bchs cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bchs
Entrez Gene ID	33819
Full Name	blue cheese
Synonyms	Alfy,BcDNA:SD12984,Bchs,Beach1,CG14001,CG9011,DBEACH1,Dmel\CG14001,EP2299,alfy,bchd
General protein information	Preferred Names blue cheese Names CG14001 gene product from transcript CG14001-RA Beach1 Beached1 CG14001-PA bchs-PA beached beached 1 dbeach1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26A1-26A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_135124.2	NP_608968.2	blue cheese

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	WDFY3	XP_004941192.1
	WDFY3	XP_002804111.1
Rattus norvegicus (Norway rat)	Wdfy3	NP_001164022.1
Xenopus tropicalis (tropical clawed frog)	wdfy3	XP_002937925.2
Homo sapiens (human)	WDFY3	NP_055806.2
Bos taurus (cattle)	WDFY3	XP_002707872.1
Drosophila melanogaster (fruit fly)	bchs	NP_608968.2
Pan troglodytes (chimpanzee)	WDFY3	XP_001151618.1
Canis lupus familiaris (dog)	WDFY3	XP_544963.3
Mus musculus (house mouse)	Wdfy3	NP_766470.2
Danio rerio (zebrafish)	wdfy3	XP_005165447.1
Caenorhabditis elegans (roundworm)	C26H9A.2	NP_001255658.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

blue cheese mutant brains exhibit an elevation in total ceramide levels.
Title: Ceramides And Stress Signalling Intersect With Autophagic Defects In Neurodegenerative Drosophila blue cheese (bchs) Mutants.

Anterograde transport of lysosomes toward synaptic termini is a key factor in preventing motor neuron degeneration and that Bchs reveals a functional link between the lysosomal degradative pathway and transport.
Title: The Drosophila BEACH family protein, blue cheese, links lysosomal axon transport with motor neuron degeneration.

Mutations in the Drosophila gene blue cheese (bchs) lead to reduced adult life span, increased neuronal death, and widespread nerouvos system degeneration
Title: Genetic modifiers of the Drosophila blue cheese gene link defects in lysosomal transport with decreased life span and altered ubiquitinated-protein profiles.

Consistent with a role for Bchs in modulating Rab11-dependent trafficking, Bchs protein is associated with vesicles and extensively colocalized with Rab11 at the neuromuscular junction.
Title: Bchs, a BEACH domain protein, antagonizes Rab11 in synapse morphogenesis and other developmental events.

blue cheese mutations define a novel, conserved gene involved in progressive neural degeneration.
Title: blue cheese mutations define a novel, conserved gene involved in progressive neural degeneration.

The following bchs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bchs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13833	NM_135124.2 Latest version!	Drosophila melanogaster blue cheese (bchs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bchs ( NM_135124.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608968.2 Drosophila melanogaster blue cheese (bchs), mRNA.

Price & Availability↑

CloneID	OFa13833
Clone ID Related Accession (Same CDS sequence)	NM_135124.2
Accession Version	NM_135124.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	blue cheese
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135124.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATGTAA TGCGTAAGCT GCGCGGAGCG GCCAGCGCAG GCAGCGTCAG CGGCAGCAGT 
AGCGGAACCT CGACGGCGAA CAACAGTTCA CCAGGGAGCA GCAGAACGGA TGCTGGTGCA 
CCGACCGCTG CAAATGGACG GAATGCGGAG GAGGCGCTGA TGGACGCCCG CGTCCAAGTG 
AGCCTCACCA CGCTAAAGAA GCTATTCAAC GAATACACTC ATCCGCGGGA GCCGCTGAGC 
GAACAGGAGC GCGATGACAA GCTCTACGAG ATGCTGCCCC TCTTCTGCAA AGTCTTCAGC 
AGCTGTCCGT CAAACGACAT GAGCGAAAAG TTCTGGGACG TGGTCGCCTT CTGCCAGCAA 
GTGTCCCGTC TCATGGTCAG CGAAATCCGC AAGCGCGCCT CCAACCAGAG CACAGAGGCG 
GCATCCATTG CCATAGTCAA GTTTCTCGAG GTGGAAACCA CCGAGGAAAC CAGCAGCGGA 
TGGATGCTTT TGGCTACCCT CAATTTGTTG GCCAATGGAG ACGTATCTCT CATACAGGTC 
