Spindly cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spindly
Entrez Gene ID	33578
Full Name	CG15415 gene product from transcript CG15415-RB
Synonyms	CG15415,Dmel\CG15415
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 24A1-24A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_134947.2	NP_608791.2	spindly

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spindly is a novel regulator of mitotic dynein, functioning specifically to target dynein to kinetochores.
Title: Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore.

The following Spindly gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spindly cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13663	NM_134947.2 Latest version!	Drosophila melanogaster spindly (Spindly), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spindly ( NM_134947.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608791.2 Drosophila melanogaster spindly (Spindly), mRNA.

Price & Availability↑

CloneID	OFa13663
Clone ID Related Accession (Same CDS sequence)	NM_134947.2
Accession Version	NM_134947.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spindly
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Dec 17, 2009 this sequence version replaced NM_134947.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACAAAA ACTTGGACCT TGACACCCTT AGCGTGGATG ACATCGTGGA GGAGTACAAG 
CTGCTGCACG ATCGCCACCA GCAGCTGAAA GACCAGAGCG AAAAGGATGC GCAAAGGATT 
TATGAGCTAA AAAGGAATCT GGACACTGCC CTCGCCGCCG AATCGTATTT GACCCAGGAA 
CTGGAGCATC TGAGTAGCCA GCCTGTGGCC ATCGCGTCTG GAGGGAATGG CCAGGAACTG 
GAGGAGCTGC GTCGAAAGTT CAAGACCCTG AAAGAGGAAC AGGATGTCCT ACAGCAAGAC 
TACGACGCCA AGGTGGAGGA GACCAGATCA CTGAAAGATA AGCTGACTGC CGCTGAAAAG 
AATCTGGCGG AAAAGTCCGC CTCCAGGTCA AATCAGCTTC ACGATGACTT TTTTGCCCGT 
TTGAATGCCC TAGAACTCGA AAACTGTGAG CTGTTGCAAA AGCTGGCCGA GTATGAGGAC 
TTGCGCATCG CAAACACTCT GGCGGTGGCT GAAAAAGAGA AAACCATCGA ATGTCTGCAA 
GATCGGGTGA GCTGCATGGA ACAGAATCTG AACTGCAAGC GGGATGAACT GGAGGAAAAG 
CTGCAATTGC TGGAGAGTTG CCAGGAGCAA CTGGTGGATG CCAATGCCAA GATAGCACTG 
CTTACCTCCG CCCCTGAAAA CAATGACCGC AAGGGCAACT CTCTGTTCGC TGAGGTGGAC 
GACCAAAGGC AAGCGATGAA GCAGATCCTG GCGGCCCAGA AAAAAAGCTA TCTGGATATG 
AAGAAGATCT TCACGGACAG CCAATTTGAG ATCAGACGTC TCAAGCGGGA AAACGTGGCC 
ATGCACACGG AGCTGCAGGC CTGTAGCACA ATCTTTTGCA GTGCGGACAA GACCTATCAA 
AATAAGCTGA ATGAGCGCAT CCGGCAACTG ATGACTGCCA ACGACAGCCT AGAGCGGCAG 
