ft cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ft
Entrez Gene ID	33627
Full Name	fat
Synonyms	79/18,CG3352,CT11259,Dm Fat,Dmel\CG3352,Fat,Ft,l(2)24Da,l(2)79/18,l(2)fat,l(2)fd,l(2)ft,l(2)gd-1,l(2)gd-l,l(2)gd2,l(2)k07918
General protein information	Preferred Names fat Names CG3352 gene product from transcript CG3352-RA CG3352-PA floppy-discs ft-PA giant discs 2 giant-discs-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 24D8-24D8\|2-12.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_058149.4	NP_477497.1	fat

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FAT4	XP_526682.3
Bos taurus (cattle)	FAT4	XP_005217662.1
Danio rerio (zebrafish)	LOC101882648	XP_005173110.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007924	XP_317558.4
Homo sapiens (human)	FAT4	NP_078858.4
Drosophila melanogaster (fruit fly)	ft	NP_477497.1
Xenopus tropicalis (tropical clawed frog)	fat4	XP_004911174.1
Canis lupus familiaris (dog)	FAT4	XP_005631883.1
	FAT4	XP_002804232.1
Mus musculus (house mouse)	Fat4	NP_899044.3
Gallus gallus (chicken)	FAT4	XP_420617.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

App promotes drosophila growth via the Hippo pathway by palmitoylation of Fat protein.
Title: The palmitoyltransferase Approximated promotes growth via the Hippo pathway by palmitoylation of Fat.

Vamana associates with the Dachsous intracellular domain and with a region of the Fat intracellular domain that is essential for controlling Hippo signaling and levels of Dachs.
Title: Vamana Couples Fat Signaling to the Hippo Pathway.

Dlish (CG10933) tethers Dachs to the subapical cell cortex, an effect partly mediated by the palmitoyltransferase Approximated under the control of Fat. Conversely, Dlish promotes the Fat-mediated degradation of Dachs
Title: The novel SH3 domain protein Dlish/CG10933 mediates fat signaling in Drosophila by binding and regulating Dachs.

Cell Death Changes the Orientation of Cell Division in the Developing Drosophila Wing Imaginal Disc Without Using Fat or Dachsous as Orienting Signals
Title: Local Cell Death Changes the Orientation of Cell Division in the Developing Drosophila Wing Imaginal Disc Without Using Fat or Dachsous as Orienting Signals.

Data proposes that Ft recruits FbxL7 to the proximal side of the cell to help restrict Ds and Dachs to the distal side of the cell.
Title: The ubiquitin ligase FbxL7 regulates the Dachsous-Fat-Dachs system in Drosophila.

The following ft gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ft cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13709	NM_058149.4 Latest version!	Drosophila melanogaster fat (ft), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ft ( NM_058149.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477497.1 Drosophila melanogaster fat (ft), mRNA.

Price & Availability↑

CloneID	OFa13709
Clone ID Related Accession (Same CDS sequence)	NM_058149.4
Accession Version	NM_058149.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 15444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fat
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_058149.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAGGC TACTGCTCCT GTTTTTCCTG CTTCTGGCCG GCAGGGAATC CTTGTGCCAA 
ACAGGCGACA CCAAACTGGA GCTACTGGCA CCGCGTGGCC GAAGTTACGC CACCACCTAT 
GAACAATATG CCGCCTTTCC GCGAAGAAGA AGCTCATCAT CCTCGCCATC GGGTGAGATG 
CAATCCCGTG CGGTGGACAC CAGTGCCGAT TTCGAGGTGC TCGAGGGTCA GCCGCGTGGA 
ACTACGGTTG GTTTTATACC CACAAAACCA AAGTTCTCTT ATCGATTCAA TGAACCTCCT 
CGGGAATTCA CACTCGATCC GGTAACGGGT GAGGTGAAGA CCAATGTGGT GCTCGATCGG 
GAAGGTATGC GGGATCACTA CGATTTGGTG GTGCTCTCCT CACAGCCCAC CTATCCCATC 
GAGGTGCGGA TTAAGGTTCT GGATGTCAAC GACAATTCGC CGGAGTTTCC CGAACCCAGC 
ATCGCGATTT CCTTCTCCGA AAGTGCCACT TCGGGTACTC GATTGCTTCT CGATGCCGCC 
ACAGATGCGG ATGTTGGTGA AAACGGGGTG ACCGATCAGT ATGAGATCGT GGCTGGAAAT 
GTGGACAACA AATTTCGGCT TGTGACTACG GCGAATCCCA GTGGAGACAC CTCGTATCTG 
CATCTAGAAA CCACAGGAAA TCTCGACCGT GAGTCGCGTG GCAGTTACCA ACTAAACATC 
TCTGCCAGAG ACGGAGGATC TCCACCGCGT TTTGGCTACC TCCAAGTGAA CGTTACCATT 
CTGGATGTCA ATGATAATCC ACCGATCTTC GATCACAGCG ATTACAATGT GTCCCTAAAC 
GAAACAGCTC TCCCGGGAAC TCCGGTCGTG ACCGTCATGG CTTCGGACAA CGACTTGGGG 
GACAATAGCA AGATCACCTA CTATCTGGCC GAAACGGAGC ACCAATTCAC GGTGAATCCG 
GAGACGGGAG TCATTTCCAC CACGGAACGC GTAAACTGCC CCCAGCAAAC GAACGTGAAG 
AGCTCTGCTA GCCAGAAAAG CTGCGTTTTC ACTGTATTCG CCCGGGATCA TGGATCTCCA 
CGGCAGGATG GACGCACCTA TGTAACTGTT AATCTGCTGG ACACAAACGA TCACGACCCC 
ATCATCAGTT TCCGCTTCTT TCCGGATGGC GGAAAAGTGG CCACTGTGGA TGAGAATGCG 
GTAAATGGTA CTGTTGTGGC AGCCGTGGCC GTTAAGGATT CCGATTCCGG CCTAAATGGC 
AGAACATCGG TGCGAATTGT TTCCGGCAAT GAGCTGGGAC ACTTTCGCCT AGAAGAAGCT 
GCCGACTTGC ATATAGTAAG GGTAAATGGA GTCCTGGATC GCGAGGAGAT CGGGAAATAC 
AATCTGACCG TGGTGGCAAT GGATCAAGGA ACACCGGCGC GAACGACCAC GGCACATCTT 
ATCATCGACG TAAACGATGT GAATGATCAT GAACCGGTGT TCGAGAAATC AGAATATTCA 
GCGGTGCTGA GTGAACTGGC ACCCACGGGA AGTTTTGTGG CCTCAATTAC TGCCACGGAT 
GAGGATACTG GGGTCAATGC CCAGGTGCAC TATGATATTC TCTCGGGTAA TGAGCTCAAG 
TGGTTCTCCA TGGATCCACT GACAGGTTTG ATTGTGACTA CGGGTCCGTT GGATAGAGAG 
ATTCGCGACA CCGTAGAACT GAGCATCTCG GCCAGAGACG GTGGACCTAA TCCCAAATTT 
GCGTACACTC AGCTGAAGGT GATCATTCTG GACGAGAACG ATGAGGCGCC GCAGTTTAGC 
CAAAGAGAGC AGAATGTGAC TTTAGGTGAG GATGCTCCTC CGCAAACGAT TGTGGCTCTG 
ATGACGGCCA CTGATCATGA TCAGGGCACG AATGGATCGG TGACCTTTGC CCTAGCGCCC 
AGTGTGGAGC GCCTGTATCC GCTACAGTTT GCCCTCGATG CGTTGACGGG CCAATTAACA 
ACGCGAAGGC CTCTGGATCG TGAGAAAATG TCACAGTATG AGATCTCCGT GATTGCCCGC 