ATGACTGCTG CGGCGGTTCC CTCTACTTTA GTGAAGTGCT TGTACTTGTT CTTCGACCTG 
CCCATAGTTG AGGACGATGA GCCTTCAGCT GACGGTGGAG CAGTAAGTGA GTTTAATGCC 
CATGAAAGGC GGACGCTTCT GCAGAAGGTG TTTGTGCAGC TGCTGGTCAA GCTGTGCTCG 
TATCCGTATC CCGCTGAGGA ACTAGCTCGC ATGGACGACT TGACGCTGCT ATTCTCGGCC 
ATCACATCGC CATGTCCCAT CCACAATATT GTATGGCGCA AGAACGCAGC TGAAATCCTG 
ACCACAATAT CAAGGAATGG CCTAACCGAT GCCGTAGTTA GCTATATACA TTCCAAGGGC 
TGCATGGCAC TTTGCGTGGA CAATATGCAG CGCCTGACGT TTGGAAATCC TTTGGAGATC 
GTTGAAATGT TCGTCACCGT GTTCTGCTTT CTCAAGGACT CCAGTCAGGT ATCCCAGATT 
TTGATGGATG ATTTTCGTGC ATCTCAAGGT TACGTGTTTC TCAGTGATTT TCTACTCAAG 
TTTGACAACA ATCGTAGCCA ATCATTGGAG ATTCAAGCGG CCATTCGAAA TCTGGTACTG 
ATGATCTCTT CGTTGTGCAT GTGTGGCTTT TACGAGCTTC GACCGCCAGC CTCGCAGTTT 
AATACGGCTT TCAAACTGCA GAACTTCCAG CTCCCACAGG CCACCTCACG AGAGACATGC 
GTGCGAAATG TGTACGCCTT CCAGGTGCTG CAGAACGTGT TCCTTAAGTC AACGACGCCT 
GCGCTCTGCT GCACAATACT GGACGCCATT TCGCGGGTGT ACCACTCGGA AAATGCCAAC 
TACTTTATCC TGGAATCGGA GCAAACGCTT AGTTCGTTCG CAGAGCGGAT ACACATGAAA 
AGCCCGCAAA TACAAGAAAA ATTCTACGAT CTGCTGGAGT TCATTGTCTT CCAACTGAAC 
TTCGTGCCGT GCAAGGAGCT GATCAGCCTT TCGCTATTGC TCAAGCACAA CCAGTCGACG 
TCCTGCAGCA TTCTTTGCTT GAAGACACTG TTAAACATTC TCCGGCACAA CGCTGTATTT 
AAAGATGTCT ACCGCGAAGT GGGGATTCTG GAGATCTTTG TTGGCTGTCT AACACGCTAT 
GCAGCTCATG TGCAGAAAAT AACCAAGGGA GATGAGTCAG TGGTGGTGGA GCTGGAAAAT 
GAATCCGAAG AAGAACTGTT CGACACACTA GGAAAGCATG TTCTGGAGGC CCTCACCATG 
CTGCTGGGAG GTGGTGCCAG CAACAATGCC CAACTATTTC GTGAGTACGG TGGCGCCAAG 
TGCGTCCATG AGTTGGTGAA GTTCAAGCAC TGCCGACCGC AGGCTCTCGG AATCGTAAGG 
GAGCTAATCC TGTCCGCAGG TGGAGACGAC GACATGCTGC ACATACTCTC ACTGATGCAC 
AGCGTCAGCC CACTACAAGT GGAGTTTAAG ATTCAGATAC TAAACATGTT GCTCGGCTGC 
CTAAAGGACT CTCATCGCAC ACGGACCGTA TTCCGCAAAG TGGGCGGATT CGTTTACGTG 
ACCAGTGTAT TTGTGTCTCT GGATGGCAGC ATGGCCCTCC CTCAACCGGA CATTCCACAA 
CAGGATCTCA TTCTGCTGCT GCAAATAGTC TTCCAGACTC TAGCCACCGC TATGAGATTT 
GAACCGGCCA ACGCCAAGTT CTTTCACCAG GAGATTAGCA GCAGCTCATT GTGCGACACA 
CTTCGTCTGC TGGGATGCTT TGGTGGATCG AGTGCGTTAC AGGACTACAC GGGCACCTAC 
GAGCCGCAGC AGTCGCTCCT CAAGTATTAT CATGAGATTT TCAGCGGTGA TATTCTAAGC 
GTCAGTTTCT CTGAAGACGT TCCATACCCG CTGTCATACG TGTGCATCGT ATTTCGGCTA 
CTATACAGTA TTGCCCTAGA TAATTTTGAG GCTCCAAATC TAAGCGGCAT TATTACACTC 
TTCAGCGATC CTCCGACAAC TTTGCGATCG CCGAGTAAAG AGCTGGCGCC ACCCGTGCAT 
CCCAGCCAGC TGAATCTCAC GCAGCCCAGT CCGGAGCCTA GAATAGTGCA CCCAGGTGTG 
GTTCTTTGCA TGCTTCAGCT GCTCCCCGCC GTGGAGTACG ACATGGCTCC TTTACAGGCG 
GTGCAACTGC AAGTATATCT AAGCGAAATC ATCAAGTCCT TGGTGCGAAG CGAACGCAAC 
CAGCAGATAA TGTGTGACCA CGGTCTGGCA GAGAAACTGC TTAAGCTGAC GCGAAGAGCC 
CTGGCGGAGG AGTCGCATCC GTTGCACGTG CCAATGCAGT ACATTCTGGA GCGTCTTGCC 
GCTCAAGCAT TGCAACCTAC CGAATTGCGC CAGTTCCTGC GGCTGGGTGA GCCGCTCTCG 
TGTGCGGATA TAGATCTTCA GCAGCCATAC AAGCTGGGTG GGCCCGTTCC GCTGACTCGT 
ATCAAGACTC TGGTGTCTAT GACAACGCCC CGTGACTTCC GAGCCCATGG TTCGAGCACG 
CTCCCTCCGT TCGTGGAGCT GGACATGTCC GCCGAGGGAT TCGGTTGCTT GTACCTGCCT 
TCATTGGCGC CACAGGCAAC GGCCACCGCT GGAGGAACCA TCGATGCAAA CAGCATTGGT 
GGCATCGGGG CTGGCGATCG CATCTTTCCA CCACAAACTG GACTCACATA CTCCACCTGG 
TTCTGTGTAG AAAAGTTCTC AGATCCCAAG ACGGACCCTC ACTGTGTTCG GCTTCTTACT 
TTGGTTAGGA CCATCCACAA TCCACGAGAG GAAAACTTAG CATGTTTATC CATTTTGCTT 
TCCGCACGGG ACAAGGCGAT CGTCGTCTCG ACGCAGGAGA CGTTGGTCAC ACCCAGAAAA 
AGTATTGGTG ACTGGGAACC CGAAGGATCG GATGACGGTA TCGCTCGCAT ATGGTGTCCT 
GATCTTTTGC ACGAAGGTCA GTGGCACAAC CTGGTCGTTG TACTCAACAG GGCTGTGCTG 
AAAAATTCGA GTCTGTTCCT CTATTTGGAC GGGGTTCCAA TGCATACTCA AAAGCTGCAC 
TATATTGCCC AGCATCCCGC AGCAGGAAAC GCCAGTTTGA CGTCGCCCAC ACAGATTTTT 
GGATATATCG GTACGCCACC AATATGGCGG CGTTACTCAC GTCTCTGTTG GAAGCAGGGC 
GTTTGTCACC TTATCGAGGA TGTGCTAACG CAGCAAACAG TTCAGACAAT ATACCAACTG 
GGGCCTCACT ACATGGGCTC TTTGCAAGCT CCGCAACTTG GCAAGCAGTC GGAGTCATTG 
GCGCCTTTGG TGCCAGAGGA TCGCGTCCTC CTGGGTCTTA ATGCTAAAGC GGTGTCCAAG 
CTGACACTAG TCAAAATCCG CAAAGTTTAC AGCCGAGCGG ACAACAAAAG CATTGCCAAG 
