Cf2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cf2
Entrez Gene ID	33692
Full Name	Chorion factor 2
Synonyms	BcDNA:GM09668,CF2,CF2.5,CG11924,Dmel\CG11924,cf2
General protein information	Preferred Names Chorion factor 2 Names CG11924 gene product from transcript CG11924-RE CG11924-PA CG11924-PB CG11924-PC CG11924-PE CG11924-PF Cf2-PA Cf2-PB Cf2-PC Cf2-PE Cf2-PF chorion Factor-2 chorion factor 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 25B1-25B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_175965.2	NP_787979.1	chorion factor 2, isoform C
NM_078750.4	NP_523474.1	chorion factor 2, isoform A
NM_175964.2	NP_787978.1	chorion factor 2, isoform B
NM_001273128.1	NP_001260057.1	chorion factor 2, isoform F
NM_175963.3	NP_787977.2	chorion factor 2, isoform E
NM_001363925.1	NP_001350854.1	chorion factor 2, isoform G

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CF2 expression variations yield altered Mef2 expression levels but with correct spatio-temporal Mef2 expression patterns, it can be concluded that only the mechanism controlling expression levels is de-regulated. Here, it is proposed that CF2 regulates Mef2 expression through a Feedforward Loop circuit.
Title: CF2 transcription factor is involved in the regulation of Mef2 RNA levels, nuclei number and muscle fiber size.

These results indicate a role for CF2 as a direct negative regulator of the thin filament protein gene Actin 88F.
Title: CF2 represses Actin 88F gene expression and maintains filament balance during indirect flight muscle development in Drosophila.

CF2 depletion alters the relative expression of thin and thick filament components of muscle.
Title: CF2 activity and enhancer integration are required for proper muscle gene expression in Drosophila.

Mef2 and Cf2 collaborate in activation of the myogenic program in Drosophila.
Title: Myocyte enhancer factor 2 and chorion factor 2 collaborate in activation of the myogenic program in Drosophila.

CF2 expression during muscle development is dependent on the myogenic factor MEF2.
Title: The Drosophila zinc finger transcription factor CF2 is a myogenic marker downstream of MEF2 during muscle development.

The following Cf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa57981	NM_001363925.1 Latest version!	Drosophila melanogaster chorion factor 2, transcript variant G (Cf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OFa13774	NM_001273128.1 Latest version!	Drosophila melanogaster chorion factor 2, transcript variant F (Cf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa16212	NM_175963.3 Latest version!	Drosophila melanogaster chorion factor 2, transcript variant E (Cf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa16212	NM_175965.2 Latest version!	Drosophila melanogaster chorion factor 2, transcript variant C (Cf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa16212	NM_175964.2 Latest version!	Drosophila melanogaster chorion factor 2, transcript variant B (Cf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa16213	NM_078750.4 Latest version!	Drosophila melanogaster chorion factor 2, transcript variant A (Cf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cf2 ( NM_001363925.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001350854.1 Drosophila melanogaster chorion factor 2, transcript variant G (Cf2), mRNA.

Price & Availability↑

CloneID	OFa57981
Clone ID Related Accession (Same CDS sequence)	NM_001363925.1
Accession Version	NM_001363925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chorion factor 2, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATAAAGT CCACCACGAA TCCACAGGAA CAGCGTCTGC CACGCCCCGA GGATCAGTCG 
CCAGCGCCGC CGCCGCCGCC CCCCTCCTCA GCCACCACCT CCACCGCCGC CCCAGCCACG 
CCCACGCACC AAGTGGCCAC CGTGATAGCC AACATGGACA CCCTGAAGAC AGCCTTTCTG 
CCCAATCTCA GCATGGACCC CAATGTGCAC GTGTCGCCGC ACTATTGTCC CATGTGTCAC 
CAGCAGTTTG AGCGACCGCA GCACGTCGCG GATCACATGC AGCTGTGCCA CGGGATCACG 
CTGAACGCCC AGGGAGCCAT TGCCACGCTG GACGGCGGTC ATCCGCAGGC ACAGCAGCAC 
CCCAAGCTCA GCCACCCCTG CTTCAATTGT GATGAAAAGT TTGGCAACGC CGTCGATTTA 
GACGAACACC ATCGGCTGGC CCATCAGACG CCCGCCTTCC TGTCCCGCTG CCTTATGTGC 
AGCATCTATG GCATCCACTC GGCCACCCAG CAGCCCAACG AGTACAAATG CACGCAGTGC 
GGCTCCATTT GCACCACCGC CATGCTGGCT GCCGGACAGC AGGGCTTTAT GGAACAGCAG 
GAGGCGGCGG TGACGCCCGA CGATCAGCTG CCGGCAATGG CGCCCCGTGA TATGAGACTG 
ACGCCCGAGG AGCAGCACCA TCAGCAGCAA CTGCAGGCGG AGCACCACCA TCAGCAACAG 
CACCAACAAC AACAACAGCA GCAGCAGCAG CAGCAGGAAC TGCTGGAGCA GCAGCAGCGT 
GAAATGCAGG AGCAGGCGCA ACAGCAGCAG GTGCACCATC ACCAGCAGGA TCAGGATCTC 
GCCGGCGACC AGGTGGCGCT GAAGGTGCCA CCACTCACCG TCAAGCTAAA CAAGAACGCC 
AACGGTGGCG CCATTGTGTC GCATCCGCAG GTCATTATCA AGGAGGAGCC ACTCAGCCTG 
AGCGACAGTG GTGATGTTGT CAACTCTGTG CCTGTCTATG CCATACAAGC CAATCCCGGT 
GTACCCGCTC CGGCCAGTTC GGGTGTGCTA GTCGGCACAC AAACGGTACC TGCCGATCTG 
GCGCACAAGA TCCGGCACAA ATGTCCGGAT TGTCCAAAGA CCTTCAAGAC GCCCGGCACG 
CTGGCCATGC ACCGCAAGAT ACACACAGGC GAAGCAGACG CCACGCCCAA AGAACGCCCC 
TACACGTGCT CCTACTGCGG CAAGTCCTTC ACTCAATCGA ATACACTAAA ACAGCACACT 
CGCATACATA CAGGAGAGAA GCCGTTCCAC TGCGGTTACT GCGAGAAGTC CTTCAGCGTG 
AAGGACTATC TGACCAAGCA CATACGGACG CACACCGGCG AGAAGCCGTA CACCTGTCCG 
TATTGCGACA AGCGCTTCAC GCAGCGCAGC GCCCTCACTG TGCACACGAC CAAGCTGCAT 
CCGCTCTAGG GAAGGCCGGG CGGTGGCCGC CAGTTACCCG TTCCTGCCCC AGCCGCTCCT 
CCTCCTCCTA CTCATCCTCC AAATCCTTCT GGTCCTGGTG CGCCGCCGCC GTTGTCCGCC 
GACACGGATA CAGATCCCAA GAAGCCATCT GCGGCGGCTG CGGCATCGGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350854.1
CDS	344..1957
Translation	MIKSTTNPQEQRLPRPEDQSPAPPPPPPSSATTSTAAPATPTHQVATVIANMDTLKTAFLPNLSMDPNVHVSPHYCPMCHQQFERPQHVADHMQLCHGITLNAQGAIATLDGGHPQAQQHPKLSHPCFNCDEKFGNAVDLDEHHRLAHQTPAFLSRCLMCSIYGIHSATQQPNEYKCTQCGSICTTAMLAAGQQGFMEQQEAAVTPDDQLPAMAPRDMRLTPEEQHHQQQLQAEHHHQQQHQQQQQQQQQQQELLEQQQREMQEQAQQQQVHHHQQDQDLAGDQVALKVPPLTVKLNKNANGGAIVSHPQVIIKEEPLSLSDSGDVVNSVPVYAIQANPGVPAPASSGVLVGTQTVPADLAHKIRHKCPDCPKTFKTPGTLAMHRKIHTGEADATPKERPYTCSYCGKSFTQSNTLKQHTRIHTGEKPFHCGYCEKSFSVKDYLTKHIRTHTGEKPYTCPYCDKRFTQRSALTVHTTKLHPL*GRPGGGRQLPVPAPAAPPPPTHPPNPSGPGAPPPLSADTDTDPKKPSAAAAASA

