rl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	rl
Entrez Gene ID	3354888
Full Name	rolled
Synonyms	12559,BcDNA:RE08694,CG12559,CG18732,CT34260,CT39192,D-ERK,DERK,DERK-A,DmERK-A,DmERKA,DmErk,DmMAPK,Dmel\CG12559,DpERK,DpErk,Dsor2,E(sina)7,EC2-1,EK2-1,ERK,ERK-A,ERKA,ERKa,EY2-2,Erk,Erk/Map kinase,Erk1,Erk1/2,ErkA,GroupII,MAP-k,MAPK,MapK,Mapk,RL,Rl,SEM,SR2-1,Sem,Su(Raf)2B,dERK,dm-dpERK,dp-ERK,dpERK,dpERK1,dpERk,dpErk,dpMAPK,erk,l(2)41Ac,l(2R)EMS45-39,mapk,p-ERK,pERK,pMAPK,pMapK,rl/ERK,rl/MAPK,rll,sem
General protein information	Preferred Names rolled Names CG12559 gene product from transcript CG12559-RC CG12559-PA CG12559-PC CG12559-PD CG12559-PE CG12559-PG CG12559-PH CG12559-PI Diphospho-ERK ERK ERK/rolled Erk MAP kinase Extracellular-regulated kinase A Extracellular-signal-related kinase A MAP kinase MAP-kinase drosophila ERK enhancer of seven in absentia 7 extracellular signal-regulated kinase extracellular signal-regulated kinase A mitogen activated protein kinase mitogen-activated protein kinase phospho-ERK rl-PA rl-PC rl-PD rl-PE rl-PG rl-PH rl-PI roll sevenmaker
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: h41-h41\|2-55.1 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001110878.4	NP_001104348.2	rolled, isoform H
NM_001015122.3	NP_001015122.1	rolled, isoform C
NM_001300706.2	NP_001287635.1	rolled, isoform I
NM_001110879.2	NP_001104349.1	rolled, isoform E
NM_001110880.2	NP_001104350.1	rolled, isoform A
NM_001015121.3	NP_001015121.3	rolled, isoform G
NM_001015123.3	NP_001015123.1	rolled, isoform D

Pathways from BioSystems

Homologies

	MAPK1	XP_001089600.2
Bos taurus (cattle)	MAPK1	NP_786987.1
Gallus gallus (chicken)	MAPK1	NP_989481.1
Caenorhabditis elegans (roundworm)	mpk-1	NP_001022583.1
Oryza sativa (rice)	Os06g0154500	NP_001056846.1
Homo sapiens (human)	MAPK1	NP_002736.3
Canis lupus familiaris (dog)	MAPK1	NP_001104270.1
Rattus norvegicus (Norway rat)	Mapk1	NP_446294.1
Danio rerio (zebrafish)	mapk1	NP_878308.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009207	XP_319983.4
Xenopus tropicalis (tropical clawed frog)	mapk1	NP_001017127.1
Mus musculus (house mouse)	Mapk1	NP_001033752.1
Drosophila melanogaster (fruit fly)	rl	NP_001015122.1
Saccharomyces cerevisiae (baker's yeast)	KSS1	NP_011554.3
Eremothecium gossypii	AGOS_ACL191C	NP_983213.1
Arabidopsis thaliana (thale cress)	MPK6	NP_181907.1
Pan troglodytes (chimpanzee)	MAPK1	XP_515005.2
Kluyveromyces lactis	KLLA0A02497g	XP_451110.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In the presence of USP47, MAPK would be stabilized as USP47 acts to deubiquitinate MAPK, thus counteracting the direct ubiquitination of MAPK by POE/UBR4 activity.
Title: The Deubiquitinase USP47 Stabilizes MAPK by Counteracting the Function of the N-end Rule ligase POE/UBR4 in Drosophila.

PERK/ATF4 activated the JNK pathway through Rac1 and Slpr activation in apoptotic cells, leading to the expression of Dilp8.
Title: The PERK pathway independently triggers apoptosis and a Rac1/Slpr/JNK/Dilp8 signaling favoring tissue homeostasis in a chronic ER stress Drosophila model.

