eIF4B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eIF4B
Entrez Gene ID	3355041
Full Name	Eukaryotic initiation factor 4B
Synonyms	CG10837,Dm-eIF4B,Dmel\CG10837,EIF-4B,Eif4B,eIF-4B-L,eIF-4B-S,eIF-4b,eIF4B,eIF4B-L,eIF4B-S,eIf4b
General protein information	Preferred Names Eukaryotic initiation factor 4B Names CG10837 gene product from transcript CG10837-RF CG10837-PA CG10837-PB CG10837-PE CG10837-PF eIF-4B-PA eIF-4B-PB eIF-4B-PE eIF-4B-PF eukaryotic initiation factor 4B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001015319.2	NP_001015319.1	eukaryotic translation initiation factor 4B, isoform B
NM_001276232.1	NP_001263161.1	eukaryotic translation initiation factor 4B, isoform F
NM_001015320.2	NP_001015320.1	eukaryotic translation initiation factor 4B, isoform E
NM_001015321.2	NP_001015321.1	eukaryotic translation initiation factor 4B, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that two functionally redundant isoforms of Drosophila eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival, and proliferation.
Title: Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival, and proliferation.

The following eIF4B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eIF4B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00053	NM_001015319.2 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4B (eIF4B), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa00054	NM_001276232.1 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4B (eIF4B), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa00055	NM_001015321.2 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4B (eIF4B), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa00056	NM_001015320.2 Latest version!	Drosophila melanogaster eukaryotic translation initiation factor 4B (eIF4B), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eIF4B ( NM_001015319.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001015319.1 Drosophila melanogaster eukaryotic translation initiation factor 4B (eIF4B), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00053
Clone ID Related Accession (Same CDS sequence)	NM_001015319.2
Accession Version	NM_001015319.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001015319.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTCCG CAGGAAAGAA GGGCAAAAAG AATAAAGGAA CAGTTATCTC CTTGCAATCA 
TTTCTATGTA ATTCTGACGC TCCAGTCGGA ACAACCCAAG TCTCAAAAAA AATACGAAAT 
TTAGATGGCG ATGATAGCGA TGATGGAAGT GGTACATTGC CGTTGGTTTA TCAGCTTCCA 
ACCGCTCCGA GGGCAAATCG GATATTCGAC GATAATTCTA TTCCTCATAA AGCCCCATTT 
ATTGCTTATA TCAATAATTT ACCATTTGAC GCAAATGAAG ATGACCTCTA CGAGTTCTTT 
GAAGGTATTA ATTTAATATC TCTGAGACTA CCAAGAGAAG ATGGCGAAAA TGGTCGTTCC 
AGAGGCTTTG GCTATGTGGA GTTGGAAAAT CGAGAGGATT TAATTCATGT ACTTAGCTTG 
CCAGACCCCT CTATTAAAGG TCGCCGCATT CGCATTGAGC TATCGAATGA AAACGACCAG 
CAAAGCCGCC AAAAAAGTAA TAGGCGGTTT GATGGTTTTG GAAATAATGG AGATAATAGG 
GATTCCGGAA ATTGGAGGCG AGACAGCCAA AACAACGGCA GTAACTTTGG TTATTCTTCC 
AATTTTGAAA GAAGCTTTAA TAGAGAGAGA AAATCTTTAC CAGATAGAGA TGATGTGAAC 
ACTCCTGGAT CTTGGCGCAC GAGCGCAAGG CCGCAATCTA TTGATACTTC CCCTACACGC 
AGAGAAGTTG AACAAGTATC TGAAAAATAT CGCGAAGGTC GAGTCAAAAT AGCAGACAGA 
TATTCCCGAG AAGAGACATC CAAAGTTGAA GAAGAAAGGC CAAAATTGAA TTTAAAACCA 
AGAACTTTAC CATTGCCAGA TGTAAAGACA ATTGAATTCG AAAAATGTGA CGTTGATGAA 
TTTAATTTGG ATAAACAAGG CGGAACTTCA TCATTAAACG TGTTTGGGTC TGCAAAACCG 
GTAGATACAG CAGCAAGGGA GTTGGAGATT GAGCAAAGAT TGGCTCTCGC TCGAAAACAG 
GATAAGGGGC GCAGAGAACA GGGTTTAAAT GAAAAACTTG CCGAGCTCCA GGTGGATAAA 
AATGATAATG AAAGTCTAAG TGCAACAGTT AGTTGGCGCA CGAATCAGGA CTACAAGGAA 
AGCGACAAAA TTAATAAGTG TCCAGGTGAA AAACAGCGGG ACGAGGAAAT CCATAGATTG 
AATCATGCCC GAGGCCAGTT TAACAGGCCG GAACTTTTGA GAGAAAAACC TAATGCGAAC 
GGAGAGAGCA AACGAGATTG TCGCAATGAC CGTTCTGTGT CGAAGAAAAC TTCCCAGAAT 
GATCTTGTTT TGCAATCATC TAATAAATAT TCAGGACTCG ACAATGAAGG ATCGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015319.1
CDS	69..1448
Translation	MASAGKKGKKNKGTVISLQSFLCNSDAPVGTTQVSKKIRNLDGDDSDDGSGTLPLVYQLPTAPRANRIFDDNSIPHKAPFIAYINNLPFDANEDDLYEFFEGINLISLRLPREDGENGRSRGFGYVELENREDLIHVLSLPDPSIKGRRIRIELSNENDQQSRQKSNRRFDGFGNNGDNRDSGNWRRDSQNNGSNFGYSSNFERSFNRERKSLPDRDDVNTPGSWRTSARPQSIDTSPTRREVEQVSEKYREGRVKIADRYSREETSKVEEERPKLNLKPRTLPLPDVKTIEFEKCDVDEFNLDKQGGTSSLNVFGSAKPVDTAARELEIEQRLALARKQDKGRREQGLNEKLAELQVDKNDNESLSATVSWRTNQDYKESDKINKCPGEKQRDEEIHRLNHARGQFNRPELLREKPNANGESKRDCRNDRSVSKKTSQNDLVLQSSNKYSGLDNEGSE