Target ORF information:

RefSeq Version	NM_001363925.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chorion factor 2, transcript variant G (Cf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363925.1

ORF Insert Sequence:

ATGATAAAGT CCACCACGAA TCCACAGGAA CAGCGTCTGC CACGCCCCGA GGATCAGTCG 
CCAGCGCCGC CGCCGCCGCC CCCCTCCTCA GCCACCACCT CCACCGCCGC CCCAGCCACG 
CCCACGCACC AAGTGGCCAC CGTGATAGCC AACATGGACA CCCTGAAGAC AGCCTTTCTG 
CCCAATCTCA GCATGGACCC CAATGTGCAC GTGTCGCCGC ACTATTGTCC CATGTGTCAC 
CAGCAGTTTG AGCGACCGCA GCACGTCGCG GATCACATGC AGCTGTGCCA CGGGATCACG 
CTGAACGCCC AGGGAGCCAT TGCCACGCTG GACGGCGGTC ATCCGCAGGC ACAGCAGCAC 
CCCAAGCTCA GCCACCCCTG CTTCAATTGT GATGAAAAGT TTGGCAACGC CGTCGATTTA 
GACGAACACC ATCGGCTGGC CCATCAGACG CCCGCCTTCC TGTCCCGCTG CCTTATGTGC 
AGCATCTATG GCATCCACTC GGCCACCCAG CAGCCCAACG AGTACAAATG CACGCAGTGC 
GGCTCCATTT GCACCACCGC CATGCTGGCT GCCGGACAGC AGGGCTTTAT GGAACAGCAG 
GAGGCGGCGG TGACGCCCGA CGATCAGCTG CCGGCAATGG CGCCCCGTGA TATGAGACTG 
ACGCCCGAGG AGCAGCACCA TCAGCAGCAA CTGCAGGCGG AGCACCACCA TCAGCAACAG 
CACCAACAAC AACAACAGCA GCAGCAGCAG CAGCAGGAAC TGCTGGAGCA GCAGCAGCGT 
GAAATGCAGG AGCAGGCGCA ACAGCAGCAG GTGCACCATC ACCAGCAGGA TCAGGATCTC 
GCCGGCGACC AGGTGGCGCT GAAGGTGCCA CCACTCACCG TCAAGCTAAA CAAGAACGCC 
AACGGTGGCG CCATTGTGTC GCATCCGCAG GTCATTATCA AGGAGGAGCC ACTCAGCCTG 
AGCGACAGTG GTGATGTTGT CAACTCTGTG CCTGTCTATG CCATACAAGC CAATCCCGGT 
GTACCCGCTC CGGCCAGTTC GGGTGTGCTA GTCGGCACAC AAACGGTACC TGCCGATCTG 
GCGCACAAGA TCCGGCACAA ATGTCCGGAT TGTCCAAAGA CCTTCAAGAC GCCCGGCACG 
CTGGCCATGC ACCGCAAGAT ACACACAGGC GAAGCAGACG CCACGCCCAA AGAACGCCCC 
TACACGTGCT CCTACTGCGG CAAGTCCTTC ACTCAATCGA ATACACTAAA ACAGCACACT 
CGCATACATA CAGGAGAGAA GCCGTTCCAC TGCGGTTACT GCGAGAAGTC CTTCAGCGTG 
AAGGACTATC TGACCAAGCA CATACGGACG CACACCGGCG AGAAGCCGTA CACCTGTCCG 
TATTGCGACA AGCGCTTCAC GCAGCGCAGC GCCCTCACTG TGCACACGAC CAAGCTGCAT 
CCGCTCTAGG GAAGGCCGGG CGGTGGCCGC CAGTTACCCG TTCCTGCCCC AGCCGCTCCT 
CCTCCTCCTA CTCATCCTCC AAATCCTTCT GGTCCTGGTG CGCCGCCGCC GTTGTCCGCC 
GACACGGATA CAGATCCCAA GAAGCCATCT GCGGCGGCTG CGGCATCGGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cf2 ( NM_001273128.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260057.1 Drosophila melanogaster chorion factor 2, transcript variant F (Cf2), mRNA.