CAGCTAAACA TGAACTCGCA CGAGAATGCT ACGCCCATAA AAATTCTTCA CAACTCTGCA 
GGGCATCTTG CAGGAGCTGG ACGCTCCCTT GGCGGCGTGG TCGTCGGTTA TCTGGGCGTC 
AGAGTATTCA GTCCACATCC CGTCTCAGCC ATGATCGACA CAGTTGGTGG CTGCAACGTG 
CTCCTCGGTA TCATCGCCAT GGCTCAAGAC GTGGAATCCC TATACGCAGG TGTCAAAGCT 
TTGACCTGCG TGGTGCGAAG CAATCGCGCC GCTCAAGCCG AGATGGACAG AAAACGATGC 
TACCAGACAT TGGGCATGTT CTTTAAAAAG AAAAAACATC TGCTAAACTC GCACATCTTG 
CACCTGACCT TTGGTCTAGT AGGCACGGTT AACAGCGGAC AAGACATGTC GGCTATTCCA 
AATGTGACAG CCTTCCAAGA TCTTTTGTGT GATCTTGAGA TTTGGCATAA TGCACCAAAC 
GGACTTCTGC GCTCCCTGCT AGAACATCTA TTGGAGCTAG TGGTCGAGTC CAGTGACAAG 
AAGCAGAACG TGAAGATCAT GCGCGACCTC CAGCTTCTAG TGAAATTGCT GCACATCATT 
ACCCAAATCC AAGACCACTC CACACGAGAA ATTCTCTTCA GTCTGCTGGA GACTCTACTG 
GGCGGTCAGC CGAGGCACAC CGATCTACTG CTTTTCGGTC AGTATGTGGC GGCCAAGTTG 
CCACATGCCC ATTCTGGAGG CTTGGAACGC GCTGTGCTGC TGCCCAGCAT GAAAAATCCT 
GCCGAGGATC AAGACGGTGG CGTGGCCCAG AACATATACC TACGTAATCG CTGTCTATCG 
CTGCTCCACG GACTGCTATT CACACCGCGA AACACCGTGA ACTATGTAAT CTGCGACGAC 
ATTTCCAAAA CATTGGGCAT GGATTGGTTG CTACTCTTCA TGCAACCGCA CGTCCACTTC 
ACCACTGTAA TCATTGCAGT GCGCATCCTG GTCGTAATCT GTGCTAACGA AAGTTTTCTT 
GTGCGATTCC GGGATGCCAC CCACAACGGC GGTTATCTTC GGTTTACCGA GATGGTCTCT 
CAGCGAAAGA TGCTGGGACT CGGTGCCCAG CAGCTTAACC AGCGACCAAC GAATGGCACT 
GGTACAGTCA TTGTGGCCAC CCCACAGAAT ACCATACAAC ATCTGCCCAC GCAGATCGCA 
GGAGAGGTGC GAGCAGCGGC CTTAAACATA CCAGGTTTCC AGCTTCTTGA ATGGCTAATG 
AATCATCACC TGGACGTTCC GGAGTTGTAC TTCCTAATCA CCGCCCTGAT TATGGGACAA 
CCTGTAAAGG TGTTGGCTAC TGAGCACACA AAGTTCGATC TTGACCGCGT CTGGTCGTTC 
CTCTGGGGAG CTCCTGTGTC AGCCAACACA CAGCTACCAA AGCTCAACAT TTGTCCCGAG 
GGCGTATGCG TACTCCTGGC AATGGTGCGG GGAATCGTTC ATGGTGGAGA GTGCGCTCCA 
TGGCTGCACA GCCACCCGGA AACAATTATC CAGCTCCTGT TTAGCTTGTA CCAAAACCTC 
ACCGACTTTG CGCCTGTGAT GATGACGGGC GATGTGGTCA CCTCGCTAGT AGCTGTGCTA 
TTTCCGTTGG CATCTCGTCC TGCTGACTCT GAGCCAAACA GTGGAGCATC GACACCCACA 
GACGATACGG GAAGCAGCTT TGCTTTGCCA CCGCCGGAAA CACTTCATGG AGCTCCGCAG 
CCCAAGCTAA CAGCTCATCC GGTGTGTAAT TGCATTATCG ACTTCCTGCG CGTTATTGTC 
GTGGACTCTC TCGGCCTCAA CATGCAGGGT AAACCTACTC CGGTAATCGA TCTCGTGCTG 
GACGCAGCAC CAGAATCGGC TGAACTACCC CTCCAGGTGC AGTATCAGAC CCAGATAATT 
ATCGCCCTAA TGGATCACCT ACTGGCCGCC GACGTGCTTG TAGGCGAACA GGCAGCACTC 
CCGCTGGTGC CTCTCTTGCA GAGCCAAATG CAATACATCG CCCCAAACGT GTTTTACCTG 
ACCGCCCGCA TCGTAGATAA ACTATGGCAA GGTTGCCTCG CCCGCAATCC ACACGACATT 
TTCGATTTTG TCATAAAACT GATCGTACAG GCGAAGCGTC GGTCGTCCTC GTTGTCATTG 
GAGCATCTTC ATCACTCGCT GAACCGCAGC ATCCTCTTCC TGCTTTCCCG ACCCACCGAT 
GACTCGCGAG CTGACCAGAT GAGCGTGCTG GAGGCACTGC ACAAGATCAT TCAGCACCGC 
CTGCTTATTT TCGGAGCGGG CAACCACGAG TTGGAATTCA TTGGCTGCCT CACATACTGT 
TTGCTGCAGT TGACTGCCGA TATGAAAATT ATATTGGAGC CGGCAACCAG CCGAAACACT 
ACATGGCACG TAAATCCGCA GACGGAGACG GCAGAGCCCA AGGATGAAGA TCTCAACCAG 
TTACAGGGTC GGAATCTGAT CGTTGGCGCT GCTTTTCGAG TTTGGGAGGA GCTATACGTA 
TGCAAGAAGC CTGCCATTGA GGAGGTATTT AAAGTCTCCC TAACATCCCC ACCGCCGAAC 
TCCAAAGCAC CTGATCTGCA GACCACGCGA GAGCAGGTCA TGGAGCTGGC CTCTAAACTA 
TGGTTCAATT ATGTGGAGGC AGAACGTAAG GCCACATATC GTGCGCCCTG GGAGCTGCAC 
ACCCAGATCC AGTCCAAAAT CCAGAAGGTT ACTGGGGGAC TGTCCCGTTT GACTAGCAGA 
ACAAAAACCA AAAAGGAGGA GCTGGTACGA ACCAGGTCGA CCTTAACTCG CGAGGCAGCC 
TATGAGTCCA CCGGCATCCA TGTGCAGCTG ATAAAGGATC TTTTGGATTT GCGTGCGAAG 
CAGTACCAGC AGATGTTGCA GCATACTCAG CGCTACGTCT ATCAGGACTG GGTGCAGTCG 
GAGATGGAGC TTACCCGGGA GCGCGGTCTG TGGGGTCCCA CTGGCAGCTG CTCATTGGAT 
AAGTGGATTT TAGACACCAC CGAAGGACCC CACCGTATGC GCAAGAAAAC GATGCGTAAT 
GATGTCTTCT ACCTGCACTA TCCATACAGA CCGGAGTTGG AGCTGGCGGA CAATCGGCAG 
CTGAAGTACA AGGTGGCCTC CAGTCTAGAT AGCAAGACGT ACGCACTGCA CGGACCGCAG 
CAGCCACGCA TACTGGCCGA GGCGGGGGAG CACCATGCGA TGCAACAGCA GAGTTCTCTG 
GAAGCGGTGC AATCGCATCG GTTGGAGACC AGCAGTTCGA CATCAACGCC ACCACCCATG 
GTTTTGCCCA AGCTTGTGGG ACACGGATCA ACACCCTGTC CACAGGAGTC GGTGGACGGC 
AATGCTCCAG AGGATGACGA AGAAGAAGAG GATACTTCGA TGACCAGCGA TAATGAGACG 
