Kr-h1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kr-h1
Entrez Gene ID	33861
Full Name	Kruppel homolog 1
Synonyms	44/11,CG18783,CG45074,CG9167,Dmel\CG45074,Dmel_CG18783,Kr h,Kr-H1,Kr-h,P381,Pow,l(2)05208,l(2)10642
General protein information	Preferred Names Kruppel homolog 1 Names CG45074 gene product from transcript CG45074-RA CG45074-PA CG45074-PB Kr-h1-PA Kr-h1-PB Krueppel-homolog alpha-isoform Kruppel-homolog 1 Kruppel-homolog-1 kruppel homolog 1 krupple homolog 1 passion of white
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26B5-26B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_058119.5	NP_477467.1	kruppel homolog 1, isoform A
NM_058118.5	NP_477466.1	kruppel homolog 1, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

During photoreceptor differentiation, transient repression of Kr-h1 represents a key step regulating neuronal maturation.
Title: Orthodenticle and Kruppel homolog 1 regulate Drosophila photoreceptor maturation.

KR-H1 has a potential for modulating neuronal morphogenesis, it appears physiologically involved in coordinating general ecdysone signaling.
Title: Roles of Drosophila Kruppel-homolog 1 in neuronal morphogenesis.

At the chromosomal level, KR-H localisation is dynamic and that the protein is recruited to, and released from, loci harbouring an important subset of the known regulatory genes as the response advances.
Title: Dynamic localisation of KR-H during an ecdysone response in Drosophila.

The following Kr-h1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kr-h1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13877	NM_058119.5 Latest version!	Drosophila melanogaster kruppel homolog 1, transcript variant A (Kr-h1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13878	NM_058118.5 Latest version!	Drosophila melanogaster kruppel homolog 1, transcript variant B (Kr-h1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kr-h1 ( NM_058119.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477467.1 Drosophila melanogaster kruppel homolog 1, transcript variant A (Kr-h1), mRNA.

