bowl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bowl
Entrez Gene ID	33602
Full Name	brother of odd with entrails limited
Synonyms	17-29-5,BOWL,Bowl,CG10021,Dmel\CG10021,Su(tor)2-1,l(2)c,org2
General protein information	Preferred Names brother of odd with entrails limited Names CG10021 gene product from transcript CG10021-RB CG10021-PA CG10021-PB CG10021-PC CG10021-PD CG10021-PF CG10021-PG CG10021-PH bowel bowl-PA bowl-PB bowl-PC bowl-PD bowl-PF bowl-PG bowl-PH odd skipped-related 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 24C3-24C4\|2-16 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057535.4	NP_476883.1	brother of odd with entrails limited, isoform C
NM_164558.2	NP_722941.1	brother of odd with entrails limited, isoform D
NM_001258934.1	NP_001245863.1	brother of odd with entrails limited, isoform H
NM_164557.3	NP_722940.1	brother of odd with entrails limited, isoform B
NM_001258933.2	NP_001245862.1	brother of odd with entrails limited, isoform G
NM_164556.2	NP_722939.1	brother of odd with entrails limited, isoform A
NM_001258932.2	NP_001245861.1	brother of odd with entrails limited, isoform F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we find that C15 interacts physically with the Dll activator through contacts between their homeodomain and binds competitively with Dll to adjacent cognate sites on LAE, adding potential new layers of regulation by C15. Lastly, we show that C15 and Bowl activities regulate also rn expression
Title: Tissue-specific enhancer repression through molecular integration of cell signaling inputs.

Lines acts antagonistically to bowl in hub specification, establishing bowl as a positively acting factor in the development of the testis niche.
Title: lines and bowl affect the specification of cyst stem cells and niche cells in the Drosophila testis.

Together, lines and bowl act as a binary switch to generate a stable Notch signaling interface between Dl-expressing cells and adjacent distal cell.
Title: Essential roles for lines in mediating leg and antennal proximodistal patterning and generating a stable Notch signaling interface at segment borders.

Reciprocal roles are reported for bowl and lin in specifying the peripodial epithelium and the disc proper of the wing.
Title: Reciprocal roles for bowl and lines in specifying the peripodial epithelium and the disc proper of the Drosophila wing primordium.

Bowl is part of a genetic program that suppresses the formation of supernumerary appendages specifically in the fly's head.
Title: An antennal-specific role for bowl in repressing supernumerary appendage development in Drosophila.

The following bowl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bowl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13682	NM_164557.3 Latest version!	Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13682	NM_164556.2 Latest version!	Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13682	NM_001258932.2 Latest version!	Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13682	NM_001258933.2 Latest version!	Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13682	NM_057535.4 Latest version!	Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13682	NM_164558.2 Latest version!	Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13682	NM_001258934.1 Latest version!	Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bowl ( NM_057535.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476883.1 Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa13682
Clone ID Related Accession (Same CDS sequence)	NM_057535.4 , NM_164558.2 , NM_001258934.1 , NM_164557.3 , NM_001258933.2 , NM_164556.2 , NM_001258932.2
Accession Version	NM_057535.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brother of odd with entrails limited, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057535.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCACGG AAAGCTCATC CAGCGAGATC TCCGGCGGAG GAGGCGGTGC GATACCCATG 
CTGCGACCTT CCCGGATGGA TCAGTTCATG AACTCGATGG CGGCGGCAGC GGCGGCGGTG 
GGTGGTGGCG GTCTTCCTGG TGCGGCGGAT AGGAACGGCG GATCCGGAGG ATCGGATGGT 
GGCTCCCAGA ATGGCAACGG CGATTCGCGC AACTCATCGG CCAGCCGGAT CTCAGCGTAT 
GAAACACAGC TGGCCTATCA GCAGCATTTG GCCGGACTGC ATGGTCCGCC GCCACCGCCA 
CCACCATCGC ATCATCGCGA GATCTCTGCC TTTGTGCCGG TTCTGCCCAC CGGAAAGGTG 
CGCCCGGGTA GTAATTCCAA TTACGAAATC ATTGCCATGA TGGCGGACAA GCGAAAGGAG 
TTGGCCCTAA GGGAGGCGGC TGCGGCGGCG GCAATGTTAG GACGAGGTCC TGGAGGACCC 
GGCGGTCCTG GTGTGCCACC GCCTGGAGTG TTGTACGGAC CAGCTGGCGT GCCACCTCCG 
CCATATCTCA CCGGACCGGG TCCATCGCCC ACGGGAGCCG GAAGTTTTCC ATTTCCACCA 
GGAGCCGCCG CCGCAGCTCT ATTTCCACCT GGCCTGGGTC CTGGAATGCA TGCCGGACTC 
GATAGGCGCC TGCTACGTGC TCCTGGTCGG GCATCGCGGC CTAAAAAACA GTTCATCTGC 
AAGTTCTGCA ATCGACAATT CACCAAGTCG TACAATCTGC TCATCCACGA GAGGACACAC 
ACGGACGAGC GGCCCTATTC CTGTGATATC TGCGGCAAGG CGTTCCGGCG ACAGGATCAC 
CTGAGGGATC ACAGGTATAT TCACTCCAAG GAGAAACCCT TCAAATGCAC GGAATGCGGC 
AAGGGATTCT GCCAATCGAG AACCTTGGCT GTCCACAAGA TCCTGCACAT GGAGGAATCA 
CCCCACAAGT GCCCCGTCTG CAGTCGATCA TTCAATCAGC GCTCCAACCT GAAGACCCAT 
CTGCTCACCC ACACGGATCA CAAGCCCTAC GAGTGCTCTT CATGCGGCAA AGTTTTCCGC 
CGTAACTGCG ATCTACGACG CCATGCCTTG ACCCATGCAG TGGGTGAGGT CAACTCCGGG 
GACTATGTGG ATGTGGGCGA AGAGGATGAG GCCAGAAATT TAAGTGGCGA CGAGGAGGAT 
TCGTTGCTGG AAGTGGACTC GCCTCGCCAG TCGCCAGTTC ACAATTTGGG CGAGTCTGGA 
GGATCGGGTG AGAAATCTGA GTCCGAAAGA ATGAGACTCA AGCGCAAGGC AGCCATCGAT 
CATGAGGAAA GCGAAGAGGA GTTCGATGAC TTCGACGAGG AAGAGGAACT GCAGGATCTG 
CCAAGAGTAC ACGATTTGCC CAGGGAAGAG GACGACGACT TTGATCCCGA GGACGAGGAG 
CAGGCAGAAG TGGCACTAGT GGCGCGTTTC CAGGCGAGTA AGGCTGCCGC CACATCGCAA 
TCATCGTCGT CAGTGGGCAC CAAGCCGGAA CGCCAGGGAG TCACCCACTG TCATCATGAG 
GGTGGTGAAA CCTACACAAT GCGGCCACAT GGCGAGAAAC ATCAGGAGGA ACCCGGGAAC 
TCTGGCATTG CCAGCCTACC CGTTCCACCT TCCTTTGTGC GATATTCTGT ACCACCTGGG 
GCAGCTGGAC CGCCGCCTGC ACCACCTGGA GCTCCTCCGC CAACCCACCA GCACCCAGGA 
CATCCACACT TACTCCCACC CAACGGGGAT CCCTATTTGC CCATTCTGCA CGTTCGACGC 
GACTTGCACC ACAAGAGCTT GAACCTTTCG AAGGCTGGTG TTCCACCGCC ACCACATACA 
CCACCCACTA TAATAACCCA GCCGGAGTCG GGTAAACCAC CCAATCAACC TTTGCATTCA 
CCGCATGAAG CTATGCCCAG CTTCCTGGGA TCGATACCCA TGCGAAAGAG GATTCTTCCG 
GCACCGACCT TGGACTTGAT GGATCCGCAC CACCATCCTG GATTGGGTCA AAGGACCTTT 
GTGGATAGTC CCAGCATATA TGCTTTGAAT ATGTCGCGAC ATCCCCCCAG ACAGCTTCTG 
GGAAAACCAC CGAGTACGGA AACAAGTGGA GCCACCACCG AAAAGGGACC ACCAGTGGCA 
GCACCGCCCA TAGCACCACC GCCTGCACCA CCGAGAAGAA CAGGGTTCAG CATTGAGGAC 
ATAATGAGGC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476883.1
CDS	353..2587
Translation	MPTESSSSEISGGGGGAIPMLRPSRMDQFMNSMAAAAAAVGGGGLPGAADRNGGSGGSDGGSQNGNGDSRNSSASRISAYETQLAYQQHLAGLHGPPPPPPPSHHREISAFVPVLPTGKVRPGSNSNYEIIAMMADKRKELALREAAAAAAMLGRGPGGPGGPGVPPPGVLYGPAGVPPPPYLTGPGPSPTGAGSFPFPPGAAAAALFPPGLGPGMHAGLDRRLLRAPGRASRPKKQFICKFCNRQFTKSYNLLIHERTHTDERPYSCDICGKAFRRQDHLRDHRYIHSKEKPFKCTECGKGFCQSRTLAVHKILHMEESPHKCPVCSRSFNQRSNLKTHLLTHTDHKPYECSSCGKVFRRNCDLRRHALTHAVGEVNSGDYVDVGEEDEARNLSGDEEDSLLEVDSPRQSPVHNLGESGGSGEKSESERMRLKRKAAIDHEESEEEFDDFDEEEELQDLPRVHDLPREEDDDFDPEDEEQAEVALVARFQASKAAATSQSSSSVGTKPERQGVTHCHHEGGETYTMRPHGEKHQEEPGNSGIASLPVPPSFVRYSVPPGAAGPPPAPPGAPPPTHQHPGHPHLLPPNGDPYLPILHVRRDLHHKSLNLSKAGVPPPPHTPPTIITQPESGKPPNQPLHSPHEAMPSFLGSIPMRKRILPAPTLDLMDPHHHPGLGQRTFVDSPSIYALNMSRHPPRQLLGKPPSTETSGATTEKGPPVAAPPIAPPPAPPRRTGFSIEDIMRR

Target ORF information:

RefSeq Version	NM_057535.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057535.4

ORF Insert Sequence:

ATGCCCACGG AAAGCTCATC CAGCGAGATC TCCGGCGGAG GAGGCGGTGC GATACCCATG 
CTGCGACCTT CCCGGATGGA TCAGTTCATG AACTCGATGG CGGCGGCAGC GGCGGCGGTG 
GGTGGTGGCG GTCTTCCTGG TGCGGCGGAT AGGAACGGCG GATCCGGAGG ATCGGATGGT 
GGCTCCCAGA ATGGCAACGG CGATTCGCGC AACTCATCGG CCAGCCGGAT CTCAGCGTAT 
GAAACACAGC TGGCCTATCA GCAGCATTTG GCCGGACTGC ATGGTCCGCC GCCACCGCCA 
CCACCATCGC ATCATCGCGA GATCTCTGCC TTTGTGCCGG TTCTGCCCAC CGGAAAGGTG 
CGCCCGGGTA GTAATTCCAA TTACGAAATC ATTGCCATGA TGGCGGACAA GCGAAAGGAG 
TTGGCCCTAA GGGAGGCGGC TGCGGCGGCG GCAATGTTAG GACGAGGTCC TGGAGGACCC 
GGCGGTCCTG GTGTGCCACC GCCTGGAGTG TTGTACGGAC CAGCTGGCGT GCCACCTCCG 
CCATATCTCA CCGGACCGGG TCCATCGCCC ACGGGAGCCG GAAGTTTTCC ATTTCCACCA 
GGAGCCGCCG CCGCAGCTCT ATTTCCACCT GGCCTGGGTC CTGGAATGCA TGCCGGACTC 
GATAGGCGCC TGCTACGTGC TCCTGGTCGG GCATCGCGGC CTAAAAAACA GTTCATCTGC 
AAGTTCTGCA ATCGACAATT CACCAAGTCG TACAATCTGC TCATCCACGA GAGGACACAC 
ACGGACGAGC GGCCCTATTC CTGTGATATC TGCGGCAAGG CGTTCCGGCG ACAGGATCAC 
CTGAGGGATC ACAGGTATAT TCACTCCAAG GAGAAACCCT TCAAATGCAC GGAATGCGGC 
AAGGGATTCT GCCAATCGAG AACCTTGGCT GTCCACAAGA TCCTGCACAT GGAGGAATCA 
CCCCACAAGT GCCCCGTCTG CAGTCGATCA TTCAATCAGC GCTCCAACCT GAAGACCCAT 
CTGCTCACCC ACACGGATCA CAAGCCCTAC GAGTGCTCTT CATGCGGCAA AGTTTTCCGC 
CGTAACTGCG ATCTACGACG CCATGCCTTG ACCCATGCAG TGGGTGAGGT CAACTCCGGG 
GACTATGTGG ATGTGGGCGA AGAGGATGAG GCCAGAAATT TAAGTGGCGA CGAGGAGGAT 
TCGTTGCTGG AAGTGGACTC GCCTCGCCAG TCGCCAGTTC ACAATTTGGG CGAGTCTGGA 
GGATCGGGTG AGAAATCTGA GTCCGAAAGA ATGAGACTCA AGCGCAAGGC AGCCATCGAT 
CATGAGGAAA GCGAAGAGGA GTTCGATGAC TTCGACGAGG AAGAGGAACT GCAGGATCTG 
CCAAGAGTAC ACGATTTGCC CAGGGAAGAG GACGACGACT TTGATCCCGA GGACGAGGAG 
CAGGCAGAAG TGGCACTAGT GGCGCGTTTC CAGGCGAGTA AGGCTGCCGC CACATCGCAA 
TCATCGTCGT CAGTGGGCAC CAAGCCGGAA CGCCAGGGAG TCACCCACTG TCATCATGAG 
GGTGGTGAAA CCTACACAAT GCGGCCACAT GGCGAGAAAC ATCAGGAGGA ACCCGGGAAC 
TCTGGCATTG CCAGCCTACC CGTTCCACCT TCCTTTGTGC GATATTCTGT ACCACCTGGG 
GCAGCTGGAC CGCCGCCTGC ACCACCTGGA GCTCCTCCGC CAACCCACCA GCACCCAGGA 
CATCCACACT TACTCCCACC CAACGGGGAT CCCTATTTGC CCATTCTGCA CGTTCGACGC 
GACTTGCACC ACAAGAGCTT GAACCTTTCG AAGGCTGGTG TTCCACCGCC ACCACATACA 
CCACCCACTA TAATAACCCA GCCGGAGTCG GGTAAACCAC CCAATCAACC TTTGCATTCA 
CCGCATGAAG CTATGCCCAG CTTCCTGGGA TCGATACCCA TGCGAAAGAG GATTCTTCCG 
GCACCGACCT TGGACTTGAT GGATCCGCAC CACCATCCTG GATTGGGTCA AAGGACCTTT 
GTGGATAGTC CCAGCATATA TGCTTTGAAT ATGTCGCGAC ATCCCCCCAG ACAGCTTCTG 
GGAAAACCAC CGAGTACGGA AACAAGTGGA GCCACCACCG AAAAGGGACC ACCAGTGGCA 
GCACCGCCCA TAGCACCACC GCCTGCACCA CCGAGAAGAA CAGGGTTCAG CATTGAGGAC 
ATAATGAGGC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bowl ( NM_164558.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722941.1 Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa13682
Clone ID Related Accession (Same CDS sequence)	NM_057535.4 , NM_164558.2 , NM_001258934.1 , NM_164557.3 , NM_001258933.2 , NM_164556.2 , NM_001258932.2
Accession Version	NM_164558.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brother of odd with entrails limited, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_164558.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCACGG AAAGCTCATC CAGCGAGATC TCCGGCGGAG GAGGCGGTGC GATACCCATG 
CTGCGACCTT CCCGGATGGA TCAGTTCATG AACTCGATGG CGGCGGCAGC GGCGGCGGTG 
GGTGGTGGCG GTCTTCCTGG TGCGGCGGAT AGGAACGGCG GATCCGGAGG ATCGGATGGT 
GGCTCCCAGA ATGGCAACGG CGATTCGCGC AACTCATCGG CCAGCCGGAT CTCAGCGTAT 
GAAACACAGC TGGCCTATCA GCAGCATTTG GCCGGACTGC ATGGTCCGCC GCCACCGCCA 
CCACCATCGC ATCATCGCGA GATCTCTGCC TTTGTGCCGG TTCTGCCCAC CGGAAAGGTG 
CGCCCGGGTA GTAATTCCAA TTACGAAATC ATTGCCATGA TGGCGGACAA GCGAAAGGAG 
TTGGCCCTAA GGGAGGCGGC TGCGGCGGCG GCAATGTTAG GACGAGGTCC TGGAGGACCC 
GGCGGTCCTG GTGTGCCACC GCCTGGAGTG TTGTACGGAC CAGCTGGCGT GCCACCTCCG 
CCATATCTCA CCGGACCGGG TCCATCGCCC ACGGGAGCCG GAAGTTTTCC ATTTCCACCA 
GGAGCCGCCG CCGCAGCTCT ATTTCCACCT GGCCTGGGTC CTGGAATGCA TGCCGGACTC 
GATAGGCGCC TGCTACGTGC TCCTGGTCGG GCATCGCGGC CTAAAAAACA GTTCATCTGC 
AAGTTCTGCA ATCGACAATT CACCAAGTCG TACAATCTGC TCATCCACGA GAGGACACAC 
ACGGACGAGC GGCCCTATTC CTGTGATATC TGCGGCAAGG CGTTCCGGCG ACAGGATCAC 
CTGAGGGATC ACAGGTATAT TCACTCCAAG GAGAAACCCT TCAAATGCAC GGAATGCGGC 
AAGGGATTCT GCCAATCGAG AACCTTGGCT GTCCACAAGA TCCTGCACAT GGAGGAATCA 
CCCCACAAGT GCCCCGTCTG CAGTCGATCA TTCAATCAGC GCTCCAACCT GAAGACCCAT 
CTGCTCACCC ACACGGATCA CAAGCCCTAC GAGTGCTCTT CATGCGGCAA AGTTTTCCGC 
CGTAACTGCG ATCTACGACG CCATGCCTTG ACCCATGCAG TGGGTGAGGT CAACTCCGGG 
GACTATGTGG ATGTGGGCGA AGAGGATGAG GCCAGAAATT TAAGTGGCGA CGAGGAGGAT 
TCGTTGCTGG AAGTGGACTC GCCTCGCCAG TCGCCAGTTC ACAATTTGGG CGAGTCTGGA 
GGATCGGGTG AGAAATCTGA GTCCGAAAGA ATGAGACTCA AGCGCAAGGC AGCCATCGAT 
CATGAGGAAA GCGAAGAGGA GTTCGATGAC TTCGACGAGG AAGAGGAACT GCAGGATCTG 
CCAAGAGTAC ACGATTTGCC CAGGGAAGAG GACGACGACT TTGATCCCGA GGACGAGGAG 
CAGGCAGAAG TGGCACTAGT GGCGCGTTTC CAGGCGAGTA AGGCTGCCGC CACATCGCAA 
TCATCGTCGT CAGTGGGCAC CAAGCCGGAA CGCCAGGGAG TCACCCACTG TCATCATGAG 
GGTGGTGAAA CCTACACAAT GCGGCCACAT GGCGAGAAAC ATCAGGAGGA ACCCGGGAAC 
TCTGGCATTG CCAGCCTACC CGTTCCACCT TCCTTTGTGC GATATTCTGT ACCACCTGGG 
GCAGCTGGAC CGCCGCCTGC ACCACCTGGA GCTCCTCCGC CAACCCACCA GCACCCAGGA 
CATCCACACT TACTCCCACC CAACGGGGAT CCCTATTTGC CCATTCTGCA CGTTCGACGC 
GACTTGCACC ACAAGAGCTT GAACCTTTCG AAGGCTGGTG TTCCACCGCC ACCACATACA 
CCACCCACTA TAATAACCCA GCCGGAGTCG GGTAAACCAC CCAATCAACC TTTGCATTCA 
CCGCATGAAG CTATGCCCAG CTTCCTGGGA TCGATACCCA TGCGAAAGAG GATTCTTCCG 
GCACCGACCT TGGACTTGAT GGATCCGCAC CACCATCCTG GATTGGGTCA AAGGACCTTT 
GTGGATAGTC CCAGCATATA TGCTTTGAAT ATGTCGCGAC ATCCCCCCAG ACAGCTTCTG 
GGAAAACCAC CGAGTACGGA AACAAGTGGA GCCACCACCG AAAAGGGACC ACCAGTGGCA 
GCACCGCCCA TAGCACCACC GCCTGCACCA CCGAGAAGAA CAGGGTTCAG CATTGAGGAC 
ATAATGAGGC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722941.1
CDS	559..2793
Translation	MPTESSSSEISGGGGGAIPMLRPSRMDQFMNSMAAAAAAVGGGGLPGAADRNGGSGGSDGGSQNGNGDSRNSSASRISAYETQLAYQQHLAGLHGPPPPPPPSHHREISAFVPVLPTGKVRPGSNSNYEIIAMMADKRKELALREAAAAAAMLGRGPGGPGGPGVPPPGVLYGPAGVPPPPYLTGPGPSPTGAGSFPFPPGAAAAALFPPGLGPGMHAGLDRRLLRAPGRASRPKKQFICKFCNRQFTKSYNLLIHERTHTDERPYSCDICGKAFRRQDHLRDHRYIHSKEKPFKCTECGKGFCQSRTLAVHKILHMEESPHKCPVCSRSFNQRSNLKTHLLTHTDHKPYECSSCGKVFRRNCDLRRHALTHAVGEVNSGDYVDVGEEDEARNLSGDEEDSLLEVDSPRQSPVHNLGESGGSGEKSESERMRLKRKAAIDHEESEEEFDDFDEEEELQDLPRVHDLPREEDDDFDPEDEEQAEVALVARFQASKAAATSQSSSSVGTKPERQGVTHCHHEGGETYTMRPHGEKHQEEPGNSGIASLPVPPSFVRYSVPPGAAGPPPAPPGAPPPTHQHPGHPHLLPPNGDPYLPILHVRRDLHHKSLNLSKAGVPPPPHTPPTIITQPESGKPPNQPLHSPHEAMPSFLGSIPMRKRILPAPTLDLMDPHHHPGLGQRTFVDSPSIYALNMSRHPPRQLLGKPPSTETSGATTEKGPPVAAPPIAPPPAPPRRTGFSIEDIMRR

Target ORF information:

RefSeq Version	NM_164558.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164558.2

ORF Insert Sequence:

ATGCCCACGG AAAGCTCATC CAGCGAGATC TCCGGCGGAG GAGGCGGTGC GATACCCATG 
CTGCGACCTT CCCGGATGGA TCAGTTCATG AACTCGATGG CGGCGGCAGC GGCGGCGGTG 
GGTGGTGGCG GTCTTCCTGG TGCGGCGGAT AGGAACGGCG GATCCGGAGG ATCGGATGGT 
GGCTCCCAGA ATGGCAACGG CGATTCGCGC AACTCATCGG CCAGCCGGAT CTCAGCGTAT 
GAAACACAGC TGGCCTATCA GCAGCATTTG GCCGGACTGC ATGGTCCGCC GCCACCGCCA 
CCACCATCGC ATCATCGCGA GATCTCTGCC TTTGTGCCGG TTCTGCCCAC CGGAAAGGTG 
CGCCCGGGTA GTAATTCCAA TTACGAAATC ATTGCCATGA TGGCGGACAA GCGAAAGGAG 
TTGGCCCTAA GGGAGGCGGC TGCGGCGGCG GCAATGTTAG GACGAGGTCC TGGAGGACCC 
GGCGGTCCTG GTGTGCCACC GCCTGGAGTG TTGTACGGAC CAGCTGGCGT GCCACCTCCG 
CCATATCTCA CCGGACCGGG TCCATCGCCC ACGGGAGCCG GAAGTTTTCC ATTTCCACCA 
GGAGCCGCCG CCGCAGCTCT ATTTCCACCT GGCCTGGGTC CTGGAATGCA TGCCGGACTC 
GATAGGCGCC TGCTACGTGC TCCTGGTCGG GCATCGCGGC CTAAAAAACA GTTCATCTGC 
AAGTTCTGCA ATCGACAATT CACCAAGTCG TACAATCTGC TCATCCACGA GAGGACACAC 
ACGGACGAGC GGCCCTATTC CTGTGATATC TGCGGCAAGG CGTTCCGGCG ACAGGATCAC 
CTGAGGGATC ACAGGTATAT TCACTCCAAG GAGAAACCCT TCAAATGCAC GGAATGCGGC 
AAGGGATTCT GCCAATCGAG AACCTTGGCT GTCCACAAGA TCCTGCACAT GGAGGAATCA 
CCCCACAAGT GCCCCGTCTG CAGTCGATCA TTCAATCAGC GCTCCAACCT GAAGACCCAT 
CTGCTCACCC ACACGGATCA CAAGCCCTAC GAGTGCTCTT CATGCGGCAA AGTTTTCCGC 
CGTAACTGCG ATCTACGACG CCATGCCTTG ACCCATGCAG TGGGTGAGGT CAACTCCGGG 
GACTATGTGG ATGTGGGCGA AGAGGATGAG GCCAGAAATT TAAGTGGCGA CGAGGAGGAT 
TCGTTGCTGG AAGTGGACTC GCCTCGCCAG TCGCCAGTTC ACAATTTGGG CGAGTCTGGA 
GGATCGGGTG AGAAATCTGA GTCCGAAAGA ATGAGACTCA AGCGCAAGGC AGCCATCGAT 
CATGAGGAAA GCGAAGAGGA GTTCGATGAC TTCGACGAGG AAGAGGAACT GCAGGATCTG 
CCAAGAGTAC ACGATTTGCC CAGGGAAGAG GACGACGACT TTGATCCCGA GGACGAGGAG 
CAGGCAGAAG TGGCACTAGT GGCGCGTTTC CAGGCGAGTA AGGCTGCCGC CACATCGCAA 
TCATCGTCGT CAGTGGGCAC CAAGCCGGAA CGCCAGGGAG TCACCCACTG TCATCATGAG 
GGTGGTGAAA CCTACACAAT GCGGCCACAT GGCGAGAAAC ATCAGGAGGA ACCCGGGAAC 
TCTGGCATTG CCAGCCTACC CGTTCCACCT TCCTTTGTGC GATATTCTGT ACCACCTGGG 
GCAGCTGGAC CGCCGCCTGC ACCACCTGGA GCTCCTCCGC CAACCCACCA GCACCCAGGA 
CATCCACACT TACTCCCACC CAACGGGGAT CCCTATTTGC CCATTCTGCA CGTTCGACGC 
GACTTGCACC ACAAGAGCTT GAACCTTTCG AAGGCTGGTG TTCCACCGCC ACCACATACA 
CCACCCACTA TAATAACCCA GCCGGAGTCG GGTAAACCAC CCAATCAACC TTTGCATTCA 
CCGCATGAAG CTATGCCCAG CTTCCTGGGA TCGATACCCA TGCGAAAGAG GATTCTTCCG 
GCACCGACCT TGGACTTGAT GGATCCGCAC CACCATCCTG GATTGGGTCA AAGGACCTTT 
GTGGATAGTC CCAGCATATA TGCTTTGAAT ATGTCGCGAC ATCCCCCCAG ACAGCTTCTG 
GGAAAACCAC CGAGTACGGA AACAAGTGGA GCCACCACCG AAAAGGGACC ACCAGTGGCA 
GCACCGCCCA TAGCACCACC GCCTGCACCA CCGAGAAGAA CAGGGTTCAG CATTGAGGAC 
ATAATGAGGC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bowl ( NM_001258934.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245863.1 Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa13682
Clone ID Related Accession (Same CDS sequence)	NM_057535.4 , NM_164558.2 , NM_001258934.1 , NM_164557.3 , NM_001258933.2 , NM_164556.2 , NM_001258932.2
Accession Version	NM_001258934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brother of odd with entrails limited, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCACGG AAAGCTCATC CAGCGAGATC TCCGGCGGAG GAGGCGGTGC GATACCCATG 
CTGCGACCTT CCCGGATGGA TCAGTTCATG AACTCGATGG CGGCGGCAGC GGCGGCGGTG 
GGTGGTGGCG GTCTTCCTGG TGCGGCGGAT AGGAACGGCG GATCCGGAGG ATCGGATGGT 
GGCTCCCAGA ATGGCAACGG CGATTCGCGC AACTCATCGG CCAGCCGGAT CTCAGCGTAT 
GAAACACAGC TGGCCTATCA GCAGCATTTG GCCGGACTGC ATGGTCCGCC GCCACCGCCA 
CCACCATCGC ATCATCGCGA GATCTCTGCC TTTGTGCCGG TTCTGCCCAC CGGAAAGGTG 
CGCCCGGGTA GTAATTCCAA TTACGAAATC ATTGCCATGA TGGCGGACAA GCGAAAGGAG 
TTGGCCCTAA GGGAGGCGGC TGCGGCGGCG GCAATGTTAG GACGAGGTCC TGGAGGACCC 
GGCGGTCCTG GTGTGCCACC GCCTGGAGTG TTGTACGGAC CAGCTGGCGT GCCACCTCCG 
CCATATCTCA CCGGACCGGG TCCATCGCCC ACGGGAGCCG GAAGTTTTCC ATTTCCACCA 
GGAGCCGCCG CCGCAGCTCT ATTTCCACCT GGCCTGGGTC CTGGAATGCA TGCCGGACTC 
GATAGGCGCC TGCTACGTGC TCCTGGTCGG GCATCGCGGC CTAAAAAACA GTTCATCTGC 
AAGTTCTGCA ATCGACAATT CACCAAGTCG TACAATCTGC TCATCCACGA GAGGACACAC 
ACGGACGAGC GGCCCTATTC CTGTGATATC TGCGGCAAGG CGTTCCGGCG ACAGGATCAC 
CTGAGGGATC ACAGGTATAT TCACTCCAAG GAGAAACCCT TCAAATGCAC GGAATGCGGC 
AAGGGATTCT GCCAATCGAG AACCTTGGCT GTCCACAAGA TCCTGCACAT GGAGGAATCA 
CCCCACAAGT GCCCCGTCTG CAGTCGATCA TTCAATCAGC GCTCCAACCT GAAGACCCAT 
CTGCTCACCC ACACGGATCA CAAGCCCTAC GAGTGCTCTT CATGCGGCAA AGTTTTCCGC 
CGTAACTGCG ATCTACGACG CCATGCCTTG ACCCATGCAG TGGGTGAGGT CAACTCCGGG 
GACTATGTGG ATGTGGGCGA AGAGGATGAG GCCAGAAATT TAAGTGGCGA CGAGGAGGAT 
TCGTTGCTGG AAGTGGACTC GCCTCGCCAG TCGCCAGTTC ACAATTTGGG CGAGTCTGGA 
GGATCGGGTG AGAAATCTGA GTCCGAAAGA ATGAGACTCA AGCGCAAGGC AGCCATCGAT 
CATGAGGAAA GCGAAGAGGA GTTCGATGAC TTCGACGAGG AAGAGGAACT GCAGGATCTG 
CCAAGAGTAC ACGATTTGCC CAGGGAAGAG GACGACGACT TTGATCCCGA GGACGAGGAG 
CAGGCAGAAG TGGCACTAGT GGCGCGTTTC CAGGCGAGTA AGGCTGCCGC CACATCGCAA 
TCATCGTCGT CAGTGGGCAC CAAGCCGGAA CGCCAGGGAG TCACCCACTG TCATCATGAG 
GGTGGTGAAA CCTACACAAT GCGGCCACAT GGCGAGAAAC ATCAGGAGGA ACCCGGGAAC 
TCTGGCATTG CCAGCCTACC CGTTCCACCT TCCTTTGTGC GATATTCTGT ACCACCTGGG 
GCAGCTGGAC CGCCGCCTGC ACCACCTGGA GCTCCTCCGC CAACCCACCA GCACCCAGGA 
CATCCACACT TACTCCCACC CAACGGGGAT CCCTATTTGC CCATTCTGCA CGTTCGACGC 
GACTTGCACC ACAAGAGCTT GAACCTTTCG AAGGCTGGTG TTCCACCGCC ACCACATACA 
CCACCCACTA TAATAACCCA GCCGGAGTCG GGTAAACCAC CCAATCAACC TTTGCATTCA 
CCGCATGAAG CTATGCCCAG CTTCCTGGGA TCGATACCCA TGCGAAAGAG GATTCTTCCG 
GCACCGACCT TGGACTTGAT GGATCCGCAC CACCATCCTG GATTGGGTCA AAGGACCTTT 
GTGGATAGTC CCAGCATATA TGCTTTGAAT ATGTCGCGAC ATCCCCCCAG ACAGCTTCTG 
GGAAAACCAC CGAGTACGGA AACAAGTGGA GCCACCACCG AAAAGGGACC ACCAGTGGCA 
GCACCGCCCA TAGCACCACC GCCTGCACCA CCGAGAAGAA CAGGGTTCAG CATTGAGGAC 
ATAATGAGGC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245863.1
CDS	364..2598
Translation	MPTESSSSEISGGGGGAIPMLRPSRMDQFMNSMAAAAAAVGGGGLPGAADRNGGSGGSDGGSQNGNGDSRNSSASRISAYETQLAYQQHLAGLHGPPPPPPPSHHREISAFVPVLPTGKVRPGSNSNYEIIAMMADKRKELALREAAAAAAMLGRGPGGPGGPGVPPPGVLYGPAGVPPPPYLTGPGPSPTGAGSFPFPPGAAAAALFPPGLGPGMHAGLDRRLLRAPGRASRPKKQFICKFCNRQFTKSYNLLIHERTHTDERPYSCDICGKAFRRQDHLRDHRYIHSKEKPFKCTECGKGFCQSRTLAVHKILHMEESPHKCPVCSRSFNQRSNLKTHLLTHTDHKPYECSSCGKVFRRNCDLRRHALTHAVGEVNSGDYVDVGEEDEARNLSGDEEDSLLEVDSPRQSPVHNLGESGGSGEKSESERMRLKRKAAIDHEESEEEFDDFDEEEELQDLPRVHDLPREEDDDFDPEDEEQAEVALVARFQASKAAATSQSSSSVGTKPERQGVTHCHHEGGETYTMRPHGEKHQEEPGNSGIASLPVPPSFVRYSVPPGAAGPPPAPPGAPPPTHQHPGHPHLLPPNGDPYLPILHVRRDLHHKSLNLSKAGVPPPPHTPPTIITQPESGKPPNQPLHSPHEAMPSFLGSIPMRKRILPAPTLDLMDPHHHPGLGQRTFVDSPSIYALNMSRHPPRQLLGKPPSTETSGATTEKGPPVAAPPIAPPPAPPRRTGFSIEDIMRR