TTTTTGAGGC TACTAGAGGA GCAGGAGAAG ATTAGTTTTA TGTTCCGTTG TGCAAGAGTC 
CAAGGTCTAG ACACCTTCGA GGGGCTTTTG TTGTTCGGCA AGGAGCACTG TTACATTGTG 
GATGGCTTTA CGTTGTTAAA GAACCGTGAG ATTCGTGACA TTGACACCCT GCCGCCAGGA 
GCATATGAGC CAATCATTCC CAACTCGGGT GGAACATCCT CGACAACTTC GCGAGCAGTT 
AGCCACAAGC TCAGGCAGTG CTCCAAGTTT GCGTACGAGG AAATCCGGGA GGTGCACAAG 
CGTCGCTACT TGCTTCAGCC TATCGCTCTA GAAGTATTCT CTGAGGATGG GCGAAACTAT 
CTTTTGAGTT TTCCCCGCAA GGTGCGCAAC AAGGTTAACC AGCGGTTTCT GGCTCTGGCC 
ACTGCGCTGA ACGACAACGC GCAGCAGTCC GTGGCGGGTC AGAAGCGCAC GGCCAGCGTG 
GAGCAAACAG CTGGCATCTT CAGTGGACTA ATAGGCGAGA CATCGGTGAC CCAGCGATGG 
GTGCGCGGTG AGATCTCAAA CTTTCAGTAC CTTATGCATC TGAACACACT GGCCGGACGC 
AGCTACAACG ACCTGATGCA GTACCCCGTA TTTCCCTGGA TCCTTGCCGA CTACGATTCC 
GAAGAGTTGG ATCTGACAAA CCCGAAAACA TTCCGAGACT TTTCGCGCCC AATGGGCGCC 
CAAGCCGAGG AACGTCTGGA ACAGTTTCAG AAGCGCTTCA AAGAGTGGGA CGATCCGCAC 
GGGGAGACAC CGCCATACCA TTATGGCACA CATTACAGTT CTGCGATGAT CGTGTGCTCG 
TATCTGGTGC GGCTAGAGCC TTTTTCGCAG CCATTCCTCA AGTTGCAGGG CGGACACTTC 
GACCTTGCGG ACCGCATGTT TCACAGCATA AAGGAGGCGT GGCTATCTGC CTCTAAACTA 
AACATGGCCG ACGTCAAGGA ACTCATTCCG GAGTTCTTTT ACCTGCCCGA GTTTCTCAGC 
AATTTCAACA ACTTTGATCT GGGTACCAAG CAGAATGGGG AAACCCTCAA CCACGTTATC 
CTGCCGCCAT GGGCCAAACA CGATCCCAGA GAGTTCATCC GGCTGCACAG AAGTGCTTTG 
GAATGCGACT ATGTCAGCCA GCATTTGCAT TTGTGGATTG ACTTAATCTT TGGCTGTAAG 
CAACAGGGAC CAGCCGCCGT TGACGCAGTT AATGTGTTTC ATCATCTTTT CTACGAGGGA 
AACGTGGATA TCTACAACAT TGATGATCCG CTCAAAAAGA ATGCGACAAT TGGGTTCATT 
AACAATTTTG GTCAGATTCC AAAGCAGCTA TTCAAAAAGG CGCATCCTGC GAAAAAGATG 
GGCGGCTCTC GCCACTCGGC TCTAATAGAT CCTACATCTC TGATTCAGGG TAATAGCACA 
GTGCTGCAAA CCGACCGCCT GTTCTTTCAC AACTTGGACA ACCTCAAGCC GAGTCTGCAA 
CCCATCAAGG AGCTGAAAGG TCCAGTTGGT CAGATCTTGC AGCCAGACAA GACGGTGTTT 
GCTGTGGAGC AAAACAAGGT AATGATGCCG CCTTCGTATA CAAAATACAT AGCCTGGGGC 
TTTGCCGATC ATTCGCTGCG CATTGGGCTC TACGACACGG ACCGGGCATC CTTTGTGTCC 
GAGGCGTCGG CCCAGAACTC GGGAGAGATC CTGACCTGCG CCTGCCCGAA TGCCAAGATG 
ATTGTTACAG CTGGCACTAG TTCCGTGGTG ACCATTTGGA AGTTCGATGC GAACCGAAAG 
AGCCTGGCCG TTAAGCACTC GTTGCATGGA CACACCGACG CTGTGACCTG TCTAGCCGCA 
AGTGCTGCTT ACAATGTCAT CGTGTCTGGA TCGCGGGATG GCACAGCAAT CGTCTGGGAC 
ATGACACGGT TCACTTTCGT TCGTCAGTTG CGGGGTCATG CTGGTGTGGT GGCGGCCGTT 
TCCATTAATG AGCTGACCGG GGACATTGCA ACCTGCTCAG CCACCTGGCT CCACGTGTGG 
TCCATCAATG GCGATGCCTT AGCTATGGTC AACACATGTG TCGGCAGCGC GGATCGCATG 
CAGCAGATCC TGTGTGTGGC CTTCTCACAG ATCCGGGAGT GGGACCAGCA AAATGTCATC 
ATAACTGGAT CAACCGACGG TGTAGTGAGG ATGTGGTCTC TGGAGCATAC ACAGGTGCCC 
ATCGATCGCA AATTGAAGCG AGGCACGGAG GTATCTTCGC AGGACAAACC CGACACCGTC 
TCTTTGGAAG GCAAGGACAA TAAGGACGAC AAAATGAGCC TAATGAAGCA AATAAAAAGT 
CTTTCCCAGT CGGAGGAAGA GATGGAGATT GTCAAATCCG CATCGGAAAG TAGCATTTCG 
GAGGCCTCCC AACATAGTCA TGGATCGAGT AAGTCGGCTG AGGCAGGTCC TAAGGCTGAA 
GTGCAGGGTG AACGCAGGAA AAGTTCCATT TCTGGTGCAA AGTCATTGCA CGAAATGAAG 
TCTGCTACAG TGGAAGGCCA GGGCAGCAGC TACGATCCCA AATCCAATGA AGATGAACAT 
GCTATAAGAC CGAGCAAATC AGATACAAGC CTCACGGACG GTTTCGTTGT AATAGATAAT 
GACAGGCATA AAGGCGATCA GATTCTTAGA AAGGGCTTTA GATGGCAGCG TCAATTAATG 
TTCCGATCGA AGTTAACCAT GCATACGGCA TACGATCGCA AGGACAATGC TGAGCCAGCC 
AGCATAACAG CACTAACCGT GTCCAAAGAC CATAAAATTT TATATGTCGG TGATGCACGC 
GGTCGCATCT TTTCATGGAG CGTTACGGAG CAACCTGGTC GCGGAGTAGC TGACCATTGG 
CTGAAAGATG AGGGAGCAGA TCAGTGCGTC AAGTGCCACG TCAAATTTAC ACTCTATGAG 
CGAAAGCATC ATTGTCGCAA CTGTGGCCAA GTTTTCTGCA ACAAGTGCAG TCGCTTCGAG 
TCGGAAATCT CGCGTCTGCG AATCCTGAAG CCGGTCAGGG TGTGCCAGGC GTGCTACAGT 
CAGCTGCGCA CGAACTCGTT GGACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608968.2
CDS	152..10621