CTTAATCTCT CCCAGGAGCG GCTGCGCCAG CTGGCCAGCG AGAAGTCGGT CACCTGGCTG 
GACTCGATGC TGGATTTCTG CAAACGCGAA ACGGACGAGC TGAAGGCGCA GCTGCATAGC 
ATGCGAATCC AGAAGGCCGG ACTGGAGGAA CGGATGCGAT GCGTTCAGCA GGAAATGGCA 
CGCTGGCGCT TTGAGTCCCT AAAATCCCGC TGTGTGCTCA TTGATCGCGA AAATCTGCTG 
GCGGAGCATA AGATCGTTTT CAAGCCCATG CAGGCCATGG AGTTTAACAT CAAGGAGGCG 
GAACTTAAAA CTGCATTGCC CCGCATTGTC AGTGTAGCGG CACAATCTCC ATCATCATCA 
GCTCAACTAC ATACACATCC AGAAACATCT TTGAAATCGG CTGCGGAAAC CACAAGCAAG 
TCTTTTCCAA CTGCAACGGA AATTATTGTT CTTGATGCGC CCTTAAAGCC TGCCATTAAA 
GAAGAGGTAG TGGAGCCACT TAGCCAAGAA TTAAAACCAG CACTAAAAGA CACATCCGTA 
AAAGTCGAAC AGGAACTAGA GGTATCCGAA AGCAAGGCTT GTATAAAAAG AACACCTATT 
AAGACAATTA TGGGTATGAA AATAAAACGA GAAATCGACT TAAACTTGGA ACAGCAGGAC 
GCGGTTGATA TCAAAAAGGA GTTGGAAGCT CCAGAATTAA TTCCAAAACC GCAGCTGAAA 
GGCACTCCAG TCAAAGCCAA AAACGATGGA GTAACACCTC AAATGAATAT ACAAGAAGAT 
GAAAACGAAG AAACGGGTCT AAATTCCGTG GAAACCCCAG CCAAACCTTT GATGAAGGGT 
ACCCCAGTTA AGCAGCGACT GGGAGGGATA AAGGTGAAGT CCAACGAAGA ACTGCTGGGA 
GCAAGACTGC CTGGTACAGT AACGCCTGCT AAACCGCAGC GCAAAGGAAC TCCTGTGAAG 
CTCATAAAGC TGGAAACGGA AACTGAAGAC AGCAGCGATG AGGCGGCCAA GGAATCTCCC 
ATCAAGCAAG ATGGGACATT GAGTGTGGAA ATTCTGGACA GTCCAACGCC ACAAAAACCG 
CAGCGCAAAG GAACTCCTGT GAGGATTCCC GTCATCAAAG ACTCGACCAA CGAAAATATC 
CGCTCTATCC TCAGCAAAAA ACGGCAAGTC TTCGCCGAAG ATTCGCAAAA GGCTGTGAAG 
TTTAGCGCGA ACGATCCCAT TGTTCACAAT TTGAGTCCGC TTGAGAGATG TCCAAATGGG 
GAAACCACCA AGGAAAATGA CAAGCCAAAG GAAAGTGAAG ACAACCAGGA CGTAAAACCC 
ACAATTAAGA AGCCAAACAT CATACGGCGT ATTGTTGTGA GCTCTCGTAA ACCAATTATG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608791.2
CDS	32..2377
Translation	MYKNLDLDTLSVDDIVEEYKLLHDRHQQLKDQSEKDAQRIYELKRNLDTALAAESYLTQELEHLSSQPVAIASGGNGQELEELRRKFKTLKEEQDVLQQDYDAKVEETRSLKDKLTAAEKNLAEKSASRSNQLHDDFFARLNALELENCELLQKLAEYEDLRIANTLAVAEKEKTIECLQDRVSCMEQNLNCKRDELEEKLQLLESCQEQLVDANAKIALLTSAPENNDRKGNSLFAEVDDQRQAMKQILAAQKKSYLDMKKIFTDSQFEIRRLKRENVAMHTELQACSTIFCSADKTYQNKLNERIRQLMTANDSLERQLNLSQERLRQLASEKSVTWLDSMLDFCKRETDELKAQLHSMRIQKAGLEERMRCVQQEMARWRFESLKSRCVLIDRENLLAEHKIVFKPMQAMEFNIKEAELKTALPRIVSVAAQSPSSSAQLHTHPETSLKSAAETTSKSFPTATEIIVLDAPLKPAIKEEVVEPLSQELKPALKDTSVKVEQELEVSESKACIKRTPIKTIMGMKIKREIDLNLEQQDAVDIKKELEAPELIPKPQLKGTPVKAKNDGVTPQMNIQEDENEETGLNSVETPAKPLMKGTPVKQRLGGIKVKSNEELLGARLPGTVTPAKPQRKGTPVKLIKLETETEDSSDEAAKESPIKQDGTLSVEILDSPTPQKPQRKGTPVRIPVIKDSTNENIRSILSKKRQVFAEDSQKAVKFSANDPIVHNLSPLERCPNGETTKENDKPKESEDNQDVKPTIKKPNIIRRIVVSSRKPIMK