Target ORF information:

RefSeq Version	NM_001258934.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258934.1

ORF Insert Sequence:

ATGCCCACGG AAAGCTCATC CAGCGAGATC TCCGGCGGAG GAGGCGGTGC GATACCCATG 
CTGCGACCTT CCCGGATGGA TCAGTTCATG AACTCGATGG CGGCGGCAGC GGCGGCGGTG 
GGTGGTGGCG GTCTTCCTGG TGCGGCGGAT AGGAACGGCG GATCCGGAGG ATCGGATGGT 
GGCTCCCAGA ATGGCAACGG CGATTCGCGC AACTCATCGG CCAGCCGGAT CTCAGCGTAT 
GAAACACAGC TGGCCTATCA GCAGCATTTG GCCGGACTGC ATGGTCCGCC GCCACCGCCA 
CCACCATCGC ATCATCGCGA GATCTCTGCC TTTGTGCCGG TTCTGCCCAC CGGAAAGGTG 
CGCCCGGGTA GTAATTCCAA TTACGAAATC ATTGCCATGA TGGCGGACAA GCGAAAGGAG 
TTGGCCCTAA GGGAGGCGGC TGCGGCGGCG GCAATGTTAG GACGAGGTCC TGGAGGACCC 
GGCGGTCCTG GTGTGCCACC GCCTGGAGTG TTGTACGGAC CAGCTGGCGT GCCACCTCCG 
CCATATCTCA CCGGACCGGG TCCATCGCCC ACGGGAGCCG GAAGTTTTCC ATTTCCACCA 
GGAGCCGCCG CCGCAGCTCT ATTTCCACCT GGCCTGGGTC CTGGAATGCA TGCCGGACTC 
GATAGGCGCC TGCTACGTGC TCCTGGTCGG GCATCGCGGC CTAAAAAACA GTTCATCTGC 
AAGTTCTGCA ATCGACAATT CACCAAGTCG TACAATCTGC TCATCCACGA GAGGACACAC 
ACGGACGAGC GGCCCTATTC CTGTGATATC TGCGGCAAGG CGTTCCGGCG ACAGGATCAC 
CTGAGGGATC ACAGGTATAT TCACTCCAAG GAGAAACCCT TCAAATGCAC GGAATGCGGC 
AAGGGATTCT GCCAATCGAG AACCTTGGCT GTCCACAAGA TCCTGCACAT GGAGGAATCA 
CCCCACAAGT GCCCCGTCTG CAGTCGATCA TTCAATCAGC GCTCCAACCT GAAGACCCAT 
CTGCTCACCC ACACGGATCA CAAGCCCTAC GAGTGCTCTT CATGCGGCAA AGTTTTCCGC 
CGTAACTGCG ATCTACGACG CCATGCCTTG ACCCATGCAG TGGGTGAGGT CAACTCCGGG 
GACTATGTGG ATGTGGGCGA AGAGGATGAG GCCAGAAATT TAAGTGGCGA CGAGGAGGAT 
TCGTTGCTGG AAGTGGACTC GCCTCGCCAG TCGCCAGTTC ACAATTTGGG CGAGTCTGGA 
GGATCGGGTG AGAAATCTGA GTCCGAAAGA ATGAGACTCA AGCGCAAGGC AGCCATCGAT 
CATGAGGAAA GCGAAGAGGA GTTCGATGAC TTCGACGAGG AAGAGGAACT GCAGGATCTG 
CCAAGAGTAC ACGATTTGCC CAGGGAAGAG GACGACGACT TTGATCCCGA GGACGAGGAG 
CAGGCAGAAG TGGCACTAGT GGCGCGTTTC CAGGCGAGTA AGGCTGCCGC CACATCGCAA 
TCATCGTCGT CAGTGGGCAC CAAGCCGGAA CGCCAGGGAG TCACCCACTG TCATCATGAG 
GGTGGTGAAA CCTACACAAT GCGGCCACAT GGCGAGAAAC ATCAGGAGGA ACCCGGGAAC 
TCTGGCATTG CCAGCCTACC CGTTCCACCT TCCTTTGTGC GATATTCTGT ACCACCTGGG 
GCAGCTGGAC CGCCGCCTGC ACCACCTGGA GCTCCTCCGC CAACCCACCA GCACCCAGGA 
CATCCACACT TACTCCCACC CAACGGGGAT CCCTATTTGC CCATTCTGCA CGTTCGACGC 
GACTTGCACC ACAAGAGCTT GAACCTTTCG AAGGCTGGTG TTCCACCGCC ACCACATACA 
CCACCCACTA TAATAACCCA GCCGGAGTCG GGTAAACCAC CCAATCAACC TTTGCATTCA 
CCGCATGAAG CTATGCCCAG CTTCCTGGGA TCGATACCCA TGCGAAAGAG GATTCTTCCG 
GCACCGACCT TGGACTTGAT GGATCCGCAC CACCATCCTG GATTGGGTCA AAGGACCTTT 
GTGGATAGTC CCAGCATATA TGCTTTGAAT ATGTCGCGAC ATCCCCCCAG ACAGCTTCTG 
GGAAAACCAC CGAGTACGGA AACAAGTGGA GCCACCACCG AAAAGGGACC ACCAGTGGCA 
GCACCGCCCA TAGCACCACC GCCTGCACCA CCGAGAAGAA CAGGGTTCAG CATTGAGGAC 
ATAATGAGGC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bowl ( NM_164557.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722940.1 Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13682
Clone ID Related Accession (Same CDS sequence)	NM_057535.4 , NM_164558.2 , NM_001258934.1 , NM_164557.3 , NM_001258933.2 , NM_164556.2 , NM_001258932.2
Accession Version	NM_164557.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brother of odd with entrails limited, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164557.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCACGG AAAGCTCATC CAGCGAGATC TCCGGCGGAG GAGGCGGTGC GATACCCATG 
CTGCGACCTT CCCGGATGGA TCAGTTCATG AACTCGATGG CGGCGGCAGC GGCGGCGGTG 
GGTGGTGGCG GTCTTCCTGG TGCGGCGGAT AGGAACGGCG GATCCGGAGG ATCGGATGGT 
GGCTCCCAGA ATGGCAACGG CGATTCGCGC AACTCATCGG CCAGCCGGAT CTCAGCGTAT 
GAAACACAGC TGGCCTATCA GCAGCATTTG GCCGGACTGC ATGGTCCGCC GCCACCGCCA 
CCACCATCGC ATCATCGCGA GATCTCTGCC TTTGTGCCGG TTCTGCCCAC CGGAAAGGTG 
CGCCCGGGTA GTAATTCCAA TTACGAAATC ATTGCCATGA TGGCGGACAA GCGAAAGGAG 
TTGGCCCTAA GGGAGGCGGC TGCGGCGGCG GCAATGTTAG GACGAGGTCC TGGAGGACCC 
GGCGGTCCTG GTGTGCCACC GCCTGGAGTG TTGTACGGAC CAGCTGGCGT GCCACCTCCG 
CCATATCTCA CCGGACCGGG TCCATCGCCC ACGGGAGCCG GAAGTTTTCC ATTTCCACCA 
GGAGCCGCCG CCGCAGCTCT ATTTCCACCT GGCCTGGGTC CTGGAATGCA TGCCGGACTC 
GATAGGCGCC TGCTACGTGC TCCTGGTCGG GCATCGCGGC CTAAAAAACA GTTCATCTGC 
AAGTTCTGCA ATCGACAATT CACCAAGTCG TACAATCTGC TCATCCACGA GAGGACACAC 
ACGGACGAGC GGCCCTATTC CTGTGATATC TGCGGCAAGG CGTTCCGGCG ACAGGATCAC 
CTGAGGGATC ACAGGTATAT TCACTCCAAG GAGAAACCCT TCAAATGCAC GGAATGCGGC 
AAGGGATTCT GCCAATCGAG AACCTTGGCT GTCCACAAGA TCCTGCACAT GGAGGAATCA 
CCCCACAAGT GCCCCGTCTG CAGTCGATCA TTCAATCAGC GCTCCAACCT GAAGACCCAT 
CTGCTCACCC ACACGGATCA CAAGCCCTAC GAGTGCTCTT CATGCGGCAA AGTTTTCCGC 
CGTAACTGCG ATCTACGACG CCATGCCTTG ACCCATGCAG TGGGTGAGGT CAACTCCGGG 
GACTATGTGG ATGTGGGCGA AGAGGATGAG GCCAGAAATT TAAGTGGCGA CGAGGAGGAT 
TCGTTGCTGG AAGTGGACTC GCCTCGCCAG TCGCCAGTTC ACAATTTGGG CGAGTCTGGA 
GGATCGGGTG AGAAATCTGA GTCCGAAAGA ATGAGACTCA AGCGCAAGGC AGCCATCGAT 
CATGAGGAAA GCGAAGAGGA GTTCGATGAC TTCGACGAGG AAGAGGAACT GCAGGATCTG 
CCAAGAGTAC ACGATTTGCC CAGGGAAGAG GACGACGACT TTGATCCCGA GGACGAGGAG 
CAGGCAGAAG TGGCACTAGT GGCGCGTTTC CAGGCGAGTA AGGCTGCCGC CACATCGCAA 
TCATCGTCGT CAGTGGGCAC CAAGCCGGAA CGCCAGGGAG TCACCCACTG TCATCATGAG 
GGTGGTGAAA CCTACACAAT GCGGCCACAT GGCGAGAAAC ATCAGGAGGA ACCCGGGAAC 
TCTGGCATTG CCAGCCTACC CGTTCCACCT TCCTTTGTGC GATATTCTGT ACCACCTGGG 
GCAGCTGGAC CGCCGCCTGC ACCACCTGGA GCTCCTCCGC CAACCCACCA GCACCCAGGA 
CATCCACACT TACTCCCACC CAACGGGGAT CCCTATTTGC CCATTCTGCA CGTTCGACGC 
GACTTGCACC ACAAGAGCTT GAACCTTTCG AAGGCTGGTG TTCCACCGCC ACCACATACA 
CCACCCACTA TAATAACCCA GCCGGAGTCG GGTAAACCAC CCAATCAACC TTTGCATTCA 
CCGCATGAAG CTATGCCCAG CTTCCTGGGA TCGATACCCA TGCGAAAGAG GATTCTTCCG 
GCACCGACCT TGGACTTGAT GGATCCGCAC CACCATCCTG GATTGGGTCA AAGGACCTTT 
GTGGATAGTC CCAGCATATA TGCTTTGAAT ATGTCGCGAC ATCCCCCCAG ACAGCTTCTG 
GGAAAACCAC CGAGTACGGA AACAAGTGGA GCCACCACCG AAAAGGGACC ACCAGTGGCA 
GCACCGCCCA TAGCACCACC GCCTGCACCA CCGAGAAGAA CAGGGTTCAG CATTGAGGAC 
ATAATGAGGC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722940.1
CDS	442..2676
Translation	MPTESSSSEISGGGGGAIPMLRPSRMDQFMNSMAAAAAAVGGGGLPGAADRNGGSGGSDGGSQNGNGDSRNSSASRISAYETQLAYQQHLAGLHGPPPPPPPSHHREISAFVPVLPTGKVRPGSNSNYEIIAMMADKRKELALREAAAAAAMLGRGPGGPGGPGVPPPGVLYGPAGVPPPPYLTGPGPSPTGAGSFPFPPGAAAAALFPPGLGPGMHAGLDRRLLRAPGRASRPKKQFICKFCNRQFTKSYNLLIHERTHTDERPYSCDICGKAFRRQDHLRDHRYIHSKEKPFKCTECGKGFCQSRTLAVHKILHMEESPHKCPVCSRSFNQRSNLKTHLLTHTDHKPYECSSCGKVFRRNCDLRRHALTHAVGEVNSGDYVDVGEEDEARNLSGDEEDSLLEVDSPRQSPVHNLGESGGSGEKSESERMRLKRKAAIDHEESEEEFDDFDEEEELQDLPRVHDLPREEDDDFDPEDEEQAEVALVARFQASKAAATSQSSSSVGTKPERQGVTHCHHEGGETYTMRPHGEKHQEEPGNSGIASLPVPPSFVRYSVPPGAAGPPPAPPGAPPPTHQHPGHPHLLPPNGDPYLPILHVRRDLHHKSLNLSKAGVPPPPHTPPTIITQPESGKPPNQPLHSPHEAMPSFLGSIPMRKRILPAPTLDLMDPHHHPGLGQRTFVDSPSIYALNMSRHPPRQLLGKPPSTETSGATTEKGPPVAAPPIAPPPAPPRRTGFSIEDIMRR