GATCAGGGAG CACCCACGCC CCAATCGGCC ACGGCCACCG TTTGGCTGAA TGTGGCCGAT 
GTAAACGATA ACGATCCACA GTTTTATCCC AGGCACTACA TCTACTCGCT CGCCGATGAC 
GATGATGATA TCAAGCTCAA GAAGGAGGTG GAGAAGGAGA GGATCCTGCT CCATGTCACG 
GCCTCAGATA AAGACGATGG CGATAATGCC TTAATTGAGT ACAGACTCGA GTCAGGTGGC 
GAGGGTCTCT TCCAGCTGGA CGCCCGATCG GGTGCCATCA GCCTGCGTGG TGATGCTCCT 
GCATCGATGC ATTGGAAGCC ACACTACAAG CTCCTCGTAT CGGCTCGTGA TGCTGGGCAA 
CGGCGGAGCC AGCAAGATGC CATTGTGGAA ATTGTGCTCA AGTCCAAGTT GGAGATGCTT 
GAATGCGGTC AGGCACAAGC TGGTGGTTAT GAGTTCCAGA TGGTCGAGGA TCACGAGCAG 
CAAAGGAACA GCCAACCAAA TCGCGAGGTG GGCATCGTGC AGGTAAAATC CACGAATGGC 
AAGGCGAACT CTCATATCGA ATATGATATC ATCCAAGGCG ACAGGGCTCA GAATTTCCGA 
ATCGATACCA GAAGTGGTCG CATCACCACC GCCCGACCTT TGGATCGCGA GGAGCAGGCC 
AATTATCGTT TGACTATCTT GGCCAGCTCC AGTTCCAGCT CCAGTGCAGC CGCCAGCAGC 
GTTTCATATG GCCAGTGCAT TGTTAATATA GCCATTATCG ATCTGAATGA TAATGCTCCC 
GTTTTCGCCT TGGATCGCGA GTCAGAGCCC ACAATCTCGC TGCCGGAGAA TGCGGCCGTG 
GGTCAGGAGA TCTACCTATC CAGGGTCAGA GATCGGGATG CGGGCGTGAA TAGTAGGATC 
AGCTATAGCC TCACCAACAA TCCCAATCAG CAGTTCCGCA TTGGACCGGT CACGGGAGTT 
CTCTACCTTC AGAGGCCCAT AAGAGCAGAG CCTGGATCCC TCATCCACGT GGAACTAATG 
GCCACGGATG CGGGTAGTCC TCCGCTCTCC AGTAAACTGA GTCTATCTGT GCTGATAGCC 
GATGTTAACG ATCACACTCC CGTCTTTGAT CACACATCCT ATGAGACTTC CCTCCCAGAA 
ACCACGAAAG TGAACACTCG ATTTTTCGCT TTGGCTGCTA CGGATATTGA TTTGGGGGAC 
AATGGACGGA TCTCGTACGA GATCATCGAG GGCAACACTG AGCGCATGTT CGGTGTCTTT 
CCCGATGGCT ATCTGTTTGT AAGAGCTCCA CTCGATCGTG AGGAGCGGGA TTACTATGCC 
CTAACTGTTT CTTGCCGGGA TGCTGGTCAA CCATCCAGAT CTTCGGTGGT TCCAGTTGTC 
ATCCATGTCA TCGATGAGAA CGACAATGCC CCACAGTTCA CGAACAGCAC ATTTACGTTT 
AGTATTCCGG AAAATGCTCC TGCTGACACG TTTGTGGGAA AGCTAACCGC AGTTGATCGG 
GATATCGGAA GAAATGCCGA GCTCAGTTTT ACCCTGTCCA GTCAGACGCA GGACTTTACG 
ATTGACACCA GGAATGGTTT CATAAAAACT CTGCGACCCT TCGATCGCGA GGCTCTGGTG 
AAAGTCAGCA GAAATGCGGA GGCAAGCGGA GAGGATGGTT CTCTCAGGGG TTCAATGGCT 
GGCAACTATA TGCTACTGGA GGCCACAGTT TCGGACAATG GCATTCCTCG CTTACAGGAC 
AAAGTCAAGG TGAAGGTCAT CGTCACCGAT GTCAACGATA ATGCACCGGA ATTCCTGAGA 
GCACCCTATC ATGTTACCAT TTCTGAGGGC GCCTCTGAGG GCACGCATAT AACGCATGTC 
TTTACCCAAG ATGCGGATGA GGGTCTGAAT GGAGATGTCT ACTACTCACT GGCGAAGGGA 
AATGAGGCTG GTCAGTTCAA TTTGGATAGC GCCACAGGAC AGCTTTCGTT GGGGCGCAGG 
TTGGACCGGG AGTCCCAGGA GATTCATCAC CTGATCGTCG TGGCCAAGGA TGCTGCTCTC 
AAGCATCCGC TGAGCTCTAA TGCTAGCATT ACAATTGTGG TTCTGGACGA GAACGACAAT 
GCTCCGGAGT TCACGCAGTC GAGCAGTGAG GTTTCCGTTT TGGAGACAAG TCCAACGGGC 
ACTGAATTGA TGAGATTCCG AGCTAGTGAT GCGGATCAAG GGGTAAATAG CCAGGTGGTC 
TTCAGTATAT CGGCTGGAAA TCGCCGTGAT ACCTTCCACA TCGACAGCAT TACGGGTAGC 
CTCTATCTGC ACAAGCCTCT GGACTACGAG GACATAACCA GCTATACTCT CAACATCACT 
GCCTCAGATT GTGGCACTCC GTCGTTGTCT ACCACCGTTT TGTATAACGT TCTCGTCGTG 
GATGACAACG ACAATCCGCC CATCTTTCCC AGCACTGCGA TTGTTCGGCA GATCAAGGAG 
GGAATTCCCC TGAAAACACC CATTGTGACC GTAACCGCCG ACGATCCGGA TTCGGGACTT 
AACGGCAAGG TTAGCTATGC CATTAGCAAG CAGGAACCCC AACTTCCACA GGGACGACAC 
TTTGGCATAA ACACGGAAAC GGGTGTGATT CACACGCTGA GGGAGATTGA TCGCGAATCC 
ATTGATACCT TTCGGCTAAC CGTGGTGGCC ACGGATCGTG CTCAGCCTTC GGAGCGCCAG 
CTCTCGACGG AAAAACTGGT CACCGTGATT GTGGAGGACA TAAACGATAA CGCACCAGTT 
TTTGTGTCCA TGAATGCGGC TATCCTGCCT CCAAAGTTCT CGACTTCCAA GGGCAGCAGC 
ACAGCGGTGA TGCAGGTTCA TGCCAAGGAT GCGGACTCGT CCAGCAACGG ATTGGTGACC 
TACGAGATTG TCAGTGGCCC CCAGGAGCTG TTCAAGCTGC AAAGGAACAC GGGTATCATA 
ACCTTTACCC CAGGTCCTCA GTTCAAGCAG GAGGTTCGCT ACCAGCTGAC CCTTAAGTCC 
ACCGACGAAG CCGTTCAAAG CGAACGTCGC AGCAGCGAGG TCTACATTAC AATCATAACG 
CCAGGCTCCG GCGGAAGTGA GTCCTCCGTT CCACAGTTCG AGCAACGGAG TAAGCTTTCC 
GGTTCCGTCT ACGAGAATGA ACCAATTGGA ACTAGTATTC TGACGGTGAC TGCTCATCTG 
GCGTCCGCCG AGATAGAATA CTTTGTGACG AATGTTACGG CAACCGGATC GCGTGGTCAG 
GTAGATCGCC TCTTCGACAT CGATGCCAAG CTGGGAATTC TGTCCACCGC CGCTGAACTG 
GACCGTGAAG CCGGACCAGA GGAGTACGAG GTGGAGGTAT ACGCCATCGC ACTTGGCGGA 
CAGCCACGTA CATCCCGAAC GAAGGTCAGA GTAACCGTTC TCGACAAGAA CGATAGTCCA 
CCGCAGTTCC TCGACACACC GTTCGTCTAC AATGTCAGCG AGGATCTACA GATTGGACAC 
ACCATCTCTA CGCTGCGAGC CCACGATCCG GATACGCTTG GTTCGGTGAC GTTCCTTCTG 
ATGGATGGGC ACGATGGCAA GTTCCTGCTG GAACCGTCCA CTGGAAAACT GATCCTCAAC 
GATACCCTCG ATCGGGAGAC AAAGAGCAAG TATGAGCTAA GGATTCGCGT ATCCGACGGA 
GTGCAGTACA CAGAGGCATA TGCCACTATA CAGGTGAGCG ATACCAATGA TAATCCACCC 
TTATTCGAGG ATACCGTGTA CTCCTTTGAC ATACCGGAAA ATGCCCAACG GGGCTACCAA 
GTGGGTCAGA TTGTGGCCAG GGATGCCGAT CTGGGCCAAA ATGCCCAATT GTCCTACGGC 
GTGGTCTCGG ATTGGGCCAA CGATGTCTTC AGCCTGAATC CACAAACAGG AATGCTGACC 
CTAACTGCGC GACTGGATTA CGAAGAGGTT CAGCACTATA TCCTGATTGT TCAGGCGCAG 
GATAATGGCC AACCCAGCTT GTCCACCACC ATAACGGTCT ACTGCAATGT CCTCGATCTG 
AACGATAATG CACCTATTTT CGATCCCATG AGCTATTCGA GCGAGGTGTT CGAGAATGTG 
CCCATCGCCA CAGAGGTAGT CACTGTGTCA GCAAAGGATA TAGATTCGGG CAACAATGGG 
CTCATTGAGT ACAGCATCAC CGCAGGAGAT GTCGACTCAG AGTTTGGAAT CGATAGCAAT 
GGCACTATAC GCACTCGCCG CAACTTGGAC AGGGAACACC GATCGACGTA CACACTTACG 
GTTACCGCCC GAGATTGTGC CGATGAATTT GCATCCTTCA GCGAACTGGA GGAGACGCAG 
CTGAAGCTCA AGTATCGATC GCCTAGAAAG TACCAACAAA CACGTCAAGA GTTTTTGGCC 
CATCAAAAGC AGCAGCGGCT CTCCTCGACT GTAAAGGTGA CGATACTCAT CAAAGATGTC 
AACGACGAAG TGCCCGTCTT CATTTCCGCA AACGAAACGG CGATCATGGA GAATGTGGCC 
ATCAATACGG TCGTTATCGC TGTCAAGGCT GTCGACAATG ACGAGGGCCG CAACGGATAC 
ATCGATTATC TGATGAAGGA GGCCAGAGAT GAGGACATGG GTCAATCGGA TCCGCTGCCC 
TTCTCCCTGA ATCCCACCGA TGGACAGTTG CGCGTAGTCG ATGCCTTGGA CCGTGAATTG 
CGCTCAAGTT ATTTGCTAAA CATCACAGCC CGAGATCGTG GAGAGCCACC CCAGTCCACG 
GAGTCGCAGT TGCTCATTCG AATTCTGGAC GAGAACGACA ATAGTCCCGT CTTCGATCCG 
