Hel25E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hel25E
Entrez Gene ID	33781
Full Name	Helicase at 25E
Synonyms	CG7269,Dbp25F,DmRH4,Dmel\CG7269,HEL,HEL25E,Hel,UAP56,WM6,Wm6,hel,jf26,l(2)25Eb,l(2)gdh-12,l(2)gdh12,l(2)jf26,l(2)k11511,sz,uap56
General protein information	Preferred Names Helicase at 25E Names CG7269 gene product from transcript CG7269-RC CG7269-PA CG7269-PB CG7269-PC CG7269-PD Hel25E-PA Hel25E-PB Hel25E-PC Hel25E-PD RNA helicase helicase helicase at 25E lethal(2)25Eb
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 25E6-25E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_164647.1	NP_723090.1	helicase at 25E, isoform B
NM_164648.2	NP_723091.1	helicase at 25E, isoform C
NM_001273171.1	NP_001260100.1	helicase at 25E, isoform D
NM_164646.3	NP_723089.1	helicase at 25E, isoform A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ddx39b	NP_579834.2
Danio rerio (zebrafish)	bat1	NP_957237.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009075	XP_319825.4
Oryza sativa (rice)	Os01g0550000	NP_001043298.1
Homo sapiens (human)	DDX39B	NP_004631.1
Canis lupus familiaris (dog)	DDX39B	NP_001014399.1
Pan troglodytes (chimpanzee)	DDX39B	NP_001107635.1
	DDX39B	NP_001040608.1
Kluyveromyces lactis	KLLA0E21935g	XP_454944.1
Arabidopsis thaliana (thale cress)	AT5G11170	NP_568244.2
Xenopus tropicalis (tropical clawed frog)	ddx39b	NP_989072.1
Bos taurus (cattle)	DDX39B	NP_001028801.1
Mus musculus (house mouse)	Ddx39b	NP_001239386.1
Drosophila melanogaster (fruit fly)	Hel25E	NP_723089.1
Schizosaccharomyces pombe (fission yeast)	uap56	NP_594261.1
Magnaporthe oryzae (rice blast fungus)	MGG_02806	XP_003720935.1
Neurospora crassa	NCU09076	XP_964893.2
Saccharomyces cerevisiae (baker's yeast)	SUB2	NP_010199.1
Eremothecium gossypii	AGOS_ADR260C	NP_984356.1
Oryza sativa (rice)	Os01g0549700	NP_001043297.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study presents evidence that UAP56 and Vas are cluster transcript binding proteins that organize a piRNA-processing compartment that spans the nuclear envelope.
Title: UAP56 couples piRNA clusters to the perinuclear transposon silencing machinery.

UAP56 RNA helicase is required for axis specification and cytoplasmic mRNA localization.
Title: UAP56 RNA helicase is required for axis specification and cytoplasmic mRNA localization in Drosophila.

The following Hel25E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hel25E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13806	NM_001273171.1 Latest version!	Drosophila melanogaster helicase at 25E, transcript variant D (Hel25E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa16282	NM_164646.3 Latest version!	Drosophila melanogaster helicase at 25E, transcript variant A (Hel25E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa16282	NM_164648.2 Latest version!	Drosophila melanogaster helicase at 25E, transcript variant C (Hel25E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa16282	NM_164647.1 Latest version!	Drosophila melanogaster helicase at 25E, transcript variant B (Hel25E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hel25E ( NM_001273171.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260100.1 Drosophila melanogaster helicase at 25E, transcript variant D (Hel25E), mRNA.

