AGO3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGO3
Entrez Gene ID	3355150
Full Name	Argonaute 3
Synonyms	Ago-3,Ago3,BEST:LD15785,CG40300,Dmel\CG40300,ago3,dAgo3
General protein information	Preferred Names Argonaute 3 Names CG40300 gene product from transcript CG40300-RE AGO3-PD AGO3-PE AGO3-PF AGO3-PG Argonaute-3 Argonaute3 BEST:LD15785 CG40300-PD CG40300-PE CG40300-PF CG40300-PG argonaute 3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 80F9-80F9

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043164.3	NP_001036629.2	argonaute 3, isoform E
NM_001043163.3	NP_001036628.2	argonaute 3, isoform F
NM_001043162.3	NP_001036627.2	argonaute 3, isoform D
NM_001170027.2	NP_001163498.1	argonaute 3, isoform G

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Piwil2	NP_067283.1
Drosophila melanogaster (fruit fly)	AGO3	NP_001036629.2
Pan troglodytes (chimpanzee)	PIWIL2	XP_003311683.1
Canis lupus familiaris (dog)	PIWIL2	XP_543251.2
Rattus norvegicus (Norway rat)	Piwil2	NP_001100746.1
Danio rerio (zebrafish)	piwil2	NP_001073668.2
Xenopus tropicalis (tropical clawed frog)	piwil2	NP_001106470.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008862	XP_319604.4
Homo sapiens (human)	PIWIL2	NP_001129193.1
Bos taurus (cattle)	PIWIL2	XP_002689814.1
	LOC708028	XP_002808520.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that Cbp80 knockdown not only led to the upregulation of transposons, but also to delocalization of Piwi, Aub and Ago3, key factors in the piRNA biosynthesis pathway.
Title: Cbp80 is needed for the expression of piRNA components and piRNAs.

The lack of Spn-E caused a significant drop of protein levels of key ping-pong participants, Aubergine (Aub) and AGO3 proteins of PIWI subfamily, in the germline of both males and females, but did not disrupt of their assembly in nuage granules.
Title: RNA helicase Spn-E is required to maintain Aub and AGO3 protein levels for piRNA silencing in the germline of Drosophila.

We find there are large differences in evolutionary rates and gene turnover between pathways, and that paralogs of Ago2, Ago3, and Piwi/Aub show contrasting rates of evolution after duplication.
Title: Duplication and Diversification of Dipteran Argonaute Genes, and the Evolutionary Divergence of Piwi and Aubergine.

Although Argonaute3 slicing is required to efficiently trigger phased piRNA production, an alternative, slicing-independent pathway suffices to generate Piwi-bound piRNAs that repress transcription of a subset of transposon families.
Title: Slicing and Binding by Ago3 or Aub Trigger Piwi-Bound piRNA Production by Distinct Mechanisms.

Krimper interacts directly with Aub and Ago3 to coordinate the assembly of the ping-pong piRNA processing complex.
Title: Aub and Ago3 Are Recruited to Nuage through Two Mechanisms to Form a Ping-Pong Complex Assembled by Krimper.

The following AGO3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGO3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19646	NM_001170027.2 Latest version!	Drosophila melanogaster argonaute 3, transcript variant G (AGO3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa20047	NM_001043162.3 Latest version!	Drosophila melanogaster argonaute 3, transcript variant D (AGO3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa20047	NM_001043163.3 Latest version!	Drosophila melanogaster argonaute 3, transcript variant F (AGO3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa20047	NM_001043164.3 Latest version!	Drosophila melanogaster argonaute 3, transcript variant E (AGO3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGO3 ( NM_001170027.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163498.1 Drosophila melanogaster argonaute 3, transcript variant G (AGO3), partial mRNA.

Price & Availability↑

CloneID	OFa19646
Clone ID Related Accession (Same CDS sequence)	NM_001170027.2
Accession Version	NM_001170027.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	argonaute 3, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001170027.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 3' end.

