ORF » Species Summary » Drosophila melanogaster » tim cDNA ORF clone

tim cDNA ORF clone, Drosophila melanogaster(fruit fly)

  • Gene

  • Clones

  • gRNAs

The following tim gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tim cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa13655 NM_001144309.4
Latest version!		 Drosophila melanogaster timeless (tim), transcript variant O, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa16162 NM_001201757.2
Latest version!		 Drosophila melanogaster timeless (tim), transcript variant N, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa41339 NM_001347756.1
Latest version!		 Drosophila melanogaster timeless (tim), transcript variant T, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OFa13652 NM_001038783.3
Latest version!		 Drosophila melanogaster timeless (tim), transcript variant M, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa13653 NM_001014463.4
Latest version!		 Drosophila melanogaster timeless (tim), transcript variant S, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa13650 NM_001144308.3
Latest version!		 Drosophila melanogaster timeless (tim), transcript variant R, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa13651 NM_164542.5
Latest version!		 Drosophila melanogaster timeless (tim), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa13651 NM_164540.4
Latest version!		 Drosophila melanogaster timeless (tim), transcript variant P, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa13654 NM_164541.6
Latest version!		 Drosophila melanogaster timeless (tim), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    tim ( NM_001144309.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137781.2 Drosophila melanogaster timeless (tim), transcript variant O, mRNA.
    Price & Availability↑
    CloneID OFa13655
    Clone ID Related Accession (Same CDS sequence) NM_001144309.4
    Accession Version NM_001144309.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product timeless, isoform O
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001144309.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
    CAAAAACTAC TTAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
    AGTAATACCT CGCCCCCCAA ACAGGGCAGT GGCGATTCCA GCCCCATGCT GACCTCTGAT 
    CCTACCTCTG ATTCCTCGGA CAATGGCAGC AATGGCCGTG GCATGGGCGG TGGCATGCGG 
    GAAGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAGG GTCAGGAGTA TCAGAACGCC 
    ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
    AACGACAGCG AGCAGCCTGG ATCGCCGGAG CAATCGCAGC CCGCCGGCGA GTCCATGGAC 
    GATGGAGATT ACGAGGACCA GAGACACAGG CAACTGAACG AGCATGGCGA GGAGGATGAA 
    GATGAGGACG AAGTGGAGGA GGAAGAGTAC CTACAATTGG GCCCAGCCTC GGAGCCCCTA 
    AACTTAACAC AACAACCAGC TGACAAGGTC AACAACACTA CCAACCCAAC GTCCAGTGCG 
    CCACAAGGCT GCCTGGGCAA TGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
    TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
    TCTGCGGTCA AATTGGGCCA AAAGTCCCCA CATGCCGGCC AGCTCCAGCT GACCAAGGGC 
    AAGTGTTGCC CACAGAAGCG GGAATGCCCC TCCTCGCAGT CGGAGCTATC GGATTGCGGT 
    TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGATGATGGG 
    CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
    CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTTAGAC GCAAAAAACT GGTCAAGCGC 
    AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTACAGC ACACCCCCAC CGATGATGAC 
    ATCTCCAATC TGCTGAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
    GTGGAGGAAC TGCACATGCA ACTGCTTTCC AATGCGAAGG TGCCCATTGA CACATCGCAC 
    TTCTTCTGGC TGGTCACCTA CTTCCTGAAG TTTGCCGCCC AACTGGAGCT GGATATGGAG 
    CACATCGACA CTATTCTCAC CTACGATGTT TTGAGCTACT TGACCTATGA GGGTGTGTCC 
    CTATGTGAGC AACTGGAACT GAATGCCCGA CAGGAGGGCA GTGACCTGAA GCCCTATCTA 
    AGGCGAATGC ACTTGGTGGT GACGGCCATC CGGGAGTTCC TCCAGGCCAT TGATACGTAC 
    AACAAAGTGA CTCATCTGAA CGAGGACGAC AAAGCCCATT TGAGGCAGCT TCAGCTGCAG 
    ATTAGCGAAA TGTCCGATCT GAGGTGCCTT TTTGTGCTTC TGCTGAGGCG TTTCAATCCC 
    AGCATTCATT CCAAGCAGTA TCTTCAGGAT CTGGTGGTTA CCAATCACAT CCTCCTACTC 
    ATTCTGGACA GTTCGGCCAA ACTTGGTGGA TGTCAAACCA TTCGCCTGTC GGAGCACATA 
    ACACAGTTTG CCACGCTGGA GGTGATGCAC TACTATGGCA TTCTGTTGGA GGACTTCAAC 
    AACAACGGAG AGTTTGTCAA TGACTGCATC TTCACCATGA TGCATCACAT CGGTGGCGAT 
    CTGGGCCAGA TTGGGGTTCT ATTTCAACCA ATTATTTTGA AAACCTATTC AAGAATTTGG 
    GAAGCGGACT ATGAACTGTG CGATGACTGG TCTGATCTTA TCGAGTATGT GATTCACAAG 
    TTCATGAATA CTCCTCCGAA GTCGCCACTC ACCATTCCTA CAACTTCCTT GACGGAAATG 
    ACCAAGGAAC ACAACCAGGA GCATACCGTT TGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137781.2
    CDS730..3405
    Translation

    Target ORF information:

    RefSeq Version NM_001144309.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster timeless (tim), transcript variant O, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001144309.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
    CAAAAACTAC TTAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
    AGTAATACCT CGCCCCCCAA ACAGGGCAGT GGCGATTCCA GCCCCATGCT GACCTCTGAT 
    CCTACCTCTG ATTCCTCGGA CAATGGCAGC AATGGCCGTG GCATGGGCGG TGGCATGCGG 
    GAAGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAGG GTCAGGAGTA TCAGAACGCC 
    ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
    AACGACAGCG AGCAGCCTGG ATCGCCGGAG CAATCGCAGC CCGCCGGCGA GTCCATGGAC 
    GATGGAGATT ACGAGGACCA GAGACACAGG CAACTGAACG AGCATGGCGA GGAGGATGAA 
    GATGAGGACG AAGTGGAGGA GGAAGAGTAC CTACAATTGG GCCCAGCCTC GGAGCCCCTA 
    AACTTAACAC AACAACCAGC TGACAAGGTC AACAACACTA CCAACCCAAC GTCCAGTGCG 
    CCACAAGGCT GCCTGGGCAA TGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
    TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
    TCTGCGGTCA AATTGGGCCA AAAGTCCCCA CATGCCGGCC AGCTCCAGCT GACCAAGGGC 
    AAGTGTTGCC CACAGAAGCG GGAATGCCCC TCCTCGCAGT CGGAGCTATC GGATTGCGGT 
    TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGATGATGGG 
    CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
    CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTTAGAC GCAAAAAACT GGTCAAGCGC 
    AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTACAGC ACACCCCCAC CGATGATGAC 
    ATCTCCAATC TGCTGAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
    GTGGAGGAAC TGCACATGCA ACTGCTTTCC AATGCGAAGG TGCCCATTGA CACATCGCAC 
    TTCTTCTGGC TGGTCACCTA CTTCCTGAAG TTTGCCGCCC AACTGGAGCT GGATATGGAG 
    CACATCGACA CTATTCTCAC CTACGATGTT TTGAGCTACT TGACCTATGA GGGTGTGTCC 
    CTATGTGAGC AACTGGAACT GAATGCCCGA CAGGAGGGCA GTGACCTGAA GCCCTATCTA 
    AGGCGAATGC ACTTGGTGGT GACGGCCATC CGGGAGTTCC TCCAGGCCAT TGATACGTAC 
    AACAAAGTGA CTCATCTGAA CGAGGACGAC AAAGCCCATT TGAGGCAGCT TCAGCTGCAG 
    ATTAGCGAAA TGTCCGATCT GAGGTGCCTT TTTGTGCTTC TGCTGAGGCG TTTCAATCCC 
    AGCATTCATT CCAAGCAGTA TCTTCAGGAT CTGGTGGTTA CCAATCACAT CCTCCTACTC 
    ATTCTGGACA GTTCGGCCAA ACTTGGTGGA TGTCAAACCA TTCGCCTGTC GGAGCACATA 
    ACACAGTTTG CCACGCTGGA GGTGATGCAC TACTATGGCA TTCTGTTGGA GGACTTCAAC 
    AACAACGGAG AGTTTGTCAA TGACTGCATC TTCACCATGA TGCATCACAT CGGTGGCGAT 
    CTGGGCCAGA TTGGGGTTCT ATTTCAACCA ATTATTTTGA AAACCTATTC AAGAATTTGG 
    GAAGCGGACT ATGAACTGTG CGATGACTGG TCTGATCTTA TCGAGTATGT GATTCACAAG 
    TTCATGAATA CTCCTCCGAA GTCGCCACTC ACCATTCCTA CAACTTCCTT GACGGAAATG 
    ACCAAGGAAC ACAACCAGGA GCATACCGTT TGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    tim ( NM_001201757.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188686.2 Drosophila melanogaster timeless (tim), transcript variant N, mRNA.
    Price & Availability↑
    CloneID OFa16162
    Clone ID Related Accession (Same CDS sequence) NM_001201757.2
    Accession Version NM_001201757.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product timeless, isoform N
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001201757.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
    CAAAAACTAC TTAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
    AGTAATACCT CGCCCCCCAA ACAGGGCAGT GGCGATTCCA GCCCCATGCT GACCTCTGAT 
    CCTACCTCTG ATTCCTCGGA CAATGGCAGC AATGGCCGTG GCATGGGCGG TGGCATGCGG 
    GAAGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAGG GTCAGGAGTA TCAGAACGCC 
    ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
    AACGACAGCG AGCAGCCTGG ATCGCCGGAG CAATCGCAGC CCGCCGGCGA GTCCATGGAC 
    GATGGAGATT ACGAGGACCA GAGACACAGG CAACTGAACG AGCATGGCGA GGAGGATGAA 
    GATGAGGACG AAGTGGAGGA GGAAGAGTAC CTACAATTGG GCCCAGCCTC GGAGCCCCTA 
    AACTTAACAC AACAACCAGC TGACAAGGTC AACAACACTA CCAACCCAAC GTCCAGTGCG 
    CCACAAGGCT GCCTGGGCAA TGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
    TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
    TCTGCGGTCA AATTGGGCCA AAAGTCCCCA CATGCCGGCC AGCTCCAGCT GACCAAGGGC 
    AAGTGTTGCC CACAGAAGCG GGAATGCCCC TCCTCGCAGT CGGAGCTATC GGATTGCGGT 
    TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGATGATGGG 
    CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
    CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTTAGAC GCAAAAAACT GGTCAAGCGC 
    AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTACAGC ACACCCCCAC CGATGATGAC 
    ATCTCCAATC TGCTGAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
    GTGGAGGAAC TGCACATGCA ACTGCTTTCC AATGCGGTGC CCATTGACAC ATCGCACTTC 
    TTCTGGCTGG TCACCTACTT CCTGAAGTTT GCCGCCCAAC TGGAGCTGGA TATGGAGCAC 
    ATCGACACTA TTCTCACCTA CGATGTTTTG AGCTACTTGA CCTATGAGGG TGTGTCCCTA 
    TGTGAGCAAC TGGAACTGAA TGCCCGACAG GAGGGCAGTG ACCTGAAGCC CTATCTAAGG 
    CGAATGCACT TGGTGGTGAC GGCCATCCGG GAGTTCCTCC AGGCCATTGA TACGTACAAC 
    AAAGTGACTC ATCTGAACGA GGACGACAAA GCCCATTTGA GGCAGCTTCA GCTGCAGATT 
    AGCGAAATGT CCGATCTGAG GTGCCTTTTT GTGCTTCTGC TGAGGCGTTT CAATCCCAGC 
    ATTCATTCCA AGCAGTATCT TCAGGATCTG GTGGTTACCA ATCACATCCT CCTACTCATT 
    CTGGACAGTT CGGCCAAACT TGGTGGATGT CAAACCATTC GCCTGTCGGA GCACATAACA 
    CAGTTTGCCA CGCTGGAGGT GATGCACTAC TATGGCATTC TGTTGGAGGA CTTCAACAAC 
    AACGGAGAGT TTGTCAATGA CTGCATCTTC ACCATGATGC ATCACATCGG TGGCGATCTG 
    GGCCAGATTG GGGTTCTATT TCAACCAATT ATTTTGAAAA CCTATTCAAG AATTTGGGAA 
    GCGGACTATG AACTGTGCGA TGACTGGTCT GATCTTATCG AGTATGTGAT TCACAAGTTC 
    ATGAATACTC CTCCGAAGTC GCCACTCACC ATTCCTACAA CTTCCTTGAC GGAAATGACC 
    AAGGAACACA ACCAGGAGCA TACCGTTTGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001188686.2
    CDS344..3016
    Translation

    Target ORF information:

    RefSeq Version NM_001201757.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster timeless (tim), transcript variant N, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001201757.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
    CAAAAACTAC TTAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
    AGTAATACCT CGCCCCCCAA ACAGGGCAGT GGCGATTCCA GCCCCATGCT GACCTCTGAT 
    CCTACCTCTG ATTCCTCGGA CAATGGCAGC AATGGCCGTG GCATGGGCGG TGGCATGCGG 
    GAAGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAGG GTCAGGAGTA TCAGAACGCC 
    ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
    AACGACAGCG AGCAGCCTGG ATCGCCGGAG CAATCGCAGC CCGCCGGCGA GTCCATGGAC 
    GATGGAGATT ACGAGGACCA GAGACACAGG CAACTGAACG AGCATGGCGA GGAGGATGAA 
    GATGAGGACG AAGTGGAGGA GGAAGAGTAC CTACAATTGG GCCCAGCCTC GGAGCCCCTA 
    AACTTAACAC AACAACCAGC TGACAAGGTC AACAACACTA CCAACCCAAC GTCCAGTGCG 
    CCACAAGGCT GCCTGGGCAA TGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
    TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
    TCTGCGGTCA AATTGGGCCA AAAGTCCCCA CATGCCGGCC AGCTCCAGCT GACCAAGGGC 
    AAGTGTTGCC CACAGAAGCG GGAATGCCCC TCCTCGCAGT CGGAGCTATC GGATTGCGGT 
    TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGATGATGGG 
    CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
    CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTTAGAC GCAAAAAACT GGTCAAGCGC 
    AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTACAGC ACACCCCCAC CGATGATGAC 
    ATCTCCAATC TGCTGAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
    GTGGAGGAAC TGCACATGCA ACTGCTTTCC AATGCGGTGC CCATTGACAC ATCGCACTTC 
    TTCTGGCTGG TCACCTACTT CCTGAAGTTT GCCGCCCAAC TGGAGCTGGA TATGGAGCAC 
    ATCGACACTA TTCTCACCTA CGATGTTTTG AGCTACTTGA CCTATGAGGG TGTGTCCCTA 
    TGTGAGCAAC TGGAACTGAA TGCCCGACAG GAGGGCAGTG ACCTGAAGCC CTATCTAAGG 
    CGAATGCACT TGGTGGTGAC GGCCATCCGG GAGTTCCTCC AGGCCATTGA TACGTACAAC 
    AAAGTGACTC ATCTGAACGA GGACGACAAA GCCCATTTGA GGCAGCTTCA GCTGCAGATT 
    AGCGAAATGT CCGATCTGAG GTGCCTTTTT GTGCTTCTGC TGAGGCGTTT CAATCCCAGC 
    ATTCATTCCA AGCAGTATCT TCAGGATCTG GTGGTTACCA ATCACATCCT CCTACTCATT 
    CTGGACAGTT CGGCCAAACT TGGTGGATGT CAAACCATTC GCCTGTCGGA GCACATAACA 
    CAGTTTGCCA CGCTGGAGGT GATGCACTAC TATGGCATTC TGTTGGAGGA CTTCAACAAC 
    AACGGAGAGT TTGTCAATGA CTGCATCTTC ACCATGATGC ATCACATCGG TGGCGATCTG 
    GGCCAGATTG GGGTTCTATT TCAACCAATT ATTTTGAAAA CCTATTCAAG AATTTGGGAA 
    GCGGACTATG AACTGTGCGA TGACTGGTCT GATCTTATCG AGTATGTGAT TCACAAGTTC 
    ATGAATACTC CTCCGAAGTC GCCACTCACC ATTCCTACAA CTTCCTTGAC GGAAATGACC 
    AAGGAACACA ACCAGGAGCA TACCGTTTGG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    tim ( NM_001347756.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001334730.1 Drosophila melanogaster timeless (tim), transcript variant T, mRNA.
    Price & Availability↑
    CloneID OFa41339
    Clone ID Related Accession (Same CDS sequence) NM_001347756.1
    Accession Version NM_001347756.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product timeless, isoform T
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGCAGAG TTAGGCAGCT CCACAATCAC ATCTGGAATA ATCAGAACTT TGATAAAGTG 
    AAATCGGTTA TGGACTGGTT ACTAGCAACT CCGCAGTTGT ACAGCGCGTT CTCCTCCTTG 
    GGTTGCTTGG AGGGCGATAC CTATGTGGTC AACCCGAATG CATTGGCCAT TCTGGAGGAG 
    ATCAACTACA AGCTCACCTA TGAGGACCAA ACACTGCGCA CCTTTCGACG GGCCATTGGA 
    TTTGGCCAGA ATGTGAGGTC AGACCTGATA CCGCTGCTGG AGAATGCCAA GGATGATGCG 
    GTCCTGGAGT CGGTCATCCG GATACTCGTC AATCTGACGG TGCCGGTGGA GTGCCTCTTC 
    TCCGTGGACG TGATGTACCG CACGGATGTG GGTCGCCACA CCATCTTCGA GCTGAACAAG 
    CTGCTGTACA CCAGCAAGGA AGCATTTACC GAGGCCAGGA GCACCAAGAG CGTGGTGGAG 
    TACATGAAAC ACATACTGGA GTCGGACCCT AAGCTGTCGC CGCACAAATG CGATCAAATC 
    AACAACTGTC TGCTGCTGCT GAGAAATATC CTGCACATTC CAGAGACGCA TGCCCATTGC 
    GTGATGCCCA TGATGCAGTC GATGCCGCAT GGCATCTCCA TGCAGAACAC GATCTTGTGG 
    AATCTCTTCA TCCAGAGCAT CGACAAGTTA CTCCTGTATC TGATGACCTG TCCGCAGAGA 
    GCCTTCTGGG GAGTGACCAT GGTGCAACTG ATTGCTTTGA TCTACAAGGA TCAGCATGTG 
    AGCACTCTGC AAAAACTACT TAGCCTCTGG TTCGAAGCCT CTCTGTCGGA GAGCTCTGAG 
    GATAATGAGA GTAATACCTC GCCCCCCAAA CAGGGCAGTG GCGATTCCAG CCCCATGCTG 
    ACCTCTGATC CTACCTCTGA TTCCTCGGAC AATGGCAGCA ATGGCCGTGG CATGGGCGGT 
    GGCATGCGGG AAGGAACAGC GGCCACTTTG CAGGAGGTGA GCCGCAAGGG TCAGGAGTAT 
    CAGAACGCCA TGGCCAGAGT GCCAGCGGAT AAGCCCGATG GCTCCGAAGA GGCCAGCGAT 
    ATGACGGGGA ACGACAGCGA GCAGCCTGGA TCGCCGGAGC AATCGCAGCC CGCCGGCGAG 
    TCCATGGACG ATGGAGATTA CGAGGACCAG AGACACAGGC AACTGAACGA GCATGGCGAG 
    GAGGATGAAG ATGAGGACGA AGTGGAGGAG GAAGAGTACC TACAATTGGG CCCAGCCTCG 
    GAGCCCCTAA ACTTAACACA ACAACCAGCT GACAAGGTCA ACAACACTAC CAACCCAACG 
    TCCAGTGCGC CACAAGGCTG CCTGGGCAAT GAGCCATTCA AGCCACCACC TCCTCTGCCA 
    GTCAGAGCCT CCACCTCGGC ACACGCTCAA ATGCAGAAGT TCAACGAATC GTCCTACGCG 
    TCCCACGTAT CTGCGGTCAA ATTGGGCCAA AAGTCCCCAC ATGCCGGCCA GCTCCAGCTG 
    ACCAAGGGCA AGTGTTGCCC ACAGAAGCGG GAATGCCCCT CCTCGCAGTC GGAGCTATCG 
    GATTGCGGTT ATGGCACCCA GGTGGAAAAT CAGGAATCCA TTTCCACCTC CAGCAACGAC 
    GATGATGGGC CGCAGGGCAA GCCGCAGCAC CAGAAGCCTC CGTGTAACAC GAAGCCACGG 
    AATAAACCAC GGACGATTAT GTCGCCAATG GACAAAAAGG AGCTTAGACG CAAAAAACTG 
    GTCAAGCGCA GCAAAAGCAG CCTCATCAAC ATGAAGGGTC TGGTACAGCA CACCCCCACC 
    GATGATGACA TCTCCAATCT GCTGAAGGAA TTCACCGTGG ATTTCCTCCT CAAGGGTTAC 
    AGCTATCTGG TGGAGGAACT GCACATGCAA CTGCTTTCCA ATGCGAAGGT GCCCATTGAC 
    ACATCGCACT TCTTCTGGCT GGTCACCTAC TTCCTGAAGT TTGCCGCCCA ACTGGAGCTG 
    GATATGGAGC ACATCGACAC TATTCTCACC TACGATGTTT TGAGCTACTT GACCTATGAG 
    GGTGTGTCCC TATGTGAGCA ACTGGAACTG AATGCCCGAC AGGAGGGCAG TGACCTGAAG 
    CCCTATCTAA GGCGAATGCA CTTGGTGGTG ACGGCCATCC GGGAGTTCCT CCAGGCCATT 
    GATACGTACA ACAAAGTGAC TCATCTGAAC GAGGACGACA AAGCCCATTT GAGGCAGCTT 
    CAGCTGCAGA TTAGCGAAAT GTCCGATCTG AGGTGCCTTT TTGTGCTTCT GCTGAGGCGT 
    TTCAATCCCA GCATTCATTC CAAGCAGTAT CTTCAGGATC TGGTGGTTAC CAATCACATC 
    CTCCTACTCA TTCTGGACAG TTCGGCCAAA CTTGGTGGAT GTCAAACCAT TCGCCTGTCG 
    GAGCACATAA CACAGTTTGC CACGCTGGAG GTGATGCACT ACTATGGCAT TCTGTTGGAG 
    GACTTCAACA ACAACGGAGA GTTTGTCAAT GACTGCATCT TCACCATGAT GCATCACATC 
    GGTGGCGATC TGGGCCAGAT TGGGGTTCTA TTTCAACCAA TTATTTTGAA AACCTATTCA 
    AGAATTTGGG AAGCGGACTA TGAACTGTGC GATGACTGGT CTGATCTTAT CGAGTATGTG 
    ATTCACAAGT TCATGAATAC TCCTCCGAAG TCGCCACTCA CCATTCCTAC AACTTCCTTG 
    ACGGAAATGA CCAAGGAACA CAACCAGGAG CATACCGTTT GCTCTTGGTC GCAGGAGGAA 
    ATGGACACAC TTTATTGGTA TTATGTGCAG AGCAAGAAGA ACAACGATAT TGTGGGAAAG 
    ATAGTTAAGC TCTTCAGCAA CAACGGCAAC AAGCTGAAAA CCAGGATTTC TATTATCCAA 
    CAACTTTTGC AACAAGACAT TATCACCCTG TTGGAATACG ATGACCTGAT GAAGTTCGAG 
    GATGCGGAGT ATCAGAGAAC TTTGCTGACA ACTCCCACTT CCGCAACAAC AGAGTCTGGA 
    ATTGAGATTA AGGAGTGCGC CTACGGCAAA CCCTCAGATG ATGTTCAGAT CCTGCTGGAC 
    CTGATCATTA AGGAAAACAA GGCGCAGCAT TTGTTATGGC TGCAAAGGAT CCTCATTGAG 
    TGCTGCTTCG TTAAACTGAC CCTGCGGAGT GGTCTCAAGG TTCCGGAAGG CGATCACATC 
    ATGGAGCCGG TGGCCTACCA CTGCATCTGC AAGCAGAAGT CCATTCCGGT GGTGCAGTGG 
    AACAACGAGC AATCCACTAC GATGCTGTAC CAGCCTTTTG TTCTCCTGCT CCACAAGCTG 
    GGCATTCAGC TGCCGGCGGA CGCGGGCTCG ATCTTCGCCA GAATTCCGGA CTACTGGACA 
    CCGGAGACAA TGTACGGACT CGCCAAAAAG CTGGGACCGC TGGACAAACT CAACCTCAAG 
    TTCGACGCCA GTGAACTGGA GGATGCGACG GCGTCGAGTC CGTCGCGTTA CCACCACACC 
    GGACCCCGCA ACTCGCTCAG CTCGGTAAGC AGCCTGGACG TGGATCTCGG CGATACCGAG 
    GAGCTGGCCC TTATACCCGA GGTGGATGCG GCCGTGGAGA AGGCACACGC CATGGCATCC 
    ACGCCATCGC CCAGCGAGAT TTTCGCGGTT CCCAAGACGA AGCACTGCAA CTCGATCATC 
    AGATACACAC CAGATCCCAC GCCTCCAGTG CCCAACTGGC TGCAGTTGGT CATGCGCAGC 
    AAATGCAATC ATCGCACAGG TCCGTCTGGT GATCCCAGCG ATTGCATTGG CTCCTCGTCG 
    ACAACCGTGG ACGATGAGGG ATTTGGCAAG TCCATCAGTG CAGCCACTTC GCAGGCGGCG 
    AGCACCTCCA TGAGCACGGT TAATCCCACA ACCACTTTGA GCCTGAACAT GCTAAACACC 
    TTCATGGGAA GCCACAACGA GAACAGCAGC AGTTCTGGTT GCGGGGGCAC CGTCTCCTCC 
    CTGTCCATGG TGGCTCTGAT GAGCACCGGC GCGGCAGGCG GAGGAGGTAA CACCTCCGGG 
    CTGGAAATGG ATGTGGACGC CTCCATGAAG TCCTCGTTCG AGCGGCTGGA GGTAAACGGA 
    TCGCACTTCT CGCGGGCCAA CAACTTGGAC CAGGAGTACA GTGCCATGGT GGCATCTGTG 
    TACGAAAAGG AGAAGGAATT AAACAGCGAC AATGTCTCTT TGGCCTCGGA CCTGACCAGA 
    ATGTATGTGA GCGATGAGGA CGATCGACTT GAGCGAACCG AGATCCGGGT GCCCCACTAT 
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334730.1
    CDS275..4540
    Translation

    Target ORF information:

    RefSeq Version NM_001347756.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster timeless (tim), transcript variant T, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001347756.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGCAGAG TTAGGCAGCT CCACAATCAC ATCTGGAATA ATCAGAACTT TGATAAAGTG 
    AAATCGGTTA TGGACTGGTT ACTAGCAACT CCGCAGTTGT ACAGCGCGTT CTCCTCCTTG 
    GGTTGCTTGG AGGGCGATAC CTATGTGGTC AACCCGAATG CATTGGCCAT TCTGGAGGAG 
    ATCAACTACA AGCTCACCTA TGAGGACCAA ACACTGCGCA CCTTTCGACG GGCCATTGGA 
    TTTGGCCAGA ATGTGAGGTC AGACCTGATA CCGCTGCTGG AGAATGCCAA GGATGATGCG 
    GTCCTGGAGT CGGTCATCCG GATACTCGTC AATCTGACGG TGCCGGTGGA GTGCCTCTTC 
    TCCGTGGACG TGATGTACCG CACGGATGTG GGTCGCCACA CCATCTTCGA GCTGAACAAG 
    CTGCTGTACA CCAGCAAGGA AGCATTTACC GAGGCCAGGA GCACCAAGAG CGTGGTGGAG 
    TACATGAAAC ACATACTGGA GTCGGACCCT AAGCTGTCGC CGCACAAATG CGATCAAATC 
    AACAACTGTC TGCTGCTGCT GAGAAATATC CTGCACATTC CAGAGACGCA TGCCCATTGC 
    GTGATGCCCA TGATGCAGTC GATGCCGCAT GGCATCTCCA TGCAGAACAC GATCTTGTGG 
    AATCTCTTCA TCCAGAGCAT CGACAAGTTA CTCCTGTATC TGATGACCTG TCCGCAGAGA 
    GCCTTCTGGG GAGTGACCAT GGTGCAACTG ATTGCTTTGA TCTACAAGGA TCAGCATGTG 
    AGCACTCTGC AAAAACTACT TAGCCTCTGG TTCGAAGCCT CTCTGTCGGA GAGCTCTGAG 
    GATAATGAGA GTAATACCTC GCCCCCCAAA CAGGGCAGTG GCGATTCCAG CCCCATGCTG 
    ACCTCTGATC CTACCTCTGA TTCCTCGGAC AATGGCAGCA ATGGCCGTGG CATGGGCGGT 
    GGCATGCGGG AAGGAACAGC GGCCACTTTG CAGGAGGTGA GCCGCAAGGG TCAGGAGTAT 
    CAGAACGCCA TGGCCAGAGT GCCAGCGGAT AAGCCCGATG GCTCCGAAGA GGCCAGCGAT 
    ATGACGGGGA ACGACAGCGA GCAGCCTGGA TCGCCGGAGC AATCGCAGCC CGCCGGCGAG 
    TCCATGGACG ATGGAGATTA CGAGGACCAG AGACACAGGC AACTGAACGA GCATGGCGAG 
    GAGGATGAAG ATGAGGACGA AGTGGAGGAG GAAGAGTACC TACAATTGGG CCCAGCCTCG 
    GAGCCCCTAA ACTTAACACA ACAACCAGCT GACAAGGTCA ACAACACTAC CAACCCAACG 
    TCCAGTGCGC CACAAGGCTG CCTGGGCAAT GAGCCATTCA AGCCACCACC TCCTCTGCCA 
    GTCAGAGCCT CCACCTCGGC ACACGCTCAA ATGCAGAAGT TCAACGAATC GTCCTACGCG 
    TCCCACGTAT CTGCGGTCAA ATTGGGCCAA AAGTCCCCAC ATGCCGGCCA GCTCCAGCTG 
    ACCAAGGGCA AGTGTTGCCC ACAGAAGCGG GAATGCCCCT CCTCGCAGTC GGAGCTATCG 
    GATTGCGGTT ATGGCACCCA GGTGGAAAAT CAGGAATCCA TTTCCACCTC CAGCAACGAC 
    GATGATGGGC CGCAGGGCAA GCCGCAGCAC CAGAAGCCTC CGTGTAACAC GAAGCCACGG 
    AATAAACCAC GGACGATTAT GTCGCCAATG GACAAAAAGG AGCTTAGACG CAAAAAACTG 
    GTCAAGCGCA GCAAAAGCAG CCTCATCAAC ATGAAGGGTC TGGTACAGCA CACCCCCACC 
    GATGATGACA TCTCCAATCT GCTGAAGGAA TTCACCGTGG ATTTCCTCCT CAAGGGTTAC 
    AGCTATCTGG TGGAGGAACT GCACATGCAA CTGCTTTCCA ATGCGAAGGT GCCCATTGAC 
    ACATCGCACT TCTTCTGGCT GGTCACCTAC TTCCTGAAGT TTGCCGCCCA ACTGGAGCTG 
    GATATGGAGC ACATCGACAC TATTCTCACC TACGATGTTT TGAGCTACTT GACCTATGAG 
    GGTGTGTCCC TATGTGAGCA ACTGGAACTG AATGCCCGAC AGGAGGGCAG TGACCTGAAG 
    CCCTATCTAA GGCGAATGCA CTTGGTGGTG ACGGCCATCC GGGAGTTCCT CCAGGCCATT 
    GATACGTACA ACAAAGTGAC TCATCTGAAC GAGGACGACA AAGCCCATTT GAGGCAGCTT 
    CAGCTGCAGA TTAGCGAAAT GTCCGATCTG AGGTGCCTTT TTGTGCTTCT GCTGAGGCGT 
    TTCAATCCCA GCATTCATTC CAAGCAGTAT CTTCAGGATC TGGTGGTTAC CAATCACATC 
    CTCCTACTCA TTCTGGACAG TTCGGCCAAA CTTGGTGGAT GTCAAACCAT TCGCCTGTCG 
    GAGCACATAA CACAGTTTGC CACGCTGGAG GTGATGCACT ACTATGGCAT TCTGTTGGAG 
    GACTTCAACA ACAACGGAGA GTTTGTCAAT GACTGCATCT TCACCATGAT GCATCACATC 
    GGTGGCGATC TGGGCCAGAT TGGGGTTCTA TTTCAACCAA TTATTTTGAA AACCTATTCA 
    AGAATTTGGG AAGCGGACTA TGAACTGTGC GATGACTGGT CTGATCTTAT CGAGTATGTG 
    ATTCACAAGT TCATGAATAC TCCTCCGAAG TCGCCACTCA CCATTCCTAC AACTTCCTTG 
    ACGGAAATGA CCAAGGAACA CAACCAGGAG CATACCGTTT GCTCTTGGTC GCAGGAGGAA 
    ATGGACACAC TTTATTGGTA TTATGTGCAG AGCAAGAAGA ACAACGATAT TGTGGGAAAG 
    ATAGTTAAGC TCTTCAGCAA CAACGGCAAC AAGCTGAAAA CCAGGATTTC TATTATCCAA 
    CAACTTTTGC AACAAGACAT TATCACCCTG TTGGAATACG ATGACCTGAT GAAGTTCGAG 
    GATGCGGAGT ATCAGAGAAC TTTGCTGACA ACTCCCACTT CCGCAACAAC AGAGTCTGGA 
    ATTGAGATTA AGGAGTGCGC CTACGGCAAA CCCTCAGATG ATGTTCAGAT CCTGCTGGAC 
    CTGATCATTA AGGAAAACAA GGCGCAGCAT TTGTTATGGC TGCAAAGGAT CCTCATTGAG 
    TGCTGCTTCG TTAAACTGAC CCTGCGGAGT GGTCTCAAGG TTCCGGAAGG CGATCACATC 
    ATGGAGCCGG TGGCCTACCA CTGCATCTGC AAGCAGAAGT CCATTCCGGT GGTGCAGTGG 
    AACAACGAGC AATCCACTAC GATGCTGTAC CAGCCTTTTG TTCTCCTGCT CCACAAGCTG 
    GGCATTCAGC TGCCGGCGGA CGCGGGCTCG ATCTTCGCCA GAATTCCGGA CTACTGGACA 
    CCGGAGACAA TGTACGGACT CGCCAAAAAG CTGGGACCGC TGGACAAACT CAACCTCAAG 
    TTCGACGCCA GTGAACTGGA GGATGCGACG GCGTCGAGTC CGTCGCGTTA CCACCACACC 
    GGACCCCGCA ACTCGCTCAG CTCGGTAAGC AGCCTGGACG TGGATCTCGG CGATACCGAG 
    GAGCTGGCCC TTATACCCGA GGTGGATGCG GCCGTGGAGA AGGCACACGC CATGGCATCC 
    ACGCCATCGC CCAGCGAGAT TTTCGCGGTT CCCAAGACGA AGCACTGCAA CTCGATCATC 
    AGATACACAC CAGATCCCAC GCCTCCAGTG CCCAACTGGC TGCAGTTGGT CATGCGCAGC 
    AAATGCAATC ATCGCACAGG TCCGTCTGGT GATCCCAGCG ATTGCATTGG CTCCTCGTCG 
    ACAACCGTGG ACGATGAGGG ATTTGGCAAG TCCATCAGTG CAGCCACTTC GCAGGCGGCG 
    AGCACCTCCA TGAGCACGGT TAATCCCACA ACCACTTTGA GCCTGAACAT GCTAAACACC 
    TTCATGGGAA GCCACAACGA GAACAGCAGC AGTTCTGGTT GCGGGGGCAC CGTCTCCTCC 
    CTGTCCATGG TGGCTCTGAT GAGCACCGGC GCGGCAGGCG GAGGAGGTAA CACCTCCGGG 
    CTGGAAATGG ATGTGGACGC CTCCATGAAG TCCTCGTTCG AGCGGCTGGA GGTAAACGGA 
    TCGCACTTCT CGCGGGCCAA CAACTTGGAC CAGGAGTACA GTGCCATGGT GGCATCTGTG 
    TACGAAAAGG AGAAGGAATT AAACAGCGAC AATGTCTCTT TGGCCTCGGA CCTGACCAGA 
    ATGTATGTGA GCGATGAGGA CGATCGACTT GAGCGAACCG AGATCCGGGT GCCCCACTAT 
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    tim ( NM_001038783.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001033872.2 Drosophila melanogaster timeless (tim), transcript variant M, mRNA.
    Price & Availability↑
    CloneID OFa13652
    Clone ID Related Accession (Same CDS sequence) NM_001038783.3
    Accession Version NM_001038783.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product timeless, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001038783.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
    CAAAAACTAC TTAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
    AGTAATACCT CGCCCCCCAA ACAGGGCAGT GGCGATTCCA GCCCCATGCT GACCTCTGAT 
    CCTACCTCTG ATTCCTCGGA CAATGGCAGC AATGGCCGTG GCATGGGCGG TGGCATGCGG 
    GAAGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAGG GTCAGGAGTA TCAGAACGCC 
    ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
    AACGACAGCG AGCAGCCTGG ATCGCCGGAG CAATCGCAGC CCGCCGGCGA GTCCATGGAC 
    GATGGAGATT ACGAGGACCA GAGACACAGG CAACTGAACG AGCATGGCGA GGAGGATGAA 
    GATGAGGACG AAGTGGAGGA GGAAGAGTAC CTACAATTGG GCCCAGCCTC GGAGCCCCTA 
    AACTTAACAC AACAACCAGC TGACAAGGTC AACAACACTA CCAACCCAAC GTCCAGTGCG 
    CCACAAGGCT GCCTGGGCAA TGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
    TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
    TCTGCGGTCA AATTGGGCCA AAAGTCCCCA CATGCCGGCC AGCTCCAGCT GACCAAGGGC 
    AAGTGTTGCC CACAGAAGCG GGAATGCCCC TCCTCGCAGT CGGAGCTATC GGATTGCGGT 
    TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGATGATGGG 
    CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
    CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTTAGAC GCAAAAAACT GGTCAAGCGC 
    AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTACAGC ACACCCCCAC CGATGATGAC 
    ATCTCCAATC TGCTGAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
    GTGGAGGAAC TGCACATGCA ACTGCTTTCC AATGCGAAGG TGCCCATTGA CACATCGCAC 
    TTCTTCTGGC TGGTCACCTA CTTCCTGAAG TTTGCCGCCC AACTGGAGCT GGATATGGAG 
    CACATCGACA CTATTCTCAC CTACGATGTT TTGAGCTACT TGACCTATGA GGGTGTGTCC 
    CTATGTGAGC AACTGGAACT GAATGCCCGA CAGGAGGGCA GTGACCTGAA GCCCTATCTA 
    AGGCGAATGC ACTTGGTGGT GACGGCCATC CGGGAGTTCC TCCAGGCCAT TGATACGTAC 
    AACAAAGTGA CTCATCTGAA CGAGGACGAC AAAGCCCATT TGAGGCAGCT TCAGCTGCAG 
    ATTAGCGAAA TGTCCGATCT GAGGTGCCTT TTTGTGCTTC TGCTGAGGCG TTTCAATCCC 
    AGCATTCATT CCAAGCAGTA TCTTCAGGAT CTGGTGGTTA CCAATCACAT CCTCCTACTC 
    ATTCTGGACA GTTCGGCCAA ACTTGGTGGA TGTCAAACCA TTCGCCTGTC GGAGCACATA 
    ACACAGTTTG CCACGCTGGA GGTGATGCAC TACTATGGCA TTCTGTTGGA GGACTTCAAC 
    AACAACGGAG AGTTTGTCAA TGACTGCATC TTCACCATGA TGCATCACAT CGGTGGCGAT 
    CTGGGCCAGA TTGGGGTTCT ATTTCAACCA ATTATTTTGA AAACCTATTC AAGAATTTGG 
    GAAGCGGACT ATGAACTGTG CGATGACTGG TCTGATCTTA TCGAGTATGT GATTCACAAG 
    TTCATGAATA CTCCTCCGAA GTCGCCACTC ACCATTCCTA CAACTTCCTT GACGGAAATG 
    ACCAAGGAAC ACAACCAGGA GCATACCGTT TGCTCTTGGT CGCAGGAGGA AATGGACACA 
    CTTTATTGGT ATTATGTGCA GAGCAAGAAG AACAACGATA TTGTGGGAAA GATAGTTAAG 
    CTCTTCAGCA ACAACGGCAA CAAGCTGAAA ACCAGGATTT CTATTATCCA ACAACTTTTG 
    CAACAAGACA TTATCACCCT GTTGGAATAC GATGACCTGA TGAAGTTCGA GGATGCGGAG 
    TATCAGAGAA CTTTGCTGAC AACTCCCACT TCCGCAACAA CAGAGTCTGG AATTGAGATT 
    AAGGAGTGCG CCTACGGCAA ACCCTCAGAT GATGTTCAGA TCCTGCTGGA CCTGATCATT 
    AAGGAAAACA AGGCGCAGCA TTTGTTATGG CTGCAAAGGA TCCTCATTGA GTGCTGCTTC 
    GTTAAACTGA CCCTGCGGAG TGGTCTCAAG GTTCCGGAAG GCGATCACAT CATGGAGCCG 
    GTGGCCTACC ACTGCATCTG CAAGCAGAAG TCCATTCCGG TGGTGCAGTG GAACAACGAG 
    CAATCCACTA CGATGCTGTA CCAGCCTTTT GTTCTCCTGC TCCACAAGCT GGGCATTCAG 
    CTGCCGGCGG ACGCGGGCTC GATCTTCGCC AGAATTCCGG ACTACTGGAC ACCGGAGACA 
    ATGTACGGAC TCGCCAAAAA GCTGGGACCG CTGGACAAAC GTGAGTTAAA GTCAACCACA 
    GAAAAAAACA ACCCATTTGT CATTCCACAA AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001033872.2
    CDS675..4070
    Translation

    Target ORF information:

    RefSeq Version NM_001038783.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster timeless (tim), transcript variant M, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001038783.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
    CAAAAACTAC TTAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
    AGTAATACCT CGCCCCCCAA ACAGGGCAGT GGCGATTCCA GCCCCATGCT GACCTCTGAT 
    CCTACCTCTG ATTCCTCGGA CAATGGCAGC AATGGCCGTG GCATGGGCGG TGGCATGCGG 
    GAAGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAGG GTCAGGAGTA TCAGAACGCC 
    ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
    AACGACAGCG AGCAGCCTGG ATCGCCGGAG CAATCGCAGC CCGCCGGCGA GTCCATGGAC 
    GATGGAGATT ACGAGGACCA GAGACACAGG CAACTGAACG AGCATGGCGA GGAGGATGAA 
    GATGAGGACG AAGTGGAGGA GGAAGAGTAC CTACAATTGG GCCCAGCCTC GGAGCCCCTA 
    AACTTAACAC AACAACCAGC TGACAAGGTC AACAACACTA CCAACCCAAC GTCCAGTGCG 
    CCACAAGGCT GCCTGGGCAA TGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
    TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
    TCTGCGGTCA AATTGGGCCA AAAGTCCCCA CATGCCGGCC AGCTCCAGCT GACCAAGGGC 
    AAGTGTTGCC CACAGAAGCG GGAATGCCCC TCCTCGCAGT CGGAGCTATC GGATTGCGGT 
    TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGATGATGGG 
    CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
    CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTTAGAC GCAAAAAACT GGTCAAGCGC 
    AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTACAGC ACACCCCCAC CGATGATGAC 
    ATCTCCAATC TGCTGAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
    GTGGAGGAAC TGCACATGCA ACTGCTTTCC AATGCGAAGG TGCCCATTGA CACATCGCAC 
    TTCTTCTGGC TGGTCACCTA CTTCCTGAAG TTTGCCGCCC AACTGGAGCT GGATATGGAG 
    CACATCGACA CTATTCTCAC CTACGATGTT TTGAGCTACT TGACCTATGA GGGTGTGTCC 
    CTATGTGAGC AACTGGAACT GAATGCCCGA CAGGAGGGCA GTGACCTGAA GCCCTATCTA 
    AGGCGAATGC ACTTGGTGGT GACGGCCATC CGGGAGTTCC TCCAGGCCAT TGATACGTAC 
    AACAAAGTGA CTCATCTGAA CGAGGACGAC AAAGCCCATT TGAGGCAGCT TCAGCTGCAG 
    ATTAGCGAAA TGTCCGATCT GAGGTGCCTT TTTGTGCTTC TGCTGAGGCG TTTCAATCCC 
    AGCATTCATT CCAAGCAGTA TCTTCAGGAT CTGGTGGTTA CCAATCACAT CCTCCTACTC 
    ATTCTGGACA GTTCGGCCAA ACTTGGTGGA TGTCAAACCA TTCGCCTGTC GGAGCACATA 
    ACACAGTTTG CCACGCTGGA GGTGATGCAC TACTATGGCA TTCTGTTGGA GGACTTCAAC 
    AACAACGGAG AGTTTGTCAA TGACTGCATC TTCACCATGA TGCATCACAT CGGTGGCGAT 
    CTGGGCCAGA TTGGGGTTCT ATTTCAACCA ATTATTTTGA AAACCTATTC AAGAATTTGG 
    GAAGCGGACT ATGAACTGTG CGATGACTGG TCTGATCTTA TCGAGTATGT GATTCACAAG 
    TTCATGAATA CTCCTCCGAA GTCGCCACTC ACCATTCCTA CAACTTCCTT GACGGAAATG 
    ACCAAGGAAC ACAACCAGGA GCATACCGTT TGCTCTTGGT CGCAGGAGGA AATGGACACA 
    CTTTATTGGT ATTATGTGCA GAGCAAGAAG AACAACGATA TTGTGGGAAA GATAGTTAAG 
    CTCTTCAGCA ACAACGGCAA CAAGCTGAAA ACCAGGATTT CTATTATCCA ACAACTTTTG 
    CAACAAGACA TTATCACCCT GTTGGAATAC GATGACCTGA TGAAGTTCGA GGATGCGGAG 
    TATCAGAGAA CTTTGCTGAC AACTCCCACT TCCGCAACAA CAGAGTCTGG AATTGAGATT 
    AAGGAGTGCG CCTACGGCAA ACCCTCAGAT GATGTTCAGA TCCTGCTGGA CCTGATCATT 
    AAGGAAAACA AGGCGCAGCA TTTGTTATGG CTGCAAAGGA TCCTCATTGA GTGCTGCTTC 
    GTTAAACTGA CCCTGCGGAG TGGTCTCAAG GTTCCGGAAG GCGATCACAT CATGGAGCCG 
    GTGGCCTACC ACTGCATCTG CAAGCAGAAG TCCATTCCGG TGGTGCAGTG GAACAACGAG 
    CAATCCACTA CGATGCTGTA CCAGCCTTTT GTTCTCCTGC TCCACAAGCT GGGCATTCAG 
    CTGCCGGCGG ACGCGGGCTC GATCTTCGCC AGAATTCCGG ACTACTGGAC ACCGGAGACA 
    ATGTACGGAC TCGCCAAAAA GCTGGGACCG CTGGACAAAC GTGAGTTAAA GTCAACCACA 
    GAAAAAAACA ACCCATTTGT CATTCCACAA AGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    tim ( NM_001014463.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014463.2 Drosophila melanogaster timeless (tim), transcript variant S, mRNA.
    Price & Availability↑
    CloneID OFa13653
    Clone ID Related Accession (Same CDS sequence) NM_001014463.4
    Accession Version NM_001014463.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product timeless, isoform S
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001014463.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGG CAGTGGCGAT 
    TCCAGCCCCA TGCTGACCTC TGATCCTACC TCTGATTCCT CGGACAATGG CAGCAATGGC 
    CGTGGCATGG GCGGTGGCAT GCGGGAAGGA ACAGCGGCCA CTTTGCAGGA GGTGAGCCGC 
    AAGGGTCAGG AGTATCAGAA CGCCATGGCC AGAGTGCCAG CGGATAAGCC CGATGGCTCC 
    GAAGAGGCCA GCGATATGAC GGGGAACGAC AGCGAGCAGC CTGGATCGCC GGAGCAATCG 
    CAGCCCGCCG GCGAGTCCAT GGACGATGGA GATTACGAGG ACCAGAGACA CAGGCAACTG 
    AACGAGCATG GCGAGGAGGA TGAAGATGAG GACGAAGTGG AGGAGGAAGA GTACCTACAA 
    TTGGGCCCAG CCTCGGAGCC CCTAAACTTA ACACAACAAC CAGCTGACAA GGTCAACAAC 
    ACTACCAACC CAACGTCCAG TGCGCCACAA GGCTGCCTGG GCAATGAGCC ATTCAAGCCA 
    CCACCTCCTC TGCCAGTCAG AGCCTCCACC TCGGCACACG CTCAAATGCA GAAGTTCAAC 
    GAATCGTCCT ACGCGTCCCA CGTATCTGCG GTCAAATTGG GCCAAAAGTC CCCACATGCC 
    GGCCAGCTCC AGCTGACCAA GGGCAAGTGT TGCCCACAGA AGCGGGAATG CCCCTCCTCG 
    CAGTCGGAGC TATCGGATTG CGGTTATGGC ACCCAGGTGG AAAATCAGGA ATCCATTTCC 
    ACCTCCAGCA ACGACGATGA TGGGCCGCAG GGCAAGCCGC AGCACCAGAA GCCTCCGTGT 
    AACACGAAGC CACGGAATAA ACCACGGACG ATTATGTCGC CAATGGACAA AAAGGAGCTT 
    AGACGCAAAA AACTGGTCAA GCGCAGCAAA AGCAGCCTCA TCAACATGAA GGGTCTGGTA 
    CAGCACACCC CCACCGATGA TGACATCTCC AATCTGCTGA AGGAATTCAC CGTGGATTTC 
    CTCCTCAAGG GTTACAGCTA TCTGGTGGAG GAACTGCACA TGCAACTGCT TTCCAATGCG 
    AAGGTGCCCA TTGACACATC GCACTTCTTC TGGCTGGTCA CCTACTTCCT GAAGTTTGCC 
    GCCCAACTGG AGCTGGATAT GGAGCACATC GACACTATTC TCACCTACGA TGTTTTGAGC 
    TACTTGACCT ATGAGGGTGT GTCCCTATGT GAGCAACTGG AACTGAATGC CCGACAGGAG 
    GGCAGTGACC TGAAGCCCTA TCTAAGGCGA ATGCACTTGG TGGTGACGGC CATCCGGGAG 
    TTCCTCCAGG CCATTGATAC GTACAACAAA GTGACTCATC TGAACGAGGA CGACAAAGCC 
    CATTTGAGGC AGCTTCAGCT GCAGATTAGC GAAATGTCCG ATCTGAGGTG CCTTTTTGTG 
    CTTCTGCTGA GGCGTTTCAA TCCCAGCATT CATTCCAAGC AGTATCTTCA GGATCTGGTG 
    GTTACCAATC ACATCCTCCT ACTCATTCTG GACAGTTCGG CCAAACTTGG TGGATGTCAA 
    ACCATTCGCC TGTCGGAGCA CATAACACAG TTTGCCACGC TGGAGGTGAT GCACTACTAT 
    GGCATTCTGT TGGAGGACTT CAACAACAAC GGAGAGTTTG TCAATGACTG CATCTTCACC 
    ATGATGCATC ACATCGGTGG CGATCTGGGC CAGATTGGGG TTCTATTTCA ACCAATTATT 
    TTGAAAACCT ATTCAAGAAT TTGGGAAGCG GACTATGAAC TGTGCGATGA CTGGTCTGAT 
    CTTATCGAGT ATGTGATTCA CAAGTTCATG AATACTCCTC CGAAGTCGCC ACTCACCATT 
    CCTACAACTT CCTTGACGGA AATGACCAAG GAACACAACC AGGAGCATAC CGTTTGCTCT 
    TGGTCGCAGG AGGAAATGGA CACACTTTAT TGGTATTATG TGCAGAGCAA GAAGAACAAC 
    GATATTGTGG GAAAGATAGT TAAGCTCTTC AGCAACAACG GCAACAAGCT GAAAACCAGG 
    ATTTCTATTA TCCAACAACT TTTGCAACAA GACATTATCA CCCTGTTGGA ATACGATGAC 
    CTGATGAAGT TCGAGGATGC GGAGTATCAG AGAACTTTGC TGACAACTCC CACTTCCGCA 
    ACAACAGAGT CTGGAATTGA GATTAAGGAG TGCGCCTACG GCAAACCCTC AGATGATGTT 
    CAGATCCTGC TGGACCTGAT CATTAAGGAA AACAAGGCGC AGCATTTGTT ATGGCTGCAA 
    AGGATCCTCA TTGAGTGCTG CTTCGTTAAA CTGACCCTGC GGAGTGGTCT CAAGGTTCCG 
    GAAGGCGATC ACATCATGGA GCCGGTGGCC TACCACTGCA TCTGCAAGCA GAAGTCCATT 
    CCGGTGGTGC AGTGGAACAA CGAGCAATCC ACTACGATGC TGTACCAGCC TTTTGTTCTC 
    CTGCTCCACA AGCTGGGCAT TCAGCTGCCG GCGGACGCGG GCTCGATCTT CGCCAGAATT 
    CCGGACTACT GGACACCGGA GACAATGTAC GGACTCGCCA AAAAGCTGGG ACCGCTGGAC 
    AAACGTGAGT TAAAGTCAAC CACAGAAAAA AACAACCCAT TTGTCATTCC ACAAAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001014463.2
    CDS344..3643
    Translation

    Target ORF information:

    RefSeq Version NM_001014463.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster timeless (tim), transcript variant S, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001014463.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGG CAGTGGCGAT 
    TCCAGCCCCA TGCTGACCTC TGATCCTACC TCTGATTCCT CGGACAATGG CAGCAATGGC 
    CGTGGCATGG GCGGTGGCAT GCGGGAAGGA ACAGCGGCCA CTTTGCAGGA GGTGAGCCGC 
    AAGGGTCAGG AGTATCAGAA CGCCATGGCC AGAGTGCCAG CGGATAAGCC CGATGGCTCC 
    GAAGAGGCCA GCGATATGAC GGGGAACGAC AGCGAGCAGC CTGGATCGCC GGAGCAATCG 
    CAGCCCGCCG GCGAGTCCAT GGACGATGGA GATTACGAGG ACCAGAGACA CAGGCAACTG 
    AACGAGCATG GCGAGGAGGA TGAAGATGAG GACGAAGTGG AGGAGGAAGA GTACCTACAA 
    TTGGGCCCAG CCTCGGAGCC CCTAAACTTA ACACAACAAC CAGCTGACAA GGTCAACAAC 
    ACTACCAACC CAACGTCCAG TGCGCCACAA GGCTGCCTGG GCAATGAGCC ATTCAAGCCA 
    CCACCTCCTC TGCCAGTCAG AGCCTCCACC TCGGCACACG CTCAAATGCA GAAGTTCAAC 
    GAATCGTCCT ACGCGTCCCA CGTATCTGCG GTCAAATTGG GCCAAAAGTC CCCACATGCC 
    GGCCAGCTCC AGCTGACCAA GGGCAAGTGT TGCCCACAGA AGCGGGAATG CCCCTCCTCG 
    CAGTCGGAGC TATCGGATTG CGGTTATGGC ACCCAGGTGG AAAATCAGGA ATCCATTTCC 
    ACCTCCAGCA ACGACGATGA TGGGCCGCAG GGCAAGCCGC AGCACCAGAA GCCTCCGTGT 
    AACACGAAGC CACGGAATAA ACCACGGACG ATTATGTCGC CAATGGACAA AAAGGAGCTT 
    AGACGCAAAA AACTGGTCAA GCGCAGCAAA AGCAGCCTCA TCAACATGAA GGGTCTGGTA 
    CAGCACACCC CCACCGATGA TGACATCTCC AATCTGCTGA AGGAATTCAC CGTGGATTTC 
    CTCCTCAAGG GTTACAGCTA TCTGGTGGAG GAACTGCACA TGCAACTGCT TTCCAATGCG 
    AAGGTGCCCA TTGACACATC GCACTTCTTC TGGCTGGTCA CCTACTTCCT GAAGTTTGCC 
    GCCCAACTGG AGCTGGATAT GGAGCACATC GACACTATTC TCACCTACGA TGTTTTGAGC 
    TACTTGACCT ATGAGGGTGT GTCCCTATGT GAGCAACTGG AACTGAATGC CCGACAGGAG 
    GGCAGTGACC TGAAGCCCTA TCTAAGGCGA ATGCACTTGG TGGTGACGGC CATCCGGGAG 
    TTCCTCCAGG CCATTGATAC GTACAACAAA GTGACTCATC TGAACGAGGA CGACAAAGCC 
    CATTTGAGGC AGCTTCAGCT GCAGATTAGC GAAATGTCCG ATCTGAGGTG CCTTTTTGTG 
    CTTCTGCTGA GGCGTTTCAA TCCCAGCATT CATTCCAAGC AGTATCTTCA GGATCTGGTG 
    GTTACCAATC ACATCCTCCT ACTCATTCTG GACAGTTCGG CCAAACTTGG TGGATGTCAA 
    ACCATTCGCC TGTCGGAGCA CATAACACAG TTTGCCACGC TGGAGGTGAT GCACTACTAT 
    GGCATTCTGT TGGAGGACTT CAACAACAAC GGAGAGTTTG TCAATGACTG CATCTTCACC 
    ATGATGCATC ACATCGGTGG CGATCTGGGC CAGATTGGGG TTCTATTTCA ACCAATTATT 
    TTGAAAACCT ATTCAAGAAT TTGGGAAGCG GACTATGAAC TGTGCGATGA CTGGTCTGAT 
    CTTATCGAGT ATGTGATTCA CAAGTTCATG AATACTCCTC CGAAGTCGCC ACTCACCATT 
    CCTACAACTT CCTTGACGGA AATGACCAAG GAACACAACC AGGAGCATAC CGTTTGCTCT 
    TGGTCGCAGG AGGAAATGGA CACACTTTAT TGGTATTATG TGCAGAGCAA GAAGAACAAC 
    GATATTGTGG GAAAGATAGT TAAGCTCTTC AGCAACAACG GCAACAAGCT GAAAACCAGG 
    ATTTCTATTA TCCAACAACT TTTGCAACAA GACATTATCA CCCTGTTGGA ATACGATGAC 
    CTGATGAAGT TCGAGGATGC GGAGTATCAG AGAACTTTGC TGACAACTCC CACTTCCGCA 
    ACAACAGAGT CTGGAATTGA GATTAAGGAG TGCGCCTACG GCAAACCCTC AGATGATGTT 
    CAGATCCTGC TGGACCTGAT CATTAAGGAA AACAAGGCGC AGCATTTGTT ATGGCTGCAA 
    AGGATCCTCA TTGAGTGCTG CTTCGTTAAA CTGACCCTGC GGAGTGGTCT CAAGGTTCCG 
    GAAGGCGATC ACATCATGGA GCCGGTGGCC TACCACTGCA TCTGCAAGCA GAAGTCCATT 
    CCGGTGGTGC AGTGGAACAA CGAGCAATCC ACTACGATGC TGTACCAGCC TTTTGTTCTC 
    CTGCTCCACA AGCTGGGCAT TCAGCTGCCG GCGGACGCGG GCTCGATCTT CGCCAGAATT 
    CCGGACTACT GGACACCGGA GACAATGTAC GGACTCGCCA AAAAGCTGGG ACCGCTGGAC 
    AAACGTGAGT TAAAGTCAAC CACAGAAAAA AACAACCCAT TTGTCATTCC ACAAAGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    tim ( NM_001144308.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137780.2 Drosophila melanogaster timeless (tim), transcript variant R, mRNA.
    Price & Availability↑
    CloneID OFa13650
    Clone ID Related Accession (Same CDS sequence) NM_001144308.3
    Accession Version NM_001144308.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product timeless, isoform R
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001144308.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGG CAGTGGCGAT 
    TCCAGCCCCA TGCTGACCTC TGATCCTACC TCTGATTCCT CGGACAATGG CAGCAATGGC 
    CGTGGCATGG GCGGTGGCAT GCGGGAAGGA ACAGCGGCCA CTTTGCAGGA GGTGAGCCGC 
    AAGGGTCAGG AGTATCAGAA CGCCATGGCC AGAGTGCCAG CGGATAAGCC CGATGGCTCC 
    GAAGAGGCCA GCGATATGAC GGGGAACGAC AGCGAGCAGC CTGGATCGCC GGAGCAATCG 
    CAGCCCGCCG GCGAGTCCAT GGACGATGGA GATTACGAGG ACCAGAGACA CAGGCAACTG 
    AACGAGCATG GCGAGGAGGA TGAAGATGAG GACGAAGTGG AGGAGGAAGA GTACCTACAA 
    TTGGGCCCAG CCTCGGAGCC CCTAAACTTA ACACAACAAC CAGCTGACAA GGTCAACAAC 
    ACTACCAACC CAACGTCCAG TGCGCCACAA GGCTGCCTGG GCAATGAGCC ATTCAAGCCA 
    CCACCTCCTC TGCCAGTCAG AGCCTCCACC TCGGCACACG CTCAAATGCA GAAGTTCAAC 
    GAATCGTCCT ACGCGTCCCA CGTATCTGCG GTCAAATTGG GCCAAAAGTC CCCACATGCC 
    GGCCAGCTCC AGCTGACCAA GGGCAAGTGT TGCCCACAGA AGCGGGAATG CCCCTCCTCG 
    CAGTCGGAGC TATCGGATTG CGGTTATGGC ACCCAGGTGG AAAATCAGGA ATCCATTTCC 
    ACCTCCAGCA ACGACGATGA TGGGCCGCAG GGCAAGCCGC AGCACCAGAA GCCTCCGTGT 
    AACACGAAGC CACGGAATAA ACCACGGACG ATTATGTCGC CAATGGACAA AAAGGAGCTT 
    AGACGCAAAA AACTGGTCAA GCGCAGCAAA AGCAGCCTCA TCAACATGAA GGGTCTGGTA 
    CAGCACACCC CCACCGATGA TGACATCTCC AATCTGCTGA AGGAATTCAC CGTGGATTTC 
    CTCCTCAAGG GTTACAGCTA TCTGGTGGAG GAACTGCACA TGCAACTGCT TTCCAATGCG 
    AAGGTGCCCA TTGACACATC GCACTTCTTC TGGCTGGTCA CCTACTTCCT GAAGTTTGCC 
    GCCCAACTGG AGCTGGATAT GGAGCACATC GACACTATTC TCACCTACGA TGTTTTGAGC 
    TACTTGACCT ATGAGGGTGT GTCCCTATGT GAGCAACTGG AACTGAATGC CCGACAGGAG 
    GGCAGTGACC TGAAGCCCTA TCTAAGGCGA ATGCACTTGG TGGTGACGGC CATCCGGGAG 
    TTCCTCCAGG CCATTGATAC GTACAACAAA GTGACTCATC TGAACGAGGA CGACAAAGCC 
    CATTTGAGGC AGCTTCAGCT GCAGATTAGC GAAATGTCCG ATCTGAGGTG CCTTTTTGTG 
    CTTCTGCTGA GGCGTTTCAA TCCCAGCATT CATTCCAAGC AGTATCTTCA GGATCTGGTG 
    GTTACCAATC ACATCCTCCT ACTCATTCTG GACAGTTCGG CCAAACTTGG TGGATGTCAA 
    ACCATTCGCC TGTCGGAGCA CATAACACAG TTTGCCACGC TGGAGGTGAT GCACTACTAT 
    GGCATTCTGT TGGAGGACTT CAACAACAAC GGAGAGTTTG TCAATGACTG CATCTTCACC 
    ATGATGCATC ACATCGGTGG CGATCTGGGC CAGATTGGGG TTCTATTTCA ACCAATTATT 
    TTGAAAACCT ATTCAAGAAT TTGGGAAGCG GACTATGAAC TGTGCGATGA CTGGTCTGAT 
    CTTATCGAGT ATGTGATTCA CAAGTTCATG AATACTCCTC CGAAGTCGCC ACTCACCATT 
    CCTACAACTT CCTTGACGGA AATGACCAAG GAACACAACC AGGAGCATAC CGTTTGCTCT 
    TGGTCGCAGG AGGAAATGGA CACACTTTAT TGGTATTATG TGCAGAGCAA GAAGAACAAC 
    GATATTGTGG GAAAGATAGT TAAGCTCTTC AGCAACAACG GCAACAAGCT GAAAACCAGG 
    ATTTCTATTA TCCAACAACT TTTGCAACAA GACATTATCA CCCTGTTGGA ATACGATGAC 
    CTGATGAAGT TCGAGGATGC GGAGTATCAG AGAACTTTGC TGACAACTCC CACTTCCGCA 
    ACAACAGAGT CTGGAATTGA GATTAAGGAG TGCGCCTACG GCAAACCCTC AGATGATGTT 
    CAGATCCTGC TGGACCTGAT CATTAAGGAA AACAAGGCGC AGCATTTGTT ATGGCTGCAA 
    AGGATCCTCA TTGAGTGCTG CTTCGTTAAA CTGACCCTGC GGAGTGGTCT CAAGGTTCCG 
    GAAGGCGATC ACATCATGGA GCCGGTGGCC TACCACTGCA TCTGCAAGCA GAAGTCCATT 
    CCGGTGGTGC AGTGGAACAA CGAGCAATCC ACTACGATGC TGTACCAGCC TTTTGTTCTC 
    CTGCTCCACA AGCTGGGCAT TCAGCTGCCG GCGGACGCGG GCTCGATCTT CGCCAGAATT 
    CCGGACTACT GGACACCGGA GACAATGTAC GGACTCGCCA AAAAGCTGGG ACCGCTGGAC 
    AAACTCAACC TCAAGTTCGA CGCCAGTGAA CTGGAGGATG CGACGGCGTC GAGTCCGTCG 
    CGTTACCACC ACACCGGACC CCGCAACTCG CTCAGCTCGG TAAGCAGCCT GGACGTGGAT 
    CTCGGCGATA CCGAGGAGCT GGCCCTTATA CCCGAGGTGG ATGCGGCCGT GGAGAAGGCA 
    CACGCCATGG CATCCACGCC ATCGCCCAGC GAGATTTTCG CGGTTCCCAA GACGAAGCAC 
    TGCAACTCGA TCATCAGATA CACACCAGAT CCCACGCCTC CAGTGCCCAA CTGGCTGCAG 
    TTGGTCATGC GCAGCAAATG CAATCATCGC ACAGGTCCGT CTGGTGATCC CAGCGATTGC 
    ATTGGCTCCT CGTCGACAAC CGTGGACGAT GAGGGATTTG GCAAGTCCAT CAGTGCAGCC 
    ACTTCGCAGG CGGCGAGCAC CTCCATGAGC ACGGTTAATC CCACAACCAC TTTGAGCCTG 
    AACATGCTAA ACACCTTCAT GGGAAGCCAC AACGAGAACA GCAGCAGTTC TGGTTGCGGG 
    GGCACCGTCT CCTCCCTGTC CATGGTGGCT CTGATGAGCA CCGGCGCGGC AGGCGGAGGA 
    GGTAACACCT CCGGGCTGGA AATGGATGTG GACGCCTCCA TGAAGTCCTC GTTCGAGCGG 
    CTGGAGGTAA ACGGATCGCA CTTCTCGCGG GCCAACAACT TGGACCAGGA GTACAGTGCC 
    ATGGTGGCAT CTGTGTACGA AAAGGAGAAG GAATTAAACA GCGACAATGT CTCTTTGGCC 
    TCGGACCTGA CCAGAATGTA TGTGAGCGAT GAGGACGATC GACTTGAGCG AACCGAGATC 
    CGGGTGCCCC ACTATCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137780.2
    CDS387..4487
    Translation