Price & Availability↑

CloneID	OFa13774
Clone ID Related Accession (Same CDS sequence)	NM_001273128.1
Accession Version	NM_001273128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chorion factor 2, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACACCC TGAAGACAGC CTTTCTGCCC AATCTCAGCA TGGACCCCAA TGTGCACGTG 
TCGCCGCACT ATTGTCCCAT GTGTCACCAG CAGTTTGAGC GACCGCAGCA CGTCGCGGAT 
CACATGCAGC TGTGCCACGG GATCACGCTG AACGCCCAGG GAGCCATTGC CACGCTGGAC 
GGCGGTCATC CGCAGGCACA GCAGCACCCC AAGCTCAGCC ACCCCTGCTT CAATTGTGAT 
GAAAAGTTTG GCAACGCCGT CGATTTAGAC GAACACCATC GGCTGGCCCA TCAGACGCCC 
GCCTTCCTGT CCCGCTGCCT TATGTGCAGC ATCTATGGCA TCCACTCGGC CACCCAGCAG 
CCCAACGAGT ACAAATGCAC GCAGTGCGGC TCCATTTGCA CCACCGCCAT GCTGGCTGCC 
GGACAGCAGG GCTTTATGGA ACAGCAGGAG GCGGCGGTGA CGCCCGACGA TCAGCTGCCG 
GCAATGGCGC CCCGTGATAT GAGACTGACG CCCGAGGAGC AGCACCATCA GCAGCAACTG 
CAGGCGGAGC ACCACCATCA GCAACAGCAC CAACAACAAC AACAGCAGCA GCAGCAGCAG 
CAGGAACTGC TGGAGCAGCA GCAGCGTGAA ATGCAGGAGC AGGCGCAACA GCAGCAGGTG 
CACCATCACC AGCAGGATCA GGATCTCGCC GGCGACCAGG TGGCGCTGAA GGTGCCACCA 
CTCACCGTCA AGCTAAACAA GAACGCCAAC GGTGGCGCCA TTGTGTCGCA TCCGCAGGTC 
ATTATCAAGG AGGAGCCACT CAGCCTGAGC GACAGTGGTG ATGTTGTCAA CTCTGTGCCT 
GTCTATGCCA TACAAGCCAA TCCCGGTGTA CCCGCTCCGG CCAGTTCGGG TGTGCTAGTC 
GGCACACAAA CGGTACCTGC CGATCTGGCG CACAAGATCC GGCACAAATG TCCGGATTGT 
CCAAAGACCT TCAAGACGCC CGGCACGCTG GCCATGCACC GCAAGATACA CACAGGCGAA 
GCAGACGCCA CGCCCAAAGA ACGCCCCTAC ACGTGCTCCT ACTGCGGCAA GTCCTTCACT 
CAATCGAATA CACTAAAACA GCACACTCGC ATACATACAG GAGAGAAGCC GTTCCACTGC 
GGTTACTGCG AGAAGTCCTT CAGCGTGAAG GACTATCTGA CCAAGCACAT ACGGACGCAC 
ACCGGCGAGA AGCCGTACAC CTGTCCGTAT TGCGACAAGC GCTTCACGCA GCGCAGCGCC 
CTCACTGTGC ACACGACCAA GCTGCATCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260057.1
CDS	370..1665
Translation	MDTLKTAFLPNLSMDPNVHVSPHYCPMCHQQFERPQHVADHMQLCHGITLNAQGAIATLDGGHPQAQQHPKLSHPCFNCDEKFGNAVDLDEHHRLAHQTPAFLSRCLMCSIYGIHSATQQPNEYKCTQCGSICTTAMLAAGQQGFMEQQEAAVTPDDQLPAMAPRDMRLTPEEQHHQQQLQAEHHHQQQHQQQQQQQQQQQELLEQQQREMQEQAQQQQVHHHQQDQDLAGDQVALKVPPLTVKLNKNANGGAIVSHPQVIIKEEPLSLSDSGDVVNSVPVYAIQANPGVPAPASSGVLVGTQTVPADLAHKIRHKCPDCPKTFKTPGTLAMHRKIHTGEADATPKERPYTCSYCGKSFTQSNTLKQHTRIHTGEKPFHCGYCEKSFSVKDYLTKHIRTHTGEKPYTCPYCDKRFTQRSALTVHTTKLHPL

Target ORF information:

RefSeq Version	NM_001273128.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chorion factor 2, transcript variant F (Cf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273128.1

ORF Insert Sequence:

ATGGACACCC TGAAGACAGC CTTTCTGCCC AATCTCAGCA TGGACCCCAA TGTGCACGTG 
TCGCCGCACT ATTGTCCCAT GTGTCACCAG CAGTTTGAGC GACCGCAGCA CGTCGCGGAT 
CACATGCAGC TGTGCCACGG GATCACGCTG AACGCCCAGG GAGCCATTGC CACGCTGGAC 
GGCGGTCATC CGCAGGCACA GCAGCACCCC AAGCTCAGCC ACCCCTGCTT CAATTGTGAT 
GAAAAGTTTG GCAACGCCGT CGATTTAGAC GAACACCATC GGCTGGCCCA TCAGACGCCC 
GCCTTCCTGT CCCGCTGCCT TATGTGCAGC ATCTATGGCA TCCACTCGGC CACCCAGCAG 
CCCAACGAGT ACAAATGCAC GCAGTGCGGC TCCATTTGCA CCACCGCCAT GCTGGCTGCC 
GGACAGCAGG GCTTTATGGA ACAGCAGGAG GCGGCGGTGA CGCCCGACGA TCAGCTGCCG 
GCAATGGCGC CCCGTGATAT GAGACTGACG CCCGAGGAGC AGCACCATCA GCAGCAACTG 
CAGGCGGAGC ACCACCATCA GCAACAGCAC CAACAACAAC AACAGCAGCA GCAGCAGCAG 
CAGGAACTGC TGGAGCAGCA GCAGCGTGAA ATGCAGGAGC AGGCGCAACA GCAGCAGGTG 
CACCATCACC AGCAGGATCA GGATCTCGCC GGCGACCAGG TGGCGCTGAA GGTGCCACCA 
CTCACCGTCA AGCTAAACAA GAACGCCAAC GGTGGCGCCA TTGTGTCGCA TCCGCAGGTC 
ATTATCAAGG AGGAGCCACT CAGCCTGAGC GACAGTGGTG ATGTTGTCAA CTCTGTGCCT 
GTCTATGCCA TACAAGCCAA TCCCGGTGTA CCCGCTCCGG CCAGTTCGGG TGTGCTAGTC 
GGCACACAAA CGGTACCTGC CGATCTGGCG CACAAGATCC GGCACAAATG TCCGGATTGT 
CCAAAGACCT TCAAGACGCC CGGCACGCTG GCCATGCACC GCAAGATACA CACAGGCGAA 
GCAGACGCCA CGCCCAAAGA ACGCCCCTAC ACGTGCTCCT ACTGCGGCAA GTCCTTCACT 
CAATCGAATA CACTAAAACA GCACACTCGC ATACATACAG GAGAGAAGCC GTTCCACTGC 
GGTTACTGCG AGAAGTCCTT CAGCGTGAAG GACTATCTGA CCAAGCACAT ACGGACGCAC 
ACCGGCGAGA AGCCGTACAC CTGTCCGTAT TGCGACAAGC GCTTCACGCA GCGCAGCGCC 
CTCACTGTGC ACACGACCAA GCTGCATCCG CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cf2 ( NM_175965.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_787979.1 Drosophila melanogaster chorion factor 2, transcript variant C (Cf2), mRNA.