Corto, rl and dMP1 interact during wing tissue differentiation; Corto, ERK(rl) and dMP1 form a complex in the nucleus and co-localize on several sites on polytene chromosomes
Title: The MAP kinase ERK and its scaffold protein MP1 interact with the chromatin regulator Corto during Drosophila wing tissue development.

Holn1 acts downstream of EGFR and ERK signaling in the Grh/Fos mediated wound closure pathway.
Title: Hole-in-one mutant phenotypes link EGFR/ERK signaling to epithelial tissue repair in Drosophila.

These results identify a role for the Exon Junction Complex (EJC) in the splicing of a subset of transcripts and suggest that RAS/MAPK signaling depends on the regulation of MAPK levels by the EJC.
Title: The exon junction complex controls the splicing of MAPK and other long intron-containing transcripts in Drosophila.

The following rl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00006	NM_001015122.3 Latest version!	Drosophila melanogaster rolled (rl), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa00006	NM_001300706.2 Latest version!	Drosophila melanogaster rolled (rl), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa00006	NM_001015123.3 Latest version!	Drosophila melanogaster rolled (rl), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa00006	NM_001110879.2 Latest version!	Drosophila melanogaster rolled (rl), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa00007	NM_001110878.4 Latest version!	Drosophila melanogaster rolled (rl), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00008	NM_001015121.3 Latest version!	Drosophila melanogaster rolled (rl), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00009	NM_001110880.2 Latest version!	Drosophila melanogaster rolled (rl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rl ( NM_001015122.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001015122.1 Drosophila melanogaster rolled (rl), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00006
Clone ID Related Accession (Same CDS sequence)	NM_001015122.3 , NM_001300706.2 , NM_001110879.2 , NM_001015123.3
Accession Version	NM_001015122.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rolled, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001015122.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAT TTAATTCGAG CGGATCAGTA GTTAATGGTA CAGGATCTAC GGAAGTTCCT 
CAATCTAATG CTGAAGTTAT AAGGGGACAA ATATTTGAAG TTGGTCCTAG GTATATTAAA 
CTCGCCTATA TAGGTGAAGG AGCTTATGGC ATGGTTGTGT CTGCGGATGA CACGCTAACA 
AACCAAAGAG TTGCAATAAA AAAAATATCG CCCTTTGAAC ACCAAACTTA TTGTCAAAGG 
ACTCTCAGAG AAATAACCAT ATTGACCAGA TTTAAACATG AAAACATTAT TGATATTCGA 
GATATTCTTC GAGTTGATAG CATAGACCAA ATGAGAGATG TTTATATTGT ACAGTGTTTG 
ATGGAGACTG ATTTGTATAA ACTACTTAAA ACACAGAGGC TAAGTAATGA TCACATCTGT 
TACTTCTTAT ATCAGATATT GCGTGGACTC AAGTACATTC ATTCCGCAAA CGTCTTGCAT 
CGGGACCTTA AGCCAAGTAA TTTACTGTTG AACAAGACGT GCGACTTAAA AATTTGCGAC 
TTTGGATTGG CTCGTATTGC AGATCCCGAG CACGATCATA CTGGCTTTCT CACAGAATAC 
GTTGCTACCC GATGGTATAG AGCACCTGAA ATAATGCTTA ACTCAAAAGG ATACACCAAA 
TCTATAGACA TATGGTCCGT TGGCTGCATT TTGGCTGAAA TGTTAAGTAA TCGGCCAATA 
TTTCCTGGAA AACATTACCT GGATCAACTT AATCATATTC TTGGAGTATT GGGTTCACCG 
TCCCGGGACG ATTTAGAGTG TATTATTAAT GAAAAGGCAC GGAACTATTT GGAATCTTTA 
CCATTTAAGC CAAATGTACC CTGGGCGAAA CTATTTCCAA ATGCTGATGC GTTGGCTTTA 
GATCTCCTTG GAAAAATGTT AACATTTAAC CCGCATAAAC GGATTCCTGT CGAGGAAGCT 
CTTGCACATC CCTATTTAGA GCAATATTAT GATCCTGGAG ATGAGCCTGT CGCTGAAGTG 
CCATTTCGGA TTAATATGGA AAATGATGAC ATTTCTCGAG ATGCCCTGAA GTCGTTGATT 
TTTGAAGAAA CCTTAAAATT TAAGGAACGA CAACCAGACA ATGCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015122.1
CDS	127..1257
Translation	MEEFNSSGSVVNGTGSTEVPQSNAEVIRGQIFEVGPRYIKLAYIGEGAYGMVVSADDTLTNQRVAIKKISPFEHQTYCQRTLREITILTRFKHENIIDIRDILRVDSIDQMRDVYIVQCLMETDLYKLLKTQRLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNKTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGVLGSPSRDDLECIINEKARNYLESLPFKPNVPWAKLFPNADALALDLLGKMLTFNPHKRIPVEEALAHPYLEQYYDPGDEPVAEVPFRINMENDDISRDALKSLIFEETLKFKERQPDNAP

