eIF4A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eIF4A
Entrez Gene ID	33835
Full Name	Eukaryotic initiation factor 4a
Synonyms	2439,9075,CG9075,Dm-eIF4A,DmRH2,Dmel\CG9075,EIF-4A,EIF-4a,EIF4A,EiF4A,Eif-4a,Eif4a,L(2L)162,Su(dpp)YE9,anon-26Aa,anon26A,deIF4A,eIF 4A,eIF-4A,eIF-4a-I,eIF4A,eIF4A-I,eIF4AI,eIF4a,eiF4A,eif-4a,eif4A,eif4a,l(2)02439,l(2)162,l(2)26Ac,l(2)gdh-4,l(2)k01501,l(2L)162,pecan
General protein information	Preferred Names Eukaryotic initiation factor 4a Names CG9075 gene product from transcript CG9075-RE CG9075-PA CG9075-PB CG9075-PC CG9075-PD CG9075-PE CG9075-PF eIF-4a-PA eIF-4a-PB eIF-4a-PC eIF-4a-PD eIF-4a-PE eIF-4a-PF eukaryotic initiation factor 4A eukaryotic initiation factor 4a eukaryotic translation initiation factor 4A translation initiation factor 4A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26B2-26B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_164669.3	NP_723138.1	eukaryotic translation initiation factor 4A, isoform B
NM_001273188.1	NP_001260117.1	eukaryotic translation initiation factor 4A, isoform F
NM_164668.3	NP_723137.1	eukaryotic translation initiation factor 4A, isoform A
NM_057247.5	NP_476595.1	eukaryotic translation initiation factor 4A, isoform C
NM_164670.4	NP_723139.1	eukaryotic translation initiation factor 4A, isoform D
NM_001258978.3	NP_001245907.1	eukaryotic translation initiation factor 4A, isoform E

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os06g0701100	NP_001058481.1
Pan troglodytes (chimpanzee)	EIF4A2	NP_001233447.1
Canis lupus familiaris (dog)	EIF4A2	XP_860499.2
Bos taurus (cattle)	EIF4A2	NP_001029216.1
Homo sapiens (human)	EIF4A2	NP_001958.2
	EIF4A2	XP_001102961.2
Rattus norvegicus (Norway rat)	Eif4a2	NP_001008336.1
Drosophila melanogaster (fruit fly)	eIF-4a	NP_001245907.1
Arabidopsis thaliana (thale cress)	EIF4A-2	NP_175829.1
Arabidopsis thaliana (thale cress)	EIF4A1	NP_566469.1
Oryza sativa (rice)	Os02g0146600	NP_001045878.1
Mus musculus (house mouse)	Eif4a2	NP_038534.2
Gallus gallus (chicken)	EIF4A2	NP_989880.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009863	XP_318978.3
Caenorhabditis elegans (roundworm)	inf-1	NP_001022623.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our data identify the eIF4F complex as an important upstream regulator of TORC1, which acts via TSC2 to inactivate TORC1 upon withdrawal of amino acids
Title: eIF4A inactivates TORC1 in response to amino acid?starvation.

eIF4A controls germline stem cell self-renewal by directly inhibiting BAM function in the Drosophila ovary.
Title: eIF4A controls germline stem cell self-renewal by directly inhibiting BAM function in the Drosophila ovary.

The results suggest that the eIF4A gene is under the control of the DREF pathway and DREF is therefore involved in the regulation of protein synthesis.
Title: Identification of the Drosophila eIF4A gene as a target of the DREF transcription factor.

eIF4A has a novel function as a specific inhibitor of Dpp signalling that mediates the degradation of SMAD homologues.
Title: A novel function of Drosophila eIF4A as a negative regulator of Dpp/BMP signalling that mediates SMAD degradation.

dIF4A is a maternal-effect suppressor of decapentalplegic.
Title: A genetic screen for maternal-effect suppressors of decapentaplegic identifies the eukaryotic translation initiation factor 4A in Drosophila.

