vtd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	vtd
Entrez Gene ID	3354896
Full Name	verthandi
Synonyms	80Fh,CG17436,CG40222,DRAD21,DRad21,DmRAD21,Dmel\CG17436,Drad21,RAD21,Rad21,Rad21/Scc1,Scc1,VTD,dRad21,drad21,l(3)80Fh,l(3)Lh3,l3,lethal 3,rad21,scc1
General protein information	Preferred Names verthandi Names CG17436 gene product from transcript CG17436-RB CG17436-PA CG17436-PB Rad21 alpha kleisin alpha-kleisin cohesin lethal 3 lethal(3)80Fh vtd-PA vtd-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 80F-80F\|3-46 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300711.1	NP_001287640.1	verthandi, isoform B
NM_001015132.2	NP_001015132.1	verthandi, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	RAD21	NP_006256.1
	RAD21	XP_001095142.1
Bos taurus (cattle)	RAD21	NP_001029889.1
Drosophila melanogaster (fruit fly)	vtd	NP_001015132.1
Canis lupus familiaris (dog)	RAD21	XP_539142.2
Mus musculus (house mouse)	Rad21	NP_033035.3
Gallus gallus (chicken)	RAD21	NP_001026121.1
Danio rerio (zebrafish)	LOC100536176	XP_003200317.1
Rattus norvegicus (Norway rat)	Rad21	NP_001020872.1
Danio rerio (zebrafish)	rad21b	NP_001038585.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004560	XP_313862.4
Xenopus tropicalis (tropical clawed frog)	rad21	XP_002940047.1
Pan troglodytes (chimpanzee)	RAD21	XP_003951384.1
Danio rerio (zebrafish)	rad21a	NP_955889.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila Yemanuclein associates with the cohesin and synaptonemal complexes
Title: Drosophila Yemanuclein associates with the cohesin and synaptonemal complexes.

We demonstrate that the lateral synaptonemal complex component C(2)M can interact directly with Rad21, potentially explaining why Rad21 is required for synaptonemal complex maintenance
Title: The cohesin subunit Rad21 is required for synaptonemal complex maintenance, but not sister chromatid cohesion, during Drosophila female meiosis.

These studies reveal a previously unrecognized role for PRC1 in facilitating productive gene transcription and provide new insights into how cohesin and PRC1 control development.
Title: Cohesin and polycomb proteins functionally interact to control transcription at silenced and active genes.

A direct role for cohesin in gene regulation and ecdysone response in Drosophila salivary glands.
Title: A direct role for cohesin in gene regulation and ecdysone response in Drosophila salivary glands.

Fruit flies whose Rad21 is cleaved by TEV protease show precocious separation of sister chromatids and massive chromosome missegregation in proliferating cells.
Title: Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons.