ATGTCTGGAA GAGGAAATTT GTTGAGCTTA TTTAATAAAA ATGCTGGCAA TATGGGAAAA 
TCAATTTCAA GTAAAGATCA TGAAATCGAC AGTGGCTTGG ATTTTAACAA CTCTGAATCA 
AGTGGAGAAC GTTTACTGTC AAGCCATAAT ATTGAAACTG ATCTTATAAC CACTCTTCAA 
CATGTAAACA TCTCGGTCGG CCGAGGACGT GCTAGGCTTA TAGACACATT AAAAACAGAT 
GACCATACAT CAAATCAATT TATAACATCT GAATCAAAGG AAAACATTAC CAAAAAGACA 
AAGGGCCCCG AATCGGAAGC AATTGCTTCA GAAAATGGAT TATTTTTTCC CGATTTAATT 
TACGGATCTA AAGGTTCATC TGTTAATATA TATTGTAATT ACTTAAAGCT AACGACGGAT 
GAATCCAAGG GAGTTTTTAA TTACGAAGTG CGTTTTTTTC CTCCCATTGA CTCTGTTCAT 
TTAAGAATAA AATATTTAAA TGATCATAAG GATAAACTCG GTGGAACCAA GACATTTGAC 
GGAAATACTT TATATTTACC TATTTTGTTA CCAAATAAGA TGACCGTCTT TATTTCAAAG 
GCGGAAGATG TTGAGTTGCA GATTCGCATT TTATACAAAA AAAAAGAGGA AATGAGGAAC 
TGCACGCAAT TATATAATAT TTTATTCGAT AGAGTTATGA AAGTATTGAA TTACGTAAAA 
TTTGATCGCA AGCAGTTTGA TCCTTCGCGT CCAAAAATAA TACCACTGGC AAAGTTAGAA 
GTTTGGCCTG GCTATGTTAC AGCCGTTGAC GAATACAAAG GTGGTTTAAT GCTATGCTGC 
GACGTTTCGC ATCGTATACT TTGCCAAAAA ACTGTTCTAG AAATGCTGGT TGATCTTTAT 
CAGCAAAATG TAGAGCATTA CCAAGAATCA GCAAGAAAAA TGTTGGTTGG AAATATTGTC 
TTAACTAGGT ATAACAATCG TACATATAAA ATAAATGACA TTTGTTTTGA TCAAAATCCA 
ACATGTCAGT TTGAAATTAA AACTGGCTGC ACAAGTTACG TGGAGTATTA CAAACAATAT 
CACAATATAA ACATAAAGGA CGTTAATCAA CCATTGATTT ACAGTATAAA GAAATCAAGA 
GGAATTCCAG CAGAAAGGGA AAATTTACAG TTTTGTTTAA TTCCAGAATT GTGTTATCTT 
ACTGGGCTGC GAGACGAAGT ACGGTCAGAT AACAAGCTTA TGCGCGAGAT TGCTACATTT 
ACAAGAGTTT CGCCAAATCA ACGACAGATG GCGTTAAATA AATTTTACGA AAATGTTTCA 
AATACACCCG CCGCACAAGA AATTCTAAAT AGCTGGGGTC TTTCATTAAC GAATAATTCC 
AACAAGATAA GTGGGCGTCA AATGGACATA GAGCAAATAT ATTTTTCAAA AATTAGTGTA 
TCGGCCGGAA GGTCCGCAGA GTTCTCCAAA CATGCTGTGA CCAATGAAAT GTTGAAAGTA 
GTCCACCTGA GTAAATGGAT CATTATACAT TTAAGAAATT ATAGACAAGC TGCAACTAGC 
TTGCTCGATA ACATGAAACA GGCTTGTGAG TCACTTGGTA TGAACATTTC TAACCCAACA 
ATGATTTCGT TAGATCATGA CCGAATCGAT GCCTACATTC AAGCACTGCG GCGAAATATA 
ACTATGAATA CGCAAATGGT CGTATGTATT TGTCATAACA GAAGAGATGA TCGTTATGCT 
GCAATAAAAA AAATATGTTG CTCGGAAATA CCTATACCAT CACAGGTTAT AAATGCTAAA 
ACTTTACAAA ATGATCTTAA AATTCGATCC GTTGTTCAAA AAATTGTTCT ACAAATGAAC 
TGTAAACTCG GAGGCTCATT ATGGACGGTA AAAATTCCAT TTAAAAATGT AATGATTTGC 
GGAATAGACT CATATCATGA CCCATCTAAT CGGGGGAATT CTGTTGCTGC ATTTGTGGCC 
TCCATAAATT CGTCGTATTC ACAGTGGTAC AGTAAAGCTG TTGTACAAAC AAAACGTGAA 
GAAATTGTTA ATGGGCTCTC TGCATCTTTT GAAATTGCAT TAAAAATGTA TCGTAAAAGG 
AATGGCAAAC TCCCCACAAA TATTATAATA TACAGAGATG GAATTGGAGA TGGACAGCTT 
TATACATGTC TTAATTATGA AATTCCACAA TTTGAAATGG TGTGTGGAAA TCGCATAAAA 
ATTTCATATA TTGTAGTCCA AAAACGAATT AACACTCGCA TCTTCTCGGG TAGCGGCATT 
CATCTAGAAA ACCCATTGCC AGGAACTGTC GTAGATCAAC ATATAACAAA ATCAAATATG 
TATGATTTTT TTTTGGTTTC GCAATTGGTA CGACAGGGTA CTGTAACCCC AACTCATTAT 
GTTGTTCTTC GAGATGACTG CAATTATGGA CCCGACATAA TTCAAAAACT AAGTTACAAA 
CTTTGTTTTT TATATTACAA TTGGGCTGGT ACAGTACGAA TTCCAGCTTG TTGTATGGTG 
AGTCATAATT ACCACTTAAT TTTTTTTAAA TCCACCATGA TAACACAAGA TACCATTAAC 
GACTTTACTA GAGCGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163498.1
CDS	128..2728
Translation	MSGRGNLLSLFNKNAGNMGKSISSKDHEIDSGLDFNNSESSGERLLSSHNIETDLITTLQHVNISVGRGRARLIDTLKTDDHTSNQFITSESKENITKKTKGPESEAIASENGLFFPDLIYGSKGSSVNIYCNYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLNDHKDKLGGTKTFDGNTLYLPILLPNKMTVFISKAEDVELQIRILYKKKEEMRNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRPKIIPLAKLEVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQQNVEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCTSYVEYYKQYHNINIKDVNQPLIYSIKKSRGIPAERENLQFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGRSAEFSKHAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYEIPQFEMVCGNRIKISYIVVQKRINTRIFSGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMVSHNYHLIFFKSTMITQDTINDFTRAL

Target ORF information:

RefSeq Version	NM_001170027.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster argonaute 3, transcript variant G (AGO3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170027.2

ORF Insert Sequence:

ATGTCTGGAA GAGGAAATTT GTTGAGCTTA TTTAATAAAA ATGCTGGCAA TATGGGAAAA 
TCAATTTCAA GTAAAGATCA TGAAATCGAC AGTGGCTTGG ATTTTAACAA CTCTGAATCA 
AGTGGAGAAC GTTTACTGTC AAGCCATAAT ATTGAAACTG ATCTTATAAC CACTCTTCAA 
CATGTAAACA TCTCGGTCGG CCGAGGACGT GCTAGGCTTA TAGACACATT AAAAACAGAT 
GACCATACAT CAAATCAATT TATAACATCT GAATCAAAGG AAAACATTAC CAAAAAGACA 
AAGGGCCCCG AATCGGAAGC AATTGCTTCA GAAAATGGAT TATTTTTTCC CGATTTAATT 
TACGGATCTA AAGGTTCATC TGTTAATATA TATTGTAATT ACTTAAAGCT AACGACGGAT 
GAATCCAAGG GAGTTTTTAA TTACGAAGTG CGTTTTTTTC CTCCCATTGA CTCTGTTCAT 
TTAAGAATAA AATATTTAAA TGATCATAAG GATAAACTCG GTGGAACCAA GACATTTGAC 
GGAAATACTT TATATTTACC TATTTTGTTA CCAAATAAGA TGACCGTCTT TATTTCAAAG 
GCGGAAGATG TTGAGTTGCA GATTCGCATT TTATACAAAA AAAAAGAGGA AATGAGGAAC 
TGCACGCAAT TATATAATAT TTTATTCGAT AGAGTTATGA AAGTATTGAA TTACGTAAAA 
TTTGATCGCA AGCAGTTTGA TCCTTCGCGT CCAAAAATAA TACCACTGGC AAAGTTAGAA 
GTTTGGCCTG GCTATGTTAC AGCCGTTGAC GAATACAAAG GTGGTTTAAT GCTATGCTGC 
GACGTTTCGC ATCGTATACT TTGCCAAAAA ACTGTTCTAG AAATGCTGGT TGATCTTTAT 
CAGCAAAATG TAGAGCATTA CCAAGAATCA GCAAGAAAAA TGTTGGTTGG AAATATTGTC 
TTAACTAGGT ATAACAATCG TACATATAAA ATAAATGACA TTTGTTTTGA TCAAAATCCA 
ACATGTCAGT TTGAAATTAA AACTGGCTGC ACAAGTTACG TGGAGTATTA CAAACAATAT 
CACAATATAA ACATAAAGGA CGTTAATCAA CCATTGATTT ACAGTATAAA GAAATCAAGA 
GGAATTCCAG CAGAAAGGGA AAATTTACAG TTTTGTTTAA TTCCAGAATT GTGTTATCTT 
ACTGGGCTGC GAGACGAAGT ACGGTCAGAT AACAAGCTTA TGCGCGAGAT TGCTACATTT 
ACAAGAGTTT CGCCAAATCA ACGACAGATG GCGTTAAATA AATTTTACGA AAATGTTTCA 
AATACACCCG CCGCACAAGA AATTCTAAAT AGCTGGGGTC TTTCATTAAC GAATAATTCC 
AACAAGATAA GTGGGCGTCA AATGGACATA GAGCAAATAT ATTTTTCAAA AATTAGTGTA 
TCGGCCGGAA GGTCCGCAGA GTTCTCCAAA CATGCTGTGA CCAATGAAAT GTTGAAAGTA 
GTCCACCTGA GTAAATGGAT CATTATACAT TTAAGAAATT ATAGACAAGC TGCAACTAGC 
TTGCTCGATA ACATGAAACA GGCTTGTGAG TCACTTGGTA TGAACATTTC TAACCCAACA 
ATGATTTCGT TAGATCATGA CCGAATCGAT GCCTACATTC AAGCACTGCG GCGAAATATA 
ACTATGAATA CGCAAATGGT CGTATGTATT TGTCATAACA GAAGAGATGA TCGTTATGCT 
GCAATAAAAA AAATATGTTG CTCGGAAATA CCTATACCAT CACAGGTTAT AAATGCTAAA 
ACTTTACAAA ATGATCTTAA AATTCGATCC GTTGTTCAAA AAATTGTTCT ACAAATGAAC 
TGTAAACTCG GAGGCTCATT ATGGACGGTA AAAATTCCAT TTAAAAATGT AATGATTTGC 
GGAATAGACT CATATCATGA CCCATCTAAT CGGGGGAATT CTGTTGCTGC ATTTGTGGCC 
TCCATAAATT CGTCGTATTC ACAGTGGTAC AGTAAAGCTG TTGTACAAAC AAAACGTGAA 
GAAATTGTTA ATGGGCTCTC TGCATCTTTT GAAATTGCAT TAAAAATGTA TCGTAAAAGG 
AATGGCAAAC TCCCCACAAA TATTATAATA TACAGAGATG GAATTGGAGA TGGACAGCTT 
TATACATGTC TTAATTATGA AATTCCACAA TTTGAAATGG TGTGTGGAAA TCGCATAAAA 
ATTTCATATA TTGTAGTCCA AAAACGAATT AACACTCGCA TCTTCTCGGG TAGCGGCATT 
CATCTAGAAA ACCCATTGCC AGGAACTGTC GTAGATCAAC ATATAACAAA ATCAAATATG 
TATGATTTTT TTTTGGTTTC GCAATTGGTA CGACAGGGTA CTGTAACCCC AACTCATTAT 
GTTGTTCTTC GAGATGACTG CAATTATGGA CCCGACATAA TTCAAAAACT AAGTTACAAA 
CTTTGTTTTT TATATTACAA TTGGGCTGGT ACAGTACGAA TTCCAGCTTG TTGTATGGTG 
AGTCATAATT ACCACTTAAT TTTTTTTAAA TCCACCATGA TAACACAAGA TACCATTAAC 
GACTTTACTA GAGCGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO3 ( NM_001043164.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036629.2 Drosophila melanogaster argonaute 3, transcript variant E (AGO3), mRNA.