    Target ORF information:

    RefSeq Version NM_001144308.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster timeless (tim), transcript variant R, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001144308.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGG CAGTGGCGAT 
    TCCAGCCCCA TGCTGACCTC TGATCCTACC TCTGATTCCT CGGACAATGG CAGCAATGGC 
    CGTGGCATGG GCGGTGGCAT GCGGGAAGGA ACAGCGGCCA CTTTGCAGGA GGTGAGCCGC 
    AAGGGTCAGG AGTATCAGAA CGCCATGGCC AGAGTGCCAG CGGATAAGCC CGATGGCTCC 
    GAAGAGGCCA GCGATATGAC GGGGAACGAC AGCGAGCAGC CTGGATCGCC GGAGCAATCG 
    CAGCCCGCCG GCGAGTCCAT GGACGATGGA GATTACGAGG ACCAGAGACA CAGGCAACTG 
    AACGAGCATG GCGAGGAGGA TGAAGATGAG GACGAAGTGG AGGAGGAAGA GTACCTACAA 
    TTGGGCCCAG CCTCGGAGCC CCTAAACTTA ACACAACAAC CAGCTGACAA GGTCAACAAC 
    ACTACCAACC CAACGTCCAG TGCGCCACAA GGCTGCCTGG GCAATGAGCC ATTCAAGCCA 
    CCACCTCCTC TGCCAGTCAG AGCCTCCACC TCGGCACACG CTCAAATGCA GAAGTTCAAC 
    GAATCGTCCT ACGCGTCCCA CGTATCTGCG GTCAAATTGG GCCAAAAGTC CCCACATGCC 
    GGCCAGCTCC AGCTGACCAA GGGCAAGTGT TGCCCACAGA AGCGGGAATG CCCCTCCTCG 
    CAGTCGGAGC TATCGGATTG CGGTTATGGC ACCCAGGTGG AAAATCAGGA ATCCATTTCC 
    ACCTCCAGCA ACGACGATGA TGGGCCGCAG GGCAAGCCGC AGCACCAGAA GCCTCCGTGT 
    AACACGAAGC CACGGAATAA ACCACGGACG ATTATGTCGC CAATGGACAA AAAGGAGCTT 
    AGACGCAAAA AACTGGTCAA GCGCAGCAAA AGCAGCCTCA TCAACATGAA GGGTCTGGTA 
    CAGCACACCC CCACCGATGA TGACATCTCC AATCTGCTGA AGGAATTCAC CGTGGATTTC 
    CTCCTCAAGG GTTACAGCTA TCTGGTGGAG GAACTGCACA TGCAACTGCT TTCCAATGCG 
    AAGGTGCCCA TTGACACATC GCACTTCTTC TGGCTGGTCA CCTACTTCCT GAAGTTTGCC 
    GCCCAACTGG AGCTGGATAT GGAGCACATC GACACTATTC TCACCTACGA TGTTTTGAGC 
    TACTTGACCT ATGAGGGTGT GTCCCTATGT GAGCAACTGG AACTGAATGC CCGACAGGAG 
    GGCAGTGACC TGAAGCCCTA TCTAAGGCGA ATGCACTTGG TGGTGACGGC CATCCGGGAG 
    TTCCTCCAGG CCATTGATAC GTACAACAAA GTGACTCATC TGAACGAGGA CGACAAAGCC 
    CATTTGAGGC AGCTTCAGCT GCAGATTAGC GAAATGTCCG ATCTGAGGTG CCTTTTTGTG 
    CTTCTGCTGA GGCGTTTCAA TCCCAGCATT CATTCCAAGC AGTATCTTCA GGATCTGGTG 
    GTTACCAATC ACATCCTCCT ACTCATTCTG GACAGTTCGG CCAAACTTGG TGGATGTCAA 
    ACCATTCGCC TGTCGGAGCA CATAACACAG TTTGCCACGC TGGAGGTGAT GCACTACTAT 
    GGCATTCTGT TGGAGGACTT CAACAACAAC GGAGAGTTTG TCAATGACTG CATCTTCACC 
    ATGATGCATC ACATCGGTGG CGATCTGGGC CAGATTGGGG TTCTATTTCA ACCAATTATT 
    TTGAAAACCT ATTCAAGAAT TTGGGAAGCG GACTATGAAC TGTGCGATGA CTGGTCTGAT 
    CTTATCGAGT ATGTGATTCA CAAGTTCATG AATACTCCTC CGAAGTCGCC ACTCACCATT 
    CCTACAACTT CCTTGACGGA AATGACCAAG GAACACAACC AGGAGCATAC CGTTTGCTCT 
    TGGTCGCAGG AGGAAATGGA CACACTTTAT TGGTATTATG TGCAGAGCAA GAAGAACAAC 
    GATATTGTGG GAAAGATAGT TAAGCTCTTC AGCAACAACG GCAACAAGCT GAAAACCAGG 
    ATTTCTATTA TCCAACAACT TTTGCAACAA GACATTATCA CCCTGTTGGA ATACGATGAC 
    CTGATGAAGT TCGAGGATGC GGAGTATCAG AGAACTTTGC TGACAACTCC CACTTCCGCA 
    ACAACAGAGT CTGGAATTGA GATTAAGGAG TGCGCCTACG GCAAACCCTC AGATGATGTT 
    CAGATCCTGC TGGACCTGAT CATTAAGGAA AACAAGGCGC AGCATTTGTT ATGGCTGCAA 
    AGGATCCTCA TTGAGTGCTG CTTCGTTAAA CTGACCCTGC GGAGTGGTCT CAAGGTTCCG 
    GAAGGCGATC ACATCATGGA GCCGGTGGCC TACCACTGCA TCTGCAAGCA GAAGTCCATT 
    CCGGTGGTGC AGTGGAACAA CGAGCAATCC ACTACGATGC TGTACCAGCC TTTTGTTCTC 
    CTGCTCCACA AGCTGGGCAT TCAGCTGCCG GCGGACGCGG GCTCGATCTT CGCCAGAATT 
    CCGGACTACT GGACACCGGA GACAATGTAC GGACTCGCCA AAAAGCTGGG ACCGCTGGAC 
    AAACTCAACC TCAAGTTCGA CGCCAGTGAA CTGGAGGATG CGACGGCGTC GAGTCCGTCG 
    CGTTACCACC ACACCGGACC CCGCAACTCG CTCAGCTCGG TAAGCAGCCT GGACGTGGAT 
    CTCGGCGATA CCGAGGAGCT GGCCCTTATA CCCGAGGTGG ATGCGGCCGT GGAGAAGGCA 
    CACGCCATGG CATCCACGCC ATCGCCCAGC GAGATTTTCG CGGTTCCCAA GACGAAGCAC 
    TGCAACTCGA TCATCAGATA CACACCAGAT CCCACGCCTC CAGTGCCCAA CTGGCTGCAG 
    TTGGTCATGC GCAGCAAATG CAATCATCGC ACAGGTCCGT CTGGTGATCC CAGCGATTGC 
    ATTGGCTCCT CGTCGACAAC CGTGGACGAT GAGGGATTTG GCAAGTCCAT CAGTGCAGCC 
    ACTTCGCAGG CGGCGAGCAC CTCCATGAGC ACGGTTAATC CCACAACCAC TTTGAGCCTG 
    AACATGCTAA ACACCTTCAT GGGAAGCCAC AACGAGAACA GCAGCAGTTC TGGTTGCGGG 
    GGCACCGTCT CCTCCCTGTC CATGGTGGCT CTGATGAGCA CCGGCGCGGC AGGCGGAGGA 
    GGTAACACCT CCGGGCTGGA AATGGATGTG GACGCCTCCA TGAAGTCCTC GTTCGAGCGG 
    CTGGAGGTAA ACGGATCGCA CTTCTCGCGG GCCAACAACT TGGACCAGGA GTACAGTGCC 
    ATGGTGGCAT CTGTGTACGA AAAGGAGAAG GAATTAAACA GCGACAATGT CTCTTTGGCC 
    TCGGACCTGA CCAGAATGTA TGTGAGCGAT GAGGACGATC GACTTGAGCG AACCGAGATC 
    CGGGTGCCCC ACTATCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    tim ( NM_164542.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722914.3 Drosophila melanogaster timeless (tim), transcript variant B, mRNA.
    Price & Availability↑
    CloneID OFa13651
    Clone ID Related Accession (Same CDS sequence) NM_164542.5 , NM_164540.4
    Accession Version NM_164542.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product timeless, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_164542.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
    CAAAAACTAC TTAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
    AGTAATACCT CGCCCCCCAA ACAGGGCAGT GGCGATTCCA GCCCCATGCT GACCTCTGAT 
    CCTACCTCTG ATTCCTCGGA CAATGGCAGC AATGGCCGTG GCATGGGCGG TGGCATGCGG 
    GAAGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAGG GTCAGGAGTA TCAGAACGCC 
    ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
    AACGACAGCG AGCAGCCTGG ATCGCCGGAG CAATCGCAGC CCGCCGGCGA GTCCATGGAC 
    GATGGAGATT ACGAGGACCA GAGACACAGG CAACTGAACG AGCATGGCGA GGAGGATGAA 
    GATGAGGACG AAGTGGAGGA GGAAGAGTAC CTACAATTGG GCCCAGCCTC GGAGCCCCTA 
    AACTTAACAC AACAACCAGC TGACAAGGTC AACAACACTA CCAACCCAAC GTCCAGTGCG 
    CCACAAGGCT GCCTGGGCAA TGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
    TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
    TCTGCGGTCA AATTGGGCCA AAAGTCCCCA CATGCCGGCC AGCTCCAGCT GACCAAGGGC 
    AAGTGTTGCC CACAGAAGCG GGAATGCCCC TCCTCGCAGT CGGAGCTATC GGATTGCGGT 
    TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGATGATGGG 
    CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
    CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTTAGAC GCAAAAAACT GGTCAAGCGC 
    AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTACAGC ACACCCCCAC CGATGATGAC 
    ATCTCCAATC TGCTGAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
    GTGGAGGAAC TGCACATGCA ACTGCTTTCC AATGCGAAGG TGCCCATTGA CACATCGCAC 
    TTCTTCTGGC TGGTCACCTA CTTCCTGAAG TTTGCCGCCC AACTGGAGCT GGATATGGAG 
    CACATCGACA CTATTCTCAC CTACGATGTT TTGAGCTACT TGACCTATGA GGGTGTGTCC 
    CTATGTGAGC AACTGGAACT GAATGCCCGA CAGGAGGGCA GTGACCTGAA GCCCTATCTA 
    AGGCGAATGC ACTTGGTGGT GACGGCCATC CGGGAGTTCC TCCAGGCCAT TGATACGTAC 
    AACAAAGTGA CTCATCTGAA CGAGGACGAC AAAGCCCATT TGAGGCAGCT TCAGCTGCAG 
    ATTAGCGAAA TGTCCGATCT GAGGTGCCTT TTTGTGCTTC TGCTGAGGCG TTTCAATCCC 
    AGCATTCATT CCAAGCAGTA TCTTCAGGAT CTGGTGGTTA CCAATCACAT CCTCCTACTC 
    ATTCTGGACA GTTCGGCCAA ACTTGGTGGA TGTCAAACCA TTCGCCTGTC GGAGCACATA 
    ACACAGTTTG CCACGCTGGA GGTGATGCAC TACTATGGCA TTCTGTTGGA GGACTTCAAC 
    AACAACGGAG AGTTTGTCAA TGACTGCATC TTCACCATGA TGCATCACAT CGGTGGCGAT 
    CTGGGCCAGA TTGGGGTTCT ATTTCAACCA ATTATTTTGA AAACCTATTC AAGAATTTGG 
    GAAGCGGACT ATGAACTGTG CGATGACTGG TCTGATCTTA TCGAGTATGT GATTCACAAG 
    TTCATGAATA CTCCTCCGAA GTCGCCACTC ACCATTCCTA CAACTTCCTT GACGGAAATG 
    ACCAAGGAAC ACAACCAGGA GCATACCGTT TGCTCTTGGT CGCAGGAGGA AATGGACACA 
    CTTTATTGGT ATTATGTGCA GAGCAAGAAG AACAACGATA TTGTGGGAAA GATAGTTAAG 
    CTCTTCAGCA ACAACGGCAA CAAGCTGAAA ACCAGGATTT CTATTATCCA ACAACTTTTG 
    CAACAAGACA TTATCACCCT GTTGGAATAC GATGACCTGA TGAAGTTCGA GGATGCGGAG 
    TATCAGAGAA CTTTGCTGAC AACTCCCACT TCCGCAACAA CAGAGTCTGG AATTGAGATT 
    AAGGAGTGCG CCTACGGCAA ACCCTCAGAT GATGTTCAGA TCCTGCTGGA CCTGATCATT 
    AAGGAAAACA AGGCGCAGCA TTTGTTATGG CTGCAAAGGA TCCTCATTGA GTGCTGCTTC 
    GTTAAACTGA CCCTGCGGAG TGGTCTCAAG GTTCCGGAAG GCGATCACAT CATGGAGCCG 
    GTGGCCTACC ACTGCATCTG CAAGCAGAAG TCCATTCCGG TGGTGCAGTG GAACAACGAG 
    CAATCCACTA CGATGCTGTA CCAGCCTTTT GTTCTCCTGC TCCACAAGCT GGGCATTCAG 
    CTGCCGGCGG ACGCGGGCTC GATCTTCGCC AGAATTCCGG ACTACTGGAC ACCGGAGACA 
    ATGTACGGAC TCGCCAAAAA GCTGGGACCG CTGGACAAAC TCAACCTCAA GTTCGACGCC 
    AGTGAACTGG AGGATGCGAC GGCGTCGAGT CCGTCGCGTT ACCACCACAC CGGACCCCGC 
    AACTCGCTCA GCTCGGTAAG CAGCCTGGAC GTGGATCTCG GCGATACCGA GGAGCTGGCC 
    CTTATACCCG AGGTGGATGC GGCCGTGGAG AAGGCACACG CCATGGCATC CACGCCATCG 
    CCCAGCGAGA TTTTCGCGGT TCCCAAGACG AAGCACTGCA ACTCGATCAT CAGATACACA 
    CCAGATCCCA CGCCTCCAGT GCCCAACTGG CTGCAGTTGG TCATGCGCAG CAAATGCAAT 
    CATCGCACAG GTCCGTCTGG TGATCCCAGC GATTGCATTG GCTCCTCGTC GACAACCGTG 
    GACGATGAGG GATTTGGCAA GTCCATCAGT GCAGCCACTT CGCAGGCGGC GAGCACCTCC 
    ATGAGCACGG TTAATCCCAC AACCACTTTG AGCCTGAACA TGCTAAACAC CTTCATGGGA 
    AGCCACAACG AGAACAGCAG CAGTTCTGGT TGCGGGGGCA CCGTCTCCTC CCTGTCCATG 
    GTGGCTCTGA TGAGCACCGG CGCGGCAGGC GGAGGAGGTA ACACCTCCGG GCTGGAAATG 
    GATGTGGACG CCTCCATGAA GTCCTCGTTC GAGCGGCTGG AGGTAAACGG ATCGCACTTC 
    TCGCGGGCCA ACAACTTGGA CCAGGAGTAC AGTGCCATGG TGGCATCTGT GTACGAAAAG 
    GAGAAGGAAT TAAACAGCGA CAATGTCTCT TTGGCCTCGG ACCTGACCAG AATGTATGTG 
    AGCGATGAGG ACGATCGACT TGAGCGAACC GAGATCCGGG TGCCCCACTA TCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_722914.3
    CDS344..4540
    Translation