The following vtd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vtd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa22372	NM_001300711.1 Latest version!	Drosophila melanogaster verthandi (vtd), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa00011	NM_001015132.2 Latest version!	Drosophila melanogaster verthandi (vtd), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vtd ( NM_001300711.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287640.1 Drosophila melanogaster verthandi (vtd), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa22372
Clone ID Related Accession (Same CDS sequence)	NM_001300711.1
Accession Version	NM_001300711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	verthandi, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCTATG AGCACATTAT TTTGGCCAAG AAAGGCCCCC TAGCCAGGAT CTGGTTGGCA 
GCACATTGGG ACAAAAAAAT TACAAAGGCC CATGTATTTG AAACTAATAT TGAAAAATCA 
GTGGAAGGGA TTTTGCAACC AAAAGTAAAA TTAGCGTTGA GAACGTCTGG ACATTTATTG 
CTAGGTGTTG TTCGCATTTA TTCCAGAAAA GCGAAGTATT TATTAGCTGA TTGCAATGAA 
GCCTTTGTCA AGATTAAGAT GGCTTTTCGA CCAGGCATGG TCGACTTACC AGAAGGGCAC 
AGAGAAGCTA ATGTTAATGC AATAACACTC CCAGAAGTTT TTCATGACTT TGATACAGCC 
CTGCCCGAAT TAAACGATAT TGATATTGAG GCTCAGTTTT CTATAAATCA GTCCAGAGCT 
GACGAGATAA CAATGAGAGA AGATTACGGT AGTTTGTCTC TTTCCTTACA AGATGACGGA 
TTTGGTGATA TAGGCTTTGA AGCAGAAACG CCTGAAATTA TACGATGCTC TATACCTTCA 
AATATTAATG ATAAAATATT TGATAATGAC GTTTTGGAAA ATATTGAGTC TCTGGATCCA 
CATTCATTGG ACGCCCATGC TGATATGCCT GGTTCTCGAT TGGATGGTGA TGGGTTTGGA 
GATTCTTTTG GACAACCAGC CCTTTTTGAA GACGATCTTT TTGGTGATCC TTCTCAACCA 
GTTGAGCAGA TTACAAAAGA ATCTACTACT GTTCTCAACG CAGATGATTC TGATGAAGAC 
GCCATTGATA ATATTCATAA TGTCCCTTCG CCTGCAACCT CGCTCGTTAA TTCGATTGAA 
GATGAGAAAG AGGAAAATAA TTTAAATGGA CATGCAAGTG TGTCCGAAAA TGTGCCAATG 
AATGAAATAA CGCTTGTTCA AAACGAGGAT GAGGGATTTG CGTTAGCTCC TTTAGATGTC 
TCAATGTATA AAGGCGTTAC AAAAGCCAAA CGCAAAAGAA AACTTATTAT TGACGAAATA 
AAAAATATTT CTGGAGAGGA AATGAAGGCT CAGTTAGCCG ACACATCTGA TATATTAACC 
ACACTTGATT TAGCCCCACC AACAAAAAGA TTAATGTATT GGAAAGAAAC TGGAGGTGTC 
GAAAAACTTT TTTCATTACC ATCGCGATCC ATACCAGCTA GAGCTTTGTT TGGTAATTAC 
AATAGACAGT TGTTTTCGCA TTCAACATTC TTTGAGGACT TCTCGAGTGT TGTGCCAATG 
GAAATTCTTG CACTGGAATT TTATACCAAA GAGAACGAGA ACGCATTAAT TATTTTTAAT 
AAAAAAGGTC GCAAACGTAA GAATGATAAT ATGTCCAATT TGTTTTTGGA CCATGTCCCC 
GATTCAGTTG TTCAAAGTTT GGAAGCTCCG GAAGTCCTGA GAGCGAATCA TAAATCTCTA 
GGGGTATCTA CCGTTTCTGT GGAGATTGTA TCAAAAGAGC AAGAAAGCAT TTCATGTCAA 
AATGAATTAA CATTTTTTGA TAATATGCGA AGTCCGGACC TCTTGTCCTT AAATGAAATG 
GAACAGTTCT CATCAATAAA TGAGCTACCT TTAACACCAA GAAACATGAA TCATGAAATG 
GGTGACGATT TTAATCAAGG AGACTCAACG CCAGCTGGGT TGGATCATGG TCATGCCACT 
CCGCAGCATG GAAATATTGG CGAGATGGAT CACGACTCTG TTATACCGAC AAAAAAGACT 
GCAGTCATTT TAAATGAATC TGTGGGTACA TCTGTACTAA GTGACAATGG CGTTTCAAAA 
AGGACAAATA ATATTTTAAA AGGATGGGAC AACTATGAAA TTCCCAGTTT CGTTGGCCAG 
GGTATAAGAC ATGCAGGGCA CTGTTATCAG CAATATCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287640.1
CDS	88..1989
Translation	MFYEHIILAKKGPLARIWLAAHWDKKITKAHVFETNIEKSVEGILQPKVKLALRTSGHLLLGVVRIYSRKAKYLLADCNEAFVKIKMAFRPGMVDLPEGHREANVNAITLPEVFHDFDTALPELNDIDIEAQFSINQSRADEITMREDYGSLSLSLQDDGFGDIGFEAETPEIIRCSIPSNINDKIFDNDVLENIESLDPHSLDAHADMPGSRLDGDGFGDSFGQPALFEDDLFGDPSQPVEQITKESTTVLNADDSDEDAIDNIHNVPSPATSLVNSIEDEKEENNLNGHASVSENVPMNEITLVQNEDEGFALAPLDVSMYKGVTKAKRKRKLIIDEIKNISGEEMKAQLADTSDILTTLDLAPPTKRLMYWKETGGVEKLFSLPSRSIPARALFGNYNRQLFSHSTFFEDFSSVVPMEILALEFYTKENENALIIFNKKGRKRKNDNMSNLFLDHVPDSVVQSLEAPEVLRANHKSLGVSTVSVEIVSKEQESISCQNELTFFDNMRSPDLLSLNEMEQFSSINELPLTPRNMNHEMGDDFNQGDSTPAGLDHGHATPQHGNIGEMDHDSVIPTKKTAVILNESVGTSVLSDNGVSKRTNNILKGWDNYEIPSFVGQGIRHAGHCYQQYH