Translation	MNVMRKLRGAASAGSVSGSSSGTSTANNSSPGSSRTDAGAPTAANGRNAEEALMDARVQVSLTTLKKLFNEYTHPREPLSEQERDDKLYEMLPLFCKVFSSCPSNDMSEKFWDVVAFCQQVSRLMVSEIRKRASNQSTEAASIAIVKFLEVETTEETSSGWMLLATLNLLANGDVSLIQVMTAAAVPSTLVKCLYLFFDLPIVEDDEPSADGGAVSEFNAHERRTLLQKVFVQLLVKLCSYPYPAEELARMDDLTLLFSAITSPCPIHNIVWRKNAAEILTTISRNGLTDAVVSYIHSKGCMALCVDNMQRLTFGNPLEIVEMFVTVFCFLKDSSQVSQILMDDFRASQGYVFLSDFLLKFDNNRSQSLEIQAAIRNLVLMISSLCMCGFYELRPPASQFNTAFKLQNFQLPQATSRETCVRNVYAFQVLQNVFLKSTTPALCCTILDAISRVYHSENANYFILESEQTLSSFAERIHMKSPQIQEKFYDLLEFIVFQLNFVPCKELISLSLLLKHNQSTSCSILCLKTLLNILRHNAVFKDVYREVGILEIFVGCLTRYAAHVQKITKGDESVVVELENESEEELFDTLGKHVLEALTMLLGGGASNNAQLFREYGGAKCVHELVKFKHCRPQALGIVRELILSAGGDDDMLHILSLMHSVSPLQVEFKIQILNMLLGCLKDSHRTRTVFRKVGGFVYVTSVFVSLDGSMALPQPDIPQQDLILLLQIVFQTLATAMRFEPANAKFFHQEISSSSLCDTLRLLGCFGGSSALQDYTGTYEPQQSLLKYYHEIFSGDILSVSFSEDVPYPLSYVCIVFRLLYSIALDNFEAPNLSGIITLFSDPPTTLRSPSKELAPPVHPSQLNLTQPSPEPRIVHPGVVLCMLQLLPAVEYDMAPLQAVQLQVYLSEIIKSLVRSERNQQIMCDHGLAEKLLKLTRRALAEESHPLHVPMQYILERLAAQALQPTELRQFLRLGEPLSCADIDLQQPYKLGGPVPLTRIKTLVSMTTPRDFRAHGSSTLPPFVELDMSAEGFGCLYLPSLAPQATATAGGTIDANSIGGIGAGDRIFPPQTGLTYSTWFCVEKFSDPKTDPHCVRLLTLVRTIHNPREENLACLSILLSARDKAIVVSTQETLVTPRKSIGDWEPEGSDDGIARIWCPDLLHEGQWHNLVVVLNRAVLKNSSLFLYLDGVPMHTQKLHYIAQHPAAGNASLTSPTQIFGYIGTPPIWRRYSRLCWKQGVCHLIEDVLTQQTVQTIYQLGPHYMGSLQAPQLGKQSESLAPLVPEDRVLLGLNAKAVSKLTLVKIRKVYSRADNKSIAKQLNMNSHENATPIKILHNSAGHLAGAGRSLGGVVVGYLGVRVFSPHPVSAMIDTVGGCNVLLGIIAMAQDVESLYAGVKALTCVVRSNRAAQAEMDRKRCYQTLGMFFKKKKHLLNSHILHLTFGLVGTVNSGQDMSAIPNVTAFQDLLCDLEIWHNAPNGLLRSLLEHLLELVVESSDKKQNVKIMRDLQLLVKLLHIITQIQDHSTREILFSLLETLLGGQPRHTDLLLFGQYVAAKLPHAHSGGLERAVLLPSMKNPAEDQDGGVAQNIYLRNRCLSLLHGLLFTPRNTVNYVICDDISKTLGMDWLLLFMQPHVHFTTVIIAVRILVVICANESFLVRFRDATHNGGYLRFTEMVSQRKMLGLGAQQLNQRPTNGTGTVIVATPQNTIQHLPTQIAGEVRAAALNIPGFQLLEWLMNHHLDVPELYFLITALIMGQPVKVLATEHTKFDLDRVWSFLWGAPVSANTQLPKLNICPEGVCVLLAMVRGIVHGGECAPWLHSHPETIIQLLFSLYQNLTDFAPVMMTGDVVTSLVAVLFPLASRPADSEPNSGASTPTDDTGSSFALPPPETLHGAPQPKLTAHPVCNCIIDFLRVIVVDSLGLNMQGKPTPVIDLVLDAAPESAELPLQVQYQTQIIIALMDHLLAADVLVGEQAALPLVPLLQSQMQYIAPNVFYLTARIVDKLWQGCLARNPHDIFDFVIKLIVQAKRRSSSLSLEHLHHSLNRSILFLLSRPTDDSRADQMSVLEALHKIIQHRLLIFGAGNHELEFIGCLTYCLLQLTADMKIILEPATSRNTTWHVNPQTETAEPKDEDLNQLQGRNLIVGAAFRVWEELYVCKKPAIEEVFKVSLTSPPPNSKAPDLQTTREQVMELASKLWFNYVEAERKATYRAPWELHTQIQSKIQKVTGGLSRLTSRTKTKKEELVRTRSTLTREAAYESTGIHVQLIKDLLDLRAKQYQQMLQHTQRYVYQDWVQSEMELTRERGLWGPTGSCSLDKWILDTTEGPHRMRKKTMRNDVFYLHYPYRPELELADNRQLKYKVASSLDSKTYALHGPQQPRILAEAGEHHAMQQQSSLEAVQSHRLETSSSTSTPPPMVLPKLVGHGSTPCPQESVDGNAPEDDEEEEDTSMTSDNETFLRLLEEQEKISFMFRCARVQGLDTFEGLLLFGKEHCYIVDGFTLLKNREIRDIDTLPPGAYEPIIPNSGGTSSTTSRAVSHKLRQCSKFAYEEIREVHKRRYLLQPIALEVFSEDGRNYLLSFPRKVRNKVNQRFLALATALNDNAQQSVAGQKRTASVEQTAGIFSGLIGETSVTQRWVRGEISNFQYLMHLNTLAGRSYNDLMQYPVFPWILADYDSEELDLTNPKTFRDFSRPMGAQAEERLEQFQKRFKEWDDPHGETPPYHYGTHYSSAMIVCSYLVRLEPFSQPFLKLQGGHFDLADRMFHSIKEAWLSASKLNMADVKELIPEFFYLPEFLSNFNNFDLGTKQNGETLNHVILPPWAKHDPREFIRLHRSALECDYVSQHLHLWIDLIFGCKQQGPAAVDAVNVFHHLFYEGNVDIYNIDDPLKKNATIGFINNFGQIPKQLFKKAHPAKKMGGSRHSALIDPTSLIQGNSTVLQTDRLFFHNLDNLKPSLQPIKELKGPVGQILQPDKTVFAVEQNKVMMPPSYTKYIAWGFADHSLRIGLYDTDRASFVSEASAQNSGEILTCACPNAKMIVTAGTSSVVTIWKFDANRKSLAVKHSLHGHTDAVTCLAASAAYNVIVSGSRDGTAIVWDMTRFTFVRQLRGHAGVVAAVSINELTGDIATCSATWLHVWSINGDALAMVNTCVGSADRMQQILCVAFSQIREWDQQNVIITGSTDGVVRMWSLEHTQVPIDRKLKRGTEVSSQDKPDTVSLEGKDNKDDKMSLMKQIKSLSQSEEEMEIVKSASESSISEASQHSHGSSKSAEAGPKAEVQGERRKSSISGAKSLHEMKSATVEGQGSSYDPKSNEDEHAIRPSKSDTSLTDGFVVIDNDRHKGDQILRKGFRWQRQLMFRSKLTMHTAYDRKDNAEPASITALTVSKDHKILYVGDARGRIFSWSVTEQPGRGVADHWLKDEGADQCVKCHVKFTLYERKHHCRNCGQVFCNKCSRFESEISRLRILKPVRVCQACYSQLRTNSLDN

Target ORF information:

RefSeq Version	NM_135124.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster blue cheese (bchs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135124.2

ORF Insert Sequence:

ATGAATGTAA TGCGTAAGCT GCGCGGAGCG GCCAGCGCAG GCAGCGTCAG CGGCAGCAGT 
AGCGGAACCT CGACGGCGAA CAACAGTTCA CCAGGGAGCA GCAGAACGGA TGCTGGTGCA 
CCGACCGCTG CAAATGGACG GAATGCGGAG GAGGCGCTGA TGGACGCCCG CGTCCAAGTG 
AGCCTCACCA CGCTAAAGAA GCTATTCAAC GAATACACTC ATCCGCGGGA GCCGCTGAGC 
GAACAGGAGC GCGATGACAA GCTCTACGAG ATGCTGCCCC TCTTCTGCAA AGTCTTCAGC 
AGCTGTCCGT CAAACGACAT GAGCGAAAAG TTCTGGGACG TGGTCGCCTT CTGCCAGCAA 
