Marcal1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Marcal1
Entrez Gene ID	33709
Full Name	CG3753 gene product from transcript CG3753-RA
Synonyms	CG3753,Dmel\CG3753,HARP,MARCAL1,Marca1,dmHARP
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 25B4-25B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_135039.2	NP_608883.1	Marcal1

Pathways from BioSystems

Homologies

	SMARCAL1	XP_001086594.1
Xenopus tropicalis (tropical clawed frog)	smarcal1	NP_001072923.1
Pan troglodytes (chimpanzee)	SMARCAL1	XP_001153124.1
Bos taurus (cattle)	SMARCAL1	NP_788839.1
Rattus norvegicus (Norway rat)	Smarcal1	NP_001101692.1
Gallus gallus (chicken)	SMARCAL1	XP_422070.3
Mus musculus (house mouse)	Smarcal1	NP_061287.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008426	XP_317019.4
Homo sapiens (human)	SMARCAL1	NP_001120679.1
Canis lupus familiaris (dog)	SMARCAL1	XP_005640678.1
Caenorhabditis elegans (roundworm)	C16A3.1	NP_741192.1
Danio rerio (zebrafish)	smarcal1	NP_001120938.1
Drosophila melanogaster (fruit fly)	Marcal1	NP_608883.1
Arabidopsis thaliana (thale cress)	CHR18	NP_175265.3
Oryza sativa (rice)	Os07g0636200	NP_001060393.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

limination of the ATP-binding activity of Marcal1 also reduced annealing-dependent repair, suggesting that the annealing activity requires translocation along DNA. Unlike the null mutant, however, the ATP-binding defect mutant showed reduced end joining, shedding light on the interaction between SDSA and end-joining pathways.
Title: Annealing of Complementary DNA Sequences During Double-Strand Break Repair in

deficiency of a SMARCAL1 ortholog altering the chromatin structure of a gene
Title: Chromatin changes in SMARCAL1 deficiency: A hypothesis for the gene expression alterations of Schimke immuno-osseous dysplasia.

The following Marcal1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Marcal1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16228	NM_135039.2 Latest version!	Drosophila melanogaster Marcal1 (Marcal1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Marcal1 ( NM_135039.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608883.1 Drosophila melanogaster Marcal1 (Marcal1), mRNA.