AAGCAATACT CTGCCTCGGT GGCGGAAAAC GCCAGCATTG GTGCCATGGT CCTTCAGGTA 
TCCGCCACGG ATGTGGATGA AGGTGCAAAT GGACGCATCC GCTATTCCAT AGTGCTGGGC 
GATCAGAACC ATGACTTCAG CATCAGCGAG GATACGGGTG TGGTGCGGGT GGCTAAGAAC 
CTAAACTACG AACGACTTTC CCGATACTCG CTCACCGTAC GCGCCGAGGA TTGTGCCTTG 
GAGAATCCTG CTGGCGACAC TGCCGAGCTG ACCATCAATA TTCTGGACAT AAACGATAAT 
AGACCGACAT TTTTGGATTC CCCATATTTG GCACGTGTCA TGGAGAACAC TGTACCGCCA 
AATGGTGGAT ATGTGCTCAC TGTAAACGCC TATGATGCGG ACACTCCACC GCTTAATTCG 
CAGGTGCGCT ACTTTCTCAA GGAAGGCGAT AGTGATCTCT TCCGCATCAA TGCCTCTTCG 
GGTGACATCG CGCTGTTGAA ACCCCTGGAT CGAGAGCAGC AGAGCGAGTA CACCCTGACA 
CTGGTGGCCA TGGACACGGG TTCGCCTCCA CTTACGGGCA CGGGAATTGT GCGAGTGGAG 
GTGCAGGACA TTAATGACAA CGATCCGGTA TTCGAACTGC AATCCTATCA TGCCACTGTG 
CGCGAGAATT TACCGTCGGG AACTCATGTC CTGACCCCCA GAGCAACGGA TAAGGACGAA 
GGACTAAACG CCAAGCTGCG CTTTAATTTG TTGGGAGAGC ACATGCATCG GTTTCACATA 
GATTCAGAAA CGGGCGAGAT TAGCACAGCT ACAACTCTTG ATCGTGAAGA GACTTCCGTG 
TACCACTTGA CCTTAATGGC CCAGGATAGC TCGATTACCG AGCCACGAGC ATCGTCTGTA 
AATCTCACAA TCAGTGTGTC CGATGTCAAT GACAATATCC CGAAATTCGA TTCGACCACC 
TACAACGTAG CAGTTCCGGA GAGGATCAGC AAGGGTGAGT TTGTGTTTGG AGCTCGAGCC 
TTAGACTTGG ATGATGGCGA GAATGCTGTG GTACATTACA CGATTAGCGG GCGAGATCAA 
CATTACTTTG ATATCAACAC CAAAACGGGT GTGGTGAGCA CCAAGCTGGA GCTTAAGACC 
AAGACCAAGT CTCACGATGA TTTAACCTAT ACGATTGTGA TATCTGCAAT GGATCAGGGC 
GAGCAATCTC TTAGTTCCAA GGCTGAGCTT ACAGTTATCC TCCGACCACC GGAGCTCTTT 
CCCACCTTTG CCTACATGGC CAACAGTCAC TTTGCCATGT CAGAGGATGT TAGGCCGGGC 
AAGATGATAA CCAAAGTCAG TGCCACATCG CCCAAAAAGG GTTTGGTGGG TAAAATTCGC 
TATGCCATAG CCGGCGGAAT AATGGGGGAT TCTCTGAGAG TGGATCCCAA TAGCGGATTA 
CTGAGCGTTG GTCAGGATGG CCTGGACTAT GAGCTGACAC ATCTGTATGA GATTTGGATT 
GAGGCCGCCG ATGGAGACAC GCCAAGTCTG CGTAGTGTAA CTCTTATAAC GCTCAACGTA 
ACGGATGCCA ATGACAATGC TCCCGTTATG GAACAGCTCA TTTACAATGC CGAGGTCCTG 
GAGGAGGAGT CCCCACCGCA GTTGATAGCC GTTGTGAAAG CCAGCGATCG TGACTCCGGC 
GACAATGGAA ATGTCATCTA TCGCCTACAG AATGACTTCG ACGGCACATT TGAGATTACG 
GAATCGGGTG AGATTTACAC CAGGATGCGA CTGGATCGCG AGGAGATCGG CGATTATGCG 
TTCGTAGTGG AGGCCGTTGA CCAGGGTGTT CCTCATATGA CGGGAACAGC TAGTGTCCTA 
CTGCATCTAC TGGACAAGAA CGACAACCCG CCCAAGTTCA CACGCCTCTT TTCCCTGAAT 
GTAACCGAGA ACGCCGAGAT CGGTAGCTTT GTGATCCGAG TCACCTCTTC TGATTTGGAT 
CTGGGTGCAA ATGCCAATGC TTCTTACTCC TTCAGCGAGA ATCCCGGCGA GAAGTTTCGC 
ATTGAGCCGC AGAGTGGAAA CATCACAGTG GCCGGTCATC TTGATCGCGA GCAACAGGAT 
GAGTACATCC TGAAAGTGGT GGCCTCTGAT GGAGCCTGGC GTGCCGAGAC ACCCATTACC 
ATCACAATCC AGGACCAGAA TGACAATGCC CCCGAATTCG AGCATAGCTT CTACAGCTTC 
AGTTTCCCGG AGCTGCAGCA ATCGATTGCT TTGGTGGGCC AGATCATAGC CACGGATCGC 
GATAAACAGG GTCCCAACTC AGTGATCTCC TACTCGCTGC AGCAGCCGTC GCCCATGTTC 
AGCATAGATC CAGCCACGGG TGAGGTCTTC AGTAAGAAGG CGGTTCGCTT CAAGCACTCT 
CAGTACGTTC GCTCACCGGA GAATATGTAC GCCCTCACCG TTCTGGCCAC GGATAATGGT 
AAGCCTCCTC TGTACTCCGA GTGCCTGGTG AACATCAATA TCGTCGACGC CCACAACAAT 
CCACCAAAGT TCGAACAGGC GGAATATCTG GCGCCGCTGC CACAGGATGC AGTTCGTGGT 
CAGCGAATAG TTCGAGTCCA CGCAAATGAC AAGCAGGATC TGGGCACCAA CGAAATGGAC 
TACTCCCTGA TGACATTCAA TTTGAGTTCG ATCTTCAGTG TCGGCAGACA CGATGGTTGG 
ATTACTTTGG TCAAACCCAT TCAAGTGCCG CCAAACACTC GATATGAACT GGTTGTTAGG 
GCCACCGATC GTGGTGTTCC TCCCCAGTCC GATGAGACAC GAGTCGTGAT CGTGGTAACC 
GGGGAGAATA TGGACACACC CAGATTTTCC GTAAACAGCT ATCAAGTCAT TGTGCCCGAG 
AATGAGCCTG TTGGGTCCAC AATTCTAACT GTGGGCGCCA CTGACGATGA TACGGGTCCA 
AATGGAATGC TGCGCTACTC CATTTCCGGT GGCAATGAGC GTCAAGACTT CAGTGTGGAT 
GAACGTACGG GTGGCATAGT GATACAACAG CAATTGGATT ATGACCTTAT ACAGGAATAC 
CATTTGAATA TCACAGTTCA GGATCTGGGC TACCATCCAC TATCCTCAGT TGCAATGCTT 
ACGATCATTC TGACTGATGT TAATGACAAT CCACCTGTGT TCAATCACAA GGAATACCAC 
TGTTATATAC CTGAAAACAA ACCGGTTGGC ACCTTTGTAT TCCAGGCTCA TGCCGCGGAT 
AAGGATTCAC CGAAAAATGC TATTATCCAT TATGCATTCC TGCCCAGCGG ACCAGACAGG 
CACTTCTTCA TAATGAACCA GAGTAATGGC ACCATTTCTT CGGCAGTTTC CTTTGACTAT 
GAAGAGCGCC GCATCTACAC ACTACAGATA AAGGCGAAAA ATCCCGATTC GAGCATGGAG 
AGCTACGCCA ATCTCTATGT GCATGTCCTG GGCGTGAATG AGTTTTATCC ACAATTCCTG 
CAGCCCGTCT TTCACTTTGA CGTATCGGAA ACATCGGCGG TGGGCACTAG AGTGGGTGCC 
GTTCAGGCCA CCGATAAGGA CAGCGGCGAA GATGGACGTG TCTACTACCT TCTGGTGGGT 
TCCAGTAACG ACAAGGGTTT CCGCATCGAT ACCAATACGG GCTTGATTTA TGTAGCCCGA 
CACTTGGACA GGGAGACACA GAACCGTGTG GTACTCACAG TGATGGCCAA AAACTATGGC 
AGTATTCGTG GTAACGACAC TGACGAGGCA CAAGTGATTA TATCGATTCA GGATGGCAAC 
GATCCGCCGG AGTTCATAAA GCACTACTAT ACCTCCACGA TTTCCGAGGC AGCTCCGGTT 
GGCACGAAGG TCACAACGGT CAAGGCCATT GACAAGGATG TGCGCACACA AAATAATCAG 
TTTAGCTATT CGATCATTAA TGGCAACCTG AAACAAAGCT TTAAGATAGA TGTGCAAACG 
GGTGAGATTA GCACGGCAAG TCGTTTGGAT CGTGAGGAGA CATCCACCTA TAACTTGGTG 
ATTGGAGCCA TTGACACGGG ACTGCCTCCA CAGACGGGAA GTGCAACGGT GCATATCGAA 
CTAGAAGATG TGAACGATAA TGGACCCACA TTCACACCTG AGGGTCTGAA TGGTTATATC 
TCCGAGAATG AACCGGCTGG CACTTCCATT ATGACACTAA TAGCCAGTGA TCCCGATCTA 
CCGCGCAATG GAGGACCCTT CACCTATCAG TTGATTGGTG GTAAGCACAA GTCCTGGCTA 
AGTGTGGACA GAAACTCTGG TGTGGTTAGG TCCACAACCA GTTTCGATCG CGAAATGACA 
CCCATTCTAG AGGCCATAAT TGAAGTCGAG GACAGTGGAA AGCCCAAACA AAAGTCCCAG 
CACCTGCTCA CGATCACTGT ATTGGATCAG AACGATAATC CATCGACCAC GAGGAGCTTG 
CACATAGCAG TCAGTCTGTT CAACGGCGAT CTGCCTTCGA ATGTGAAGCT GGCCGATGTG 
CGTCCCAATG ATATTGACAT CGTGGGCGAT TATCGTTGTC GCCTCCAGAA GAATCCCGCC 
CAGAGTCAAC TGCAGTTGGC TATTCCTCGG GCCTGTGATC TGATCACCAC CTCACATACG 
ACGCCAATAG CTTCGGTTTT CAGCTATACG GGAAATGACG GAAAACATGG GGATGTTAGC 
TCCAAAGTTA GTGTCGCCTT CCAGAGTTTT AACAACGAAA CATTGGCCAA CTCAGTGTCC 
ATCATGGTAC GAAATATGAC AGCTTACCAC TTCTTGGCTA ATCACTATCG ACCAATTCTG 
GAGATGATTA AGTCTAGAAT GAGTAACGAG GATGAGGTCA TTCTATATAG TCTTCTAGAG 