Target ORF information:

RefSeq Version	NM_164557.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164557.3

ORF Insert Sequence:

ATGCCCACGG AAAGCTCATC CAGCGAGATC TCCGGCGGAG GAGGCGGTGC GATACCCATG 
CTGCGACCTT CCCGGATGGA TCAGTTCATG AACTCGATGG CGGCGGCAGC GGCGGCGGTG 
GGTGGTGGCG GTCTTCCTGG TGCGGCGGAT AGGAACGGCG GATCCGGAGG ATCGGATGGT 
GGCTCCCAGA ATGGCAACGG CGATTCGCGC AACTCATCGG CCAGCCGGAT CTCAGCGTAT 
GAAACACAGC TGGCCTATCA GCAGCATTTG GCCGGACTGC ATGGTCCGCC GCCACCGCCA 
CCACCATCGC ATCATCGCGA GATCTCTGCC TTTGTGCCGG TTCTGCCCAC CGGAAAGGTG 
CGCCCGGGTA GTAATTCCAA TTACGAAATC ATTGCCATGA TGGCGGACAA GCGAAAGGAG 
TTGGCCCTAA GGGAGGCGGC TGCGGCGGCG GCAATGTTAG GACGAGGTCC TGGAGGACCC 
GGCGGTCCTG GTGTGCCACC GCCTGGAGTG TTGTACGGAC CAGCTGGCGT GCCACCTCCG 
CCATATCTCA CCGGACCGGG TCCATCGCCC ACGGGAGCCG GAAGTTTTCC ATTTCCACCA 
GGAGCCGCCG CCGCAGCTCT ATTTCCACCT GGCCTGGGTC CTGGAATGCA TGCCGGACTC 
GATAGGCGCC TGCTACGTGC TCCTGGTCGG GCATCGCGGC CTAAAAAACA GTTCATCTGC 
AAGTTCTGCA ATCGACAATT CACCAAGTCG TACAATCTGC TCATCCACGA GAGGACACAC 
ACGGACGAGC GGCCCTATTC CTGTGATATC TGCGGCAAGG CGTTCCGGCG ACAGGATCAC 
CTGAGGGATC ACAGGTATAT TCACTCCAAG GAGAAACCCT TCAAATGCAC GGAATGCGGC 
AAGGGATTCT GCCAATCGAG AACCTTGGCT GTCCACAAGA TCCTGCACAT GGAGGAATCA 
CCCCACAAGT GCCCCGTCTG CAGTCGATCA TTCAATCAGC GCTCCAACCT GAAGACCCAT 
CTGCTCACCC ACACGGATCA CAAGCCCTAC GAGTGCTCTT CATGCGGCAA AGTTTTCCGC 
CGTAACTGCG ATCTACGACG CCATGCCTTG ACCCATGCAG TGGGTGAGGT CAACTCCGGG 
GACTATGTGG ATGTGGGCGA AGAGGATGAG GCCAGAAATT TAAGTGGCGA CGAGGAGGAT 
TCGTTGCTGG AAGTGGACTC GCCTCGCCAG TCGCCAGTTC ACAATTTGGG CGAGTCTGGA 
GGATCGGGTG AGAAATCTGA GTCCGAAAGA ATGAGACTCA AGCGCAAGGC AGCCATCGAT 
CATGAGGAAA GCGAAGAGGA GTTCGATGAC TTCGACGAGG AAGAGGAACT GCAGGATCTG 
CCAAGAGTAC ACGATTTGCC CAGGGAAGAG GACGACGACT TTGATCCCGA GGACGAGGAG 
CAGGCAGAAG TGGCACTAGT GGCGCGTTTC CAGGCGAGTA AGGCTGCCGC CACATCGCAA 
TCATCGTCGT CAGTGGGCAC CAAGCCGGAA CGCCAGGGAG TCACCCACTG TCATCATGAG 
GGTGGTGAAA CCTACACAAT GCGGCCACAT GGCGAGAAAC ATCAGGAGGA ACCCGGGAAC 
TCTGGCATTG CCAGCCTACC CGTTCCACCT TCCTTTGTGC GATATTCTGT ACCACCTGGG 
GCAGCTGGAC CGCCGCCTGC ACCACCTGGA GCTCCTCCGC CAACCCACCA GCACCCAGGA 
CATCCACACT TACTCCCACC CAACGGGGAT CCCTATTTGC CCATTCTGCA CGTTCGACGC 
GACTTGCACC ACAAGAGCTT GAACCTTTCG AAGGCTGGTG TTCCACCGCC ACCACATACA 
CCACCCACTA TAATAACCCA GCCGGAGTCG GGTAAACCAC CCAATCAACC TTTGCATTCA 
CCGCATGAAG CTATGCCCAG CTTCCTGGGA TCGATACCCA TGCGAAAGAG GATTCTTCCG 
GCACCGACCT TGGACTTGAT GGATCCGCAC CACCATCCTG GATTGGGTCA AAGGACCTTT 
GTGGATAGTC CCAGCATATA TGCTTTGAAT ATGTCGCGAC ATCCCCCCAG ACAGCTTCTG 
GGAAAACCAC CGAGTACGGA AACAAGTGGA GCCACCACCG AAAAGGGACC ACCAGTGGCA 
GCACCGCCCA TAGCACCACC GCCTGCACCA CCGAGAAGAA CAGGGTTCAG CATTGAGGAC 
ATAATGAGGC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bowl ( NM_001258933.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245862.1 Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa13682
Clone ID Related Accession (Same CDS sequence)	NM_057535.4 , NM_164558.2 , NM_001258934.1 , NM_164557.3 , NM_001258933.2 , NM_164556.2 , NM_001258932.2
Accession Version	NM_001258933.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brother of odd with entrails limited, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258933.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCACGG AAAGCTCATC CAGCGAGATC TCCGGCGGAG GAGGCGGTGC GATACCCATG 
CTGCGACCTT CCCGGATGGA TCAGTTCATG AACTCGATGG CGGCGGCAGC GGCGGCGGTG 
GGTGGTGGCG GTCTTCCTGG TGCGGCGGAT AGGAACGGCG GATCCGGAGG ATCGGATGGT 
GGCTCCCAGA ATGGCAACGG CGATTCGCGC AACTCATCGG CCAGCCGGAT CTCAGCGTAT 
GAAACACAGC TGGCCTATCA GCAGCATTTG GCCGGACTGC ATGGTCCGCC GCCACCGCCA 
CCACCATCGC ATCATCGCGA GATCTCTGCC TTTGTGCCGG TTCTGCCCAC CGGAAAGGTG 
CGCCCGGGTA GTAATTCCAA TTACGAAATC ATTGCCATGA TGGCGGACAA GCGAAAGGAG 
TTGGCCCTAA GGGAGGCGGC TGCGGCGGCG GCAATGTTAG GACGAGGTCC TGGAGGACCC 
GGCGGTCCTG GTGTGCCACC GCCTGGAGTG TTGTACGGAC CAGCTGGCGT GCCACCTCCG 
CCATATCTCA CCGGACCGGG TCCATCGCCC ACGGGAGCCG GAAGTTTTCC ATTTCCACCA 
GGAGCCGCCG CCGCAGCTCT ATTTCCACCT GGCCTGGGTC CTGGAATGCA TGCCGGACTC 
GATAGGCGCC TGCTACGTGC TCCTGGTCGG GCATCGCGGC CTAAAAAACA GTTCATCTGC 
AAGTTCTGCA ATCGACAATT CACCAAGTCG TACAATCTGC TCATCCACGA GAGGACACAC 
ACGGACGAGC GGCCCTATTC CTGTGATATC TGCGGCAAGG CGTTCCGGCG ACAGGATCAC 
CTGAGGGATC ACAGGTATAT TCACTCCAAG GAGAAACCCT TCAAATGCAC GGAATGCGGC 
AAGGGATTCT GCCAATCGAG AACCTTGGCT GTCCACAAGA TCCTGCACAT GGAGGAATCA 
CCCCACAAGT GCCCCGTCTG CAGTCGATCA TTCAATCAGC GCTCCAACCT GAAGACCCAT 
CTGCTCACCC ACACGGATCA CAAGCCCTAC GAGTGCTCTT CATGCGGCAA AGTTTTCCGC 
CGTAACTGCG ATCTACGACG CCATGCCTTG ACCCATGCAG TGGGTGAGGT CAACTCCGGG 
GACTATGTGG ATGTGGGCGA AGAGGATGAG GCCAGAAATT TAAGTGGCGA CGAGGAGGAT 
TCGTTGCTGG AAGTGGACTC GCCTCGCCAG TCGCCAGTTC ACAATTTGGG CGAGTCTGGA 
GGATCGGGTG AGAAATCTGA GTCCGAAAGA ATGAGACTCA AGCGCAAGGC AGCCATCGAT 
CATGAGGAAA GCGAAGAGGA GTTCGATGAC TTCGACGAGG AAGAGGAACT GCAGGATCTG 
CCAAGAGTAC ACGATTTGCC CAGGGAAGAG GACGACGACT TTGATCCCGA GGACGAGGAG 
CAGGCAGAAG TGGCACTAGT GGCGCGTTTC CAGGCGAGTA AGGCTGCCGC CACATCGCAA 
TCATCGTCGT CAGTGGGCAC CAAGCCGGAA CGCCAGGGAG TCACCCACTG TCATCATGAG 
GGTGGTGAAA CCTACACAAT GCGGCCACAT GGCGAGAAAC ATCAGGAGGA ACCCGGGAAC 
TCTGGCATTG CCAGCCTACC CGTTCCACCT TCCTTTGTGC GATATTCTGT ACCACCTGGG 
GCAGCTGGAC CGCCGCCTGC ACCACCTGGA GCTCCTCCGC CAACCCACCA GCACCCAGGA 
CATCCACACT TACTCCCACC CAACGGGGAT CCCTATTTGC CCATTCTGCA CGTTCGACGC 
GACTTGCACC ACAAGAGCTT GAACCTTTCG AAGGCTGGTG TTCCACCGCC ACCACATACA 
CCACCCACTA TAATAACCCA GCCGGAGTCG GGTAAACCAC CCAATCAACC TTTGCATTCA 
CCGCATGAAG CTATGCCCAG CTTCCTGGGA TCGATACCCA TGCGAAAGAG GATTCTTCCG 
GCACCGACCT TGGACTTGAT GGATCCGCAC CACCATCCTG GATTGGGTCA AAGGACCTTT 
GTGGATAGTC CCAGCATATA TGCTTTGAAT ATGTCGCGAC ATCCCCCCAG ACAGCTTCTG 
GGAAAACCAC CGAGTACGGA AACAAGTGGA GCCACCACCG AAAAGGGACC ACCAGTGGCA 
GCACCGCCCA TAGCACCACC GCCTGCACCA CCGAGAAGAA CAGGGTTCAG CATTGAGGAC 
ATAATGAGGC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245862.1
CDS	323..2557
Translation	MPTESSSSEISGGGGGAIPMLRPSRMDQFMNSMAAAAAAVGGGGLPGAADRNGGSGGSDGGSQNGNGDSRNSSASRISAYETQLAYQQHLAGLHGPPPPPPPSHHREISAFVPVLPTGKVRPGSNSNYEIIAMMADKRKELALREAAAAAAMLGRGPGGPGGPGVPPPGVLYGPAGVPPPPYLTGPGPSPTGAGSFPFPPGAAAAALFPPGLGPGMHAGLDRRLLRAPGRASRPKKQFICKFCNRQFTKSYNLLIHERTHTDERPYSCDICGKAFRRQDHLRDHRYIHSKEKPFKCTECGKGFCQSRTLAVHKILHMEESPHKCPVCSRSFNQRSNLKTHLLTHTDHKPYECSSCGKVFRRNCDLRRHALTHAVGEVNSGDYVDVGEEDEARNLSGDEEDSLLEVDSPRQSPVHNLGESGGSGEKSESERMRLKRKAAIDHEESEEEFDDFDEEEELQDLPRVHDLPREEDDDFDPEDEEQAEVALVARFQASKAAATSQSSSSVGTKPERQGVTHCHHEGGETYTMRPHGEKHQEEPGNSGIASLPVPPSFVRYSVPPGAAGPPPAPPGAPPPTHQHPGHPHLLPPNGDPYLPILHVRRDLHHKSLNLSKAGVPPPPHTPPTIITQPESGKPPNQPLHSPHEAMPSFLGSIPMRKRILPAPTLDLMDPHHHPGLGQRTFVDSPSIYALNMSRHPPRQLLGKPPSTETSGATTEKGPPVAAPPIAPPPAPPRRTGFSIEDIMRR