Target ORF information:

RefSeq Version	NM_001300711.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster verthandi (vtd), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300711.1

ORF Insert Sequence:

ATGTTCTATG AGCACATTAT TTTGGCCAAG AAAGGCCCCC TAGCCAGGAT CTGGTTGGCA 
GCACATTGGG ACAAAAAAAT TACAAAGGCC CATGTATTTG AAACTAATAT TGAAAAATCA 
GTGGAAGGGA TTTTGCAACC AAAAGTAAAA TTAGCGTTGA GAACGTCTGG ACATTTATTG 
CTAGGTGTTG TTCGCATTTA TTCCAGAAAA GCGAAGTATT TATTAGCTGA TTGCAATGAA 
GCCTTTGTCA AGATTAAGAT GGCTTTTCGA CCAGGCATGG TCGACTTACC AGAAGGGCAC 
AGAGAAGCTA ATGTTAATGC AATAACACTC CCAGAAGTTT TTCATGACTT TGATACAGCC 
CTGCCCGAAT TAAACGATAT TGATATTGAG GCTCAGTTTT CTATAAATCA GTCCAGAGCT 
GACGAGATAA CAATGAGAGA AGATTACGGT AGTTTGTCTC TTTCCTTACA AGATGACGGA 
TTTGGTGATA TAGGCTTTGA AGCAGAAACG CCTGAAATTA TACGATGCTC TATACCTTCA 
AATATTAATG ATAAAATATT TGATAATGAC GTTTTGGAAA ATATTGAGTC TCTGGATCCA 
CATTCATTGG ACGCCCATGC TGATATGCCT GGTTCTCGAT TGGATGGTGA TGGGTTTGGA 
GATTCTTTTG GACAACCAGC CCTTTTTGAA GACGATCTTT TTGGTGATCC TTCTCAACCA 
GTTGAGCAGA TTACAAAAGA ATCTACTACT GTTCTCAACG CAGATGATTC TGATGAAGAC 
GCCATTGATA ATATTCATAA TGTCCCTTCG CCTGCAACCT CGCTCGTTAA TTCGATTGAA 
GATGAGAAAG AGGAAAATAA TTTAAATGGA CATGCAAGTG TGTCCGAAAA TGTGCCAATG 
AATGAAATAA CGCTTGTTCA AAACGAGGAT GAGGGATTTG CGTTAGCTCC TTTAGATGTC 
TCAATGTATA AAGGCGTTAC AAAAGCCAAA CGCAAAAGAA AACTTATTAT TGACGAAATA 
AAAAATATTT CTGGAGAGGA AATGAAGGCT CAGTTAGCCG ACACATCTGA TATATTAACC 
ACACTTGATT TAGCCCCACC AACAAAAAGA TTAATGTATT GGAAAGAAAC TGGAGGTGTC 
GAAAAACTTT TTTCATTACC ATCGCGATCC ATACCAGCTA GAGCTTTGTT TGGTAATTAC 
AATAGACAGT TGTTTTCGCA TTCAACATTC TTTGAGGACT TCTCGAGTGT TGTGCCAATG 
GAAATTCTTG CACTGGAATT TTATACCAAA GAGAACGAGA ACGCATTAAT TATTTTTAAT 
AAAAAAGGTC GCAAACGTAA GAATGATAAT ATGTCCAATT TGTTTTTGGA CCATGTCCCC 
GATTCAGTTG TTCAAAGTTT GGAAGCTCCG GAAGTCCTGA GAGCGAATCA TAAATCTCTA 
GGGGTATCTA CCGTTTCTGT GGAGATTGTA TCAAAAGAGC AAGAAAGCAT TTCATGTCAA 
AATGAATTAA CATTTTTTGA TAATATGCGA AGTCCGGACC TCTTGTCCTT AAATGAAATG 
GAACAGTTCT CATCAATAAA TGAGCTACCT TTAACACCAA GAAACATGAA TCATGAAATG 