GGTGGCAGTG GCAATTCCAC CAATCTCCAA CTGCTAATGG CAGTCAGATT GGCGAAGACC 
AGTTACCAAC AACCCAAATA TTTGATTGAA CGCTTGCGAG AGAAGCGTTC AGCGTTTTCG 
GAACTCCTGC AAAAGGAGGT GATTGTGGGC TATGAACCCT GCAGTGAACC GGATGTTTGT 
GAAAATGGCG GAGTCTGCAG TGCCACCATG CGACTGCTGG ATGCCCATAG CTTTGTTATC 
CAAGACAGTC CGGCCTTGGT GCTGAGTGGT CCTCGGGTTG TGCACGACTA CAGCTGCCAG 
TGCACCAGTG GATTCTCGGG CGAGCAGTGC AGTCGTCGGC AGGATCCTTG CCTGCCCAAT 
CCTTGCCATT CGCAGGTCCA ATGCCGTCGC CTGGGTAGCG ATTTCCAGTG CATGTGTCCT 
GCCAATCGGG ATGGCAAGCA CTGCGAGAAG GAACGCAGTG ACGTGTGCTA TAGCAAGCCG 
TGTCGCAATG GAGGAAGTTG CCAACGCAGT CCGGACGGAT CCTCCTACTT TTGCCTATGT 
CGTCCCGGAT TCCGTGGCAA TCAGTGCGAG AGCGTGTCGG ACTCATGCCG ACCCAATCCC 
TGTCTGCACG GTGGCTTGTG TGTTAGTCTC AAGCCAGGAT ACAAATGCAA CTGCACGCCG 
GGACGATATG GACGACATTG CGAGCGTTTT AGCTATGGAT TCCAACCGCT GTCCTACATG 
ACTTTTCCCG CTCTGGATGT GACCACCAAT GATATATCCA TTGTGTTCGC CACGACGAAA 
CCCAACTCCT TGTTGCTGTA CAACTACGGA ATGCAGAGTG GCGGCAGATC CGATTTCCTG 
GCCATCGAAC TGGTGCACGG CAGGGCGTAT TTCTCATCTG GAGGAGCTCG TACTGCTATC 
AGTACTGTGA TTGCTGGACG AAATCTGGCT GACGGTGGAT GGCACAAGGT TACGGCCACC 
CGGAATGGAC GTGTGATGTC ACTCAGCGTG GCCAAGTGCG CAGATTCCGG TGACGTTTGT 
ACGGAATGCC TGCCCGGAGA TTCTAGCTGC TATGCGGACG AAGTGGGACC AGTGGGAACT 
CTCAACTTCA ACAAACAGCC CCTGATGATC GGTGGCTTGT CCTCGGCTGA TCCTATCTTG 
GAGCGACCTG GTCAAGTGCA CAGTGATGAT CTGGTGGGCT GTCTGCACAG TGTTCATATC 
GGAGGACGTG CCCTGAACCT CAGCTTGCCG CTTCAGCAGA AGGGCATTTT AGCCGGATGC 
AATCGGCAGG CGTGTCAACC AGCTTTGGCC GCCGAACGCT GTGGAGGATT CGCTGGACAA 
TGCATCGATC GGTGGTCCAG TTCGCTATGT CAGTGCGGCG GACACTTACA GTCGCCCGAT 
TGCTCGGACT CCTTGGAACC CATCACCCTG GGCGAAGGTG CCTTTGTGGA GTTTAGAATT 
TCGGAAATAT ATCGTCGCAT GCAGCTGCTG GACAATCTGT ATAACAGCAA AAGTGCCTGG 
CTGGATAACC AACAGATGAG AGAACGTCGA GCCGTAAGCA ATTTCAGCAC AGCTTCCCAG 
ATCTACGAGG CTCCAAAGAT GCTTAGCATG CTGTTCCGCA CCTATAAGGA TCAGGGTCAG 
ATCCTTTATG CGGCCACCAA TCAAATGTTT ACCTCACTGT CCCTGCGCGA GGGTCGTCTG 
GTTTACTACT CCAAACAGCA TCTTACCATC AATATGACGG TGCAGGAAAC TTCTACGCTC 
AACGATGGCA AGTGGCACAA TGTGAGCTTG TTTAGCGAGA GTCGAAGTCT GCGACTCATT 
GTCGATGGAA GGCAAGTGGG TGATGAGCTG GATATTGCCG GGGTGCACGA CTTCCTCGAT 
CCCTATTTGA CCATTCTGAA TGTGGGTGGC GAAGCATTTG TTGGTTGCCT GGCCAATGTG 
ACGGTGAACA ACGAACTGCA GCCGCTAAAC GGATCAGGCA GCATCTTTCC GGAGGTTCGT 
TATCACGGCA AGATTGAGTC CGGCTGCAGA GGAGACATTG GCCAAGATGC CGCCCAGGTG 
GCCGATCCTC TCAGCATTGG CTTCACCCTG GTCATTGTCT TCTTCGTCAT TCTGGTGGTG 
GCTATACTGG GCTCCTATGT TATCTATCGA TTCCGCGGAA AGCAGGAGAA GATTGGTAGT 
TTGAGTTGCG GTGTGCCCGG CTTCAAGATT AAGCACCCTG GTGGTCCAGT GACGCAGAGT 
CAGGTGGATC ACGTCTTGGT TCGCAATCTG CATCCCAGTG AAGCCCCGTC ACCGCCAGTT 
GGAGCTGGAG ATCATATGCG ACCGCCAGTG GGTAGCCATC ACCTGGTGGG TCCCGAACTA 
TTGACCAAGA AGTTTAAGGA ACCCACTGCG GAGATGCCAC AGCCGCAGCA GCAGCAGCAA 
CGTCCCCAGC GACCCGATAT CATAGAGCGC GAGAGTCCTC TGATAAGGGA GGATCATCAC 
CTGCCCATTC CGCCGCTTCA TCCTCTCCCG CTGGAGCACG CCAGTTCCGT GGACATGGGT 
TCCGAGTACC CGGAACACTA CGACCTCGAG AACGCCAGCT CCATTGCTCC GTCCGACATT 
GATATAGTCT ATCATTACAA GGGCTATCGT GAGGCTGCTG GCCTACGCAA ATACAAGGCC 
AGTGTTCCAC CTGTTTCCGC CTACACGCAT CACAAGCATC AGAACTCTGG CTCTCAGCAG 
CAGCAGCAGC AGCACCGCCA CACAGCTCCC TTTGTGACCC GGAACCAAGG TGGCCAACCG 
CCACCGCCGC CCACCAGTGC ATCCCGCACC CATCAGAGCA CTCCACTGGC CCGACTCTCG 
CCCAGCTCGG AGTTGAGTTC CCAGCAGCCG CGCATTCTCA CTTTGCACGA CATTTCCGGA 
AAGCCGCTGC AAAGTGCCCT GCTGGCCACG ACCTCCAGTT CCGGTGGCGT TGGCAAGGAC 
GTTCACAGCA ACAGCGAACG CAGTCTGAAC AGCCCGGTTA TGTCGCAGCT GTCCGGCCAA 
AGTTCCAGTG CCAGCAGGCA AAAGCCCGGA GTGCCACAGC AGCAGGCGCA GCAAACTTCC 
ATGGGCTTGA CCGCCGAGGA GATTGAGAGA TTGAATGGCA GACCACGAAC TTGTAGCCTA 
ATCTCCACCC TGGATGCCGT CTCCTCCAGC AGTGAGGCGC CTCGAGTGTC GAGCAGCGCT 
CTGCATATGT CGCTGGGTGG AGATGTGGAT GCCCATAGTT CGACTTCCAC GGACGAAAGC 
GGCAACGACA GCTTCACGTG CTCGGAGATC GAGTACGACA ATAACAGTCT CAGTGGCGAC 
GGCAAGTATT CCACCAGCAA GAGTCTCCTC GATGGACGCA GTCCCGTGTC CAGAGCACTA 
AGCGGTGGTG AGACGAGCAG AAATCCACCG ACAACTGTGG TCAAGACACC GCCCATTCCG 
CCGCACGCCT ACGATGGATT CGAGTCATCA TTTCGCGGAT CGCTCAGCAC GCTCGTGGCC 
AGCGACGATG ATATAGCCAA TCACCTGAGC GGTATCTACC GCAAGGCCAA TGGAGCCGCA 
TCCCCATCGG CCACCACCCT CGGCTGGGAG TATCTGCTCA ATTGGGGACC TAGCTACGAA 
AATCTGATGG GCGTCTTCAA GGACATTGCC GAGCTGCCGG ACACAAATGG ACCGTCGCAG 
CAACAGCAGC AGCAAACCCA AGTGGTGTCC ACGCTACGAA TGCCATCATC GAATGGACCG 
GCGGCTCCAG AGGAGTACGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477497.1
CDS	681..16124
Translation	MERLLLLFFLLLAGRESLCQTGDTKLELLAPRGRSYATTYEQYAAFPRRRSSSSSPSGEMQSRAVDTSADFEVLEGQPRGTTVGFIPTKPKFSYRFNEPPREFTLDPVTGEVKTNVVLDREGMRDHYDLVVLSSQPTYPIEVRIKVLDVNDNSPEFPEPSIAISFSESATSGTRLLLDAATDADVGENGVTDQYEIVAGNVDNKFRLVTTANPSGDTSYLHLETTGNLDRESRGSYQLNISARDGGSPPRFGYLQVNVTILDVNDNPPIFDHSDYNVSLNETALPGTPVVTVMASDNDLGDNSKITYYLAETEHQFTVNPETGVISTTERVNCPQQTNVKSSASQKSCVFTVFARDHGSPRQDGRTYVTVNLLDTNDHDPIISFRFFPDGGKVATVDENAVNGTVVAAVAVKDSDSGLNGRTSVRIVSGNELGHFRLEEAADLHIVRVNGVLDREEIGKYNLTVVAMDQGTPARTTTAHLIIDVNDVNDHEPVFEKSEYSAVLSELAPTGSFVASITATDEDTGVNAQVHYDILSGNELKWFSMDPLTGLIVTTGPLDREIRDTVELSISARDGGPNPKFAYTQLKVIILDENDEAPQFSQREQNVTLGEDAPPQTIVALMTATDHDQGTNGSVTFALAPSVERLYPLQFALDALTGQLTTRRPLDREKMSQYEISVIARDQGAPTPQSATATVWLNVADVNDNDPQFYPRHYIYSLADDDDDIKLKKEVEKERILLHVTASDKDDGDNALIEYRLESGGEGLFQLDARSGAISLRGDAPASMHWKPHYKLLVSARDAGQRRSQQDAIVEIVLKSKLEMLECGQAQAGGYEFQMVEDHEQQRNSQPNREVGIVQVKSTNGKANSHIEYDIIQGDRAQNFRIDTRSGRITTARPLDREEQANYRLTILASSSSSSSAAASSVSYGQCIVNIAIIDLNDNAPVFALDRESEPTISLPENAAVGQEIYLSRVRDRDAGVNSRISYSLTNNPNQQFRIGPVTGVLYLQRPIRAEPGSLIHVELMATDAGSPPLSSKLSLSVLIADVNDHTPVFDHTSYETSLPETTKVNTRFFALAATDIDLGDNGRISYEIIEGNTERMFGVFPDGYLFVRAPLDREERDYYALTVSCRDAGQPSRSSVVPVVIHVIDENDNAPQFTNSTFTFSIPENAPADTFVGKLTAVDRDIGRNAELSFTLSSQTQDFTIDTRNGFIKTLRPFDREALVKVSRNAEASGEDGSLRGSMAGNYMLLEATVSDNGIPRLQDKVKVKVIVTDVNDNAPEFLRAPYHVTISEGASEGTHITHVFTQDADEGLNGDVYYSLAKGNEAGQFNLDSATGQLSLGRRLDRESQEIHHLIVVAKDAALKHPLSSNASITIVVLDENDNAPEFTQSSSEVSVLETSPTGTELMRFRASDADQGVNSQVVFSISAGNRRDTFHIDSITGSLYLHKPLDYEDITSYTLNITASDCGTPSLSTTVLYNVLVVDDNDNPPIFPSTAIVRQIKEGIPLKTPIVTVTADDPDSGLNGKVSYAISKQEPQLPQGRHFGINTETGVIHTLREIDRESIDTFRLTVVATDRAQPSERQLSTEKLVTVIVEDINDNAPVFVSMNAAILPPKFSTSKGSSTAVMQVHAKDADSSSNGLVTYEIVSGPQELFKLQRNTGIITFTPGPQFKQEVRYQLTLKSTDEAVQSERRSSEVYITIITPGSGGSESSVPQFEQRSKLSGSVYENEPIGTSILTVTAHLASAEIEYFVTNVTATGSRGQVDRLFDIDAKLGILSTAAELDREAGPEEYEVEVYAIALGGQPRTSRTKVRVTVLDKNDSPPQFLDTPFVYNVSEDLQIGHTISTLRAHDPDTLGSVTFLLMDGHDGKFLLEPSTGKLILNDTLDRETKSKYELRIRVSDGVQYTEAYATIQVSDTNDNPPLFEDTVYSFDIPENAQRGYQVGQIVARDADLGQNAQLSYGVVSDWANDVFSLNPQTGMLTLTARLDYEEVQHYILIVQAQDNGQPSLSTTITVYCNVLDLNDNAPIFDPMSYSSEVFENVPIATEVVTVSAKDIDSGNNGLIEYSITAGDVDSEFGIDSNGTIRTRRNLDREHRSTYTLTVTARDCADEFASFSELEETQLKLKYRSPRKYQQTRQEFLAHQKQQRLSSTVKVTILIKDVNDEVPVFISANETAIMENVAINTVVIAVKAVDNDEGRNGYIDYLMKEARDEDMGQSDPLPFSLNPTDGQLRVVDALDRELRSSYLLNITARDRGEPPQSTESQLLIRILDENDNSPVFDPKQYSASVAENASIGAMVLQVSATDVDEGANGRIRYSIVLGDQNHDFSISEDTGVVRVAKNLNYERLSRYSLTVRAEDCALENPAGDTAELTINILDINDNRPTFLDSPYLARVMENTVPPNGGYVLTVNAYDADTPPLNSQVRYFLKEGDSDLFRINASSGDIALLKPLDREQQSEYTLTLVAMDTGSPPLTGTGIVRVEVQDINDNDPVFELQSYHATVRENLPSGTHVLTPRATDKDEGLNAKLRFNLLGEHMHRFHIDSETGEISTATTLDREETSVYHLTLMAQDSSITEPRASSVNLTISVSDVNDNIPKFDSTTYNVAVPERISKGEFVFGARALDLDDGENAVVHYTISGRDQHYFDINTKTGVVSTKLELKTKTKSHDDLTYTIVISAMDQGEQSLSSKAELTVILRPPELFPTFAYMANSHFAMSEDVRPGKMITKVSATSPKKGLVGKIRYAIAGGIMGDSLRVDPNSGLLSVGQDGLDYELTHLYEIWIEAADGDTPSLRSVTLITLNVTDANDNAPVMEQLIYNAEVLEEESPPQLIAVVKASDRDSGDNGNVIYRLQNDFDGTFEITESGEIYTRMRLDREEIGDYAFVVEAVDQGVPHMTGTASVLLHLLDKNDNPPKFTRLFSLNVTENAEIGSFVIRVTSSDLDLGANANASYSFSENPGEKFRIEPQSGNITVAGHLDREQQDEYILKVVASDGAWRAETPITITIQDQNDNAPEFEHSFYSFSFPELQQSIALVGQIIATDRDKQGPNSVISYSLQQPSPMFSIDPATGEVFSKKAVRFKHSQYVRSPENMYALTVLATDNGKPPLYSECLVNINIVDAHNNPPKFEQAEYLAPLPQDAVRGQRIVRVHANDKQDLGTNEMDYSLMTFNLSSIFSVGRHDGWITLVKPIQVPPNTRYELVVRATDRGVPPQSDETRVVIVVTGENMDTPRFSVNSYQVIVPENEPVGSTILTVGATDDDTGPNGMLRYSISGGNERQDFSVDERTGGIVIQQQLDYDLIQEYHLNITVQDLGYHPLSSVAMLTIILTDVNDNPPVFNHKEYHCYIPENKPVGTFVFQAHAADKDSPKNAIIHYAFLPSGPDRHFFIMNQSNGTISSAVSFDYEERRIYTLQIKAKNPDSSMESYANLYVHVLGVNEFYPQFLQPVFHFDVSETSAVGTRVGAVQATDKDSGEDGRVYYLLVGSSNDKGFRIDTNTGLIYVARHLDRETQNRVVLTVMAKNYGSIRGNDTDEAQVIISIQDGNDPPEFIKHYYTSTISEAAPVGTKVTTVKAIDKDVRTQNNQFSYSIINGNLKQSFKIDVQTGEISTASRLDREETSTYNLVIGAIDTGLPPQTGSATVHIELEDVNDNGPTFTPEGLNGYISENEPAGTSIMTLIASDPDLPRNGGPFTYQLIGGKHKSWLSVDRNSGVVRSTTSFDREMTPILEAIIEVEDSGKPKQKSQHLLTITVLDQNDNPSTTRSLHIAVSLFNGDLPSNVKLADVRPNDIDIVGDYRCRLQKNPAQSQLQLAIPRACDLITTSHTTPIASVFSYTGNDGKHGDVSSKVSVAFQSFNNETLANSVSIMVRNMTAYHFLANHYRPILEMIKSRMSNEDEVILYSLLEGGSGNSTNLQLLMAVRLAKTSYQQPKYLIERLREKRSAFSELLQKEVIVGYEPCSEPDVCENGGVCSATMRLLDAHSFVIQDSPALVLSGPRVVHDYSCQCTSGFSGEQCSRRQDPCLPNPCHSQVQCRRLGSDFQCMCPANRDGKHCEKERSDVCYSKPCRNGGSCQRSPDGSSYFCLCRPGFRGNQCESVSDSCRPNPCLHGGLCVSLKPGYKCNCTPGRYGRHCERFSYGFQPLSYMTFPALDVTTNDISIVFATTKPNSLLLYNYGMQSGGRSDFLAIELVHGRAYFSSGGARTAISTVIAGRNLADGGWHKVTATRNGRVMSLSVAKCADSGDVCTECLPGDSSCYADEVGPVGTLNFNKQPLMIGGLSSADPILERPGQVHSDDLVGCLHSVHIGGRALNLSLPLQQKGILAGCNRQACQPALAAERCGGFAGQCIDRWSSSLCQCGGHLQSPDCSDSLEPITLGEGAFVEFRISEIYRRMQLLDNLYNSKSAWLDNQQMRERRAVSNFSTASQIYEAPKMLSMLFRTYKDQGQILYAATNQMFTSLSLREGRLVYYSKQHLTINMTVQETSTLNDGKWHNVSLFSESRSLRLIVDGRQVGDELDIAGVHDFLDPYLTILNVGGEAFVGCLANVTVNNELQPLNGSGSIFPEVRYHGKIESGCRGDIGQDAAQVADPLSIGFTLVIVFFVILVVAILGSYVIYRFRGKQEKIGSLSCGVPGFKIKHPGGPVTQSQVDHVLVRNLHPSEAPSPPVGAGDHMRPPVGSHHLVGPELLTKKFKEPTAEMPQPQQQQQRPQRPDIIERESPLIREDHHLPIPPLHPLPLEHASSVDMGSEYPEHYDLENASSIAPSDIDIVYHYKGYREAAGLRKYKASVPPVSAYTHHKHQNSGSQQQQQQHRHTAPFVTRNQGGQPPPPPTSASRTHQSTPLARLSPSSELSSQQPRILTLHDISGKPLQSALLATTSSSGGVGKDVHSNSERSLNSPVMSQLSGQSSSASRQKPGVPQQQAQQTSMGLTAEEIERLNGRPRTCSLISTLDAVSSSSEAPRVSSSALHMSLGGDVDAHSSTSTDESGNDSFTCSEIEYDNNSLSGDGKYSTSKSLLDGRSPVSRALSGGETSRNPPTTVVKTPPIPPHAYDGFESSFRGSLSTLVASDDDIANHLSGIYRKANGAASPSATTLGWEYLLNWGPSYENLMGVFKDIAELPDTNGPSQQQQQQTQVVSTLRMPSSNGPAAPEEYV

Target ORF information:

RefSeq Version	NM_058149.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fat (ft), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058149.4

ORF Insert Sequence:

ATGGAGAGGC TACTGCTCCT GTTTTTCCTG CTTCTGGCCG GCAGGGAATC CTTGTGCCAA 
ACAGGCGACA CCAAACTGGA GCTACTGGCA CCGCGTGGCC GAAGTTACGC CACCACCTAT 
GAACAATATG CCGCCTTTCC GCGAAGAAGA AGCTCATCAT CCTCGCCATC GGGTGAGATG 
CAATCCCGTG CGGTGGACAC CAGTGCCGAT TTCGAGGTGC TCGAGGGTCA GCCGCGTGGA 
ACTACGGTTG GTTTTATACC CACAAAACCA AAGTTCTCTT ATCGATTCAA TGAACCTCCT 
CGGGAATTCA CACTCGATCC GGTAACGGGT GAGGTGAAGA CCAATGTGGT GCTCGATCGG 
GAAGGTATGC GGGATCACTA CGATTTGGTG GTGCTCTCCT CACAGCCCAC CTATCCCATC 
GAGGTGCGGA TTAAGGTTCT GGATGTCAAC GACAATTCGC CGGAGTTTCC CGAACCCAGC 
ATCGCGATTT CCTTCTCCGA AAGTGCCACT TCGGGTACTC GATTGCTTCT CGATGCCGCC 
ACAGATGCGG ATGTTGGTGA AAACGGGGTG ACCGATCAGT ATGAGATCGT GGCTGGAAAT 
GTGGACAACA AATTTCGGCT TGTGACTACG GCGAATCCCA GTGGAGACAC CTCGTATCTG 
CATCTAGAAA CCACAGGAAA TCTCGACCGT GAGTCGCGTG GCAGTTACCA ACTAAACATC 
TCTGCCAGAG ACGGAGGATC TCCACCGCGT TTTGGCTACC TCCAAGTGAA CGTTACCATT 
CTGGATGTCA ATGATAATCC ACCGATCTTC GATCACAGCG ATTACAATGT GTCCCTAAAC 
GAAACAGCTC TCCCGGGAAC TCCGGTCGTG ACCGTCATGG CTTCGGACAA CGACTTGGGG 
GACAATAGCA AGATCACCTA CTATCTGGCC GAAACGGAGC ACCAATTCAC GGTGAATCCG 
GAGACGGGAG TCATTTCCAC CACGGAACGC GTAAACTGCC CCCAGCAAAC GAACGTGAAG 
AGCTCTGCTA GCCAGAAAAG CTGCGTTTTC ACTGTATTCG CCCGGGATCA TGGATCTCCA 