Target ORF information:

RefSeq Version	NM_001258933.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258933.2

ORF Insert Sequence:

ATGCCCACGG AAAGCTCATC CAGCGAGATC TCCGGCGGAG GAGGCGGTGC GATACCCATG 
CTGCGACCTT CCCGGATGGA TCAGTTCATG AACTCGATGG CGGCGGCAGC GGCGGCGGTG 
GGTGGTGGCG GTCTTCCTGG TGCGGCGGAT AGGAACGGCG GATCCGGAGG ATCGGATGGT 
GGCTCCCAGA ATGGCAACGG CGATTCGCGC AACTCATCGG CCAGCCGGAT CTCAGCGTAT 
GAAACACAGC TGGCCTATCA GCAGCATTTG GCCGGACTGC ATGGTCCGCC GCCACCGCCA 
CCACCATCGC ATCATCGCGA GATCTCTGCC TTTGTGCCGG TTCTGCCCAC CGGAAAGGTG 
CGCCCGGGTA GTAATTCCAA TTACGAAATC ATTGCCATGA TGGCGGACAA GCGAAAGGAG 
TTGGCCCTAA GGGAGGCGGC TGCGGCGGCG GCAATGTTAG GACGAGGTCC TGGAGGACCC 
GGCGGTCCTG GTGTGCCACC GCCTGGAGTG TTGTACGGAC CAGCTGGCGT GCCACCTCCG 
CCATATCTCA CCGGACCGGG TCCATCGCCC ACGGGAGCCG GAAGTTTTCC ATTTCCACCA 
GGAGCCGCCG CCGCAGCTCT ATTTCCACCT GGCCTGGGTC CTGGAATGCA TGCCGGACTC 
GATAGGCGCC TGCTACGTGC TCCTGGTCGG GCATCGCGGC CTAAAAAACA GTTCATCTGC 
AAGTTCTGCA ATCGACAATT CACCAAGTCG TACAATCTGC TCATCCACGA GAGGACACAC 
ACGGACGAGC GGCCCTATTC CTGTGATATC TGCGGCAAGG CGTTCCGGCG ACAGGATCAC 
CTGAGGGATC ACAGGTATAT TCACTCCAAG GAGAAACCCT TCAAATGCAC GGAATGCGGC 
AAGGGATTCT GCCAATCGAG AACCTTGGCT GTCCACAAGA TCCTGCACAT GGAGGAATCA 
CCCCACAAGT GCCCCGTCTG CAGTCGATCA TTCAATCAGC GCTCCAACCT GAAGACCCAT 
CTGCTCACCC ACACGGATCA CAAGCCCTAC GAGTGCTCTT CATGCGGCAA AGTTTTCCGC 
CGTAACTGCG ATCTACGACG CCATGCCTTG ACCCATGCAG TGGGTGAGGT CAACTCCGGG 
GACTATGTGG ATGTGGGCGA AGAGGATGAG GCCAGAAATT TAAGTGGCGA CGAGGAGGAT 
TCGTTGCTGG AAGTGGACTC GCCTCGCCAG TCGCCAGTTC ACAATTTGGG CGAGTCTGGA 
GGATCGGGTG AGAAATCTGA GTCCGAAAGA ATGAGACTCA AGCGCAAGGC AGCCATCGAT 
CATGAGGAAA GCGAAGAGGA GTTCGATGAC TTCGACGAGG AAGAGGAACT GCAGGATCTG 
CCAAGAGTAC ACGATTTGCC CAGGGAAGAG GACGACGACT TTGATCCCGA GGACGAGGAG 
CAGGCAGAAG TGGCACTAGT GGCGCGTTTC CAGGCGAGTA AGGCTGCCGC CACATCGCAA 
TCATCGTCGT CAGTGGGCAC CAAGCCGGAA CGCCAGGGAG TCACCCACTG TCATCATGAG 
GGTGGTGAAA CCTACACAAT GCGGCCACAT GGCGAGAAAC ATCAGGAGGA ACCCGGGAAC 
TCTGGCATTG CCAGCCTACC CGTTCCACCT TCCTTTGTGC GATATTCTGT ACCACCTGGG 
GCAGCTGGAC CGCCGCCTGC ACCACCTGGA GCTCCTCCGC CAACCCACCA GCACCCAGGA 
CATCCACACT TACTCCCACC CAACGGGGAT CCCTATTTGC CCATTCTGCA CGTTCGACGC 
GACTTGCACC ACAAGAGCTT GAACCTTTCG AAGGCTGGTG TTCCACCGCC ACCACATACA 
CCACCCACTA TAATAACCCA GCCGGAGTCG GGTAAACCAC CCAATCAACC TTTGCATTCA 
CCGCATGAAG CTATGCCCAG CTTCCTGGGA TCGATACCCA TGCGAAAGAG GATTCTTCCG 
GCACCGACCT TGGACTTGAT GGATCCGCAC CACCATCCTG GATTGGGTCA AAGGACCTTT 
GTGGATAGTC CCAGCATATA TGCTTTGAAT ATGTCGCGAC ATCCCCCCAG ACAGCTTCTG 
GGAAAACCAC CGAGTACGGA AACAAGTGGA GCCACCACCG AAAAGGGACC ACCAGTGGCA 
GCACCGCCCA TAGCACCACC GCCTGCACCA CCGAGAAGAA CAGGGTTCAG CATTGAGGAC 
ATAATGAGGC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bowl ( NM_164556.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722939.1 Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant A, mRNA.