The following eIF4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eIF4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13851	NM_001258978.3 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13851	NM_057247.5 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13851	NM_164669.3 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13851	NM_001273188.1 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13851	NM_164670.4 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13851	NM_164668.3 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eIF4A ( NM_164669.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723138.1 Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13851
Clone ID Related Accession (Same CDS sequence)	NM_164669.3 , NM_001273188.1 , NM_164668.3 , NM_057247.5 , NM_164670.4 , NM_001258978.3
Accession Version	NM_164669.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164669.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGACC GAAATGAGAT ACCTCAGGAT GGCCCCGCCA GCATGGAACC CGAGGGCGTC 
ATCGAGTCCA CCTGGCACGA GGTGTACGAC AACTTCGATG ACATGAACTT GCGCGAGGAG 
TTGCTGCGCG GCATCTACGG TTATGGTTTC GAGAAGCCGT CGGCCATCCA GCAGCGCGCC 
ATCATTCCCT GTGTGAGGGG TCGCGATGTC ATTGCCCAGG CCCAGTCGGG AACTGGCAAG 
ACTGCCACCT TCTCGATTGC TATCCTTCAG CAAATCGACA CGAGCATTCG CGAGTGCCAG 
GCGCTGATCC TGGCCCCCAC TCGCGAGTTG GCCACGCAGA TCCAGCGCGT GGTGATGGCG 
CTCGGCGAGT ACATGAAGGT GCACTCGCAC GCCTGCATTG GCGGCACTAA CGTGCGCGAA 
GACGCCCGCA TCTTGGAATC CGGTTGCCAT GTGGTGGTGG GCACTCCTGG CCGCGTCTAC 
GACATGATCA ACCGCAAGGT GCTGCGCACC CAGTACATCA AGCTGTTCGT GCTGGATGAG 
GCCGATGAGA TGTTGTCCCG CGGTTTCAAG GATCAGATCC AGGATGTCTT CAAGATGCTG 
CCCCCAGATG TGCAGGTCAT CCTGCTGTCC GCCACCATGC CGCCGGATGT GCTCGAAGTG 
AGCCGTTGCT TCATGCGTGA TCCCGTCAGC ATCCTGGTTA AGAAGGAGGA ACTGACCCTT 
GAGGGTATCA AGCAGTTTTA CGTCAACGTG AAGCAGGAGA ACTGGAAACT GGGCACCCTT 
TGCGATCTGT ACGATACGCT GTCCATCACC CAGTCGGTAA TCTTCTGCAA CACCCGTCGC 
AAGGTGGACC AACTGACCCA GGAGATGTCT ATCCACAACT TCACCGTCTC GGCCATGCAC 
GGCGACATGG AGCAGCGTGA TCGCGAGGTC ATCATGAAAC AATTCCGTTC GGGCTCGTCT 
CGTGTTCTGA TTACCACTGA TTTACTGGCG CGCGGTATTG ATGTGCAGCA GGTGTCGTTG 
GTCATCAACT ATGATCTGCC CTCGAACCGC GAGAACTACA TTCATAGAAT TGGTCGCGGT 
GGTCGTTTCG GTCGCAAGGG TGTTGCGATC AACTTTATTA CAGATGATGA TCGACGAATC 
CTTAAGGATA TTGAACAGTT CTACCACACA ACAATTGAGG AAATGCCTGC TAATATTGCC 
GATTTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723138.1
CDS	227..1438
Translation	MDDRNEIPQDGPASMEPEGVIESTWHEVYDNFDDMNLREELLRGIYGYGFEKPSAIQQRAIIPCVRGRDVIAQAQSGTGKTATFSIAILQQIDTSIRECQALILAPTRELATQIQRVVMALGEYMKVHSHACIGGTNVREDARILESGCHVVVGTPGRVYDMINRKVLRTQYIKLFVLDEADEMLSRGFKDQIQDVFKMLPPDVQVILLSATMPPDVLEVSRCFMRDPVSILVKKEELTLEGIKQFYVNVKQENWKLGTLCDLYDTLSITQSVIFCNTRRKVDQLTQEMSIHNFTVSAMHGDMEQRDREVIMKQFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPSNRENYIHRIGRGGRFGRKGVAINFITDDDRRILKDIEQFYHTTIEEMPANIADLI

Target ORF information:

RefSeq Version	NM_164669.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164669.3

ORF Insert Sequence:

ATGGATGACC GAAATGAGAT ACCTCAGGAT GGCCCCGCCA GCATGGAACC CGAGGGCGTC 
ATCGAGTCCA CCTGGCACGA GGTGTACGAC AACTTCGATG ACATGAACTT GCGCGAGGAG 
TTGCTGCGCG GCATCTACGG TTATGGTTTC GAGAAGCCGT CGGCCATCCA GCAGCGCGCC 
ATCATTCCCT GTGTGAGGGG TCGCGATGTC ATTGCCCAGG CCCAGTCGGG AACTGGCAAG 
ACTGCCACCT TCTCGATTGC TATCCTTCAG CAAATCGACA CGAGCATTCG CGAGTGCCAG 
GCGCTGATCC TGGCCCCCAC TCGCGAGTTG GCCACGCAGA TCCAGCGCGT GGTGATGGCG 
CTCGGCGAGT ACATGAAGGT GCACTCGCAC GCCTGCATTG GCGGCACTAA CGTGCGCGAA 
GACGCCCGCA TCTTGGAATC CGGTTGCCAT GTGGTGGTGG GCACTCCTGG CCGCGTCTAC 
GACATGATCA ACCGCAAGGT GCTGCGCACC CAGTACATCA AGCTGTTCGT GCTGGATGAG 
GCCGATGAGA TGTTGTCCCG CGGTTTCAAG GATCAGATCC AGGATGTCTT CAAGATGCTG 
CCCCCAGATG TGCAGGTCAT CCTGCTGTCC GCCACCATGC CGCCGGATGT GCTCGAAGTG 
AGCCGTTGCT TCATGCGTGA TCCCGTCAGC ATCCTGGTTA AGAAGGAGGA ACTGACCCTT 
GAGGGTATCA AGCAGTTTTA CGTCAACGTG AAGCAGGAGA ACTGGAAACT GGGCACCCTT 
TGCGATCTGT ACGATACGCT GTCCATCACC CAGTCGGTAA TCTTCTGCAA CACCCGTCGC 
AAGGTGGACC AACTGACCCA GGAGATGTCT ATCCACAACT TCACCGTCTC GGCCATGCAC 
GGCGACATGG AGCAGCGTGA TCGCGAGGTC ATCATGAAAC AATTCCGTTC GGGCTCGTCT 
CGTGTTCTGA TTACCACTGA TTTACTGGCG CGCGGTATTG ATGTGCAGCA GGTGTCGTTG 
GTCATCAACT ATGATCTGCC CTCGAACCGC GAGAACTACA TTCATAGAAT TGGTCGCGGT 
GGTCGTTTCG GTCGCAAGGG TGTTGCGATC AACTTTATTA CAGATGATGA TCGACGAATC 
CTTAAGGATA TTGAACAGTT CTACCACACA ACAATTGAGG AAATGCCTGC TAATATTGCC 
GATTTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF4A ( NM_001273188.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260117.1 Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa13851
Clone ID Related Accession (Same CDS sequence)	NM_164669.3 , NM_001273188.1 , NM_164668.3 , NM_057247.5 , NM_164670.4 , NM_001258978.3
Accession Version	NM_001273188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4A, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGACC GAAATGAGAT ACCTCAGGAT GGCCCCGCCA GCATGGAACC CGAGGGCGTC 
ATCGAGTCCA CCTGGCACGA GGTGTACGAC AACTTCGATG ACATGAACTT GCGCGAGGAG 
TTGCTGCGCG GCATCTACGG TTATGGTTTC GAGAAGCCGT CGGCCATCCA GCAGCGCGCC 
ATCATTCCCT GTGTGAGGGG TCGCGATGTC ATTGCCCAGG CCCAGTCGGG AACTGGCAAG 
ACTGCCACCT TCTCGATTGC TATCCTTCAG CAAATCGACA CGAGCATTCG CGAGTGCCAG 
GCGCTGATCC TGGCCCCCAC TCGCGAGTTG GCCACGCAGA TCCAGCGCGT GGTGATGGCG 
CTCGGCGAGT ACATGAAGGT GCACTCGCAC GCCTGCATTG GCGGCACTAA CGTGCGCGAA 
GACGCCCGCA TCTTGGAATC CGGTTGCCAT GTGGTGGTGG GCACTCCTGG CCGCGTCTAC 
GACATGATCA ACCGCAAGGT GCTGCGCACC CAGTACATCA AGCTGTTCGT GCTGGATGAG 
GCCGATGAGA TGTTGTCCCG CGGTTTCAAG GATCAGATCC AGGATGTCTT CAAGATGCTG 
CCCCCAGATG TGCAGGTCAT CCTGCTGTCC GCCACCATGC CGCCGGATGT GCTCGAAGTG 
AGCCGTTGCT TCATGCGTGA TCCCGTCAGC ATCCTGGTTA AGAAGGAGGA ACTGACCCTT 
GAGGGTATCA AGCAGTTTTA CGTCAACGTG AAGCAGGAGA ACTGGAAACT GGGCACCCTT 
TGCGATCTGT ACGATACGCT GTCCATCACC CAGTCGGTAA TCTTCTGCAA CACCCGTCGC 
AAGGTGGACC AACTGACCCA GGAGATGTCT ATCCACAACT TCACCGTCTC GGCCATGCAC 
GGCGACATGG AGCAGCGTGA TCGCGAGGTC ATCATGAAAC AATTCCGTTC GGGCTCGTCT 
CGTGTTCTGA TTACCACTGA TTTACTGGCG CGCGGTATTG ATGTGCAGCA GGTGTCGTTG 
GTCATCAACT ATGATCTGCC CTCGAACCGC GAGAACTACA TTCATAGAAT TGGTCGCGGT 
GGTCGTTTCG GTCGCAAGGG TGTTGCGATC AACTTTATTA CAGATGATGA TCGACGAATC 
CTTAAGGATA TTGAACAGTT CTACCACACA ACAATTGAGG AAATGCCTGC TAATATTGCC 
GATTTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260117.1
CDS	227..1438
Translation	MDDRNEIPQDGPASMEPEGVIESTWHEVYDNFDDMNLREELLRGIYGYGFEKPSAIQQRAIIPCVRGRDVIAQAQSGTGKTATFSIAILQQIDTSIRECQALILAPTRELATQIQRVVMALGEYMKVHSHACIGGTNVREDARILESGCHVVVGTPGRVYDMINRKVLRTQYIKLFVLDEADEMLSRGFKDQIQDVFKMLPPDVQVILLSATMPPDVLEVSRCFMRDPVSILVKKEELTLEGIKQFYVNVKQENWKLGTLCDLYDTLSITQSVIFCNTRRKVDQLTQEMSIHNFTVSAMHGDMEQRDREVIMKQFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPSNRENYIHRIGRGGRFGRKGVAINFITDDDRRILKDIEQFYHTTIEEMPANIADLI

Target ORF information:

RefSeq Version	NM_001273188.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273188.1

ORF Insert Sequence:

ATGGATGACC GAAATGAGAT ACCTCAGGAT GGCCCCGCCA GCATGGAACC CGAGGGCGTC 
ATCGAGTCCA CCTGGCACGA GGTGTACGAC AACTTCGATG ACATGAACTT GCGCGAGGAG 
TTGCTGCGCG GCATCTACGG TTATGGTTTC GAGAAGCCGT CGGCCATCCA GCAGCGCGCC 
ATCATTCCCT GTGTGAGGGG TCGCGATGTC ATTGCCCAGG CCCAGTCGGG AACTGGCAAG 
ACTGCCACCT TCTCGATTGC TATCCTTCAG CAAATCGACA CGAGCATTCG CGAGTGCCAG 
GCGCTGATCC TGGCCCCCAC TCGCGAGTTG GCCACGCAGA TCCAGCGCGT GGTGATGGCG 
CTCGGCGAGT ACATGAAGGT GCACTCGCAC GCCTGCATTG GCGGCACTAA CGTGCGCGAA 
GACGCCCGCA TCTTGGAATC CGGTTGCCAT GTGGTGGTGG GCACTCCTGG CCGCGTCTAC 
GACATGATCA ACCGCAAGGT GCTGCGCACC CAGTACATCA AGCTGTTCGT GCTGGATGAG 
GCCGATGAGA TGTTGTCCCG CGGTTTCAAG GATCAGATCC AGGATGTCTT CAAGATGCTG 
CCCCCAGATG TGCAGGTCAT CCTGCTGTCC GCCACCATGC CGCCGGATGT GCTCGAAGTG 
AGCCGTTGCT TCATGCGTGA TCCCGTCAGC ATCCTGGTTA AGAAGGAGGA ACTGACCCTT 
GAGGGTATCA AGCAGTTTTA CGTCAACGTG AAGCAGGAGA ACTGGAAACT GGGCACCCTT 
TGCGATCTGT ACGATACGCT GTCCATCACC CAGTCGGTAA TCTTCTGCAA CACCCGTCGC 
AAGGTGGACC AACTGACCCA GGAGATGTCT ATCCACAACT TCACCGTCTC GGCCATGCAC 
GGCGACATGG AGCAGCGTGA TCGCGAGGTC ATCATGAAAC AATTCCGTTC GGGCTCGTCT 
CGTGTTCTGA TTACCACTGA TTTACTGGCG CGCGGTATTG ATGTGCAGCA GGTGTCGTTG 
GTCATCAACT ATGATCTGCC CTCGAACCGC GAGAACTACA TTCATAGAAT TGGTCGCGGT 
GGTCGTTTCG GTCGCAAGGG TGTTGCGATC AACTTTATTA CAGATGATGA TCGACGAATC 
CTTAAGGATA TTGAACAGTT CTACCACACA ACAATTGAGG AAATGCCTGC TAATATTGCC 
GATTTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF4A ( NM_164668.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723137.1 Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13851
Clone ID Related Accession (Same CDS sequence)	NM_164669.3 , NM_001273188.1 , NM_164668.3 , NM_057247.5 , NM_164670.4 , NM_001258978.3
Accession Version	NM_164668.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164668.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGACC GAAATGAGAT ACCTCAGGAT GGCCCCGCCA GCATGGAACC CGAGGGCGTC 
ATCGAGTCCA CCTGGCACGA GGTGTACGAC AACTTCGATG ACATGAACTT GCGCGAGGAG 
TTGCTGCGCG GCATCTACGG TTATGGTTTC GAGAAGCCGT CGGCCATCCA GCAGCGCGCC 
ATCATTCCCT GTGTGAGGGG TCGCGATGTC ATTGCCCAGG CCCAGTCGGG AACTGGCAAG 
ACTGCCACCT TCTCGATTGC TATCCTTCAG CAAATCGACA CGAGCATTCG CGAGTGCCAG 
GCGCTGATCC TGGCCCCCAC TCGCGAGTTG GCCACGCAGA TCCAGCGCGT GGTGATGGCG 
CTCGGCGAGT ACATGAAGGT GCACTCGCAC GCCTGCATTG GCGGCACTAA CGTGCGCGAA 
GACGCCCGCA TCTTGGAATC CGGTTGCCAT GTGGTGGTGG GCACTCCTGG CCGCGTCTAC 
GACATGATCA ACCGCAAGGT GCTGCGCACC CAGTACATCA AGCTGTTCGT GCTGGATGAG 
GCCGATGAGA TGTTGTCCCG CGGTTTCAAG GATCAGATCC AGGATGTCTT CAAGATGCTG 
CCCCCAGATG TGCAGGTCAT CCTGCTGTCC GCCACCATGC CGCCGGATGT GCTCGAAGTG 
AGCCGTTGCT TCATGCGTGA TCCCGTCAGC ATCCTGGTTA AGAAGGAGGA ACTGACCCTT 
GAGGGTATCA AGCAGTTTTA CGTCAACGTG AAGCAGGAGA ACTGGAAACT GGGCACCCTT 
TGCGATCTGT ACGATACGCT GTCCATCACC CAGTCGGTAA TCTTCTGCAA CACCCGTCGC 
AAGGTGGACC AACTGACCCA GGAGATGTCT ATCCACAACT TCACCGTCTC GGCCATGCAC 
GGCGACATGG AGCAGCGTGA TCGCGAGGTC ATCATGAAAC AATTCCGTTC GGGCTCGTCT 
CGTGTTCTGA TTACCACTGA TTTACTGGCG CGCGGTATTG ATGTGCAGCA GGTGTCGTTG 
GTCATCAACT ATGATCTGCC CTCGAACCGC GAGAACTACA TTCATAGAAT TGGTCGCGGT 
GGTCGTTTCG GTCGCAAGGG TGTTGCGATC AACTTTATTA CAGATGATGA TCGACGAATC 
CTTAAGGATA TTGAACAGTT CTACCACACA ACAATTGAGG AAATGCCTGC TAATATTGCC 
GATTTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723137.1
CDS	71..1282
Translation	MDDRNEIPQDGPASMEPEGVIESTWHEVYDNFDDMNLREELLRGIYGYGFEKPSAIQQRAIIPCVRGRDVIAQAQSGTGKTATFSIAILQQIDTSIRECQALILAPTRELATQIQRVVMALGEYMKVHSHACIGGTNVREDARILESGCHVVVGTPGRVYDMINRKVLRTQYIKLFVLDEADEMLSRGFKDQIQDVFKMLPPDVQVILLSATMPPDVLEVSRCFMRDPVSILVKKEELTLEGIKQFYVNVKQENWKLGTLCDLYDTLSITQSVIFCNTRRKVDQLTQEMSIHNFTVSAMHGDMEQRDREVIMKQFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPSNRENYIHRIGRGGRFGRKGVAINFITDDDRRILKDIEQFYHTTIEEMPANIADLI

Target ORF information:

RefSeq Version	NM_164668.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164668.3

ORF Insert Sequence:

ATGGATGACC GAAATGAGAT ACCTCAGGAT GGCCCCGCCA GCATGGAACC CGAGGGCGTC 
ATCGAGTCCA CCTGGCACGA GGTGTACGAC AACTTCGATG ACATGAACTT GCGCGAGGAG 
TTGCTGCGCG GCATCTACGG TTATGGTTTC GAGAAGCCGT CGGCCATCCA GCAGCGCGCC 
ATCATTCCCT GTGTGAGGGG TCGCGATGTC ATTGCCCAGG CCCAGTCGGG AACTGGCAAG 
ACTGCCACCT TCTCGATTGC TATCCTTCAG CAAATCGACA CGAGCATTCG CGAGTGCCAG 
GCGCTGATCC TGGCCCCCAC TCGCGAGTTG GCCACGCAGA TCCAGCGCGT GGTGATGGCG 
CTCGGCGAGT ACATGAAGGT GCACTCGCAC GCCTGCATTG GCGGCACTAA CGTGCGCGAA 
GACGCCCGCA TCTTGGAATC CGGTTGCCAT GTGGTGGTGG GCACTCCTGG CCGCGTCTAC 
GACATGATCA ACCGCAAGGT GCTGCGCACC CAGTACATCA AGCTGTTCGT GCTGGATGAG 
GCCGATGAGA TGTTGTCCCG CGGTTTCAAG GATCAGATCC AGGATGTCTT CAAGATGCTG 
CCCCCAGATG TGCAGGTCAT CCTGCTGTCC GCCACCATGC CGCCGGATGT GCTCGAAGTG 
AGCCGTTGCT TCATGCGTGA TCCCGTCAGC ATCCTGGTTA AGAAGGAGGA ACTGACCCTT 
GAGGGTATCA AGCAGTTTTA CGTCAACGTG AAGCAGGAGA ACTGGAAACT GGGCACCCTT 
TGCGATCTGT ACGATACGCT GTCCATCACC CAGTCGGTAA TCTTCTGCAA CACCCGTCGC 
AAGGTGGACC AACTGACCCA GGAGATGTCT ATCCACAACT TCACCGTCTC GGCCATGCAC 
GGCGACATGG AGCAGCGTGA TCGCGAGGTC ATCATGAAAC AATTCCGTTC GGGCTCGTCT 
CGTGTTCTGA TTACCACTGA TTTACTGGCG CGCGGTATTG ATGTGCAGCA GGTGTCGTTG 
GTCATCAACT ATGATCTGCC CTCGAACCGC GAGAACTACA TTCATAGAAT TGGTCGCGGT 
GGTCGTTTCG GTCGCAAGGG TGTTGCGATC AACTTTATTA CAGATGATGA TCGACGAATC 
CTTAAGGATA TTGAACAGTT CTACCACACA ACAATTGAGG AAATGCCTGC TAATATTGCC 
GATTTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF4A ( NM_057247.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476595.1 Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant C, mRNA.

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CloneID	OFa13851
Clone ID Related Accession (Same CDS sequence)	NM_164669.3 , NM_001273188.1 , NM_164668.3 , NM_057247.5 , NM_164670.4 , NM_001258978.3
Accession Version	NM_057247.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4A, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057247.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGACC GAAATGAGAT ACCTCAGGAT GGCCCCGCCA GCATGGAACC CGAGGGCGTC 
ATCGAGTCCA CCTGGCACGA GGTGTACGAC AACTTCGATG ACATGAACTT GCGCGAGGAG 
TTGCTGCGCG GCATCTACGG TTATGGTTTC GAGAAGCCGT CGGCCATCCA GCAGCGCGCC 
ATCATTCCCT GTGTGAGGGG TCGCGATGTC ATTGCCCAGG CCCAGTCGGG AACTGGCAAG 
ACTGCCACCT TCTCGATTGC TATCCTTCAG CAAATCGACA CGAGCATTCG CGAGTGCCAG 
GCGCTGATCC TGGCCCCCAC TCGCGAGTTG GCCACGCAGA TCCAGCGCGT GGTGATGGCG 
CTCGGCGAGT ACATGAAGGT GCACTCGCAC GCCTGCATTG GCGGCACTAA CGTGCGCGAA 
GACGCCCGCA TCTTGGAATC CGGTTGCCAT GTGGTGGTGG GCACTCCTGG CCGCGTCTAC 
GACATGATCA ACCGCAAGGT GCTGCGCACC CAGTACATCA AGCTGTTCGT GCTGGATGAG 
GCCGATGAGA TGTTGTCCCG CGGTTTCAAG GATCAGATCC AGGATGTCTT CAAGATGCTG 
CCCCCAGATG TGCAGGTCAT CCTGCTGTCC GCCACCATGC CGCCGGATGT GCTCGAAGTG 
AGCCGTTGCT TCATGCGTGA TCCCGTCAGC ATCCTGGTTA AGAAGGAGGA ACTGACCCTT 
GAGGGTATCA AGCAGTTTTA CGTCAACGTG AAGCAGGAGA ACTGGAAACT GGGCACCCTT 
TGCGATCTGT ACGATACGCT GTCCATCACC CAGTCGGTAA TCTTCTGCAA CACCCGTCGC 
AAGGTGGACC AACTGACCCA GGAGATGTCT ATCCACAACT TCACCGTCTC GGCCATGCAC 
GGCGACATGG AGCAGCGTGA TCGCGAGGTC ATCATGAAAC AATTCCGTTC GGGCTCGTCT 
CGTGTTCTGA TTACCACTGA TTTACTGGCG CGCGGTATTG ATGTGCAGCA GGTGTCGTTG 
GTCATCAACT ATGATCTGCC CTCGAACCGC GAGAACTACA TTCATAGAAT TGGTCGCGGT 
GGTCGTTTCG GTCGCAAGGG TGTTGCGATC AACTTTATTA CAGATGATGA TCGACGAATC 
CTTAAGGATA TTGAACAGTT CTACCACACA ACAATTGAGG AAATGCCTGC TAATATTGCC 
GATTTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476595.1
CDS	71..1282
Translation	MDDRNEIPQDGPASMEPEGVIESTWHEVYDNFDDMNLREELLRGIYGYGFEKPSAIQQRAIIPCVRGRDVIAQAQSGTGKTATFSIAILQQIDTSIRECQALILAPTRELATQIQRVVMALGEYMKVHSHACIGGTNVREDARILESGCHVVVGTPGRVYDMINRKVLRTQYIKLFVLDEADEMLSRGFKDQIQDVFKMLPPDVQVILLSATMPPDVLEVSRCFMRDPVSILVKKEELTLEGIKQFYVNVKQENWKLGTLCDLYDTLSITQSVIFCNTRRKVDQLTQEMSIHNFTVSAMHGDMEQRDREVIMKQFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPSNRENYIHRIGRGGRFGRKGVAINFITDDDRRILKDIEQFYHTTIEEMPANIADLI