Price & Availability↑

CloneID	OFa16212
Clone ID Related Accession (Same CDS sequence)	NM_175965.2 , NM_175964.2 , NM_175963.3
Accession Version	NM_175965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chorion factor 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_175965.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATAAAGT CCACCACGAA TCCACAGGAA CAGCGTCTGC CACGCCCCGA GGATCAGTCG 
CCAGCGCCGC CGCCGCCGCC CCCCTCCTCA GCCACCACCT CCACCGCCGC CCCAGCCACG 
CCCACGCACC AAGTGGCCAC CGTGATAGCC AACATGGACA CCCTGAAGAC AGCCTTTCTG 
CCCAATCTCA GCATGGACCC CAATGTGCAC GTGTCGCCGC ACTATTGTCC CATGTGTCAC 
CAGCAGTTTG AGCGACCGCA GCACGTCGCG GATCACATGC AGCTGTGCCA CGGGATCACG 
CTGAACGCCC AGGGAGCCAT TGCCACGCTG GACGGCGGTC ATCCGCAGGC ACAGCAGCAC 
CCCAAGCTCA GCCACCCCTG CTTCAATTGT GATGAAAAGT TTGGCAACGC CGTCGATTTA 
GACGAACACC ATCGGCTGGC CCATCAGACG CCCGCCTTCC TGTCCCGCTG CCTTATGTGC 
AGCATCTATG GCATCCACTC GGCCACCCAG CAGCCCAACG AGTACAAATG CACGCAGTGC 
GGCTCCATTT GCACCACCGC CATGCTGGCT GCCGGACAGC AGGGCTTTAT GGAACAGCAG 
GAGGCGGCGG TGACGCCCGA CGATCAGCTG CCGGCAATGG CGCCCCGTGA TATGAGACTG 
ACGCCCGAGG AGCAGCACCA TCAGCAGCAA CTGCAGGCGG AGCACCACCA TCAGCAACAG 
CACCAACAAC AACAACAGCA GCAGCAGCAG CAGCAGGAAC TGCTGGAGCA GCAGCAGCGT 
GAAATGCAGG AGCAGGCGCA ACAGCAGCAG GTGCACCATC ACCAGCAGGA TCAGGATCTC 
GCCGGCGACC AGGTGGCGCT GAAGGTGCCA CCACTCACCG TCAAGCTAAA CAAGAACGCC 
AACGGTGGCG CCATTGTGTC GCATCCGCAG GTCATTATCA AGGAGGAGCC ACTCAGCCTG 
AGCGACAGTG GTGATGTTGT CAACTCTGTG CCTGTCTATG CCATACAAGC CAATCCCGGT 
GTACCCGCTC CGGCCAGTTC GGGTGTGCTA GTCGGCACAC AAACGGTACC TGCCGATCTG 
GCGCACAAGA TCCGGCACAA ATGTCCGGAT TGTCCAAAGA CCTTCAAGAC GCCCGGCACG 
CTGGCCATGC ACCGCAAGAT ACACACAGGC GAAGCAGACG CCACGCCCAA AGAACGCCCC 
TACACGTGCT CCTACTGCGG CAAGTCCTTC ACTCAATCGA ATACACTAAA ACAGCACACT 
CGCATACATA CAGGAGAGAA GCCGTTCCAC TGCGGTTACT GCGAGAAGTC CTTCAGCGTG 
AAGGACTATC TGACCAAGCA CATACGGACG CACACCGGCG AGAAGCCGTA CACCTGTCCG 
TATTGCGACA AGCGCTTCAC GCAGCGCAGC GCCCTCACTG TGCACACGAC CAAGCTGCAT 
CCGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787979.1
CDS	344..1792
Translation	MIKSTTNPQEQRLPRPEDQSPAPPPPPPSSATTSTAAPATPTHQVATVIANMDTLKTAFLPNLSMDPNVHVSPHYCPMCHQQFERPQHVADHMQLCHGITLNAQGAIATLDGGHPQAQQHPKLSHPCFNCDEKFGNAVDLDEHHRLAHQTPAFLSRCLMCSIYGIHSATQQPNEYKCTQCGSICTTAMLAAGQQGFMEQQEAAVTPDDQLPAMAPRDMRLTPEEQHHQQQLQAEHHHQQQHQQQQQQQQQQQELLEQQQREMQEQAQQQQVHHHQQDQDLAGDQVALKVPPLTVKLNKNANGGAIVSHPQVIIKEEPLSLSDSGDVVNSVPVYAIQANPGVPAPASSGVLVGTQTVPADLAHKIRHKCPDCPKTFKTPGTLAMHRKIHTGEADATPKERPYTCSYCGKSFTQSNTLKQHTRIHTGEKPFHCGYCEKSFSVKDYLTKHIRTHTGEKPYTCPYCDKRFTQRSALTVHTTKLHPL

Target ORF information:

RefSeq Version	NM_175965.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chorion factor 2, transcript variant C (Cf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175965.2

ORF Insert Sequence:

ATGATAAAGT CCACCACGAA TCCACAGGAA CAGCGTCTGC CACGCCCCGA GGATCAGTCG 
CCAGCGCCGC CGCCGCCGCC CCCCTCCTCA GCCACCACCT CCACCGCCGC CCCAGCCACG 
CCCACGCACC AAGTGGCCAC CGTGATAGCC AACATGGACA CCCTGAAGAC AGCCTTTCTG 
CCCAATCTCA GCATGGACCC CAATGTGCAC GTGTCGCCGC ACTATTGTCC CATGTGTCAC 
CAGCAGTTTG AGCGACCGCA GCACGTCGCG GATCACATGC AGCTGTGCCA CGGGATCACG 
CTGAACGCCC AGGGAGCCAT TGCCACGCTG GACGGCGGTC ATCCGCAGGC ACAGCAGCAC 
CCCAAGCTCA GCCACCCCTG CTTCAATTGT GATGAAAAGT TTGGCAACGC CGTCGATTTA 
GACGAACACC ATCGGCTGGC CCATCAGACG CCCGCCTTCC TGTCCCGCTG CCTTATGTGC 
AGCATCTATG GCATCCACTC GGCCACCCAG CAGCCCAACG AGTACAAATG CACGCAGTGC 
GGCTCCATTT GCACCACCGC CATGCTGGCT GCCGGACAGC AGGGCTTTAT GGAACAGCAG 
GAGGCGGCGG TGACGCCCGA CGATCAGCTG CCGGCAATGG CGCCCCGTGA TATGAGACTG 
ACGCCCGAGG AGCAGCACCA TCAGCAGCAA CTGCAGGCGG AGCACCACCA TCAGCAACAG 
CACCAACAAC AACAACAGCA GCAGCAGCAG CAGCAGGAAC TGCTGGAGCA GCAGCAGCGT 
GAAATGCAGG AGCAGGCGCA ACAGCAGCAG GTGCACCATC ACCAGCAGGA TCAGGATCTC 
GCCGGCGACC AGGTGGCGCT GAAGGTGCCA CCACTCACCG TCAAGCTAAA CAAGAACGCC 
AACGGTGGCG CCATTGTGTC GCATCCGCAG GTCATTATCA AGGAGGAGCC ACTCAGCCTG 
AGCGACAGTG GTGATGTTGT CAACTCTGTG CCTGTCTATG CCATACAAGC CAATCCCGGT 
GTACCCGCTC CGGCCAGTTC GGGTGTGCTA GTCGGCACAC AAACGGTACC TGCCGATCTG 
GCGCACAAGA TCCGGCACAA ATGTCCGGAT TGTCCAAAGA CCTTCAAGAC GCCCGGCACG 
CTGGCCATGC ACCGCAAGAT ACACACAGGC GAAGCAGACG CCACGCCCAA AGAACGCCCC 
TACACGTGCT CCTACTGCGG CAAGTCCTTC ACTCAATCGA ATACACTAAA ACAGCACACT 
CGCATACATA CAGGAGAGAA GCCGTTCCAC TGCGGTTACT GCGAGAAGTC CTTCAGCGTG 
AAGGACTATC TGACCAAGCA CATACGGACG CACACCGGCG AGAAGCCGTA CACCTGTCCG 
TATTGCGACA AGCGCTTCAC GCAGCGCAGC GCCCTCACTG TGCACACGAC CAAGCTGCAT 
CCGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cf2 ( NM_175964.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_787978.1 Drosophila melanogaster chorion factor 2, transcript variant B (Cf2), mRNA.