Target ORF information:

RefSeq Version	NM_001015122.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rolled (rl), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015122.3

ORF Insert Sequence:

ATGGAGGAAT TTAATTCGAG CGGATCAGTA GTTAATGGTA CAGGATCTAC GGAAGTTCCT 
CAATCTAATG CTGAAGTTAT AAGGGGACAA ATATTTGAAG TTGGTCCTAG GTATATTAAA 
CTCGCCTATA TAGGTGAAGG AGCTTATGGC ATGGTTGTGT CTGCGGATGA CACGCTAACA 
AACCAAAGAG TTGCAATAAA AAAAATATCG CCCTTTGAAC ACCAAACTTA TTGTCAAAGG 
ACTCTCAGAG AAATAACCAT ATTGACCAGA TTTAAACATG AAAACATTAT TGATATTCGA 
GATATTCTTC GAGTTGATAG CATAGACCAA ATGAGAGATG TTTATATTGT ACAGTGTTTG 
ATGGAGACTG ATTTGTATAA ACTACTTAAA ACACAGAGGC TAAGTAATGA TCACATCTGT 
TACTTCTTAT ATCAGATATT GCGTGGACTC AAGTACATTC ATTCCGCAAA CGTCTTGCAT 
CGGGACCTTA AGCCAAGTAA TTTACTGTTG AACAAGACGT GCGACTTAAA AATTTGCGAC 
TTTGGATTGG CTCGTATTGC AGATCCCGAG CACGATCATA CTGGCTTTCT CACAGAATAC 
GTTGCTACCC GATGGTATAG AGCACCTGAA ATAATGCTTA ACTCAAAAGG ATACACCAAA 
TCTATAGACA TATGGTCCGT TGGCTGCATT TTGGCTGAAA TGTTAAGTAA TCGGCCAATA 
TTTCCTGGAA AACATTACCT GGATCAACTT AATCATATTC TTGGAGTATT GGGTTCACCG 
TCCCGGGACG ATTTAGAGTG TATTATTAAT GAAAAGGCAC GGAACTATTT GGAATCTTTA 
CCATTTAAGC CAAATGTACC CTGGGCGAAA CTATTTCCAA ATGCTGATGC GTTGGCTTTA 
GATCTCCTTG GAAAAATGTT AACATTTAAC CCGCATAAAC GGATTCCTGT CGAGGAAGCT 
CTTGCACATC CCTATTTAGA GCAATATTAT GATCCTGGAG ATGAGCCTGT CGCTGAAGTG 
CCATTTCGGA TTAATATGGA AAATGATGAC ATTTCTCGAG ATGCCCTGAA GTCGTTGATT 
TTTGAAGAAA CCTTAAAATT TAAGGAACGA CAACCAGACA ATGCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rl ( NM_001300706.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287635.1 Drosophila melanogaster rolled (rl), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa00006
Clone ID Related Accession (Same CDS sequence)	NM_001015122.3 , NM_001300706.2 , NM_001110879.2 , NM_001015123.3