    Target ORF information:

    RefSeq Version NM_164542.5
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster timeless (tim), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_164542.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
    CAAAAACTAC TTAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
    AGTAATACCT CGCCCCCCAA ACAGGGCAGT GGCGATTCCA GCCCCATGCT GACCTCTGAT 
    CCTACCTCTG ATTCCTCGGA CAATGGCAGC AATGGCCGTG GCATGGGCGG TGGCATGCGG 
    GAAGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAGG GTCAGGAGTA TCAGAACGCC 
    ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
    AACGACAGCG AGCAGCCTGG ATCGCCGGAG CAATCGCAGC CCGCCGGCGA GTCCATGGAC 
    GATGGAGATT ACGAGGACCA GAGACACAGG CAACTGAACG AGCATGGCGA GGAGGATGAA 
    GATGAGGACG AAGTGGAGGA GGAAGAGTAC CTACAATTGG GCCCAGCCTC GGAGCCCCTA 
    AACTTAACAC AACAACCAGC TGACAAGGTC AACAACACTA CCAACCCAAC GTCCAGTGCG 
    CCACAAGGCT GCCTGGGCAA TGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
    TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
    TCTGCGGTCA AATTGGGCCA AAAGTCCCCA CATGCCGGCC AGCTCCAGCT GACCAAGGGC 
    AAGTGTTGCC CACAGAAGCG GGAATGCCCC TCCTCGCAGT CGGAGCTATC GGATTGCGGT 
    TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGATGATGGG 
    CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
    CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTTAGAC GCAAAAAACT GGTCAAGCGC 
    AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTACAGC ACACCCCCAC CGATGATGAC 
    ATCTCCAATC TGCTGAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
    GTGGAGGAAC TGCACATGCA ACTGCTTTCC AATGCGAAGG TGCCCATTGA CACATCGCAC 
    TTCTTCTGGC TGGTCACCTA CTTCCTGAAG TTTGCCGCCC AACTGGAGCT GGATATGGAG 
    CACATCGACA CTATTCTCAC CTACGATGTT TTGAGCTACT TGACCTATGA GGGTGTGTCC 
    CTATGTGAGC AACTGGAACT GAATGCCCGA CAGGAGGGCA GTGACCTGAA GCCCTATCTA 
    AGGCGAATGC ACTTGGTGGT GACGGCCATC CGGGAGTTCC TCCAGGCCAT TGATACGTAC 
    AACAAAGTGA CTCATCTGAA CGAGGACGAC AAAGCCCATT TGAGGCAGCT TCAGCTGCAG 
    ATTAGCGAAA TGTCCGATCT GAGGTGCCTT TTTGTGCTTC TGCTGAGGCG TTTCAATCCC 
    AGCATTCATT CCAAGCAGTA TCTTCAGGAT CTGGTGGTTA CCAATCACAT CCTCCTACTC 
    ATTCTGGACA GTTCGGCCAA ACTTGGTGGA TGTCAAACCA TTCGCCTGTC GGAGCACATA 
    ACACAGTTTG CCACGCTGGA GGTGATGCAC TACTATGGCA TTCTGTTGGA GGACTTCAAC 
    AACAACGGAG AGTTTGTCAA TGACTGCATC TTCACCATGA TGCATCACAT CGGTGGCGAT 
    CTGGGCCAGA TTGGGGTTCT ATTTCAACCA ATTATTTTGA AAACCTATTC AAGAATTTGG 
    GAAGCGGACT ATGAACTGTG CGATGACTGG TCTGATCTTA TCGAGTATGT GATTCACAAG 
    TTCATGAATA CTCCTCCGAA GTCGCCACTC ACCATTCCTA CAACTTCCTT GACGGAAATG 
    ACCAAGGAAC ACAACCAGGA GCATACCGTT TGCTCTTGGT CGCAGGAGGA AATGGACACA 
    CTTTATTGGT ATTATGTGCA GAGCAAGAAG AACAACGATA TTGTGGGAAA GATAGTTAAG 
    CTCTTCAGCA ACAACGGCAA CAAGCTGAAA ACCAGGATTT CTATTATCCA ACAACTTTTG 
    CAACAAGACA TTATCACCCT GTTGGAATAC GATGACCTGA TGAAGTTCGA GGATGCGGAG 
    TATCAGAGAA CTTTGCTGAC AACTCCCACT TCCGCAACAA CAGAGTCTGG AATTGAGATT 
    AAGGAGTGCG CCTACGGCAA ACCCTCAGAT GATGTTCAGA TCCTGCTGGA CCTGATCATT 
    AAGGAAAACA AGGCGCAGCA TTTGTTATGG CTGCAAAGGA TCCTCATTGA GTGCTGCTTC 
    GTTAAACTGA CCCTGCGGAG TGGTCTCAAG GTTCCGGAAG GCGATCACAT CATGGAGCCG 
    GTGGCCTACC ACTGCATCTG CAAGCAGAAG TCCATTCCGG TGGTGCAGTG GAACAACGAG 
    CAATCCACTA CGATGCTGTA CCAGCCTTTT GTTCTCCTGC TCCACAAGCT GGGCATTCAG 
    CTGCCGGCGG ACGCGGGCTC GATCTTCGCC AGAATTCCGG ACTACTGGAC ACCGGAGACA 
    ATGTACGGAC TCGCCAAAAA GCTGGGACCG CTGGACAAAC TCAACCTCAA GTTCGACGCC 
    AGTGAACTGG AGGATGCGAC GGCGTCGAGT CCGTCGCGTT ACCACCACAC CGGACCCCGC 
    AACTCGCTCA GCTCGGTAAG CAGCCTGGAC GTGGATCTCG GCGATACCGA GGAGCTGGCC 
    CTTATACCCG AGGTGGATGC GGCCGTGGAG AAGGCACACG CCATGGCATC CACGCCATCG 
    CCCAGCGAGA TTTTCGCGGT TCCCAAGACG AAGCACTGCA ACTCGATCAT CAGATACACA 
    CCAGATCCCA CGCCTCCAGT GCCCAACTGG CTGCAGTTGG TCATGCGCAG CAAATGCAAT 
    CATCGCACAG GTCCGTCTGG TGATCCCAGC GATTGCATTG GCTCCTCGTC GACAACCGTG 
    GACGATGAGG GATTTGGCAA GTCCATCAGT GCAGCCACTT CGCAGGCGGC GAGCACCTCC 
    ATGAGCACGG TTAATCCCAC AACCACTTTG AGCCTGAACA TGCTAAACAC CTTCATGGGA 
    AGCCACAACG AGAACAGCAG CAGTTCTGGT TGCGGGGGCA CCGTCTCCTC CCTGTCCATG 
    GTGGCTCTGA TGAGCACCGG CGCGGCAGGC GGAGGAGGTA ACACCTCCGG GCTGGAAATG 
    GATGTGGACG CCTCCATGAA GTCCTCGTTC GAGCGGCTGG AGGTAAACGG ATCGCACTTC 
    TCGCGGGCCA ACAACTTGGA CCAGGAGTAC AGTGCCATGG TGGCATCTGT GTACGAAAAG 
    GAGAAGGAAT TAAACAGCGA CAATGTCTCT TTGGCCTCGG ACCTGACCAG AATGTATGTG 
    AGCGATGAGG ACGATCGACT TGAGCGAACC GAGATCCGGG TGCCCCACTA TCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    tim ( NM_164540.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722912.3 Drosophila melanogaster timeless (tim), transcript variant P, mRNA.
    Price & Availability↑
    CloneID OFa13651
    Clone ID Related Accession (Same CDS sequence) NM_164542.5 , NM_164540.4
    Accession Version NM_164540.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product timeless, isoform P
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164540.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
    CAAAAACTAC TTAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
    AGTAATACCT CGCCCCCCAA ACAGGGCAGT GGCGATTCCA GCCCCATGCT GACCTCTGAT 
    CCTACCTCTG ATTCCTCGGA CAATGGCAGC AATGGCCGTG GCATGGGCGG TGGCATGCGG 
    GAAGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAGG GTCAGGAGTA TCAGAACGCC 
    ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
    AACGACAGCG AGCAGCCTGG ATCGCCGGAG CAATCGCAGC CCGCCGGCGA GTCCATGGAC 
    GATGGAGATT ACGAGGACCA GAGACACAGG CAACTGAACG AGCATGGCGA GGAGGATGAA 
    GATGAGGACG AAGTGGAGGA GGAAGAGTAC CTACAATTGG GCCCAGCCTC GGAGCCCCTA 
    AACTTAACAC AACAACCAGC TGACAAGGTC AACAACACTA CCAACCCAAC GTCCAGTGCG 
    CCACAAGGCT GCCTGGGCAA TGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
    TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
    TCTGCGGTCA AATTGGGCCA AAAGTCCCCA CATGCCGGCC AGCTCCAGCT GACCAAGGGC 
    AAGTGTTGCC CACAGAAGCG GGAATGCCCC TCCTCGCAGT CGGAGCTATC GGATTGCGGT 
    TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGATGATGGG 
    CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
    CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTTAGAC GCAAAAAACT GGTCAAGCGC 
    AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTACAGC ACACCCCCAC CGATGATGAC 
    ATCTCCAATC TGCTGAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
    GTGGAGGAAC TGCACATGCA ACTGCTTTCC AATGCGAAGG TGCCCATTGA CACATCGCAC 
    TTCTTCTGGC TGGTCACCTA CTTCCTGAAG TTTGCCGCCC AACTGGAGCT GGATATGGAG 
    CACATCGACA CTATTCTCAC CTACGATGTT TTGAGCTACT TGACCTATGA GGGTGTGTCC 
    CTATGTGAGC AACTGGAACT GAATGCCCGA CAGGAGGGCA GTGACCTGAA GCCCTATCTA 
    AGGCGAATGC ACTTGGTGGT GACGGCCATC CGGGAGTTCC TCCAGGCCAT TGATACGTAC 
    AACAAAGTGA CTCATCTGAA CGAGGACGAC AAAGCCCATT TGAGGCAGCT TCAGCTGCAG 
    ATTAGCGAAA TGTCCGATCT GAGGTGCCTT TTTGTGCTTC TGCTGAGGCG TTTCAATCCC 
    AGCATTCATT CCAAGCAGTA TCTTCAGGAT CTGGTGGTTA CCAATCACAT CCTCCTACTC 
    ATTCTGGACA GTTCGGCCAA ACTTGGTGGA TGTCAAACCA TTCGCCTGTC GGAGCACATA 
    ACACAGTTTG CCACGCTGGA GGTGATGCAC TACTATGGCA TTCTGTTGGA GGACTTCAAC 
    AACAACGGAG AGTTTGTCAA TGACTGCATC TTCACCATGA TGCATCACAT CGGTGGCGAT 
    CTGGGCCAGA TTGGGGTTCT ATTTCAACCA ATTATTTTGA AAACCTATTC AAGAATTTGG 
    GAAGCGGACT ATGAACTGTG CGATGACTGG TCTGATCTTA TCGAGTATGT GATTCACAAG 
    TTCATGAATA CTCCTCCGAA GTCGCCACTC ACCATTCCTA CAACTTCCTT GACGGAAATG 
    ACCAAGGAAC ACAACCAGGA GCATACCGTT TGCTCTTGGT CGCAGGAGGA AATGGACACA 
    CTTTATTGGT ATTATGTGCA GAGCAAGAAG AACAACGATA TTGTGGGAAA GATAGTTAAG 
    CTCTTCAGCA ACAACGGCAA CAAGCTGAAA ACCAGGATTT CTATTATCCA ACAACTTTTG 
    CAACAAGACA TTATCACCCT GTTGGAATAC GATGACCTGA TGAAGTTCGA GGATGCGGAG 
    TATCAGAGAA CTTTGCTGAC AACTCCCACT TCCGCAACAA CAGAGTCTGG AATTGAGATT 
    AAGGAGTGCG CCTACGGCAA ACCCTCAGAT GATGTTCAGA TCCTGCTGGA CCTGATCATT 
    AAGGAAAACA AGGCGCAGCA TTTGTTATGG CTGCAAAGGA TCCTCATTGA GTGCTGCTTC 
    GTTAAACTGA CCCTGCGGAG TGGTCTCAAG GTTCCGGAAG GCGATCACAT CATGGAGCCG 
    GTGGCCTACC ACTGCATCTG CAAGCAGAAG TCCATTCCGG TGGTGCAGTG GAACAACGAG 
    CAATCCACTA CGATGCTGTA CCAGCCTTTT GTTCTCCTGC TCCACAAGCT GGGCATTCAG 
    CTGCCGGCGG ACGCGGGCTC GATCTTCGCC AGAATTCCGG ACTACTGGAC ACCGGAGACA 
    ATGTACGGAC TCGCCAAAAA GCTGGGACCG CTGGACAAAC TCAACCTCAA GTTCGACGCC 
    AGTGAACTGG AGGATGCGAC GGCGTCGAGT CCGTCGCGTT ACCACCACAC CGGACCCCGC 
    AACTCGCTCA GCTCGGTAAG CAGCCTGGAC GTGGATCTCG GCGATACCGA GGAGCTGGCC 
    CTTATACCCG AGGTGGATGC GGCCGTGGAG AAGGCACACG CCATGGCATC CACGCCATCG 
    CCCAGCGAGA TTTTCGCGGT TCCCAAGACG AAGCACTGCA ACTCGATCAT CAGATACACA 
    CCAGATCCCA CGCCTCCAGT GCCCAACTGG CTGCAGTTGG TCATGCGCAG CAAATGCAAT 
    CATCGCACAG GTCCGTCTGG TGATCCCAGC GATTGCATTG GCTCCTCGTC GACAACCGTG 
    GACGATGAGG GATTTGGCAA GTCCATCAGT GCAGCCACTT CGCAGGCGGC GAGCACCTCC 
    ATGAGCACGG TTAATCCCAC AACCACTTTG AGCCTGAACA TGCTAAACAC CTTCATGGGA 
    AGCCACAACG AGAACAGCAG CAGTTCTGGT TGCGGGGGCA CCGTCTCCTC CCTGTCCATG 
    GTGGCTCTGA TGAGCACCGG CGCGGCAGGC GGAGGAGGTA ACACCTCCGG GCTGGAAATG 
    GATGTGGACG CCTCCATGAA GTCCTCGTTC GAGCGGCTGG AGGTAAACGG ATCGCACTTC 
    TCGCGGGCCA ACAACTTGGA CCAGGAGTAC AGTGCCATGG TGGCATCTGT GTACGAAAAG 
    GAGAAGGAAT TAAACAGCGA CAATGTCTCT TTGGCCTCGG ACCTGACCAG AATGTATGTG 
    AGCGATGAGG ACGATCGACT TGAGCGAACC GAGATCCGGG TGCCCCACTA TCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_722912.3
    CDS675..4871
    Translation