GTGTCCCGTC TCATGGTCAG CGAAATCCGC AAGCGCGCCT CCAACCAGAG CACAGAGGCG 
GCATCCATTG CCATAGTCAA GTTTCTCGAG GTGGAAACCA CCGAGGAAAC CAGCAGCGGA 
TGGATGCTTT TGGCTACCCT CAATTTGTTG GCCAATGGAG ACGTATCTCT CATACAGGTC 
ATGACTGCTG CGGCGGTTCC CTCTACTTTA GTGAAGTGCT TGTACTTGTT CTTCGACCTG 
CCCATAGTTG AGGACGATGA GCCTTCAGCT GACGGTGGAG CAGTAAGTGA GTTTAATGCC 
CATGAAAGGC GGACGCTTCT GCAGAAGGTG TTTGTGCAGC TGCTGGTCAA GCTGTGCTCG 
TATCCGTATC CCGCTGAGGA ACTAGCTCGC ATGGACGACT TGACGCTGCT ATTCTCGGCC 
ATCACATCGC CATGTCCCAT CCACAATATT GTATGGCGCA AGAACGCAGC TGAAATCCTG 
ACCACAATAT CAAGGAATGG CCTAACCGAT GCCGTAGTTA GCTATATACA TTCCAAGGGC 
TGCATGGCAC TTTGCGTGGA CAATATGCAG CGCCTGACGT TTGGAAATCC TTTGGAGATC 
GTTGAAATGT TCGTCACCGT GTTCTGCTTT CTCAAGGACT CCAGTCAGGT ATCCCAGATT 
TTGATGGATG ATTTTCGTGC ATCTCAAGGT TACGTGTTTC TCAGTGATTT TCTACTCAAG 
TTTGACAACA ATCGTAGCCA ATCATTGGAG ATTCAAGCGG CCATTCGAAA TCTGGTACTG 
ATGATCTCTT CGTTGTGCAT GTGTGGCTTT TACGAGCTTC GACCGCCAGC CTCGCAGTTT 
AATACGGCTT TCAAACTGCA GAACTTCCAG CTCCCACAGG CCACCTCACG AGAGACATGC 
GTGCGAAATG TGTACGCCTT CCAGGTGCTG CAGAACGTGT TCCTTAAGTC AACGACGCCT 
GCGCTCTGCT GCACAATACT GGACGCCATT TCGCGGGTGT ACCACTCGGA AAATGCCAAC 
TACTTTATCC TGGAATCGGA GCAAACGCTT AGTTCGTTCG CAGAGCGGAT ACACATGAAA 
AGCCCGCAAA TACAAGAAAA ATTCTACGAT CTGCTGGAGT TCATTGTCTT CCAACTGAAC 
TTCGTGCCGT GCAAGGAGCT GATCAGCCTT TCGCTATTGC TCAAGCACAA CCAGTCGACG 
TCCTGCAGCA TTCTTTGCTT GAAGACACTG TTAAACATTC TCCGGCACAA CGCTGTATTT 
AAAGATGTCT ACCGCGAAGT GGGGATTCTG GAGATCTTTG TTGGCTGTCT AACACGCTAT 
GCAGCTCATG TGCAGAAAAT AACCAAGGGA GATGAGTCAG TGGTGGTGGA GCTGGAAAAT 
GAATCCGAAG AAGAACTGTT CGACACACTA GGAAAGCATG TTCTGGAGGC CCTCACCATG 
CTGCTGGGAG GTGGTGCCAG CAACAATGCC CAACTATTTC GTGAGTACGG TGGCGCCAAG 
TGCGTCCATG AGTTGGTGAA GTTCAAGCAC TGCCGACCGC AGGCTCTCGG AATCGTAAGG 
GAGCTAATCC TGTCCGCAGG TGGAGACGAC GACATGCTGC ACATACTCTC ACTGATGCAC 
AGCGTCAGCC CACTACAAGT GGAGTTTAAG ATTCAGATAC TAAACATGTT GCTCGGCTGC 
CTAAAGGACT CTCATCGCAC ACGGACCGTA TTCCGCAAAG TGGGCGGATT CGTTTACGTG 
ACCAGTGTAT TTGTGTCTCT GGATGGCAGC ATGGCCCTCC CTCAACCGGA CATTCCACAA 
CAGGATCTCA TTCTGCTGCT GCAAATAGTC TTCCAGACTC TAGCCACCGC TATGAGATTT 
GAACCGGCCA ACGCCAAGTT CTTTCACCAG GAGATTAGCA GCAGCTCATT GTGCGACACA 
CTTCGTCTGC TGGGATGCTT TGGTGGATCG AGTGCGTTAC AGGACTACAC GGGCACCTAC 
GAGCCGCAGC AGTCGCTCCT CAAGTATTAT CATGAGATTT TCAGCGGTGA TATTCTAAGC 
GTCAGTTTCT CTGAAGACGT TCCATACCCG CTGTCATACG TGTGCATCGT ATTTCGGCTA 
CTATACAGTA TTGCCCTAGA TAATTTTGAG GCTCCAAATC TAAGCGGCAT TATTACACTC 
TTCAGCGATC CTCCGACAAC TTTGCGATCG CCGAGTAAAG AGCTGGCGCC ACCCGTGCAT 
CCCAGCCAGC TGAATCTCAC GCAGCCCAGT CCGGAGCCTA GAATAGTGCA CCCAGGTGTG 
GTTCTTTGCA TGCTTCAGCT GCTCCCCGCC GTGGAGTACG ACATGGCTCC TTTACAGGCG 
GTGCAACTGC AAGTATATCT AAGCGAAATC ATCAAGTCCT TGGTGCGAAG CGAACGCAAC 
CAGCAGATAA TGTGTGACCA CGGTCTGGCA GAGAAACTGC TTAAGCTGAC GCGAAGAGCC 
CTGGCGGAGG AGTCGCATCC GTTGCACGTG CCAATGCAGT ACATTCTGGA GCGTCTTGCC 
GCTCAAGCAT TGCAACCTAC CGAATTGCGC CAGTTCCTGC GGCTGGGTGA GCCGCTCTCG 
TGTGCGGATA TAGATCTTCA GCAGCCATAC AAGCTGGGTG GGCCCGTTCC GCTGACTCGT 
ATCAAGACTC TGGTGTCTAT GACAACGCCC CGTGACTTCC GAGCCCATGG TTCGAGCACG 
CTCCCTCCGT TCGTGGAGCT GGACATGTCC GCCGAGGGAT TCGGTTGCTT GTACCTGCCT 
TCATTGGCGC CACAGGCAAC GGCCACCGCT GGAGGAACCA TCGATGCAAA CAGCATTGGT 
GGCATCGGGG CTGGCGATCG CATCTTTCCA CCACAAACTG GACTCACATA CTCCACCTGG 
TTCTGTGTAG AAAAGTTCTC AGATCCCAAG ACGGACCCTC ACTGTGTTCG GCTTCTTACT 
TTGGTTAGGA CCATCCACAA TCCACGAGAG GAAAACTTAG CATGTTTATC CATTTTGCTT 
TCCGCACGGG ACAAGGCGAT CGTCGTCTCG ACGCAGGAGA CGTTGGTCAC ACCCAGAAAA 
AGTATTGGTG ACTGGGAACC CGAAGGATCG GATGACGGTA TCGCTCGCAT ATGGTGTCCT 
GATCTTTTGC ACGAAGGTCA GTGGCACAAC CTGGTCGTTG TACTCAACAG GGCTGTGCTG 
AAAAATTCGA GTCTGTTCCT CTATTTGGAC GGGGTTCCAA TGCATACTCA AAAGCTGCAC 
TATATTGCCC AGCATCCCGC AGCAGGAAAC GCCAGTTTGA CGTCGCCCAC ACAGATTTTT 
GGATATATCG GTACGCCACC AATATGGCGG CGTTACTCAC GTCTCTGTTG GAAGCAGGGC 
GTTTGTCACC TTATCGAGGA TGTGCTAACG CAGCAAACAG TTCAGACAAT ATACCAACTG 