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CloneID	OFa16212
Clone ID Related Accession (Same CDS sequence)	NM_175965.2 , NM_175964.2 , NM_175963.3
Accession Version	NM_175964.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chorion factor 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_175964.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATAAAGT CCACCACGAA TCCACAGGAA CAGCGTCTGC CACGCCCCGA GGATCAGTCG 
CCAGCGCCGC CGCCGCCGCC CCCCTCCTCA GCCACCACCT CCACCGCCGC CCCAGCCACG 
CCCACGCACC AAGTGGCCAC CGTGATAGCC AACATGGACA CCCTGAAGAC AGCCTTTCTG 
CCCAATCTCA GCATGGACCC CAATGTGCAC GTGTCGCCGC ACTATTGTCC CATGTGTCAC 
CAGCAGTTTG AGCGACCGCA GCACGTCGCG GATCACATGC AGCTGTGCCA CGGGATCACG 
CTGAACGCCC AGGGAGCCAT TGCCACGCTG GACGGCGGTC ATCCGCAGGC ACAGCAGCAC 
CCCAAGCTCA GCCACCCCTG CTTCAATTGT GATGAAAAGT TTGGCAACGC CGTCGATTTA 
GACGAACACC ATCGGCTGGC CCATCAGACG CCCGCCTTCC TGTCCCGCTG CCTTATGTGC 
AGCATCTATG GCATCCACTC GGCCACCCAG CAGCCCAACG AGTACAAATG CACGCAGTGC 
GGCTCCATTT GCACCACCGC CATGCTGGCT GCCGGACAGC AGGGCTTTAT GGAACAGCAG 
GAGGCGGCGG TGACGCCCGA CGATCAGCTG CCGGCAATGG CGCCCCGTGA TATGAGACTG 
ACGCCCGAGG AGCAGCACCA TCAGCAGCAA CTGCAGGCGG AGCACCACCA TCAGCAACAG 
CACCAACAAC AACAACAGCA GCAGCAGCAG CAGCAGGAAC TGCTGGAGCA GCAGCAGCGT 
GAAATGCAGG AGCAGGCGCA ACAGCAGCAG GTGCACCATC ACCAGCAGGA TCAGGATCTC 
GCCGGCGACC AGGTGGCGCT GAAGGTGCCA CCACTCACCG TCAAGCTAAA CAAGAACGCC 
AACGGTGGCG CCATTGTGTC GCATCCGCAG GTCATTATCA AGGAGGAGCC ACTCAGCCTG 
AGCGACAGTG GTGATGTTGT CAACTCTGTG CCTGTCTATG CCATACAAGC CAATCCCGGT 
GTACCCGCTC CGGCCAGTTC GGGTGTGCTA GTCGGCACAC AAACGGTACC TGCCGATCTG 
GCGCACAAGA TCCGGCACAA ATGTCCGGAT TGTCCAAAGA CCTTCAAGAC GCCCGGCACG 
CTGGCCATGC ACCGCAAGAT ACACACAGGC GAAGCAGACG CCACGCCCAA AGAACGCCCC 
TACACGTGCT CCTACTGCGG CAAGTCCTTC ACTCAATCGA ATACACTAAA ACAGCACACT 
CGCATACATA CAGGAGAGAA GCCGTTCCAC TGCGGTTACT GCGAGAAGTC CTTCAGCGTG 
AAGGACTATC TGACCAAGCA CATACGGACG CACACCGGCG AGAAGCCGTA CACCTGTCCG 
TATTGCGACA AGCGCTTCAC GCAGCGCAGC GCCCTCACTG TGCACACGAC CAAGCTGCAT 
CCGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787978.1
CDS	320..1768
Translation	MIKSTTNPQEQRLPRPEDQSPAPPPPPPSSATTSTAAPATPTHQVATVIANMDTLKTAFLPNLSMDPNVHVSPHYCPMCHQQFERPQHVADHMQLCHGITLNAQGAIATLDGGHPQAQQHPKLSHPCFNCDEKFGNAVDLDEHHRLAHQTPAFLSRCLMCSIYGIHSATQQPNEYKCTQCGSICTTAMLAAGQQGFMEQQEAAVTPDDQLPAMAPRDMRLTPEEQHHQQQLQAEHHHQQQHQQQQQQQQQQQELLEQQQREMQEQAQQQQVHHHQQDQDLAGDQVALKVPPLTVKLNKNANGGAIVSHPQVIIKEEPLSLSDSGDVVNSVPVYAIQANPGVPAPASSGVLVGTQTVPADLAHKIRHKCPDCPKTFKTPGTLAMHRKIHTGEADATPKERPYTCSYCGKSFTQSNTLKQHTRIHTGEKPFHCGYCEKSFSVKDYLTKHIRTHTGEKPYTCPYCDKRFTQRSALTVHTTKLHPL

Target ORF information:

RefSeq Version	NM_175964.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chorion factor 2, transcript variant B (Cf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175964.2

ORF Insert Sequence:

ATGATAAAGT CCACCACGAA TCCACAGGAA CAGCGTCTGC CACGCCCCGA GGATCAGTCG 
CCAGCGCCGC CGCCGCCGCC CCCCTCCTCA GCCACCACCT CCACCGCCGC CCCAGCCACG 
CCCACGCACC AAGTGGCCAC CGTGATAGCC AACATGGACA CCCTGAAGAC AGCCTTTCTG 
CCCAATCTCA GCATGGACCC CAATGTGCAC GTGTCGCCGC ACTATTGTCC CATGTGTCAC 
CAGCAGTTTG AGCGACCGCA GCACGTCGCG GATCACATGC AGCTGTGCCA CGGGATCACG 
CTGAACGCCC AGGGAGCCAT TGCCACGCTG GACGGCGGTC ATCCGCAGGC ACAGCAGCAC 
CCCAAGCTCA GCCACCCCTG CTTCAATTGT GATGAAAAGT TTGGCAACGC CGTCGATTTA 
GACGAACACC ATCGGCTGGC CCATCAGACG CCCGCCTTCC TGTCCCGCTG CCTTATGTGC 
AGCATCTATG GCATCCACTC GGCCACCCAG CAGCCCAACG AGTACAAATG CACGCAGTGC 
GGCTCCATTT GCACCACCGC CATGCTGGCT GCCGGACAGC AGGGCTTTAT GGAACAGCAG 
GAGGCGGCGG TGACGCCCGA CGATCAGCTG CCGGCAATGG CGCCCCGTGA TATGAGACTG 
ACGCCCGAGG AGCAGCACCA TCAGCAGCAA CTGCAGGCGG AGCACCACCA TCAGCAACAG 
CACCAACAAC AACAACAGCA GCAGCAGCAG CAGCAGGAAC TGCTGGAGCA GCAGCAGCGT 
GAAATGCAGG AGCAGGCGCA ACAGCAGCAG GTGCACCATC ACCAGCAGGA TCAGGATCTC 
GCCGGCGACC AGGTGGCGCT GAAGGTGCCA CCACTCACCG TCAAGCTAAA CAAGAACGCC 
AACGGTGGCG CCATTGTGTC GCATCCGCAG GTCATTATCA AGGAGGAGCC ACTCAGCCTG 
AGCGACAGTG GTGATGTTGT CAACTCTGTG CCTGTCTATG CCATACAAGC CAATCCCGGT 
GTACCCGCTC CGGCCAGTTC GGGTGTGCTA GTCGGCACAC AAACGGTACC TGCCGATCTG 
GCGCACAAGA TCCGGCACAA ATGTCCGGAT TGTCCAAAGA CCTTCAAGAC GCCCGGCACG 
CTGGCCATGC ACCGCAAGAT ACACACAGGC GAAGCAGACG CCACGCCCAA AGAACGCCCC 
TACACGTGCT CCTACTGCGG CAAGTCCTTC ACTCAATCGA ATACACTAAA ACAGCACACT 
CGCATACATA CAGGAGAGAA GCCGTTCCAC TGCGGTTACT GCGAGAAGTC CTTCAGCGTG 
AAGGACTATC TGACCAAGCA CATACGGACG CACACCGGCG AGAAGCCGTA CACCTGTCCG 
TATTGCGACA AGCGCTTCAC GCAGCGCAGC GCCCTCACTG TGCACACGAC CAAGCTGCAT 
CCGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cf2 ( NM_175963.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_787977.2 Drosophila melanogaster chorion factor 2, transcript variant E (Cf2), mRNA.

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CloneID	OFa16212
Clone ID Related Accession (Same CDS sequence)	NM_175965.2 , NM_175964.2 , NM_175963.3
Accession Version	NM_175963.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chorion factor 2, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_175963.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATAAAGT CCACCACGAA TCCACAGGAA CAGCGTCTGC CACGCCCCGA GGATCAGTCG 
CCAGCGCCGC CGCCGCCGCC CCCCTCCTCA GCCACCACCT CCACCGCCGC CCCAGCCACG 
CCCACGCACC AAGTGGCCAC CGTGATAGCC AACATGGACA CCCTGAAGAC AGCCTTTCTG 
CCCAATCTCA GCATGGACCC CAATGTGCAC GTGTCGCCGC ACTATTGTCC CATGTGTCAC 
CAGCAGTTTG AGCGACCGCA GCACGTCGCG GATCACATGC AGCTGTGCCA CGGGATCACG 
CTGAACGCCC AGGGAGCCAT TGCCACGCTG GACGGCGGTC ATCCGCAGGC ACAGCAGCAC 
CCCAAGCTCA GCCACCCCTG CTTCAATTGT GATGAAAAGT TTGGCAACGC CGTCGATTTA 
GACGAACACC ATCGGCTGGC CCATCAGACG CCCGCCTTCC TGTCCCGCTG CCTTATGTGC 
AGCATCTATG GCATCCACTC GGCCACCCAG CAGCCCAACG AGTACAAATG CACGCAGTGC 
GGCTCCATTT GCACCACCGC CATGCTGGCT GCCGGACAGC AGGGCTTTAT GGAACAGCAG 
GAGGCGGCGG TGACGCCCGA CGATCAGCTG CCGGCAATGG CGCCCCGTGA TATGAGACTG 
ACGCCCGAGG AGCAGCACCA TCAGCAGCAA CTGCAGGCGG AGCACCACCA TCAGCAACAG 
CACCAACAAC AACAACAGCA GCAGCAGCAG CAGCAGGAAC TGCTGGAGCA GCAGCAGCGT 
GAAATGCAGG AGCAGGCGCA ACAGCAGCAG GTGCACCATC ACCAGCAGGA TCAGGATCTC 
GCCGGCGACC AGGTGGCGCT GAAGGTGCCA CCACTCACCG TCAAGCTAAA CAAGAACGCC 
AACGGTGGCG CCATTGTGTC GCATCCGCAG GTCATTATCA AGGAGGAGCC ACTCAGCCTG 
AGCGACAGTG GTGATGTTGT CAACTCTGTG CCTGTCTATG CCATACAAGC CAATCCCGGT 
GTACCCGCTC CGGCCAGTTC GGGTGTGCTA GTCGGCACAC AAACGGTACC TGCCGATCTG 
GCGCACAAGA TCCGGCACAA ATGTCCGGAT TGTCCAAAGA CCTTCAAGAC GCCCGGCACG 
CTGGCCATGC ACCGCAAGAT ACACACAGGC GAAGCAGACG CCACGCCCAA AGAACGCCCC 
TACACGTGCT CCTACTGCGG CAAGTCCTTC ACTCAATCGA ATACACTAAA ACAGCACACT 
CGCATACATA CAGGAGAGAA GCCGTTCCAC TGCGGTTACT GCGAGAAGTC CTTCAGCGTG 
AAGGACTATC TGACCAAGCA CATACGGACG CACACCGGCG AGAAGCCGTA CACCTGTCCG 
TATTGCGACA AGCGCTTCAC GCAGCGCAGC GCCCTCACTG TGCACACGAC CAAGCTGCAT 
CCGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787977.2
CDS	653..2101
Translation	MIKSTTNPQEQRLPRPEDQSPAPPPPPPSSATTSTAAPATPTHQVATVIANMDTLKTAFLPNLSMDPNVHVSPHYCPMCHQQFERPQHVADHMQLCHGITLNAQGAIATLDGGHPQAQQHPKLSHPCFNCDEKFGNAVDLDEHHRLAHQTPAFLSRCLMCSIYGIHSATQQPNEYKCTQCGSICTTAMLAAGQQGFMEQQEAAVTPDDQLPAMAPRDMRLTPEEQHHQQQLQAEHHHQQQHQQQQQQQQQQQELLEQQQREMQEQAQQQQVHHHQQDQDLAGDQVALKVPPLTVKLNKNANGGAIVSHPQVIIKEEPLSLSDSGDVVNSVPVYAIQANPGVPAPASSGVLVGTQTVPADLAHKIRHKCPDCPKTFKTPGTLAMHRKIHTGEADATPKERPYTCSYCGKSFTQSNTLKQHTRIHTGEKPFHCGYCEKSFSVKDYLTKHIRTHTGEKPYTCPYCDKRFTQRSALTVHTTKLHPL