Target ORF information:

RefSeq Version	NM_001015319.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4B (eIF4B), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015319.2

ORF Insert Sequence:

ATGGCGTCCG CAGGAAAGAA GGGCAAAAAG AATAAAGGAA CAGTTATCTC CTTGCAATCA 
TTTCTATGTA ATTCTGACGC TCCAGTCGGA ACAACCCAAG TCTCAAAAAA AATACGAAAT 
TTAGATGGCG ATGATAGCGA TGATGGAAGT GGTACATTGC CGTTGGTTTA TCAGCTTCCA 
ACCGCTCCGA GGGCAAATCG GATATTCGAC GATAATTCTA TTCCTCATAA AGCCCCATTT 
ATTGCTTATA TCAATAATTT ACCATTTGAC GCAAATGAAG ATGACCTCTA CGAGTTCTTT 
GAAGGTATTA ATTTAATATC TCTGAGACTA CCAAGAGAAG ATGGCGAAAA TGGTCGTTCC 
AGAGGCTTTG GCTATGTGGA GTTGGAAAAT CGAGAGGATT TAATTCATGT ACTTAGCTTG 
CCAGACCCCT CTATTAAAGG TCGCCGCATT CGCATTGAGC TATCGAATGA AAACGACCAG 
CAAAGCCGCC AAAAAAGTAA TAGGCGGTTT GATGGTTTTG GAAATAATGG AGATAATAGG 
GATTCCGGAA ATTGGAGGCG AGACAGCCAA AACAACGGCA GTAACTTTGG TTATTCTTCC 
AATTTTGAAA GAAGCTTTAA TAGAGAGAGA AAATCTTTAC CAGATAGAGA TGATGTGAAC 
ACTCCTGGAT CTTGGCGCAC GAGCGCAAGG CCGCAATCTA TTGATACTTC CCCTACACGC 
AGAGAAGTTG AACAAGTATC TGAAAAATAT CGCGAAGGTC GAGTCAAAAT AGCAGACAGA 
TATTCCCGAG AAGAGACATC CAAAGTTGAA GAAGAAAGGC CAAAATTGAA TTTAAAACCA 
AGAACTTTAC CATTGCCAGA TGTAAAGACA ATTGAATTCG AAAAATGTGA CGTTGATGAA 
TTTAATTTGG ATAAACAAGG CGGAACTTCA TCATTAAACG TGTTTGGGTC TGCAAAACCG 
GTAGATACAG CAGCAAGGGA GTTGGAGATT GAGCAAAGAT TGGCTCTCGC TCGAAAACAG 
GATAAGGGGC GCAGAGAACA GGGTTTAAAT GAAAAACTTG CCGAGCTCCA GGTGGATAAA 
AATGATAATG AAAGTCTAAG TGCAACAGTT AGTTGGCGCA CGAATCAGGA CTACAAGGAA 
AGCGACAAAA TTAATAAGTG TCCAGGTGAA AAACAGCGGG ACGAGGAAAT CCATAGATTG 
AATCATGCCC GAGGCCAGTT TAACAGGCCG GAACTTTTGA GAGAAAAACC TAATGCGAAC 
GGAGAGAGCA AACGAGATTG TCGCAATGAC CGTTCTGTGT CGAAGAAAAC TTCCCAGAAT 
GATCTTGTTT TGCAATCATC TAATAAATAT TCAGGACTCG ACAATGAAGG ATCGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF4B ( NM_001276232.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263161.1 Drosophila melanogaster eukaryotic translation initiation factor 4B (eIF4B), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa00054