Target ORF information:

RefSeq Version	NM_134947.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spindly (Spindly), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134947.2

ORF Insert Sequence:

ATGTACAAAA ACTTGGACCT TGACACCCTT AGCGTGGATG ACATCGTGGA GGAGTACAAG 
CTGCTGCACG ATCGCCACCA GCAGCTGAAA GACCAGAGCG AAAAGGATGC GCAAAGGATT 
TATGAGCTAA AAAGGAATCT GGACACTGCC CTCGCCGCCG AATCGTATTT GACCCAGGAA 
CTGGAGCATC TGAGTAGCCA GCCTGTGGCC ATCGCGTCTG GAGGGAATGG CCAGGAACTG 
GAGGAGCTGC GTCGAAAGTT CAAGACCCTG AAAGAGGAAC AGGATGTCCT ACAGCAAGAC 
TACGACGCCA AGGTGGAGGA GACCAGATCA CTGAAAGATA AGCTGACTGC CGCTGAAAAG 
AATCTGGCGG AAAAGTCCGC CTCCAGGTCA AATCAGCTTC ACGATGACTT TTTTGCCCGT 
TTGAATGCCC TAGAACTCGA AAACTGTGAG CTGTTGCAAA AGCTGGCCGA GTATGAGGAC 
TTGCGCATCG CAAACACTCT GGCGGTGGCT GAAAAAGAGA AAACCATCGA ATGTCTGCAA 
GATCGGGTGA GCTGCATGGA ACAGAATCTG AACTGCAAGC GGGATGAACT GGAGGAAAAG 
CTGCAATTGC TGGAGAGTTG CCAGGAGCAA CTGGTGGATG CCAATGCCAA GATAGCACTG 
CTTACCTCCG CCCCTGAAAA CAATGACCGC AAGGGCAACT CTCTGTTCGC TGAGGTGGAC 
GACCAAAGGC AAGCGATGAA GCAGATCCTG GCGGCCCAGA AAAAAAGCTA TCTGGATATG 
AAGAAGATCT TCACGGACAG CCAATTTGAG ATCAGACGTC TCAAGCGGGA AAACGTGGCC 
ATGCACACGG AGCTGCAGGC CTGTAGCACA ATCTTTTGCA GTGCGGACAA GACCTATCAA 
AATAAGCTGA ATGAGCGCAT CCGGCAACTG ATGACTGCCA ACGACAGCCT AGAGCGGCAG 
CTTAATCTCT CCCAGGAGCG GCTGCGCCAG CTGGCCAGCG AGAAGTCGGT CACCTGGCTG 
GACTCGATGC TGGATTTCTG CAAACGCGAA ACGGACGAGC TGAAGGCGCA GCTGCATAGC 
ATGCGAATCC AGAAGGCCGG ACTGGAGGAA CGGATGCGAT GCGTTCAGCA GGAAATGGCA 
CGCTGGCGCT TTGAGTCCCT AAAATCCCGC TGTGTGCTCA TTGATCGCGA AAATCTGCTG 
GCGGAGCATA AGATCGTTTT CAAGCCCATG CAGGCCATGG AGTTTAACAT CAAGGAGGCG 
GAACTTAAAA CTGCATTGCC CCGCATTGTC AGTGTAGCGG CACAATCTCC ATCATCATCA 
GCTCAACTAC ATACACATCC AGAAACATCT TTGAAATCGG CTGCGGAAAC CACAAGCAAG 
TCTTTTCCAA CTGCAACGGA AATTATTGTT CTTGATGCGC CCTTAAAGCC TGCCATTAAA 
GAAGAGGTAG TGGAGCCACT TAGCCAAGAA TTAAAACCAG CACTAAAAGA CACATCCGTA 
AAAGTCGAAC AGGAACTAGA GGTATCCGAA AGCAAGGCTT GTATAAAAAG AACACCTATT 
AAGACAATTA TGGGTATGAA AATAAAACGA GAAATCGACT TAAACTTGGA ACAGCAGGAC 
GCGGTTGATA TCAAAAAGGA GTTGGAAGCT CCAGAATTAA TTCCAAAACC GCAGCTGAAA 
GGCACTCCAG TCAAAGCCAA AAACGATGGA GTAACACCTC AAATGAATAT ACAAGAAGAT 
GAAAACGAAG AAACGGGTCT AAATTCCGTG GAAACCCCAG CCAAACCTTT GATGAAGGGT 
ACCCCAGTTA AGCAGCGACT GGGAGGGATA AAGGTGAAGT CCAACGAAGA ACTGCTGGGA 
GCAAGACTGC CTGGTACAGT AACGCCTGCT AAACCGCAGC GCAAAGGAAC TCCTGTGAAG 
CTCATAAAGC TGGAAACGGA AACTGAAGAC AGCAGCGATG AGGCGGCCAA GGAATCTCCC 
ATCAAGCAAG ATGGGACATT GAGTGTGGAA ATTCTGGACA GTCCAACGCC ACAAAAACCG 
CAGCGCAAAG GAACTCCTGT GAGGATTCCC GTCATCAAAG ACTCGACCAA CGAAAATATC 
CGCTCTATCC TCAGCAAAAA ACGGCAAGTC TTCGCCGAAG ATTCGCAAAA GGCTGTGAAG 
TTTAGCGCGA ACGATCCCAT TGTTCACAAT TTGAGTCCGC TTGAGAGATG TCCAAATGGG 
GAAACCACCA AGGAAAATGA CAAGCCAAAG GAAAGTGAAG ACAACCAGGA CGTAAAACCC 
ACAATTAAGA AGCCAAACAT CATACGGCGT ATTGTTGTGA GCTCTCGTAA ACCAATTATG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Spindly cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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