Price & Availability↑

CloneID	OFa13806
Clone ID Related Accession (Same CDS sequence)	NM_001273171.1
Accession Version	NM_001273171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	helicase at 25E, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT 
TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC 
TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC 
ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG 
CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC 
GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC 
AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC 
GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG 
AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG 
CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA 
GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG 
CAAGATGTAA ATATCAACGT GTTCATAACC AAAATGCTCG TACTTCTCCA CCTCGCATCC 
ACCGCTATCA CGACAATTAG TTATTATTTT GTTAATGCCA CGCGATATCG AACATTCGAT 
CTTGATCCCC TCCGTATAAG CCAACCACCA TCTTGCAAAT GGTTAAACGC GTACAACCAA 
ACGTCCAGCG CTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260100.1
CDS	188..1105
Translation	MADNDDLLDYEDEEQTETTAVENQEAPKKDVKGTYVSIHSSGFRDFLLKPEILRAIVDCGFEHPSEVQHECIPQAVLGMDILCQAKSGMGKTAVFVLATLQQLEPSDNNTCHVLVMCHTRELAFQISKEYERFSKYMPTVKVAVFFGGMAIQKDEETLKSGTPHIVVGTPGRILALIRNKKLNLKLLKHFVLDECDKMLEQLDMRRDVQEIFRSTPHGKQVMMFSATLSKDIRPVCKKFMQDVNINVFITKMLVLLHLASTAITTISYYFVNATRYRTFDLDPLRISQPPSCKWLNAYNQTSSAL

Target ORF information:

RefSeq Version	NM_001273171.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster helicase at 25E, transcript variant D (Hel25E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273171.1

ORF Insert Sequence:

ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT 
TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC 
TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC 
ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG 
CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC 
GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC 
AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC 
GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG 
AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG 
CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA 
GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG 
CAAGATGTAA ATATCAACGT GTTCATAACC AAAATGCTCG TACTTCTCCA CCTCGCATCC 
ACCGCTATCA CGACAATTAG TTATTATTTT GTTAATGCCA CGCGATATCG AACATTCGAT 
CTTGATCCCC TCCGTATAAG CCAACCACCA TCTTGCAAAT GGTTAAACGC GTACAACCAA 
ACGTCCAGCG CTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hel25E ( NM_164647.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723090.1 Drosophila melanogaster helicase at 25E, transcript variant B (Hel25E), mRNA.

Price & Availability↑

CloneID	OFa16282
Clone ID Related Accession (Same CDS sequence)	NM_164647.1 , NM_164648.2 , NM_164646.3
Accession Version	NM_164647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	helicase at 25E, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT 
TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC 
TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC 
ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG 
CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC 
GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC 
AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC 
GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG 
AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG 
CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA 
GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG 
CAAGATCCCA TGGAGGTCTA CGTCGACGAT GAGGCCAAGC TGACGCTGCA CGGACTGCAG 
CAGCACTACG TCAATCTGAA GGAGAACGAG AAGAACAAGA AACTGTTCGA ACTGCTCGAC 
GTGCTCGAGT TCAATCAGGT GGTCATCTTT GTGAAGTCTG TGCAACGTTG CGTGGCTCTG 
TCGCAGCTGC TGACGGAGCA GAACTTCCCC GCCATCGGCA TCCATCGTGG GATGACCCAG 
GAGGAGCGTC TGAATCGCTA CCAGCAGTTC AAGGACTTCC AGAAGCGCAT TCTGGTGGCC 
ACCAATCTCT TTGGCCGCGG CATGGACATC GAGCGTGTGA ACATCGTGTT CAACTACGAC 
ATGCCCGAGG ATTCCGACAC CTACTTGCAT CGCGTGGCCC GTGCCGGTCG CTTCGGCACC 
AAGGGACTGG CAATCACATT CGTTTCGGAC GAGAACGACG CCAAGATACT TAACGAAGTA 
CAGGATCGTT TCGATGTGAA CATCAGTGAG CTGCCCGAGG AAATCGATCT CTCTACATAC 
ATTGAGGGAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723090.1
CDS	78..1352
Translation	MADNDDLLDYEDEEQTETTAVENQEAPKKDVKGTYVSIHSSGFRDFLLKPEILRAIVDCGFEHPSEVQHECIPQAVLGMDILCQAKSGMGKTAVFVLATLQQLEPSDNNTCHVLVMCHTRELAFQISKEYERFSKYMPTVKVAVFFGGMAIQKDEETLKSGTPHIVVGTPGRILALIRNKKLNLKLLKHFVLDECDKMLEQLDMRRDVQEIFRSTPHGKQVMMFSATLSKDIRPVCKKFMQDPMEVYVDDEAKLTLHGLQQHYVNLKENEKNKKLFELLDVLEFNQVVIFVKSVQRCVALSQLLTEQNFPAIGIHRGMTQEERLNRYQQFKDFQKRILVATNLFGRGMDIERVNIVFNYDMPEDSDTYLHRVARAGRFGTKGLAITFVSDENDAKILNEVQDRFDVNISELPEEIDLSTYIEGR

