PDZ-GEF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDZ-GEF
Entrez Gene ID	33881
Full Name	PDZ domain-containing guanine nucleotide exchange factor
Synonyms	CG9491,D-PDZGEF,DGEF,Dmel\CG9491,Dzy,EC2-8,GEF,Gef26,PDF-GEF Dizzy,dPDZ-GEF,dizzy,dzy,l(2)k13720
General protein information	Preferred Names PDZ domain-containing guanine nucleotide exchange factor Names CG9491 gene product from transcript CG9491-RD CG9491-PA CG9491-PB CG9491-PC CG9491-PD GEF Gef26 PDZ-GEF-PA PDZ-GEF-PB PDZ-GEF-PC PDZ-GEF-PD PDZ-GEF/Dizzy dizzy
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26C2-26C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273197.1	NP_001260126.1	PDZ domain-containing guanine nucleotide exchange factor, isoform D
NM_001144325.2	NP_001137797.1	PDZ domain-containing guanine nucleotide exchange factor, isoform B
NM_001144326.2	NP_001137798.1	PDZ domain-containing guanine nucleotide exchange factor, isoform C
NM_135168.5	NP_609012.2	PDZ domain-containing guanine nucleotide exchange factor, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009261	XP_553211.3
Drosophila melanogaster (fruit fly)	Gef26	NP_001137798.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that dPDZ-GEF/Rap1/Canoe signaling modulates cell shape and apicolateral cell constriction in embryonic and wing disc epithelia.
Title: The Rap GTPase activator Drosophila PDZ-GEF regulates cell shape in epithelial migration and morphogenesis.

The PDZ-GEF Dizzy regulates cell shape of migrating macrophages via Rap1 and integrins in the Drosophila embryo.
Title: The PDZ-GEF dizzy regulates cell shape of migrating macrophages via Rap1 and integrins in the Drosophila embryo.

Rap-GEF/Rap signaling restricts the formation of supernumerary spermathecae in Drosophila melanogaster.
Title: Rap-GEF/Rap signaling restricts the formation of supernumerary spermathecae in Drosophila melanogaster.

Rap-GEF signaling controls stem cell anchoring to their niche through regulating DE-cadherin-mediated cell adhesion in the Drosophila testis.
Title: Rap-GEF signaling controls stem cell anchoring to their niche through regulating DE-cadherin-mediated cell adhesion in the Drosophila testis.

PDZ-GEF is an upstream regulator of morphogenetic pathways and is involved in Drosophila photoreceptor development
Title: Drosophila PDZ-GEF, a guanine nucleotide exchange factor for Rap1 GTPase, reveals a novel upstream regulatory mechanism in the mitogen-activated protein kinase signaling pathway.

The following PDZ-GEF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDZ-GEF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15802	NM_001144326.2 Latest version!	Drosophila melanogaster PDZ domain-containing guanine nucleotide exchange factor (PDZ-GEF), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa13892	NM_135168.5 Latest version!	Drosophila melanogaster PDZ domain-containing guanine nucleotide exchange factor (PDZ-GEF), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa15801	NM_001273197.1 Latest version!	Drosophila melanogaster PDZ domain-containing guanine nucleotide exchange factor (PDZ-GEF), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa15801	NM_001144325.2 Latest version!	Drosophila melanogaster PDZ domain-containing guanine nucleotide exchange factor (PDZ-GEF), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDZ-GEF ( NM_001144326.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137798.1 Drosophila melanogaster PDZ domain-containing guanine nucleotide exchange factor (PDZ-GEF), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa15802
Clone ID Related Accession (Same CDS sequence)	NM_001144326.2
Accession Version	NM_001144326.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PDZ domain-containing guanine nucleotide exchange factor, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001144326.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCCGT ATCACCATAT CAGACATCAT TATCCACCCA CAAGCATAGC AGGCACAGGA 
GTGGTGGTGG GCAGCTCGAC TACAATCAAC CGGCCGGAAC TGCACCAAAA ATGCAACCGG 
GGCTCGCACT CCAGTGACAC CAGTTCGGCG TACAGCGGCA GCGATACAAT GGCCTCGAAC 
TATGCCTCGT CGCTGGAGGC CGAGGAGATT GACCTCTCCG GCCTGGTGGA GTCCGTTGTG 
GACTCTGACG AGGAGGACCT GGCGGAGAGC ATGGATAGTC TTACCGTGCG GGATGCCGTG 
CGTGACTGTC TGGAAAAGGA TCCGGCGGAG CGCAGCGAGG AAGATGTGGA AGTACTGCTT 
GAGTTCACGC AGGGCCTTAA GGCATTCACA AACATTACGC TGGCAGTGCG AAGAGCACTA 
TGTTCCGTGA TGGTATTCGC CGTTGTGGAC AAGGCGGGAA CCGTGGTCAT GTCAGATGGT 
GAGGAACTGG ACTCATGGTC GGTGCTCATA AACGGAGCGG TGGAGATCGA GCATGCAAAT 
GGCAGTCGCG AAGAGCTGCA GATGGGAGAC TCCTTCGGCA TTCTACCCAC CATGGACAAG 
CTGTACCACC GGGGTGTGAT GCGTACGAAG TGCGATGACT GCCAATTTGT GTGCATTACG 
CAGACGGACT ACTACCGTAT TCAGCATCAA GGCGAGGAGA ATACCCGGCG ACATGAGGAC 
GAGAATGGTT TCGTTGTTAT GGTCACTGAA CTCCGGTCCA TTGGCGGCGC CGGCACTGAT 
TCTGCTGGCA GTGGCGGATC GGCGACAGGA GCTTCGGCTT CGCTAAATAT GAAGCGTGGT 
CACGTAGTTA TCCGCGGTAC TCCAGAGCGT CTGCTACAGC AGCTGGTCGA GGAAAACTCG 
ATGACCGATC CCACATACGT GGAGGACTTT TTGCTCACAC ACCGCATCTT TATTCAGAAC 
CCACAAGAGG TGACCAGCAA GTTGCTGCAT TGGTTTGATT TGGAGCAGGT GGATGCGCAC 
AAGACGCAGG AACTGCGAGA TCGCGTAACA CGCGTCGTGC TCCTCTGGGT GAACAACCAT 
TTCACCGATT TCGAGGCGGA CTATGAGATG ATGGAGTTTC TGGAAGTCTT TGAGGCACTG 
TTAGAGCGAA AAAAGTTGCT AAGTCAGCTG CGCCTGTTGC ACATAGCCTG TGCCGCCAAA 
GCACGGATGC GCAGCTGTAC CCTCACTCGA TCGTCACGTG ACGAGCCGCT CAACTTCCGC 
ATCGTGGGCG GCTACGAGCT TCGAGGCGTC GCCATAGCCA CTGGAAACGC AGCAGTGGGC 
ATCTACATTT CGCACGTCGA GCCGGGATCA AAAGCCCAGG ATGTGGGCCT TAAGCGAGGC 
GATCAGATTC ACGAAGTAAA TGGACAGTCG CTGGACCATG TGACTAGCAA GCGAGCCCTC 
GAGATACTCA CAGGCACCAC CCATTTGAGC ATCAGCGTTA AGAGCAACTT GCTTGGCTTC 
AAGGAGATTA TGCAGGCTCT TGAGCATGGC GGTGGAACCG CTGGATCCGG AAGCATTTCC 
GCAGGCAGCG GCAGTTTCAA GTCCGTGCGA AGTCCACGCC GTATTTGCGC CAATGATATT 
GCTAAGTTGC ACGGTCGCTC TGATAGCACC ACCGACGAAT TGTCCAGCGT CAGCGCCTCG 
AATCGGGCGC ACATGGTGCG CCTTAGTTCC GTCGATATGC TACTTGACCA GCCGGACTGC 
GCTCCACCGC AAACGCCGCC AGTGAGTGGG AGCGGTAACA TGGCCTCCAA TTTTATGCAG 
CAACTCTTGC AAAGCGTTAA CAATAGTTCG GCCAAGAAGT CCGGTGGGAA TTCAAACTCG 
GATCAGCAAG ATACGAAGGG CGGTTTTATG ACTCTGGCGC CAAAGCGTCG GTTGCAGAAA 
GCGCTGGCAA AGATGAATTT GCTCAATAAG CAAAATCACG GCAGCAGCCT AAACGACTCC 
TCGGATACGC TGCTCAACGA TCCCAAGTCA AAACTTTCTG CAGTTAGCTC GTGCAGCAGT 
TCCACTCAGT CGTCTATCAA TGGATGCACG GTCAGTGGGG GTGGCCGGCT CTACCAGTCG 
CAATCGAATC CGGATTTAAC CAGTCTCAAC TATGACGGAG GCAGCGATGC GGGTGGAAAC 
GGTGGTGGAA GATTGCAAGT GAACTACCTG AACGCCCACA TTCACCGGCC ATCAGCGGCC 
AGTACTTTGA CAACAAACTC GACGCAATCG CACCTCTTGC CCGATTACCC GGACCACGTG 
CTCAAGGTGT ACAAGGCAGA CCAGACGTGC AAGTATGTAC TCATCTACAA GGAGACGACA 
GCCCACGAGG TCGTAATGCT GACGCTGCAG GAGTTTGGAA TACACGACCC CAGCTCGAAT 
TTCTCGCTAT GCGAAGTGAG TGTTGGCGAC GGGGGTATGG TAAAGCAGCG CCGGTTGCCC 
GATCAGCTGC AGAACCTGGC CGAGCGAATA AGCTTTGCGG CGCGTTACTA CCTCAAGTTA 
AACGATAGCA CCGAACCGCT GGTTCCAGAC GAACTGGCCC TGGAACTGGT ACGCGAGTCG 
AACGTGCACT TCCTGCACCT GAATGCATAC GAGCTGGCCA TTCAGCTCAC TCTACAGGAC 
TTTGCGAACT TTCGCCAGAT TGAGTCCACC GAGTACGTGG ACGAATTGTT CGAGTTGCGC 
TCACGCTACG GAGTGCCAAT GCTGAGCAAG TTTGCCGAAC TGGTCAATCG TGAAATGTTT 
TGGGTTGTGA GTGAGATTTG CGCAGAGCAC AACATTGTGC GCCGCATGAA GATCGTCAAG 
CAGTTCATCA AAATTGCGCG CCACTGCAAG GAGTGCCGTA ACTTTAACTC CATGTTTGCA 
ATCGTCTCGG GGCTTGGACA CGGAGCTGTT TCGCGACTGC GTCAAACGTG GGAGAAGCTG 
CCCTCCAAAT ATCAGCGACT GTTCAACGAC CTGCAGGATC TGATGGATCC CTCGCGCAAC 
ATGAGCAAGT ATCGGCAACT AGTGTCCGCC GAACTACTGG CCCAGCACCC CATCATCCCG 
TTCTATCCGA TCGTCAAGAA GGATCTCACC TTTATTCACC TGGGCAATGA TACGAGAGTT 
GATGGCCTAA TCAACTTTGA GAAGCTGCGA ATGCTGGCCA AGGAGGTACG CCTGCTCACG 
CACATGTGCA GTTCGCCATA CGATCTGCTG TCGATCCTTG AGCTCAAGGG GCAGTCGCCA 
TCCAACGCTC TCTTCTCGCT CAATCAGATG TCTGCATCGC AGAGCAACGC TGCCGCTGGC 
ACAGTTATCG CCGCCAATGC CGGCCAGGCG ACTATCAAGC GGCGCAAGAA GTCGACGGCG 
GCGCCCAATC CCAAGAAGAT GTTTGAGGAG GCGCAGATGG TGCGGCGGGT GAAAGCATAT 
CTCAACAGCC TCAAAATACT CAGTGACGAG GACCTTTTGC ACAAATTCTC GCTGGAATGC 
GAGCCCGCGC ACGGGTCCAC GTACTCGGGC AGCATCTCGC ACGGAAATAC CTCACACCGA 
AGCGGTGGTG GAGGCAGCAT CAGTGGCGGT GCTGGTGGCA GCTCCGGTGG TGGTGGCGGG 
GGCAGTTCCA GCCTCAATGC CGGCGACCAA CTGAGCATCT ATTCGCATAC CAGCTCCAGC 
TCGGCGCCGA ACTCCTCACT ATCGCTTCGC AAGAGGCACC CAAGTTCACC CACCCTTTCG 
ACTACCAGCT CAACGAGTTC TACCAGCGAT CATCAAAGAA GACAGATGCA TAACAACGGC 
CCAAAGTTTG GCACAGCCTC GCCACAAGCA GTTAAAAAGA TGCTATCGCT GTCAGAGTCC 
TCCAAGATTC GGCCGCACCA GCCATTCGTG CCGCGTCACG GATCCACAAT GGCCGGAGTG 
ATTCCACCAC TGCATCACAT GCATGCGGCA CATGGTTTCA GCACACCGTC GCCTGGAGGA 
GTGGTCACCT CACCGGCAAC CAGTGCAGTG GCCAATGTTC AGTGTACGCC AAGTCCTAGT 
CCCTGCTCCC ACCGACGACT GGCTTCTGGA GGCAACATTA TTCCCTCTCG CGCAATACAC 
GAGCGGTCGC ACTCGGACAC ACCAGCTCCG CCGCCGCCTC TACCCTCGGT CGATCTCTCC 
CTAGAGAGCA GTAGTGTAAC TACATTTCGC GATTTACCAT TGCGCAAATC CGTGACTTCC 
GGTGGCCCGC CAAATTTTAT GGATAGCACC AAGTGTACCA TATGTCCCAT GCCACCCATG 
AATCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137798.1
CDS	448..4716
Translation	MDPYHHIRHHYPPTSIAGTGVVVGSSTTINRPELHQKCNRGSHSSDTSSAYSGSDTMASNYASSLEAEEIDLSGLVESVVDSDEEDLAESMDSLTVRDAVRDCLEKDPAERSEEDVEVLLEFTQGLKAFTNITLAVRRALCSVMVFAVVDKAGTVVMSDGEELDSWSVLINGAVEIEHANGSREELQMGDSFGILPTMDKLYHRGVMRTKCDDCQFVCITQTDYYRIQHQGEENTRRHEDENGFVVMVTELRSIGGAGTDSAGSGGSATGASASLNMKRGHVVIRGTPERLLQQLVEENSMTDPTYVEDFLLTHRIFIQNPQEVTSKLLHWFDLEQVDAHKTQELRDRVTRVVLLWVNNHFTDFEADYEMMEFLEVFEALLERKKLLSQLRLLHIACAAKARMRSCTLTRSSRDEPLNFRIVGGYELRGVAIATGNAAVGIYISHVEPGSKAQDVGLKRGDQIHEVNGQSLDHVTSKRALEILTGTTHLSISVKSNLLGFKEIMQALEHGGGTAGSGSISAGSGSFKSVRSPRRICANDIAKLHGRSDSTTDELSSVSASNRAHMVRLSSVDMLLDQPDCAPPQTPPVSGSGNMASNFMQQLLQSVNNSSAKKSGGNSNSDQQDTKGGFMTLAPKRRLQKALAKMNLLNKQNHGSSLNDSSDTLLNDPKSKLSAVSSCSSSTQSSINGCTVSGGGRLYQSQSNPDLTSLNYDGGSDAGGNGGGRLQVNYLNAHIHRPSAASTLTTNSTQSHLLPDYPDHVLKVYKADQTCKYVLIYKETTAHEVVMLTLQEFGIHDPSSNFSLCEVSVGDGGMVKQRRLPDQLQNLAERISFAARYYLKLNDSTEPLVPDELALELVRESNVHFLHLNAYELAIQLTLQDFANFRQIESTEYVDELFELRSRYGVPMLSKFAELVNREMFWVVSEICAEHNIVRRMKIVKQFIKIARHCKECRNFNSMFAIVSGLGHGAVSRLRQTWEKLPSKYQRLFNDLQDLMDPSRNMSKYRQLVSAELLAQHPIIPFYPIVKKDLTFIHLGNDTRVDGLINFEKLRMLAKEVRLLTHMCSSPYDLLSILELKGQSPSNALFSLNQMSASQSNAAAGTVIAANAGQATIKRRKKSTAAPNPKKMFEEAQMVRRVKAYLNSLKILSDEDLLHKFSLECEPAHGSTYSGSISHGNTSHRSGGGGSISGGAGGSSGGGGGGSSSLNAGDQLSIYSHTSSSSAPNSSLSLRKRHPSSPTLSTTSSTSSTSDHQRRQMHNNGPKFGTASPQAVKKMLSLSESSKIRPHQPFVPRHGSTMAGVIPPLHHMHAAHGFSTPSPGGVVTSPATSAVANVQCTPSPSPCSHRRLASGGNIIPSRAIHERSHSDTPAPPPPLPSVDLSLESSSVTTFRDLPLRKSVTSGGPPNFMDSTKCTICPMPPMNQ

