Lam cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lam
Entrez Gene ID	33782
Full Name	Lamin
Synonyms	2459,74/76,CG6944,D5,DM[[O]],D[[m0]],Dm,Dm(0),Dm0,Dm1,Dm2,DmLamin,DmO,Dm[[0]],Dm[[1]],Dm[[2]],Dm[[mit]],Dm[[o]],Dmel\CG6944,Dmo,LAM,Lam Dm[[0]],Lam(Dm0),LamDm,LamDm0,LamDm[[0]],LamDm[[o]],Lam[[Dm0]],Lamin B,Lamin Dm[[0]],jf27,l(2)04643,l(2)25Ec,l(2)gdh-7,l(2)gdh7,l(2)jf27,lam,lamDm0,lamDm[[0]],lamin,lamin B,lamin Dm0,lamin Dm[[0]],lamin-B,misg,nlam
General protein information	Preferred Names Lamin Names CG6944 gene product from transcript CG6944-RD B-type lamin CG6944-PA CG6944-PB CG6944-PC CG6944-PD Lam-PA Lam-PB Lam-PC Lam-PD Lamin-B LaminDm LaminDm0 alpha-Lamin intermediate filament-like lamina lamin lamin A lamin B lamin D lamin D[[0]] lamin D[[m0]] lamin D[[m]]0 lamin D[[o]] lamin Dm lamin Dm0 lamin Dm[[0]] lamin Dmo lethal(2)25Ec misguided misrouted n-lamin nuclear lamin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 25E6-25E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_057268.5	NP_476616.1	lamin, isoform A
NM_001258963.2	NP_001245892.1	lamin, isoform B
NM_001258964.2	NP_001245893.1	lamin, isoform C
NM_001298700.1	NP_001285629.1	lamin, isoform D

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	ifd-1	NP_001024830.1
Caenorhabditis elegans (roundworm)	ifa-3	NP_510649.3
Caenorhabditis elegans (roundworm)	ifa-4	NP_508836.4
Pan troglodytes (chimpanzee)	LMNB2	XP_524044.4
	LMNB2	XP_002801064.1
Gallus gallus (chicken)	LMNB2	NP_990616.1
Drosophila melanogaster (fruit fly)	LamC	NP_523742.2
Caenorhabditis elegans (roundworm)	ifd-2	NP_508160.1
Xenopus tropicalis (tropical clawed frog)	lmnb2	XP_002940085.1
Homo sapiens (human)	LMNB2	NP_116126.3
Bos taurus (cattle)	LMNB2	NP_001263282.1
Mus musculus (house mouse)	Lmnb2	NP_034852.2
Rattus norvegicus (Norway rat)	Lmnb2	XP_216850.6
Danio rerio (zebrafish)	lmnb2	NP_571077.2
Drosophila melanogaster (fruit fly)	Lam	NP_001245892.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011938	XP_320593.4
Caenorhabditis elegans (roundworm)	ifa-1	NP_001076764.1
Caenorhabditis elegans (roundworm)	ifb-1	NP_495136.1
Caenorhabditis elegans (roundworm)	F10C1.8	NP_495132.2
Caenorhabditis elegans (roundworm)	ifp-1	NP_509628.1
Caenorhabditis elegans (roundworm)	mua-6	NP_510648.1
Caenorhabditis elegans (roundworm)	ifb-2	NP_495134.1
Canis lupus familiaris (dog)	LMNB2	XP_542188.3
Caenorhabditis elegans (roundworm)	ifc-1	NP_503783.1
Caenorhabditis elegans (roundworm)	ifc-2	NP_001256975.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

nuclear Wash interacts with B-type Lamin (Lamin Dm0), and, like Lamin, Wash associates with constitutive heterochromatin
Title: Wash interacts with lamin and affects global nuclear organization.

The loss of lamin-B leads to systemic inflammation and aging.
Title: Lamin-B in systemic inflammation, tissue homeostasis, and aging.

of single phosphorylation sites of Drosophila melanogaster lamin Dm and lamin C
Title: The different function of single phosphorylation sites of Drosophila melanogaster lamin Dm and lamin C.

Rab5 associated in vivo with nuclear Lamin and mushroom body defect (Mud), the Drosophila counterpart of nuclear mitotic apparatus protein (NuMA).
Title: Rab5 GTPase controls chromosome alignment through Lamin disassembly and relocation of the NuMA-like protein Mud to the poles during mitosis.

Kugelkern and lamin Dm0 affect nuclear shape without requiring filament formation or the presence of a classical nuclear lamina.
Title: Farnesylated nuclear proteins Kugelkern and lamin Dm0 affect nuclear morphology by directly interacting with the nuclear membrane.

The following Lam gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lam cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13807	NM_057268.5 Latest version!	Drosophila melanogaster lamin, transcript variant A (Lam), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13807	NM_001258964.2 Latest version!	Drosophila melanogaster lamin, transcript variant C (Lam), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13807	NM_001298700.1 Latest version!	Drosophila melanogaster lamin, transcript variant D (Lam), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13807	NM_001258963.2 Latest version!	Drosophila melanogaster lamin, transcript variant B (Lam), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lam ( NM_057268.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476616.1 Drosophila melanogaster lamin, transcript variant A (Lam), mRNA.

