dsf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dsf
Entrez Gene ID	33823
Full Name	dissatisfaction
Synonyms	CG9019,DSF,Dmel\CG9019,Dsf,NR2E4
General protein information	Preferred Names dissatisfaction Names CG9019 gene product from transcript CG9019-RB CG9019-PA CG9019-PB dsf-PA dsf-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26A1-26A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057792.3	NP_477140.1	dissatisfaction, isoform A
NM_001273180.1	NP_001260109.1	dissatisfaction, isoform B

Pathways from BioSystems

Homologies

	NR2E1	XP_001094659.1
Bos taurus (cattle)	NR2E1	NP_001179582.1
Mus musculus (house mouse)	Nr2e1	NP_689415.1
Danio rerio (zebrafish)	nr2e1	NP_001003608.1
Homo sapiens (human)	NR2E1	NP_003260.1
Canis lupus familiaris (dog)	NR2E1	XP_532253.2
Caenorhabditis elegans (roundworm)	nhr-67	NP_502094.1
Drosophila melanogaster (fruit fly)	dsf	NP_477140.1
Xenopus tropicalis (tropical clawed frog)	nr2e1	XP_002937209.2
Pan troglodytes (chimpanzee)	NR2E1	XP_527467.2
Gallus gallus (chicken)	NR2E1	NP_990501.1
Rattus norvegicus (Norway rat)	Nr2e1	NP_001106668.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DSF nuclear receptor acts as a repressor in culture and in vivo
Title: DSF nuclear receptor acts as a repressor in culture and in vivo.