Clone ID Related Accession (Same CDS sequence)	NM_001276232.1
Accession Version	NM_001276232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4B, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTCCG CAGGAAAGAA GGGCAAAAAG AATAAAGGAA CAGTTATCTC CTTGCAATCA 
TTTCTATGTA ATTCTGACGC TCCAGTCGGA ACAACCCAAG TCTCAAAAAA AATACGAAAT 
TTAGATGGCG ATGATAGCGA TGATGGAAGT GGTACATTGC CGTTGGTTTA TCAGCTTCCA 
ACCGCTCCGA GGGCAAATCG GATATTCGAC GATAATTCTA TTCCTCATAA AGCCCCATTT 
ATTGCTTATA TCAATAATTT ACCATTTGAC GCAAATGAAG ATGACCTCTA CGAGTTCTTT 
GAAGGTATTA ATTTAATATC TCTGAGACTA CCAAGAGAAG ATGGCGAAAA TGGTCGTTCC 
AGAGGCTTTG GCTATGTGGA GTTGGAAAAT CGAGAGGATT TAATTCATGT ACTTAGCTTG 
CCAGACCCCT CTATTAAAGG TCGCCGCATT CGCATTGAGC TATCGAATGA AAACGACCAG 
CAAAGCCGCC AAAAAAGTAA TAGGCGGTTT GATGGTTTTG GAAATAATGG AGATAATAGG 
GATTCCGGAA ATTGGAGGCG AGACAGCCAA AACAACGGCA GTAACTTTGG TTATTCTTCC 
AATTTTGAAA GAAGCTTTAA TAGAGAGAGA AAATCTTTAC CAGATAGAGA TGATGTGAAC 
ACTCCTGGAT CTTGGCGCAC GAGCGCAAGG CCGCAATCTA TTGATACTTC CCCTACACGC 
AGAGAAGTTG AACAAGTATC TGAAAAATAT CGCGAAGGTC GAGTCAAAAT AGCAGACAGA 
TATTCCCGAG AAGAGACATC CAAAGTTGAA GAAGAAAGGC CAAAATTGAA TTTAAAACCA 
AGAACTTTAC CATTGCCAGA TGTAAAGACA ATTGAATTCG AAAAATGTGA CGTTGATGAA 
TTTAATTTGG ATAAACAAGG CGGAACTTCA TCATTAAACG TGTTTGGGTC TGCAAAACCG 
GTAGATACAG CAGCAAGGGA GTTGGAGATT GAGCAAAGAT TGGCTCTCGC TCGAAAACAG 
GATAAGGGGC GCAGAGAACA GGGTTTAAAT GAAAAACTTG CCGAGCTCCA GGTGGATAAA 
AATGATAATG AAAGTCTAAG TGCAACAGTT AGTTGGCGCA CGAATCAGGA CTACAAGGAA 
AGCGACAAAA TTAATAAGTG TCCAGGCCGG AACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263161.1
CDS	69..1247
Translation	MASAGKKGKKNKGTVISLQSFLCNSDAPVGTTQVSKKIRNLDGDDSDDGSGTLPLVYQLPTAPRANRIFDDNSIPHKAPFIAYINNLPFDANEDDLYEFFEGINLISLRLPREDGENGRSRGFGYVELENREDLIHVLSLPDPSIKGRRIRIELSNENDQQSRQKSNRRFDGFGNNGDNRDSGNWRRDSQNNGSNFGYSSNFERSFNRERKSLPDRDDVNTPGSWRTSARPQSIDTSPTRREVEQVSEKYREGRVKIADRYSREETSKVEEERPKLNLKPRTLPLPDVKTIEFEKCDVDEFNLDKQGGTSSLNVFGSAKPVDTAARELEIEQRLALARKQDKGRREQGLNEKLAELQVDKNDNESLSATVSWRTNQDYKESDKINKCPGRNF