Price & Availability↑

CloneID	OFa13807
Clone ID Related Accession (Same CDS sequence)	NM_057268.5 , NM_001258963.2 , NM_001258964.2 , NM_001298700.1
Accession Version	NM_057268.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lamin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057268.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAGCA AATCCCGACG TGCTGGCACC GCCACGCCGC AGCCCGGCAA CACCTCCACC 
CCCCGGCCGC CATCGGCGGG TCCGCAGCCG CCGCCGCCGT CCACTCACTC GCAGACGGCC 
TCCAGCCCCC TCAGCCCCAC CCGGCACTCG CGCGTGGCCG AGAAGGTGGA GCTGCAGAAC 
CTGAACGATC GCCTGGCCAC CTACATTGAC CGGGTGCGCA ACCTGGAGAC GGAGAACTCC 
CGCCTCACCA TCGAGGTGCA GACCACCAGG GACACGGTCA CACGCGAGAC CACCAACATC 
AAGAACATCT TCGAGGCCGA GCTGCTGGAG ACGCGCCGTC TGCTCGATGA CACAGCTAGG 
GATCGCGCTC GTGCCGAGAT CGATATCAAG CGTCTCTGGG AGGAGAACGA GGAGCTCAAG 
AACAAGCTGG ACAAGAAGAC CAAGGAGTGC ACCACTGCTG AGGGCAATGT CCGCATGTAC 
GAGTCGCGCG CCAACGAGCT GAACAACAAA TACAACCAGG CCAACGCCGA TCGGAAGAAG 
CTTAACGAAG ACCTGAATGA GGCGCTAAAG GAGCTGGAGA GACTGCGTAA GCAGTTCGAG 
GAAACGCGGA AGAACCTGGA ACAGGAGACA CTGTCGCGCG TTGACCTGGA GAACACCATT 
CAGAGTCTGC GCGAGGAGCT CTCGTTCAAG GATCAGATCC ATTCGCAGGA GATCAATGAG 
TCGCGCCGCA TCAAACAGAC AGAGTATAGC GAGATCGACG GTCGCCTCAG CTCCGAGTAC 
GATGCCAAGT TGAAGCAGTC GCTGCAGGAG CTGCGCGCCC AGTACGAGGA GCAGATGCAG 
ATTAATCGCG ATGAAATCCA GTCCCTCTAC GAGGACAAGA TCCAACGACT GCAAGAGGCC 
GCCGCACGCA CATCCAATTC CACGCACAAG TCCATCGAGG AGCTGCGCTC CACTCGTGTG 
CGTATCGATG CGCTCAACGC CAATATCAAC GAACTGGAGC AAGCCAATGC CGACCTCAAT 
GCGCGGATCC GTGATCTGGA GCGCCAGCTG GACAACGATC GCGAACGCCA CGGTCAAGAG 
ATAGACCTTC TCGAGAAGGA GCTCATTCGG CTGCGCGAAG AGATGACGCA ACAGCTCAAG 
GAGTACCAGG ACCTTATGGA CATCAAGGTC TCCCTGGATT TGGAAATCGC CGCATACGAC 
AAGCTGCTGG TGGGCGAGGA GGCTCGTTTG AACATCACCC CAGCCACCAA CACGGCCACA 
GTGCAGTCCT TTAGCCAGTC GCTGCGCAAC TCCACGCGAG CCACGCCATC GCGTCGCACT 
CCCTCTGCTG CCGTGAAGCG CAAACGCGCC GTGGTCGACG AGTCGGAGGA TCACAGCGTC 
GCCGATTACT ATGTGTCCGC CAGTGCCAAG GGCAACGTGG AGATCAAGGA GATCGATCCC 
GAGGGCAAGT TCGTAAGGCT GTTCAACAAG GGCAGCGAGG AGGTGGCCAT CGGTGGCTGG 
CAGCTGCAGC GGCTAATCAA CGAGAAAGGT CCTTCGACCA CTTACAAGTT CCATCGATCG 
GTGAGGATCG AGCCAAATGG CGTGATCACC GTTTGGTCGG CGGACACCAA GGCCTCGCAC 
GAGCCGCCAT CGAGCCTTGT GATGAAGTCA CAGAAGTGGG TCTCCGCCGA CAACACTAGG 
ACGATTTTGC TGAACTCCGA GGGCGAGGCC GTGGCCAATC TGGATCGCAT CAAGCGCATT 
GTGTCCCAAC ACACATCCTC CTCCCGGCTG AGTCGTCGTC GCAGCGTGAC CGCCGTGGAC 
GGCAATGAGC AGCTCTACCA CCAGCAGGGC GATCCTCAGC AGTCAAACGA GAAGTGCGCC 
ATTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476616.1
CDS	127..1995
Translation	MSSKSRRAGTATPQPGNTSTPRPPSAGPQPPPPSTHSQTASSPLSPTRHSRVAEKVELQNLNDRLATYIDRVRNLETENSRLTIEVQTTRDTVTRETTNIKNIFEAELLETRRLLDDTARDRARAEIDIKRLWEENEELKNKLDKKTKECTTAEGNVRMYESRANELNNKYNQANADRKKLNEDLNEALKELERLRKQFEETRKNLEQETLSRVDLENTIQSLREELSFKDQIHSQEINESRRIKQTEYSEIDGRLSSEYDAKLKQSLQELRAQYEEQMQINRDEIQSLYEDKIQRLQEAAARTSNSTHKSIEELRSTRVRIDALNANINELEQANADLNARIRDLERQLDNDRERHGQEIDLLEKELIRLREEMTQQLKEYQDLMDIKVSLDLEIAAYDKLLVGEEARLNITPATNTATVQSFSQSLRNSTRATPSRRTPSAAVKRKRAVVDESEDHSVADYYVSASAKGNVEIKEIDPEGKFVRLFNKGSEEVAIGGWQLQRLINEKGPSTTYKFHRSVRIEPNGVITVWSADTKASHEPPSSLVMKSQKWVSADNTRTILLNSEGEAVANLDRIKRIVSQHTSSSRLSRRRSVTAVDGNEQLYHQQGDPQQSNEKCAIM

Target ORF information:

RefSeq Version	NM_057268.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lamin, transcript variant A (Lam), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057268.5

ORF Insert Sequence:

ATGTCGAGCA AATCCCGACG TGCTGGCACC GCCACGCCGC AGCCCGGCAA CACCTCCACC 
CCCCGGCCGC CATCGGCGGG TCCGCAGCCG CCGCCGCCGT CCACTCACTC GCAGACGGCC 
TCCAGCCCCC TCAGCCCCAC CCGGCACTCG CGCGTGGCCG AGAAGGTGGA GCTGCAGAAC 
CTGAACGATC GCCTGGCCAC CTACATTGAC CGGGTGCGCA ACCTGGAGAC GGAGAACTCC 
CGCCTCACCA TCGAGGTGCA GACCACCAGG GACACGGTCA CACGCGAGAC CACCAACATC 
AAGAACATCT TCGAGGCCGA GCTGCTGGAG ACGCGCCGTC TGCTCGATGA CACAGCTAGG 
GATCGCGCTC GTGCCGAGAT CGATATCAAG CGTCTCTGGG AGGAGAACGA GGAGCTCAAG 
AACAAGCTGG ACAAGAAGAC CAAGGAGTGC ACCACTGCTG AGGGCAATGT CCGCATGTAC 
GAGTCGCGCG CCAACGAGCT GAACAACAAA TACAACCAGG CCAACGCCGA TCGGAAGAAG 
CTTAACGAAG ACCTGAATGA GGCGCTAAAG GAGCTGGAGA GACTGCGTAA GCAGTTCGAG 
GAAACGCGGA AGAACCTGGA ACAGGAGACA CTGTCGCGCG TTGACCTGGA GAACACCATT 
CAGAGTCTGC GCGAGGAGCT CTCGTTCAAG GATCAGATCC ATTCGCAGGA GATCAATGAG 
TCGCGCCGCA TCAAACAGAC AGAGTATAGC GAGATCGACG GTCGCCTCAG CTCCGAGTAC 
GATGCCAAGT TGAAGCAGTC GCTGCAGGAG CTGCGCGCCC AGTACGAGGA GCAGATGCAG 
ATTAATCGCG ATGAAATCCA GTCCCTCTAC GAGGACAAGA TCCAACGACT GCAAGAGGCC 
GCCGCACGCA CATCCAATTC CACGCACAAG TCCATCGAGG AGCTGCGCTC CACTCGTGTG 
CGTATCGATG CGCTCAACGC CAATATCAAC GAACTGGAGC AAGCCAATGC CGACCTCAAT 
GCGCGGATCC GTGATCTGGA GCGCCAGCTG GACAACGATC GCGAACGCCA CGGTCAAGAG 
ATAGACCTTC TCGAGAAGGA GCTCATTCGG CTGCGCGAAG AGATGACGCA ACAGCTCAAG 
GAGTACCAGG ACCTTATGGA CATCAAGGTC TCCCTGGATT TGGAAATCGC CGCATACGAC 
AAGCTGCTGG TGGGCGAGGA GGCTCGTTTG AACATCACCC CAGCCACCAA CACGGCCACA 
GTGCAGTCCT TTAGCCAGTC GCTGCGCAAC TCCACGCGAG CCACGCCATC GCGTCGCACT 
CCCTCTGCTG CCGTGAAGCG CAAACGCGCC GTGGTCGACG AGTCGGAGGA TCACAGCGTC 
GCCGATTACT ATGTGTCCGC CAGTGCCAAG GGCAACGTGG AGATCAAGGA GATCGATCCC 
GAGGGCAAGT TCGTAAGGCT GTTCAACAAG GGCAGCGAGG AGGTGGCCAT CGGTGGCTGG 
CAGCTGCAGC GGCTAATCAA CGAGAAAGGT CCTTCGACCA CTTACAAGTT CCATCGATCG 
GTGAGGATCG AGCCAAATGG CGTGATCACC GTTTGGTCGG CGGACACCAA GGCCTCGCAC 
GAGCCGCCAT CGAGCCTTGT GATGAAGTCA CAGAAGTGGG TCTCCGCCGA CAACACTAGG 
ACGATTTTGC TGAACTCCGA GGGCGAGGCC GTGGCCAATC TGGATCGCAT CAAGCGCATT 
GTGTCCCAAC ACACATCCTC CTCCCGGCTG AGTCGTCGTC GCAGCGTGAC CGCCGTGGAC 
GGCAATGAGC AGCTCTACCA CCAGCAGGGC GATCCTCAGC AGTCAAACGA GAAGTGCGCC 
ATTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lam ( NM_001258963.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245892.1 Drosophila melanogaster lamin, transcript variant B (Lam), mRNA.