The following dsf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dsf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13836	NM_001273180.1 Latest version!	Drosophila melanogaster dissatisfaction (dsf), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa13836	NM_057792.3 Latest version!	Drosophila melanogaster dissatisfaction (dsf), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dsf ( NM_057792.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477140.1 Drosophila melanogaster dissatisfaction (dsf), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13836
Clone ID Related Accession (Same CDS sequence)	NM_057792.3 , NM_001273180.1
Accession Version	NM_057792.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dissatisfaction, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057792.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCACAG CGGGCGATCG CCTGTTGGAC ATTCCCTGCA AGGTGTGTGG CGATCGCAGC 
TCCGGCAAGC ACTATGGAAT CTACAGCTGC GATGGCTGCT CCGGTTTTTT CAAGCGGAGC 
ATTCATCGCA ATCGGATTTA CACCTGTAAG GCCACCGGCG ATCTCAAGGG TCGCTGTCCG 
GTGGACAAGA CCCATCGGAA TCAGTGTCGC GCCTGTCGCC TGGCCAAGTG CTTCCAGTCG 
GCCATGAACA AGGATGCTGT GCAGCACGAG CGCGGTCCTA GGAAACCCAA GTTGCACCCG 
CAACTGCATC ATCATCATCA TCATGCTGCT GCCGCCGCCG CTGCAGCGCA TCATGCAGCA 
GCCGCCCATC ACCATCACCA TCATCACCAC CACGCCCACG CAGCGGCCGC CCATCATGCG 
GCAGTGGCTG CAGCGGCTGC CTCCGGGCTG CATCACCACC ACCACGCCAT GCCCGTCTCG 
CTGGTGACCA ATGTCTCGGC CTCGTTCAAC TATACGCAGC ACATCTCCAC GCATCCGCCT 
GCTCCGGCGG CGCCACCCAG TGGCTTTCAC CTGACGGCCA GTGGCGCCCA GCAGGGACCA 
GCTCCACCAG CTGGCCACCT GCACCATGGT GGAGCCGGAC ATCAGCACGC CACGGCCTTC 
CACCATCCGG GACATGGACA CGCGCTGCCT GCCCCACATG GCGGCGTCGT CAGCAATCCC 
GGCGGCAACT CGAGCGCAAT CTCCGGCAGC GGTCCCGGCT CCACGCTGCC CTTCCCCTCG 
CACCTGCTGC ACCACAATCT GATAGCGGAG GCGGCCAGCA AGCTGCCGGG CATCACTGCC 
ACAGCCGTTG CGGCGGTGGT GTCCTCCACT AGCACGCCCT ACGCCTCGGC GGCCCAGACG 
TCGTCGCCTA GTAGCAACAA CCACAACTAC TCCTCGCCCT CGCCCAGCAA CTCCATCCAG 
TCCATCTCGA GCATTGGATC GCGCAGCGGT GGTGGCGAGG AGGGCCTCAG CCTGGGCAGC 
GAGAGTCCGC GCGTCAATGT GGAAACGGAG ACACCTTCGC CATCGAACTC GCCGCCCCTT 
AGTGCTGGTA GCATTTCGCC AGCGCCCACG TTGACCACCT CGTCGGGATC GCCGCAGCAC 
CGCCAGATGT CGCGGCACAG CCTCAGTGAG GCAACCACGC CGCCCAGCCA CGCCTCTCTC 
ATGATTTGCG CCAGCAACAA TAACAATAAC AACAATAATA ATAACAATAA TGGAGAGCAC 
AAGCAGTCGA GCTACACATC CGGATCACCG ACACCCACAA CGCCCACGCC GCCACCGCCG 
CGTTCTGGTG TAGGTTCCAC CTGCAACACG GCCAGCAGCT CCAGCGGCTT CCTGGAGCTG 
CTGCTCAGTC CGGACAAGTG CCAGGAGCTC ATCCAGTACC AGGTGCAGCA CAACACGCTG 
CTCTTCCCGC AACAGCTGTT GGACTCGCGG CTGCTCTCCT GGGAGATGCT GCAGGAGACG 
ACGGCGCGAC TGCTCTTCAT GGCGGTGCGC TGGGTCAAGT GCCTCATGCC CTTCCAGACG 
CTCTCCAAGA ACGACCAGCA TTTGCTGCTC CAGGAATCCT GGAAGGAGCT CTTCCTGCTC 
AACCTCGCCC AATGGACTAT ACCGCTGGAT CTAACGCCCA TACTGGAATC ACCGCTCATC 
CGCGAACGGG TGCTGCAGGA CGAGGCCACA CAAACGGAGA TGAAGACGAT CCAGGAGATC 
CTCTGCCGCT TCCGCCAGAT CACACCCGAC GGCAGCGAGG TGGGCTGCAT GAAGGCCATC 
GCCCTGTTCG CACCCGAAAC CGCCGGCCTG TGCGACGTGC AGCCGGTGGA GATGTTGCAG 
GATCAGGCGC AGTGCATCCT CTCCGACCAT GTGCGACTGC GCTACCCTCG CCAAGCAACC 
CGCTTCGGCA GGCTGCTGCT CCTGCTGCCC TCGCTGCGCA CCATCCGGGC GGCCACCATC 
GAGGCGCTGT TCTTCAAGGA GACCATCGGC AATGTGCCCA TTGCTCGACT GCTGCGCGAC 
ATGTACACCA TGGAACCGGC ACAGGTGGAC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477140.1
CDS	256..2331
Translation	MGTAGDRLLDIPCKVCGDRSSGKHYGIYSCDGCSGFFKRSIHRNRIYTCKATGDLKGRCPVDKTHRNQCRACRLAKCFQSAMNKDAVQHERGPRKPKLHPQLHHHHHHAAAAAAAAHHAAAAHHHHHHHHHAHAAAAHHAAVAAAAASGLHHHHHAMPVSLVTNVSASFNYTQHISTHPPAPAAPPSGFHLTASGAQQGPAPPAGHLHHGGAGHQHATAFHHPGHGHALPAPHGGVVSNPGGNSSAISGSGPGSTLPFPSHLLHHNLIAEAASKLPGITATAVAAVVSSTSTPYASAAQTSSPSSNNHNYSSPSPSNSIQSISSIGSRSGGGEEGLSLGSESPRVNVETETPSPSNSPPLSAGSISPAPTLTTSSGSPQHRQMSRHSLSEATTPPSHASLMICASNNNNNNNNNNNNGEHKQSSYTSGSPTPTTPTPPPPRSGVGSTCNTASSSSGFLELLLSPDKCQELIQYQVQHNTLLFPQQLLDSRLLSWEMLQETTARLLFMAVRWVKCLMPFQTLSKNDQHLLLQESWKELFLLNLAQWTIPLDLTPILESPLIRERVLQDEATQTEMKTIQEILCRFRQITPDGSEVGCMKAIALFAPETAGLCDVQPVEMLQDQAQCILSDHVRLRYPRQATRFGRLLLLLPSLRTIRAATIEALFFKETIGNVPIARLLRDMYTMEPAQVDK