Target ORF information:

RefSeq Version	NM_001144326.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PDZ domain-containing guanine nucleotide exchange factor (PDZ-GEF), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144326.2

ORF Insert Sequence:

ATGGATCCGT ATCACCATAT CAGACATCAT TATCCACCCA CAAGCATAGC AGGCACAGGA 
GTGGTGGTGG GCAGCTCGAC TACAATCAAC CGGCCGGAAC TGCACCAAAA ATGCAACCGG 
GGCTCGCACT CCAGTGACAC CAGTTCGGCG TACAGCGGCA GCGATACAAT GGCCTCGAAC 
TATGCCTCGT CGCTGGAGGC CGAGGAGATT GACCTCTCCG GCCTGGTGGA GTCCGTTGTG 
GACTCTGACG AGGAGGACCT GGCGGAGAGC ATGGATAGTC TTACCGTGCG GGATGCCGTG 
CGTGACTGTC TGGAAAAGGA TCCGGCGGAG CGCAGCGAGG AAGATGTGGA AGTACTGCTT 
GAGTTCACGC AGGGCCTTAA GGCATTCACA AACATTACGC TGGCAGTGCG AAGAGCACTA 
TGTTCCGTGA TGGTATTCGC CGTTGTGGAC AAGGCGGGAA CCGTGGTCAT GTCAGATGGT 
GAGGAACTGG ACTCATGGTC GGTGCTCATA AACGGAGCGG TGGAGATCGA GCATGCAAAT 
GGCAGTCGCG AAGAGCTGCA GATGGGAGAC TCCTTCGGCA TTCTACCCAC CATGGACAAG 
CTGTACCACC GGGGTGTGAT GCGTACGAAG TGCGATGACT GCCAATTTGT GTGCATTACG 
CAGACGGACT ACTACCGTAT TCAGCATCAA GGCGAGGAGA ATACCCGGCG ACATGAGGAC 
GAGAATGGTT TCGTTGTTAT GGTCACTGAA CTCCGGTCCA TTGGCGGCGC CGGCACTGAT 
TCTGCTGGCA GTGGCGGATC GGCGACAGGA GCTTCGGCTT CGCTAAATAT GAAGCGTGGT 
CACGTAGTTA TCCGCGGTAC TCCAGAGCGT CTGCTACAGC AGCTGGTCGA GGAAAACTCG 
ATGACCGATC CCACATACGT GGAGGACTTT TTGCTCACAC ACCGCATCTT TATTCAGAAC 
CCACAAGAGG TGACCAGCAA GTTGCTGCAT TGGTTTGATT TGGAGCAGGT GGATGCGCAC 
AAGACGCAGG AACTGCGAGA TCGCGTAACA CGCGTCGTGC TCCTCTGGGT GAACAACCAT 
TTCACCGATT TCGAGGCGGA CTATGAGATG ATGGAGTTTC TGGAAGTCTT TGAGGCACTG 
TTAGAGCGAA AAAAGTTGCT AAGTCAGCTG CGCCTGTTGC ACATAGCCTG TGCCGCCAAA 
GCACGGATGC GCAGCTGTAC CCTCACTCGA TCGTCACGTG ACGAGCCGCT CAACTTCCGC 
ATCGTGGGCG GCTACGAGCT TCGAGGCGTC GCCATAGCCA CTGGAAACGC AGCAGTGGGC 
ATCTACATTT CGCACGTCGA GCCGGGATCA AAAGCCCAGG ATGTGGGCCT TAAGCGAGGC 
GATCAGATTC ACGAAGTAAA TGGACAGTCG CTGGACCATG TGACTAGCAA GCGAGCCCTC 
GAGATACTCA CAGGCACCAC CCATTTGAGC ATCAGCGTTA AGAGCAACTT GCTTGGCTTC 
AAGGAGATTA TGCAGGCTCT TGAGCATGGC GGTGGAACCG CTGGATCCGG AAGCATTTCC 
GCAGGCAGCG GCAGTTTCAA GTCCGTGCGA AGTCCACGCC GTATTTGCGC CAATGATATT 
GCTAAGTTGC ACGGTCGCTC TGATAGCACC ACCGACGAAT TGTCCAGCGT CAGCGCCTCG 
AATCGGGCGC ACATGGTGCG CCTTAGTTCC GTCGATATGC TACTTGACCA GCCGGACTGC 
GCTCCACCGC AAACGCCGCC AGTGAGTGGG AGCGGTAACA TGGCCTCCAA TTTTATGCAG 
CAACTCTTGC AAAGCGTTAA CAATAGTTCG GCCAAGAAGT CCGGTGGGAA TTCAAACTCG 
GATCAGCAAG ATACGAAGGG CGGTTTTATG ACTCTGGCGC CAAAGCGTCG GTTGCAGAAA 
GCGCTGGCAA AGATGAATTT GCTCAATAAG CAAAATCACG GCAGCAGCCT AAACGACTCC 
TCGGATACGC TGCTCAACGA TCCCAAGTCA AAACTTTCTG CAGTTAGCTC GTGCAGCAGT 
TCCACTCAGT CGTCTATCAA TGGATGCACG GTCAGTGGGG GTGGCCGGCT CTACCAGTCG 
CAATCGAATC CGGATTTAAC CAGTCTCAAC TATGACGGAG GCAGCGATGC GGGTGGAAAC 
GGTGGTGGAA GATTGCAAGT GAACTACCTG AACGCCCACA TTCACCGGCC ATCAGCGGCC 
AGTACTTTGA CAACAAACTC GACGCAATCG CACCTCTTGC CCGATTACCC GGACCACGTG 
CTCAAGGTGT ACAAGGCAGA CCAGACGTGC AAGTATGTAC TCATCTACAA GGAGACGACA 
GCCCACGAGG TCGTAATGCT GACGCTGCAG GAGTTTGGAA TACACGACCC CAGCTCGAAT 
TTCTCGCTAT GCGAAGTGAG TGTTGGCGAC GGGGGTATGG TAAAGCAGCG CCGGTTGCCC 
GATCAGCTGC AGAACCTGGC CGAGCGAATA AGCTTTGCGG CGCGTTACTA CCTCAAGTTA 
AACGATAGCA CCGAACCGCT GGTTCCAGAC GAACTGGCCC TGGAACTGGT ACGCGAGTCG 
AACGTGCACT TCCTGCACCT GAATGCATAC GAGCTGGCCA TTCAGCTCAC TCTACAGGAC 
TTTGCGAACT TTCGCCAGAT TGAGTCCACC GAGTACGTGG ACGAATTGTT CGAGTTGCGC 
TCACGCTACG GAGTGCCAAT GCTGAGCAAG TTTGCCGAAC TGGTCAATCG TGAAATGTTT 
TGGGTTGTGA GTGAGATTTG CGCAGAGCAC AACATTGTGC GCCGCATGAA GATCGTCAAG 
CAGTTCATCA AAATTGCGCG CCACTGCAAG GAGTGCCGTA ACTTTAACTC CATGTTTGCA 
ATCGTCTCGG GGCTTGGACA CGGAGCTGTT TCGCGACTGC GTCAAACGTG GGAGAAGCTG 
CCCTCCAAAT ATCAGCGACT GTTCAACGAC CTGCAGGATC TGATGGATCC CTCGCGCAAC 
ATGAGCAAGT ATCGGCAACT AGTGTCCGCC GAACTACTGG CCCAGCACCC CATCATCCCG 
TTCTATCCGA TCGTCAAGAA GGATCTCACC TTTATTCACC TGGGCAATGA TACGAGAGTT 
GATGGCCTAA TCAACTTTGA GAAGCTGCGA ATGCTGGCCA AGGAGGTACG CCTGCTCACG 
CACATGTGCA GTTCGCCATA CGATCTGCTG TCGATCCTTG AGCTCAAGGG GCAGTCGCCA 
TCCAACGCTC TCTTCTCGCT CAATCAGATG TCTGCATCGC AGAGCAACGC TGCCGCTGGC 
ACAGTTATCG CCGCCAATGC CGGCCAGGCG ACTATCAAGC GGCGCAAGAA GTCGACGGCG 
GCGCCCAATC CCAAGAAGAT GTTTGAGGAG GCGCAGATGG TGCGGCGGGT GAAAGCATAT 
CTCAACAGCC TCAAAATACT CAGTGACGAG GACCTTTTGC ACAAATTCTC GCTGGAATGC 
GAGCCCGCGC ACGGGTCCAC GTACTCGGGC AGCATCTCGC ACGGAAATAC CTCACACCGA 
AGCGGTGGTG GAGGCAGCAT CAGTGGCGGT GCTGGTGGCA GCTCCGGTGG TGGTGGCGGG 
GGCAGTTCCA GCCTCAATGC CGGCGACCAA CTGAGCATCT ATTCGCATAC CAGCTCCAGC 
TCGGCGCCGA ACTCCTCACT ATCGCTTCGC AAGAGGCACC CAAGTTCACC CACCCTTTCG 
ACTACCAGCT CAACGAGTTC TACCAGCGAT CATCAAAGAA GACAGATGCA TAACAACGGC 
CCAAAGTTTG GCACAGCCTC GCCACAAGCA GTTAAAAAGA TGCTATCGCT GTCAGAGTCC 
TCCAAGATTC GGCCGCACCA GCCATTCGTG CCGCGTCACG GATCCACAAT GGCCGGAGTG 
ATTCCACCAC TGCATCACAT GCATGCGGCA CATGGTTTCA GCACACCGTC GCCTGGAGGA 
GTGGTCACCT CACCGGCAAC CAGTGCAGTG GCCAATGTTC AGTGTACGCC AAGTCCTAGT 
CCCTGCTCCC ACCGACGACT GGCTTCTGGA GGCAACATTA TTCCCTCTCG CGCAATACAC 
GAGCGGTCGC ACTCGGACAC ACCAGCTCCG CCGCCGCCTC TACCCTCGGT CGATCTCTCC 
CTAGAGAGCA GTAGTGTAAC TACATTTCGC GATTTACCAT TGCGCAAATC CGTGACTTCC 
GGTGGCCCGC CAAATTTTAT GGATAGCACC AAGTGTACCA TATGTCCCAT GCCACCCATG 
AATCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDZ-GEF ( NM_135168.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609012.2 Drosophila melanogaster PDZ domain-containing guanine nucleotide exchange factor (PDZ-GEF), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13892
Clone ID Related Accession (Same CDS sequence)	NM_135168.5
Accession Version	NM_135168.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PDZ domain-containing guanine nucleotide exchange factor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135168.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCCGT ATCACCATAT CAGACATCAT TATCCACCCA CAAGCATAGC AGGCACAGGA 
GTGGTGGTGG GCAGCTCGAC TACAATCAAC CGGCCGGAAC TGCACCAAAA ATGCAACCGG 
GGCTCGCACT CCAGTGACAC CAGTTCGGCG TACAGCGGCA GCGATACAAT GGCCTCGAAC 
TATGCCTCGT CGCTGGAGGC CGAGGAGATT GACCTCTCCG GCCTGGTGGA GTCCGTTGTG 
GACTCTGACG AGGAGGACCT GGCGGAGAGC ATGGATAGTC TTACCGTGCG GGATGCCGTG 
CGTGACTGTC TGGAAAAGGA TCCGGCGGAG CGCAGCGAGG AAGATGTGGA AGTACTGCTT 
GAGTTCACGC AGGGCCTTAA GGCATTCACA AACATTACGC TGGCAGTGCG AAGAGCACTA 
TGTTCCGTGA TGGTATTCGC CGTTGTGGAC AAGGCGGGAA CCGTGGTCAT GTCAGATGGT 
GAGGAACTGG ACTCATGGTC GGTGCTCATA AACGGAGCGG TGGAGATCGA GCATGCAAAT 
GGCAGTCGCG AAGAGCTGCA GATGGGAGAC TCCTTCGGCA TTCTACCCAC CATGGACAAG 