GGGCCTCACT ACATGGGCTC TTTGCAAGCT CCGCAACTTG GCAAGCAGTC GGAGTCATTG 
GCGCCTTTGG TGCCAGAGGA TCGCGTCCTC CTGGGTCTTA ATGCTAAAGC GGTGTCCAAG 
CTGACACTAG TCAAAATCCG CAAAGTTTAC AGCCGAGCGG ACAACAAAAG CATTGCCAAG 
CAGCTAAACA TGAACTCGCA CGAGAATGCT ACGCCCATAA AAATTCTTCA CAACTCTGCA 
GGGCATCTTG CAGGAGCTGG ACGCTCCCTT GGCGGCGTGG TCGTCGGTTA TCTGGGCGTC 
AGAGTATTCA GTCCACATCC CGTCTCAGCC ATGATCGACA CAGTTGGTGG CTGCAACGTG 
CTCCTCGGTA TCATCGCCAT GGCTCAAGAC GTGGAATCCC TATACGCAGG TGTCAAAGCT 
TTGACCTGCG TGGTGCGAAG CAATCGCGCC GCTCAAGCCG AGATGGACAG AAAACGATGC 
TACCAGACAT TGGGCATGTT CTTTAAAAAG AAAAAACATC TGCTAAACTC GCACATCTTG 
CACCTGACCT TTGGTCTAGT AGGCACGGTT AACAGCGGAC AAGACATGTC GGCTATTCCA 
AATGTGACAG CCTTCCAAGA TCTTTTGTGT GATCTTGAGA TTTGGCATAA TGCACCAAAC 
GGACTTCTGC GCTCCCTGCT AGAACATCTA TTGGAGCTAG TGGTCGAGTC CAGTGACAAG 
AAGCAGAACG TGAAGATCAT GCGCGACCTC CAGCTTCTAG TGAAATTGCT GCACATCATT 
ACCCAAATCC AAGACCACTC CACACGAGAA ATTCTCTTCA GTCTGCTGGA GACTCTACTG 
GGCGGTCAGC CGAGGCACAC CGATCTACTG CTTTTCGGTC AGTATGTGGC GGCCAAGTTG 
CCACATGCCC ATTCTGGAGG CTTGGAACGC GCTGTGCTGC TGCCCAGCAT GAAAAATCCT 
GCCGAGGATC AAGACGGTGG CGTGGCCCAG AACATATACC TACGTAATCG CTGTCTATCG 
CTGCTCCACG GACTGCTATT CACACCGCGA AACACCGTGA ACTATGTAAT CTGCGACGAC 
ATTTCCAAAA CATTGGGCAT GGATTGGTTG CTACTCTTCA TGCAACCGCA CGTCCACTTC 
ACCACTGTAA TCATTGCAGT GCGCATCCTG GTCGTAATCT GTGCTAACGA AAGTTTTCTT 
GTGCGATTCC GGGATGCCAC CCACAACGGC GGTTATCTTC GGTTTACCGA GATGGTCTCT 
CAGCGAAAGA TGCTGGGACT CGGTGCCCAG CAGCTTAACC AGCGACCAAC GAATGGCACT 
GGTACAGTCA TTGTGGCCAC CCCACAGAAT ACCATACAAC ATCTGCCCAC GCAGATCGCA 
GGAGAGGTGC GAGCAGCGGC CTTAAACATA CCAGGTTTCC AGCTTCTTGA ATGGCTAATG 
AATCATCACC TGGACGTTCC GGAGTTGTAC TTCCTAATCA CCGCCCTGAT TATGGGACAA 
CCTGTAAAGG TGTTGGCTAC TGAGCACACA AAGTTCGATC TTGACCGCGT CTGGTCGTTC 
CTCTGGGGAG CTCCTGTGTC AGCCAACACA CAGCTACCAA AGCTCAACAT TTGTCCCGAG 
GGCGTATGCG TACTCCTGGC AATGGTGCGG GGAATCGTTC ATGGTGGAGA GTGCGCTCCA 
TGGCTGCACA GCCACCCGGA AACAATTATC CAGCTCCTGT TTAGCTTGTA CCAAAACCTC 
ACCGACTTTG CGCCTGTGAT GATGACGGGC GATGTGGTCA CCTCGCTAGT AGCTGTGCTA 
TTTCCGTTGG CATCTCGTCC TGCTGACTCT GAGCCAAACA GTGGAGCATC GACACCCACA 
GACGATACGG GAAGCAGCTT TGCTTTGCCA CCGCCGGAAA CACTTCATGG AGCTCCGCAG 
CCCAAGCTAA CAGCTCATCC GGTGTGTAAT TGCATTATCG ACTTCCTGCG CGTTATTGTC 
GTGGACTCTC TCGGCCTCAA CATGCAGGGT AAACCTACTC CGGTAATCGA TCTCGTGCTG 
GACGCAGCAC CAGAATCGGC TGAACTACCC CTCCAGGTGC AGTATCAGAC CCAGATAATT 
ATCGCCCTAA TGGATCACCT ACTGGCCGCC GACGTGCTTG TAGGCGAACA GGCAGCACTC 
CCGCTGGTGC CTCTCTTGCA GAGCCAAATG CAATACATCG CCCCAAACGT GTTTTACCTG 
ACCGCCCGCA TCGTAGATAA ACTATGGCAA GGTTGCCTCG CCCGCAATCC ACACGACATT 
TTCGATTTTG TCATAAAACT GATCGTACAG GCGAAGCGTC GGTCGTCCTC GTTGTCATTG 
GAGCATCTTC ATCACTCGCT GAACCGCAGC ATCCTCTTCC TGCTTTCCCG ACCCACCGAT 
GACTCGCGAG CTGACCAGAT GAGCGTGCTG GAGGCACTGC ACAAGATCAT TCAGCACCGC 
CTGCTTATTT TCGGAGCGGG CAACCACGAG TTGGAATTCA TTGGCTGCCT CACATACTGT 
TTGCTGCAGT TGACTGCCGA TATGAAAATT ATATTGGAGC CGGCAACCAG CCGAAACACT 
ACATGGCACG TAAATCCGCA GACGGAGACG GCAGAGCCCA AGGATGAAGA TCTCAACCAG 
TTACAGGGTC GGAATCTGAT CGTTGGCGCT GCTTTTCGAG TTTGGGAGGA GCTATACGTA 
TGCAAGAAGC CTGCCATTGA GGAGGTATTT AAAGTCTCCC TAACATCCCC ACCGCCGAAC 
TCCAAAGCAC CTGATCTGCA GACCACGCGA GAGCAGGTCA TGGAGCTGGC CTCTAAACTA 
TGGTTCAATT ATGTGGAGGC AGAACGTAAG GCCACATATC GTGCGCCCTG GGAGCTGCAC 
ACCCAGATCC AGTCCAAAAT CCAGAAGGTT ACTGGGGGAC TGTCCCGTTT GACTAGCAGA 
ACAAAAACCA AAAAGGAGGA GCTGGTACGA ACCAGGTCGA CCTTAACTCG CGAGGCAGCC 
TATGAGTCCA CCGGCATCCA TGTGCAGCTG ATAAAGGATC TTTTGGATTT GCGTGCGAAG 
CAGTACCAGC AGATGTTGCA GCATACTCAG CGCTACGTCT ATCAGGACTG GGTGCAGTCG 
GAGATGGAGC TTACCCGGGA GCGCGGTCTG TGGGGTCCCA CTGGCAGCTG CTCATTGGAT 
AAGTGGATTT TAGACACCAC CGAAGGACCC CACCGTATGC GCAAGAAAAC GATGCGTAAT 
GATGTCTTCT ACCTGCACTA TCCATACAGA CCGGAGTTGG AGCTGGCGGA CAATCGGCAG 
CTGAAGTACA AGGTGGCCTC CAGTCTAGAT AGCAAGACGT ACGCACTGCA CGGACCGCAG 
CAGCCACGCA TACTGGCCGA GGCGGGGGAG CACCATGCGA TGCAACAGCA GAGTTCTCTG 