Target ORF information:

RefSeq Version	NM_175963.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chorion factor 2, transcript variant E (Cf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175963.3

ORF Insert Sequence:

ATGATAAAGT CCACCACGAA TCCACAGGAA CAGCGTCTGC CACGCCCCGA GGATCAGTCG 
CCAGCGCCGC CGCCGCCGCC CCCCTCCTCA GCCACCACCT CCACCGCCGC CCCAGCCACG 
CCCACGCACC AAGTGGCCAC CGTGATAGCC AACATGGACA CCCTGAAGAC AGCCTTTCTG 
CCCAATCTCA GCATGGACCC CAATGTGCAC GTGTCGCCGC ACTATTGTCC CATGTGTCAC 
CAGCAGTTTG AGCGACCGCA GCACGTCGCG GATCACATGC AGCTGTGCCA CGGGATCACG 
CTGAACGCCC AGGGAGCCAT TGCCACGCTG GACGGCGGTC ATCCGCAGGC ACAGCAGCAC 
CCCAAGCTCA GCCACCCCTG CTTCAATTGT GATGAAAAGT TTGGCAACGC CGTCGATTTA 
GACGAACACC ATCGGCTGGC CCATCAGACG CCCGCCTTCC TGTCCCGCTG CCTTATGTGC 
AGCATCTATG GCATCCACTC GGCCACCCAG CAGCCCAACG AGTACAAATG CACGCAGTGC 
GGCTCCATTT GCACCACCGC CATGCTGGCT GCCGGACAGC AGGGCTTTAT GGAACAGCAG 
GAGGCGGCGG TGACGCCCGA CGATCAGCTG CCGGCAATGG CGCCCCGTGA TATGAGACTG 
ACGCCCGAGG AGCAGCACCA TCAGCAGCAA CTGCAGGCGG AGCACCACCA TCAGCAACAG 
CACCAACAAC AACAACAGCA GCAGCAGCAG CAGCAGGAAC TGCTGGAGCA GCAGCAGCGT 
GAAATGCAGG AGCAGGCGCA ACAGCAGCAG GTGCACCATC ACCAGCAGGA TCAGGATCTC 
GCCGGCGACC AGGTGGCGCT GAAGGTGCCA CCACTCACCG TCAAGCTAAA CAAGAACGCC 
AACGGTGGCG CCATTGTGTC GCATCCGCAG GTCATTATCA AGGAGGAGCC ACTCAGCCTG 
AGCGACAGTG GTGATGTTGT CAACTCTGTG CCTGTCTATG CCATACAAGC CAATCCCGGT 
GTACCCGCTC CGGCCAGTTC GGGTGTGCTA GTCGGCACAC AAACGGTACC TGCCGATCTG 
GCGCACAAGA TCCGGCACAA ATGTCCGGAT TGTCCAAAGA CCTTCAAGAC GCCCGGCACG 
CTGGCCATGC ACCGCAAGAT ACACACAGGC GAAGCAGACG CCACGCCCAA AGAACGCCCC 
TACACGTGCT CCTACTGCGG CAAGTCCTTC ACTCAATCGA ATACACTAAA ACAGCACACT 
CGCATACATA CAGGAGAGAA GCCGTTCCAC TGCGGTTACT GCGAGAAGTC CTTCAGCGTG 
AAGGACTATC TGACCAAGCA CATACGGACG CACACCGGCG AGAAGCCGTA CACCTGTCCG 
TATTGCGACA AGCGCTTCAC GCAGCGCAGC GCCCTCACTG TGCACACGAC CAAGCTGCAT 
CCGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cf2 ( NM_078750.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523474.1 Drosophila melanogaster chorion factor 2, transcript variant A (Cf2), mRNA.