Price & Availability↑

CloneID	OFa20047
Clone ID Related Accession (Same CDS sequence)	NM_001043164.3 , NM_001043163.3 , NM_001043162.3
Accession Version	NM_001043164.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	argonaute 3, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001043164.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGGAA GAGGAAATTT GTTGAGCTTA TTTAATAAAA ATGCTGGCAA TATGGGAAAA 
TCAATTTCAA GTAAAGATCA TGAAATCGAC AGTGGCTTGG ATTTTAACAA CTCTGAATCA 
AGTGGAGAAC GTTTACTGTC AAGCCATAAT ATTGAAACTG ATCTTATAAC CACTCTTCAA 
CATGTAAACA TCTCGGTCGG CCGAGGACGT GCTAGGCTTA TAGACACATT AAAAACAGAT 
GACCATACAT CAAATCAATT TATAACATCT GAATCAAAGG AAAACATTAC CAAAAAGACA 
AAGGGCCCCG AATCGGAAGC AATTGCTTCA GAAAATGGAT TATTTTTTCC CGATTTAATT 
TACGGATCTA AAGGTTCATC TGTTAATATA TATTGTAATT ACTTAAAGCT AACGACGGAT 
GAATCCAAGG GAGTTTTTAA TTACGAAGTG CGTTTTTTTC CTCCCATTGA CTCTGTTCAT 
TTAAGAATAA AATATTTAAA TGATCATAAG GATAAACTCG GTGGAACCAA GACATTTGAC 
GGAAATACTT TATATTTACC TATTTTGTTA CCAAATAAGA TGACCGTCTT TATTTCAAAG 
GCGGAAGATG TTGAGTTGCA GATTCGCATT TTATACAAAA AAAAAGAGGA AATGAGGAAC 
TGCACGCAAT TATATAATAT TTTATTCGAT AGAGTTATGA AAGTATTGAA TTACGTAAAA 
TTTGATCGCA AGCAGTTTGA TCCTTCGCGT CCAAAAATAA TACCACTGGC AAAGTTAGAA 
GTTTGGCCTG GCTATGTTAC AGCCGTTGAC GAATACAAAG GTGGTTTAAT GCTATGCTGC 
GACGTTTCGC ATCGTATACT TTGCCAAAAA ACTGTTCTAG AAATGCTGGT TGATCTTTAT 
CAGCAAAATG TAGAGCATTA CCAAGAATCA GCAAGAAAAA TGTTGGTTGG AAATATTGTC 
TTAACTAGGT ATAACAATCG TACATATAAA ATAAATGACA TTTGTTTTGA TCAAAATCCA 
ACATGTCAGT TTGAAATTAA AACTGGCTGC ACAAGTTACG TGGAGTATTA CAAACAATAT 
CACAATATAA ACATAAAGGA CGTTAATCAA CCATTGATTT ACAGTATAAA GAAATCAAGA 
GGAATTCCAG CAGAAAGGGA AAATTTACAG TTTTGTTTAA TTCCAGAATT GTGTTATCTT 
ACTGGGCTGC GAGACGAAGT ACGGTCAGAT AACAAGCTTA TGCGCGAGAT TGCTACATTT 
ACAAGAGTTT CGCCAAATCA ACGACAGATG GCGTTAAATA AATTTTACGA AAATGTTTCA 
AATACACCCG CCGCACAAGA AATTCTAAAT AGCTGGGGTC TTTCATTAAC GAATAATTCC 
AACAAGATAA GTGGGCGTCA AATGGACATA GAGCAAATAT ATTTTTCAAA AATTAGTGTA 
TCGGCCGGAA GGTCCGCAGA GTTCTCCAAA CATGCTGTGA CCAATGAAAT GTTGAAAGTA 
GTCCACCTGA GTAAATGGAT CATTATACAT TTAAGAAATT ATAGACAAGC TGCAACTAGC 
TTGCTCGATA ACATGAAACA GGCTTGTGAG TCACTTGGTA TGAACATTTC TAACCCAACA 
ATGATTTCGT TAGATCATGA CCGAATCGAT GCCTACATTC AAGCACTGCG GCGAAATATA 
ACTATGAATA CGCAAATGGT CGTATGTATT TGTCATAACA GAAGAGATGA TCGTTATGCT 
GCAATAAAAA AAATATGTTG CTCGGAAATA CCTATACCAT CACAGGTTAT AAATGCTAAA 
ACTTTACAAA ATGATCTTAA AATTCGATCC GTTGTTCAAA AAATTGTTCT ACAAATGAAC 
TGTAAACTCG GAGGCTCATT ATGGACGGTA AAAATTCCAT TTAAAAATGT AATGATTTGC 
GGAATAGACT CATATCATGA CCCATCTAAT CGGGGGAATT CTGTTGCTGC ATTTGTGGCC 
TCCATAAATT CGTCGTATTC ACAGTGGTAC AGTAAAGCTG TTGTACAAAC AAAACGTGAA 
GAAATTGTTA ATGGGCTCTC TGCATCTTTT GAAATTGCAT TAAAAATGTA TCGTAAAAGG 
AATGGCAAAC TCCCCACAAA TATTATAATA TACAGAGATG GAATTGGAGA TGGACAGCTT 
TATACATGTC TTAATTATGA AATTCCACAA TTTGAAATGG TGTGTGGAAA TCGCATAAAA 
ATTTCATATA TTGTAGTCCA AAAACGAATT AACACTCGCA TCTTCTCGGG TAGCGGCATT 
CATCTAGAAA ACCCATTGCC AGGAACTGTC GTAGATCAAC ATATAACAAA ATCAAATATG 
TATGATTTTT TTTTGGTTTC GCAATTGGTA CGACAGGGTA CTGTAACCCC AACTCATTAT 
GTTGTTCTTC GAGATGACTG CAATTATGGA CCCGACATAA TTCAAAAACT AAGTTACAAA 
CTTTGTTTTT TATATTACAA TTGGGCTGGT ACAGTACGAA TTCCAGCTTG TTGTATGTAC 
GCTCACAAAT TGGCTTATTT GATTGGACAA AGTATTCAAC GAGATGTGGC TGAAGCACTT 
TCTGAAAAAC TATTTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036629.2
CDS	302..2905
Translation	MSGRGNLLSLFNKNAGNMGKSISSKDHEIDSGLDFNNSESSGERLLSSHNIETDLITTLQHVNISVGRGRARLIDTLKTDDHTSNQFITSESKENITKKTKGPESEAIASENGLFFPDLIYGSKGSSVNIYCNYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLNDHKDKLGGTKTFDGNTLYLPILLPNKMTVFISKAEDVELQIRILYKKKEEMRNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRPKIIPLAKLEVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQQNVEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCTSYVEYYKQYHNINIKDVNQPLIYSIKKSRGIPAERENLQFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGRSAEFSKHAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYEIPQFEMVCGNRIKISYIVVQKRINTRIFSGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRDVAEALSEKLFYL

Target ORF information:

RefSeq Version	NM_001043164.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster argonaute 3, transcript variant E (AGO3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043164.3

ORF Insert Sequence:

ATGTCTGGAA GAGGAAATTT GTTGAGCTTA TTTAATAAAA ATGCTGGCAA TATGGGAAAA 
TCAATTTCAA GTAAAGATCA TGAAATCGAC AGTGGCTTGG ATTTTAACAA CTCTGAATCA 
AGTGGAGAAC GTTTACTGTC AAGCCATAAT ATTGAAACTG ATCTTATAAC CACTCTTCAA 
CATGTAAACA TCTCGGTCGG CCGAGGACGT GCTAGGCTTA TAGACACATT AAAAACAGAT 
GACCATACAT CAAATCAATT TATAACATCT GAATCAAAGG AAAACATTAC CAAAAAGACA 
AAGGGCCCCG AATCGGAAGC AATTGCTTCA GAAAATGGAT TATTTTTTCC CGATTTAATT 
TACGGATCTA AAGGTTCATC TGTTAATATA TATTGTAATT ACTTAAAGCT AACGACGGAT 
GAATCCAAGG GAGTTTTTAA TTACGAAGTG CGTTTTTTTC CTCCCATTGA CTCTGTTCAT 
TTAAGAATAA AATATTTAAA TGATCATAAG GATAAACTCG GTGGAACCAA GACATTTGAC 
GGAAATACTT TATATTTACC TATTTTGTTA CCAAATAAGA TGACCGTCTT TATTTCAAAG 
GCGGAAGATG TTGAGTTGCA GATTCGCATT TTATACAAAA AAAAAGAGGA AATGAGGAAC 
TGCACGCAAT TATATAATAT TTTATTCGAT AGAGTTATGA AAGTATTGAA TTACGTAAAA 
TTTGATCGCA AGCAGTTTGA TCCTTCGCGT CCAAAAATAA TACCACTGGC AAAGTTAGAA 
GTTTGGCCTG GCTATGTTAC AGCCGTTGAC GAATACAAAG GTGGTTTAAT GCTATGCTGC 
GACGTTTCGC ATCGTATACT TTGCCAAAAA ACTGTTCTAG AAATGCTGGT TGATCTTTAT 
CAGCAAAATG TAGAGCATTA CCAAGAATCA GCAAGAAAAA TGTTGGTTGG AAATATTGTC 
TTAACTAGGT ATAACAATCG TACATATAAA ATAAATGACA TTTGTTTTGA TCAAAATCCA 
ACATGTCAGT TTGAAATTAA AACTGGCTGC ACAAGTTACG TGGAGTATTA CAAACAATAT 
CACAATATAA ACATAAAGGA CGTTAATCAA CCATTGATTT ACAGTATAAA GAAATCAAGA 
GGAATTCCAG CAGAAAGGGA AAATTTACAG TTTTGTTTAA TTCCAGAATT GTGTTATCTT 
ACTGGGCTGC GAGACGAAGT ACGGTCAGAT AACAAGCTTA TGCGCGAGAT TGCTACATTT 
ACAAGAGTTT CGCCAAATCA ACGACAGATG GCGTTAAATA AATTTTACGA AAATGTTTCA 
AATACACCCG CCGCACAAGA AATTCTAAAT AGCTGGGGTC TTTCATTAAC GAATAATTCC 
AACAAGATAA GTGGGCGTCA AATGGACATA GAGCAAATAT ATTTTTCAAA AATTAGTGTA 
TCGGCCGGAA GGTCCGCAGA GTTCTCCAAA CATGCTGTGA CCAATGAAAT GTTGAAAGTA 
GTCCACCTGA GTAAATGGAT CATTATACAT TTAAGAAATT ATAGACAAGC TGCAACTAGC 
TTGCTCGATA ACATGAAACA GGCTTGTGAG TCACTTGGTA TGAACATTTC TAACCCAACA 
ATGATTTCGT TAGATCATGA CCGAATCGAT GCCTACATTC AAGCACTGCG GCGAAATATA 
ACTATGAATA CGCAAATGGT CGTATGTATT TGTCATAACA GAAGAGATGA TCGTTATGCT 
GCAATAAAAA AAATATGTTG CTCGGAAATA CCTATACCAT CACAGGTTAT AAATGCTAAA 
ACTTTACAAA ATGATCTTAA AATTCGATCC GTTGTTCAAA AAATTGTTCT ACAAATGAAC 
TGTAAACTCG GAGGCTCATT ATGGACGGTA AAAATTCCAT TTAAAAATGT AATGATTTGC 
GGAATAGACT CATATCATGA CCCATCTAAT CGGGGGAATT CTGTTGCTGC ATTTGTGGCC 
TCCATAAATT CGTCGTATTC ACAGTGGTAC AGTAAAGCTG TTGTACAAAC AAAACGTGAA 
GAAATTGTTA ATGGGCTCTC TGCATCTTTT GAAATTGCAT TAAAAATGTA TCGTAAAAGG 
AATGGCAAAC TCCCCACAAA TATTATAATA TACAGAGATG GAATTGGAGA TGGACAGCTT 
TATACATGTC TTAATTATGA AATTCCACAA TTTGAAATGG TGTGTGGAAA TCGCATAAAA 
ATTTCATATA TTGTAGTCCA AAAACGAATT AACACTCGCA TCTTCTCGGG TAGCGGCATT 
CATCTAGAAA ACCCATTGCC AGGAACTGTC GTAGATCAAC ATATAACAAA ATCAAATATG 
TATGATTTTT TTTTGGTTTC GCAATTGGTA CGACAGGGTA CTGTAACCCC AACTCATTAT 
GTTGTTCTTC GAGATGACTG CAATTATGGA CCCGACATAA TTCAAAAACT AAGTTACAAA 
CTTTGTTTTT TATATTACAA TTGGGCTGGT ACAGTACGAA TTCCAGCTTG TTGTATGTAC 
GCTCACAAAT TGGCTTATTT GATTGGACAA AGTATTCAAC GAGATGTGGC TGAAGCACTT 
TCTGAAAAAC TATTTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO3 ( NM_001043163.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036628.2 Drosophila melanogaster argonaute 3, transcript variant F (AGO3), mRNA.