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CloneID	OFa13682
Clone ID Related Accession (Same CDS sequence)	NM_057535.4 , NM_164558.2 , NM_001258934.1 , NM_164557.3 , NM_001258933.2 , NM_164556.2 , NM_001258932.2
Accession Version	NM_164556.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brother of odd with entrails limited, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On May 8, 2012 this sequence version replaced NM_164556.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCACGG AAAGCTCATC CAGCGAGATC TCCGGCGGAG GAGGCGGTGC GATACCCATG 
CTGCGACCTT CCCGGATGGA TCAGTTCATG AACTCGATGG CGGCGGCAGC GGCGGCGGTG 
GGTGGTGGCG GTCTTCCTGG TGCGGCGGAT AGGAACGGCG GATCCGGAGG ATCGGATGGT 
GGCTCCCAGA ATGGCAACGG CGATTCGCGC AACTCATCGG CCAGCCGGAT CTCAGCGTAT 
GAAACACAGC TGGCCTATCA GCAGCATTTG GCCGGACTGC ATGGTCCGCC GCCACCGCCA 
CCACCATCGC ATCATCGCGA GATCTCTGCC TTTGTGCCGG TTCTGCCCAC CGGAAAGGTG 
CGCCCGGGTA GTAATTCCAA TTACGAAATC ATTGCCATGA TGGCGGACAA GCGAAAGGAG 
TTGGCCCTAA GGGAGGCGGC TGCGGCGGCG GCAATGTTAG GACGAGGTCC TGGAGGACCC 
GGCGGTCCTG GTGTGCCACC GCCTGGAGTG TTGTACGGAC CAGCTGGCGT GCCACCTCCG 
CCATATCTCA CCGGACCGGG TCCATCGCCC ACGGGAGCCG GAAGTTTTCC ATTTCCACCA 
GGAGCCGCCG CCGCAGCTCT ATTTCCACCT GGCCTGGGTC CTGGAATGCA TGCCGGACTC 
GATAGGCGCC TGCTACGTGC TCCTGGTCGG GCATCGCGGC CTAAAAAACA GTTCATCTGC 
AAGTTCTGCA ATCGACAATT CACCAAGTCG TACAATCTGC TCATCCACGA GAGGACACAC 
ACGGACGAGC GGCCCTATTC CTGTGATATC TGCGGCAAGG CGTTCCGGCG ACAGGATCAC 
CTGAGGGATC ACAGGTATAT TCACTCCAAG GAGAAACCCT TCAAATGCAC GGAATGCGGC 
AAGGGATTCT GCCAATCGAG AACCTTGGCT GTCCACAAGA TCCTGCACAT GGAGGAATCA 
CCCCACAAGT GCCCCGTCTG CAGTCGATCA TTCAATCAGC GCTCCAACCT GAAGACCCAT 
CTGCTCACCC ACACGGATCA CAAGCCCTAC GAGTGCTCTT CATGCGGCAA AGTTTTCCGC 
CGTAACTGCG ATCTACGACG CCATGCCTTG ACCCATGCAG TGGGTGAGGT CAACTCCGGG 
GACTATGTGG ATGTGGGCGA AGAGGATGAG GCCAGAAATT TAAGTGGCGA CGAGGAGGAT 
TCGTTGCTGG AAGTGGACTC GCCTCGCCAG TCGCCAGTTC ACAATTTGGG CGAGTCTGGA 
GGATCGGGTG AGAAATCTGA GTCCGAAAGA ATGAGACTCA AGCGCAAGGC AGCCATCGAT 
CATGAGGAAA GCGAAGAGGA GTTCGATGAC TTCGACGAGG AAGAGGAACT GCAGGATCTG 
CCAAGAGTAC ACGATTTGCC CAGGGAAGAG GACGACGACT TTGATCCCGA GGACGAGGAG 
CAGGCAGAAG TGGCACTAGT GGCGCGTTTC CAGGCGAGTA AGGCTGCCGC CACATCGCAA 
TCATCGTCGT CAGTGGGCAC CAAGCCGGAA CGCCAGGGAG TCACCCACTG TCATCATGAG 
GGTGGTGAAA CCTACACAAT GCGGCCACAT GGCGAGAAAC ATCAGGAGGA ACCCGGGAAC 
TCTGGCATTG CCAGCCTACC CGTTCCACCT TCCTTTGTGC GATATTCTGT ACCACCTGGG 
GCAGCTGGAC CGCCGCCTGC ACCACCTGGA GCTCCTCCGC CAACCCACCA GCACCCAGGA 
CATCCACACT TACTCCCACC CAACGGGGAT CCCTATTTGC CCATTCTGCA CGTTCGACGC 
GACTTGCACC ACAAGAGCTT GAACCTTTCG AAGGCTGGTG TTCCACCGCC ACCACATACA 
CCACCCACTA TAATAACCCA GCCGGAGTCG GGTAAACCAC CCAATCAACC TTTGCATTCA 
CCGCATGAAG CTATGCCCAG CTTCCTGGGA TCGATACCCA TGCGAAAGAG GATTCTTCCG 
GCACCGACCT TGGACTTGAT GGATCCGCAC CACCATCCTG GATTGGGTCA AAGGACCTTT 
GTGGATAGTC CCAGCATATA TGCTTTGAAT ATGTCGCGAC ATCCCCCCAG ACAGCTTCTG 
GGAAAACCAC CGAGTACGGA AACAAGTGGA GCCACCACCG AAAAGGGACC ACCAGTGGCA 
GCACCGCCCA TAGCACCACC GCCTGCACCA CCGAGAAGAA CAGGGTTCAG CATTGAGGAC 
ATAATGAGGC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722939.1
CDS	414..2648
Translation	MPTESSSSEISGGGGGAIPMLRPSRMDQFMNSMAAAAAAVGGGGLPGAADRNGGSGGSDGGSQNGNGDSRNSSASRISAYETQLAYQQHLAGLHGPPPPPPPSHHREISAFVPVLPTGKVRPGSNSNYEIIAMMADKRKELALREAAAAAAMLGRGPGGPGGPGVPPPGVLYGPAGVPPPPYLTGPGPSPTGAGSFPFPPGAAAAALFPPGLGPGMHAGLDRRLLRAPGRASRPKKQFICKFCNRQFTKSYNLLIHERTHTDERPYSCDICGKAFRRQDHLRDHRYIHSKEKPFKCTECGKGFCQSRTLAVHKILHMEESPHKCPVCSRSFNQRSNLKTHLLTHTDHKPYECSSCGKVFRRNCDLRRHALTHAVGEVNSGDYVDVGEEDEARNLSGDEEDSLLEVDSPRQSPVHNLGESGGSGEKSESERMRLKRKAAIDHEESEEEFDDFDEEEELQDLPRVHDLPREEDDDFDPEDEEQAEVALVARFQASKAAATSQSSSSVGTKPERQGVTHCHHEGGETYTMRPHGEKHQEEPGNSGIASLPVPPSFVRYSVPPGAAGPPPAPPGAPPPTHQHPGHPHLLPPNGDPYLPILHVRRDLHHKSLNLSKAGVPPPPHTPPTIITQPESGKPPNQPLHSPHEAMPSFLGSIPMRKRILPAPTLDLMDPHHHPGLGQRTFVDSPSIYALNMSRHPPRQLLGKPPSTETSGATTEKGPPVAAPPIAPPPAPPRRTGFSIEDIMRR

Target ORF information:

RefSeq Version	NM_164556.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164556.2

ORF Insert Sequence:

ATGCCCACGG AAAGCTCATC CAGCGAGATC TCCGGCGGAG GAGGCGGTGC GATACCCATG 
CTGCGACCTT CCCGGATGGA TCAGTTCATG AACTCGATGG CGGCGGCAGC GGCGGCGGTG 
GGTGGTGGCG GTCTTCCTGG TGCGGCGGAT AGGAACGGCG GATCCGGAGG ATCGGATGGT 
GGCTCCCAGA ATGGCAACGG CGATTCGCGC AACTCATCGG CCAGCCGGAT CTCAGCGTAT 
GAAACACAGC TGGCCTATCA GCAGCATTTG GCCGGACTGC ATGGTCCGCC GCCACCGCCA 
CCACCATCGC ATCATCGCGA GATCTCTGCC TTTGTGCCGG TTCTGCCCAC CGGAAAGGTG 
CGCCCGGGTA GTAATTCCAA TTACGAAATC ATTGCCATGA TGGCGGACAA GCGAAAGGAG 
TTGGCCCTAA GGGAGGCGGC TGCGGCGGCG GCAATGTTAG GACGAGGTCC TGGAGGACCC 
GGCGGTCCTG GTGTGCCACC GCCTGGAGTG TTGTACGGAC CAGCTGGCGT GCCACCTCCG 
CCATATCTCA CCGGACCGGG TCCATCGCCC ACGGGAGCCG GAAGTTTTCC ATTTCCACCA 
GGAGCCGCCG CCGCAGCTCT ATTTCCACCT GGCCTGGGTC CTGGAATGCA TGCCGGACTC 
GATAGGCGCC TGCTACGTGC TCCTGGTCGG GCATCGCGGC CTAAAAAACA GTTCATCTGC 
AAGTTCTGCA ATCGACAATT CACCAAGTCG TACAATCTGC TCATCCACGA GAGGACACAC 
ACGGACGAGC GGCCCTATTC CTGTGATATC TGCGGCAAGG CGTTCCGGCG ACAGGATCAC 
CTGAGGGATC ACAGGTATAT TCACTCCAAG GAGAAACCCT TCAAATGCAC GGAATGCGGC 
AAGGGATTCT GCCAATCGAG AACCTTGGCT GTCCACAAGA TCCTGCACAT GGAGGAATCA 
CCCCACAAGT GCCCCGTCTG CAGTCGATCA TTCAATCAGC GCTCCAACCT GAAGACCCAT 
CTGCTCACCC ACACGGATCA CAAGCCCTAC GAGTGCTCTT CATGCGGCAA AGTTTTCCGC 
CGTAACTGCG ATCTACGACG CCATGCCTTG ACCCATGCAG TGGGTGAGGT CAACTCCGGG 
GACTATGTGG ATGTGGGCGA AGAGGATGAG GCCAGAAATT TAAGTGGCGA CGAGGAGGAT 
TCGTTGCTGG AAGTGGACTC GCCTCGCCAG TCGCCAGTTC ACAATTTGGG CGAGTCTGGA 
GGATCGGGTG AGAAATCTGA GTCCGAAAGA ATGAGACTCA AGCGCAAGGC AGCCATCGAT 
CATGAGGAAA GCGAAGAGGA GTTCGATGAC TTCGACGAGG AAGAGGAACT GCAGGATCTG 
CCAAGAGTAC ACGATTTGCC CAGGGAAGAG GACGACGACT TTGATCCCGA GGACGAGGAG 
CAGGCAGAAG TGGCACTAGT GGCGCGTTTC CAGGCGAGTA AGGCTGCCGC CACATCGCAA 
TCATCGTCGT CAGTGGGCAC CAAGCCGGAA CGCCAGGGAG TCACCCACTG TCATCATGAG 
GGTGGTGAAA CCTACACAAT GCGGCCACAT GGCGAGAAAC ATCAGGAGGA ACCCGGGAAC 
TCTGGCATTG CCAGCCTACC CGTTCCACCT TCCTTTGTGC GATATTCTGT ACCACCTGGG 
GCAGCTGGAC CGCCGCCTGC ACCACCTGGA GCTCCTCCGC CAACCCACCA GCACCCAGGA 
CATCCACACT TACTCCCACC CAACGGGGAT CCCTATTTGC CCATTCTGCA CGTTCGACGC 
GACTTGCACC ACAAGAGCTT GAACCTTTCG AAGGCTGGTG TTCCACCGCC ACCACATACA 
CCACCCACTA TAATAACCCA GCCGGAGTCG GGTAAACCAC CCAATCAACC TTTGCATTCA 
CCGCATGAAG CTATGCCCAG CTTCCTGGGA TCGATACCCA TGCGAAAGAG GATTCTTCCG 
GCACCGACCT TGGACTTGAT GGATCCGCAC CACCATCCTG GATTGGGTCA AAGGACCTTT 
GTGGATAGTC CCAGCATATA TGCTTTGAAT ATGTCGCGAC ATCCCCCCAG ACAGCTTCTG 
GGAAAACCAC CGAGTACGGA AACAAGTGGA GCCACCACCG AAAAGGGACC ACCAGTGGCA 
GCACCGCCCA TAGCACCACC GCCTGCACCA CCGAGAAGAA CAGGGTTCAG CATTGAGGAC 
ATAATGAGGC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bowl ( NM_001258932.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245861.1 Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant F, mRNA.