Target ORF information:

RefSeq Version	NM_057792.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dissatisfaction (dsf), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057792.3

ORF Insert Sequence:

ATGGGCACAG CGGGCGATCG CCTGTTGGAC ATTCCCTGCA AGGTGTGTGG CGATCGCAGC 
TCCGGCAAGC ACTATGGAAT CTACAGCTGC GATGGCTGCT CCGGTTTTTT CAAGCGGAGC 
ATTCATCGCA ATCGGATTTA CACCTGTAAG GCCACCGGCG ATCTCAAGGG TCGCTGTCCG 
GTGGACAAGA CCCATCGGAA TCAGTGTCGC GCCTGTCGCC TGGCCAAGTG CTTCCAGTCG 
GCCATGAACA AGGATGCTGT GCAGCACGAG CGCGGTCCTA GGAAACCCAA GTTGCACCCG 
CAACTGCATC ATCATCATCA TCATGCTGCT GCCGCCGCCG CTGCAGCGCA TCATGCAGCA 
GCCGCCCATC ACCATCACCA TCATCACCAC CACGCCCACG CAGCGGCCGC CCATCATGCG 
GCAGTGGCTG CAGCGGCTGC CTCCGGGCTG CATCACCACC ACCACGCCAT GCCCGTCTCG 
CTGGTGACCA ATGTCTCGGC CTCGTTCAAC TATACGCAGC ACATCTCCAC GCATCCGCCT 
GCTCCGGCGG CGCCACCCAG TGGCTTTCAC CTGACGGCCA GTGGCGCCCA GCAGGGACCA 
GCTCCACCAG CTGGCCACCT GCACCATGGT GGAGCCGGAC ATCAGCACGC CACGGCCTTC 
CACCATCCGG GACATGGACA CGCGCTGCCT GCCCCACATG GCGGCGTCGT CAGCAATCCC 
GGCGGCAACT CGAGCGCAAT CTCCGGCAGC GGTCCCGGCT CCACGCTGCC CTTCCCCTCG 
CACCTGCTGC ACCACAATCT GATAGCGGAG GCGGCCAGCA AGCTGCCGGG CATCACTGCC 
ACAGCCGTTG CGGCGGTGGT GTCCTCCACT AGCACGCCCT ACGCCTCGGC GGCCCAGACG 
TCGTCGCCTA GTAGCAACAA CCACAACTAC TCCTCGCCCT CGCCCAGCAA CTCCATCCAG 
TCCATCTCGA GCATTGGATC GCGCAGCGGT GGTGGCGAGG AGGGCCTCAG CCTGGGCAGC 
GAGAGTCCGC GCGTCAATGT GGAAACGGAG ACACCTTCGC CATCGAACTC GCCGCCCCTT 
AGTGCTGGTA GCATTTCGCC AGCGCCCACG TTGACCACCT CGTCGGGATC GCCGCAGCAC 
CGCCAGATGT CGCGGCACAG CCTCAGTGAG GCAACCACGC CGCCCAGCCA CGCCTCTCTC 
ATGATTTGCG CCAGCAACAA TAACAATAAC AACAATAATA ATAACAATAA TGGAGAGCAC 
AAGCAGTCGA GCTACACATC CGGATCACCG ACACCCACAA CGCCCACGCC GCCACCGCCG 
CGTTCTGGTG TAGGTTCCAC CTGCAACACG GCCAGCAGCT CCAGCGGCTT CCTGGAGCTG 
CTGCTCAGTC CGGACAAGTG CCAGGAGCTC ATCCAGTACC AGGTGCAGCA CAACACGCTG 
CTCTTCCCGC AACAGCTGTT GGACTCGCGG CTGCTCTCCT GGGAGATGCT GCAGGAGACG 
ACGGCGCGAC TGCTCTTCAT GGCGGTGCGC TGGGTCAAGT GCCTCATGCC CTTCCAGACG 
CTCTCCAAGA ACGACCAGCA TTTGCTGCTC CAGGAATCCT GGAAGGAGCT CTTCCTGCTC 