    Target ORF information:

    RefSeq Version NM_164540.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster timeless (tim), transcript variant P, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_164540.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
    CAAAAACTAC TTAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
    AGTAATACCT CGCCCCCCAA ACAGGGCAGT GGCGATTCCA GCCCCATGCT GACCTCTGAT 
    CCTACCTCTG ATTCCTCGGA CAATGGCAGC AATGGCCGTG GCATGGGCGG TGGCATGCGG 
    GAAGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAGG GTCAGGAGTA TCAGAACGCC 
    ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
    AACGACAGCG AGCAGCCTGG ATCGCCGGAG CAATCGCAGC CCGCCGGCGA GTCCATGGAC 
    GATGGAGATT ACGAGGACCA GAGACACAGG CAACTGAACG AGCATGGCGA GGAGGATGAA 
    GATGAGGACG AAGTGGAGGA GGAAGAGTAC CTACAATTGG GCCCAGCCTC GGAGCCCCTA 
    AACTTAACAC AACAACCAGC TGACAAGGTC AACAACACTA CCAACCCAAC GTCCAGTGCG 
    CCACAAGGCT GCCTGGGCAA TGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
    TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
    TCTGCGGTCA AATTGGGCCA AAAGTCCCCA CATGCCGGCC AGCTCCAGCT GACCAAGGGC 
    AAGTGTTGCC CACAGAAGCG GGAATGCCCC TCCTCGCAGT CGGAGCTATC GGATTGCGGT 
    TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGATGATGGG 
    CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
    CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTTAGAC GCAAAAAACT GGTCAAGCGC 
    AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTACAGC ACACCCCCAC CGATGATGAC 
    ATCTCCAATC TGCTGAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
    GTGGAGGAAC TGCACATGCA ACTGCTTTCC AATGCGAAGG TGCCCATTGA CACATCGCAC 
    TTCTTCTGGC TGGTCACCTA CTTCCTGAAG TTTGCCGCCC AACTGGAGCT GGATATGGAG 
    CACATCGACA CTATTCTCAC CTACGATGTT TTGAGCTACT TGACCTATGA GGGTGTGTCC 
    CTATGTGAGC AACTGGAACT GAATGCCCGA CAGGAGGGCA GTGACCTGAA GCCCTATCTA 
    AGGCGAATGC ACTTGGTGGT GACGGCCATC CGGGAGTTCC TCCAGGCCAT TGATACGTAC 
    AACAAAGTGA CTCATCTGAA CGAGGACGAC AAAGCCCATT TGAGGCAGCT TCAGCTGCAG 
    ATTAGCGAAA TGTCCGATCT GAGGTGCCTT TTTGTGCTTC TGCTGAGGCG TTTCAATCCC 
    AGCATTCATT CCAAGCAGTA TCTTCAGGAT CTGGTGGTTA CCAATCACAT CCTCCTACTC 
    ATTCTGGACA GTTCGGCCAA ACTTGGTGGA TGTCAAACCA TTCGCCTGTC GGAGCACATA 
    ACACAGTTTG CCACGCTGGA GGTGATGCAC TACTATGGCA TTCTGTTGGA GGACTTCAAC 
    AACAACGGAG AGTTTGTCAA TGACTGCATC TTCACCATGA TGCATCACAT CGGTGGCGAT 
    CTGGGCCAGA TTGGGGTTCT ATTTCAACCA ATTATTTTGA AAACCTATTC AAGAATTTGG 
    GAAGCGGACT ATGAACTGTG CGATGACTGG TCTGATCTTA TCGAGTATGT GATTCACAAG 
    TTCATGAATA CTCCTCCGAA GTCGCCACTC ACCATTCCTA CAACTTCCTT GACGGAAATG 
    ACCAAGGAAC ACAACCAGGA GCATACCGTT TGCTCTTGGT CGCAGGAGGA AATGGACACA 
    CTTTATTGGT ATTATGTGCA GAGCAAGAAG AACAACGATA TTGTGGGAAA GATAGTTAAG 
    CTCTTCAGCA ACAACGGCAA CAAGCTGAAA ACCAGGATTT CTATTATCCA ACAACTTTTG 
    CAACAAGACA TTATCACCCT GTTGGAATAC GATGACCTGA TGAAGTTCGA GGATGCGGAG 
    TATCAGAGAA CTTTGCTGAC AACTCCCACT TCCGCAACAA CAGAGTCTGG AATTGAGATT 
    AAGGAGTGCG CCTACGGCAA ACCCTCAGAT GATGTTCAGA TCCTGCTGGA CCTGATCATT 
    AAGGAAAACA AGGCGCAGCA TTTGTTATGG CTGCAAAGGA TCCTCATTGA GTGCTGCTTC 
    GTTAAACTGA CCCTGCGGAG TGGTCTCAAG GTTCCGGAAG GCGATCACAT CATGGAGCCG 
    GTGGCCTACC ACTGCATCTG CAAGCAGAAG TCCATTCCGG TGGTGCAGTG GAACAACGAG 
    CAATCCACTA CGATGCTGTA CCAGCCTTTT GTTCTCCTGC TCCACAAGCT GGGCATTCAG 
    CTGCCGGCGG ACGCGGGCTC GATCTTCGCC AGAATTCCGG ACTACTGGAC ACCGGAGACA 
    ATGTACGGAC TCGCCAAAAA GCTGGGACCG CTGGACAAAC TCAACCTCAA GTTCGACGCC 
    AGTGAACTGG AGGATGCGAC GGCGTCGAGT CCGTCGCGTT ACCACCACAC CGGACCCCGC 
    AACTCGCTCA GCTCGGTAAG CAGCCTGGAC GTGGATCTCG GCGATACCGA GGAGCTGGCC 
    CTTATACCCG AGGTGGATGC GGCCGTGGAG AAGGCACACG CCATGGCATC CACGCCATCG 
    CCCAGCGAGA TTTTCGCGGT TCCCAAGACG AAGCACTGCA ACTCGATCAT CAGATACACA 
    CCAGATCCCA CGCCTCCAGT GCCCAACTGG CTGCAGTTGG TCATGCGCAG CAAATGCAAT 
    CATCGCACAG GTCCGTCTGG TGATCCCAGC GATTGCATTG GCTCCTCGTC GACAACCGTG 
    GACGATGAGG GATTTGGCAA GTCCATCAGT GCAGCCACTT CGCAGGCGGC GAGCACCTCC 
    ATGAGCACGG TTAATCCCAC AACCACTTTG AGCCTGAACA TGCTAAACAC CTTCATGGGA 
    AGCCACAACG AGAACAGCAG CAGTTCTGGT TGCGGGGGCA CCGTCTCCTC CCTGTCCATG 
    GTGGCTCTGA TGAGCACCGG CGCGGCAGGC GGAGGAGGTA ACACCTCCGG GCTGGAAATG 
    GATGTGGACG CCTCCATGAA GTCCTCGTTC GAGCGGCTGG AGGTAAACGG ATCGCACTTC 
    TCGCGGGCCA ACAACTTGGA CCAGGAGTAC AGTGCCATGG TGGCATCTGT GTACGAAAAG 
    GAGAAGGAAT TAAACAGCGA CAATGTCTCT TTGGCCTCGG ACCTGACCAG AATGTATGTG 
    AGCGATGAGG ACGATCGACT TGAGCGAACC GAGATCCGGG TGCCCCACTA TCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    tim ( NM_164541.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722913.3 Drosophila melanogaster timeless (tim), transcript variant L, mRNA.
    Price & Availability↑
    CloneID OFa13654
    Clone ID Related Accession (Same CDS sequence) NM_164541.6
    Accession Version NM_164541.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4194bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product timeless, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_164541.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
    CAAAAACTAC TTAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
    AGTAATACCT CGCCCCCCAA ACAGGGCAGT GGCGATTCCA GCCCCATGCT GACCTCTGAT 
    CCTACCTCTG ATTCCTCGGA CAATGGCAGC AATGGCCGTG GCATGGGCGG TGGCATGCGG 
    GAAGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAGG GTCAGGAGTA TCAGAACGCC 
    ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
    AACGACAGCG AGCAGCCTGG ATCGCCGGAG CAATCGCAGC CCGCCGGCGA GTCCATGGAC 
    GATGGAGATT ACGAGGACCA GAGACACAGG CAACTGAACG AGCATGGCGA GGAGGATGAA 
    GATGAGGACG AAGTGGAGGA GGAAGAGTAC CTACAATTGG GCCCAGCCTC GGAGCCCCTA 
    AACTTAACAC AACAACCAGC TGACAAGGTC AACAACACTA CCAACCCAAC GTCCAGTGCG 
    CCACAAGGCT GCCTGGGCAA TGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
    TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
    TCTGCGGTCA AATTGGGCCA AAAGTCCCCA CATGCCGGCC AGCTCCAGCT GACCAAGGGC 
    AAGTGTTGCC CACAGAAGCG GGAATGCCCC TCCTCGCAGT CGGAGCTATC GGATTGCGGT 
    TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGATGATGGG 
    CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
    CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTTAGAC GCAAAAAACT GGTCAAGCGC 
    AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTACAGC ACACCCCCAC CGATGATGAC 
    ATCTCCAATC TGCTGAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
    GTGGAGGAAC TGCACATGCA ACTGCTTTCC AATGCGGTGC CCATTGACAC ATCGCACTTC 
    TTCTGGCTGG TCACCTACTT CCTGAAGTTT GCCGCCCAAC TGGAGCTGGA TATGGAGCAC 
    ATCGACACTA TTCTCACCTA CGATGTTTTG AGCTACTTGA CCTATGAGGG TGTGTCCCTA 
    TGTGAGCAAC TGGAACTGAA TGCCCGACAG GAGGGCAGTG ACCTGAAGCC CTATCTAAGG 
    CGAATGCACT TGGTGGTGAC GGCCATCCGG GAGTTCCTCC AGGCCATTGA TACGTACAAC 
    AAAGTGACTC ATCTGAACGA GGACGACAAA GCCCATTTGA GGCAGCTTCA GCTGCAGATT 
    AGCGAAATGT CCGATCTGAG GTGCCTTTTT GTGCTTCTGC TGAGGCGTTT CAATCCCAGC 
    ATTCATTCCA AGCAGTATCT TCAGGATCTG GTGGTTACCA ATCACATCCT CCTACTCATT 
    CTGGACAGTT CGGCCAAACT TGGTGGATGT CAAACCATTC GCCTGTCGGA GCACATAACA 
    CAGTTTGCCA CGCTGGAGGT GATGCACTAC TATGGCATTC TGTTGGAGGA CTTCAACAAC 
    AACGGAGAGT TTGTCAATGA CTGCATCTTC ACCATGATGC ATCACATCGG TGGCGATCTG 
    GGCCAGATTG GGGTTCTATT TCAACCAATT ATTTTGAAAA CCTATTCAAG AATTTGGGAA 
    GCGGACTATG AACTGTGCGA TGACTGGTCT GATCTTATCG AGTATGTGAT TCACAAGTTC 
    ATGAATACTC CTCCGAAGTC GCCACTCACC ATTCCTACAA CTTCCTTGAC GGAAATGACC 
    AAGGAACACA ACCAGGAGCA TACCGTTTGC TCTTGGTCGC AGGAGGAAAT GGACACACTT 
    TATTGGTATT ATGTGCAGAG CAAGAAGAAC AACGATATTG TGGGAAAGAT AGTTAAGCTC 
    TTCAGCAACA ACGGCAACAA GCTGAAAACC AGGATTTCTA TTATCCAACA ACTTTTGCAA 
    CAAGACATTA TCACCCTGTT GGAATACGAT GACCTGATGA AGTTCGAGGA TGCGGAGTAT 
    CAGAGAACTT TGCTGACAAC TCCCACTTCC GCAACAACAG AGTCTGGAAT TGAGATTAAG 
    GAGTGCGCCT ACGGCAAACC CTCAGATGAT GTTCAGATCC TGCTGGACCT GATCATTAAG 
    GAAAACAAGG CGCAGCATTT GTTATGGCTG CAAAGGATCC TCATTGAGTG CTGCTTCGTT 
    AAACTGACCC TGCGGAGTGG TCTCAAGGTT CCGGAAGGCG ATCACATCAT GGAGCCGGTG 
    GCCTACCACT GCATCTGCAA GCAGAAGTCC ATTCCGGTGG TGCAGTGGAA CAACGAGCAA 
    TCCACTACGA TGCTGTACCA GCCTTTTGTT CTCCTGCTCC ACAAGCTGGG CATTCAGCTG 
    CCGGCGGACG CGGGCTCGAT CTTCGCCAGA ATTCCGGACT ACTGGACACC GGAGACAATG 
    TACGGACTCG CCAAAAAGCT GGGACCGCTG GACAAACTCA ACCTCAAGTT CGACGCCAGT 
    GAACTGGAGG ATGCGACGGC GTCGAGTCCG TCGCGTTACC ACCACACCGG ACCCCGCAAC 
    TCGCTCAGCT CGGTAAGCAG CCTGGACGTG GATCTCGGCG ATACCGAGGA GCTGGCCCTT 
    ATACCCGAGG TGGATGCGGC CGTGGAGAAG GCACACGCCA TGGCATCCAC GCCATCGCCC 
    AGCGAGATTT TCGCGGTTCC CAAGACGAAG CACTGCAACT CGATCATCAG ATACACACCA 
    GATCCCACGC CTCCAGTGCC CAACTGGCTG CAGTTGGTCA TGCGCAGCAA ATGCAATCAT 
    CGCACAGGTC CGTCTGGTGA TCCCAGCGAT TGCATTGGCT CCTCGTCGAC AACCGTGGAC 
    GATGAGGGAT TTGGCAAGTC CATCAGTGCA GCCACTTCGC AGGCGGCGAG CACCTCCATG 
    AGCACGGTTA ATCCCACAAC CACTTTGAGC CTGAACATGC TAAACACCTT CATGGGAAGC 
    CACAACGAGA ACAGCAGCAG TTCTGGTTGC GGGGGCACCG TCTCCTCCCT GTCCATGGTG 
    GCTCTGATGA GCACCGGCGC GGCAGGCGGA GGAGGTAACA CCTCCGGGCT GGAAATGGAT 
    GTGGACGCCT CCATGAAGTC CTCGTTCGAG CGGCTGGAGG TAAACGGATC GCACTTCTCG 
    CGGGCCAACA ACTTGGACCA GGAGTACAGT GCCATGGTGG CATCTGTGTA CGAAAAGGAG 
    AAGGAATTAA ACAGCGACAA TGTCTCTTTG GCCTCGGACC TGACCAGAAT GTATGTGAGC 
    GATGAGGACG ATCGACTTGA GCGAACCGAG ATCCGGGTGC CCCACTATCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_722913.3
    CDS351..4544
    Translation