Accession Version	NM_001300706.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rolled, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Aug 4, 2014 this sequence version replaced NM_001300706.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAT TTAATTCGAG CGGATCAGTA GTTAATGGTA CAGGATCTAC GGAAGTTCCT 
CAATCTAATG CTGAAGTTAT AAGGGGACAA ATATTTGAAG TTGGTCCTAG GTATATTAAA 
CTCGCCTATA TAGGTGAAGG AGCTTATGGC ATGGTTGTGT CTGCGGATGA CACGCTAACA 
AACCAAAGAG TTGCAATAAA AAAAATATCG CCCTTTGAAC ACCAAACTTA TTGTCAAAGG 
ACTCTCAGAG AAATAACCAT ATTGACCAGA TTTAAACATG AAAACATTAT TGATATTCGA 
GATATTCTTC GAGTTGATAG CATAGACCAA ATGAGAGATG TTTATATTGT ACAGTGTTTG 
ATGGAGACTG ATTTGTATAA ACTACTTAAA ACACAGAGGC TAAGTAATGA TCACATCTGT 
TACTTCTTAT ATCAGATATT GCGTGGACTC AAGTACATTC ATTCCGCAAA CGTCTTGCAT 
CGGGACCTTA AGCCAAGTAA TTTACTGTTG AACAAGACGT GCGACTTAAA AATTTGCGAC 
TTTGGATTGG CTCGTATTGC AGATCCCGAG CACGATCATA CTGGCTTTCT CACAGAATAC 
GTTGCTACCC GATGGTATAG AGCACCTGAA ATAATGCTTA ACTCAAAAGG ATACACCAAA 
TCTATAGACA TATGGTCCGT TGGCTGCATT TTGGCTGAAA TGTTAAGTAA TCGGCCAATA 
TTTCCTGGAA AACATTACCT GGATCAACTT AATCATATTC TTGGAGTATT GGGTTCACCG 
TCCCGGGACG ATTTAGAGTG TATTATTAAT GAAAAGGCAC GGAACTATTT GGAATCTTTA 
CCATTTAAGC CAAATGTACC CTGGGCGAAA CTATTTCCAA ATGCTGATGC GTTGGCTTTA 
GATCTCCTTG GAAAAATGTT AACATTTAAC CCGCATAAAC GGATTCCTGT CGAGGAAGCT 
CTTGCACATC CCTATTTAGA GCAATATTAT GATCCTGGAG ATGAGCCTGT CGCTGAAGTG 
CCATTTCGGA TTAATATGGA AAATGATGAC ATTTCTCGAG ATGCCCTGAA GTCGTTGATT 
TTTGAAGAAA CCTTAAAATT TAAGGAACGA CAACCAGACA ATGCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287635.1
CDS	103..1233
Translation	MEEFNSSGSVVNGTGSTEVPQSNAEVIRGQIFEVGPRYIKLAYIGEGAYGMVVSADDTLTNQRVAIKKISPFEHQTYCQRTLREITILTRFKHENIIDIRDILRVDSIDQMRDVYIVQCLMETDLYKLLKTQRLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNKTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGVLGSPSRDDLECIINEKARNYLESLPFKPNVPWAKLFPNADALALDLLGKMLTFNPHKRIPVEEALAHPYLEQYYDPGDEPVAEVPFRINMENDDISRDALKSLIFEETLKFKERQPDNAP