Price & Availability↑

CloneID	OFa13877
Clone ID Related Accession (Same CDS sequence)	NM_058119.5
Accession Version	NM_058119.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kruppel homolog 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_058119.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTACT ATTCCGCCAA CCAGCTGCTT ATAAAAACGG AACAATCTAG TCAGGCGCAG 
TTCTGCCTCC AGGTTCCGCC TCCACTAACA GCGACGACCA CGAGCGTGGG CCTAGGAGTT 
CCGCCATCCG GCGGCCAACA GGAGCACTTC GAGCTGCTGC AGACGCCGCA GCAGCGACAA 
ATGCAACTGC AGCTACAGGA CCAGCACCAG CAGGAGCAGC AGCAGTTTGT CAGCTACCAA 
CTGGCCATCC AGCAGCATCA GAAGCAGCAA CAACAGCAGC AACATGAAAG TATTACGAAC 
GCAGCACCCA CAGCAGCTCC ATCAGCCCAG CGGATCAAGA CAGAGCCCGT CGGCGGATTC 
CCAGCGTCGG CAGCCGTGGT GTCGCAGGTG CGCAAGCCCA GCGCCAGCAA ACCGCAATTC 
AAGTGCGACC AGTGTGGCAT GACCTTTGGC AGCAAGTCCG CCCACACCTC ACACACCAAG 
TCGCACTCGA AGAACCAAGA TCTGTCGCTT AATGGCGCCT CTGGAGCCGG CGTTGCTGCG 
CCCGTCTCAA CCGCGGCCAT CGAGCTCAAT GATGCCGGTC TGCCGGTGGG CATTCCCAAG 
AGCCCCACCA TCAAGCCGCT GGCCAACGTG GCCGCCGGAG CAGATCCCTA TCAGTGCAAT 
GTTTGCCAGA AAACATTCGC CGTACCCGCC AGGCTGATCC GCCACTACCG CACCCACACT 
GGTGAACGGC CATTCGAGTG CGAGTTCTGC CACAAGCTGT TCAGCGTGAA GGAGAACCTC 
CAGGTGCACC GGCGCATCCA CACGAAGGAG CGTCCGTACA AGTGTGACGT CTGTGGACGG 
GCATTCGAAC ACTCCGGGAA GCTGCACCGC CACATGCGCA TTCACACCGG CGAGCGGCCA 
CACAAGTGCT CCGTGTGCGA GAAGACATTC ATCCAGTCCG GCCAGCTGGT GATCCATATG 
CGCACGCACA CCGGCGAGAA GCCGTACAAG TGCCCGGAGC CGGGATGCGG CAAAGGTTTC 
ACCTGCTCCA AGCAGCTCAA GGTGCACTCG CGAACGCACA CGGGCGAGAA GCCCTACCAC 
TGTGACATCT GCTTCCGGGA CTTTGGCTAC AATCATGTGC TGAAACTGCA TCGCGTCCAG 
CACTACGGCT CCAAGTGCTA CAAGTGCACC ATCTGCGACG AGACGTTCAA GAACAAGAAG 
GAGATGGAGG CCCACATCAA GGGCCATGCC AACGAAGTAC CCGACGACGA AGCGGAGGCG 
GCGGCTGCGT CGGCAGCAGC ATCAACGTCC GCAGGCTCCT CCGCCGGATC GCCGTCCTTG 
CAAGGAGTCA GCAGCAACTC CGAGAGCAGC AACCACTCGC CGCCCAGCTC GCCACCGGCC 
ACAAAGAAAC CTCGCCAGGC TCGCCAGCCG CGCGTTTCCA AAACCGTTGC AGCCACCCTT 