GGTGACGATT TTAATCAAGG AGACTCAACG CCAGCTGGGT TGGATCATGG TCATGCCACT 
CCGCAGCATG GAAATATTGG CGAGATGGAT CACGACTCTG TTATACCGAC AAAAAAGACT 
GCAGTCATTT TAAATGAATC TGTGGGTACA TCTGTACTAA GTGACAATGG CGTTTCAAAA 
AGGACAAATA ATATTTTAAA AGGATGGGAC AACTATGAAA TTCCCAGTTT CGTTGGCCAG 
GGTATAAGAC ATGCAGGGCA CTGTTATCAG CAATATCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vtd ( NM_001015132.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001015132.1 Drosophila melanogaster verthandi (vtd), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00011
Clone ID Related Accession (Same CDS sequence)	NM_001015132.2
Accession Version	NM_001015132.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	verthandi, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001015132.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCTATG AGCACATTAT TTTGGCCAAG AAAGGCCCCC TAGCCAGGAT CTGGTTGGCA 
GCACATTGGG ACAAAAAAAT TACAAAGGCC CATGTATTTG AAACTAATAT TGAAAAATCA 
GTGGAAGGGA TTTTGCAACC AAAAGTAAAA TTAGCGTTGA GAACGTCTGG ACATTTATTG 
CTAGGTGTTG TTCGCATTTA TTCCAGAAAA GCGAAGTATT TATTAGCTGA TTGCAATGAA 
GCCTTTGTCA AGATTAAGAT GGCTTTTCGA CCAGGCATGG TCGACTTACC AGAAGGGCAC 
AGAGAAGCTA ATGTTAATGC AATAACACTC CCAGAAGTTT TTCATGACTT TGATACAGCC 
CTGCCCGAAT TAAACGATAT TGATATTGAG GCTCAGTTTT CTATAAATCA GTCCAGAGCT 
GACGAGATAA CAATGAGAGA AGATTACGGT AGTTTGTCTC TTTCCTTACA AGATGACGGA 
TTTGGTGATA TAGGCTTTGA AGCAGAAACG CCTGAAATTA TACGATGCTC TATACCTTCA 
AATATTAATG ATAAAATATT TGATAATGAC GTTTTGGAAA ATATTGAGTC TCTGGATCCA 
CATTCATTGG ACGCCCATGC TGATATGCCT GGTTCTCGAT TGGATGGTGA TGGGTTTGGA 
GATTCTTTTG GACAACCAGC CCTTTTTGAA GACGATCTTT TTGGTGATCC TTCTCAACCA 
GTTGAGCAGA TTACAAAAGA ATCTACTACT GTTCTCAACG CAGATGATTC TGATGAAGAC 
GCCATTGATA ATATTCATAA TGTCCCTTCG CCTGCAACCT CGCTCGTTAA TTCGATTGAA 
GATGAGAAAG AGGAAAATAA TTTAAATGGA CATGCAAGTG TGTCCGAAAA TGTGCCAATG 
AATGAAATAA CGCTTGTTCA AAACGAGGAT GAGGGATTTG CGTTAGCTCC TTTAGATGTC 
TCAATGTATA AAGGCGTTAC AAAAGCCAAA CGCAAAAGAA AACTTATTAT TGACGAAATA 
AAAAATATTT CTGGAGAGGA AATGAAGGCT CAGTTAGCCG ACACATCTGA TATATTAACC 