Target ORF information:

RefSeq Version	NM_001276232.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4B (eIF4B), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276232.1

ORF Insert Sequence:

ATGGCGTCCG CAGGAAAGAA GGGCAAAAAG AATAAAGGAA CAGTTATCTC CTTGCAATCA 
TTTCTATGTA ATTCTGACGC TCCAGTCGGA ACAACCCAAG TCTCAAAAAA AATACGAAAT 
TTAGATGGCG ATGATAGCGA TGATGGAAGT GGTACATTGC CGTTGGTTTA TCAGCTTCCA 
ACCGCTCCGA GGGCAAATCG GATATTCGAC GATAATTCTA TTCCTCATAA AGCCCCATTT 
ATTGCTTATA TCAATAATTT ACCATTTGAC GCAAATGAAG ATGACCTCTA CGAGTTCTTT 
GAAGGTATTA ATTTAATATC TCTGAGACTA CCAAGAGAAG ATGGCGAAAA TGGTCGTTCC 
AGAGGCTTTG GCTATGTGGA GTTGGAAAAT CGAGAGGATT TAATTCATGT ACTTAGCTTG 
CCAGACCCCT CTATTAAAGG TCGCCGCATT CGCATTGAGC TATCGAATGA AAACGACCAG 
CAAAGCCGCC AAAAAAGTAA TAGGCGGTTT GATGGTTTTG GAAATAATGG AGATAATAGG 
GATTCCGGAA ATTGGAGGCG AGACAGCCAA AACAACGGCA GTAACTTTGG TTATTCTTCC 
AATTTTGAAA GAAGCTTTAA TAGAGAGAGA AAATCTTTAC CAGATAGAGA TGATGTGAAC 
ACTCCTGGAT CTTGGCGCAC GAGCGCAAGG CCGCAATCTA TTGATACTTC CCCTACACGC 
AGAGAAGTTG AACAAGTATC TGAAAAATAT CGCGAAGGTC GAGTCAAAAT AGCAGACAGA 
TATTCCCGAG AAGAGACATC CAAAGTTGAA GAAGAAAGGC CAAAATTGAA TTTAAAACCA 
AGAACTTTAC CATTGCCAGA TGTAAAGACA ATTGAATTCG AAAAATGTGA CGTTGATGAA 
TTTAATTTGG ATAAACAAGG CGGAACTTCA TCATTAAACG TGTTTGGGTC TGCAAAACCG 
GTAGATACAG CAGCAAGGGA GTTGGAGATT GAGCAAAGAT TGGCTCTCGC TCGAAAACAG 
GATAAGGGGC GCAGAGAACA GGGTTTAAAT GAAAAACTTG CCGAGCTCCA GGTGGATAAA 
AATGATAATG AAAGTCTAAG TGCAACAGTT AGTTGGCGCA CGAATCAGGA CTACAAGGAA 
AGCGACAAAA TTAATAAGTG TCCAGGCCGG AACTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF4B ( NM_001015321.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001015321.1 Drosophila melanogaster eukaryotic translation initiation factor 4B (eIF4B), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00055
Clone ID Related Accession (Same CDS sequence)	NM_001015321.2
Accession Version	NM_001015321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4B, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001015321.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTCCG CAGGAAAGAA GGGCAAAAAG AATAAAGGAA CAGTTATCTC CTTGCAATCA 
TTTCTATGTA ATTCTGACGC TCCAGTCGGA ACAACCCAAG TCTCAAAAAA AATACGAAAT 
TTAGATGGCG ATGATAGCGA TGATGGAAGT GGTACATTGC CGTTGGTTTA TCAGCTTCCA 
ACCGCTCCGA GGGCAAATCG GATATTCGAC GATAATTCTA TTCCTCATAA AGCCCCATTT 
ATTGCTTATA TCAATAATTT ACCATTTGAC GCAAATGAAG ATGACCTCTA CGAGTTCTTT 
GAAGGTATTA ATTTAATATC TCTGAGACTA CCAAGAGAAG ATGGCGAAAA TGGTCGTTCC 
AGAGGCTTTG GCTATGTGGA GTTGGAAAAT CGAGAGGATT TAATTCATGT ACTTAGCTTG 
CCAGACCCCT CTATTAAAGG TCGCCGCATT CGCATTGAGC TATCGAATGA AAACGACCAG 
CAAAGCCGCC AAAAAAGTAA TAGGCGGTTT GATGGTTTTG GAAATAATGG AGATAATAGG 
GATTCCGGAA ATTGGAGGCG AGACAGCCAA AACAACGGCA GTAACTTTGG TTATTCTTCC 
AATTTTGAAA GAAGCTTTAA TAGAGAGAGA AAATCTTTAC CAGATAGAGA TGATGTGAAC 
ACTCCTGGAT CTTGGCGCAC GAGCGCAAGG CCGCAATCTA TTGATACTTC CCCTACACGC 
AGAGAAGTTG AACAAGTATC TGAAAAATAT CGCGAAGGTC GAGTCAAAAT AGCAGACAGA 
TATTCCCGAG AAGAGACATC CAAAGTTGAA GAAGAAAGGC CAAAATTGAA TTTAAAACCA 
AGAACTTTAC CATTGCCAGA TGTAAAGACA ATTGAATTCG AAAAATGTGA CGTTGATGAA 
TTTAATTTGG ATAAACAAGG CGGAACTTCA TCATTAAACG TGTTTGGGTC TGCAAAACCG 
GTAGATACAG CAGCAAGGGA GTTGGAGATT GAGCAAAGAT TGGCTCTCGC TCGAAAACAG 
GATAAGGGGC GCAGAGAACA GGGTTTAAAT GAAAAACTTG CCGAGCTCCA GGTGGATAAA 
AATGATAATG AAAGTCTAAG TGCAACAGTT AGTTGGCGCA CGAATCAGGA CTACAAGGAA 
AGCGACAAAA TTAATAAGTG TCCAGGTAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015321.1
CDS	69..1241
Translation	MASAGKKGKKNKGTVISLQSFLCNSDAPVGTTQVSKKIRNLDGDDSDDGSGTLPLVYQLPTAPRANRIFDDNSIPHKAPFIAYINNLPFDANEDDLYEFFEGINLISLRLPREDGENGRSRGFGYVELENREDLIHVLSLPDPSIKGRRIRIELSNENDQQSRQKSNRRFDGFGNNGDNRDSGNWRRDSQNNGSNFGYSSNFERSFNRERKSLPDRDDVNTPGSWRTSARPQSIDTSPTRREVEQVSEKYREGRVKIADRYSREETSKVEEERPKLNLKPRTLPLPDVKTIEFEKCDVDEFNLDKQGGTSSLNVFGSAKPVDTAARELEIEQRLALARKQDKGRREQGLNEKLAELQVDKNDNESLSATVSWRTNQDYKESDKINKCPGN