Target ORF information:

RefSeq Version	NM_057247.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057247.5

ORF Insert Sequence:

ATGGATGACC GAAATGAGAT ACCTCAGGAT GGCCCCGCCA GCATGGAACC CGAGGGCGTC 
ATCGAGTCCA CCTGGCACGA GGTGTACGAC AACTTCGATG ACATGAACTT GCGCGAGGAG 
TTGCTGCGCG GCATCTACGG TTATGGTTTC GAGAAGCCGT CGGCCATCCA GCAGCGCGCC 
ATCATTCCCT GTGTGAGGGG TCGCGATGTC ATTGCCCAGG CCCAGTCGGG AACTGGCAAG 
ACTGCCACCT TCTCGATTGC TATCCTTCAG CAAATCGACA CGAGCATTCG CGAGTGCCAG 
GCGCTGATCC TGGCCCCCAC TCGCGAGTTG GCCACGCAGA TCCAGCGCGT GGTGATGGCG 
CTCGGCGAGT ACATGAAGGT GCACTCGCAC GCCTGCATTG GCGGCACTAA CGTGCGCGAA 
GACGCCCGCA TCTTGGAATC CGGTTGCCAT GTGGTGGTGG GCACTCCTGG CCGCGTCTAC 
GACATGATCA ACCGCAAGGT GCTGCGCACC CAGTACATCA AGCTGTTCGT GCTGGATGAG 
GCCGATGAGA TGTTGTCCCG CGGTTTCAAG GATCAGATCC AGGATGTCTT CAAGATGCTG 
CCCCCAGATG TGCAGGTCAT CCTGCTGTCC GCCACCATGC CGCCGGATGT GCTCGAAGTG 
AGCCGTTGCT TCATGCGTGA TCCCGTCAGC ATCCTGGTTA AGAAGGAGGA ACTGACCCTT 
GAGGGTATCA AGCAGTTTTA CGTCAACGTG AAGCAGGAGA ACTGGAAACT GGGCACCCTT 
TGCGATCTGT ACGATACGCT GTCCATCACC CAGTCGGTAA TCTTCTGCAA CACCCGTCGC 
AAGGTGGACC AACTGACCCA GGAGATGTCT ATCCACAACT TCACCGTCTC GGCCATGCAC 
GGCGACATGG AGCAGCGTGA TCGCGAGGTC ATCATGAAAC AATTCCGTTC GGGCTCGTCT 
CGTGTTCTGA TTACCACTGA TTTACTGGCG CGCGGTATTG ATGTGCAGCA GGTGTCGTTG 
GTCATCAACT ATGATCTGCC CTCGAACCGC GAGAACTACA TTCATAGAAT TGGTCGCGGT 
GGTCGTTTCG GTCGCAAGGG TGTTGCGATC AACTTTATTA CAGATGATGA TCGACGAATC 
CTTAAGGATA TTGAACAGTT CTACCACACA ACAATTGAGG AAATGCCTGC TAATATTGCC 
GATTTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF4A ( NM_164670.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723139.1 Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant D, mRNA.

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CloneID	OFa13851
Clone ID Related Accession (Same CDS sequence)	NM_164669.3 , NM_001273188.1 , NM_164668.3 , NM_057247.5 , NM_164670.4 , NM_001258978.3
Accession Version	NM_164670.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4A, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_164670.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGACC GAAATGAGAT ACCTCAGGAT GGCCCCGCCA GCATGGAACC CGAGGGCGTC 
ATCGAGTCCA CCTGGCACGA GGTGTACGAC AACTTCGATG ACATGAACTT GCGCGAGGAG 
TTGCTGCGCG GCATCTACGG TTATGGTTTC GAGAAGCCGT CGGCCATCCA GCAGCGCGCC 
ATCATTCCCT GTGTGAGGGG TCGCGATGTC ATTGCCCAGG CCCAGTCGGG AACTGGCAAG 
ACTGCCACCT TCTCGATTGC TATCCTTCAG CAAATCGACA CGAGCATTCG CGAGTGCCAG 
GCGCTGATCC TGGCCCCCAC TCGCGAGTTG GCCACGCAGA TCCAGCGCGT GGTGATGGCG 
CTCGGCGAGT ACATGAAGGT GCACTCGCAC GCCTGCATTG GCGGCACTAA CGTGCGCGAA 
GACGCCCGCA TCTTGGAATC CGGTTGCCAT GTGGTGGTGG GCACTCCTGG CCGCGTCTAC 
GACATGATCA ACCGCAAGGT GCTGCGCACC CAGTACATCA AGCTGTTCGT GCTGGATGAG 
GCCGATGAGA TGTTGTCCCG CGGTTTCAAG GATCAGATCC AGGATGTCTT CAAGATGCTG 
CCCCCAGATG TGCAGGTCAT CCTGCTGTCC GCCACCATGC CGCCGGATGT GCTCGAAGTG 
AGCCGTTGCT TCATGCGTGA TCCCGTCAGC ATCCTGGTTA AGAAGGAGGA ACTGACCCTT 
GAGGGTATCA AGCAGTTTTA CGTCAACGTG AAGCAGGAGA ACTGGAAACT GGGCACCCTT 
TGCGATCTGT ACGATACGCT GTCCATCACC CAGTCGGTAA TCTTCTGCAA CACCCGTCGC 
AAGGTGGACC AACTGACCCA GGAGATGTCT ATCCACAACT TCACCGTCTC GGCCATGCAC 
GGCGACATGG AGCAGCGTGA TCGCGAGGTC ATCATGAAAC AATTCCGTTC GGGCTCGTCT 
CGTGTTCTGA TTACCACTGA TTTACTGGCG CGCGGTATTG ATGTGCAGCA GGTGTCGTTG 
GTCATCAACT ATGATCTGCC CTCGAACCGC GAGAACTACA TTCATAGAAT TGGTCGCGGT 
GGTCGTTTCG GTCGCAAGGG TGTTGCGATC AACTTTATTA CAGATGATGA TCGACGAATC 
CTTAAGGATA TTGAACAGTT CTACCACACA ACAATTGAGG AAATGCCTGC TAATATTGCC 
GATTTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723139.1
CDS	458..1669
Translation	MDDRNEIPQDGPASMEPEGVIESTWHEVYDNFDDMNLREELLRGIYGYGFEKPSAIQQRAIIPCVRGRDVIAQAQSGTGKTATFSIAILQQIDTSIRECQALILAPTRELATQIQRVVMALGEYMKVHSHACIGGTNVREDARILESGCHVVVGTPGRVYDMINRKVLRTQYIKLFVLDEADEMLSRGFKDQIQDVFKMLPPDVQVILLSATMPPDVLEVSRCFMRDPVSILVKKEELTLEGIKQFYVNVKQENWKLGTLCDLYDTLSITQSVIFCNTRRKVDQLTQEMSIHNFTVSAMHGDMEQRDREVIMKQFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPSNRENYIHRIGRGGRFGRKGVAINFITDDDRRILKDIEQFYHTTIEEMPANIADLI