ACACTTGATT TAGCCCCACC AACAAAAAGA TTAATGTATT GGAAAGAAAC TGGAGGTGTC 
GAAAAACTTT TTTCATTACC ATCGCGATCC ATACCAGCTA GAGCTTTGTT TGGTAATTAC 
AATAGACAGT TGTTTTCGCA TTCAACATTC TTTGAGGACT TCTCGAGTGT TGTGCCAATG 
GAAATTCTTG CACTGGAATT TTATACCAAA GAGAACGAGA ACGCATTAAT TATTTTTAAT 
AAAAAAGGTC GCAAACGTAA GAATGATAAT ATGTCCAATT TGTTTTTGGA CCATGTCCCC 
GATTCAGTTG TTCAAAGTTT GGAAGCTCCG GAAGTCCTGA GAGCGAATCA TAAATCTCTA 
GGGGTATCTA CCGTTTCTGT GGAGATTGTA TCAAAAGAGC AAGAAAGCAT TTCATGTCAA 
AATGAATTAA CATTTTTTGA TAATATGCGA AGTCCGGACC TCTTGTCCTT AAATGAAATG 
GAACAGTTCT CATCAATAAA TGAGCTACCT TTAACACCAA GAAACATGAA TCATGAAATG 
GGTGACGATT TTAATCAAGG AGACTCAACG CCAGCTGGGT TGGATCATGG TCATGCCACT 
CCGCAGCATG GAAATATTGG CGAGATGGAT CACGACTCTG TTATACCGAC AAAAAAGACT 
GCAGTCATTT TAAATGAATC TGTGGGTACA TCTGTACTAA GTGACAATGG CGTTTCAAAA 
AGGACAAATA ATATTTTAAA AGGATGGGAC AACTATGAAA TTCCCAGTTT CGTTGGCCAG 
AATCATGGTG ATGAACAAAT GGAAAATGAA ACCGATGAGC AATTTGAAGA ACGTGTTTTA 
AACAAGAGAG CCGCCCACCT TTTTATTGAC GTAAGAGCAC ATTTTATCGC CAAGGACAGC 
TTGGAACTTT CTCAGCTTAC GTCAGGAAAT TCCAGAAAAC AGGCGGCACA AAAATTTTAT 
TCCCTCCTCG TCTTAAAGAA GTTTAAGGTG CTTCACATTG ATCAATCTGC TCCCTATGCA 
GATATTACTA TTACTCGAGG ACCAACGTTC GAAAACCCAA AAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015132.1
CDS	88..2235
Translation	MFYEHIILAKKGPLARIWLAAHWDKKITKAHVFETNIEKSVEGILQPKVKLALRTSGHLLLGVVRIYSRKAKYLLADCNEAFVKIKMAFRPGMVDLPEGHREANVNAITLPEVFHDFDTALPELNDIDIEAQFSINQSRADEITMREDYGSLSLSLQDDGFGDIGFEAETPEIIRCSIPSNINDKIFDNDVLENIESLDPHSLDAHADMPGSRLDGDGFGDSFGQPALFEDDLFGDPSQPVEQITKESTTVLNADDSDEDAIDNIHNVPSPATSLVNSIEDEKEENNLNGHASVSENVPMNEITLVQNEDEGFALAPLDVSMYKGVTKAKRKRKLIIDEIKNISGEEMKAQLADTSDILTTLDLAPPTKRLMYWKETGGVEKLFSLPSRSIPARALFGNYNRQLFSHSTFFEDFSSVVPMEILALEFYTKENENALIIFNKKGRKRKNDNMSNLFLDHVPDSVVQSLEAPEVLRANHKSLGVSTVSVEIVSKEQESISCQNELTFFDNMRSPDLLSLNEMEQFSSINELPLTPRNMNHEMGDDFNQGDSTPAGLDHGHATPQHGNIGEMDHDSVIPTKKTAVILNESVGTSVLSDNGVSKRTNNILKGWDNYEIPSFVGQNHGDEQMENETDEQFEERVLNKRAAHLFIDVRAHFIAKDSLELSQLTSGNSRKQAAQKFYSLLVLKKFKVLHIDQSAPYADITITRGPTFENPKI