Target ORF information:

RefSeq Version	NM_164647.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster helicase at 25E, transcript variant B (Hel25E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164647.1

ORF Insert Sequence:

ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT 
TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC 
TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC 
ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG 
CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC 
GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC 
AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC 
GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG 
AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG 
CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA 
GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG 
CAAGATCCCA TGGAGGTCTA CGTCGACGAT GAGGCCAAGC TGACGCTGCA CGGACTGCAG 
CAGCACTACG TCAATCTGAA GGAGAACGAG AAGAACAAGA AACTGTTCGA ACTGCTCGAC 
GTGCTCGAGT TCAATCAGGT GGTCATCTTT GTGAAGTCTG TGCAACGTTG CGTGGCTCTG 
TCGCAGCTGC TGACGGAGCA GAACTTCCCC GCCATCGGCA TCCATCGTGG GATGACCCAG 
GAGGAGCGTC TGAATCGCTA CCAGCAGTTC AAGGACTTCC AGAAGCGCAT TCTGGTGGCC 
ACCAATCTCT TTGGCCGCGG CATGGACATC GAGCGTGTGA ACATCGTGTT CAACTACGAC 
ATGCCCGAGG ATTCCGACAC CTACTTGCAT CGCGTGGCCC GTGCCGGTCG CTTCGGCACC 
AAGGGACTGG CAATCACATT CGTTTCGGAC GAGAACGACG CCAAGATACT TAACGAAGTA 
CAGGATCGTT TCGATGTGAA CATCAGTGAG CTGCCCGAGG AAATCGATCT CTCTACATAC 
ATTGAGGGAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hel25E ( NM_164648.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723091.1 Drosophila melanogaster helicase at 25E, transcript variant C (Hel25E), mRNA.

Price & Availability↑

CloneID	OFa16282
Clone ID Related Accession (Same CDS sequence)	NM_164647.1 , NM_164648.2 , NM_164646.3
Accession Version	NM_164648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	helicase at 25E, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164648.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT 
TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC 
TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC 
ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG 
CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC 
GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC 
AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC 
GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG 
AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG 
CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA 
GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG 
CAAGATCCCA TGGAGGTCTA CGTCGACGAT GAGGCCAAGC TGACGCTGCA CGGACTGCAG 
CAGCACTACG TCAATCTGAA GGAGAACGAG AAGAACAAGA AACTGTTCGA ACTGCTCGAC 
GTGCTCGAGT TCAATCAGGT GGTCATCTTT GTGAAGTCTG TGCAACGTTG CGTGGCTCTG 
TCGCAGCTGC TGACGGAGCA GAACTTCCCC GCCATCGGCA TCCATCGTGG GATGACCCAG 
GAGGAGCGTC TGAATCGCTA CCAGCAGTTC AAGGACTTCC AGAAGCGCAT TCTGGTGGCC 
ACCAATCTCT TTGGCCGCGG CATGGACATC GAGCGTGTGA ACATCGTGTT CAACTACGAC 
ATGCCCGAGG ATTCCGACAC CTACTTGCAT CGCGTGGCCC GTGCCGGTCG CTTCGGCACC 
AAGGGACTGG CAATCACATT CGTTTCGGAC GAGAACGACG CCAAGATACT TAACGAAGTA 
CAGGATCGTT TCGATGTGAA CATCAGTGAG CTGCCCGAGG AAATCGATCT CTCTACATAC 
ATTGAGGGAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723091.1
CDS	181..1455
Translation	MADNDDLLDYEDEEQTETTAVENQEAPKKDVKGTYVSIHSSGFRDFLLKPEILRAIVDCGFEHPSEVQHECIPQAVLGMDILCQAKSGMGKTAVFVLATLQQLEPSDNNTCHVLVMCHTRELAFQISKEYERFSKYMPTVKVAVFFGGMAIQKDEETLKSGTPHIVVGTPGRILALIRNKKLNLKLLKHFVLDECDKMLEQLDMRRDVQEIFRSTPHGKQVMMFSATLSKDIRPVCKKFMQDPMEVYVDDEAKLTLHGLQQHYVNLKENEKNKKLFELLDVLEFNQVVIFVKSVQRCVALSQLLTEQNFPAIGIHRGMTQEERLNRYQQFKDFQKRILVATNLFGRGMDIERVNIVFNYDMPEDSDTYLHRVARAGRFGTKGLAITFVSDENDAKILNEVQDRFDVNISELPEEIDLSTYIEGR