Price & Availability↑

CloneID	OFa20047
Clone ID Related Accession (Same CDS sequence)	NM_001043164.3 , NM_001043163.3 , NM_001043162.3
Accession Version	NM_001043163.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	argonaute 3, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001043163.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGGAA GAGGAAATTT GTTGAGCTTA TTTAATAAAA ATGCTGGCAA TATGGGAAAA 
TCAATTTCAA GTAAAGATCA TGAAATCGAC AGTGGCTTGG ATTTTAACAA CTCTGAATCA 
AGTGGAGAAC GTTTACTGTC AAGCCATAAT ATTGAAACTG ATCTTATAAC CACTCTTCAA 
CATGTAAACA TCTCGGTCGG CCGAGGACGT GCTAGGCTTA TAGACACATT AAAAACAGAT 
GACCATACAT CAAATCAATT TATAACATCT GAATCAAAGG AAAACATTAC CAAAAAGACA 
AAGGGCCCCG AATCGGAAGC AATTGCTTCA GAAAATGGAT TATTTTTTCC CGATTTAATT 
TACGGATCTA AAGGTTCATC TGTTAATATA TATTGTAATT ACTTAAAGCT AACGACGGAT 
GAATCCAAGG GAGTTTTTAA TTACGAAGTG CGTTTTTTTC CTCCCATTGA CTCTGTTCAT 
TTAAGAATAA AATATTTAAA TGATCATAAG GATAAACTCG GTGGAACCAA GACATTTGAC 
GGAAATACTT TATATTTACC TATTTTGTTA CCAAATAAGA TGACCGTCTT TATTTCAAAG 
GCGGAAGATG TTGAGTTGCA GATTCGCATT TTATACAAAA AAAAAGAGGA AATGAGGAAC 
TGCACGCAAT TATATAATAT TTTATTCGAT AGAGTTATGA AAGTATTGAA TTACGTAAAA 
TTTGATCGCA AGCAGTTTGA TCCTTCGCGT CCAAAAATAA TACCACTGGC AAAGTTAGAA 
GTTTGGCCTG GCTATGTTAC AGCCGTTGAC GAATACAAAG GTGGTTTAAT GCTATGCTGC 
GACGTTTCGC ATCGTATACT TTGCCAAAAA ACTGTTCTAG AAATGCTGGT TGATCTTTAT 
CAGCAAAATG TAGAGCATTA CCAAGAATCA GCAAGAAAAA TGTTGGTTGG AAATATTGTC 
TTAACTAGGT ATAACAATCG TACATATAAA ATAAATGACA TTTGTTTTGA TCAAAATCCA 
ACATGTCAGT TTGAAATTAA AACTGGCTGC ACAAGTTACG TGGAGTATTA CAAACAATAT 
CACAATATAA ACATAAAGGA CGTTAATCAA CCATTGATTT ACAGTATAAA GAAATCAAGA 
GGAATTCCAG CAGAAAGGGA AAATTTACAG TTTTGTTTAA TTCCAGAATT GTGTTATCTT 
ACTGGGCTGC GAGACGAAGT ACGGTCAGAT AACAAGCTTA TGCGCGAGAT TGCTACATTT 
ACAAGAGTTT CGCCAAATCA ACGACAGATG GCGTTAAATA AATTTTACGA AAATGTTTCA 
AATACACCCG CCGCACAAGA AATTCTAAAT AGCTGGGGTC TTTCATTAAC GAATAATTCC 
AACAAGATAA GTGGGCGTCA AATGGACATA GAGCAAATAT ATTTTTCAAA AATTAGTGTA 
TCGGCCGGAA GGTCCGCAGA GTTCTCCAAA CATGCTGTGA CCAATGAAAT GTTGAAAGTA 
GTCCACCTGA GTAAATGGAT CATTATACAT TTAAGAAATT ATAGACAAGC TGCAACTAGC 
TTGCTCGATA ACATGAAACA GGCTTGTGAG TCACTTGGTA TGAACATTTC TAACCCAACA 
ATGATTTCGT TAGATCATGA CCGAATCGAT GCCTACATTC AAGCACTGCG GCGAAATATA 
ACTATGAATA CGCAAATGGT CGTATGTATT TGTCATAACA GAAGAGATGA TCGTTATGCT 
GCAATAAAAA AAATATGTTG CTCGGAAATA CCTATACCAT CACAGGTTAT AAATGCTAAA 
ACTTTACAAA ATGATCTTAA AATTCGATCC GTTGTTCAAA AAATTGTTCT ACAAATGAAC 
TGTAAACTCG GAGGCTCATT ATGGACGGTA AAAATTCCAT TTAAAAATGT AATGATTTGC 
GGAATAGACT CATATCATGA CCCATCTAAT CGGGGGAATT CTGTTGCTGC ATTTGTGGCC 
TCCATAAATT CGTCGTATTC ACAGTGGTAC AGTAAAGCTG TTGTACAAAC AAAACGTGAA 
GAAATTGTTA ATGGGCTCTC TGCATCTTTT GAAATTGCAT TAAAAATGTA TCGTAAAAGG 
AATGGCAAAC TCCCCACAAA TATTATAATA TACAGAGATG GAATTGGAGA TGGACAGCTT 
TATACATGTC TTAATTATGA AATTCCACAA TTTGAAATGG TGTGTGGAAA TCGCATAAAA 
ATTTCATATA TTGTAGTCCA AAAACGAATT AACACTCGCA TCTTCTCGGG TAGCGGCATT 
CATCTAGAAA ACCCATTGCC AGGAACTGTC GTAGATCAAC ATATAACAAA ATCAAATATG 
TATGATTTTT TTTTGGTTTC GCAATTGGTA CGACAGGGTA CTGTAACCCC AACTCATTAT 
GTTGTTCTTC GAGATGACTG CAATTATGGA CCCGACATAA TTCAAAAACT AAGTTACAAA 
CTTTGTTTTT TATATTACAA TTGGGCTGGT ACAGTACGAA TTCCAGCTTG TTGTATGTAC 
GCTCACAAAT TGGCTTATTT GATTGGACAA AGTATTCAAC GAGATGTGGC TGAAGCACTT 
TCTGAAAAAC TATTTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036628.2
CDS	101..2704
Translation	MSGRGNLLSLFNKNAGNMGKSISSKDHEIDSGLDFNNSESSGERLLSSHNIETDLITTLQHVNISVGRGRARLIDTLKTDDHTSNQFITSESKENITKKTKGPESEAIASENGLFFPDLIYGSKGSSVNIYCNYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLNDHKDKLGGTKTFDGNTLYLPILLPNKMTVFISKAEDVELQIRILYKKKEEMRNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRPKIIPLAKLEVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQQNVEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCTSYVEYYKQYHNINIKDVNQPLIYSIKKSRGIPAERENLQFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGRSAEFSKHAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYEIPQFEMVCGNRIKISYIVVQKRINTRIFSGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRDVAEALSEKLFYL

Target ORF information:

RefSeq Version	NM_001043163.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster argonaute 3, transcript variant F (AGO3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043163.3

ORF Insert Sequence:

ATGTCTGGAA GAGGAAATTT GTTGAGCTTA TTTAATAAAA ATGCTGGCAA TATGGGAAAA 
TCAATTTCAA GTAAAGATCA TGAAATCGAC AGTGGCTTGG ATTTTAACAA CTCTGAATCA 
AGTGGAGAAC GTTTACTGTC AAGCCATAAT ATTGAAACTG ATCTTATAAC CACTCTTCAA 
CATGTAAACA TCTCGGTCGG CCGAGGACGT GCTAGGCTTA TAGACACATT AAAAACAGAT 
GACCATACAT CAAATCAATT TATAACATCT GAATCAAAGG AAAACATTAC CAAAAAGACA 
AAGGGCCCCG AATCGGAAGC AATTGCTTCA GAAAATGGAT TATTTTTTCC CGATTTAATT 
TACGGATCTA AAGGTTCATC TGTTAATATA TATTGTAATT ACTTAAAGCT AACGACGGAT 
GAATCCAAGG GAGTTTTTAA TTACGAAGTG CGTTTTTTTC CTCCCATTGA CTCTGTTCAT 
TTAAGAATAA AATATTTAAA TGATCATAAG GATAAACTCG GTGGAACCAA GACATTTGAC 
GGAAATACTT TATATTTACC TATTTTGTTA CCAAATAAGA TGACCGTCTT TATTTCAAAG 
GCGGAAGATG TTGAGTTGCA GATTCGCATT TTATACAAAA AAAAAGAGGA AATGAGGAAC 
TGCACGCAAT TATATAATAT TTTATTCGAT AGAGTTATGA AAGTATTGAA TTACGTAAAA 
TTTGATCGCA AGCAGTTTGA TCCTTCGCGT CCAAAAATAA TACCACTGGC AAAGTTAGAA 
GTTTGGCCTG GCTATGTTAC AGCCGTTGAC GAATACAAAG GTGGTTTAAT GCTATGCTGC 
GACGTTTCGC ATCGTATACT TTGCCAAAAA ACTGTTCTAG AAATGCTGGT TGATCTTTAT 
CAGCAAAATG TAGAGCATTA CCAAGAATCA GCAAGAAAAA TGTTGGTTGG AAATATTGTC 
TTAACTAGGT ATAACAATCG TACATATAAA ATAAATGACA TTTGTTTTGA TCAAAATCCA 
ACATGTCAGT TTGAAATTAA AACTGGCTGC ACAAGTTACG TGGAGTATTA CAAACAATAT 
CACAATATAA ACATAAAGGA CGTTAATCAA CCATTGATTT ACAGTATAAA GAAATCAAGA 
GGAATTCCAG CAGAAAGGGA AAATTTACAG TTTTGTTTAA TTCCAGAATT GTGTTATCTT 
ACTGGGCTGC GAGACGAAGT ACGGTCAGAT AACAAGCTTA TGCGCGAGAT TGCTACATTT 
ACAAGAGTTT CGCCAAATCA ACGACAGATG GCGTTAAATA AATTTTACGA AAATGTTTCA 
AATACACCCG CCGCACAAGA AATTCTAAAT AGCTGGGGTC TTTCATTAAC GAATAATTCC 
AACAAGATAA GTGGGCGTCA AATGGACATA GAGCAAATAT ATTTTTCAAA AATTAGTGTA 
TCGGCCGGAA GGTCCGCAGA GTTCTCCAAA CATGCTGTGA CCAATGAAAT GTTGAAAGTA 
GTCCACCTGA GTAAATGGAT CATTATACAT TTAAGAAATT ATAGACAAGC TGCAACTAGC 
TTGCTCGATA ACATGAAACA GGCTTGTGAG TCACTTGGTA TGAACATTTC TAACCCAACA 
ATGATTTCGT TAGATCATGA CCGAATCGAT GCCTACATTC AAGCACTGCG GCGAAATATA 
ACTATGAATA CGCAAATGGT CGTATGTATT TGTCATAACA GAAGAGATGA TCGTTATGCT 
GCAATAAAAA AAATATGTTG CTCGGAAATA CCTATACCAT CACAGGTTAT AAATGCTAAA 
ACTTTACAAA ATGATCTTAA AATTCGATCC GTTGTTCAAA AAATTGTTCT ACAAATGAAC 
TGTAAACTCG GAGGCTCATT ATGGACGGTA AAAATTCCAT TTAAAAATGT AATGATTTGC 
GGAATAGACT CATATCATGA CCCATCTAAT CGGGGGAATT CTGTTGCTGC ATTTGTGGCC 
TCCATAAATT CGTCGTATTC ACAGTGGTAC AGTAAAGCTG TTGTACAAAC AAAACGTGAA 
GAAATTGTTA ATGGGCTCTC TGCATCTTTT GAAATTGCAT TAAAAATGTA TCGTAAAAGG 
AATGGCAAAC TCCCCACAAA TATTATAATA TACAGAGATG GAATTGGAGA TGGACAGCTT 
TATACATGTC TTAATTATGA AATTCCACAA TTTGAAATGG TGTGTGGAAA TCGCATAAAA 
ATTTCATATA TTGTAGTCCA AAAACGAATT AACACTCGCA TCTTCTCGGG TAGCGGCATT 
CATCTAGAAA ACCCATTGCC AGGAACTGTC GTAGATCAAC ATATAACAAA ATCAAATATG 
TATGATTTTT TTTTGGTTTC GCAATTGGTA CGACAGGGTA CTGTAACCCC AACTCATTAT 
GTTGTTCTTC GAGATGACTG CAATTATGGA CCCGACATAA TTCAAAAACT AAGTTACAAA 
CTTTGTTTTT TATATTACAA TTGGGCTGGT ACAGTACGAA TTCCAGCTTG TTGTATGTAC 
GCTCACAAAT TGGCTTATTT GATTGGACAA AGTATTCAAC GAGATGTGGC TGAAGCACTT 
TCTGAAAAAC TATTTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGO3 ( NM_001043162.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036627.2 Drosophila melanogaster argonaute 3, transcript variant D (AGO3), mRNA.