Price & Availability↑

CloneID	OFa13807
Clone ID Related Accession (Same CDS sequence)	NM_057268.5 , NM_001258963.2 , NM_001258964.2 , NM_001298700.1
Accession Version	NM_001258963.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lamin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001258963.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAGCA AATCCCGACG TGCTGGCACC GCCACGCCGC AGCCCGGCAA CACCTCCACC 
CCCCGGCCGC CATCGGCGGG TCCGCAGCCG CCGCCGCCGT CCACTCACTC GCAGACGGCC 
TCCAGCCCCC TCAGCCCCAC CCGGCACTCG CGCGTGGCCG AGAAGGTGGA GCTGCAGAAC 
CTGAACGATC GCCTGGCCAC CTACATTGAC CGGGTGCGCA ACCTGGAGAC GGAGAACTCC 
CGCCTCACCA TCGAGGTGCA GACCACCAGG GACACGGTCA CACGCGAGAC CACCAACATC 
AAGAACATCT TCGAGGCCGA GCTGCTGGAG ACGCGCCGTC TGCTCGATGA CACAGCTAGG 
GATCGCGCTC GTGCCGAGAT CGATATCAAG CGTCTCTGGG AGGAGAACGA GGAGCTCAAG 
AACAAGCTGG ACAAGAAGAC CAAGGAGTGC ACCACTGCTG AGGGCAATGT CCGCATGTAC 
GAGTCGCGCG CCAACGAGCT GAACAACAAA TACAACCAGG CCAACGCCGA TCGGAAGAAG 
CTTAACGAAG ACCTGAATGA GGCGCTAAAG GAGCTGGAGA GACTGCGTAA GCAGTTCGAG 
GAAACGCGGA AGAACCTGGA ACAGGAGACA CTGTCGCGCG TTGACCTGGA GAACACCATT 
CAGAGTCTGC GCGAGGAGCT CTCGTTCAAG GATCAGATCC ATTCGCAGGA GATCAATGAG 
TCGCGCCGCA TCAAACAGAC AGAGTATAGC GAGATCGACG GTCGCCTCAG CTCCGAGTAC 
GATGCCAAGT TGAAGCAGTC GCTGCAGGAG CTGCGCGCCC AGTACGAGGA GCAGATGCAG 
ATTAATCGCG ATGAAATCCA GTCCCTCTAC GAGGACAAGA TCCAACGACT GCAAGAGGCC 
GCCGCACGCA CATCCAATTC CACGCACAAG TCCATCGAGG AGCTGCGCTC CACTCGTGTG 
CGTATCGATG CGCTCAACGC CAATATCAAC GAACTGGAGC AAGCCAATGC CGACCTCAAT 
GCGCGGATCC GTGATCTGGA GCGCCAGCTG GACAACGATC GCGAACGCCA CGGTCAAGAG 
ATAGACCTTC TCGAGAAGGA GCTCATTCGG CTGCGCGAAG AGATGACGCA ACAGCTCAAG 
GAGTACCAGG ACCTTATGGA CATCAAGGTC TCCCTGGATT TGGAAATCGC CGCATACGAC 
AAGCTGCTGG TGGGCGAGGA GGCTCGTTTG AACATCACCC CAGCCACCAA CACGGCCACA 
GTGCAGTCCT TTAGCCAGTC GCTGCGCAAC TCCACGCGAG CCACGCCATC GCGTCGCACT 
CCCTCTGCTG CCGTGAAGCG CAAACGCGCC GTGGTCGACG AGTCGGAGGA TCACAGCGTC 
GCCGATTACT ATGTGTCCGC CAGTGCCAAG GGCAACGTGG AGATCAAGGA GATCGATCCC 
GAGGGCAAGT TCGTAAGGCT GTTCAACAAG GGCAGCGAGG AGGTGGCCAT CGGTGGCTGG 
CAGCTGCAGC GGCTAATCAA CGAGAAAGGT CCTTCGACCA CTTACAAGTT CCATCGATCG 
GTGAGGATCG AGCCAAATGG CGTGATCACC GTTTGGTCGG CGGACACCAA GGCCTCGCAC 
GAGCCGCCAT CGAGCCTTGT GATGAAGTCA CAGAAGTGGG TCTCCGCCGA CAACACTAGG 
ACGATTTTGC TGAACTCCGA GGGCGAGGCC GTGGCCAATC TGGATCGCAT CAAGCGCATT 
GTGTCCCAAC ACACATCCTC CTCCCGGCTG AGTCGTCGTC GCAGCGTGAC CGCCGTGGAC 
GGCAATGAGC AGCTCTACCA CCAGCAGGGC GATCCTCAGC AGTCAAACGA GAAGTGCGCC 
ATTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245892.1
CDS	233..2101
Translation	MSSKSRRAGTATPQPGNTSTPRPPSAGPQPPPPSTHSQTASSPLSPTRHSRVAEKVELQNLNDRLATYIDRVRNLETENSRLTIEVQTTRDTVTRETTNIKNIFEAELLETRRLLDDTARDRARAEIDIKRLWEENEELKNKLDKKTKECTTAEGNVRMYESRANELNNKYNQANADRKKLNEDLNEALKELERLRKQFEETRKNLEQETLSRVDLENTIQSLREELSFKDQIHSQEINESRRIKQTEYSEIDGRLSSEYDAKLKQSLQELRAQYEEQMQINRDEIQSLYEDKIQRLQEAAARTSNSTHKSIEELRSTRVRIDALNANINELEQANADLNARIRDLERQLDNDRERHGQEIDLLEKELIRLREEMTQQLKEYQDLMDIKVSLDLEIAAYDKLLVGEEARLNITPATNTATVQSFSQSLRNSTRATPSRRTPSAAVKRKRAVVDESEDHSVADYYVSASAKGNVEIKEIDPEGKFVRLFNKGSEEVAIGGWQLQRLINEKGPSTTYKFHRSVRIEPNGVITVWSADTKASHEPPSSLVMKSQKWVSADNTRTILLNSEGEAVANLDRIKRIVSQHTSSSRLSRRRSVTAVDGNEQLYHQQGDPQQSNEKCAIM

Target ORF information:

RefSeq Version	NM_001258963.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lamin, transcript variant B (Lam), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258963.2

ORF Insert Sequence:

ATGTCGAGCA AATCCCGACG TGCTGGCACC GCCACGCCGC AGCCCGGCAA CACCTCCACC 
CCCCGGCCGC CATCGGCGGG TCCGCAGCCG CCGCCGCCGT CCACTCACTC GCAGACGGCC 
TCCAGCCCCC TCAGCCCCAC CCGGCACTCG CGCGTGGCCG AGAAGGTGGA GCTGCAGAAC 
CTGAACGATC GCCTGGCCAC CTACATTGAC CGGGTGCGCA ACCTGGAGAC GGAGAACTCC 
CGCCTCACCA TCGAGGTGCA GACCACCAGG GACACGGTCA CACGCGAGAC CACCAACATC 
AAGAACATCT TCGAGGCCGA GCTGCTGGAG ACGCGCCGTC TGCTCGATGA CACAGCTAGG 
GATCGCGCTC GTGCCGAGAT CGATATCAAG CGTCTCTGGG AGGAGAACGA GGAGCTCAAG 
AACAAGCTGG ACAAGAAGAC CAAGGAGTGC ACCACTGCTG AGGGCAATGT CCGCATGTAC 
GAGTCGCGCG CCAACGAGCT GAACAACAAA TACAACCAGG CCAACGCCGA TCGGAAGAAG 
CTTAACGAAG ACCTGAATGA GGCGCTAAAG GAGCTGGAGA GACTGCGTAA GCAGTTCGAG 
GAAACGCGGA AGAACCTGGA ACAGGAGACA CTGTCGCGCG TTGACCTGGA GAACACCATT 
CAGAGTCTGC GCGAGGAGCT CTCGTTCAAG GATCAGATCC ATTCGCAGGA GATCAATGAG 
TCGCGCCGCA TCAAACAGAC AGAGTATAGC GAGATCGACG GTCGCCTCAG CTCCGAGTAC 
GATGCCAAGT TGAAGCAGTC GCTGCAGGAG CTGCGCGCCC AGTACGAGGA GCAGATGCAG 
ATTAATCGCG ATGAAATCCA GTCCCTCTAC GAGGACAAGA TCCAACGACT GCAAGAGGCC 
GCCGCACGCA CATCCAATTC CACGCACAAG TCCATCGAGG AGCTGCGCTC CACTCGTGTG 
CGTATCGATG CGCTCAACGC CAATATCAAC GAACTGGAGC AAGCCAATGC CGACCTCAAT 
GCGCGGATCC GTGATCTGGA GCGCCAGCTG GACAACGATC GCGAACGCCA CGGTCAAGAG 
ATAGACCTTC TCGAGAAGGA GCTCATTCGG CTGCGCGAAG AGATGACGCA ACAGCTCAAG 
GAGTACCAGG ACCTTATGGA CATCAAGGTC TCCCTGGATT TGGAAATCGC CGCATACGAC 
AAGCTGCTGG TGGGCGAGGA GGCTCGTTTG AACATCACCC CAGCCACCAA CACGGCCACA 
GTGCAGTCCT TTAGCCAGTC GCTGCGCAAC TCCACGCGAG CCACGCCATC GCGTCGCACT 
CCCTCTGCTG CCGTGAAGCG CAAACGCGCC GTGGTCGACG AGTCGGAGGA TCACAGCGTC 
GCCGATTACT ATGTGTCCGC CAGTGCCAAG GGCAACGTGG AGATCAAGGA GATCGATCCC 
GAGGGCAAGT TCGTAAGGCT GTTCAACAAG GGCAGCGAGG AGGTGGCCAT CGGTGGCTGG 
CAGCTGCAGC GGCTAATCAA CGAGAAAGGT CCTTCGACCA CTTACAAGTT CCATCGATCG 
GTGAGGATCG AGCCAAATGG CGTGATCACC GTTTGGTCGG CGGACACCAA GGCCTCGCAC 
GAGCCGCCAT CGAGCCTTGT GATGAAGTCA CAGAAGTGGG TCTCCGCCGA CAACACTAGG 
ACGATTTTGC TGAACTCCGA GGGCGAGGCC GTGGCCAATC TGGATCGCAT CAAGCGCATT 
GTGTCCCAAC ACACATCCTC CTCCCGGCTG AGTCGTCGTC GCAGCGTGAC CGCCGTGGAC 
GGCAATGAGC AGCTCTACCA CCAGCAGGGC GATCCTCAGC AGTCAAACGA GAAGTGCGCC 
ATTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lam ( NM_001258964.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245893.1 Drosophila melanogaster lamin, transcript variant C (Lam), mRNA.

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CloneID	OFa13807
Clone ID Related Accession (Same CDS sequence)	NM_057268.5 , NM_001258963.2 , NM_001258964.2 , NM_001298700.1
Accession Version	NM_001258964.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lamin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001258964.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAGCA AATCCCGACG TGCTGGCACC GCCACGCCGC AGCCCGGCAA CACCTCCACC 
CCCCGGCCGC CATCGGCGGG TCCGCAGCCG CCGCCGCCGT CCACTCACTC GCAGACGGCC 
TCCAGCCCCC TCAGCCCCAC CCGGCACTCG CGCGTGGCCG AGAAGGTGGA GCTGCAGAAC 
CTGAACGATC GCCTGGCCAC CTACATTGAC CGGGTGCGCA ACCTGGAGAC GGAGAACTCC 
CGCCTCACCA TCGAGGTGCA GACCACCAGG GACACGGTCA CACGCGAGAC CACCAACATC 
AAGAACATCT TCGAGGCCGA GCTGCTGGAG ACGCGCCGTC TGCTCGATGA CACAGCTAGG 
GATCGCGCTC GTGCCGAGAT CGATATCAAG CGTCTCTGGG AGGAGAACGA GGAGCTCAAG 
AACAAGCTGG ACAAGAAGAC CAAGGAGTGC ACCACTGCTG AGGGCAATGT CCGCATGTAC 
GAGTCGCGCG CCAACGAGCT GAACAACAAA TACAACCAGG CCAACGCCGA TCGGAAGAAG 
CTTAACGAAG ACCTGAATGA GGCGCTAAAG GAGCTGGAGA GACTGCGTAA GCAGTTCGAG 
GAAACGCGGA AGAACCTGGA ACAGGAGACA CTGTCGCGCG TTGACCTGGA GAACACCATT 
CAGAGTCTGC GCGAGGAGCT CTCGTTCAAG GATCAGATCC ATTCGCAGGA GATCAATGAG 
TCGCGCCGCA TCAAACAGAC AGAGTATAGC GAGATCGACG GTCGCCTCAG CTCCGAGTAC 
GATGCCAAGT TGAAGCAGTC GCTGCAGGAG CTGCGCGCCC AGTACGAGGA GCAGATGCAG 
ATTAATCGCG ATGAAATCCA GTCCCTCTAC GAGGACAAGA TCCAACGACT GCAAGAGGCC 
GCCGCACGCA CATCCAATTC CACGCACAAG TCCATCGAGG AGCTGCGCTC CACTCGTGTG 
CGTATCGATG CGCTCAACGC CAATATCAAC GAACTGGAGC AAGCCAATGC CGACCTCAAT 
GCGCGGATCC GTGATCTGGA GCGCCAGCTG GACAACGATC GCGAACGCCA CGGTCAAGAG 
ATAGACCTTC TCGAGAAGGA GCTCATTCGG CTGCGCGAAG AGATGACGCA ACAGCTCAAG 
GAGTACCAGG ACCTTATGGA CATCAAGGTC TCCCTGGATT TGGAAATCGC CGCATACGAC 
AAGCTGCTGG TGGGCGAGGA GGCTCGTTTG AACATCACCC CAGCCACCAA CACGGCCACA 
GTGCAGTCCT TTAGCCAGTC GCTGCGCAAC TCCACGCGAG CCACGCCATC GCGTCGCACT 
CCCTCTGCTG CCGTGAAGCG CAAACGCGCC GTGGTCGACG AGTCGGAGGA TCACAGCGTC 
GCCGATTACT ATGTGTCCGC CAGTGCCAAG GGCAACGTGG AGATCAAGGA GATCGATCCC 
GAGGGCAAGT TCGTAAGGCT GTTCAACAAG GGCAGCGAGG AGGTGGCCAT CGGTGGCTGG 
CAGCTGCAGC GGCTAATCAA CGAGAAAGGT CCTTCGACCA CTTACAAGTT CCATCGATCG 
GTGAGGATCG AGCCAAATGG CGTGATCACC GTTTGGTCGG CGGACACCAA GGCCTCGCAC 
GAGCCGCCAT CGAGCCTTGT GATGAAGTCA CAGAAGTGGG TCTCCGCCGA CAACACTAGG 
ACGATTTTGC TGAACTCCGA GGGCGAGGCC GTGGCCAATC TGGATCGCAT CAAGCGCATT 
GTGTCCCAAC ACACATCCTC CTCCCGGCTG AGTCGTCGTC GCAGCGTGAC CGCCGTGGAC 
GGCAATGAGC AGCTCTACCA CCAGCAGGGC GATCCTCAGC AGTCAAACGA GAAGTGCGCC 
ATTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245893.1
CDS	502..2370
Translation	MSSKSRRAGTATPQPGNTSTPRPPSAGPQPPPPSTHSQTASSPLSPTRHSRVAEKVELQNLNDRLATYIDRVRNLETENSRLTIEVQTTRDTVTRETTNIKNIFEAELLETRRLLDDTARDRARAEIDIKRLWEENEELKNKLDKKTKECTTAEGNVRMYESRANELNNKYNQANADRKKLNEDLNEALKELERLRKQFEETRKNLEQETLSRVDLENTIQSLREELSFKDQIHSQEINESRRIKQTEYSEIDGRLSSEYDAKLKQSLQELRAQYEEQMQINRDEIQSLYEDKIQRLQEAAARTSNSTHKSIEELRSTRVRIDALNANINELEQANADLNARIRDLERQLDNDRERHGQEIDLLEKELIRLREEMTQQLKEYQDLMDIKVSLDLEIAAYDKLLVGEEARLNITPATNTATVQSFSQSLRNSTRATPSRRTPSAAVKRKRAVVDESEDHSVADYYVSASAKGNVEIKEIDPEGKFVRLFNKGSEEVAIGGWQLQRLINEKGPSTTYKFHRSVRIEPNGVITVWSADTKASHEPPSSLVMKSQKWVSADNTRTILLNSEGEAVANLDRIKRIVSQHTSSSRLSRRRSVTAVDGNEQLYHQQGDPQQSNEKCAIM