TCCATCCCTA CCTCCTCGCC GCTCTCGCCC AGCTCACTCA GCTCCACATA CTCGCCATCG 
GCCAGCAGCA TGGCCTCGCC ACCACCCACA TCGGCCCACT ACCTGCCCGT CCAGATGGAG 
GCGGATGCCT TGAGCCGGGA CAGCGGCGTG TCCAGCGCCC AGCCCGCCCA CAGCACCTAT 
GCAGACGAGG AGCCCACAGA TTTGAGCATG CAACAGGTCC AGGGTCAGCT GCCAGAAAGC 
ACTGTGGACT ACTACCAGGC ACCGCCAAGT CTACTCGAAC TGCAGCCACA ACCCGCTGGT 
CTAACCATCA ATCCTGCCCT GCTTGAAGCG GCCAGCATTG CGCGTCGCCA TGACGACAAC 
GATGACCAGG TGCAGGATGA GGATGTGCAC GCAGCCGCCT GGCAAATGAT GCAGTTGTGC 
CGCGGCCACG GCTCCTTACC CCCAACGGAG CAGCCGGCGC CAAGCCACCA GCCACAGGTG 
CCCACTCTGC ACGTCAGCGA TCTAGCGGCT AACTACGATG ACACCCATGA AGCCACCGTG 
CTGATCGAGC ACTTCAAGCG TGGTGATCTC GCTCGCCACG GGCTGCACAA GGGCTATGCA 
CCAGTGCCCA AATATGAATC CGCTCTACCC AATCCGGACG TTGTGCGACG CGTGGAGGCG 
GCCATCGGCC TGCGTTCCAG CACGGAGTCG CCGGAACGTA GCTCCTCGCC GGAGAGCGAC 
TCCCTGATGA TGGCCGACCG GAACGTGATG ACGCTGCCGT TGCGCAAGCG CAAGCACTAC 
ATGAACAAGG GCGACGACGG TCAGGTGGAT TCGGAGAAGG CTAGCGGAGA TGGCACCTCC 
GCCGCCGGTG GTGCCGCTTC CGTTGGCGCC GGGGATGGAC CCGGGTCCAA GGTGATGCGA 
ATGAGCTCGG TCATTCAGTT CGCCAAGGCC TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477467.1
CDS	1156..3531
Translation	MVYYSANQLLIKTEQSSQAQFCLQVPPPLTATTTSVGLGVPPSGGQQEHFELLQTPQQRQMQLQLQDQHQQEQQQFVSYQLAIQQHQKQQQQQQHESITNAAPTAAPSAQRIKTEPVGGFPASAAVVSQVRKPSASKPQFKCDQCGMTFGSKSAHTSHTKSHSKNQDLSLNGASGAGVAAPVSTAAIELNDAGLPVGIPKSPTIKPLANVAAGADPYQCNVCQKTFAVPARLIRHYRTHTGERPFECEFCHKLFSVKENLQVHRRIHTKERPYKCDVCGRAFEHSGKLHRHMRIHTGERPHKCSVCEKTFIQSGQLVIHMRTHTGEKPYKCPEPGCGKGFTCSKQLKVHSRTHTGEKPYHCDICFRDFGYNHVLKLHRVQHYGSKCYKCTICDETFKNKKEMEAHIKGHANEVPDDEAEAAAASAAASTSAGSSAGSPSLQGVSSNSESSNHSPPSSPPATKKPRQARQPRVSKTVAATLSIPTSSPLSPSSLSSTYSPSASSMASPPPTSAHYLPVQMEADALSRDSGVSSAQPAHSTYADEEPTDLSMQQVQGQLPESTVDYYQAPPSLLELQPQPAGLTINPALLEAASIARRHDDNDDQVQDEDVHAAAWQMMQLCRGHGSLPPTEQPAPSHQPQVPTLHVSDLAANYDDTHEATVLIEHFKRGDLARHGLHKGYAPVPKYESALPNPDVVRRVEAAIGLRSSTESPERSSSPESDSLMMADRNVMTLPLRKRKHYMNKGDDGQVDSEKASGDGTSAAGGAASVGAGDGPGSKVMRMSSVIQFAKAS