CGGCAGGATG GACGCACCTA TGTAACTGTT AATCTGCTGG ACACAAACGA TCACGACCCC 
ATCATCAGTT TCCGCTTCTT TCCGGATGGC GGAAAAGTGG CCACTGTGGA TGAGAATGCG 
GTAAATGGTA CTGTTGTGGC AGCCGTGGCC GTTAAGGATT CCGATTCCGG CCTAAATGGC 
AGAACATCGG TGCGAATTGT TTCCGGCAAT GAGCTGGGAC ACTTTCGCCT AGAAGAAGCT 
GCCGACTTGC ATATAGTAAG GGTAAATGGA GTCCTGGATC GCGAGGAGAT CGGGAAATAC 
AATCTGACCG TGGTGGCAAT GGATCAAGGA ACACCGGCGC GAACGACCAC GGCACATCTT 
ATCATCGACG TAAACGATGT GAATGATCAT GAACCGGTGT TCGAGAAATC AGAATATTCA 
GCGGTGCTGA GTGAACTGGC ACCCACGGGA AGTTTTGTGG CCTCAATTAC TGCCACGGAT 
GAGGATACTG GGGTCAATGC CCAGGTGCAC TATGATATTC TCTCGGGTAA TGAGCTCAAG 
TGGTTCTCCA TGGATCCACT GACAGGTTTG ATTGTGACTA CGGGTCCGTT GGATAGAGAG 
ATTCGCGACA CCGTAGAACT GAGCATCTCG GCCAGAGACG GTGGACCTAA TCCCAAATTT 
GCGTACACTC AGCTGAAGGT GATCATTCTG GACGAGAACG ATGAGGCGCC GCAGTTTAGC 
CAAAGAGAGC AGAATGTGAC TTTAGGTGAG GATGCTCCTC CGCAAACGAT TGTGGCTCTG 
ATGACGGCCA CTGATCATGA TCAGGGCACG AATGGATCGG TGACCTTTGC CCTAGCGCCC 
AGTGTGGAGC GCCTGTATCC GCTACAGTTT GCCCTCGATG CGTTGACGGG CCAATTAACA 
ACGCGAAGGC CTCTGGATCG TGAGAAAATG TCACAGTATG AGATCTCCGT GATTGCCCGC 
GATCAGGGAG CACCCACGCC CCAATCGGCC ACGGCCACCG TTTGGCTGAA TGTGGCCGAT 
GTAAACGATA ACGATCCACA GTTTTATCCC AGGCACTACA TCTACTCGCT CGCCGATGAC 
GATGATGATA TCAAGCTCAA GAAGGAGGTG GAGAAGGAGA GGATCCTGCT CCATGTCACG 
GCCTCAGATA AAGACGATGG CGATAATGCC TTAATTGAGT ACAGACTCGA GTCAGGTGGC 
GAGGGTCTCT TCCAGCTGGA CGCCCGATCG GGTGCCATCA GCCTGCGTGG TGATGCTCCT 
GCATCGATGC ATTGGAAGCC ACACTACAAG CTCCTCGTAT CGGCTCGTGA TGCTGGGCAA 
CGGCGGAGCC AGCAAGATGC CATTGTGGAA ATTGTGCTCA AGTCCAAGTT GGAGATGCTT 
GAATGCGGTC AGGCACAAGC TGGTGGTTAT GAGTTCCAGA TGGTCGAGGA TCACGAGCAG 
CAAAGGAACA GCCAACCAAA TCGCGAGGTG GGCATCGTGC AGGTAAAATC CACGAATGGC 
AAGGCGAACT CTCATATCGA ATATGATATC ATCCAAGGCG ACAGGGCTCA GAATTTCCGA 
ATCGATACCA GAAGTGGTCG CATCACCACC GCCCGACCTT TGGATCGCGA GGAGCAGGCC 
AATTATCGTT TGACTATCTT GGCCAGCTCC AGTTCCAGCT CCAGTGCAGC CGCCAGCAGC 
GTTTCATATG GCCAGTGCAT TGTTAATATA GCCATTATCG ATCTGAATGA TAATGCTCCC 
GTTTTCGCCT TGGATCGCGA GTCAGAGCCC ACAATCTCGC TGCCGGAGAA TGCGGCCGTG 
GGTCAGGAGA TCTACCTATC CAGGGTCAGA GATCGGGATG CGGGCGTGAA TAGTAGGATC 
AGCTATAGCC TCACCAACAA TCCCAATCAG CAGTTCCGCA TTGGACCGGT CACGGGAGTT 
CTCTACCTTC AGAGGCCCAT AAGAGCAGAG CCTGGATCCC TCATCCACGT GGAACTAATG 
GCCACGGATG CGGGTAGTCC TCCGCTCTCC AGTAAACTGA GTCTATCTGT GCTGATAGCC 
GATGTTAACG ATCACACTCC CGTCTTTGAT CACACATCCT ATGAGACTTC CCTCCCAGAA 
ACCACGAAAG TGAACACTCG ATTTTTCGCT TTGGCTGCTA CGGATATTGA TTTGGGGGAC 
AATGGACGGA TCTCGTACGA GATCATCGAG GGCAACACTG AGCGCATGTT CGGTGTCTTT 
CCCGATGGCT ATCTGTTTGT AAGAGCTCCA CTCGATCGTG AGGAGCGGGA TTACTATGCC 
CTAACTGTTT CTTGCCGGGA TGCTGGTCAA CCATCCAGAT CTTCGGTGGT TCCAGTTGTC 
ATCCATGTCA TCGATGAGAA CGACAATGCC CCACAGTTCA CGAACAGCAC ATTTACGTTT 
AGTATTCCGG AAAATGCTCC TGCTGACACG TTTGTGGGAA AGCTAACCGC AGTTGATCGG 
GATATCGGAA GAAATGCCGA GCTCAGTTTT ACCCTGTCCA GTCAGACGCA GGACTTTACG 
ATTGACACCA GGAATGGTTT CATAAAAACT CTGCGACCCT TCGATCGCGA GGCTCTGGTG 
AAAGTCAGCA GAAATGCGGA GGCAAGCGGA GAGGATGGTT CTCTCAGGGG TTCAATGGCT 
GGCAACTATA TGCTACTGGA GGCCACAGTT TCGGACAATG GCATTCCTCG CTTACAGGAC 
AAAGTCAAGG TGAAGGTCAT CGTCACCGAT GTCAACGATA ATGCACCGGA ATTCCTGAGA 
GCACCCTATC ATGTTACCAT TTCTGAGGGC GCCTCTGAGG GCACGCATAT AACGCATGTC 
TTTACCCAAG ATGCGGATGA GGGTCTGAAT GGAGATGTCT ACTACTCACT GGCGAAGGGA 
AATGAGGCTG GTCAGTTCAA TTTGGATAGC GCCACAGGAC AGCTTTCGTT GGGGCGCAGG 
TTGGACCGGG AGTCCCAGGA GATTCATCAC CTGATCGTCG TGGCCAAGGA TGCTGCTCTC 
AAGCATCCGC TGAGCTCTAA TGCTAGCATT ACAATTGTGG TTCTGGACGA GAACGACAAT 
GCTCCGGAGT TCACGCAGTC GAGCAGTGAG GTTTCCGTTT TGGAGACAAG TCCAACGGGC 
ACTGAATTGA TGAGATTCCG AGCTAGTGAT GCGGATCAAG GGGTAAATAG CCAGGTGGTC 
TTCAGTATAT CGGCTGGAAA TCGCCGTGAT ACCTTCCACA TCGACAGCAT TACGGGTAGC 
CTCTATCTGC ACAAGCCTCT GGACTACGAG GACATAACCA GCTATACTCT CAACATCACT 
GCCTCAGATT GTGGCACTCC GTCGTTGTCT ACCACCGTTT TGTATAACGT TCTCGTCGTG 
GATGACAACG ACAATCCGCC CATCTTTCCC AGCACTGCGA TTGTTCGGCA GATCAAGGAG 
GGAATTCCCC TGAAAACACC CATTGTGACC GTAACCGCCG ACGATCCGGA TTCGGGACTT 
AACGGCAAGG TTAGCTATGC CATTAGCAAG CAGGAACCCC AACTTCCACA GGGACGACAC 
TTTGGCATAA ACACGGAAAC GGGTGTGATT CACACGCTGA GGGAGATTGA TCGCGAATCC 
ATTGATACCT TTCGGCTAAC CGTGGTGGCC ACGGATCGTG CTCAGCCTTC GGAGCGCCAG 
CTCTCGACGG AAAAACTGGT CACCGTGATT GTGGAGGACA TAAACGATAA CGCACCAGTT 
TTTGTGTCCA TGAATGCGGC TATCCTGCCT CCAAAGTTCT CGACTTCCAA GGGCAGCAGC 
ACAGCGGTGA TGCAGGTTCA TGCCAAGGAT GCGGACTCGT CCAGCAACGG ATTGGTGACC 
TACGAGATTG TCAGTGGCCC CCAGGAGCTG TTCAAGCTGC AAAGGAACAC GGGTATCATA 
ACCTTTACCC CAGGTCCTCA GTTCAAGCAG GAGGTTCGCT ACCAGCTGAC CCTTAAGTCC 
ACCGACGAAG CCGTTCAAAG CGAACGTCGC AGCAGCGAGG TCTACATTAC AATCATAACG 
CCAGGCTCCG GCGGAAGTGA GTCCTCCGTT CCACAGTTCG AGCAACGGAG TAAGCTTTCC 
GGTTCCGTCT ACGAGAATGA ACCAATTGGA ACTAGTATTC TGACGGTGAC TGCTCATCTG 
GCGTCCGCCG AGATAGAATA CTTTGTGACG AATGTTACGG CAACCGGATC GCGTGGTCAG 
GTAGATCGCC TCTTCGACAT CGATGCCAAG CTGGGAATTC TGTCCACCGC CGCTGAACTG 
GACCGTGAAG CCGGACCAGA GGAGTACGAG GTGGAGGTAT ACGCCATCGC ACTTGGCGGA 
CAGCCACGTA CATCCCGAAC GAAGGTCAGA GTAACCGTTC TCGACAAGAA CGATAGTCCA 
CCGCAGTTCC TCGACACACC GTTCGTCTAC AATGTCAGCG AGGATCTACA GATTGGACAC 
ACCATCTCTA CGCTGCGAGC CCACGATCCG GATACGCTTG GTTCGGTGAC GTTCCTTCTG 
ATGGATGGGC ACGATGGCAA GTTCCTGCTG GAACCGTCCA CTGGAAAACT GATCCTCAAC 
GATACCCTCG ATCGGGAGAC AAAGAGCAAG TATGAGCTAA GGATTCGCGT ATCCGACGGA 
GTGCAGTACA CAGAGGCATA TGCCACTATA CAGGTGAGCG ATACCAATGA TAATCCACCC 
TTATTCGAGG ATACCGTGTA CTCCTTTGAC ATACCGGAAA ATGCCCAACG GGGCTACCAA 
GTGGGTCAGA TTGTGGCCAG GGATGCCGAT CTGGGCCAAA ATGCCCAATT GTCCTACGGC 