Target ORF information:

RefSeq Version	NM_164670.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164670.4

ORF Insert Sequence:

ATGGATGACC GAAATGAGAT ACCTCAGGAT GGCCCCGCCA GCATGGAACC CGAGGGCGTC 
ATCGAGTCCA CCTGGCACGA GGTGTACGAC AACTTCGATG ACATGAACTT GCGCGAGGAG 
TTGCTGCGCG GCATCTACGG TTATGGTTTC GAGAAGCCGT CGGCCATCCA GCAGCGCGCC 
ATCATTCCCT GTGTGAGGGG TCGCGATGTC ATTGCCCAGG CCCAGTCGGG AACTGGCAAG 
ACTGCCACCT TCTCGATTGC TATCCTTCAG CAAATCGACA CGAGCATTCG CGAGTGCCAG 
GCGCTGATCC TGGCCCCCAC TCGCGAGTTG GCCACGCAGA TCCAGCGCGT GGTGATGGCG 
CTCGGCGAGT ACATGAAGGT GCACTCGCAC GCCTGCATTG GCGGCACTAA CGTGCGCGAA 
GACGCCCGCA TCTTGGAATC CGGTTGCCAT GTGGTGGTGG GCACTCCTGG CCGCGTCTAC 
GACATGATCA ACCGCAAGGT GCTGCGCACC CAGTACATCA AGCTGTTCGT GCTGGATGAG 
GCCGATGAGA TGTTGTCCCG CGGTTTCAAG GATCAGATCC AGGATGTCTT CAAGATGCTG 
CCCCCAGATG TGCAGGTCAT CCTGCTGTCC GCCACCATGC CGCCGGATGT GCTCGAAGTG 
AGCCGTTGCT TCATGCGTGA TCCCGTCAGC ATCCTGGTTA AGAAGGAGGA ACTGACCCTT 
GAGGGTATCA AGCAGTTTTA CGTCAACGTG AAGCAGGAGA ACTGGAAACT GGGCACCCTT 
TGCGATCTGT ACGATACGCT GTCCATCACC CAGTCGGTAA TCTTCTGCAA CACCCGTCGC 
AAGGTGGACC AACTGACCCA GGAGATGTCT ATCCACAACT TCACCGTCTC GGCCATGCAC 
GGCGACATGG AGCAGCGTGA TCGCGAGGTC ATCATGAAAC AATTCCGTTC GGGCTCGTCT 
CGTGTTCTGA TTACCACTGA TTTACTGGCG CGCGGTATTG ATGTGCAGCA GGTGTCGTTG 
GTCATCAACT ATGATCTGCC CTCGAACCGC GAGAACTACA TTCATAGAAT TGGTCGCGGT 
GGTCGTTTCG GTCGCAAGGG TGTTGCGATC AACTTTATTA CAGATGATGA TCGACGAATC 
CTTAAGGATA TTGAACAGTT CTACCACACA ACAATTGAGG AAATGCCTGC TAATATTGCC 
GATTTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF4A ( NM_001258978.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245907.1 Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant E, mRNA.