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CloneID	OFa16213
Clone ID Related Accession (Same CDS sequence)	NM_078750.4
Accession Version	NM_078750.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chorion factor 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078750.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATAAAGT CCACCACGAA TCCACAGGAA CAGCGTCTGC CACGCCCCGA GGATCAGTCG 
CCAGCGCCGC CGCCGCCGCC CCCCTCCTCA GCCACCACCT CCACCGCCGC CCCAGCCACG 
CCCACGCACC AAGTGGCCAC CGTGATAGCC AACATGGACA CCCTGAAGAC AGCCTTTCTG 
CCCAATCTCA GCATGGACCC CAATGTGCAC GTGTCGCCGC ACTATTGTCC CATGTGTCAC 
CAGCAGTTTG AGCGACCGCA GCACGTCGCG GATCACATGC AGCTGTGCCA CGGGATCACG 
CTGAACGCCC AGGGAGCCAT TGCCACGCTG GACGGCGGTC ATCCGCAGGC ACAGCAGCAC 
CCCAAGCTCA GCCACCCCTG CTTCAATTGT GATGAAAAGT TTGGCAACGC CGTCGATTTA 
GACGAACACC ATCGGCTGGC CCATCAGACG CCCGCCTTCC TGTCCCGCTG CCTTATGTGC 
AGCATCTATG GCATCCACTC GGCCACCCAG CAGCCCAACG AGTACAAATG CACGCAGTGC 
GGCTCCATTT GCACCACCGC CATGCTGGCT GCCGGACAGC AGGGCTTTAT GGAACAGCAG 
GAGGCGGCGG TGACGCCCGA CGATCAGCTG CCGGCAATGG CGCCCCGTGA TATGAGACTG 
ACGCCCGAGG AGCAGCACCA TCAGCAGCAA CTGCAGGCGG AGCACCACCA TCAGCAACAG 
CACCAACAAC AACAACAGCA GCAGCAGCAG CAGCAGGAAC TGCTGGAGCA GCAGCAGCGT 
GAAATGCAGG AGCAGGCGCA ACAGCAGCAG GTGCACCATC ACCAGCAGGA TCAGGATCTC 
GCCGGCGACC AGGTGGCGCT GAAGGTGCCA CCACTCACCG TCAAGCTAAA CAAGAACGCC 
AACGGTGGCG CCATTGTGTC GCATCCGCAG GTCATTATCA AGGAGGAGCC ACTCAGCCTG 
AGCGACAGTG GTGATGTTGT CAACTCTGTG CCTGTCTATG CCATACAAGC CAATCCCGGT 
GTACCCGCTC CGGCCAGTTC GGGTGTGCTA GTCGGCACAC AAACGGTACC TGCCGATCTG 
GCGCACAAGA TCCGGCACAA ATGTCCGGAT TGTCCAAAGA CCTTCAAGAC GCCCGGCACG 
CTGGCCATGC ACCGCAAGAT ACACACAGGC GAAGCAGACG CCACGCCCAA AGAACGCCCC 
TACACGTGCT CCTACTGCGG CAAGTCCTTC ACTCAATCGA ATACACTAAA ACAGCACACT 
CGCATACATA CAGGTGAGAA ACCATTTAGA TGTGGCTATT GTGGCAGGGC GTTCACTGTT 
AAGGATTACC TGAACAAACA TTTAACGACT CACACGGGAG AGAAGCCGTT CCACTGCGGT 
TACTGCGAGA AGTCCTTCAG CGTGAAGGAC TATCTGACCA AGCACATACG GACGCACACC 
GGCGAGAAGC CGTACACCTG TCCGTATTGC GACAAGCGCT TCACGCAGCG CAGCGCCCTC 
ACTGTGCACA CGACCAAGCT GCATCCGCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523474.1
CDS	320..1852
Translation	MIKSTTNPQEQRLPRPEDQSPAPPPPPPSSATTSTAAPATPTHQVATVIANMDTLKTAFLPNLSMDPNVHVSPHYCPMCHQQFERPQHVADHMQLCHGITLNAQGAIATLDGGHPQAQQHPKLSHPCFNCDEKFGNAVDLDEHHRLAHQTPAFLSRCLMCSIYGIHSATQQPNEYKCTQCGSICTTAMLAAGQQGFMEQQEAAVTPDDQLPAMAPRDMRLTPEEQHHQQQLQAEHHHQQQHQQQQQQQQQQQELLEQQQREMQEQAQQQQVHHHQQDQDLAGDQVALKVPPLTVKLNKNANGGAIVSHPQVIIKEEPLSLSDSGDVVNSVPVYAIQANPGVPAPASSGVLVGTQTVPADLAHKIRHKCPDCPKTFKTPGTLAMHRKIHTGEADATPKERPYTCSYCGKSFTQSNTLKQHTRIHTGEKPFRCGYCGRAFTVKDYLNKHLTTHTGEKPFHCGYCEKSFSVKDYLTKHIRTHTGEKPYTCPYCDKRFTQRSALTVHTTKLHPL

Target ORF information:

RefSeq Version	NM_078750.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chorion factor 2, transcript variant A (Cf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078750.4

ORF Insert Sequence:

ATGATAAAGT CCACCACGAA TCCACAGGAA CAGCGTCTGC CACGCCCCGA GGATCAGTCG 
CCAGCGCCGC CGCCGCCGCC CCCCTCCTCA GCCACCACCT CCACCGCCGC CCCAGCCACG 
CCCACGCACC AAGTGGCCAC CGTGATAGCC AACATGGACA CCCTGAAGAC AGCCTTTCTG 
CCCAATCTCA GCATGGACCC CAATGTGCAC GTGTCGCCGC ACTATTGTCC CATGTGTCAC 
CAGCAGTTTG AGCGACCGCA GCACGTCGCG GATCACATGC AGCTGTGCCA CGGGATCACG 
CTGAACGCCC AGGGAGCCAT TGCCACGCTG GACGGCGGTC ATCCGCAGGC ACAGCAGCAC 
CCCAAGCTCA GCCACCCCTG CTTCAATTGT GATGAAAAGT TTGGCAACGC CGTCGATTTA 
GACGAACACC ATCGGCTGGC CCATCAGACG CCCGCCTTCC TGTCCCGCTG CCTTATGTGC 
AGCATCTATG GCATCCACTC GGCCACCCAG CAGCCCAACG AGTACAAATG CACGCAGTGC 
GGCTCCATTT GCACCACCGC CATGCTGGCT GCCGGACAGC AGGGCTTTAT GGAACAGCAG 
GAGGCGGCGG TGACGCCCGA CGATCAGCTG CCGGCAATGG CGCCCCGTGA TATGAGACTG 
ACGCCCGAGG AGCAGCACCA TCAGCAGCAA CTGCAGGCGG AGCACCACCA TCAGCAACAG 
CACCAACAAC AACAACAGCA GCAGCAGCAG CAGCAGGAAC TGCTGGAGCA GCAGCAGCGT 
GAAATGCAGG AGCAGGCGCA ACAGCAGCAG GTGCACCATC ACCAGCAGGA TCAGGATCTC 
GCCGGCGACC AGGTGGCGCT GAAGGTGCCA CCACTCACCG TCAAGCTAAA CAAGAACGCC 
AACGGTGGCG CCATTGTGTC GCATCCGCAG GTCATTATCA AGGAGGAGCC ACTCAGCCTG 
AGCGACAGTG GTGATGTTGT CAACTCTGTG CCTGTCTATG CCATACAAGC CAATCCCGGT 
GTACCCGCTC CGGCCAGTTC GGGTGTGCTA GTCGGCACAC AAACGGTACC TGCCGATCTG 
GCGCACAAGA TCCGGCACAA ATGTCCGGAT TGTCCAAAGA CCTTCAAGAC GCCCGGCACG 
CTGGCCATGC ACCGCAAGAT ACACACAGGC GAAGCAGACG CCACGCCCAA AGAACGCCCC 
TACACGTGCT CCTACTGCGG CAAGTCCTTC ACTCAATCGA ATACACTAAA ACAGCACACT 
CGCATACATA CAGGTGAGAA ACCATTTAGA TGTGGCTATT GTGGCAGGGC GTTCACTGTT 
AAGGATTACC TGAACAAACA TTTAACGACT CACACGGGAG AGAAGCCGTT CCACTGCGGT 
TACTGCGAGA AGTCCTTCAG CGTGAAGGAC TATCTGACCA AGCACATACG GACGCACACC 
GGCGAGAAGC CGTACACCTG TCCGTATTGC GACAAGCGCT TCACGCAGCG CAGCGCCCTC 
ACTGTGCACA CGACCAAGCT GCATCCGCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cf2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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