Target ORF information:

RefSeq Version	NM_001258964.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lamin, transcript variant C (Lam), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258964.2

ORF Insert Sequence:

ATGTCGAGCA AATCCCGACG TGCTGGCACC GCCACGCCGC AGCCCGGCAA CACCTCCACC 
CCCCGGCCGC CATCGGCGGG TCCGCAGCCG CCGCCGCCGT CCACTCACTC GCAGACGGCC 
TCCAGCCCCC TCAGCCCCAC CCGGCACTCG CGCGTGGCCG AGAAGGTGGA GCTGCAGAAC 
CTGAACGATC GCCTGGCCAC CTACATTGAC CGGGTGCGCA ACCTGGAGAC GGAGAACTCC 
CGCCTCACCA TCGAGGTGCA GACCACCAGG GACACGGTCA CACGCGAGAC CACCAACATC 
AAGAACATCT TCGAGGCCGA GCTGCTGGAG ACGCGCCGTC TGCTCGATGA CACAGCTAGG 
GATCGCGCTC GTGCCGAGAT CGATATCAAG CGTCTCTGGG AGGAGAACGA GGAGCTCAAG 
AACAAGCTGG ACAAGAAGAC CAAGGAGTGC ACCACTGCTG AGGGCAATGT CCGCATGTAC 
GAGTCGCGCG CCAACGAGCT GAACAACAAA TACAACCAGG CCAACGCCGA TCGGAAGAAG 
CTTAACGAAG ACCTGAATGA GGCGCTAAAG GAGCTGGAGA GACTGCGTAA GCAGTTCGAG 
GAAACGCGGA AGAACCTGGA ACAGGAGACA CTGTCGCGCG TTGACCTGGA GAACACCATT 
CAGAGTCTGC GCGAGGAGCT CTCGTTCAAG GATCAGATCC ATTCGCAGGA GATCAATGAG 
TCGCGCCGCA TCAAACAGAC AGAGTATAGC GAGATCGACG GTCGCCTCAG CTCCGAGTAC 
GATGCCAAGT TGAAGCAGTC GCTGCAGGAG CTGCGCGCCC AGTACGAGGA GCAGATGCAG 
ATTAATCGCG ATGAAATCCA GTCCCTCTAC GAGGACAAGA TCCAACGACT GCAAGAGGCC 
GCCGCACGCA CATCCAATTC CACGCACAAG TCCATCGAGG AGCTGCGCTC CACTCGTGTG 
CGTATCGATG CGCTCAACGC CAATATCAAC GAACTGGAGC AAGCCAATGC CGACCTCAAT 
GCGCGGATCC GTGATCTGGA GCGCCAGCTG GACAACGATC GCGAACGCCA CGGTCAAGAG 
ATAGACCTTC TCGAGAAGGA GCTCATTCGG CTGCGCGAAG AGATGACGCA ACAGCTCAAG 
GAGTACCAGG ACCTTATGGA CATCAAGGTC TCCCTGGATT TGGAAATCGC CGCATACGAC 
AAGCTGCTGG TGGGCGAGGA GGCTCGTTTG AACATCACCC CAGCCACCAA CACGGCCACA 
GTGCAGTCCT TTAGCCAGTC GCTGCGCAAC TCCACGCGAG CCACGCCATC GCGTCGCACT 
CCCTCTGCTG CCGTGAAGCG CAAACGCGCC GTGGTCGACG AGTCGGAGGA TCACAGCGTC 
GCCGATTACT ATGTGTCCGC CAGTGCCAAG GGCAACGTGG AGATCAAGGA GATCGATCCC 
GAGGGCAAGT TCGTAAGGCT GTTCAACAAG GGCAGCGAGG AGGTGGCCAT CGGTGGCTGG 
CAGCTGCAGC GGCTAATCAA CGAGAAAGGT CCTTCGACCA CTTACAAGTT CCATCGATCG 
GTGAGGATCG AGCCAAATGG CGTGATCACC GTTTGGTCGG CGGACACCAA GGCCTCGCAC 
GAGCCGCCAT CGAGCCTTGT GATGAAGTCA CAGAAGTGGG TCTCCGCCGA CAACACTAGG 
ACGATTTTGC TGAACTCCGA GGGCGAGGCC GTGGCCAATC TGGATCGCAT CAAGCGCATT 
GTGTCCCAAC ACACATCCTC CTCCCGGCTG AGTCGTCGTC GCAGCGTGAC CGCCGTGGAC 
GGCAATGAGC AGCTCTACCA CCAGCAGGGC GATCCTCAGC AGTCAAACGA GAAGTGCGCC 
ATTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lam ( NM_001298700.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285629.1 Drosophila melanogaster lamin, transcript variant D (Lam), mRNA.