Target ORF information:

RefSeq Version	NM_001015321.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4B (eIF4B), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015321.2

ORF Insert Sequence:

ATGGCGTCCG CAGGAAAGAA GGGCAAAAAG AATAAAGGAA CAGTTATCTC CTTGCAATCA 
TTTCTATGTA ATTCTGACGC TCCAGTCGGA ACAACCCAAG TCTCAAAAAA AATACGAAAT 
TTAGATGGCG ATGATAGCGA TGATGGAAGT GGTACATTGC CGTTGGTTTA TCAGCTTCCA 
ACCGCTCCGA GGGCAAATCG GATATTCGAC GATAATTCTA TTCCTCATAA AGCCCCATTT 
ATTGCTTATA TCAATAATTT ACCATTTGAC GCAAATGAAG ATGACCTCTA CGAGTTCTTT 
GAAGGTATTA ATTTAATATC TCTGAGACTA CCAAGAGAAG ATGGCGAAAA TGGTCGTTCC 
AGAGGCTTTG GCTATGTGGA GTTGGAAAAT CGAGAGGATT TAATTCATGT ACTTAGCTTG 
CCAGACCCCT CTATTAAAGG TCGCCGCATT CGCATTGAGC TATCGAATGA AAACGACCAG 
CAAAGCCGCC AAAAAAGTAA TAGGCGGTTT GATGGTTTTG GAAATAATGG AGATAATAGG 
GATTCCGGAA ATTGGAGGCG AGACAGCCAA AACAACGGCA GTAACTTTGG TTATTCTTCC 
AATTTTGAAA GAAGCTTTAA TAGAGAGAGA AAATCTTTAC CAGATAGAGA TGATGTGAAC 
ACTCCTGGAT CTTGGCGCAC GAGCGCAAGG CCGCAATCTA TTGATACTTC CCCTACACGC 
AGAGAAGTTG AACAAGTATC TGAAAAATAT CGCGAAGGTC GAGTCAAAAT AGCAGACAGA 
TATTCCCGAG AAGAGACATC CAAAGTTGAA GAAGAAAGGC CAAAATTGAA TTTAAAACCA 
AGAACTTTAC CATTGCCAGA TGTAAAGACA ATTGAATTCG AAAAATGTGA CGTTGATGAA 
TTTAATTTGG ATAAACAAGG CGGAACTTCA TCATTAAACG TGTTTGGGTC TGCAAAACCG 
GTAGATACAG CAGCAAGGGA GTTGGAGATT GAGCAAAGAT TGGCTCTCGC TCGAAAACAG 
GATAAGGGGC GCAGAGAACA GGGTTTAAAT GAAAAACTTG CCGAGCTCCA GGTGGATAAA 
AATGATAATG AAAGTCTAAG TGCAACAGTT AGTTGGCGCA CGAATCAGGA CTACAAGGAA 
AGCGACAAAA TTAATAAGTG TCCAGGTAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eIF4B ( NM_001015320.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001015320.1 Drosophila melanogaster eukaryotic translation initiation factor 4B (eIF4B), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa00056
Clone ID Related Accession (Same CDS sequence)	NM_001015320.2