Price & Availability↑

CloneID	OFa20047
Clone ID Related Accession (Same CDS sequence)	NM_001043164.3 , NM_001043163.3 , NM_001043162.3
Accession Version	NM_001043162.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	argonaute 3, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001043162.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGGAA GAGGAAATTT GTTGAGCTTA TTTAATAAAA ATGCTGGCAA TATGGGAAAA 
TCAATTTCAA GTAAAGATCA TGAAATCGAC AGTGGCTTGG ATTTTAACAA CTCTGAATCA 
AGTGGAGAAC GTTTACTGTC AAGCCATAAT ATTGAAACTG ATCTTATAAC CACTCTTCAA 
CATGTAAACA TCTCGGTCGG CCGAGGACGT GCTAGGCTTA TAGACACATT AAAAACAGAT 
GACCATACAT CAAATCAATT TATAACATCT GAATCAAAGG AAAACATTAC CAAAAAGACA 
AAGGGCCCCG AATCGGAAGC AATTGCTTCA GAAAATGGAT TATTTTTTCC CGATTTAATT 
TACGGATCTA AAGGTTCATC TGTTAATATA TATTGTAATT ACTTAAAGCT AACGACGGAT 
GAATCCAAGG GAGTTTTTAA TTACGAAGTG CGTTTTTTTC CTCCCATTGA CTCTGTTCAT 
TTAAGAATAA AATATTTAAA TGATCATAAG GATAAACTCG GTGGAACCAA GACATTTGAC 
GGAAATACTT TATATTTACC TATTTTGTTA CCAAATAAGA TGACCGTCTT TATTTCAAAG 
GCGGAAGATG TTGAGTTGCA GATTCGCATT TTATACAAAA AAAAAGAGGA AATGAGGAAC 
TGCACGCAAT TATATAATAT TTTATTCGAT AGAGTTATGA AAGTATTGAA TTACGTAAAA 
TTTGATCGCA AGCAGTTTGA TCCTTCGCGT CCAAAAATAA TACCACTGGC AAAGTTAGAA 
GTTTGGCCTG GCTATGTTAC AGCCGTTGAC GAATACAAAG GTGGTTTAAT GCTATGCTGC 
GACGTTTCGC ATCGTATACT TTGCCAAAAA ACTGTTCTAG AAATGCTGGT TGATCTTTAT 
CAGCAAAATG TAGAGCATTA CCAAGAATCA GCAAGAAAAA TGTTGGTTGG AAATATTGTC 
TTAACTAGGT ATAACAATCG TACATATAAA ATAAATGACA TTTGTTTTGA TCAAAATCCA 
ACATGTCAGT TTGAAATTAA AACTGGCTGC ACAAGTTACG TGGAGTATTA CAAACAATAT 
CACAATATAA ACATAAAGGA CGTTAATCAA CCATTGATTT ACAGTATAAA GAAATCAAGA 
GGAATTCCAG CAGAAAGGGA AAATTTACAG TTTTGTTTAA TTCCAGAATT GTGTTATCTT 
ACTGGGCTGC GAGACGAAGT ACGGTCAGAT AACAAGCTTA TGCGCGAGAT TGCTACATTT 
ACAAGAGTTT CGCCAAATCA ACGACAGATG GCGTTAAATA AATTTTACGA AAATGTTTCA 
AATACACCCG CCGCACAAGA AATTCTAAAT AGCTGGGGTC TTTCATTAAC GAATAATTCC 
AACAAGATAA GTGGGCGTCA AATGGACATA GAGCAAATAT ATTTTTCAAA AATTAGTGTA 
TCGGCCGGAA GGTCCGCAGA GTTCTCCAAA CATGCTGTGA CCAATGAAAT GTTGAAAGTA 
GTCCACCTGA GTAAATGGAT CATTATACAT TTAAGAAATT ATAGACAAGC TGCAACTAGC 
TTGCTCGATA ACATGAAACA GGCTTGTGAG TCACTTGGTA TGAACATTTC TAACCCAACA 
ATGATTTCGT TAGATCATGA CCGAATCGAT GCCTACATTC AAGCACTGCG GCGAAATATA 
ACTATGAATA CGCAAATGGT CGTATGTATT TGTCATAACA GAAGAGATGA TCGTTATGCT 
GCAATAAAAA AAATATGTTG CTCGGAAATA CCTATACCAT CACAGGTTAT AAATGCTAAA 
ACTTTACAAA ATGATCTTAA AATTCGATCC GTTGTTCAAA AAATTGTTCT ACAAATGAAC 
TGTAAACTCG GAGGCTCATT ATGGACGGTA AAAATTCCAT TTAAAAATGT AATGATTTGC 
GGAATAGACT CATATCATGA CCCATCTAAT CGGGGGAATT CTGTTGCTGC ATTTGTGGCC 
TCCATAAATT CGTCGTATTC ACAGTGGTAC AGTAAAGCTG TTGTACAAAC AAAACGTGAA 
GAAATTGTTA ATGGGCTCTC TGCATCTTTT GAAATTGCAT TAAAAATGTA TCGTAAAAGG 
AATGGCAAAC TCCCCACAAA TATTATAATA TACAGAGATG GAATTGGAGA TGGACAGCTT 
TATACATGTC TTAATTATGA AATTCCACAA TTTGAAATGG TGTGTGGAAA TCGCATAAAA 
ATTTCATATA TTGTAGTCCA AAAACGAATT AACACTCGCA TCTTCTCGGG TAGCGGCATT 
CATCTAGAAA ACCCATTGCC AGGAACTGTC GTAGATCAAC ATATAACAAA ATCAAATATG 
TATGATTTTT TTTTGGTTTC GCAATTGGTA CGACAGGGTA CTGTAACCCC AACTCATTAT 
GTTGTTCTTC GAGATGACTG CAATTATGGA CCCGACATAA TTCAAAAACT AAGTTACAAA 
CTTTGTTTTT TATATTACAA TTGGGCTGGT ACAGTACGAA TTCCAGCTTG TTGTATGTAC 
GCTCACAAAT TGGCTTATTT GATTGGACAA AGTATTCAAC GAGATGTGGC TGAAGCACTT 
TCTGAAAAAC TATTTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036627.2
CDS	128..2731
Translation	MSGRGNLLSLFNKNAGNMGKSISSKDHEIDSGLDFNNSESSGERLLSSHNIETDLITTLQHVNISVGRGRARLIDTLKTDDHTSNQFITSESKENITKKTKGPESEAIASENGLFFPDLIYGSKGSSVNIYCNYLKLTTDESKGVFNYEVRFFPPIDSVHLRIKYLNDHKDKLGGTKTFDGNTLYLPILLPNKMTVFISKAEDVELQIRILYKKKEEMRNCTQLYNILFDRVMKVLNYVKFDRKQFDPSRPKIIPLAKLEVWPGYVTAVDEYKGGLMLCCDVSHRILCQKTVLEMLVDLYQQNVEHYQESARKMLVGNIVLTRYNNRTYKINDICFDQNPTCQFEIKTGCTSYVEYYKQYHNINIKDVNQPLIYSIKKSRGIPAERENLQFCLIPELCYLTGLRDEVRSDNKLMREIATFTRVSPNQRQMALNKFYENVSNTPAAQEILNSWGLSLTNNSNKISGRQMDIEQIYFSKISVSAGRSAEFSKHAVTNEMLKVVHLSKWIIIHLRNYRQAATSLLDNMKQACESLGMNISNPTMISLDHDRIDAYIQALRRNITMNTQMVVCICHNRRDDRYAAIKKICCSEIPIPSQVINAKTLQNDLKIRSVVQKIVLQMNCKLGGSLWTVKIPFKNVMICGIDSYHDPSNRGNSVAAFVASINSSYSQWYSKAVVQTKREEIVNGLSASFEIALKMYRKRNGKLPTNIIIYRDGIGDGQLYTCLNYEIPQFEMVCGNRIKISYIVVQKRINTRIFSGSGIHLENPLPGTVVDQHITKSNMYDFFLVSQLVRQGTVTPTHYVVLRDDCNYGPDIIQKLSYKLCFLYYNWAGTVRIPACCMYAHKLAYLIGQSIQRDVAEALSEKLFYL