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CloneID	OFa13807
Clone ID Related Accession (Same CDS sequence)	NM_057268.5 , NM_001258963.2 , NM_001258964.2 , NM_001298700.1
Accession Version	NM_001298700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lamin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAGCA AATCCCGACG TGCTGGCACC GCCACGCCGC AGCCCGGCAA CACCTCCACC 
CCCCGGCCGC CATCGGCGGG TCCGCAGCCG CCGCCGCCGT CCACTCACTC GCAGACGGCC 
TCCAGCCCCC TCAGCCCCAC CCGGCACTCG CGCGTGGCCG AGAAGGTGGA GCTGCAGAAC 
CTGAACGATC GCCTGGCCAC CTACATTGAC CGGGTGCGCA ACCTGGAGAC GGAGAACTCC 
CGCCTCACCA TCGAGGTGCA GACCACCAGG GACACGGTCA CACGCGAGAC CACCAACATC 
AAGAACATCT TCGAGGCCGA GCTGCTGGAG ACGCGCCGTC TGCTCGATGA CACAGCTAGG 
GATCGCGCTC GTGCCGAGAT CGATATCAAG CGTCTCTGGG AGGAGAACGA GGAGCTCAAG 
AACAAGCTGG ACAAGAAGAC CAAGGAGTGC ACCACTGCTG AGGGCAATGT CCGCATGTAC 
GAGTCGCGCG CCAACGAGCT GAACAACAAA TACAACCAGG CCAACGCCGA TCGGAAGAAG 
CTTAACGAAG ACCTGAATGA GGCGCTAAAG GAGCTGGAGA GACTGCGTAA GCAGTTCGAG 
GAAACGCGGA AGAACCTGGA ACAGGAGACA CTGTCGCGCG TTGACCTGGA GAACACCATT 
CAGAGTCTGC GCGAGGAGCT CTCGTTCAAG GATCAGATCC ATTCGCAGGA GATCAATGAG 
TCGCGCCGCA TCAAACAGAC AGAGTATAGC GAGATCGACG GTCGCCTCAG CTCCGAGTAC 
GATGCCAAGT TGAAGCAGTC GCTGCAGGAG CTGCGCGCCC AGTACGAGGA GCAGATGCAG 
ATTAATCGCG ATGAAATCCA GTCCCTCTAC GAGGACAAGA TCCAACGACT GCAAGAGGCC 
GCCGCACGCA CATCCAATTC CACGCACAAG TCCATCGAGG AGCTGCGCTC CACTCGTGTG 
CGTATCGATG CGCTCAACGC CAATATCAAC GAACTGGAGC AAGCCAATGC CGACCTCAAT 
GCGCGGATCC GTGATCTGGA GCGCCAGCTG GACAACGATC GCGAACGCCA CGGTCAAGAG 
ATAGACCTTC TCGAGAAGGA GCTCATTCGG CTGCGCGAAG AGATGACGCA ACAGCTCAAG 
GAGTACCAGG ACCTTATGGA CATCAAGGTC TCCCTGGATT TGGAAATCGC CGCATACGAC 
AAGCTGCTGG TGGGCGAGGA GGCTCGTTTG AACATCACCC CAGCCACCAA CACGGCCACA 
GTGCAGTCCT TTAGCCAGTC GCTGCGCAAC TCCACGCGAG CCACGCCATC GCGTCGCACT 
CCCTCTGCTG CCGTGAAGCG CAAACGCGCC GTGGTCGACG AGTCGGAGGA TCACAGCGTC 
GCCGATTACT ATGTGTCCGC CAGTGCCAAG GGCAACGTGG AGATCAAGGA GATCGATCCC 
GAGGGCAAGT TCGTAAGGCT GTTCAACAAG GGCAGCGAGG AGGTGGCCAT CGGTGGCTGG 
CAGCTGCAGC GGCTAATCAA CGAGAAAGGT CCTTCGACCA CTTACAAGTT CCATCGATCG 
GTGAGGATCG AGCCAAATGG CGTGATCACC GTTTGGTCGG CGGACACCAA GGCCTCGCAC 
GAGCCGCCAT CGAGCCTTGT GATGAAGTCA CAGAAGTGGG TCTCCGCCGA CAACACTAGG 
ACGATTTTGC TGAACTCCGA GGGCGAGGCC GTGGCCAATC TGGATCGCAT CAAGCGCATT 
GTGTCCCAAC ACACATCCTC CTCCCGGCTG AGTCGTCGTC GCAGCGTGAC CGCCGTGGAC 
GGCAATGAGC AGCTCTACCA CCAGCAGGGC GATCCTCAGC AGTCAAACGA GAAGTGCGCC 
ATTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285629.1
CDS	189..2057
Translation	MSSKSRRAGTATPQPGNTSTPRPPSAGPQPPPPSTHSQTASSPLSPTRHSRVAEKVELQNLNDRLATYIDRVRNLETENSRLTIEVQTTRDTVTRETTNIKNIFEAELLETRRLLDDTARDRARAEIDIKRLWEENEELKNKLDKKTKECTTAEGNVRMYESRANELNNKYNQANADRKKLNEDLNEALKELERLRKQFEETRKNLEQETLSRVDLENTIQSLREELSFKDQIHSQEINESRRIKQTEYSEIDGRLSSEYDAKLKQSLQELRAQYEEQMQINRDEIQSLYEDKIQRLQEAAARTSNSTHKSIEELRSTRVRIDALNANINELEQANADLNARIRDLERQLDNDRERHGQEIDLLEKELIRLREEMTQQLKEYQDLMDIKVSLDLEIAAYDKLLVGEEARLNITPATNTATVQSFSQSLRNSTRATPSRRTPSAAVKRKRAVVDESEDHSVADYYVSASAKGNVEIKEIDPEGKFVRLFNKGSEEVAIGGWQLQRLINEKGPSTTYKFHRSVRIEPNGVITVWSADTKASHEPPSSLVMKSQKWVSADNTRTILLNSEGEAVANLDRIKRIVSQHTSSSRLSRRRSVTAVDGNEQLYHQQGDPQQSNEKCAIM