Target ORF information:

RefSeq Version	NM_058119.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kruppel homolog 1, transcript variant A (Kr-h1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058119.5

ORF Insert Sequence:

ATGGTTTACT ATTCCGCCAA CCAGCTGCTT ATAAAAACGG AACAATCTAG TCAGGCGCAG 
TTCTGCCTCC AGGTTCCGCC TCCACTAACA GCGACGACCA CGAGCGTGGG CCTAGGAGTT 
CCGCCATCCG GCGGCCAACA GGAGCACTTC GAGCTGCTGC AGACGCCGCA GCAGCGACAA 
ATGCAACTGC AGCTACAGGA CCAGCACCAG CAGGAGCAGC AGCAGTTTGT CAGCTACCAA 
CTGGCCATCC AGCAGCATCA GAAGCAGCAA CAACAGCAGC AACATGAAAG TATTACGAAC 
GCAGCACCCA CAGCAGCTCC ATCAGCCCAG CGGATCAAGA CAGAGCCCGT CGGCGGATTC 
CCAGCGTCGG CAGCCGTGGT GTCGCAGGTG CGCAAGCCCA GCGCCAGCAA ACCGCAATTC 
AAGTGCGACC AGTGTGGCAT GACCTTTGGC AGCAAGTCCG CCCACACCTC ACACACCAAG 
TCGCACTCGA AGAACCAAGA TCTGTCGCTT AATGGCGCCT CTGGAGCCGG CGTTGCTGCG 
CCCGTCTCAA CCGCGGCCAT CGAGCTCAAT GATGCCGGTC TGCCGGTGGG CATTCCCAAG 
AGCCCCACCA TCAAGCCGCT GGCCAACGTG GCCGCCGGAG CAGATCCCTA TCAGTGCAAT 
GTTTGCCAGA AAACATTCGC CGTACCCGCC AGGCTGATCC GCCACTACCG CACCCACACT 
GGTGAACGGC CATTCGAGTG CGAGTTCTGC CACAAGCTGT TCAGCGTGAA GGAGAACCTC 
CAGGTGCACC GGCGCATCCA CACGAAGGAG CGTCCGTACA AGTGTGACGT CTGTGGACGG 
GCATTCGAAC ACTCCGGGAA GCTGCACCGC CACATGCGCA TTCACACCGG CGAGCGGCCA 
CACAAGTGCT CCGTGTGCGA GAAGACATTC ATCCAGTCCG GCCAGCTGGT GATCCATATG 
CGCACGCACA CCGGCGAGAA GCCGTACAAG TGCCCGGAGC CGGGATGCGG CAAAGGTTTC 
ACCTGCTCCA AGCAGCTCAA GGTGCACTCG CGAACGCACA CGGGCGAGAA GCCCTACCAC 
TGTGACATCT GCTTCCGGGA CTTTGGCTAC AATCATGTGC TGAAACTGCA TCGCGTCCAG 
CACTACGGCT CCAAGTGCTA CAAGTGCACC ATCTGCGACG AGACGTTCAA GAACAAGAAG 
GAGATGGAGG CCCACATCAA GGGCCATGCC AACGAAGTAC CCGACGACGA AGCGGAGGCG 
GCGGCTGCGT CGGCAGCAGC ATCAACGTCC GCAGGCTCCT CCGCCGGATC GCCGTCCTTG 
CAAGGAGTCA GCAGCAACTC CGAGAGCAGC AACCACTCGC CGCCCAGCTC GCCACCGGCC 
ACAAAGAAAC CTCGCCAGGC TCGCCAGCCG CGCGTTTCCA AAACCGTTGC AGCCACCCTT 
TCCATCCCTA CCTCCTCGCC GCTCTCGCCC AGCTCACTCA GCTCCACATA CTCGCCATCG 
GCCAGCAGCA TGGCCTCGCC ACCACCCACA TCGGCCCACT ACCTGCCCGT CCAGATGGAG 
GCGGATGCCT TGAGCCGGGA CAGCGGCGTG TCCAGCGCCC AGCCCGCCCA CAGCACCTAT 
GCAGACGAGG AGCCCACAGA TTTGAGCATG CAACAGGTCC AGGGTCAGCT GCCAGAAAGC 
ACTGTGGACT ACTACCAGGC ACCGCCAAGT CTACTCGAAC TGCAGCCACA ACCCGCTGGT 
CTAACCATCA ATCCTGCCCT GCTTGAAGCG GCCAGCATTG CGCGTCGCCA TGACGACAAC 
GATGACCAGG TGCAGGATGA GGATGTGCAC GCAGCCGCCT GGCAAATGAT GCAGTTGTGC 
CGCGGCCACG GCTCCTTACC CCCAACGGAG CAGCCGGCGC CAAGCCACCA GCCACAGGTG 
CCCACTCTGC ACGTCAGCGA TCTAGCGGCT AACTACGATG ACACCCATGA AGCCACCGTG 
CTGATCGAGC ACTTCAAGCG TGGTGATCTC GCTCGCCACG GGCTGCACAA GGGCTATGCA 
CCAGTGCCCA AATATGAATC CGCTCTACCC AATCCGGACG TTGTGCGACG CGTGGAGGCG 
GCCATCGGCC TGCGTTCCAG CACGGAGTCG CCGGAACGTA GCTCCTCGCC GGAGAGCGAC 
TCCCTGATGA TGGCCGACCG GAACGTGATG ACGCTGCCGT TGCGCAAGCG CAAGCACTAC 
ATGAACAAGG GCGACGACGG TCAGGTGGAT TCGGAGAAGG CTAGCGGAGA TGGCACCTCC 
GCCGCCGGTG GTGCCGCTTC CGTTGGCGCC GGGGATGGAC CCGGGTCCAA GGTGATGCGA 
ATGAGCTCGG TCATTCAGTT CGCCAAGGCC TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kr-h1 ( NM_058118.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477466.1 Drosophila melanogaster kruppel homolog 1, transcript variant B (Kr-h1), mRNA.