AACCTCGCCC AATGGACTAT ACCGCTGGAT CTAACGCCCA TACTGGAATC ACCGCTCATC 
CGCGAACGGG TGCTGCAGGA CGAGGCCACA CAAACGGAGA TGAAGACGAT CCAGGAGATC 
CTCTGCCGCT TCCGCCAGAT CACACCCGAC GGCAGCGAGG TGGGCTGCAT GAAGGCCATC 
GCCCTGTTCG CACCCGAAAC CGCCGGCCTG TGCGACGTGC AGCCGGTGGA GATGTTGCAG 
GATCAGGCGC AGTGCATCCT CTCCGACCAT GTGCGACTGC GCTACCCTCG CCAAGCAACC 
CGCTTCGGCA GGCTGCTGCT CCTGCTGCCC TCGCTGCGCA CCATCCGGGC GGCCACCATC 
GAGGCGCTGT TCTTCAAGGA GACCATCGGC AATGTGCCCA TTGCTCGACT GCTGCGCGAC 
ATGTACACCA TGGAACCGGC ACAGGTGGAC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dsf ( NM_001273180.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260109.1 Drosophila melanogaster dissatisfaction (dsf), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13836
Clone ID Related Accession (Same CDS sequence)	NM_057792.3 , NM_001273180.1
Accession Version	NM_001273180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dissatisfaction, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCACAG CGGGCGATCG CCTGTTGGAC ATTCCCTGCA AGGTGTGTGG CGATCGCAGC 
TCCGGCAAGC ACTATGGAAT CTACAGCTGC GATGGCTGCT CCGGTTTTTT CAAGCGGAGC 
ATTCATCGCA ATCGGATTTA CACCTGTAAG GCCACCGGCG ATCTCAAGGG TCGCTGTCCG 
GTGGACAAGA CCCATCGGAA TCAGTGTCGC GCCTGTCGCC TGGCCAAGTG CTTCCAGTCG 
GCCATGAACA AGGATGCTGT GCAGCACGAG CGCGGTCCTA GGAAACCCAA GTTGCACCCG 
CAACTGCATC ATCATCATCA TCATGCTGCT GCCGCCGCCG CTGCAGCGCA TCATGCAGCA 
GCCGCCCATC ACCATCACCA TCATCACCAC CACGCCCACG CAGCGGCCGC CCATCATGCG 
GCAGTGGCTG CAGCGGCTGC CTCCGGGCTG CATCACCACC ACCACGCCAT GCCCGTCTCG 
CTGGTGACCA ATGTCTCGGC CTCGTTCAAC TATACGCAGC ACATCTCCAC GCATCCGCCT 
GCTCCGGCGG CGCCACCCAG TGGCTTTCAC CTGACGGCCA GTGGCGCCCA GCAGGGACCA 
GCTCCACCAG CTGGCCACCT GCACCATGGT GGAGCCGGAC ATCAGCACGC CACGGCCTTC 
CACCATCCGG GACATGGACA CGCGCTGCCT GCCCCACATG GCGGCGTCGT CAGCAATCCC 
GGCGGCAACT CGAGCGCAAT CTCCGGCAGC GGTCCCGGCT CCACGCTGCC CTTCCCCTCG 
CACCTGCTGC ACCACAATCT GATAGCGGAG GCGGCCAGCA AGCTGCCGGG CATCACTGCC 
ACAGCCGTTG CGGCGGTGGT GTCCTCCACT AGCACGCCCT ACGCCTCGGC GGCCCAGACG 
TCGTCGCCTA GTAGCAACAA CCACAACTAC TCCTCGCCCT CGCCCAGCAA CTCCATCCAG 