Target ORF information:

RefSeq Version	NM_001298700.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lamin, transcript variant D (Lam), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298700.1

ORF Insert Sequence:

ATGTCGAGCA AATCCCGACG TGCTGGCACC GCCACGCCGC AGCCCGGCAA CACCTCCACC 
CCCCGGCCGC CATCGGCGGG TCCGCAGCCG CCGCCGCCGT CCACTCACTC GCAGACGGCC 
TCCAGCCCCC TCAGCCCCAC CCGGCACTCG CGCGTGGCCG AGAAGGTGGA GCTGCAGAAC 
CTGAACGATC GCCTGGCCAC CTACATTGAC CGGGTGCGCA ACCTGGAGAC GGAGAACTCC 
CGCCTCACCA TCGAGGTGCA GACCACCAGG GACACGGTCA CACGCGAGAC CACCAACATC 
AAGAACATCT TCGAGGCCGA GCTGCTGGAG ACGCGCCGTC TGCTCGATGA CACAGCTAGG 
GATCGCGCTC GTGCCGAGAT CGATATCAAG CGTCTCTGGG AGGAGAACGA GGAGCTCAAG 
AACAAGCTGG ACAAGAAGAC CAAGGAGTGC ACCACTGCTG AGGGCAATGT CCGCATGTAC 
GAGTCGCGCG CCAACGAGCT GAACAACAAA TACAACCAGG CCAACGCCGA TCGGAAGAAG 
CTTAACGAAG ACCTGAATGA GGCGCTAAAG GAGCTGGAGA GACTGCGTAA GCAGTTCGAG 
GAAACGCGGA AGAACCTGGA ACAGGAGACA CTGTCGCGCG TTGACCTGGA GAACACCATT 
CAGAGTCTGC GCGAGGAGCT CTCGTTCAAG GATCAGATCC ATTCGCAGGA GATCAATGAG 
TCGCGCCGCA TCAAACAGAC AGAGTATAGC GAGATCGACG GTCGCCTCAG CTCCGAGTAC 
GATGCCAAGT TGAAGCAGTC GCTGCAGGAG CTGCGCGCCC AGTACGAGGA GCAGATGCAG 
ATTAATCGCG ATGAAATCCA GTCCCTCTAC GAGGACAAGA TCCAACGACT GCAAGAGGCC 
GCCGCACGCA CATCCAATTC CACGCACAAG TCCATCGAGG AGCTGCGCTC CACTCGTGTG 
CGTATCGATG CGCTCAACGC CAATATCAAC GAACTGGAGC AAGCCAATGC CGACCTCAAT 
GCGCGGATCC GTGATCTGGA GCGCCAGCTG GACAACGATC GCGAACGCCA CGGTCAAGAG 
ATAGACCTTC TCGAGAAGGA GCTCATTCGG CTGCGCGAAG AGATGACGCA ACAGCTCAAG 
GAGTACCAGG ACCTTATGGA CATCAAGGTC TCCCTGGATT TGGAAATCGC CGCATACGAC 
AAGCTGCTGG TGGGCGAGGA GGCTCGTTTG AACATCACCC CAGCCACCAA CACGGCCACA 
GTGCAGTCCT TTAGCCAGTC GCTGCGCAAC TCCACGCGAG CCACGCCATC GCGTCGCACT 
CCCTCTGCTG CCGTGAAGCG CAAACGCGCC GTGGTCGACG AGTCGGAGGA TCACAGCGTC 
GCCGATTACT ATGTGTCCGC CAGTGCCAAG GGCAACGTGG AGATCAAGGA GATCGATCCC 
GAGGGCAAGT TCGTAAGGCT GTTCAACAAG GGCAGCGAGG AGGTGGCCAT CGGTGGCTGG 
CAGCTGCAGC GGCTAATCAA CGAGAAAGGT CCTTCGACCA CTTACAAGTT CCATCGATCG 
GTGAGGATCG AGCCAAATGG CGTGATCACC GTTTGGTCGG CGGACACCAA GGCCTCGCAC 
GAGCCGCCAT CGAGCCTTGT GATGAAGTCA CAGAAGTGGG TCTCCGCCGA CAACACTAGG 
ACGATTTTGC TGAACTCCGA GGGCGAGGCC GTGGCCAATC TGGATCGCAT CAAGCGCATT 
GTGTCCCAAC ACACATCCTC CTCCCGGCTG AGTCGTCGTC GCAGCGTGAC CGCCGTGGAC 
GGCAATGAGC AGCTCTACCA CCAGCAGGGC GATCCTCAGC AGTCAAACGA GAAGTGCGCC 
ATTATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Lam cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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