CTGTACCACC GGGGTGTGAT GCGTACGAAG TGCGATGACT GCCAATTTGT GTGCATTACG 
CAGACGGACT ACTACCGTAT TCAGCATCAA GGCGAGGAGA ATACCCGGCG ACATGAGGAC 
GAGAATGGTT TCGTTGTTAT GGTCACTGAA CTCCGGTCCA TTGGCGGCGC CGGCACTGAT 
TCTGCTGGCA GTGGCGGATC GGCGACAGGA GCTTCGGCTT CGCTAAATAT GAAGCGTGGT 
CACGTAGTTA TCCGCGGTAC TCCAGAGCGT CTGCTACAGC AGCTGGTCGA GGAAAACTCG 
ATGACCGATC CCACATACGT GGAGGACTTT TTGCTCACAC ACCGCATCTT TATTCAGAAC 
CCACAAGAGG TGACCAGCAA GTTGCTGCAT TGGTTTGATT TGGAGCAGGT GGATGCGCAC 
AAGACGCAGG AACTGCGAGA TCGCGTAACA CGCGTCGTGC TCCTCTGGGT GAACAACCAT 
TTCACCGATT TCGAGGCGGA CTATGAGATG ATGGAGTTTC TGGAAGTCTT TGAGGCACTG 
TTAGAGCGAA AAAAGTTGCT AAGTCAGCTG CGCCTGTTGC ACATAGCCTG TGCCGCCAAA 
GCACGGATGC GCAGCTGTAC CCTCACTCGA TCGTCACGTG ACGAGCCGCT CAACTTCCGC 
ATCGTGGGCG GCTACGAGCT TCGAGGCGTC GCCATAGCCA CTGGAAACGC AGCAGTGGGC 
ATCTACATTT CGCACGTCGA GCCGGGATCA AAAGCCCAGG ATGTGGGCCT TAAGCGAGGC 
GATCAGATTC ACGAAGTAAA TGGACAGTCG CTGGACCATG TGACTAGCAA GCGAGCCCTC 
GAGATACTCA CAGGCACCAC CCATTTGAGC ATCAGCGTTA AGAGCAACTT GCTTGGCTTC 
AAGGAGATTA TGCAGGCTCT TGAGCATGGC GGTGGAACCG CTGGATCCGG AAGCATTTCC 
GCAGGCAGCG GCAGTTTCAA GTCCGTGCGA AGTCCACGCC GTATTTGCGC CAATGATATT 
GCTAAGTTGC ACGGTCGCTC TGATAGCACC ACCGACGAAT TGTCCAGCGT CAGCGCCTCG 
AATCGGGCGC ACATGGTGCG CCTTAGTTCC GTCGATATGC TACTTGACCA GCCGGACTGC 
GCTCCACCGC AAACGCCGCC AGTGAGTGGG AGCGGTAACA TGGCCTCCAA TTTTATGCAG 
CAACTCTTGC AAAGCGTTAA CAATAGTTCG GCCAAGAAGT CCGGTGGGAA TTCAAACTCG 
GATCAGCAAG ATACGAAGGG CGGTTTTATG ACTCTGGCGC CAAAGCGTCG GTTGCAGAAA 
GCGCTGGCAA AGATGAATTT GCTCAATAAG CAAAATCACG GCAGCAGCCT AAACGACTCC 
TCGGATACGC TGCTCAACGA TCCCAAGTCA AAACTTTCTG CAGTTAGCTC GTGCAGCAGT 
TCCACTCAGT CGTCTATCAA TGGATGCACG GTCAGTGGGG GTGGCCGGCT CTACCAGTCG 
CAATCGAATC CGGATTTAAC CAGTCTCAAC TATGACGGAG GCAGCGATGC GGGTGGAAAC 
GGTGGTGGAA GATTGCAAGT GAACTACCTG AACGCCCACA TTCACCGGCC ATCAGCGGCC 
AGTACTTTGA CAACAAACTC GACGCAATCG CACCTCTTGC CCGATTACCC GGACCACGTG 
CTCAAGGTGT ACAAGGCAGA CCAGACGTGC AAGTATGTAC TCATCTACAA GGAGACGACA 
GCCCACGAGG TCGTAATGCT GACGCTGCAG GAGTTTGGAA TACACGACCC CAGCTCGAAT 
TTCTCGCTAT GCGAAGTGAG TGTTGGCGAC GGGGGTATGG TAAAGCAGCG CCGGTTGCCC 
GATCAGCTGC AGAACCTGGC CGAGCGAATA AGCTTTGCGG CGCGTTACTA CCTCAAGTTA 
AACGATAGCA CCGAACCGCT GGTTCCAGAC GAACTGGCCC TGGAACTGGT ACGCGAGTCG 
AACGTGCACT TCCTGCACCT GAATGCATAC GAGCTGGCCA TTCAGCTCAC TCTACAGGAC 
TTTGCGAACT TTCGCCAGAT TGAGTCCACC GAGTACGTGG ACGAATTGTT CGAGTTGCGC 
TCACGCTACG GAGTGCCAAT GCTGAGCAAG TTTGCCGAAC TGGTCAATCG TGAAATGTTT 
TGGGTTGTGA GTGAGATTTG CGCAGAGCAC AACATTGTGC GCCGCATGAA GATCGTCAAG 
CAGTTCATCA AAATTGCGCG CCACTGCAAG GAGTGCCGTA ACTTTAACTC CATGTTTGCA 
ATCGTCTCGG GGCTTGGACA CGGAGCTGTT TCGCGACTGC GTCAAACGTG GGAGAAGCTG 
CCCTCCAAAT ATCAGCGACT GTTCAACGAC CTGCAGGATC TGATGGATCC CTCGCGCAAC 
ATGAGCAAGT ATCGGCAACT AGTGTCCGCC GAACTACTGG CCCAGCACCC CATCATCCCG 
TTCTATCCGA TCGTCAAGAA GGATCTCACC TTTATTCACC TGGGCAATGA TACGAGAGTT 
GATGGCCTAA TCAACTTTGA GAAGCTGCGA ATGCTGGCCA AGGAGGTACG CCTGCTCACG 
CACATGTGCA GTTCGCCATA CGATCTGCTG TCGATCCTTG AGCTCAAGGG GCAGTCGCCA 
TCCAACGCTC TCTTCTCGCT CAATCAGATG TCTGCATCGC AGAGCAACGC TGCCGCTGGC 
ACAGTTATCG CCGCCAATGC CGGCCAGGCG ACTATCAAGC GGCGCAAGAA GTCGACGGCG 
GCGCCCAATC CCAAGAAGAT GTTTGAGGAG GCGCAGATGG TGCGGCGGGT GAAAGCATAT 
CTCAACAGCC TCAAAATACT CAGTGACGAG GACCTTTTGC ACAAATTCTC GCTGGAATGC 
GAGCCCGCGC ACGGGTCCAC GTACTCGGGC AGCATCTCGC ACGGAAATAC CTCACACCGA 
AGCGGTGGTG GAGGCAGCAT CAGTGGCGGT GCTGGTGGCA GCTCCGGTGG TGGTGGCGGG 
GGCAGTTCCA GCCTCAATGC CGGCGACCAA CTGAGCATCT ATTCGCATAC CAGCTCCAGC 
TCGGCGCCGA ACTCCTCACT ATCGCTTCGC AAGAGGCACC CAAGTTCACC CACCCTTTCG 
ACTACCAGCT CAACGAGTTC TACCAGCGAT CATCAAAGAA GACAGATGCA TAACAACGGC 
CCAAAGTTTG GCACAGCCTC GCCACAAGCA GTTAAAAAGA TGCTATCGCT GTCAGAGTCC 
TCCAAGATTC GGCCGCACCA GCCATTCGTG CCGCGTCACG GATCCACAAT GGCCGGAGTG 
ATTCCACCAC TGCATCACAT GCATGCGGCA CATGGTTTCA GCACACCGTC GCCTGGAGGA 
GTGGTCACCT CACCGGCAAC CAGTGCAGTG GCCAATGTTC AGTGTACGCC AAGTCCTAGT 
CCCTGCTCCC ACCGACGACT GGCTTCTGGA GGCAACATTA TTCCCTCTCG CGCAATACAC 
GAGCGGTCGC ACTCGGACAC ACCAGCTCCG CCGCCGCCTC TACCCTCGGT CGATCTCTCC 
CTAGAGAGCA GTAGTGTAAC TACATTTCGC GATTTACCAT TGCGCAAATC CGTGACTTCC 
GGTTCCATAT CGTCCTGCGA CAGCGGCTAC GTGCATCAGC AGCAGCATTA CCACTTGCAG 
TACCAGCAGC AGCAACAACA GAACAGCTCG CAACACGAGC CATCCCCGCC GGTCTATACC 
GCAGCCGACT GCCGGCTGCT TCAGCAGATA TCGAACAACG CGGTGACCCG CAACTTGAAC 
AGTCCCTGTC AAAGCACAAA CACACCACCC AGCACACCTA CACCTCCACC GAACCAGCCC 
ACAGCAACCA TTCAGCTTTC TGCACCGCCG ACGGCAGCAG CATACATGCA CGCACGCAGC 
CAGCATCAGC AGCTCCAGCA GCAGCAGCAA TCTTTGGCCA TGCCACCACC GCCGCCACCG 
CCGTACAATG TGCCCCCCCT GGGCAGTATC TACAGCCACC ACCAGGGCAC TGCTGGCAGC 
AGGCACCTGA ACCACATGCA TGGTAAGACG ACCGGACCGC AAGAGAGATG GTTTCCAGAT 
TGCCGTCCAA CGACAAAACA ACAAATGCAA AGCGGGAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609012.2
CDS	448..5169
Translation	MDPYHHIRHHYPPTSIAGTGVVVGSSTTINRPELHQKCNRGSHSSDTSSAYSGSDTMASNYASSLEAEEIDLSGLVESVVDSDEEDLAESMDSLTVRDAVRDCLEKDPAERSEEDVEVLLEFTQGLKAFTNITLAVRRALCSVMVFAVVDKAGTVVMSDGEELDSWSVLINGAVEIEHANGSREELQMGDSFGILPTMDKLYHRGVMRTKCDDCQFVCITQTDYYRIQHQGEENTRRHEDENGFVVMVTELRSIGGAGTDSAGSGGSATGASASLNMKRGHVVIRGTPERLLQQLVEENSMTDPTYVEDFLLTHRIFIQNPQEVTSKLLHWFDLEQVDAHKTQELRDRVTRVVLLWVNNHFTDFEADYEMMEFLEVFEALLERKKLLSQLRLLHIACAAKARMRSCTLTRSSRDEPLNFRIVGGYELRGVAIATGNAAVGIYISHVEPGSKAQDVGLKRGDQIHEVNGQSLDHVTSKRALEILTGTTHLSISVKSNLLGFKEIMQALEHGGGTAGSGSISAGSGSFKSVRSPRRICANDIAKLHGRSDSTTDELSSVSASNRAHMVRLSSVDMLLDQPDCAPPQTPPVSGSGNMASNFMQQLLQSVNNSSAKKSGGNSNSDQQDTKGGFMTLAPKRRLQKALAKMNLLNKQNHGSSLNDSSDTLLNDPKSKLSAVSSCSSSTQSSINGCTVSGGGRLYQSQSNPDLTSLNYDGGSDAGGNGGGRLQVNYLNAHIHRPSAASTLTTNSTQSHLLPDYPDHVLKVYKADQTCKYVLIYKETTAHEVVMLTLQEFGIHDPSSNFSLCEVSVGDGGMVKQRRLPDQLQNLAERISFAARYYLKLNDSTEPLVPDELALELVRESNVHFLHLNAYELAIQLTLQDFANFRQIESTEYVDELFELRSRYGVPMLSKFAELVNREMFWVVSEICAEHNIVRRMKIVKQFIKIARHCKECRNFNSMFAIVSGLGHGAVSRLRQTWEKLPSKYQRLFNDLQDLMDPSRNMSKYRQLVSAELLAQHPIIPFYPIVKKDLTFIHLGNDTRVDGLINFEKLRMLAKEVRLLTHMCSSPYDLLSILELKGQSPSNALFSLNQMSASQSNAAAGTVIAANAGQATIKRRKKSTAAPNPKKMFEEAQMVRRVKAYLNSLKILSDEDLLHKFSLECEPAHGSTYSGSISHGNTSHRSGGGGSISGGAGGSSGGGGGGSSSLNAGDQLSIYSHTSSSSAPNSSLSLRKRHPSSPTLSTTSSTSSTSDHQRRQMHNNGPKFGTASPQAVKKMLSLSESSKIRPHQPFVPRHGSTMAGVIPPLHHMHAAHGFSTPSPGGVVTSPATSAVANVQCTPSPSPCSHRRLASGGNIIPSRAIHERSHSDTPAPPPPLPSVDLSLESSSVTTFRDLPLRKSVTSGSISSCDSGYVHQQQHYHLQYQQQQQQNSSQHEPSPPVYTAADCRLLQQISNNAVTRNLNSPCQSTNTPPSTPTPPPNQPTATIQLSAPPTAAAYMHARSQHQQLQQQQQSLAMPPPPPPPYNVPPLGSIYSHHQGTAGSRHLNHMHGKTTGPQERWFPDCRPTTKQQMQSGS