TCCATCTCGA GCATTGGATC GCGCAGCGGT GGTGGCGAGG AGGGCCTCAG CCTGGGCAGC 
GAGAGTCCGC GCGTCAATGT GGAAACGGAG ACACCTTCGC CATCGAACTC GCCGCCCCTT 
AGTGCTGGTA GCATTTCGCC AGCGCCCACG TTGACCACCT CGTCGGGATC GCCGCAGCAC 
CGCCAGATGT CGCGGCACAG CCTCAGTGAG GCAACCACGC CGCCCAGCCA CGCCTCTCTC 
ATGATTTGCG CCAGCAACAA TAACAATAAC AACAATAATA ATAACAATAA TGGAGAGCAC 
AAGCAGTCGA GCTACACATC CGGATCACCG ACACCCACAA CGCCCACGCC GCCACCGCCG 
CGTTCTGGTG TAGGTTCCAC CTGCAACACG GCCAGCAGCT CCAGCGGCTT CCTGGAGCTG 
CTGCTCAGTC CGGACAAGTG CCAGGAGCTC ATCCAGTACC AGGTGCAGCA CAACACGCTG 
CTCTTCCCGC AACAGCTGTT GGACTCGCGG CTGCTCTCCT GGGAGATGCT GCAGGAGACG 
ACGGCGCGAC TGCTCTTCAT GGCGGTGCGC TGGGTCAAGT GCCTCATGCC CTTCCAGACG 
CTCTCCAAGA ACGACCAGCA TTTGCTGCTC CAGGAATCCT GGAAGGAGCT CTTCCTGCTC 
AACCTCGCCC AATGGACTAT ACCGCTGGAT CTAACGCCCA TACTGGAATC ACCGCTCATC 
CGCGAACGGG TGCTGCAGGA CGAGGCCACA CAAACGGAGA TGAAGACGAT CCAGGAGATC 
CTCTGCCGCT TCCGCCAGAT CACACCCGAC GGCAGCGAGG TGGGCTGCAT GAAGGCCATC 
GCCCTGTTCG CACCCGAAAC CGCCGGCCTG TGCGACGTGC AGCCGGTGGA GATGTTGCAG 
GATCAGGCGC AGTGCATCCT CTCCGACCAT GTGCGACTGC GCTACCCTCG CCAAGCAACC 
CGCTTCGGCA GGCTGCTGCT CCTGCTGCCC TCGCTGCGCA CCATCCGGGC GGCCACCATC 
GAGGCGCTGT TCTTCAAGGA GACCATCGGC AATGTGCCCA TTGCTCGACT GCTGCGCGAC 
ATGTACACCA TGGAACCGGC ACAGGTGGAC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260109.1
CDS	256..2331
Translation	MGTAGDRLLDIPCKVCGDRSSGKHYGIYSCDGCSGFFKRSIHRNRIYTCKATGDLKGRCPVDKTHRNQCRACRLAKCFQSAMNKDAVQHERGPRKPKLHPQLHHHHHHAAAAAAAAHHAAAAHHHHHHHHHAHAAAAHHAAVAAAAASGLHHHHHAMPVSLVTNVSASFNYTQHISTHPPAPAAPPSGFHLTASGAQQGPAPPAGHLHHGGAGHQHATAFHHPGHGHALPAPHGGVVSNPGGNSSAISGSGPGSTLPFPSHLLHHNLIAEAASKLPGITATAVAAVVSSTSTPYASAAQTSSPSSNNHNYSSPSPSNSIQSISSIGSRSGGGEEGLSLGSESPRVNVETETPSPSNSPPLSAGSISPAPTLTTSSGSPQHRQMSRHSLSEATTPPSHASLMICASNNNNNNNNNNNNGEHKQSSYTSGSPTPTTPTPPPPRSGVGSTCNTASSSSGFLELLLSPDKCQELIQYQVQHNTLLFPQQLLDSRLLSWEMLQETTARLLFMAVRWVKCLMPFQTLSKNDQHLLLQESWKELFLLNLAQWTIPLDLTPILESPLIRERVLQDEATQTEMKTIQEILCRFRQITPDGSEVGCMKAIALFAPETAGLCDVQPVEMLQDQAQCILSDHVRLRYPRQATRFGRLLLLLPSLRTIRAATIEALFFKETIGNVPIARLLRDMYTMEPAQVDK