Target ORF information:

RefSeq Version	NM_001015132.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster verthandi (vtd), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015132.2

ORF Insert Sequence:

ATGTTCTATG AGCACATTAT TTTGGCCAAG AAAGGCCCCC TAGCCAGGAT CTGGTTGGCA 
GCACATTGGG ACAAAAAAAT TACAAAGGCC CATGTATTTG AAACTAATAT TGAAAAATCA 
GTGGAAGGGA TTTTGCAACC AAAAGTAAAA TTAGCGTTGA GAACGTCTGG ACATTTATTG 
CTAGGTGTTG TTCGCATTTA TTCCAGAAAA GCGAAGTATT TATTAGCTGA TTGCAATGAA 
GCCTTTGTCA AGATTAAGAT GGCTTTTCGA CCAGGCATGG TCGACTTACC AGAAGGGCAC 
AGAGAAGCTA ATGTTAATGC AATAACACTC CCAGAAGTTT TTCATGACTT TGATACAGCC 
CTGCCCGAAT TAAACGATAT TGATATTGAG GCTCAGTTTT CTATAAATCA GTCCAGAGCT 
GACGAGATAA CAATGAGAGA AGATTACGGT AGTTTGTCTC TTTCCTTACA AGATGACGGA 
TTTGGTGATA TAGGCTTTGA AGCAGAAACG CCTGAAATTA TACGATGCTC TATACCTTCA 
AATATTAATG ATAAAATATT TGATAATGAC GTTTTGGAAA ATATTGAGTC TCTGGATCCA 
CATTCATTGG ACGCCCATGC TGATATGCCT GGTTCTCGAT TGGATGGTGA TGGGTTTGGA 
GATTCTTTTG GACAACCAGC CCTTTTTGAA GACGATCTTT TTGGTGATCC TTCTCAACCA 
GTTGAGCAGA TTACAAAAGA ATCTACTACT GTTCTCAACG CAGATGATTC TGATGAAGAC 
GCCATTGATA ATATTCATAA TGTCCCTTCG CCTGCAACCT CGCTCGTTAA TTCGATTGAA 
GATGAGAAAG AGGAAAATAA TTTAAATGGA CATGCAAGTG TGTCCGAAAA TGTGCCAATG 
AATGAAATAA CGCTTGTTCA AAACGAGGAT GAGGGATTTG CGTTAGCTCC TTTAGATGTC 
TCAATGTATA AAGGCGTTAC AAAAGCCAAA CGCAAAAGAA AACTTATTAT TGACGAAATA 
AAAAATATTT CTGGAGAGGA AATGAAGGCT CAGTTAGCCG ACACATCTGA TATATTAACC 
ACACTTGATT TAGCCCCACC AACAAAAAGA TTAATGTATT GGAAAGAAAC TGGAGGTGTC 
GAAAAACTTT TTTCATTACC ATCGCGATCC ATACCAGCTA GAGCTTTGTT TGGTAATTAC 
AATAGACAGT TGTTTTCGCA TTCAACATTC TTTGAGGACT TCTCGAGTGT TGTGCCAATG 
GAAATTCTTG CACTGGAATT TTATACCAAA GAGAACGAGA ACGCATTAAT TATTTTTAAT 
AAAAAAGGTC GCAAACGTAA GAATGATAAT ATGTCCAATT TGTTTTTGGA CCATGTCCCC 
GATTCAGTTG TTCAAAGTTT GGAAGCTCCG GAAGTCCTGA GAGCGAATCA TAAATCTCTA 
GGGGTATCTA CCGTTTCTGT GGAGATTGTA TCAAAAGAGC AAGAAAGCAT TTCATGTCAA 
AATGAATTAA CATTTTTTGA TAATATGCGA AGTCCGGACC TCTTGTCCTT AAATGAAATG 
GAACAGTTCT CATCAATAAA TGAGCTACCT TTAACACCAA GAAACATGAA TCATGAAATG 
GGTGACGATT TTAATCAAGG AGACTCAACG CCAGCTGGGT TGGATCATGG TCATGCCACT 
CCGCAGCATG GAAATATTGG CGAGATGGAT CACGACTCTG TTATACCGAC AAAAAAGACT 
GCAGTCATTT TAAATGAATC TGTGGGTACA TCTGTACTAA GTGACAATGG CGTTTCAAAA 
AGGACAAATA ATATTTTAAA AGGATGGGAC AACTATGAAA TTCCCAGTTT CGTTGGCCAG 
AATCATGGTG ATGAACAAAT GGAAAATGAA ACCGATGAGC AATTTGAAGA ACGTGTTTTA 
AACAAGAGAG CCGCCCACCT TTTTATTGAC GTAAGAGCAC ATTTTATCGC CAAGGACAGC 
TTGGAACTTT CTCAGCTTAC GTCAGGAAAT TCCAGAAAAC AGGCGGCACA AAAATTTTAT 
TCCCTCCTCG TCTTAAAGAA GTTTAAGGTG CTTCACATTG ATCAATCTGC TCCCTATGCA 
GATATTACTA TTACTCGAGG ACCAACGTTC GAAAACCCAA AAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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vtd cDNA ORF clone, Drosophila melanogaster(fruit fly)

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