GTGGTCTCGG ATTGGGCCAA CGATGTCTTC AGCCTGAATC CACAAACAGG AATGCTGACC 
CTAACTGCGC GACTGGATTA CGAAGAGGTT CAGCACTATA TCCTGATTGT TCAGGCGCAG 
GATAATGGCC AACCCAGCTT GTCCACCACC ATAACGGTCT ACTGCAATGT CCTCGATCTG 
AACGATAATG CACCTATTTT CGATCCCATG AGCTATTCGA GCGAGGTGTT CGAGAATGTG 
CCCATCGCCA CAGAGGTAGT CACTGTGTCA GCAAAGGATA TAGATTCGGG CAACAATGGG 
CTCATTGAGT ACAGCATCAC CGCAGGAGAT GTCGACTCAG AGTTTGGAAT CGATAGCAAT 
GGCACTATAC GCACTCGCCG CAACTTGGAC AGGGAACACC GATCGACGTA CACACTTACG 
GTTACCGCCC GAGATTGTGC CGATGAATTT GCATCCTTCA GCGAACTGGA GGAGACGCAG 
CTGAAGCTCA AGTATCGATC GCCTAGAAAG TACCAACAAA CACGTCAAGA GTTTTTGGCC 
CATCAAAAGC AGCAGCGGCT CTCCTCGACT GTAAAGGTGA CGATACTCAT CAAAGATGTC 
AACGACGAAG TGCCCGTCTT CATTTCCGCA AACGAAACGG CGATCATGGA GAATGTGGCC 
ATCAATACGG TCGTTATCGC TGTCAAGGCT GTCGACAATG ACGAGGGCCG CAACGGATAC 
ATCGATTATC TGATGAAGGA GGCCAGAGAT GAGGACATGG GTCAATCGGA TCCGCTGCCC 
TTCTCCCTGA ATCCCACCGA TGGACAGTTG CGCGTAGTCG ATGCCTTGGA CCGTGAATTG 
CGCTCAAGTT ATTTGCTAAA CATCACAGCC CGAGATCGTG GAGAGCCACC CCAGTCCACG 
GAGTCGCAGT TGCTCATTCG AATTCTGGAC GAGAACGACA ATAGTCCCGT CTTCGATCCG 
AAGCAATACT CTGCCTCGGT GGCGGAAAAC GCCAGCATTG GTGCCATGGT CCTTCAGGTA 
TCCGCCACGG ATGTGGATGA AGGTGCAAAT GGACGCATCC GCTATTCCAT AGTGCTGGGC 
GATCAGAACC ATGACTTCAG CATCAGCGAG GATACGGGTG TGGTGCGGGT GGCTAAGAAC 
CTAAACTACG AACGACTTTC CCGATACTCG CTCACCGTAC GCGCCGAGGA TTGTGCCTTG 
GAGAATCCTG CTGGCGACAC TGCCGAGCTG ACCATCAATA TTCTGGACAT AAACGATAAT 
AGACCGACAT TTTTGGATTC CCCATATTTG GCACGTGTCA TGGAGAACAC TGTACCGCCA 
AATGGTGGAT ATGTGCTCAC TGTAAACGCC TATGATGCGG ACACTCCACC GCTTAATTCG 
CAGGTGCGCT ACTTTCTCAA GGAAGGCGAT AGTGATCTCT TCCGCATCAA TGCCTCTTCG 
GGTGACATCG CGCTGTTGAA ACCCCTGGAT CGAGAGCAGC AGAGCGAGTA CACCCTGACA 
CTGGTGGCCA TGGACACGGG TTCGCCTCCA CTTACGGGCA CGGGAATTGT GCGAGTGGAG 
GTGCAGGACA TTAATGACAA CGATCCGGTA TTCGAACTGC AATCCTATCA TGCCACTGTG 
CGCGAGAATT TACCGTCGGG AACTCATGTC CTGACCCCCA GAGCAACGGA TAAGGACGAA 
GGACTAAACG CCAAGCTGCG CTTTAATTTG TTGGGAGAGC ACATGCATCG GTTTCACATA 
GATTCAGAAA CGGGCGAGAT TAGCACAGCT ACAACTCTTG ATCGTGAAGA GACTTCCGTG 
TACCACTTGA CCTTAATGGC CCAGGATAGC TCGATTACCG AGCCACGAGC ATCGTCTGTA 
AATCTCACAA TCAGTGTGTC CGATGTCAAT GACAATATCC CGAAATTCGA TTCGACCACC 
TACAACGTAG CAGTTCCGGA GAGGATCAGC AAGGGTGAGT TTGTGTTTGG AGCTCGAGCC 
TTAGACTTGG ATGATGGCGA GAATGCTGTG GTACATTACA CGATTAGCGG GCGAGATCAA 
CATTACTTTG ATATCAACAC CAAAACGGGT GTGGTGAGCA CCAAGCTGGA GCTTAAGACC 
AAGACCAAGT CTCACGATGA TTTAACCTAT ACGATTGTGA TATCTGCAAT GGATCAGGGC 
GAGCAATCTC TTAGTTCCAA GGCTGAGCTT ACAGTTATCC TCCGACCACC GGAGCTCTTT 
CCCACCTTTG CCTACATGGC CAACAGTCAC TTTGCCATGT CAGAGGATGT TAGGCCGGGC 
AAGATGATAA CCAAAGTCAG TGCCACATCG CCCAAAAAGG GTTTGGTGGG TAAAATTCGC 
TATGCCATAG CCGGCGGAAT AATGGGGGAT TCTCTGAGAG TGGATCCCAA TAGCGGATTA 
CTGAGCGTTG GTCAGGATGG CCTGGACTAT GAGCTGACAC ATCTGTATGA GATTTGGATT 
GAGGCCGCCG ATGGAGACAC GCCAAGTCTG CGTAGTGTAA CTCTTATAAC GCTCAACGTA 
ACGGATGCCA ATGACAATGC TCCCGTTATG GAACAGCTCA TTTACAATGC CGAGGTCCTG 
GAGGAGGAGT CCCCACCGCA GTTGATAGCC GTTGTGAAAG CCAGCGATCG TGACTCCGGC 
GACAATGGAA ATGTCATCTA TCGCCTACAG AATGACTTCG ACGGCACATT TGAGATTACG 
GAATCGGGTG AGATTTACAC CAGGATGCGA CTGGATCGCG AGGAGATCGG CGATTATGCG 
TTCGTAGTGG AGGCCGTTGA CCAGGGTGTT CCTCATATGA CGGGAACAGC TAGTGTCCTA 
CTGCATCTAC TGGACAAGAA CGACAACCCG CCCAAGTTCA CACGCCTCTT TTCCCTGAAT 
GTAACCGAGA ACGCCGAGAT CGGTAGCTTT GTGATCCGAG TCACCTCTTC TGATTTGGAT 
CTGGGTGCAA ATGCCAATGC TTCTTACTCC TTCAGCGAGA ATCCCGGCGA GAAGTTTCGC 
ATTGAGCCGC AGAGTGGAAA CATCACAGTG GCCGGTCATC TTGATCGCGA GCAACAGGAT 
GAGTACATCC TGAAAGTGGT GGCCTCTGAT GGAGCCTGGC GTGCCGAGAC ACCCATTACC 
ATCACAATCC AGGACCAGAA TGACAATGCC CCCGAATTCG AGCATAGCTT CTACAGCTTC 
AGTTTCCCGG AGCTGCAGCA ATCGATTGCT TTGGTGGGCC AGATCATAGC CACGGATCGC 
GATAAACAGG GTCCCAACTC AGTGATCTCC TACTCGCTGC AGCAGCCGTC GCCCATGTTC 
AGCATAGATC CAGCCACGGG TGAGGTCTTC AGTAAGAAGG CGGTTCGCTT CAAGCACTCT 
CAGTACGTTC GCTCACCGGA GAATATGTAC GCCCTCACCG TTCTGGCCAC GGATAATGGT 
AAGCCTCCTC TGTACTCCGA GTGCCTGGTG AACATCAATA TCGTCGACGC CCACAACAAT 
CCACCAAAGT TCGAACAGGC GGAATATCTG GCGCCGCTGC CACAGGATGC AGTTCGTGGT 
CAGCGAATAG TTCGAGTCCA CGCAAATGAC AAGCAGGATC TGGGCACCAA CGAAATGGAC 
TACTCCCTGA TGACATTCAA TTTGAGTTCG ATCTTCAGTG TCGGCAGACA CGATGGTTGG 
ATTACTTTGG TCAAACCCAT TCAAGTGCCG CCAAACACTC GATATGAACT GGTTGTTAGG 
GCCACCGATC GTGGTGTTCC TCCCCAGTCC GATGAGACAC GAGTCGTGAT CGTGGTAACC 
GGGGAGAATA TGGACACACC CAGATTTTCC GTAAACAGCT ATCAAGTCAT TGTGCCCGAG 
AATGAGCCTG TTGGGTCCAC AATTCTAACT GTGGGCGCCA CTGACGATGA TACGGGTCCA 
AATGGAATGC TGCGCTACTC CATTTCCGGT GGCAATGAGC GTCAAGACTT CAGTGTGGAT 
GAACGTACGG GTGGCATAGT GATACAACAG CAATTGGATT ATGACCTTAT ACAGGAATAC 
CATTTGAATA TCACAGTTCA GGATCTGGGC TACCATCCAC TATCCTCAGT TGCAATGCTT 
ACGATCATTC TGACTGATGT TAATGACAAT CCACCTGTGT TCAATCACAA GGAATACCAC 
TGTTATATAC CTGAAAACAA ACCGGTTGGC ACCTTTGTAT TCCAGGCTCA TGCCGCGGAT 
AAGGATTCAC CGAAAAATGC TATTATCCAT TATGCATTCC TGCCCAGCGG ACCAGACAGG 
CACTTCTTCA TAATGAACCA GAGTAATGGC ACCATTTCTT CGGCAGTTTC CTTTGACTAT 
GAAGAGCGCC GCATCTACAC ACTACAGATA AAGGCGAAAA ATCCCGATTC GAGCATGGAG 
AGCTACGCCA ATCTCTATGT GCATGTCCTG GGCGTGAATG AGTTTTATCC ACAATTCCTG 
CAGCCCGTCT TTCACTTTGA CGTATCGGAA ACATCGGCGG TGGGCACTAG AGTGGGTGCC 
GTTCAGGCCA CCGATAAGGA CAGCGGCGAA GATGGACGTG TCTACTACCT TCTGGTGGGT 
TCCAGTAACG ACAAGGGTTT CCGCATCGAT ACCAATACGG GCTTGATTTA TGTAGCCCGA 
CACTTGGACA GGGAGACACA GAACCGTGTG GTACTCACAG TGATGGCCAA AAACTATGGC 
AGTATTCGTG GTAACGACAC TGACGAGGCA CAAGTGATTA TATCGATTCA GGATGGCAAC 
GATCCGCCGG AGTTCATAAA GCACTACTAT ACCTCCACGA TTTCCGAGGC AGCTCCGGTT 
GGCACGAAGG TCACAACGGT CAAGGCCATT GACAAGGATG TGCGCACACA AAATAATCAG 