Target ORF information:

RefSeq Version	NM_135168.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PDZ domain-containing guanine nucleotide exchange factor (PDZ-GEF), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135168.5

ORF Insert Sequence:

ATGGATCCGT ATCACCATAT CAGACATCAT TATCCACCCA CAAGCATAGC AGGCACAGGA 
GTGGTGGTGG GCAGCTCGAC TACAATCAAC CGGCCGGAAC TGCACCAAAA ATGCAACCGG 
GGCTCGCACT CCAGTGACAC CAGTTCGGCG TACAGCGGCA GCGATACAAT GGCCTCGAAC 
TATGCCTCGT CGCTGGAGGC CGAGGAGATT GACCTCTCCG GCCTGGTGGA GTCCGTTGTG 
GACTCTGACG AGGAGGACCT GGCGGAGAGC ATGGATAGTC TTACCGTGCG GGATGCCGTG 
CGTGACTGTC TGGAAAAGGA TCCGGCGGAG CGCAGCGAGG AAGATGTGGA AGTACTGCTT 
GAGTTCACGC AGGGCCTTAA GGCATTCACA AACATTACGC TGGCAGTGCG AAGAGCACTA 
TGTTCCGTGA TGGTATTCGC CGTTGTGGAC AAGGCGGGAA CCGTGGTCAT GTCAGATGGT 
GAGGAACTGG ACTCATGGTC GGTGCTCATA AACGGAGCGG TGGAGATCGA GCATGCAAAT 
GGCAGTCGCG AAGAGCTGCA GATGGGAGAC TCCTTCGGCA TTCTACCCAC CATGGACAAG 
CTGTACCACC GGGGTGTGAT GCGTACGAAG TGCGATGACT GCCAATTTGT GTGCATTACG 
CAGACGGACT ACTACCGTAT TCAGCATCAA GGCGAGGAGA ATACCCGGCG ACATGAGGAC 
GAGAATGGTT TCGTTGTTAT GGTCACTGAA CTCCGGTCCA TTGGCGGCGC CGGCACTGAT 
TCTGCTGGCA GTGGCGGATC GGCGACAGGA GCTTCGGCTT CGCTAAATAT GAAGCGTGGT 
CACGTAGTTA TCCGCGGTAC TCCAGAGCGT CTGCTACAGC AGCTGGTCGA GGAAAACTCG 
ATGACCGATC CCACATACGT GGAGGACTTT TTGCTCACAC ACCGCATCTT TATTCAGAAC 
CCACAAGAGG TGACCAGCAA GTTGCTGCAT TGGTTTGATT TGGAGCAGGT GGATGCGCAC 
AAGACGCAGG AACTGCGAGA TCGCGTAACA CGCGTCGTGC TCCTCTGGGT GAACAACCAT 
TTCACCGATT TCGAGGCGGA CTATGAGATG ATGGAGTTTC TGGAAGTCTT TGAGGCACTG 
TTAGAGCGAA AAAAGTTGCT AAGTCAGCTG CGCCTGTTGC ACATAGCCTG TGCCGCCAAA 
GCACGGATGC GCAGCTGTAC CCTCACTCGA TCGTCACGTG ACGAGCCGCT CAACTTCCGC 
ATCGTGGGCG GCTACGAGCT TCGAGGCGTC GCCATAGCCA CTGGAAACGC AGCAGTGGGC 
ATCTACATTT CGCACGTCGA GCCGGGATCA AAAGCCCAGG ATGTGGGCCT TAAGCGAGGC 
GATCAGATTC ACGAAGTAAA TGGACAGTCG CTGGACCATG TGACTAGCAA GCGAGCCCTC 
GAGATACTCA CAGGCACCAC CCATTTGAGC ATCAGCGTTA AGAGCAACTT GCTTGGCTTC 
AAGGAGATTA TGCAGGCTCT TGAGCATGGC GGTGGAACCG CTGGATCCGG AAGCATTTCC 
GCAGGCAGCG GCAGTTTCAA GTCCGTGCGA AGTCCACGCC GTATTTGCGC CAATGATATT 
GCTAAGTTGC ACGGTCGCTC TGATAGCACC ACCGACGAAT TGTCCAGCGT CAGCGCCTCG 
AATCGGGCGC ACATGGTGCG CCTTAGTTCC GTCGATATGC TACTTGACCA GCCGGACTGC 
GCTCCACCGC AAACGCCGCC AGTGAGTGGG AGCGGTAACA TGGCCTCCAA TTTTATGCAG 
CAACTCTTGC AAAGCGTTAA CAATAGTTCG GCCAAGAAGT CCGGTGGGAA TTCAAACTCG 
GATCAGCAAG ATACGAAGGG CGGTTTTATG ACTCTGGCGC CAAAGCGTCG GTTGCAGAAA 
GCGCTGGCAA AGATGAATTT GCTCAATAAG CAAAATCACG GCAGCAGCCT AAACGACTCC 
TCGGATACGC TGCTCAACGA TCCCAAGTCA AAACTTTCTG CAGTTAGCTC GTGCAGCAGT 
TCCACTCAGT CGTCTATCAA TGGATGCACG GTCAGTGGGG GTGGCCGGCT CTACCAGTCG 
CAATCGAATC CGGATTTAAC CAGTCTCAAC TATGACGGAG GCAGCGATGC GGGTGGAAAC 
GGTGGTGGAA GATTGCAAGT GAACTACCTG AACGCCCACA TTCACCGGCC ATCAGCGGCC 
AGTACTTTGA CAACAAACTC GACGCAATCG CACCTCTTGC CCGATTACCC GGACCACGTG 
CTCAAGGTGT ACAAGGCAGA CCAGACGTGC AAGTATGTAC TCATCTACAA GGAGACGACA 
GCCCACGAGG TCGTAATGCT GACGCTGCAG GAGTTTGGAA TACACGACCC CAGCTCGAAT 
TTCTCGCTAT GCGAAGTGAG TGTTGGCGAC GGGGGTATGG TAAAGCAGCG CCGGTTGCCC 
GATCAGCTGC AGAACCTGGC CGAGCGAATA AGCTTTGCGG CGCGTTACTA CCTCAAGTTA 
AACGATAGCA CCGAACCGCT GGTTCCAGAC GAACTGGCCC TGGAACTGGT ACGCGAGTCG 
AACGTGCACT TCCTGCACCT GAATGCATAC GAGCTGGCCA TTCAGCTCAC TCTACAGGAC 
TTTGCGAACT TTCGCCAGAT TGAGTCCACC GAGTACGTGG ACGAATTGTT CGAGTTGCGC 
TCACGCTACG GAGTGCCAAT GCTGAGCAAG TTTGCCGAAC TGGTCAATCG TGAAATGTTT 
TGGGTTGTGA GTGAGATTTG CGCAGAGCAC AACATTGTGC GCCGCATGAA GATCGTCAAG 
CAGTTCATCA AAATTGCGCG CCACTGCAAG GAGTGCCGTA ACTTTAACTC CATGTTTGCA 
ATCGTCTCGG GGCTTGGACA CGGAGCTGTT TCGCGACTGC GTCAAACGTG GGAGAAGCTG 
CCCTCCAAAT ATCAGCGACT GTTCAACGAC CTGCAGGATC TGATGGATCC CTCGCGCAAC 
ATGAGCAAGT ATCGGCAACT AGTGTCCGCC GAACTACTGG CCCAGCACCC CATCATCCCG 
TTCTATCCGA TCGTCAAGAA GGATCTCACC TTTATTCACC TGGGCAATGA TACGAGAGTT 
GATGGCCTAA TCAACTTTGA GAAGCTGCGA ATGCTGGCCA AGGAGGTACG CCTGCTCACG 
CACATGTGCA GTTCGCCATA CGATCTGCTG TCGATCCTTG AGCTCAAGGG GCAGTCGCCA 
TCCAACGCTC TCTTCTCGCT CAATCAGATG TCTGCATCGC AGAGCAACGC TGCCGCTGGC 
ACAGTTATCG CCGCCAATGC CGGCCAGGCG ACTATCAAGC GGCGCAAGAA GTCGACGGCG 
GCGCCCAATC CCAAGAAGAT GTTTGAGGAG GCGCAGATGG TGCGGCGGGT GAAAGCATAT 
CTCAACAGCC TCAAAATACT CAGTGACGAG GACCTTTTGC ACAAATTCTC GCTGGAATGC 
GAGCCCGCGC ACGGGTCCAC GTACTCGGGC AGCATCTCGC ACGGAAATAC CTCACACCGA 
AGCGGTGGTG GAGGCAGCAT CAGTGGCGGT GCTGGTGGCA GCTCCGGTGG TGGTGGCGGG 
GGCAGTTCCA GCCTCAATGC CGGCGACCAA CTGAGCATCT ATTCGCATAC CAGCTCCAGC 
TCGGCGCCGA ACTCCTCACT ATCGCTTCGC AAGAGGCACC CAAGTTCACC CACCCTTTCG 
ACTACCAGCT CAACGAGTTC TACCAGCGAT CATCAAAGAA GACAGATGCA TAACAACGGC 
CCAAAGTTTG GCACAGCCTC GCCACAAGCA GTTAAAAAGA TGCTATCGCT GTCAGAGTCC 
TCCAAGATTC GGCCGCACCA GCCATTCGTG CCGCGTCACG GATCCACAAT GGCCGGAGTG 
ATTCCACCAC TGCATCACAT GCATGCGGCA CATGGTTTCA GCACACCGTC GCCTGGAGGA 
GTGGTCACCT CACCGGCAAC CAGTGCAGTG GCCAATGTTC AGTGTACGCC AAGTCCTAGT 
CCCTGCTCCC ACCGACGACT GGCTTCTGGA GGCAACATTA TTCCCTCTCG CGCAATACAC 
GAGCGGTCGC ACTCGGACAC ACCAGCTCCG CCGCCGCCTC TACCCTCGGT CGATCTCTCC 
CTAGAGAGCA GTAGTGTAAC TACATTTCGC GATTTACCAT TGCGCAAATC CGTGACTTCC 
GGTTCCATAT CGTCCTGCGA CAGCGGCTAC GTGCATCAGC AGCAGCATTA CCACTTGCAG 
TACCAGCAGC AGCAACAACA GAACAGCTCG CAACACGAGC CATCCCCGCC GGTCTATACC 
GCAGCCGACT GCCGGCTGCT TCAGCAGATA TCGAACAACG CGGTGACCCG CAACTTGAAC 
AGTCCCTGTC AAAGCACAAA CACACCACCC AGCACACCTA CACCTCCACC GAACCAGCCC 
ACAGCAACCA TTCAGCTTTC TGCACCGCCG ACGGCAGCAG CATACATGCA CGCACGCAGC 
CAGCATCAGC AGCTCCAGCA GCAGCAGCAA TCTTTGGCCA TGCCACCACC GCCGCCACCG 
CCGTACAATG TGCCCCCCCT GGGCAGTATC TACAGCCACC ACCAGGGCAC TGCTGGCAGC 
AGGCACCTGA ACCACATGCA TGGTAAGACG ACCGGACCGC AAGAGAGATG GTTTCCAGAT 
TGCCGTCCAA CGACAAAACA ACAAATGCAA AGCGGGAGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDZ-GEF ( NM_001273197.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260126.1 Drosophila melanogaster PDZ domain-containing guanine nucleotide exchange factor (PDZ-GEF), transcript variant D, mRNA.