Target ORF information:

RefSeq Version	NM_001273180.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dissatisfaction (dsf), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273180.1

ORF Insert Sequence:

ATGGGCACAG CGGGCGATCG CCTGTTGGAC ATTCCCTGCA AGGTGTGTGG CGATCGCAGC 
TCCGGCAAGC ACTATGGAAT CTACAGCTGC GATGGCTGCT CCGGTTTTTT CAAGCGGAGC 
ATTCATCGCA ATCGGATTTA CACCTGTAAG GCCACCGGCG ATCTCAAGGG TCGCTGTCCG 
GTGGACAAGA CCCATCGGAA TCAGTGTCGC GCCTGTCGCC TGGCCAAGTG CTTCCAGTCG 
GCCATGAACA AGGATGCTGT GCAGCACGAG CGCGGTCCTA GGAAACCCAA GTTGCACCCG 
CAACTGCATC ATCATCATCA TCATGCTGCT GCCGCCGCCG CTGCAGCGCA TCATGCAGCA 
GCCGCCCATC ACCATCACCA TCATCACCAC CACGCCCACG CAGCGGCCGC CCATCATGCG 
GCAGTGGCTG CAGCGGCTGC CTCCGGGCTG CATCACCACC ACCACGCCAT GCCCGTCTCG 
CTGGTGACCA ATGTCTCGGC CTCGTTCAAC TATACGCAGC ACATCTCCAC GCATCCGCCT 
GCTCCGGCGG CGCCACCCAG TGGCTTTCAC CTGACGGCCA GTGGCGCCCA GCAGGGACCA 
GCTCCACCAG CTGGCCACCT GCACCATGGT GGAGCCGGAC ATCAGCACGC CACGGCCTTC 
CACCATCCGG GACATGGACA CGCGCTGCCT GCCCCACATG GCGGCGTCGT CAGCAATCCC 
GGCGGCAACT CGAGCGCAAT CTCCGGCAGC GGTCCCGGCT CCACGCTGCC CTTCCCCTCG 
CACCTGCTGC ACCACAATCT GATAGCGGAG GCGGCCAGCA AGCTGCCGGG CATCACTGCC 
ACAGCCGTTG CGGCGGTGGT GTCCTCCACT AGCACGCCCT ACGCCTCGGC GGCCCAGACG 
TCGTCGCCTA GTAGCAACAA CCACAACTAC TCCTCGCCCT CGCCCAGCAA CTCCATCCAG 
TCCATCTCGA GCATTGGATC GCGCAGCGGT GGTGGCGAGG AGGGCCTCAG CCTGGGCAGC 
GAGAGTCCGC GCGTCAATGT GGAAACGGAG ACACCTTCGC CATCGAACTC GCCGCCCCTT 
AGTGCTGGTA GCATTTCGCC AGCGCCCACG TTGACCACCT CGTCGGGATC GCCGCAGCAC 
CGCCAGATGT CGCGGCACAG CCTCAGTGAG GCAACCACGC CGCCCAGCCA CGCCTCTCTC 
ATGATTTGCG CCAGCAACAA TAACAATAAC AACAATAATA ATAACAATAA TGGAGAGCAC 
AAGCAGTCGA GCTACACATC CGGATCACCG ACACCCACAA CGCCCACGCC GCCACCGCCG 
CGTTCTGGTG TAGGTTCCAC CTGCAACACG GCCAGCAGCT CCAGCGGCTT CCTGGAGCTG 
CTGCTCAGTC CGGACAAGTG CCAGGAGCTC ATCCAGTACC AGGTGCAGCA CAACACGCTG 
CTCTTCCCGC AACAGCTGTT GGACTCGCGG CTGCTCTCCT GGGAGATGCT GCAGGAGACG 
ACGGCGCGAC TGCTCTTCAT GGCGGTGCGC TGGGTCAAGT GCCTCATGCC CTTCCAGACG 
CTCTCCAAGA ACGACCAGCA TTTGCTGCTC CAGGAATCCT GGAAGGAGCT CTTCCTGCTC 
AACCTCGCCC AATGGACTAT ACCGCTGGAT CTAACGCCCA TACTGGAATC ACCGCTCATC 
CGCGAACGGG TGCTGCAGGA CGAGGCCACA CAAACGGAGA TGAAGACGAT CCAGGAGATC 
CTCTGCCGCT TCCGCCAGAT CACACCCGAC GGCAGCGAGG TGGGCTGCAT GAAGGCCATC 
GCCCTGTTCG CACCCGAAAC CGCCGGCCTG TGCGACGTGC AGCCGGTGGA GATGTTGCAG 
GATCAGGCGC AGTGCATCCT CTCCGACCAT GTGCGACTGC GCTACCCTCG CCAAGCAACC 
CGCTTCGGCA GGCTGCTGCT CCTGCTGCCC TCGCTGCGCA CCATCCGGGC GGCCACCATC 
GAGGCGCTGT TCTTCAAGGA GACCATCGGC AATGTGCCCA TTGCTCGACT GCTGCGCGAC 
ATGTACACCA TGGAACCGGC ACAGGTGGAC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dsf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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