    Target ORF information:

    RefSeq Version NM_164541.6
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster timeless (tim), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_164541.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACTGGT TACTAGCAAC TCCGCAGTTG TACAGCGCGT TCTCCTCCTT GGGTTGCTTG 
    GAGGGCGATA CCTATGTGGT CAACCCGAAT GCATTGGCCA TTCTGGAGGA GATCAACTAC 
    AAGCTCACCT ATGAGGACCA AACACTGCGC ACCTTTCGAC GGGCCATTGG ATTTGGCCAG 
    AATGTGAGGT CAGACCTGAT ACCGCTGCTG GAGAATGCCA AGGATGATGC GGTCCTGGAG 
    TCGGTCATCC GGATACTCGT CAATCTGACG GTGCCGGTGG AGTGCCTCTT CTCCGTGGAC 
    GTGATGTACC GCACGGATGT GGGTCGCCAC ACCATCTTCG AGCTGAACAA GCTGCTGTAC 
    ACCAGCAAGG AAGCATTTAC CGAGGCCAGG AGCACCAAGA GCGTGGTGGA GTACATGAAA 
    CACATACTGG AGTCGGACCC TAAGCTGTCG CCGCACAAAT GCGATCAAAT CAACAACTGT 
    CTGCTGCTGC TGAGAAATAT CCTGCACATT CCAGAGACGC ATGCCCATTG CGTGATGCCC 
    ATGATGCAGT CGATGCCGCA TGGCATCTCC ATGCAGAACA CGATCTTGTG GAATCTCTTC 
    ATCCAGAGCA TCGACAAGTT ACTCCTGTAT CTGATGACCT GTCCGCAGAG AGCCTTCTGG 
    GGAGTGACCA TGGTGCAACT GATTGCTTTG ATCTACAAGG ATCAGCATGT GAGCACTCTG 
    CAAAAACTAC TTAGCCTCTG GTTCGAAGCC TCTCTGTCGG AGAGCTCTGA GGATAATGAG 
    AGTAATACCT CGCCCCCCAA ACAGGGCAGT GGCGATTCCA GCCCCATGCT GACCTCTGAT 
    CCTACCTCTG ATTCCTCGGA CAATGGCAGC AATGGCCGTG GCATGGGCGG TGGCATGCGG 
    GAAGGAACAG CGGCCACTTT GCAGGAGGTG AGCCGCAAGG GTCAGGAGTA TCAGAACGCC 
    ATGGCCAGAG TGCCAGCGGA TAAGCCCGAT GGCTCCGAAG AGGCCAGCGA TATGACGGGG 
    AACGACAGCG AGCAGCCTGG ATCGCCGGAG CAATCGCAGC CCGCCGGCGA GTCCATGGAC 
    GATGGAGATT ACGAGGACCA GAGACACAGG CAACTGAACG AGCATGGCGA GGAGGATGAA 
    GATGAGGACG AAGTGGAGGA GGAAGAGTAC CTACAATTGG GCCCAGCCTC GGAGCCCCTA 
    AACTTAACAC AACAACCAGC TGACAAGGTC AACAACACTA CCAACCCAAC GTCCAGTGCG 
    CCACAAGGCT GCCTGGGCAA TGAGCCATTC AAGCCACCAC CTCCTCTGCC AGTCAGAGCC 
    TCCACCTCGG CACACGCTCA AATGCAGAAG TTCAACGAAT CGTCCTACGC GTCCCACGTA 
    TCTGCGGTCA AATTGGGCCA AAAGTCCCCA CATGCCGGCC AGCTCCAGCT GACCAAGGGC 
    AAGTGTTGCC CACAGAAGCG GGAATGCCCC TCCTCGCAGT CGGAGCTATC GGATTGCGGT 
    TATGGCACCC AGGTGGAAAA TCAGGAATCC ATTTCCACCT CCAGCAACGA CGATGATGGG 
    CCGCAGGGCA AGCCGCAGCA CCAGAAGCCT CCGTGTAACA CGAAGCCACG GAATAAACCA 
    CGGACGATTA TGTCGCCAAT GGACAAAAAG GAGCTTAGAC GCAAAAAACT GGTCAAGCGC 
    AGCAAAAGCA GCCTCATCAA CATGAAGGGT CTGGTACAGC ACACCCCCAC CGATGATGAC 
    ATCTCCAATC TGCTGAAGGA ATTCACCGTG GATTTCCTCC TCAAGGGTTA CAGCTATCTG 
    GTGGAGGAAC TGCACATGCA ACTGCTTTCC AATGCGGTGC CCATTGACAC ATCGCACTTC 
    TTCTGGCTGG TCACCTACTT CCTGAAGTTT GCCGCCCAAC TGGAGCTGGA TATGGAGCAC 
    ATCGACACTA TTCTCACCTA CGATGTTTTG AGCTACTTGA CCTATGAGGG TGTGTCCCTA 
    TGTGAGCAAC TGGAACTGAA TGCCCGACAG GAGGGCAGTG ACCTGAAGCC CTATCTAAGG 
    CGAATGCACT TGGTGGTGAC GGCCATCCGG GAGTTCCTCC AGGCCATTGA TACGTACAAC 
    AAAGTGACTC ATCTGAACGA GGACGACAAA GCCCATTTGA GGCAGCTTCA GCTGCAGATT 
    AGCGAAATGT CCGATCTGAG GTGCCTTTTT GTGCTTCTGC TGAGGCGTTT CAATCCCAGC 
    ATTCATTCCA AGCAGTATCT TCAGGATCTG GTGGTTACCA ATCACATCCT CCTACTCATT 
    CTGGACAGTT CGGCCAAACT TGGTGGATGT CAAACCATTC GCCTGTCGGA GCACATAACA 
    CAGTTTGCCA CGCTGGAGGT GATGCACTAC TATGGCATTC TGTTGGAGGA CTTCAACAAC 
    AACGGAGAGT TTGTCAATGA CTGCATCTTC ACCATGATGC ATCACATCGG TGGCGATCTG 
    GGCCAGATTG GGGTTCTATT TCAACCAATT ATTTTGAAAA CCTATTCAAG AATTTGGGAA 
    GCGGACTATG AACTGTGCGA TGACTGGTCT GATCTTATCG AGTATGTGAT TCACAAGTTC 
    ATGAATACTC CTCCGAAGTC GCCACTCACC ATTCCTACAA CTTCCTTGAC GGAAATGACC 
    AAGGAACACA ACCAGGAGCA TACCGTTTGC TCTTGGTCGC AGGAGGAAAT GGACACACTT 
    TATTGGTATT ATGTGCAGAG CAAGAAGAAC AACGATATTG TGGGAAAGAT AGTTAAGCTC 
    TTCAGCAACA ACGGCAACAA GCTGAAAACC AGGATTTCTA TTATCCAACA ACTTTTGCAA 
    CAAGACATTA TCACCCTGTT GGAATACGAT GACCTGATGA AGTTCGAGGA TGCGGAGTAT 
    CAGAGAACTT TGCTGACAAC TCCCACTTCC GCAACAACAG AGTCTGGAAT TGAGATTAAG 
    GAGTGCGCCT ACGGCAAACC CTCAGATGAT GTTCAGATCC TGCTGGACCT GATCATTAAG 
    GAAAACAAGG CGCAGCATTT GTTATGGCTG CAAAGGATCC TCATTGAGTG CTGCTTCGTT 
    AAACTGACCC TGCGGAGTGG TCTCAAGGTT CCGGAAGGCG ATCACATCAT GGAGCCGGTG 
    GCCTACCACT GCATCTGCAA GCAGAAGTCC ATTCCGGTGG TGCAGTGGAA CAACGAGCAA 
    TCCACTACGA TGCTGTACCA GCCTTTTGTT CTCCTGCTCC ACAAGCTGGG CATTCAGCTG 
    CCGGCGGACG CGGGCTCGAT CTTCGCCAGA ATTCCGGACT ACTGGACACC GGAGACAATG 
    TACGGACTCG CCAAAAAGCT GGGACCGCTG GACAAACTCA ACCTCAAGTT CGACGCCAGT 
    GAACTGGAGG ATGCGACGGC GTCGAGTCCG TCGCGTTACC ACCACACCGG ACCCCGCAAC 
    TCGCTCAGCT CGGTAAGCAG CCTGGACGTG GATCTCGGCG ATACCGAGGA GCTGGCCCTT 
    ATACCCGAGG TGGATGCGGC CGTGGAGAAG GCACACGCCA TGGCATCCAC GCCATCGCCC 
    AGCGAGATTT TCGCGGTTCC CAAGACGAAG CACTGCAACT CGATCATCAG ATACACACCA 
    GATCCCACGC CTCCAGTGCC CAACTGGCTG CAGTTGGTCA TGCGCAGCAA ATGCAATCAT 
    CGCACAGGTC CGTCTGGTGA TCCCAGCGAT TGCATTGGCT CCTCGTCGAC AACCGTGGAC 
    GATGAGGGAT TTGGCAAGTC CATCAGTGCA GCCACTTCGC AGGCGGCGAG CACCTCCATG 
    AGCACGGTTA ATCCCACAAC CACTTTGAGC CTGAACATGC TAAACACCTT CATGGGAAGC 
    CACAACGAGA ACAGCAGCAG TTCTGGTTGC GGGGGCACCG TCTCCTCCCT GTCCATGGTG 
    GCTCTGATGA GCACCGGCGC GGCAGGCGGA GGAGGTAACA CCTCCGGGCT GGAAATGGAT 
    GTGGACGCCT CCATGAAGTC CTCGTTCGAG CGGCTGGAGG TAAACGGATC GCACTTCTCG 
    CGGGCCAACA ACTTGGACCA GGAGTACAGT GCCATGGTGG CATCTGTGTA CGAAAAGGAG 
    AAGGAATTAA ACAGCGACAA TGTCTCTTTG GCCTCGGACC TGACCAGAAT GTATGTGAGC 
    GATGAGGACG ATCGACTTGA GCGAACCGAG ATCCGGGTGC CCCACTATCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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