Target ORF information:

RefSeq Version	NM_164648.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster helicase at 25E, transcript variant C (Hel25E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164648.2

ORF Insert Sequence:

ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT 
TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC 
TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC 
ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG 
CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC 
GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC 
AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC 
GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG 
AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG 
CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA 
GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG 
CAAGATCCCA TGGAGGTCTA CGTCGACGAT GAGGCCAAGC TGACGCTGCA CGGACTGCAG 
CAGCACTACG TCAATCTGAA GGAGAACGAG AAGAACAAGA AACTGTTCGA ACTGCTCGAC 
GTGCTCGAGT TCAATCAGGT GGTCATCTTT GTGAAGTCTG TGCAACGTTG CGTGGCTCTG 
TCGCAGCTGC TGACGGAGCA GAACTTCCCC GCCATCGGCA TCCATCGTGG GATGACCCAG 
GAGGAGCGTC TGAATCGCTA CCAGCAGTTC AAGGACTTCC AGAAGCGCAT TCTGGTGGCC 
ACCAATCTCT TTGGCCGCGG CATGGACATC GAGCGTGTGA ACATCGTGTT CAACTACGAC 
ATGCCCGAGG ATTCCGACAC CTACTTGCAT CGCGTGGCCC GTGCCGGTCG CTTCGGCACC 
AAGGGACTGG CAATCACATT CGTTTCGGAC GAGAACGACG CCAAGATACT TAACGAAGTA 
CAGGATCGTT TCGATGTGAA CATCAGTGAG CTGCCCGAGG AAATCGATCT CTCTACATAC 
ATTGAGGGAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hel25E ( NM_164646.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723089.1 Drosophila melanogaster helicase at 25E, transcript variant A (Hel25E), mRNA.

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CloneID	OFa16282
Clone ID Related Accession (Same CDS sequence)	NM_164647.1 , NM_164648.2 , NM_164646.3
Accession Version	NM_164646.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	helicase at 25E, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164646.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT 
TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC 
TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC 
ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG 
CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC 
GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC 
AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC 
GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG 
AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG 
CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA 
GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG 
CAAGATCCCA TGGAGGTCTA CGTCGACGAT GAGGCCAAGC TGACGCTGCA CGGACTGCAG 
CAGCACTACG TCAATCTGAA GGAGAACGAG AAGAACAAGA AACTGTTCGA ACTGCTCGAC 
GTGCTCGAGT TCAATCAGGT GGTCATCTTT GTGAAGTCTG TGCAACGTTG CGTGGCTCTG 
TCGCAGCTGC TGACGGAGCA GAACTTCCCC GCCATCGGCA TCCATCGTGG GATGACCCAG 
GAGGAGCGTC TGAATCGCTA CCAGCAGTTC AAGGACTTCC AGAAGCGCAT TCTGGTGGCC 
ACCAATCTCT TTGGCCGCGG CATGGACATC GAGCGTGTGA ACATCGTGTT CAACTACGAC 
ATGCCCGAGG ATTCCGACAC CTACTTGCAT CGCGTGGCCC GTGCCGGTCG CTTCGGCACC 
AAGGGACTGG CAATCACATT CGTTTCGGAC GAGAACGACG CCAAGATACT TAACGAAGTA 
CAGGATCGTT TCGATGTGAA CATCAGTGAG CTGCCCGAGG AAATCGATCT CTCTACATAC 
ATTGAGGGAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723089.1
CDS	188..1462
Translation	MADNDDLLDYEDEEQTETTAVENQEAPKKDVKGTYVSIHSSGFRDFLLKPEILRAIVDCGFEHPSEVQHECIPQAVLGMDILCQAKSGMGKTAVFVLATLQQLEPSDNNTCHVLVMCHTRELAFQISKEYERFSKYMPTVKVAVFFGGMAIQKDEETLKSGTPHIVVGTPGRILALIRNKKLNLKLLKHFVLDECDKMLEQLDMRRDVQEIFRSTPHGKQVMMFSATLSKDIRPVCKKFMQDPMEVYVDDEAKLTLHGLQQHYVNLKENEKNKKLFELLDVLEFNQVVIFVKSVQRCVALSQLLTEQNFPAIGIHRGMTQEERLNRYQQFKDFQKRILVATNLFGRGMDIERVNIVFNYDMPEDSDTYLHRVARAGRFGTKGLAITFVSDENDAKILNEVQDRFDVNISELPEEIDLSTYIEGR