Price & Availability↑

CloneID	OFa13878
Clone ID Related Accession (Same CDS sequence)	NM_058118.5
Accession Version	NM_058118.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kruppel homolog 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_058118.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGAAA GCAAGAATGA TACAAAATCT TGGGCACCCA AACAAATTTG GATTAAGGAT 
GTTCTCAAGA AATCGGGCAC GGAACTGCTC GATATATCCA AGAGTCCCGC AAAGGCTGTT 
GCCGTCAAAA AATCGCCGGC GAAAGATTCA GCCACAACCA AAATGGTTTA CTATTCCGCC 
AACCAGCTGC TTATAAAAAC GGAACAATCT AGTCAGGCGC AGTTCTGCCT CCAGGTTCCG 
CCTCCACTAA CAGCGACGAC CACGAGCGTG GGCCTAGGAG TTCCGCCATC CGGCGGCCAA 
CAGGAGCACT TCGAGCTGCT GCAGACGCCG CAGCAGCGAC AAATGCAACT GCAGCTACAG 
GACCAGCACC AGCAGGAGCA GCAGCAGTTT GTCAGCTACC AACTGGCCAT CCAGCAGCAT 
CAGAAGCAGC AACAACAGCA GCAACATGAA AGTATTACGA ACGCAGCACC CACAGCAGCT 
CCATCAGCCC AGCGGATCAA GACAGAGCCC GTCGGCGGAT TCCCAGCGTC GGCAGCCGTG 
GTGTCGCAGG TGCGCAAGCC CAGCGCCAGC AAACCGCAAT TCAAGTGCGA CCAGTGTGGC 
ATGACCTTTG GCAGCAAGTC CGCCCACACC TCACACACCA AGTCGCACTC GAAGAACCAA 
GATCTGTCGC TTAATGGCGC CTCTGGAGCC GGCGTTGCTG CGCCCGTCTC AACCGCGGCC 
ATCGAGCTCA ATGATGCCGG TCTGCCGGTG GGCATTCCCA AGAGCCCCAC CATCAAGCCG 
CTGGCCAACG TGGCCGCCGG AGCAGATCCC TATCAGTGCA ATGTTTGCCA GAAAACATTC 
GCCGTACCCG CCAGGCTGAT CCGCCACTAC CGCACCCACA CTGGTGAACG GCCATTCGAG 
TGCGAGTTCT GCCACAAGCT GTTCAGCGTG AAGGAGAACC TCCAGGTGCA CCGGCGCATC 
CACACGAAGG AGCGTCCGTA CAAGTGTGAC GTCTGTGGAC GGGCATTCGA ACACTCCGGG 
AAGCTGCACC GCCACATGCG CATTCACACC GGCGAGCGGC CACACAAGTG CTCCGTGTGC 
GAGAAGACAT TCATCCAGTC CGGCCAGCTG GTGATCCATA TGCGCACGCA CACCGGCGAG 
AAGCCGTACA AGTGCCCGGA GCCGGGATGC GGCAAAGGTT TCACCTGCTC CAAGCAGCTC 
AAGGTGCACT CGCGAACGCA CACGGGCGAG AAGCCCTACC ACTGTGACAT CTGCTTCCGG 
GACTTTGGCT ACAATCATGT GCTGAAACTG CATCGCGTCC AGCACTACGG CTCCAAGTGC 
TACAAGTGCA CCATCTGCGA CGAGACGTTC AAGAACAAGA AGGAGATGGA GGCCCACATC 
AAGGGCCATG CCAACGAAGT ACCCGACGAC GAAGCGGAGG CGGCGGCTGC GTCGGCAGCA 
GCATCAACGT CCGCAGGCTC CTCCGCCGGA TCGCCGTCCT TGCAAGGAGT CAGCAGCAAC 
TCCGAGAGCA GCAACCACTC GCCGCCCAGC TCGCCACCGG CCACAAAGAA ACCTCGCCAG 