Price & Availability↑

CloneID	OFa16228
Clone ID Related Accession (Same CDS sequence)	NM_135039.2
Accession Version	NM_135039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Marcal1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_135039.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCACTT GCAGTTCATC CGAGATAGCG GAGAAGAAGC GCATTGCGCT GGCCAAATTG 
CAGGCCAAGA AATCCCAGCT GCTGGCCAGC GCACCCGCAA CCAATGGAAA ATCAACAACG 
TCTGCAACAG GTGCGACGCA ACATGCGAAT AATGGAAAAT CAAATCCCAA TCAACCGCAG 
GCCAAATCCC CATTGAATTT CTACCGATCA CCGACGGGGG AGCAGAAGAA GATCAACAGG 
AGCGGTCCCA CGCCCGGTGA CAACAAGAGC TCATCCTTTC TGAACGCTCT GAAAGCAATC 
AAACAGACAT CCAACCGGGA ATTGTCCCGC GGAGCAGCAC ATCCTTACCA GCGGCCAAAT 
GGAGGGAACG AAAGAAACAA GCCCACTCTT TCACTGAGTT CGGATAAGGA GAAACCGGTG 
GCTGTGCTAC TCGGAAACTC CATCACGTGT AATCTCTACT TGATCAGCAC ACATCGGTTT 
GCTGCCCAAA CCTCAGGATA TCACGAACAG CTCGTGACCG TGTTCAAGAA CATGCCCACC 
AAGTGCTACG ATGGCCAAAC CCGTATCTGG AGTTTCGATC TGTCGGACTA CCAATCCCTG 
AAAACTCATG CAGCGGATCT AAAGCCATAT GTTCACATGA ATGGCATTCC GAAGAAGGTG 
CTGGATCTCT GCGGCCAGCC GCCAGTAGTT CCGGAAAGAA GTGTTTTAGC TTCCATAGAA 
CCGAAGCTGG CGGACCAGCT AATGCCCTTC CAACAGGATG GGGTATGCTT TGCCATAGCC 
CAAAAGGGAC GCATTATGAT CTGTGATGAA ATGGGCCTGG GCAAAACCTA TCAGGCCTTG 
GCCGTAGCCG ACTATTTCAA GGATGATTGG CCCTTGCTTG TCTGCACCAC CGCTTCTACG 
AGAGACAGTT GGGCGAAACA CATAATGGAT CTACTGCCGA AGGTGCCCAT CCACTATGTC 
CAGGTGCTGA ACAACAATCA GCTATATGTG GGTGAGGCCA AGGTGCTAAT CACCAGCTAC 
AATATGATGG AGCGACACGA AGATAAGCTG ATGCAGCGGA AGTTTGGTTT CATCATCTTC 
GACGAGTCGC ACACGCTGAA GAACAGCAAG GCCAAGTGCA CGACAACGGC CAAAAGACTC 
ACGGATCAGG CGAAGCGGGT GGTTTTGCTA TCCGGCACAC CGGCTCTCTC CCGGCCATTG 
GAACTTTTTA CGCAACTGCA AATGATTGAT GGCAAATTCA TGAATTTCAT GGAGTTCACT 
ACTCGTTATT GTGATGGAAA GCAGTCAACT TTTGGTTGGG ATGCCAATGG TCAATCGAAT 
TTGGAGGAAC TGAAGGTCAT ACTTCACCTT AAATATATGC TGAGGCGAAC GAAAGTGGAG 
GTGCTGCCTC AACTCGCTGA GAAGAACCGT GAGACTGTCG TCCTGGATCC CGCCCTAGTG 
TGGACCAATG CGGAGACCAA GGAAACCTTG GATGCGTTTA ACAAAGAGCT TAAAACCGCT 
AAAGGCAGGG CAACGGAAGA GATCTTACTC CGTTTCTATG CACGCACTGC CGAAGTGAAA 
ACTCGAGCGG TCTGCGCCTA CCTAAAAACC TTAGTTAAGG AGCAGAAAAA GTTCATTATC 
TTTGCCCACC ATCGTGTCAT GATGGACGCC ATTAGCGACT TCCTCAGTGG ATTGAAAGTA 
CACTACATAC GTATTGATGG ACAAACGCGT AGCGATCACA GGTCGGATTC TGTGGACACG 
TTCCAAAAGA AGAGCAGTTG TAAGGTGGCC CTGCTCTCCC TAAAAGCCTG CAATTCAGGA 
ATAACGCTAA CTGCAGCTGA GATCATAGTG TTCGCTGAAC TGGACTGGAA TCCTAGCACT 
CTTGCCCAGG CGGAGAGCCG TGCCCATCGC ATTGGTCAGA CAAAACCGGT GATTTGTCGC 
TATCTGATTG CTCACAATAC GGCAGACGAT ATAATCTGGA ACATGCTGAA AAATAAGCAA 
GAGGTGCTTA GTAAAGTGGG CATCTTTGCC GAAAATCTTC AGAAAGCAAC ACACACAGCA 
GCACCCACTT CATCCCACAA GATCGAGGAG TACTTCTCGC CCTCCACGTC CACCTCCCTT 
GAACCGGAAA GGAATAGCAT AAAACAATAT TTTTCAACTA TTCCAGCTAA AGAGCCGCCT 
GAGCAAAATA ATAATACAGA AATGACCAAA GTAAACAAGG CTGAGTCGGA TATCGCGGCG 
TTCTTTAACG ATGATGATGA TGAGGCTTTT TTGGAATTAG ATATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608883.1
CDS	75..2342
Translation	MSTCSSSEIAEKKRIALAKLQAKKSQLLASAPATNGKSTTSATGATQHANNGKSNPNQPQAKSPLNFYRSPTGEQKKINRSGPTPGDNKSSSFLNALKAIKQTSNRELSRGAAHPYQRPNGGNERNKPTLSLSSDKEKPVAVLLGNSITCNLYLISTHRFAAQTSGYHEQLVTVFKNMPTKCYDGQTRIWSFDLSDYQSLKTHAADLKPYVHMNGIPKKVLDLCGQPPVVPERSVLASIEPKLADQLMPFQQDGVCFAIAQKGRIMICDEMGLGKTYQALAVADYFKDDWPLLVCTTASTRDSWAKHIMDLLPKVPIHYVQVLNNNQLYVGEAKVLITSYNMMERHEDKLMQRKFGFIIFDESHTLKNSKAKCTTTAKRLTDQAKRVVLLSGTPALSRPLELFTQLQMIDGKFMNFMEFTTRYCDGKQSTFGWDANGQSNLEELKVILHLKYMLRRTKVEVLPQLAEKNRETVVLDPALVWTNAETKETLDAFNKELKTAKGRATEEILLRFYARTAEVKTRAVCAYLKTLVKEQKKFIIFAHHRVMMDAISDFLSGLKVHYIRIDGQTRSDHRSDSVDTFQKKSSCKVALLSLKACNSGITLTAAEIIVFAELDWNPSTLAQAESRAHRIGQTKPVICRYLIAHNTADDIIWNMLKNKQEVLSKVGIFAENLQKATHTAAPTSSHKIEEYFSPSTSTSLEPERNSIKQYFSTIPAKEPPEQNNNTEMTKVNKAESDIAAFFNDDDDEAFLELDI

Target ORF information:

RefSeq Version	NM_135039.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Marcal1 (Marcal1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135039.2

ORF Insert Sequence:

ATGTCCACTT GCAGTTCATC CGAGATAGCG GAGAAGAAGC GCATTGCGCT GGCCAAATTG 
CAGGCCAAGA AATCCCAGCT GCTGGCCAGC GCACCCGCAA CCAATGGAAA ATCAACAACG 
TCTGCAACAG GTGCGACGCA ACATGCGAAT AATGGAAAAT CAAATCCCAA TCAACCGCAG 
GCCAAATCCC CATTGAATTT CTACCGATCA CCGACGGGGG AGCAGAAGAA GATCAACAGG 
AGCGGTCCCA CGCCCGGTGA CAACAAGAGC TCATCCTTTC TGAACGCTCT GAAAGCAATC 
AAACAGACAT CCAACCGGGA ATTGTCCCGC GGAGCAGCAC ATCCTTACCA GCGGCCAAAT 
GGAGGGAACG AAAGAAACAA GCCCACTCTT TCACTGAGTT CGGATAAGGA GAAACCGGTG 
GCTGTGCTAC TCGGAAACTC CATCACGTGT AATCTCTACT TGATCAGCAC ACATCGGTTT 
GCTGCCCAAA CCTCAGGATA TCACGAACAG CTCGTGACCG TGTTCAAGAA CATGCCCACC 
AAGTGCTACG ATGGCCAAAC CCGTATCTGG AGTTTCGATC TGTCGGACTA CCAATCCCTG 
AAAACTCATG CAGCGGATCT AAAGCCATAT GTTCACATGA ATGGCATTCC GAAGAAGGTG 
CTGGATCTCT GCGGCCAGCC GCCAGTAGTT CCGGAAAGAA GTGTTTTAGC TTCCATAGAA 
CCGAAGCTGG CGGACCAGCT AATGCCCTTC CAACAGGATG GGGTATGCTT TGCCATAGCC 
CAAAAGGGAC GCATTATGAT CTGTGATGAA ATGGGCCTGG GCAAAACCTA TCAGGCCTTG 
GCCGTAGCCG ACTATTTCAA GGATGATTGG CCCTTGCTTG TCTGCACCAC CGCTTCTACG 
AGAGACAGTT GGGCGAAACA CATAATGGAT CTACTGCCGA AGGTGCCCAT CCACTATGTC 
CAGGTGCTGA ACAACAATCA GCTATATGTG GGTGAGGCCA AGGTGCTAAT CACCAGCTAC 
AATATGATGG AGCGACACGA AGATAAGCTG ATGCAGCGGA AGTTTGGTTT CATCATCTTC 
GACGAGTCGC ACACGCTGAA GAACAGCAAG GCCAAGTGCA CGACAACGGC CAAAAGACTC 
ACGGATCAGG CGAAGCGGGT GGTTTTGCTA TCCGGCACAC CGGCTCTCTC CCGGCCATTG 
GAACTTTTTA CGCAACTGCA AATGATTGAT GGCAAATTCA TGAATTTCAT GGAGTTCACT 
ACTCGTTATT GTGATGGAAA GCAGTCAACT TTTGGTTGGG ATGCCAATGG TCAATCGAAT 
TTGGAGGAAC TGAAGGTCAT ACTTCACCTT AAATATATGC TGAGGCGAAC GAAAGTGGAG 
GTGCTGCCTC AACTCGCTGA GAAGAACCGT GAGACTGTCG TCCTGGATCC CGCCCTAGTG 
TGGACCAATG CGGAGACCAA GGAAACCTTG GATGCGTTTA ACAAAGAGCT TAAAACCGCT 
AAAGGCAGGG CAACGGAAGA GATCTTACTC CGTTTCTATG CACGCACTGC CGAAGTGAAA 
ACTCGAGCGG TCTGCGCCTA CCTAAAAACC TTAGTTAAGG AGCAGAAAAA GTTCATTATC 
TTTGCCCACC ATCGTGTCAT GATGGACGCC ATTAGCGACT TCCTCAGTGG ATTGAAAGTA 
CACTACATAC GTATTGATGG ACAAACGCGT AGCGATCACA GGTCGGATTC TGTGGACACG 
TTCCAAAAGA AGAGCAGTTG TAAGGTGGCC CTGCTCTCCC TAAAAGCCTG CAATTCAGGA 
ATAACGCTAA CTGCAGCTGA GATCATAGTG TTCGCTGAAC TGGACTGGAA TCCTAGCACT 
CTTGCCCAGG CGGAGAGCCG TGCCCATCGC ATTGGTCAGA CAAAACCGGT GATTTGTCGC 
TATCTGATTG CTCACAATAC GGCAGACGAT ATAATCTGGA ACATGCTGAA AAATAAGCAA 
GAGGTGCTTA GTAAAGTGGG CATCTTTGCC GAAAATCTTC AGAAAGCAAC ACACACAGCA 
GCACCCACTT CATCCCACAA GATCGAGGAG TACTTCTCGC CCTCCACGTC CACCTCCCTT 
GAACCGGAAA GGAATAGCAT AAAACAATAT TTTTCAACTA TTCCAGCTAA AGAGCCGCCT 
GAGCAAAATA ATAATACAGA AATGACCAAA GTAAACAAGG CTGAGTCGGA TATCGCGGCG 
TTCTTTAACG ATGATGATGA TGAGGCTTTT TTGGAATTAG ATATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Marcal1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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