Target ORF information:

RefSeq Version	NM_164646.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster helicase at 25E, transcript variant A (Hel25E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164646.3

ORF Insert Sequence:

ATGGCCGACA ATGACGATCT TTTGGACTAC GAGGATGAGG AGCAGACCGA GACCACTGCG 
GTCGAAAACC AGGAGGCCCC CAAGAAGGAT GTCAAGGGCA CCTATGTGTC CATTCACAGT 
TCCGGCTTCC GCGATTTCCT CCTGAAACCG GAGATCCTGC GCGCCATCGT GGACTGCGGC 
TTCGAGCATC CCTCGGAGGT TCAGCACGAG TGCATTCCGC AGGCCGTACT GGGCATGGAC 
ATCCTCTGTC AGGCCAAGTC CGGCATGGGT AAGACCGCCG TCTTCGTTCT GGCCACGCTG 
CAGCAGCTGG AGCCGTCGGA CAACAACACC TGCCACGTCC TGGTCATGTG CCACACCCGC 
GAGCTGGCCT TCCAGATCAG CAAGGAGTAT GAGCGATTCT CCAAGTACAT GCCCACAGTC 
AAGGTGGCTG TCTTCTTTGG CGGAATGGCT ATTCAAAAGG ACGAGGAGAC CCTCAAGAGC 
GGCACCCCGC ATATTGTGGT GGGCACCCCT GGCCGAATTC TCGCCCTCAT TCGCAACAAG 
AAACTTAATC TGAAGCTTTT GAAGCACTTT GTGCTCGACG AGTGCGACAA GATGCTGGAG 
CAGCTGGATA TGCGTCGTGA CGTTCAAGAG ATTTTCCGTA GCACGCCGCA CGGCAAACAA 
GTGATGATGT TCTCTGCCAC ATTGAGCAAG GACATTCGTC CCGTTTGCAA AAAGTTCATG 
CAAGATCCCA TGGAGGTCTA CGTCGACGAT GAGGCCAAGC TGACGCTGCA CGGACTGCAG 
CAGCACTACG TCAATCTGAA GGAGAACGAG AAGAACAAGA AACTGTTCGA ACTGCTCGAC 
GTGCTCGAGT TCAATCAGGT GGTCATCTTT GTGAAGTCTG TGCAACGTTG CGTGGCTCTG 
TCGCAGCTGC TGACGGAGCA GAACTTCCCC GCCATCGGCA TCCATCGTGG GATGACCCAG 
GAGGAGCGTC TGAATCGCTA CCAGCAGTTC AAGGACTTCC AGAAGCGCAT TCTGGTGGCC 
ACCAATCTCT TTGGCCGCGG CATGGACATC GAGCGTGTGA ACATCGTGTT CAACTACGAC 
ATGCCCGAGG ATTCCGACAC CTACTTGCAT CGCGTGGCCC GTGCCGGTCG CTTCGGCACC 
AAGGGACTGG CAATCACATT CGTTTCGGAC GAGAACGACG CCAAGATACT TAACGAAGTA 
CAGGATCGTT TCGATGTGAA CATCAGTGAG CTGCCCGAGG AAATCGATCT CTCTACATAC 
ATTGAGGGAC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hel25E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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