Accession Version	NM_001015320.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation initiation factor 4B, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001015320.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTCCG CAGGAAAGAA GGGCAAAAAG AATAAAGGAA CAGTTATCTC CTTGCAATCA 
TTTCTATGTA ATTCTGACGC TCCAGTCGGA ACAACCCAAG TCTCAAAAAA AATACGAAAT 
TTAGATGGCG ATGATAGCGA TGATGGAAGT GGTACATTGC CGTTGGTTTA TCAGCTTCCA 
ACCGCTCCGA GGGCAAATCG GATATTCGAC GATAATTCTA TTCCTCATAA AGCCCCATTT 
ATTGCTTATA TCAATAATTT ACCATTTGAC GCAAATGAAG ATGACCTCTA CGAGTTCTTT 
GAAGGTATTA ATTTAATATC TCTGAGACTA CCAAGAGAAG ATGGCGAAAA TGGTCGTTCC 
AGAGGCTTTG GCTATGTGGA GTTGGAAAAT CGAGAGGATT TAATTCATGT ACTTAGCTTG 
CCAGACCCCT CTATTAAAGG TCGCCGCATT CGCATTGAGC TATCGAATGA AAACGACCAG 
CAAAGCCGCC AAAAAAGTAA TAGGCGGTTT GATGGTTTTG GAAATAATGG AGATAATAGG 
GATTCCGGAA ATTGGAGGCG AGACAGCCAA AACAACGGCA GTAACTTTGG TTATTCTTCC 
AATTTTGAAA GAAGCTTTAA TAGAGAGAGA AAATCTTTAC CAGATAGAGA TGATGTGAAC 
ACTCCTGGAT CTTGGCGCAC GAGCGCAAGG CCGCAATCTA TTGATACTTC CCCTACACGC 
AGAGAAGTTG AACAAGTATC TGAAAAATAT CGCGAAGGTC GAGTCAAAAT AGCAGACAGA 
TATTCCCGAG AAGAGACATC CAAAGTTGAA GAAGAAAGGC CAAAATTGAA TTTAAAACCA 
AGAACTTTAC CATTGCCAGA TGTAAAGACA ATTGAATTCG AAAAATGTGA CGTTGATGAA 
TTTAATTTGG ATAAACAAGG CGGAACTTCA TCATTAAACG TGTTTGGGTC TGCAAAACCG 
GTAGATACAG CAGCAAGGGA GTTGGAGATT GAGCAAAGAT TGGCTCTCGC TCGAAAACAG 
GATAAGGGGC GCAGAGAACA GGGTTTAAAT GAAAAACTTG CCGAGCTCCA GGTGGATAAA 
AATGATAATG AAAGTCTAAG TGCAACAGTT AGTTGGCGCA CGAATCAGGA CTACAAGGAA 
AGCGACAAAA TTAATAAGTG TCCAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015320.1
CDS	69..1238
Translation	MASAGKKGKKNKGTVISLQSFLCNSDAPVGTTQVSKKIRNLDGDDSDDGSGTLPLVYQLPTAPRANRIFDDNSIPHKAPFIAYINNLPFDANEDDLYEFFEGINLISLRLPREDGENGRSRGFGYVELENREDLIHVLSLPDPSIKGRRIRIELSNENDQQSRQKSNRRFDGFGNNGDNRDSGNWRRDSQNNGSNFGYSSNFERSFNRERKSLPDRDDVNTPGSWRTSARPQSIDTSPTRREVEQVSEKYREGRVKIADRYSREETSKVEEERPKLNLKPRTLPLPDVKTIEFEKCDVDEFNLDKQGGTSSLNVFGSAKPVDTAARELEIEQRLALARKQDKGRREQGLNEKLAELQVDKNDNESLSATVSWRTNQDYKESDKINKCPA