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CloneID	OFa15801
Clone ID Related Accession (Same CDS sequence)	NM_001273197.1 , NM_001144325.2
Accession Version	NM_001273197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PDZ domain-containing guanine nucleotide exchange factor, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCCGT ATCACCATAT CAGACATCAT TATCCACCCA CAAGCATAGC AGGCACAGGA 
GTGGTGGTGG GCAGCTCGAC TACAATCAAC CGGCCGGAAC TGCACCAAAA ATGCAACCGG 
GGCTCGCACT CCAGTGACAC CAGTTCGGCG TACAGCGGCA GCGATACAAT GGCCTCGAAC 
TATGCCTCGT CGCTGGAGGC CGAGGAGATT GACCTCTCCG GCCTGGTGGA GTCCGTTGTG 
GACTCTGACG AGGAGGACCT GGCGGAGAGC ATGGATAGTC TTACCGTGCG GGATGCCGTG 
CGTGACTGTC TGGAAAAGGA TCCGGCGGAG CGCAGCGAGG AAGATGTGGA AGTACTGCTT 
GAGTTCACGC AGGGCCTTAA GGCATTCACA AACATTACGC TGGCAGTGCG AAGAGCACTA 
TGTTCCGTGA TGGTATTCGC CGTTGTGGAC AAGGCGGGAA CCGTGGTCAT GTCAGATGGT 
GAGGAACTGG ACTCATGGTC GGTGCTCATA AACGGAGCGG TGGAGATCGA GCATGCAAAT 
GGCAGTCGCG AAGAGCTGCA GATGGGAGAC TCCTTCGGCA TTCTACCCAC CATGGACAAG 
CTGTACCACC GGGGTGTGAT GCGTACGAAG TGCGATGACT GCCAATTTGT GTGCATTACG 
CAGACGGACT ACTACCGTAT TCAGCATCAA GGCGAGGAGA ATACCCGGCG ACATGAGGAC 
GAGAATGGTT TCGTTGTTAT GGTCACTGAA CTCCGGTCCA TTGGCGGCGC CGGCACTGAT 
TCTGCTGGCA GTGGCGGATC GGCGACAGGA GCTTCGGCTT CGCTAAATAT GAAGCGTGGT 
CACGTAGTTA TCCGCGGTAC TCCAGAGCGT CTGCTACAGC AGCTGGTCGA GGAAAACTCG 
ATGACCGATC CCACATACGT GGAGGACTTT TTGCTCACAC ACCGCATCTT TATTCAGAAC 
CCACAAGAGG TGACCAGCAA GTTGCTGCAT TGGTTTGATT TGGAGCAGGT GGATGCGCAC 
AAGACGCAGG AACTGCGAGA TCGCGTAACA CGCGTCGTGC TCCTCTGGGT GAACAACCAT 
TTCACCGATT TCGAGGCGGA CTATGAGATG ATGGAGTTTC TGGAAGTCTT TGAGGCACTG 
TTAGAGCGAA AAAAGTTGCT AAGTCAGCTG CGCCTGTTGC ACATAGCCTG TGCCGCCAAA 
GCACGGATGC GCAGCTGTAC CCTCACTCGA TCGTCACGTG ACGAGCCGCT CAACTTCCGC 
ATCGTGGGCG GCTACGAGCT TCGAGGCGTC GCCATAGCCA CTGGAAACGC AGCAGTGGGC 
ATCTACATTT CGCACGTCGA GCCGGGATCA AAAGCCCAGG ATGTGGGCCT TAAGCGAGGC 
GATCAGATTC ACGAAGTAAA TGGACAGTCG CTGGACCATG TGACTAGCAA GCGAGCCCTC 
GAGATACTCA CAGGCACCAC CCATTTGAGC ATCAGCGTTA AGAGCAACTT GCTTGGCTTC 
AAGGAGATTA TGCAGGCTCT TGAGCATGGC GGTGGAACCG CTGGATCCGG AAGCATTTCC 
GCAGGCAGCG GCAGTTTCAA GTCCGTGCGA AGTCCACGCC GTATTTGCGC CAATGATATT 
GCTAAGTTGC ACGGTCGCTC TGATAGCACC ACCGACGAAT TGTCCAGCGT CAGCGCCTCG 
AATCGGGCGC ACATGGTGCG CCTTAGTTCC GTCGATATGC TACTTGACCA GCCGGACTGC 
GCTCCACCGC AAACGCCGCC AGTGAGTGGG AGCGGTAACA TGGCCTCCAA TTTTATGCAG 
CAACTCTTGC AAAGCGTTAA CAATAGTTCG GCCAAGAAGT CCGGTGGGAA TTCAAACTCG 
GATCAGCAAG ATACGAAGGG CGGTTTTATG ACTCTGGCGC CAAAGCGTCG GTTGCAGAAA 
GCGCTGGCAA AGATGAATTT GCTCAATAAG CAAAATCACG GCAGCAGCCT AAACGACTCC 
TCGGATACGC TGCTCAACGA TCCCAAGTCA AAACTTTCTG CAGTTAGCTC GTGCAGCAGT 
TCCACTCAGT CGTCTATCAA TGGATGCACG GTCAGTGGGG GTGGCCGGCT CTACCAGTCG 
CAATCGAATC CGGATTTAAC CAGTCTCAAC TATGACGGAG GCAGCGATGC GGGTGGAAAC 
GGTGGTGGAA GATTGCAAGT GAACTACCTG AACGCCCACA TTCACCGGCC ATCAGCGGCC 
AGTACTTTGA CAACAAACTC GACGCAATCG CACCTCTTGC CCGATTACCC GGACCACGTG 
CTCAAGGTGT ACAAGGCAGA CCAGACGTGC AAGTATGTAC TCATCTACAA GGAGACGACA 
GCCCACGAGG TCGTAATGCT GACGCTGCAG GAGTTTGGAA TACACGACCC CAGCTCGAAT 
TTCTCGCTAT GCGAAGTGAG TGTTGGCGAC GGGGGTATGG TAAAGCAGCG CCGGTTGCCC 
GATCAGCTGC AGAACCTGGC CGAGCGAATA AGCTTTGCGG CGCGTTACTA CCTCAAGTTA 
AACGATAGCA CCGAACCGCT GGTTCCAGAC GAACTGGCCC TGGAACTGGT ACGCGAGTCG 
AACGTGCACT TCCTGCACCT GAATGCATAC GAGCTGGCCA TTCAGCTCAC TCTACAGGAC 
TTTGCGAACT TTCGCCAGAT TGAGTCCACC GAGTACGTGG ACGAATTGTT CGAGTTGCGC 
TCACGCTACG GAGTGCCAAT GCTGAGCAAG TTTGCCGAAC TGGTCAATCG TGAAATGTTT 
TGGGTTGTGA GTGAGATTTG CGCAGAGCAC AACATTGTGC GCCGCATGAA GATCGTCAAG 
CAGTTCATCA AAATTGCGCG CCACTGCAAG GAGTGCCGTA ACTTTAACTC CATGTTTGCA 
ATCGTCTCGG GGCTTGGACA CGGAGCTGTT TCGCGACTGC GTCAAACGTG GGAGAAGCTG 
CCCTCCAAAT ATCAGCGACT GTTCAACGAC CTGCAGGATC TGATGGATCC CTCGCGCAAC 
ATGAGCAAGT ATCGGCAACT AGTGTCCGCC GAACTACTGG CCCAGCACCC CATCATCCCG 
TTCTATCCGA TCGTCAAGAA GGATCTCACC TTTATTCACC TGGGCAATGA TACGAGAGTT 
GATGGCCTAA TCAACTTTGA GAAGCTGCGA ATGCTGGCCA AGGAGGTACG CCTGCTCACG 
CACATGTGCA GTTCGCCATA CGATCTGCTG TCGATCCTTG AGCTCAAGGG GCAGTCGCCA 
TCCAACGCTC TCTTCTCGCT CAATCAGATG TCTGCATCGC AGAGCAACGC TGCCGCTGGC 
ACAGTTATCG CCGCCAATGC CGGCCAGGCG ACTATCAAGC GGCGCAAGAA GTCGACGGCG 
GCGCCCAATC CCAAGAAGAT GTTTGAGGAG GCGCAGATGG TGCGGCGGGT GAAAGCATAT 
CTCAACAGCC TCAAAATACT CAGTGACGAG GACCTTTTGC ACAAATTCTC GCTGGAATGC 
GAGCCCGCGC ACGGGTCCAC GTACTCGGGC AGCATCTCGC ACGGAAATAC CTCACACCGA 
AGCGGTGGTG GAGGCAGCAT CAGTGGCGGT GCTGGTGGCA GCTCCGGTGG TGGTGGCGGG 
GGCAGTTCCA GCCTCAATGC CGGCGACCAA CTGAGCATCT ATTCGCATAC CAGCTCCAGC 
TCGGCGCCGA ACTCCTCACT ATCGCTTCGC AAGAGGCACC CAAGTTCACC CACCCTTTCG 
ACTACCAGCT CAACGAGTTC TACCAGCGAT CATCAAAGAA GACAGATGCA TAACAACGGC 
CCAAAGTTTG GCACAGCCTC GCCACAAGCA GTTAAAAAGA TGCTATCGCT GTCAGAGTCC 
TCCAAGATTC GGCCGCACCA GCCATTCGTG CCGCGTCACG GATCCACAAT GGCCGGAGTG 
ATTCCACCAC TGCATCACAT GCATGCGGCA CATGGTTTCA GCACACCGTC GCCTGGAGGA 
GTGGTCACCT CACCGGCAAC CAGTGCAGTG GCCAATGTTC AGTGTACGCC AAGTCCTAGT 
CCCTGCTCCC ACCGACGACT GGCTTCTGGA GGCAACATTA TTCCCTCTCG CGCAATACAC 
GAGCGGTCGC ACTCGGACAC ACCAGCTCCG CCGCCGCCTC TACCCTCGGT CGATCTCTCC 
CTAGAGAGCA GTAGTGTAAC TACATTTCGC GATTTACCAT TGCGCAAATC CGTGACTTCC 
GGTTCCATAT CGTCCTGCGA CAGCGGCTAC GTGCATCAGC AGCAGCATTA CCACTTGCAG 
TACCAGCAGC AGCAACAACA GAACAGCTCG CAACACGAGC CATCCCCGCC GGTCTATACC 
GCAGCCGACT GCCGGCTGCT TCAGCAGATA TCGAACAACG CGGTGACCCG CAACTTGAAC 
AGTCCCTGTC AAAGCACAAA CACACCACCC AGCACACCTA CACCTCCACC GAACCAGCCC 
ACAGCAACCA TTCAGCTTTC TGCACCGCCG ACGGCAGCAG CATACATGCA CGCACGCAGC 
CAGCATCAGC AGCTCCAGCA GCAGCAGCAA TCTTTGGCCA TGCCACCACC GCCGCCACCG 
CCGTACAATG TGCCCCCCCT GGGCAGTATC TACAGCCACC ACCAGGGCAC TGCTGGCAGC 
AGGCACCTGA ACCACATGCA TGGTGGCCCG CCAAATTTTA TGGATAGCAC CAAGTGTACC 
ATATGTCCCA TGCCACCCAT GAATCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260126.1
CDS	323..5032
Translation	MDPYHHIRHHYPPTSIAGTGVVVGSSTTINRPELHQKCNRGSHSSDTSSAYSGSDTMASNYASSLEAEEIDLSGLVESVVDSDEEDLAESMDSLTVRDAVRDCLEKDPAERSEEDVEVLLEFTQGLKAFTNITLAVRRALCSVMVFAVVDKAGTVVMSDGEELDSWSVLINGAVEIEHANGSREELQMGDSFGILPTMDKLYHRGVMRTKCDDCQFVCITQTDYYRIQHQGEENTRRHEDENGFVVMVTELRSIGGAGTDSAGSGGSATGASASLNMKRGHVVIRGTPERLLQQLVEENSMTDPTYVEDFLLTHRIFIQNPQEVTSKLLHWFDLEQVDAHKTQELRDRVTRVVLLWVNNHFTDFEADYEMMEFLEVFEALLERKKLLSQLRLLHIACAAKARMRSCTLTRSSRDEPLNFRIVGGYELRGVAIATGNAAVGIYISHVEPGSKAQDVGLKRGDQIHEVNGQSLDHVTSKRALEILTGTTHLSISVKSNLLGFKEIMQALEHGGGTAGSGSISAGSGSFKSVRSPRRICANDIAKLHGRSDSTTDELSSVSASNRAHMVRLSSVDMLLDQPDCAPPQTPPVSGSGNMASNFMQQLLQSVNNSSAKKSGGNSNSDQQDTKGGFMTLAPKRRLQKALAKMNLLNKQNHGSSLNDSSDTLLNDPKSKLSAVSSCSSSTQSSINGCTVSGGGRLYQSQSNPDLTSLNYDGGSDAGGNGGGRLQVNYLNAHIHRPSAASTLTTNSTQSHLLPDYPDHVLKVYKADQTCKYVLIYKETTAHEVVMLTLQEFGIHDPSSNFSLCEVSVGDGGMVKQRRLPDQLQNLAERISFAARYYLKLNDSTEPLVPDELALELVRESNVHFLHLNAYELAIQLTLQDFANFRQIESTEYVDELFELRSRYGVPMLSKFAELVNREMFWVVSEICAEHNIVRRMKIVKQFIKIARHCKECRNFNSMFAIVSGLGHGAVSRLRQTWEKLPSKYQRLFNDLQDLMDPSRNMSKYRQLVSAELLAQHPIIPFYPIVKKDLTFIHLGNDTRVDGLINFEKLRMLAKEVRLLTHMCSSPYDLLSILELKGQSPSNALFSLNQMSASQSNAAAGTVIAANAGQATIKRRKKSTAAPNPKKMFEEAQMVRRVKAYLNSLKILSDEDLLHKFSLECEPAHGSTYSGSISHGNTSHRSGGGGSISGGAGGSSGGGGGGSSSLNAGDQLSIYSHTSSSSAPNSSLSLRKRHPSSPTLSTTSSTSSTSDHQRRQMHNNGPKFGTASPQAVKKMLSLSESSKIRPHQPFVPRHGSTMAGVIPPLHHMHAAHGFSTPSPGGVVTSPATSAVANVQCTPSPSPCSHRRLASGGNIIPSRAIHERSHSDTPAPPPPLPSVDLSLESSSVTTFRDLPLRKSVTSGSISSCDSGYVHQQQHYHLQYQQQQQQNSSQHEPSPPVYTAADCRLLQQISNNAVTRNLNSPCQSTNTPPSTPTPPPNQPTATIQLSAPPTAAAYMHARSQHQQLQQQQQSLAMPPPPPPPYNVPPLGSIYSHHQGTAGSRHLNHMHGGPPNFMDSTKCTICPMPPMNQ