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CloneID	OFa13851
Clone ID Related Accession (Same CDS sequence)	NM_164669.3 , NM_001273188.1 , NM_164668.3 , NM_057247.5 , NM_164670.4 , NM_001258978.3
Accession Version	NM_001258978.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4A, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001258978.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATGACC GAAATGAGAT ACCTCAGGAT GGCCCCGCCA GCATGGAACC CGAGGGCGTC 
ATCGAGTCCA CCTGGCACGA GGTGTACGAC AACTTCGATG ACATGAACTT GCGCGAGGAG 
TTGCTGCGCG GCATCTACGG TTATGGTTTC GAGAAGCCGT CGGCCATCCA GCAGCGCGCC 
ATCATTCCCT GTGTGAGGGG TCGCGATGTC ATTGCCCAGG CCCAGTCGGG AACTGGCAAG 
ACTGCCACCT TCTCGATTGC TATCCTTCAG CAAATCGACA CGAGCATTCG CGAGTGCCAG 
GCGCTGATCC TGGCCCCCAC TCGCGAGTTG GCCACGCAGA TCCAGCGCGT GGTGATGGCG 
CTCGGCGAGT ACATGAAGGT GCACTCGCAC GCCTGCATTG GCGGCACTAA CGTGCGCGAA 
GACGCCCGCA TCTTGGAATC CGGTTGCCAT GTGGTGGTGG GCACTCCTGG CCGCGTCTAC 
GACATGATCA ACCGCAAGGT GCTGCGCACC CAGTACATCA AGCTGTTCGT GCTGGATGAG 
GCCGATGAGA TGTTGTCCCG CGGTTTCAAG GATCAGATCC AGGATGTCTT CAAGATGCTG 
CCCCCAGATG TGCAGGTCAT CCTGCTGTCC GCCACCATGC CGCCGGATGT GCTCGAAGTG 
AGCCGTTGCT TCATGCGTGA TCCCGTCAGC ATCCTGGTTA AGAAGGAGGA ACTGACCCTT 
GAGGGTATCA AGCAGTTTTA CGTCAACGTG AAGCAGGAGA ACTGGAAACT GGGCACCCTT 
TGCGATCTGT ACGATACGCT GTCCATCACC CAGTCGGTAA TCTTCTGCAA CACCCGTCGC 
AAGGTGGACC AACTGACCCA GGAGATGTCT ATCCACAACT TCACCGTCTC GGCCATGCAC 
GGCGACATGG AGCAGCGTGA TCGCGAGGTC ATCATGAAAC AATTCCGTTC GGGCTCGTCT 
CGTGTTCTGA TTACCACTGA TTTACTGGCG CGCGGTATTG ATGTGCAGCA GGTGTCGTTG 
GTCATCAACT ATGATCTGCC CTCGAACCGC GAGAACTACA TTCATAGAAT TGGTCGCGGT 
GGTCGTTTCG GTCGCAAGGG TGTTGCGATC AACTTTATTA CAGATGATGA TCGACGAATC 
CTTAAGGATA TTGAACAGTT CTACCACACA ACAATTGAGG AAATGCCTGC TAATATTGCC 
GATTTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245907.1
CDS	71..1282
Translation	MDDRNEIPQDGPASMEPEGVIESTWHEVYDNFDDMNLREELLRGIYGYGFEKPSAIQQRAIIPCVRGRDVIAQAQSGTGKTATFSIAILQQIDTSIRECQALILAPTRELATQIQRVVMALGEYMKVHSHACIGGTNVREDARILESGCHVVVGTPGRVYDMINRKVLRTQYIKLFVLDEADEMLSRGFKDQIQDVFKMLPPDVQVILLSATMPPDVLEVSRCFMRDPVSILVKKEELTLEGIKQFYVNVKQENWKLGTLCDLYDTLSITQSVIFCNTRRKVDQLTQEMSIHNFTVSAMHGDMEQRDREVIMKQFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPSNRENYIHRIGRGGRFGRKGVAINFITDDDRRILKDIEQFYHTTIEEMPANIADLI

Target ORF information:

RefSeq Version	NM_001258978.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4A (eIF4A), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258978.3

ORF Insert Sequence:

ATGGATGACC GAAATGAGAT ACCTCAGGAT GGCCCCGCCA GCATGGAACC CGAGGGCGTC 
ATCGAGTCCA CCTGGCACGA GGTGTACGAC AACTTCGATG ACATGAACTT GCGCGAGGAG 
TTGCTGCGCG GCATCTACGG TTATGGTTTC GAGAAGCCGT CGGCCATCCA GCAGCGCGCC 
ATCATTCCCT GTGTGAGGGG TCGCGATGTC ATTGCCCAGG CCCAGTCGGG AACTGGCAAG 
ACTGCCACCT TCTCGATTGC TATCCTTCAG CAAATCGACA CGAGCATTCG CGAGTGCCAG 
GCGCTGATCC TGGCCCCCAC TCGCGAGTTG GCCACGCAGA TCCAGCGCGT GGTGATGGCG 
CTCGGCGAGT ACATGAAGGT GCACTCGCAC GCCTGCATTG GCGGCACTAA CGTGCGCGAA 
GACGCCCGCA TCTTGGAATC CGGTTGCCAT GTGGTGGTGG GCACTCCTGG CCGCGTCTAC 
GACATGATCA ACCGCAAGGT GCTGCGCACC CAGTACATCA AGCTGTTCGT GCTGGATGAG 
GCCGATGAGA TGTTGTCCCG CGGTTTCAAG GATCAGATCC AGGATGTCTT CAAGATGCTG 
CCCCCAGATG TGCAGGTCAT CCTGCTGTCC GCCACCATGC CGCCGGATGT GCTCGAAGTG 
AGCCGTTGCT TCATGCGTGA TCCCGTCAGC ATCCTGGTTA AGAAGGAGGA ACTGACCCTT 
GAGGGTATCA AGCAGTTTTA CGTCAACGTG AAGCAGGAGA ACTGGAAACT GGGCACCCTT 
TGCGATCTGT ACGATACGCT GTCCATCACC CAGTCGGTAA TCTTCTGCAA CACCCGTCGC 
AAGGTGGACC AACTGACCCA GGAGATGTCT ATCCACAACT TCACCGTCTC GGCCATGCAC 
GGCGACATGG AGCAGCGTGA TCGCGAGGTC ATCATGAAAC AATTCCGTTC GGGCTCGTCT 
CGTGTTCTGA TTACCACTGA TTTACTGGCG CGCGGTATTG ATGTGCAGCA GGTGTCGTTG 
GTCATCAACT ATGATCTGCC CTCGAACCGC GAGAACTACA TTCATAGAAT TGGTCGCGGT 
GGTCGTTTCG GTCGCAAGGG TGTTGCGATC AACTTTATTA CAGATGATGA TCGACGAATC 
CTTAAGGATA TTGAACAGTT CTACCACACA ACAATTGAGG AAATGCCTGC TAATATTGCC 
GATTTGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eIF4A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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