TTTAGCTATT CGATCATTAA TGGCAACCTG AAACAAAGCT TTAAGATAGA TGTGCAAACG 
GGTGAGATTA GCACGGCAAG TCGTTTGGAT CGTGAGGAGA CATCCACCTA TAACTTGGTG 
ATTGGAGCCA TTGACACGGG ACTGCCTCCA CAGACGGGAA GTGCAACGGT GCATATCGAA 
CTAGAAGATG TGAACGATAA TGGACCCACA TTCACACCTG AGGGTCTGAA TGGTTATATC 
TCCGAGAATG AACCGGCTGG CACTTCCATT ATGACACTAA TAGCCAGTGA TCCCGATCTA 
CCGCGCAATG GAGGACCCTT CACCTATCAG TTGATTGGTG GTAAGCACAA GTCCTGGCTA 
AGTGTGGACA GAAACTCTGG TGTGGTTAGG TCCACAACCA GTTTCGATCG CGAAATGACA 
CCCATTCTAG AGGCCATAAT TGAAGTCGAG GACAGTGGAA AGCCCAAACA AAAGTCCCAG 
CACCTGCTCA CGATCACTGT ATTGGATCAG AACGATAATC CATCGACCAC GAGGAGCTTG 
CACATAGCAG TCAGTCTGTT CAACGGCGAT CTGCCTTCGA ATGTGAAGCT GGCCGATGTG 
CGTCCCAATG ATATTGACAT CGTGGGCGAT TATCGTTGTC GCCTCCAGAA GAATCCCGCC 
CAGAGTCAAC TGCAGTTGGC TATTCCTCGG GCCTGTGATC TGATCACCAC CTCACATACG 
ACGCCAATAG CTTCGGTTTT CAGCTATACG GGAAATGACG GAAAACATGG GGATGTTAGC 
TCCAAAGTTA GTGTCGCCTT CCAGAGTTTT AACAACGAAA CATTGGCCAA CTCAGTGTCC 
ATCATGGTAC GAAATATGAC AGCTTACCAC TTCTTGGCTA ATCACTATCG ACCAATTCTG 
GAGATGATTA AGTCTAGAAT GAGTAACGAG GATGAGGTCA TTCTATATAG TCTTCTAGAG 
GGTGGCAGTG GCAATTCCAC CAATCTCCAA CTGCTAATGG CAGTCAGATT GGCGAAGACC 
AGTTACCAAC AACCCAAATA TTTGATTGAA CGCTTGCGAG AGAAGCGTTC AGCGTTTTCG 
GAACTCCTGC AAAAGGAGGT GATTGTGGGC TATGAACCCT GCAGTGAACC GGATGTTTGT 
GAAAATGGCG GAGTCTGCAG TGCCACCATG CGACTGCTGG ATGCCCATAG CTTTGTTATC 
CAAGACAGTC CGGCCTTGGT GCTGAGTGGT CCTCGGGTTG TGCACGACTA CAGCTGCCAG 
TGCACCAGTG GATTCTCGGG CGAGCAGTGC AGTCGTCGGC AGGATCCTTG CCTGCCCAAT 
CCTTGCCATT CGCAGGTCCA ATGCCGTCGC CTGGGTAGCG ATTTCCAGTG CATGTGTCCT 
GCCAATCGGG ATGGCAAGCA CTGCGAGAAG GAACGCAGTG ACGTGTGCTA TAGCAAGCCG 
TGTCGCAATG GAGGAAGTTG CCAACGCAGT CCGGACGGAT CCTCCTACTT TTGCCTATGT 
CGTCCCGGAT TCCGTGGCAA TCAGTGCGAG AGCGTGTCGG ACTCATGCCG ACCCAATCCC 
TGTCTGCACG GTGGCTTGTG TGTTAGTCTC AAGCCAGGAT ACAAATGCAA CTGCACGCCG 
GGACGATATG GACGACATTG CGAGCGTTTT AGCTATGGAT TCCAACCGCT GTCCTACATG 
ACTTTTCCCG CTCTGGATGT GACCACCAAT GATATATCCA TTGTGTTCGC CACGACGAAA 
CCCAACTCCT TGTTGCTGTA CAACTACGGA ATGCAGAGTG GCGGCAGATC CGATTTCCTG 
GCCATCGAAC TGGTGCACGG CAGGGCGTAT TTCTCATCTG GAGGAGCTCG TACTGCTATC 
AGTACTGTGA TTGCTGGACG AAATCTGGCT GACGGTGGAT GGCACAAGGT TACGGCCACC 
CGGAATGGAC GTGTGATGTC ACTCAGCGTG GCCAAGTGCG CAGATTCCGG TGACGTTTGT 
ACGGAATGCC TGCCCGGAGA TTCTAGCTGC TATGCGGACG AAGTGGGACC AGTGGGAACT 
CTCAACTTCA ACAAACAGCC CCTGATGATC GGTGGCTTGT CCTCGGCTGA TCCTATCTTG 
GAGCGACCTG GTCAAGTGCA CAGTGATGAT CTGGTGGGCT GTCTGCACAG TGTTCATATC 
GGAGGACGTG CCCTGAACCT CAGCTTGCCG CTTCAGCAGA AGGGCATTTT AGCCGGATGC 
AATCGGCAGG CGTGTCAACC AGCTTTGGCC GCCGAACGCT GTGGAGGATT CGCTGGACAA 
TGCATCGATC GGTGGTCCAG TTCGCTATGT CAGTGCGGCG GACACTTACA GTCGCCCGAT 
TGCTCGGACT CCTTGGAACC CATCACCCTG GGCGAAGGTG CCTTTGTGGA GTTTAGAATT 
TCGGAAATAT ATCGTCGCAT GCAGCTGCTG GACAATCTGT ATAACAGCAA AAGTGCCTGG 
CTGGATAACC AACAGATGAG AGAACGTCGA GCCGTAAGCA ATTTCAGCAC AGCTTCCCAG 
ATCTACGAGG CTCCAAAGAT GCTTAGCATG CTGTTCCGCA CCTATAAGGA TCAGGGTCAG 
ATCCTTTATG CGGCCACCAA TCAAATGTTT ACCTCACTGT CCCTGCGCGA GGGTCGTCTG 
GTTTACTACT CCAAACAGCA TCTTACCATC AATATGACGG TGCAGGAAAC TTCTACGCTC 
AACGATGGCA AGTGGCACAA TGTGAGCTTG TTTAGCGAGA GTCGAAGTCT GCGACTCATT 
GTCGATGGAA GGCAAGTGGG TGATGAGCTG GATATTGCCG GGGTGCACGA CTTCCTCGAT 
CCCTATTTGA CCATTCTGAA TGTGGGTGGC GAAGCATTTG TTGGTTGCCT GGCCAATGTG 
ACGGTGAACA ACGAACTGCA GCCGCTAAAC GGATCAGGCA GCATCTTTCC GGAGGTTCGT 
TATCACGGCA AGATTGAGTC CGGCTGCAGA GGAGACATTG GCCAAGATGC CGCCCAGGTG 
GCCGATCCTC TCAGCATTGG CTTCACCCTG GTCATTGTCT TCTTCGTCAT TCTGGTGGTG 
GCTATACTGG GCTCCTATGT TATCTATCGA TTCCGCGGAA AGCAGGAGAA GATTGGTAGT 
TTGAGTTGCG GTGTGCCCGG CTTCAAGATT AAGCACCCTG GTGGTCCAGT GACGCAGAGT 
CAGGTGGATC ACGTCTTGGT TCGCAATCTG CATCCCAGTG AAGCCCCGTC ACCGCCAGTT 
GGAGCTGGAG ATCATATGCG ACCGCCAGTG GGTAGCCATC ACCTGGTGGG TCCCGAACTA 
TTGACCAAGA AGTTTAAGGA ACCCACTGCG GAGATGCCAC AGCCGCAGCA GCAGCAGCAA 
CGTCCCCAGC GACCCGATAT CATAGAGCGC GAGAGTCCTC TGATAAGGGA GGATCATCAC 
CTGCCCATTC CGCCGCTTCA TCCTCTCCCG CTGGAGCACG CCAGTTCCGT GGACATGGGT 
TCCGAGTACC CGGAACACTA CGACCTCGAG AACGCCAGCT CCATTGCTCC GTCCGACATT 
GATATAGTCT ATCATTACAA GGGCTATCGT GAGGCTGCTG GCCTACGCAA ATACAAGGCC 
AGTGTTCCAC CTGTTTCCGC CTACACGCAT CACAAGCATC AGAACTCTGG CTCTCAGCAG 
CAGCAGCAGC AGCACCGCCA CACAGCTCCC TTTGTGACCC GGAACCAAGG TGGCCAACCG 
CCACCGCCGC CCACCAGTGC ATCCCGCACC CATCAGAGCA CTCCACTGGC CCGACTCTCG 
CCCAGCTCGG AGTTGAGTTC CCAGCAGCCG CGCATTCTCA CTTTGCACGA CATTTCCGGA 
AAGCCGCTGC AAAGTGCCCT GCTGGCCACG ACCTCCAGTT CCGGTGGCGT TGGCAAGGAC 
GTTCACAGCA ACAGCGAACG CAGTCTGAAC AGCCCGGTTA TGTCGCAGCT GTCCGGCCAA 
AGTTCCAGTG CCAGCAGGCA AAAGCCCGGA GTGCCACAGC AGCAGGCGCA GCAAACTTCC 
ATGGGCTTGA CCGCCGAGGA GATTGAGAGA TTGAATGGCA GACCACGAAC TTGTAGCCTA 
ATCTCCACCC TGGATGCCGT CTCCTCCAGC AGTGAGGCGC CTCGAGTGTC GAGCAGCGCT 
CTGCATATGT CGCTGGGTGG AGATGTGGAT GCCCATAGTT CGACTTCCAC GGACGAAAGC 
GGCAACGACA GCTTCACGTG CTCGGAGATC GAGTACGACA ATAACAGTCT CAGTGGCGAC 
GGCAAGTATT CCACCAGCAA GAGTCTCCTC GATGGACGCA GTCCCGTGTC CAGAGCACTA 
AGCGGTGGTG AGACGAGCAG AAATCCACCG ACAACTGTGG TCAAGACACC GCCCATTCCG 
CCGCACGCCT ACGATGGATT CGAGTCATCA TTTCGCGGAT CGCTCAGCAC GCTCGTGGCC 
AGCGACGATG ATATAGCCAA TCACCTGAGC GGTATCTACC GCAAGGCCAA TGGAGCCGCA 
TCCCCATCGG CCACCACCCT CGGCTGGGAG TATCTGCTCA ATTGGGGACC TAGCTACGAA 
AATCTGATGG GCGTCTTCAA GGACATTGCC GAGCTGCCGG ACACAAATGG ACCGTCGCAG 
CAACAGCAGC AGCAAACCCA AGTGGTGTCC ACGCTACGAA TGCCATCATC GAATGGACCG 
GCGGCTCCAG AGGAGTACGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ft cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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