGCGG GGAAGGAACT GTATTCGTCA CATGCGCCGC 
TCTGTTTACC TGCCGATGAA CTACGTCTAC GAGAGAAGTT TCTACTCCCG GCGCCGCCAC 
GCGGATGAGC TGGTCATCCA CAGCGTCTTC CAGCAGCTGC AGAGTCAGCT GGAGCTGCGG 
GTCCAGCACA ACGCCTTCAA TCTAAGTCAG GATCTGGTGA AATCGCTGCA GGCAAAGGTG 
CACCAGATGC GCATCAATGT GGGCAACCTA CCGCCCAATG TGACGGAGCA ATTCTATTGG 
GACAGCGACC GGCGGTGGAG CGTGGGCAGG GACTTCTACG AGAATCACCT GAACAGCCTG 
CTCTACTACT ACACGCTCGT CGCCGATTTG GAAAGCAGCT CGGACCAGGA GGAGCGGGAC 
ATCTGGTACA GCTTCAATAT GCATACGCCC GAGTTTCCCG ACAACATCGA TGCCACGCCG 
TACTTCTACT GCCTGGGCAA CATCATCTTC GTGCCGTACT CCTACGTAAA GCGTCCCTTC 
TTCGATGCCA ACTTCTGGCC AGCGCTGCTC TACGGTGATC TGGCCAACAC CCTGGGCCAC 
GAGATCATGC ACGCCTTCGA CACCGATCTG GTGGACTACG ATGCGCAGGG TAATCTGCGT 
AACTTTAGCG ACCAGTTGGG CGAAGTGGAG CTCTACAACG GAGCGGTTGG ATGCCTGAAC 
AGCAGCGCTG TTATGCTAAA CGAGCGCACC TCCGACGTCA GCGGATCCCG GCTGGCCATG 
CAAACCTACG GTGGCGACTG GCTCACTCGG TCGGGCAACG GGCGTCTGTA CTTCCTCCAG 
TTCGCCCACT TCTTCTGCGG CGACGAGGGC GACCAGTACC ATGACTCCGG CAGCCAGCGG 
CTGAACTACG CCCTCGGCCA GGTGCCGCAG TTCGCAGAGG TGTTCCGGTG CCATGTTGGC 
TCCGGAATGA CCAGCGCGGA CCAGTGTCCC TTCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nepl4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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