Target ORF information:

RefSeq Version	NM_001273197.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PDZ domain-containing guanine nucleotide exchange factor (PDZ-GEF), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273197.1

ORF Insert Sequence:

ATGGATCCGT ATCACCATAT CAGACATCAT TATCCACCCA CAAGCATAGC AGGCACAGGA 
GTGGTGGTGG GCAGCTCGAC TACAATCAAC CGGCCGGAAC TGCACCAAAA ATGCAACCGG 
GGCTCGCACT CCAGTGACAC CAGTTCGGCG TACAGCGGCA GCGATACAAT GGCCTCGAAC 
TATGCCTCGT CGCTGGAGGC CGAGGAGATT GACCTCTCCG GCCTGGTGGA GTCCGTTGTG 
GACTCTGACG AGGAGGACCT GGCGGAGAGC ATGGATAGTC TTACCGTGCG GGATGCCGTG 
CGTGACTGTC TGGAAAAGGA TCCGGCGGAG CGCAGCGAGG AAGATGTGGA AGTACTGCTT 
GAGTTCACGC AGGGCCTTAA GGCATTCACA AACATTACGC TGGCAGTGCG AAGAGCACTA 
TGTTCCGTGA TGGTATTCGC CGTTGTGGAC AAGGCGGGAA CCGTGGTCAT GTCAGATGGT 
GAGGAACTGG ACTCATGGTC GGTGCTCATA AACGGAGCGG TGGAGATCGA GCATGCAAAT 
GGCAGTCGCG AAGAGCTGCA GATGGGAGAC TCCTTCGGCA TTCTACCCAC CATGGACAAG 
CTGTACCACC GGGGTGTGAT GCGTACGAAG TGCGATGACT GCCAATTTGT GTGCATTACG 
CAGACGGACT ACTACCGTAT TCAGCATCAA GGCGAGGAGA ATACCCGGCG ACATGAGGAC 
GAGAATGGTT TCGTTGTTAT GGTCACTGAA CTCCGGTCCA TTGGCGGCGC CGGCACTGAT 
TCTGCTGGCA GTGGCGGATC GGCGACAGGA GCTTCGGCTT CGCTAAATAT GAAGCGTGGT 
CACGTAGTTA TCCGCGGTAC TCCAGAGCGT CTGCTACAGC AGCTGGTCGA GGAAAACTCG 
ATGACCGATC CCACATACGT GGAGGACTTT TTGCTCACAC ACCGCATCTT TATTCAGAAC 
CCACAAGAGG TGACCAGCAA GTTGCTGCAT TGGTTTGATT TGGAGCAGGT GGATGCGCAC 
AAGACGCAGG AACTGCGAGA TCGCGTAACA CGCGTCGTGC TCCTCTGGGT GAACAACCAT 
TTCACCGATT TCGAGGCGGA CTATGAGATG ATGGAGTTTC TGGAAGTCTT TGAGGCACTG 
TTAGAGCGAA AAAAGTTGCT AAGTCAGCTG CGCCTGTTGC ACATAGCCTG TGCCGCCAAA 
GCACGGATGC GCAGCTGTAC CCTCACTCGA TCGTCACGTG ACGAGCCGCT CAACTTCCGC 
ATCGTGGGCG GCTACGAGCT TCGAGGCGTC GCCATAGCCA CTGGAAACGC AGCAGTGGGC 
ATCTACATTT CGCACGTCGA GCCGGGATCA AAAGCCCAGG ATGTGGGCCT TAAGCGAGGC 
GATCAGATTC ACGAAGTAAA TGGACAGTCG CTGGACCATG TGACTAGCAA GCGAGCCCTC 
GAGATACTCA CAGGCACCAC CCATTTGAGC ATCAGCGTTA AGAGCAACTT GCTTGGCTTC 
AAGGAGATTA TGCAGGCTCT TGAGCATGGC GGTGGAACCG CTGGATCCGG AAGCATTTCC 
GCAGGCAGCG GCAGTTTCAA GTCCGTGCGA AGTCCACGCC GTATTTGCGC CAATGATATT 
GCTAAGTTGC ACGGTCGCTC TGATAGCACC ACCGACGAAT TGTCCAGCGT CAGCGCCTCG 
AATCGGGCGC ACATGGTGCG CCTTAGTTCC GTCGATATGC TACTTGACCA GCCGGACTGC 
GCTCCACCGC AAACGCCGCC AGTGAGTGGG AGCGGTAACA TGGCCTCCAA TTTTATGCAG 
CAACTCTTGC AAAGCGTTAA CAATAGTTCG GCCAAGAAGT CCGGTGGGAA TTCAAACTCG 
GATCAGCAAG ATACGAAGGG CGGTTTTATG ACTCTGGCGC CAAAGCGTCG GTTGCAGAAA 
GCGCTGGCAA AGATGAATTT GCTCAATAAG CAAAATCACG GCAGCAGCCT AAACGACTCC 
TCGGATACGC TGCTCAACGA TCCCAAGTCA AAACTTTCTG CAGTTAGCTC GTGCAGCAGT 
TCCACTCAGT CGTCTATCAA TGGATGCACG GTCAGTGGGG GTGGCCGGCT CTACCAGTCG 
CAATCGAATC CGGATTTAAC CAGTCTCAAC TATGACGGAG GCAGCGATGC GGGTGGAAAC 
GGTGGTGGAA GATTGCAAGT GAACTACCTG AACGCCCACA TTCACCGGCC ATCAGCGGCC 
AGTACTTTGA CAACAAACTC GACGCAATCG CACCTCTTGC CCGATTACCC GGACCACGTG 
CTCAAGGTGT ACAAGGCAGA CCAGACGTGC AAGTATGTAC TCATCTACAA GGAGACGACA 
GCCCACGAGG TCGTAATGCT GACGCTGCAG GAGTTTGGAA TACACGACCC CAGCTCGAAT 
TTCTCGCTAT GCGAAGTGAG TGTTGGCGAC GGGGGTATGG TAAAGCAGCG CCGGTTGCCC 
GATCAGCTGC AGAACCTGGC CGAGCGAATA AGCTTTGCGG CGCGTTACTA CCTCAAGTTA 
AACGATAGCA CCGAACCGCT GGTTCCAGAC GAACTGGCCC TGGAACTGGT ACGCGAGTCG 
AACGTGCACT TCCTGCACCT GAATGCATAC GAGCTGGCCA TTCAGCTCAC TCTACAGGAC 
TTTGCGAACT TTCGCCAGAT TGAGTCCACC GAGTACGTGG ACGAATTGTT CGAGTTGCGC 
TCACGCTACG GAGTGCCAAT GCTGAGCAAG TTTGCCGAAC TGGTCAATCG TGAAATGTTT 
TGGGTTGTGA GTGAGATTTG CGCAGAGCAC AACATTGTGC GCCGCATGAA GATCGTCAAG 
CAGTTCATCA AAATTGCGCG CCACTGCAAG GAGTGCCGTA ACTTTAACTC CATGTTTGCA 
ATCGTCTCGG GGCTTGGACA CGGAGCTGTT TCGCGACTGC GTCAAACGTG GGAGAAGCTG 
CCCTCCAAAT ATCAGCGACT GTTCAACGAC CTGCAGGATC TGATGGATCC CTCGCGCAAC 
ATGAGCAAGT ATCGGCAACT AGTGTCCGCC GAACTACTGG CCCAGCACCC CATCATCCCG 
TTCTATCCGA TCGTCAAGAA GGATCTCACC TTTATTCACC TGGGCAATGA TACGAGAGTT 
GATGGCCTAA TCAACTTTGA GAAGCTGCGA ATGCTGGCCA AGGAGGTACG CCTGCTCACG 
CACATGTGCA GTTCGCCATA CGATCTGCTG TCGATCCTTG AGCTCAAGGG GCAGTCGCCA 
TCCAACGCTC TCTTCTCGCT CAATCAGATG TCTGCATCGC AGAGCAACGC TGCCGCTGGC 
ACAGTTATCG CCGCCAATGC CGGCCAGGCG ACTATCAAGC GGCGCAAGAA GTCGACGGCG 
GCGCCCAATC CCAAGAAGAT GTTTGAGGAG GCGCAGATGG TGCGGCGGGT GAAAGCATAT 
CTCAACAGCC TCAAAATACT CAGTGACGAG GACCTTTTGC ACAAATTCTC GCTGGAATGC 
GAGCCCGCGC ACGGGTCCAC GTACTCGGGC AGCATCTCGC ACGGAAATAC CTCACACCGA 
AGCGGTGGTG GAGGCAGCAT CAGTGGCGGT GCTGGTGGCA GCTCCGGTGG TGGTGGCGGG 
GGCAGTTCCA GCCTCAATGC CGGCGACCAA CTGAGCATCT ATTCGCATAC CAGCTCCAGC 
TCGGCGCCGA ACTCCTCACT ATCGCTTCGC AAGAGGCACC CAAGTTCACC CACCCTTTCG 
ACTACCAGCT CAACGAGTTC TACCAGCGAT CATCAAAGAA GACAGATGCA TAACAACGGC 
CCAAAGTTTG GCACAGCCTC GCCACAAGCA GTTAAAAAGA TGCTATCGCT GTCAGAGTCC 
TCCAAGATTC GGCCGCACCA GCCATTCGTG CCGCGTCACG GATCCACAAT GGCCGGAGTG 
ATTCCACCAC TGCATCACAT GCATGCGGCA CATGGTTTCA GCACACCGTC GCCTGGAGGA 
GTGGTCACCT CACCGGCAAC CAGTGCAGTG GCCAATGTTC AGTGTACGCC AAGTCCTAGT 
CCCTGCTCCC ACCGACGACT GGCTTCTGGA GGCAACATTA TTCCCTCTCG CGCAATACAC 
GAGCGGTCGC ACTCGGACAC ACCAGCTCCG CCGCCGCCTC TACCCTCGGT CGATCTCTCC 
CTAGAGAGCA GTAGTGTAAC TACATTTCGC GATTTACCAT TGCGCAAATC CGTGACTTCC 
GGTTCCATAT CGTCCTGCGA CAGCGGCTAC GTGCATCAGC AGCAGCATTA CCACTTGCAG 
TACCAGCAGC AGCAACAACA GAACAGCTCG CAACACGAGC CATCCCCGCC GGTCTATACC 
GCAGCCGACT GCCGGCTGCT TCAGCAGATA TCGAACAACG CGGTGACCCG CAACTTGAAC 
AGTCCCTGTC AAAGCACAAA CACACCACCC AGCACACCTA CACCTCCACC GAACCAGCCC 
ACAGCAACCA TTCAGCTTTC TGCACCGCCG ACGGCAGCAG CATACATGCA CGCACGCAGC 
CAGCATCAGC AGCTCCAGCA GCAGCAGCAA TCTTTGGCCA TGCCACCACC GCCGCCACCG 
CCGTACAATG TGCCCCCCCT GGGCAGTATC TACAGCCACC ACCAGGGCAC TGCTGGCAGC 
AGGCACCTGA ACCACATGCA TGGTGGCCCG CCAAATTTTA TGGATAGCAC CAAGTGTACC 
ATATGTCCCA TGCCACCCAT GAATCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDZ-GEF ( NM_001144325.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137797.1 Drosophila melanogaster PDZ domain-containing guanine nucleotide exchange factor (PDZ-GEF), transcript variant B, mRNA.