Target ORF information:

RefSeq Version	NM_001043162.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster argonaute 3, transcript variant D (AGO3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043162.3

ORF Insert Sequence:

ATGTCTGGAA GAGGAAATTT GTTGAGCTTA TTTAATAAAA ATGCTGGCAA TATGGGAAAA 
TCAATTTCAA GTAAAGATCA TGAAATCGAC AGTGGCTTGG ATTTTAACAA CTCTGAATCA 
AGTGGAGAAC GTTTACTGTC AAGCCATAAT ATTGAAACTG ATCTTATAAC CACTCTTCAA 
CATGTAAACA TCTCGGTCGG CCGAGGACGT GCTAGGCTTA TAGACACATT AAAAACAGAT 
GACCATACAT CAAATCAATT TATAACATCT GAATCAAAGG AAAACATTAC CAAAAAGACA 
AAGGGCCCCG AATCGGAAGC AATTGCTTCA GAAAATGGAT TATTTTTTCC CGATTTAATT 
TACGGATCTA AAGGTTCATC TGTTAATATA TATTGTAATT ACTTAAAGCT AACGACGGAT 
GAATCCAAGG GAGTTTTTAA TTACGAAGTG CGTTTTTTTC CTCCCATTGA CTCTGTTCAT 
TTAAGAATAA AATATTTAAA TGATCATAAG GATAAACTCG GTGGAACCAA GACATTTGAC 
GGAAATACTT TATATTTACC TATTTTGTTA CCAAATAAGA TGACCGTCTT TATTTCAAAG 
GCGGAAGATG TTGAGTTGCA GATTCGCATT TTATACAAAA AAAAAGAGGA AATGAGGAAC 
TGCACGCAAT TATATAATAT TTTATTCGAT AGAGTTATGA AAGTATTGAA TTACGTAAAA 
TTTGATCGCA AGCAGTTTGA TCCTTCGCGT CCAAAAATAA TACCACTGGC AAAGTTAGAA 
GTTTGGCCTG GCTATGTTAC AGCCGTTGAC GAATACAAAG GTGGTTTAAT GCTATGCTGC 
GACGTTTCGC ATCGTATACT TTGCCAAAAA ACTGTTCTAG AAATGCTGGT TGATCTTTAT 
CAGCAAAATG TAGAGCATTA CCAAGAATCA GCAAGAAAAA TGTTGGTTGG AAATATTGTC 
TTAACTAGGT ATAACAATCG TACATATAAA ATAAATGACA TTTGTTTTGA TCAAAATCCA 
ACATGTCAGT TTGAAATTAA AACTGGCTGC ACAAGTTACG TGGAGTATTA CAAACAATAT 
CACAATATAA ACATAAAGGA CGTTAATCAA CCATTGATTT ACAGTATAAA GAAATCAAGA 
GGAATTCCAG CAGAAAGGGA AAATTTACAG TTTTGTTTAA TTCCAGAATT GTGTTATCTT 
ACTGGGCTGC GAGACGAAGT ACGGTCAGAT AACAAGCTTA TGCGCGAGAT TGCTACATTT 
ACAAGAGTTT CGCCAAATCA ACGACAGATG GCGTTAAATA AATTTTACGA AAATGTTTCA 
AATACACCCG CCGCACAAGA AATTCTAAAT AGCTGGGGTC TTTCATTAAC GAATAATTCC 
AACAAGATAA GTGGGCGTCA AATGGACATA GAGCAAATAT ATTTTTCAAA AATTAGTGTA 
TCGGCCGGAA GGTCCGCAGA GTTCTCCAAA CATGCTGTGA CCAATGAAAT GTTGAAAGTA 
GTCCACCTGA GTAAATGGAT CATTATACAT TTAAGAAATT ATAGACAAGC TGCAACTAGC 
TTGCTCGATA ACATGAAACA GGCTTGTGAG TCACTTGGTA TGAACATTTC TAACCCAACA 
ATGATTTCGT TAGATCATGA CCGAATCGAT GCCTACATTC AAGCACTGCG GCGAAATATA 
ACTATGAATA CGCAAATGGT CGTATGTATT TGTCATAACA GAAGAGATGA TCGTTATGCT 
GCAATAAAAA AAATATGTTG CTCGGAAATA CCTATACCAT CACAGGTTAT AAATGCTAAA 
ACTTTACAAA ATGATCTTAA AATTCGATCC GTTGTTCAAA AAATTGTTCT ACAAATGAAC 
TGTAAACTCG GAGGCTCATT ATGGACGGTA AAAATTCCAT TTAAAAATGT AATGATTTGC 
GGAATAGACT CATATCATGA CCCATCTAAT CGGGGGAATT CTGTTGCTGC ATTTGTGGCC 
TCCATAAATT CGTCGTATTC ACAGTGGTAC AGTAAAGCTG TTGTACAAAC AAAACGTGAA 
GAAATTGTTA ATGGGCTCTC TGCATCTTTT GAAATTGCAT TAAAAATGTA TCGTAAAAGG 
AATGGCAAAC TCCCCACAAA TATTATAATA TACAGAGATG GAATTGGAGA TGGACAGCTT 
TATACATGTC TTAATTATGA AATTCCACAA TTTGAAATGG TGTGTGGAAA TCGCATAAAA 
ATTTCATATA TTGTAGTCCA AAAACGAATT AACACTCGCA TCTTCTCGGG TAGCGGCATT 
CATCTAGAAA ACCCATTGCC AGGAACTGTC GTAGATCAAC ATATAACAAA ATCAAATATG 
TATGATTTTT TTTTGGTTTC GCAATTGGTA CGACAGGGTA CTGTAACCCC AACTCATTAT 
GTTGTTCTTC GAGATGACTG CAATTATGGA CCCGACATAA TTCAAAAACT AAGTTACAAA 
CTTTGTTTTT TATATTACAA TTGGGCTGGT ACAGTACGAA TTCCAGCTTG TTGTATGTAC 
GCTCACAAAT TGGCTTATTT GATTGGACAA AGTATTCAAC GAGATGTGGC TGAAGCACTT 
TCTGAAAAAC TATTTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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AGO3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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