Target ORF information:

RefSeq Version	NM_001015320.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation initiation factor 4B (eIF4B), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015320.2

ORF Insert Sequence:

ATGGCGTCCG CAGGAAAGAA GGGCAAAAAG AATAAAGGAA CAGTTATCTC CTTGCAATCA 
TTTCTATGTA ATTCTGACGC TCCAGTCGGA ACAACCCAAG TCTCAAAAAA AATACGAAAT 
TTAGATGGCG ATGATAGCGA TGATGGAAGT GGTACATTGC CGTTGGTTTA TCAGCTTCCA 
ACCGCTCCGA GGGCAAATCG GATATTCGAC GATAATTCTA TTCCTCATAA AGCCCCATTT 
ATTGCTTATA TCAATAATTT ACCATTTGAC GCAAATGAAG ATGACCTCTA CGAGTTCTTT 
GAAGGTATTA ATTTAATATC TCTGAGACTA CCAAGAGAAG ATGGCGAAAA TGGTCGTTCC 
AGAGGCTTTG GCTATGTGGA GTTGGAAAAT CGAGAGGATT TAATTCATGT ACTTAGCTTG 
CCAGACCCCT CTATTAAAGG TCGCCGCATT CGCATTGAGC TATCGAATGA AAACGACCAG 
CAAAGCCGCC AAAAAAGTAA TAGGCGGTTT GATGGTTTTG GAAATAATGG AGATAATAGG 
GATTCCGGAA ATTGGAGGCG AGACAGCCAA AACAACGGCA GTAACTTTGG TTATTCTTCC 
AATTTTGAAA GAAGCTTTAA TAGAGAGAGA AAATCTTTAC CAGATAGAGA TGATGTGAAC 
ACTCCTGGAT CTTGGCGCAC GAGCGCAAGG CCGCAATCTA TTGATACTTC CCCTACACGC 
AGAGAAGTTG AACAAGTATC TGAAAAATAT CGCGAAGGTC GAGTCAAAAT AGCAGACAGA 
TATTCCCGAG AAGAGACATC CAAAGTTGAA GAAGAAAGGC CAAAATTGAA TTTAAAACCA 
AGAACTTTAC CATTGCCAGA TGTAAAGACA ATTGAATTCG AAAAATGTGA CGTTGATGAA 
TTTAATTTGG ATAAACAAGG CGGAACTTCA TCATTAAACG TGTTTGGGTC TGCAAAACCG 
GTAGATACAG CAGCAAGGGA GTTGGAGATT GAGCAAAGAT TGGCTCTCGC TCGAAAACAG 
GATAAGGGGC GCAGAGAACA GGGTTTAAAT GAAAAACTTG CCGAGCTCCA GGTGGATAAA 
AATGATAATG AAAGTCTAAG TGCAACAGTT AGTTGGCGCA CGAATCAGGA CTACAAGGAA 
AGCGACAAAA TTAATAAGTG TCCAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eIF4B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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