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CloneID	OFa15801
Clone ID Related Accession (Same CDS sequence)	NM_001273197.1 , NM_001144325.2
Accession Version	NM_001144325.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PDZ domain-containing guanine nucleotide exchange factor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001144325.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCCGT ATCACCATAT CAGACATCAT TATCCACCCA CAAGCATAGC AGGCACAGGA 
GTGGTGGTGG GCAGCTCGAC TACAATCAAC CGGCCGGAAC TGCACCAAAA ATGCAACCGG 
GGCTCGCACT CCAGTGACAC CAGTTCGGCG TACAGCGGCA GCGATACAAT GGCCTCGAAC 
TATGCCTCGT CGCTGGAGGC CGAGGAGATT GACCTCTCCG GCCTGGTGGA GTCCGTTGTG 
GACTCTGACG AGGAGGACCT GGCGGAGAGC ATGGATAGTC TTACCGTGCG GGATGCCGTG 
CGTGACTGTC TGGAAAAGGA TCCGGCGGAG CGCAGCGAGG AAGATGTGGA AGTACTGCTT 
GAGTTCACGC AGGGCCTTAA GGCATTCACA AACATTACGC TGGCAGTGCG AAGAGCACTA 
TGTTCCGTGA TGGTATTCGC CGTTGTGGAC AAGGCGGGAA CCGTGGTCAT GTCAGATGGT 
GAGGAACTGG ACTCATGGTC GGTGCTCATA AACGGAGCGG TGGAGATCGA GCATGCAAAT 
GGCAGTCGCG AAGAGCTGCA GATGGGAGAC TCCTTCGGCA TTCTACCCAC CATGGACAAG 
CTGTACCACC GGGGTGTGAT GCGTACGAAG TGCGATGACT GCCAATTTGT GTGCATTACG 
CAGACGGACT ACTACCGTAT TCAGCATCAA GGCGAGGAGA ATACCCGGCG ACATGAGGAC 
GAGAATGGTT TCGTTGTTAT GGTCACTGAA CTCCGGTCCA TTGGCGGCGC CGGCACTGAT 
TCTGCTGGCA GTGGCGGATC GGCGACAGGA GCTTCGGCTT CGCTAAATAT GAAGCGTGGT 
CACGTAGTTA TCCGCGGTAC TCCAGAGCGT CTGCTACAGC AGCTGGTCGA GGAAAACTCG 
ATGACCGATC CCACATACGT GGAGGACTTT TTGCTCACAC ACCGCATCTT TATTCAGAAC 
CCACAAGAGG TGACCAGCAA GTTGCTGCAT TGGTTTGATT TGGAGCAGGT GGATGCGCAC 
AAGACGCAGG AACTGCGAGA TCGCGTAACA CGCGTCGTGC TCCTCTGGGT GAACAACCAT 
TTCACCGATT TCGAGGCGGA CTATGAGATG ATGGAGTTTC TGGAAGTCTT TGAGGCACTG 
TTAGAGCGAA AAAAGTTGCT AAGTCAGCTG CGCCTGTTGC ACATAGCCTG TGCCGCCAAA 
GCACGGATGC GCAGCTGTAC CCTCACTCGA TCGTCACGTG ACGAGCCGCT CAACTTCCGC 
ATCGTGGGCG GCTACGAGCT TCGAGGCGTC GCCATAGCCA CTGGAAACGC AGCAGTGGGC 
ATCTACATTT CGCACGTCGA GCCGGGATCA AAAGCCCAGG ATGTGGGCCT TAAGCGAGGC 
GATCAGATTC ACGAAGTAAA TGGACAGTCG CTGGACCATG TGACTAGCAA GCGAGCCCTC 
GAGATACTCA CAGGCACCAC CCATTTGAGC ATCAGCGTTA AGAGCAACTT GCTTGGCTTC 
AAGGAGATTA TGCAGGCTCT TGAGCATGGC GGTGGAACCG CTGGATCCGG AAGCATTTCC 
GCAGGCAGCG GCAGTTTCAA GTCCGTGCGA AGTCCACGCC GTATTTGCGC CAATGATATT 
GCTAAGTTGC ACGGTCGCTC TGATAGCACC ACCGACGAAT TGTCCAGCGT CAGCGCCTCG 
AATCGGGCGC ACATGGTGCG CCTTAGTTCC GTCGATATGC TACTTGACCA GCCGGACTGC 
GCTCCACCGC AAACGCCGCC AGTGAGTGGG AGCGGTAACA TGGCCTCCAA TTTTATGCAG 
CAACTCTTGC AAAGCGTTAA CAATAGTTCG GCCAAGAAGT CCGGTGGGAA TTCAAACTCG 
GATCAGCAAG ATACGAAGGG CGGTTTTATG ACTCTGGCGC CAAAGCGTCG GTTGCAGAAA 
GCGCTGGCAA AGATGAATTT GCTCAATAAG CAAAATCACG GCAGCAGCCT AAACGACTCC 
TCGGATACGC TGCTCAACGA TCCCAAGTCA AAACTTTCTG CAGTTAGCTC GTGCAGCAGT 
TCCACTCAGT CGTCTATCAA TGGATGCACG GTCAGTGGGG GTGGCCGGCT CTACCAGTCG 
CAATCGAATC CGGATTTAAC CAGTCTCAAC TATGACGGAG GCAGCGATGC GGGTGGAAAC 
GGTGGTGGAA GATTGCAAGT GAACTACCTG AACGCCCACA TTCACCGGCC ATCAGCGGCC 
AGTACTTTGA CAACAAACTC GACGCAATCG CACCTCTTGC CCGATTACCC GGACCACGTG 
CTCAAGGTGT ACAAGGCAGA CCAGACGTGC AAGTATGTAC TCATCTACAA GGAGACGACA 
GCCCACGAGG TCGTAATGCT GACGCTGCAG GAGTTTGGAA TACACGACCC CAGCTCGAAT 
TTCTCGCTAT GCGAAGTGAG TGTTGGCGAC GGGGGTATGG TAAAGCAGCG CCGGTTGCCC 
GATCAGCTGC AGAACCTGGC CGAGCGAATA AGCTTTGCGG CGCGTTACTA CCTCAAGTTA 
AACGATAGCA CCGAACCGCT GGTTCCAGAC GAACTGGCCC TGGAACTGGT ACGCGAGTCG 
AACGTGCACT TCCTGCACCT GAATGCATAC GAGCTGGCCA TTCAGCTCAC TCTACAGGAC 
TTTGCGAACT TTCGCCAGAT TGAGTCCACC GAGTACGTGG ACGAATTGTT CGAGTTGCGC 
TCACGCTACG GAGTGCCAAT GCTGAGCAAG TTTGCCGAAC TGGTCAATCG TGAAATGTTT 
TGGGTTGTGA GTGAGATTTG CGCAGAGCAC AACATTGTGC GCCGCATGAA GATCGTCAAG 
CAGTTCATCA AAATTGCGCG CCACTGCAAG GAGTGCCGTA ACTTTAACTC CATGTTTGCA 
ATCGTCTCGG GGCTTGGACA CGGAGCTGTT TCGCGACTGC GTCAAACGTG GGAGAAGCTG 
CCCTCCAAAT ATCAGCGACT GTTCAACGAC CTGCAGGATC TGATGGATCC CTCGCGCAAC 
ATGAGCAAGT ATCGGCAACT AGTGTCCGCC GAACTACTGG CCCAGCACCC CATCATCCCG 
TTCTATCCGA TCGTCAAGAA GGATCTCACC TTTATTCACC TGGGCAATGA TACGAGAGTT 
GATGGCCTAA TCAACTTTGA GAAGCTGCGA ATGCTGGCCA AGGAGGTACG CCTGCTCACG 
CACATGTGCA GTTCGCCATA CGATCTGCTG TCGATCCTTG AGCTCAAGGG GCAGTCGCCA 
TCCAACGCTC TCTTCTCGCT CAATCAGATG TCTGCATCGC AGAGCAACGC TGCCGCTGGC 
ACAGTTATCG CCGCCAATGC CGGCCAGGCG ACTATCAAGC GGCGCAAGAA GTCGACGGCG 
GCGCCCAATC CCAAGAAGAT GTTTGAGGAG GCGCAGATGG TGCGGCGGGT GAAAGCATAT 
CTCAACAGCC TCAAAATACT CAGTGACGAG GACCTTTTGC ACAAATTCTC GCTGGAATGC 
GAGCCCGCGC ACGGGTCCAC GTACTCGGGC AGCATCTCGC ACGGAAATAC CTCACACCGA 
AGCGGTGGTG GAGGCAGCAT CAGTGGCGGT GCTGGTGGCA GCTCCGGTGG TGGTGGCGGG 
GGCAGTTCCA GCCTCAATGC CGGCGACCAA CTGAGCATCT ATTCGCATAC CAGCTCCAGC 
TCGGCGCCGA ACTCCTCACT ATCGCTTCGC AAGAGGCACC CAAGTTCACC CACCCTTTCG 
ACTACCAGCT CAACGAGTTC TACCAGCGAT CATCAAAGAA GACAGATGCA TAACAACGGC 
CCAAAGTTTG GCACAGCCTC GCCACAAGCA GTTAAAAAGA TGCTATCGCT GTCAGAGTCC 
TCCAAGATTC GGCCGCACCA GCCATTCGTG CCGCGTCACG GATCCACAAT GGCCGGAGTG 
ATTCCACCAC TGCATCACAT GCATGCGGCA CATGGTTTCA GCACACCGTC GCCTGGAGGA 
GTGGTCACCT CACCGGCAAC CAGTGCAGTG GCCAATGTTC AGTGTACGCC AAGTCCTAGT 
CCCTGCTCCC ACCGACGACT GGCTTCTGGA GGCAACATTA TTCCCTCTCG CGCAATACAC 
GAGCGGTCGC ACTCGGACAC ACCAGCTCCG CCGCCGCCTC TACCCTCGGT CGATCTCTCC 
CTAGAGAGCA GTAGTGTAAC TACATTTCGC GATTTACCAT TGCGCAAATC CGTGACTTCC 
GGTTCCATAT CGTCCTGCGA CAGCGGCTAC GTGCATCAGC AGCAGCATTA CCACTTGCAG 
TACCAGCAGC AGCAACAACA GAACAGCTCG CAACACGAGC CATCCCCGCC GGTCTATACC 
GCAGCCGACT GCCGGCTGCT TCAGCAGATA TCGAACAACG CGGTGACCCG CAACTTGAAC 
AGTCCCTGTC AAAGCACAAA CACACCACCC AGCACACCTA CACCTCCACC GAACCAGCCC 
ACAGCAACCA TTCAGCTTTC TGCACCGCCG ACGGCAGCAG CATACATGCA CGCACGCAGC 
CAGCATCAGC AGCTCCAGCA GCAGCAGCAA TCTTTGGCCA TGCCACCACC GCCGCCACCG 
CCGTACAATG TGCCCCCCCT GGGCAGTATC TACAGCCACC ACCAGGGCAC TGCTGGCAGC 
AGGCACCTGA ACCACATGCA TGGTGGCCCG CCAAATTTTA TGGATAGCAC CAAGTGTACC 
ATATGTCCCA TGCCACCCAT GAATCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137797.1
CDS	448..5157
Translation	MDPYHHIRHHYPPTSIAGTGVVVGSSTTINRPELHQKCNRGSHSSDTSSAYSGSDTMASNYASSLEAEEIDLSGLVESVVDSDEEDLAESMDSLTVRDAVRDCLEKDPAERSEEDVEVLLEFTQGLKAFTNITLAVRRALCSVMVFAVVDKAGTVVMSDGEELDSWSVLINGAVEIEHANGSREELQMGDSFGILPTMDKLYHRGVMRTKCDDCQFVCITQTDYYRIQHQGEENTRRHEDENGFVVMVTELRSIGGAGTDSAGSGGSATGASASLNMKRGHVVIRGTPERLLQQLVEENSMTDPTYVEDFLLTHRIFIQNPQEVTSKLLHWFDLEQVDAHKTQELRDRVTRVVLLWVNNHFTDFEADYEMMEFLEVFEALLERKKLLSQLRLLHIACAAKARMRSCTLTRSSRDEPLNFRIVGGYELRGVAIATGNAAVGIYISHVEPGSKAQDVGLKRGDQIHEVNGQSLDHVTSKRALEILTGTTHLSISVKSNLLGFKEIMQALEHGGGTAGSGSISAGSGSFKSVRSPRRICANDIAKLHGRSDSTTDELSSVSASNRAHMVRLSSVDMLLDQPDCAPPQTPPVSGSGNMASNFMQQLLQSVNNSSAKKSGGNSNSDQQDTKGGFMTLAPKRRLQKALAKMNLLNKQNHGSSLNDSSDTLLNDPKSKLSAVSSCSSSTQSSINGCTVSGGGRLYQSQSNPDLTSLNYDGGSDAGGNGGGRLQVNYLNAHIHRPSAASTLTTNSTQSHLLPDYPDHVLKVYKADQTCKYVLIYKETTAHEVVMLTLQEFGIHDPSSNFSLCEVSVGDGGMVKQRRLPDQLQNLAERISFAARYYLKLNDSTEPLVPDELALELVRESNVHFLHLNAYELAIQLTLQDFANFRQIESTEYVDELFELRSRYGVPMLSKFAELVNREMFWVVSEICAEHNIVRRMKIVKQFIKIARHCKECRNFNSMFAIVSGLGHGAVSRLRQTWEKLPSKYQRLFNDLQDLMDPSRNMSKYRQLVSAELLAQHPIIPFYPIVKKDLTFIHLGNDTRVDGLINFEKLRMLAKEVRLLTHMCSSPYDLLSILELKGQSPSNALFSLNQMSASQSNAAAGTVIAANAGQATIKRRKKSTAAPNPKKMFEEAQMVRRVKAYLNSLKILSDEDLLHKFSLECEPAHGSTYSGSISHGNTSHRSGGGGSISGGAGGSSGGGGGGSSSLNAGDQLSIYSHTSSSSAPNSSLSLRKRHPSSPTLSTTSSTSSTSDHQRRQMHNNGPKFGTASPQAVKKMLSLSESSKIRPHQPFVPRHGSTMAGVIPPLHHMHAAHGFSTPSPGGVVTSPATSAVANVQCTPSPSPCSHRRLASGGNIIPSRAIHERSHSDTPAPPPPLPSVDLSLESSSVTTFRDLPLRKSVTSGSISSCDSGYVHQQQHYHLQYQQQQQQNSSQHEPSPPVYTAADCRLLQQISNNAVTRNLNSPCQSTNTPPSTPTPPPNQPTATIQLSAPPTAAAYMHARSQHQQLQQQQQSLAMPPPPPPPYNVPPLGSIYSHHQGTAGSRHLNHMHGGPPNFMDSTKCTICPMPPMNQ