GCTCGCCAGC CGCGCGTTTC CAAAACCGTT GCAGCCACCC TTTCCATCCC TACCTCCTCG 
CCGCTCTCGC CCAGCTCACT CAGCTCCACA TACTCGCCAT CGGCCAGCAG CATGGCCTCG 
CCACCACCCA CATCGGCCCA CTACCTGCCC GTCCAGATGG AGGCGGATGC CTTGAGCCGG 
GACAGCGGCG TGTCCAGCGC CCAGCCCGCC CACAGCACCT ATGCAGACGA GGAGCCCACA 
GATTTGAGCA TGCAACAGGT CCAGGGTCAG CTGCCAGAAA GCACTGTGGA CTACTACCAG 
GCACCGCCAA GTCTACTCGA ACTGCAGCCA CAACCCGCTG GTCTAACCAT CAATCCTGCC 
CTGCTTGAAG CGGCCAGCAT TGCGCGTCGC CATGACGACA ACGATGACCA GGTGCAGGAT 
GAGGATGTGC ACGCAGCCGC CTGGCAAATG ATGCAGTTGT GCCGCGGCCA CGGCTCCTTA 
CCCCCAACGG AGCAGCCGGC GCCAAGCCAC CAGCCACAGG TGCCCACTCT GCACGTCAGC 
GATCTAGCGG CTAACTACGA TGACACCCAT GAAGCCACCG TGCTGATCGA GCACTTCAAG 
CGTGGTGATC TCGCTCGCCA CGGGCTGCAC AAGGGCTATG CACCAGTGCC CAAATATGAA 
TCCGCTCTAC CCAATCCGGA CGTTGTGCGA CGCGTGGAGG CGGCCATCGG CCTGCGTTCC 
AGCACGGAGT CGCCGGAACG TAGCTCCTCG CCGGAGAGCG ACTCCCTGAT GATGGCCGAC 
CGGAACGTGA TGACGCTGCC GTTGCGCAAG CGCAAGCACT ACATGAACAA GGGCGACGAC 
GGTCAGGTGG ATTCGGAGAA GGCTAGCGGA GATGGCACCT CCGCCGCCGG TGGTGCCGCT 
TCCGTTGGCG CCGGGGATGG ACCCGGGTCC AAGGTGATGC GAATGAGCTC GGTCATTCAG 
TTCGCCAAGG CCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477466.1
CDS	689..3226
Translation	MTESKNDTKSWAPKQIWIKDVLKKSGTELLDISKSPAKAVAVKKSPAKDSATTKMVYYSANQLLIKTEQSSQAQFCLQVPPPLTATTTSVGLGVPPSGGQQEHFELLQTPQQRQMQLQLQDQHQQEQQQFVSYQLAIQQHQKQQQQQQHESITNAAPTAAPSAQRIKTEPVGGFPASAAVVSQVRKPSASKPQFKCDQCGMTFGSKSAHTSHTKSHSKNQDLSLNGASGAGVAAPVSTAAIELNDAGLPVGIPKSPTIKPLANVAAGADPYQCNVCQKTFAVPARLIRHYRTHTGERPFECEFCHKLFSVKENLQVHRRIHTKERPYKCDVCGRAFEHSGKLHRHMRIHTGERPHKCSVCEKTFIQSGQLVIHMRTHTGEKPYKCPEPGCGKGFTCSKQLKVHSRTHTGEKPYHCDICFRDFGYNHVLKLHRVQHYGSKCYKCTICDETFKNKKEMEAHIKGHANEVPDDEAEAAAASAAASTSAGSSAGSPSLQGVSSNSESSNHSPPSSPPATKKPRQARQPRVSKTVAATLSIPTSSPLSPSSLSSTYSPSASSMASPPPTSAHYLPVQMEADALSRDSGVSSAQPAHSTYADEEPTDLSMQQVQGQLPESTVDYYQAPPSLLELQPQPAGLTINPALLEAASIARRHDDNDDQVQDEDVHAAAWQMMQLCRGHGSLPPTEQPAPSHQPQVPTLHVSDLAANYDDTHEATVLIEHFKRGDLARHGLHKGYAPVPKYESALPNPDVVRRVEAAIGLRSSTESPERSSSPESDSLMMADRNVMTLPLRKRKHYMNKGDDGQVDSEKASGDGTSAAGGAASVGAGDGPGSKVMRMSSVIQFAKAS