GAAGCGGTGC AATCGCATCG GTTGGAGACC AGCAGTTCGA CATCAACGCC ACCACCCATG 
GTTTTGCCCA AGCTTGTGGG ACACGGATCA ACACCCTGTC CACAGGAGTC GGTGGACGGC 
AATGCTCCAG AGGATGACGA AGAAGAAGAG GATACTTCGA TGACCAGCGA TAATGAGACG 
TTTTTGAGGC TACTAGAGGA GCAGGAGAAG ATTAGTTTTA TGTTCCGTTG TGCAAGAGTC 
CAAGGTCTAG ACACCTTCGA GGGGCTTTTG TTGTTCGGCA AGGAGCACTG TTACATTGTG 
GATGGCTTTA CGTTGTTAAA GAACCGTGAG ATTCGTGACA TTGACACCCT GCCGCCAGGA 
GCATATGAGC CAATCATTCC CAACTCGGGT GGAACATCCT CGACAACTTC GCGAGCAGTT 
AGCCACAAGC TCAGGCAGTG CTCCAAGTTT GCGTACGAGG AAATCCGGGA GGTGCACAAG 
CGTCGCTACT TGCTTCAGCC TATCGCTCTA GAAGTATTCT CTGAGGATGG GCGAAACTAT 
CTTTTGAGTT TTCCCCGCAA GGTGCGCAAC AAGGTTAACC AGCGGTTTCT GGCTCTGGCC 
ACTGCGCTGA ACGACAACGC GCAGCAGTCC GTGGCGGGTC AGAAGCGCAC GGCCAGCGTG 
GAGCAAACAG CTGGCATCTT CAGTGGACTA ATAGGCGAGA CATCGGTGAC CCAGCGATGG 
GTGCGCGGTG AGATCTCAAA CTTTCAGTAC CTTATGCATC TGAACACACT GGCCGGACGC 
AGCTACAACG ACCTGATGCA GTACCCCGTA TTTCCCTGGA TCCTTGCCGA CTACGATTCC 
GAAGAGTTGG ATCTGACAAA CCCGAAAACA TTCCGAGACT TTTCGCGCCC AATGGGCGCC 
CAAGCCGAGG AACGTCTGGA ACAGTTTCAG AAGCGCTTCA AAGAGTGGGA CGATCCGCAC 
GGGGAGACAC CGCCATACCA TTATGGCACA CATTACAGTT CTGCGATGAT CGTGTGCTCG 
TATCTGGTGC GGCTAGAGCC TTTTTCGCAG CCATTCCTCA AGTTGCAGGG CGGACACTTC 
GACCTTGCGG ACCGCATGTT TCACAGCATA AAGGAGGCGT GGCTATCTGC CTCTAAACTA 
AACATGGCCG ACGTCAAGGA ACTCATTCCG GAGTTCTTTT ACCTGCCCGA GTTTCTCAGC 
AATTTCAACA ACTTTGATCT GGGTACCAAG CAGAATGGGG AAACCCTCAA CCACGTTATC 
CTGCCGCCAT GGGCCAAACA CGATCCCAGA GAGTTCATCC GGCTGCACAG AAGTGCTTTG 
GAATGCGACT ATGTCAGCCA GCATTTGCAT TTGTGGATTG ACTTAATCTT TGGCTGTAAG 
CAACAGGGAC CAGCCGCCGT TGACGCAGTT AATGTGTTTC ATCATCTTTT CTACGAGGGA 
AACGTGGATA TCTACAACAT TGATGATCCG CTCAAAAAGA ATGCGACAAT TGGGTTCATT 
AACAATTTTG GTCAGATTCC AAAGCAGCTA TTCAAAAAGG CGCATCCTGC GAAAAAGATG 
GGCGGCTCTC GCCACTCGGC TCTAATAGAT CCTACATCTC TGATTCAGGG TAATAGCACA 
GTGCTGCAAA CCGACCGCCT GTTCTTTCAC AACTTGGACA ACCTCAAGCC GAGTCTGCAA 
CCCATCAAGG AGCTGAAAGG TCCAGTTGGT CAGATCTTGC AGCCAGACAA GACGGTGTTT 
GCTGTGGAGC AAAACAAGGT AATGATGCCG CCTTCGTATA CAAAATACAT AGCCTGGGGC 
TTTGCCGATC ATTCGCTGCG CATTGGGCTC TACGACACGG ACCGGGCATC CTTTGTGTCC 
GAGGCGTCGG CCCAGAACTC GGGAGAGATC CTGACCTGCG CCTGCCCGAA TGCCAAGATG 
ATTGTTACAG CTGGCACTAG TTCCGTGGTG ACCATTTGGA AGTTCGATGC GAACCGAAAG 
AGCCTGGCCG TTAAGCACTC GTTGCATGGA CACACCGACG CTGTGACCTG TCTAGCCGCA 
AGTGCTGCTT ACAATGTCAT CGTGTCTGGA TCGCGGGATG GCACAGCAAT CGTCTGGGAC 
ATGACACGGT TCACTTTCGT TCGTCAGTTG CGGGGTCATG CTGGTGTGGT GGCGGCCGTT 
TCCATTAATG AGCTGACCGG GGACATTGCA ACCTGCTCAG CCACCTGGCT CCACGTGTGG 
TCCATCAATG GCGATGCCTT AGCTATGGTC AACACATGTG TCGGCAGCGC GGATCGCATG 
CAGCAGATCC TGTGTGTGGC CTTCTCACAG ATCCGGGAGT GGGACCAGCA AAATGTCATC 
ATAACTGGAT CAACCGACGG TGTAGTGAGG ATGTGGTCTC TGGAGCATAC ACAGGTGCCC 
ATCGATCGCA AATTGAAGCG AGGCACGGAG GTATCTTCGC AGGACAAACC CGACACCGTC 
TCTTTGGAAG GCAAGGACAA TAAGGACGAC AAAATGAGCC TAATGAAGCA AATAAAAAGT 
CTTTCCCAGT CGGAGGAAGA GATGGAGATT GTCAAATCCG CATCGGAAAG TAGCATTTCG 
GAGGCCTCCC AACATAGTCA TGGATCGAGT AAGTCGGCTG AGGCAGGTCC TAAGGCTGAA 
GTGCAGGGTG AACGCAGGAA AAGTTCCATT TCTGGTGCAA AGTCATTGCA CGAAATGAAG 
TCTGCTACAG TGGAAGGCCA GGGCAGCAGC TACGATCCCA AATCCAATGA AGATGAACAT 
GCTATAAGAC CGAGCAAATC AGATACAAGC CTCACGGACG GTTTCGTTGT AATAGATAAT 
GACAGGCATA AAGGCGATCA GATTCTTAGA AAGGGCTTTA GATGGCAGCG TCAATTAATG 
TTCCGATCGA AGTTAACCAT GCATACGGCA TACGATCGCA AGGACAATGC TGAGCCAGCC 
AGCATAACAG CACTAACCGT GTCCAAAGAC CATAAAATTT TATATGTCGG TGATGCACGC 
GGTCGCATCT TTTCATGGAG CGTTACGGAG CAACCTGGTC GCGGAGTAGC TGACCATTGG 
CTGAAAGATG AGGGAGCAGA TCAGTGCGTC AAGTGCCACG TCAAATTTAC ACTCTATGAG 
CGAAAGCATC ATTGTCGCAA CTGTGGCCAA GTTTTCTGCA ACAAGTGCAG TCGCTTCGAG 
TCGGAAATCT CGCGTCTGCG AATCCTGAAG CCGGTCAGGG TGTGCCAGGC GTGCTACAGT 
CAGCTGCGCA CGAACTCGTT GGACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bchs cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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