Target ORF information:

RefSeq Version	NM_001144325.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PDZ domain-containing guanine nucleotide exchange factor (PDZ-GEF), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144325.2

ORF Insert Sequence:

ATGGATCCGT ATCACCATAT CAGACATCAT TATCCACCCA CAAGCATAGC AGGCACAGGA 
GTGGTGGTGG GCAGCTCGAC TACAATCAAC CGGCCGGAAC TGCACCAAAA ATGCAACCGG 
GGCTCGCACT CCAGTGACAC CAGTTCGGCG TACAGCGGCA GCGATACAAT GGCCTCGAAC 
TATGCCTCGT CGCTGGAGGC CGAGGAGATT GACCTCTCCG GCCTGGTGGA GTCCGTTGTG 
GACTCTGACG AGGAGGACCT GGCGGAGAGC ATGGATAGTC TTACCGTGCG GGATGCCGTG 
CGTGACTGTC TGGAAAAGGA TCCGGCGGAG CGCAGCGAGG AAGATGTGGA AGTACTGCTT 
GAGTTCACGC AGGGCCTTAA GGCATTCACA AACATTACGC TGGCAGTGCG AAGAGCACTA 
TGTTCCGTGA TGGTATTCGC CGTTGTGGAC AAGGCGGGAA CCGTGGTCAT GTCAGATGGT 
GAGGAACTGG ACTCATGGTC GGTGCTCATA AACGGAGCGG TGGAGATCGA GCATGCAAAT 
GGCAGTCGCG AAGAGCTGCA GATGGGAGAC TCCTTCGGCA TTCTACCCAC CATGGACAAG 
CTGTACCACC GGGGTGTGAT GCGTACGAAG TGCGATGACT GCCAATTTGT GTGCATTACG 
CAGACGGACT ACTACCGTAT TCAGCATCAA GGCGAGGAGA ATACCCGGCG ACATGAGGAC 
GAGAATGGTT TCGTTGTTAT GGTCACTGAA CTCCGGTCCA TTGGCGGCGC CGGCACTGAT 
TCTGCTGGCA GTGGCGGATC GGCGACAGGA GCTTCGGCTT CGCTAAATAT GAAGCGTGGT 
CACGTAGTTA TCCGCGGTAC TCCAGAGCGT CTGCTACAGC AGCTGGTCGA GGAAAACTCG 
ATGACCGATC CCACATACGT GGAGGACTTT TTGCTCACAC ACCGCATCTT TATTCAGAAC 
CCACAAGAGG TGACCAGCAA GTTGCTGCAT TGGTTTGATT TGGAGCAGGT GGATGCGCAC 
AAGACGCAGG AACTGCGAGA TCGCGTAACA CGCGTCGTGC TCCTCTGGGT GAACAACCAT 
TTCACCGATT TCGAGGCGGA CTATGAGATG ATGGAGTTTC TGGAAGTCTT TGAGGCACTG 
TTAGAGCGAA AAAAGTTGCT AAGTCAGCTG CGCCTGTTGC ACATAGCCTG TGCCGCCAAA 
GCACGGATGC GCAGCTGTAC CCTCACTCGA TCGTCACGTG ACGAGCCGCT CAACTTCCGC 
ATCGTGGGCG GCTACGAGCT TCGAGGCGTC GCCATAGCCA CTGGAAACGC AGCAGTGGGC 
ATCTACATTT CGCACGTCGA GCCGGGATCA AAAGCCCAGG ATGTGGGCCT TAAGCGAGGC 
GATCAGATTC ACGAAGTAAA TGGACAGTCG CTGGACCATG TGACTAGCAA GCGAGCCCTC 
GAGATACTCA CAGGCACCAC CCATTTGAGC ATCAGCGTTA AGAGCAACTT GCTTGGCTTC 
AAGGAGATTA TGCAGGCTCT TGAGCATGGC GGTGGAACCG CTGGATCCGG AAGCATTTCC 
GCAGGCAGCG GCAGTTTCAA GTCCGTGCGA AGTCCACGCC GTATTTGCGC CAATGATATT 
GCTAAGTTGC ACGGTCGCTC TGATAGCACC ACCGACGAAT TGTCCAGCGT CAGCGCCTCG 
AATCGGGCGC ACATGGTGCG CCTTAGTTCC GTCGATATGC TACTTGACCA GCCGGACTGC 
GCTCCACCGC AAACGCCGCC AGTGAGTGGG AGCGGTAACA TGGCCTCCAA TTTTATGCAG 
CAACTCTTGC AAAGCGTTAA CAATAGTTCG GCCAAGAAGT CCGGTGGGAA TTCAAACTCG 
GATCAGCAAG ATACGAAGGG CGGTTTTATG ACTCTGGCGC CAAAGCGTCG GTTGCAGAAA 
GCGCTGGCAA AGATGAATTT GCTCAATAAG CAAAATCACG GCAGCAGCCT AAACGACTCC 
TCGGATACGC TGCTCAACGA TCCCAAGTCA AAACTTTCTG CAGTTAGCTC GTGCAGCAGT 
TCCACTCAGT CGTCTATCAA TGGATGCACG GTCAGTGGGG GTGGCCGGCT CTACCAGTCG 
CAATCGAATC CGGATTTAAC CAGTCTCAAC TATGACGGAG GCAGCGATGC GGGTGGAAAC 
GGTGGTGGAA GATTGCAAGT GAACTACCTG AACGCCCACA TTCACCGGCC ATCAGCGGCC 
AGTACTTTGA CAACAAACTC GACGCAATCG CACCTCTTGC CCGATTACCC GGACCACGTG 
CTCAAGGTGT ACAAGGCAGA CCAGACGTGC AAGTATGTAC TCATCTACAA GGAGACGACA 
GCCCACGAGG TCGTAATGCT GACGCTGCAG GAGTTTGGAA TACACGACCC CAGCTCGAAT 
TTCTCGCTAT GCGAAGTGAG TGTTGGCGAC GGGGGTATGG TAAAGCAGCG CCGGTTGCCC 
GATCAGCTGC AGAACCTGGC CGAGCGAATA AGCTTTGCGG CGCGTTACTA CCTCAAGTTA 
AACGATAGCA CCGAACCGCT GGTTCCAGAC GAACTGGCCC TGGAACTGGT ACGCGAGTCG 
AACGTGCACT TCCTGCACCT GAATGCATAC GAGCTGGCCA TTCAGCTCAC TCTACAGGAC 
TTTGCGAACT TTCGCCAGAT TGAGTCCACC GAGTACGTGG ACGAATTGTT CGAGTTGCGC 
TCACGCTACG GAGTGCCAAT GCTGAGCAAG TTTGCCGAAC TGGTCAATCG TGAAATGTTT 
TGGGTTGTGA GTGAGATTTG CGCAGAGCAC AACATTGTGC GCCGCATGAA GATCGTCAAG 
CAGTTCATCA AAATTGCGCG CCACTGCAAG GAGTGCCGTA ACTTTAACTC CATGTTTGCA 
ATCGTCTCGG GGCTTGGACA CGGAGCTGTT TCGCGACTGC GTCAAACGTG GGAGAAGCTG 
CCCTCCAAAT ATCAGCGACT GTTCAACGAC CTGCAGGATC TGATGGATCC CTCGCGCAAC 
ATGAGCAAGT ATCGGCAACT AGTGTCCGCC GAACTACTGG CCCAGCACCC CATCATCCCG 
TTCTATCCGA TCGTCAAGAA GGATCTCACC TTTATTCACC TGGGCAATGA TACGAGAGTT 
GATGGCCTAA TCAACTTTGA GAAGCTGCGA ATGCTGGCCA AGGAGGTACG CCTGCTCACG 
CACATGTGCA GTTCGCCATA CGATCTGCTG TCGATCCTTG AGCTCAAGGG GCAGTCGCCA 
TCCAACGCTC TCTTCTCGCT CAATCAGATG TCTGCATCGC AGAGCAACGC TGCCGCTGGC 
ACAGTTATCG CCGCCAATGC CGGCCAGGCG ACTATCAAGC GGCGCAAGAA GTCGACGGCG 
GCGCCCAATC CCAAGAAGAT GTTTGAGGAG GCGCAGATGG TGCGGCGGGT GAAAGCATAT 
CTCAACAGCC TCAAAATACT CAGTGACGAG GACCTTTTGC ACAAATTCTC GCTGGAATGC 
GAGCCCGCGC ACGGGTCCAC GTACTCGGGC AGCATCTCGC ACGGAAATAC CTCACACCGA 
AGCGGTGGTG GAGGCAGCAT CAGTGGCGGT GCTGGTGGCA GCTCCGGTGG TGGTGGCGGG 
GGCAGTTCCA GCCTCAATGC CGGCGACCAA CTGAGCATCT ATTCGCATAC CAGCTCCAGC 
TCGGCGCCGA ACTCCTCACT ATCGCTTCGC AAGAGGCACC CAAGTTCACC CACCCTTTCG 
ACTACCAGCT CAACGAGTTC TACCAGCGAT CATCAAAGAA GACAGATGCA TAACAACGGC 
CCAAAGTTTG GCACAGCCTC GCCACAAGCA GTTAAAAAGA TGCTATCGCT GTCAGAGTCC 
TCCAAGATTC GGCCGCACCA GCCATTCGTG CCGCGTCACG GATCCACAAT GGCCGGAGTG 
ATTCCACCAC TGCATCACAT GCATGCGGCA CATGGTTTCA GCACACCGTC GCCTGGAGGA 
GTGGTCACCT CACCGGCAAC CAGTGCAGTG GCCAATGTTC AGTGTACGCC AAGTCCTAGT 
CCCTGCTCCC ACCGACGACT GGCTTCTGGA GGCAACATTA TTCCCTCTCG CGCAATACAC 
GAGCGGTCGC ACTCGGACAC ACCAGCTCCG CCGCCGCCTC TACCCTCGGT CGATCTCTCC 
CTAGAGAGCA GTAGTGTAAC TACATTTCGC GATTTACCAT TGCGCAAATC CGTGACTTCC 
GGTTCCATAT CGTCCTGCGA CAGCGGCTAC GTGCATCAGC AGCAGCATTA CCACTTGCAG 
TACCAGCAGC AGCAACAACA GAACAGCTCG CAACACGAGC CATCCCCGCC GGTCTATACC 
GCAGCCGACT GCCGGCTGCT TCAGCAGATA TCGAACAACG CGGTGACCCG CAACTTGAAC 
AGTCCCTGTC AAAGCACAAA CACACCACCC AGCACACCTA CACCTCCACC GAACCAGCCC 
ACAGCAACCA TTCAGCTTTC TGCACCGCCG ACGGCAGCAG CATACATGCA CGCACGCAGC 
CAGCATCAGC AGCTCCAGCA GCAGCAGCAA TCTTTGGCCA TGCCACCACC GCCGCCACCG 
CCGTACAATG TGCCCCCCCT GGGCAGTATC TACAGCCACC ACCAGGGCAC TGCTGGCAGC 
AGGCACCTGA ACCACATGCA TGGTGGCCCG CCAAATTTTA TGGATAGCAC CAAGTGTACC 
ATATGTCCCA TGCCACCCAT GAATCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PDZ-GEF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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