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CloneID	OFa13682
Clone ID Related Accession (Same CDS sequence)	NM_057535.4 , NM_164558.2 , NM_001258934.1 , NM_164557.3 , NM_001258933.2 , NM_164556.2 , NM_001258932.2
Accession Version	NM_001258932.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brother of odd with entrails limited, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258932.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCACGG AAAGCTCATC CAGCGAGATC TCCGGCGGAG GAGGCGGTGC GATACCCATG 
CTGCGACCTT CCCGGATGGA TCAGTTCATG AACTCGATGG CGGCGGCAGC GGCGGCGGTG 
GGTGGTGGCG GTCTTCCTGG TGCGGCGGAT AGGAACGGCG GATCCGGAGG ATCGGATGGT 
GGCTCCCAGA ATGGCAACGG CGATTCGCGC AACTCATCGG CCAGCCGGAT CTCAGCGTAT 
GAAACACAGC TGGCCTATCA GCAGCATTTG GCCGGACTGC ATGGTCCGCC GCCACCGCCA 
CCACCATCGC ATCATCGCGA GATCTCTGCC TTTGTGCCGG TTCTGCCCAC CGGAAAGGTG 
CGCCCGGGTA GTAATTCCAA TTACGAAATC ATTGCCATGA TGGCGGACAA GCGAAAGGAG 
TTGGCCCTAA GGGAGGCGGC TGCGGCGGCG GCAATGTTAG GACGAGGTCC TGGAGGACCC 
GGCGGTCCTG GTGTGCCACC GCCTGGAGTG TTGTACGGAC CAGCTGGCGT GCCACCTCCG 
CCATATCTCA CCGGACCGGG TCCATCGCCC ACGGGAGCCG GAAGTTTTCC ATTTCCACCA 
GGAGCCGCCG CCGCAGCTCT ATTTCCACCT GGCCTGGGTC CTGGAATGCA TGCCGGACTC 
GATAGGCGCC TGCTACGTGC TCCTGGTCGG GCATCGCGGC CTAAAAAACA GTTCATCTGC 
AAGTTCTGCA ATCGACAATT CACCAAGTCG TACAATCTGC TCATCCACGA GAGGACACAC 
ACGGACGAGC GGCCCTATTC CTGTGATATC TGCGGCAAGG CGTTCCGGCG ACAGGATCAC 
CTGAGGGATC ACAGGTATAT TCACTCCAAG GAGAAACCCT TCAAATGCAC GGAATGCGGC 
AAGGGATTCT GCCAATCGAG AACCTTGGCT GTCCACAAGA TCCTGCACAT GGAGGAATCA 
CCCCACAAGT GCCCCGTCTG CAGTCGATCA TTCAATCAGC GCTCCAACCT GAAGACCCAT 
CTGCTCACCC ACACGGATCA CAAGCCCTAC GAGTGCTCTT CATGCGGCAA AGTTTTCCGC 
CGTAACTGCG ATCTACGACG CCATGCCTTG ACCCATGCAG TGGGTGAGGT CAACTCCGGG 
GACTATGTGG ATGTGGGCGA AGAGGATGAG GCCAGAAATT TAAGTGGCGA CGAGGAGGAT 
TCGTTGCTGG AAGTGGACTC GCCTCGCCAG TCGCCAGTTC ACAATTTGGG CGAGTCTGGA 
GGATCGGGTG AGAAATCTGA GTCCGAAAGA ATGAGACTCA AGCGCAAGGC AGCCATCGAT 
CATGAGGAAA GCGAAGAGGA GTTCGATGAC TTCGACGAGG AAGAGGAACT GCAGGATCTG 
CCAAGAGTAC ACGATTTGCC CAGGGAAGAG GACGACGACT TTGATCCCGA GGACGAGGAG 
CAGGCAGAAG TGGCACTAGT GGCGCGTTTC CAGGCGAGTA AGGCTGCCGC CACATCGCAA 
TCATCGTCGT CAGTGGGCAC CAAGCCGGAA CGCCAGGGAG TCACCCACTG TCATCATGAG 
GGTGGTGAAA CCTACACAAT GCGGCCACAT GGCGAGAAAC ATCAGGAGGA ACCCGGGAAC 
TCTGGCATTG CCAGCCTACC CGTTCCACCT TCCTTTGTGC GATATTCTGT ACCACCTGGG 
GCAGCTGGAC CGCCGCCTGC ACCACCTGGA GCTCCTCCGC CAACCCACCA GCACCCAGGA 
CATCCACACT TACTCCCACC CAACGGGGAT CCCTATTTGC CCATTCTGCA CGTTCGACGC 
GACTTGCACC ACAAGAGCTT GAACCTTTCG AAGGCTGGTG TTCCACCGCC ACCACATACA 
CCACCCACTA TAATAACCCA GCCGGAGTCG GGTAAACCAC CCAATCAACC TTTGCATTCA 
CCGCATGAAG CTATGCCCAG CTTCCTGGGA TCGATACCCA TGCGAAAGAG GATTCTTCCG 
GCACCGACCT TGGACTTGAT GGATCCGCAC CACCATCCTG GATTGGGTCA AAGGACCTTT 
GTGGATAGTC CCAGCATATA TGCTTTGAAT ATGTCGCGAC ATCCCCCCAG ACAGCTTCTG 
GGAAAACCAC CGAGTACGGA AACAAGTGGA GCCACCACCG AAAAGGGACC ACCAGTGGCA 
GCACCGCCCA TAGCACCACC GCCTGCACCA CCGAGAAGAA CAGGGTTCAG CATTGAGGAC 
ATAATGAGGC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245861.1
CDS	436..2670
Translation	MPTESSSSEISGGGGGAIPMLRPSRMDQFMNSMAAAAAAVGGGGLPGAADRNGGSGGSDGGSQNGNGDSRNSSASRISAYETQLAYQQHLAGLHGPPPPPPPSHHREISAFVPVLPTGKVRPGSNSNYEIIAMMADKRKELALREAAAAAAMLGRGPGGPGGPGVPPPGVLYGPAGVPPPPYLTGPGPSPTGAGSFPFPPGAAAAALFPPGLGPGMHAGLDRRLLRAPGRASRPKKQFICKFCNRQFTKSYNLLIHERTHTDERPYSCDICGKAFRRQDHLRDHRYIHSKEKPFKCTECGKGFCQSRTLAVHKILHMEESPHKCPVCSRSFNQRSNLKTHLLTHTDHKPYECSSCGKVFRRNCDLRRHALTHAVGEVNSGDYVDVGEEDEARNLSGDEEDSLLEVDSPRQSPVHNLGESGGSGEKSESERMRLKRKAAIDHEESEEEFDDFDEEEELQDLPRVHDLPREEDDDFDPEDEEQAEVALVARFQASKAAATSQSSSSVGTKPERQGVTHCHHEGGETYTMRPHGEKHQEEPGNSGIASLPVPPSFVRYSVPPGAAGPPPAPPGAPPPTHQHPGHPHLLPPNGDPYLPILHVRRDLHHKSLNLSKAGVPPPPHTPPTIITQPESGKPPNQPLHSPHEAMPSFLGSIPMRKRILPAPTLDLMDPHHHPGLGQRTFVDSPSIYALNMSRHPPRQLLGKPPSTETSGATTEKGPPVAAPPIAPPPAPPRRTGFSIEDIMRR

Target ORF information:

RefSeq Version	NM_001258932.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brother of odd with entrails limited (bowl), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258932.2

ORF Insert Sequence:

ATGCCCACGG AAAGCTCATC CAGCGAGATC TCCGGCGGAG GAGGCGGTGC GATACCCATG 
CTGCGACCTT CCCGGATGGA TCAGTTCATG AACTCGATGG CGGCGGCAGC GGCGGCGGTG 
GGTGGTGGCG GTCTTCCTGG TGCGGCGGAT AGGAACGGCG GATCCGGAGG ATCGGATGGT 
GGCTCCCAGA ATGGCAACGG CGATTCGCGC AACTCATCGG CCAGCCGGAT CTCAGCGTAT 
GAAACACAGC TGGCCTATCA GCAGCATTTG GCCGGACTGC ATGGTCCGCC GCCACCGCCA 
CCACCATCGC ATCATCGCGA GATCTCTGCC TTTGTGCCGG TTCTGCCCAC CGGAAAGGTG 
CGCCCGGGTA GTAATTCCAA TTACGAAATC ATTGCCATGA TGGCGGACAA GCGAAAGGAG 
TTGGCCCTAA GGGAGGCGGC TGCGGCGGCG GCAATGTTAG GACGAGGTCC TGGAGGACCC 
GGCGGTCCTG GTGTGCCACC GCCTGGAGTG TTGTACGGAC CAGCTGGCGT GCCACCTCCG 
CCATATCTCA CCGGACCGGG TCCATCGCCC ACGGGAGCCG GAAGTTTTCC ATTTCCACCA 
GGAGCCGCCG CCGCAGCTCT ATTTCCACCT GGCCTGGGTC CTGGAATGCA TGCCGGACTC 
GATAGGCGCC TGCTACGTGC TCCTGGTCGG GCATCGCGGC CTAAAAAACA GTTCATCTGC 
AAGTTCTGCA ATCGACAATT CACCAAGTCG TACAATCTGC TCATCCACGA GAGGACACAC 
ACGGACGAGC GGCCCTATTC CTGTGATATC TGCGGCAAGG CGTTCCGGCG ACAGGATCAC 
CTGAGGGATC ACAGGTATAT TCACTCCAAG GAGAAACCCT TCAAATGCAC GGAATGCGGC 
AAGGGATTCT GCCAATCGAG AACCTTGGCT GTCCACAAGA TCCTGCACAT GGAGGAATCA 
CCCCACAAGT GCCCCGTCTG CAGTCGATCA TTCAATCAGC GCTCCAACCT GAAGACCCAT 
CTGCTCACCC ACACGGATCA CAAGCCCTAC GAGTGCTCTT CATGCGGCAA AGTTTTCCGC 
CGTAACTGCG ATCTACGACG CCATGCCTTG ACCCATGCAG TGGGTGAGGT CAACTCCGGG 
GACTATGTGG ATGTGGGCGA AGAGGATGAG GCCAGAAATT TAAGTGGCGA CGAGGAGGAT 
TCGTTGCTGG AAGTGGACTC GCCTCGCCAG TCGCCAGTTC ACAATTTGGG CGAGTCTGGA 
GGATCGGGTG AGAAATCTGA GTCCGAAAGA ATGAGACTCA AGCGCAAGGC AGCCATCGAT 
CATGAGGAAA GCGAAGAGGA GTTCGATGAC TTCGACGAGG AAGAGGAACT GCAGGATCTG 
CCAAGAGTAC ACGATTTGCC CAGGGAAGAG GACGACGACT TTGATCCCGA GGACGAGGAG 
CAGGCAGAAG TGGCACTAGT GGCGCGTTTC CAGGCGAGTA AGGCTGCCGC CACATCGCAA 
TCATCGTCGT CAGTGGGCAC CAAGCCGGAA CGCCAGGGAG TCACCCACTG TCATCATGAG 
GGTGGTGAAA CCTACACAAT GCGGCCACAT GGCGAGAAAC ATCAGGAGGA ACCCGGGAAC 
TCTGGCATTG CCAGCCTACC CGTTCCACCT TCCTTTGTGC GATATTCTGT ACCACCTGGG 
GCAGCTGGAC CGCCGCCTGC ACCACCTGGA GCTCCTCCGC CAACCCACCA GCACCCAGGA 
CATCCACACT TACTCCCACC CAACGGGGAT CCCTATTTGC CCATTCTGCA CGTTCGACGC 
GACTTGCACC ACAAGAGCTT GAACCTTTCG AAGGCTGGTG TTCCACCGCC ACCACATACA 
CCACCCACTA TAATAACCCA GCCGGAGTCG GGTAAACCAC CCAATCAACC TTTGCATTCA 
CCGCATGAAG CTATGCCCAG CTTCCTGGGA TCGATACCCA TGCGAAAGAG GATTCTTCCG 
GCACCGACCT TGGACTTGAT GGATCCGCAC CACCATCCTG GATTGGGTCA AAGGACCTTT 
GTGGATAGTC CCAGCATATA TGCTTTGAAT ATGTCGCGAC ATCCCCCCAG ACAGCTTCTG 
GGAAAACCAC CGAGTACGGA AACAAGTGGA GCCACCACCG AAAAGGGACC ACCAGTGGCA 
GCACCGCCCA TAGCACCACC GCCTGCACCA CCGAGAAGAA CAGGGTTCAG CATTGAGGAC 
ATAATGAGGC GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bowl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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