Target ORF information:

RefSeq Version	NM_058118.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kruppel homolog 1, transcript variant B (Kr-h1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058118.5

ORF Insert Sequence:

ATGACTGAAA GCAAGAATGA TACAAAATCT TGGGCACCCA AACAAATTTG GATTAAGGAT 
GTTCTCAAGA AATCGGGCAC GGAACTGCTC GATATATCCA AGAGTCCCGC AAAGGCTGTT 
GCCGTCAAAA AATCGCCGGC GAAAGATTCA GCCACAACCA AAATGGTTTA CTATTCCGCC 
AACCAGCTGC TTATAAAAAC GGAACAATCT AGTCAGGCGC AGTTCTGCCT CCAGGTTCCG 
CCTCCACTAA CAGCGACGAC CACGAGCGTG GGCCTAGGAG TTCCGCCATC CGGCGGCCAA 
CAGGAGCACT TCGAGCTGCT GCAGACGCCG CAGCAGCGAC AAATGCAACT GCAGCTACAG 
GACCAGCACC AGCAGGAGCA GCAGCAGTTT GTCAGCTACC AACTGGCCAT CCAGCAGCAT 
CAGAAGCAGC AACAACAGCA GCAACATGAA AGTATTACGA ACGCAGCACC CACAGCAGCT 
CCATCAGCCC AGCGGATCAA GACAGAGCCC GTCGGCGGAT TCCCAGCGTC GGCAGCCGTG 
GTGTCGCAGG TGCGCAAGCC CAGCGCCAGC AAACCGCAAT TCAAGTGCGA CCAGTGTGGC 
ATGACCTTTG GCAGCAAGTC CGCCCACACC TCACACACCA AGTCGCACTC GAAGAACCAA 
GATCTGTCGC TTAATGGCGC CTCTGGAGCC GGCGTTGCTG CGCCCGTCTC AACCGCGGCC 
ATCGAGCTCA ATGATGCCGG TCTGCCGGTG GGCATTCCCA AGAGCCCCAC CATCAAGCCG 
CTGGCCAACG TGGCCGCCGG AGCAGATCCC TATCAGTGCA ATGTTTGCCA GAAAACATTC 
GCCGTACCCG CCAGGCTGAT CCGCCACTAC CGCACCCACA CTGGTGAACG GCCATTCGAG 
TGCGAGTTCT GCCACAAGCT GTTCAGCGTG AAGGAGAACC TCCAGGTGCA CCGGCGCATC 
CACACGAAGG AGCGTCCGTA CAAGTGTGAC GTCTGTGGAC GGGCATTCGA ACACTCCGGG 
AAGCTGCACC GCCACATGCG CATTCACACC GGCGAGCGGC CACACAAGTG CTCCGTGTGC 
GAGAAGACAT TCATCCAGTC CGGCCAGCTG GTGATCCATA TGCGCACGCA CACCGGCGAG 
AAGCCGTACA AGTGCCCGGA GCCGGGATGC GGCAAAGGTT TCACCTGCTC CAAGCAGCTC 
AAGGTGCACT CGCGAACGCA CACGGGCGAG AAGCCCTACC ACTGTGACAT CTGCTTCCGG 
GACTTTGGCT ACAATCATGT GCTGAAACTG CATCGCGTCC AGCACTACGG CTCCAAGTGC 
TACAAGTGCA CCATCTGCGA CGAGACGTTC AAGAACAAGA AGGAGATGGA GGCCCACATC 
AAGGGCCATG CCAACGAAGT ACCCGACGAC GAAGCGGAGG CGGCGGCTGC GTCGGCAGCA 
GCATCAACGT CCGCAGGCTC CTCCGCCGGA TCGCCGTCCT TGCAAGGAGT CAGCAGCAAC 
TCCGAGAGCA GCAACCACTC GCCGCCCAGC TCGCCACCGG CCACAAAGAA ACCTCGCCAG 
GCTCGCCAGC CGCGCGTTTC CAAAACCGTT GCAGCCACCC TTTCCATCCC TACCTCCTCG 
CCGCTCTCGC CCAGCTCACT CAGCTCCACA TACTCGCCAT CGGCCAGCAG CATGGCCTCG 
CCACCACCCA CATCGGCCCA CTACCTGCCC GTCCAGATGG AGGCGGATGC CTTGAGCCGG 
GACAGCGGCG TGTCCAGCGC CCAGCCCGCC CACAGCACCT ATGCAGACGA GGAGCCCACA 
GATTTGAGCA TGCAACAGGT CCAGGGTCAG CTGCCAGAAA GCACTGTGGA CTACTACCAG 
GCACCGCCAA GTCTACTCGA ACTGCAGCCA CAACCCGCTG GTCTAACCAT CAATCCTGCC 
CTGCTTGAAG CGGCCAGCAT TGCGCGTCGC CATGACGACA ACGATGACCA GGTGCAGGAT 
GAGGATGTGC ACGCAGCCGC CTGGCAAATG ATGCAGTTGT GCCGCGGCCA CGGCTCCTTA 
CCCCCAACGG AGCAGCCGGC GCCAAGCCAC CAGCCACAGG TGCCCACTCT GCACGTCAGC 
GATCTAGCGG CTAACTACGA TGACACCCAT GAAGCCACCG TGCTGATCGA GCACTTCAAG 
CGTGGTGATC TCGCTCGCCA CGGGCTGCAC AAGGGCTATG CACCAGTGCC CAAATATGAA 
TCCGCTCTAC CCAATCCGGA CGTTGTGCGA CGCGTGGAGG CGGCCATCGG CCTGCGTTCC 
AGCACGGAGT CGCCGGAACG TAGCTCCTCG CCGGAGAGCG ACTCCCTGAT GATGGCCGAC 
CGGAACGTGA TGACGCTGCC GTTGCGCAAG CGCAAGCACT ACATGAACAA GGGCGACGAC 
GGTCAGGTGG ATTCGGAGAA GGCTAGCGGA GATGGCACCT CCGCCGCCGG TGGTGCCGCT 
TCCGTTGGCG CCGGGGATGG ACCCGGGTCC AAGGTGATGC GAATGAGCTC GGTCATTCAG 
TTCGCCAAGG CCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Kr-h1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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