Target ORF information:

RefSeq Version	NM_001300706.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rolled (rl), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300706.2

ORF Insert Sequence:

ATGGAGGAAT TTAATTCGAG CGGATCAGTA GTTAATGGTA CAGGATCTAC GGAAGTTCCT 
CAATCTAATG CTGAAGTTAT AAGGGGACAA ATATTTGAAG TTGGTCCTAG GTATATTAAA 
CTCGCCTATA TAGGTGAAGG AGCTTATGGC ATGGTTGTGT CTGCGGATGA CACGCTAACA 
AACCAAAGAG TTGCAATAAA AAAAATATCG CCCTTTGAAC ACCAAACTTA TTGTCAAAGG 
ACTCTCAGAG AAATAACCAT ATTGACCAGA TTTAAACATG AAAACATTAT TGATATTCGA 
GATATTCTTC GAGTTGATAG CATAGACCAA ATGAGAGATG TTTATATTGT ACAGTGTTTG 
ATGGAGACTG ATTTGTATAA ACTACTTAAA ACACAGAGGC TAAGTAATGA TCACATCTGT 
TACTTCTTAT ATCAGATATT GCGTGGACTC AAGTACATTC ATTCCGCAAA CGTCTTGCAT 
CGGGACCTTA AGCCAAGTAA TTTACTGTTG AACAAGACGT GCGACTTAAA AATTTGCGAC 
TTTGGATTGG CTCGTATTGC AGATCCCGAG CACGATCATA CTGGCTTTCT CACAGAATAC 
GTTGCTACCC GATGGTATAG AGCACCTGAA ATAATGCTTA ACTCAAAAGG ATACACCAAA 
TCTATAGACA TATGGTCCGT TGGCTGCATT TTGGCTGAAA TGTTAAGTAA TCGGCCAATA 
TTTCCTGGAA AACATTACCT GGATCAACTT AATCATATTC TTGGAGTATT GGGTTCACCG 
TCCCGGGACG ATTTAGAGTG TATTATTAAT GAAAAGGCAC GGAACTATTT GGAATCTTTA 
CCATTTAAGC CAAATGTACC CTGGGCGAAA CTATTTCCAA ATGCTGATGC GTTGGCTTTA 
GATCTCCTTG GAAAAATGTT AACATTTAAC CCGCATAAAC GGATTCCTGT CGAGGAAGCT 
CTTGCACATC CCTATTTAGA GCAATATTAT GATCCTGGAG ATGAGCCTGT CGCTGAAGTG 
CCATTTCGGA TTAATATGGA AAATGATGAC ATTTCTCGAG ATGCCCTGAA GTCGTTGATT 
TTTGAAGAAA CCTTAAAATT TAAGGAACGA CAACCAGACA ATGCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rl ( NM_001110879.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001104349.1 Drosophila melanogaster rolled (rl), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa00006
Clone ID Related Accession (Same CDS sequence)	NM_001015122.3 , NM_001300706.2 , NM_001110879.2 , NM_001015123.3
Accession Version	NM_001110879.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rolled, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001110879.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAT TTAATTCGAG CGGATCAGTA GTTAATGGTA CAGGATCTAC GGAAGTTCCT 
CAATCTAATG CTGAAGTTAT AAGGGGACAA ATATTTGAAG TTGGTCCTAG GTATATTAAA 
CTCGCCTATA TAGGTGAAGG AGCTTATGGC ATGGTTGTGT CTGCGGATGA CACGCTAACA 
AACCAAAGAG TTGCAATAAA AAAAATATCG CCCTTTGAAC ACCAAACTTA TTGTCAAAGG 
ACTCTCAGAG AAATAACCAT ATTGACCAGA TTTAAACATG AAAACATTAT TGATATTCGA 
GATATTCTTC GAGTTGATAG CATAGACCAA ATGAGAGATG TTTATATTGT ACAGTGTTTG 
ATGGAGACTG ATTTGTATAA ACTACTTAAA ACACAGAGGC TAAGTAATGA TCACATCTGT 
TACTTCTTAT ATCAGATATT GCGTGGACTC AAGTACATTC ATTCCGCAAA CGTCTTGCAT 
CGGGACCTTA AGCCAAGTAA TTTACTGTTG AACAAGACGT GCGACTTAAA AATTTGCGAC 
TTTGGATTGG CTCGTATTGC AGATCCCGAG CACGATCATA CTGGCTTTCT CACAGAATAC 
GTTGCTACCC GATGGTATAG AGCACCTGAA ATAATGCTTA ACTCAAAAGG ATACACCAAA 
TCTATAGACA TATGGTCCGT TGGCTGCATT TTGGCTGAAA TGTTAAGTAA TCGGCCAATA 
TTTCCTGGAA AACATTACCT GGATCAACTT AATCATATTC TTGGAGTATT GGGTTCACCG 
TCCCGGGACG ATTTAGAGTG TATTATTAAT GAAAAGGCAC GGAACTATTT GGAATCTTTA 
CCATTTAAGC CAAATGTACC CTGGGCGAAA CTATTTCCAA ATGCTGATGC GTTGGCTTTA 
GATCTCCTTG GAAAAATGTT AACATTTAAC CCGCATAAAC GGATTCCTGT CGAGGAAGCT 
CTTGCACATC CCTATTTAGA GCAATATTAT GATCCTGGAG ATGAGCCTGT CGCTGAAGTG 
CCATTTCGGA TTAATATGGA AAATGATGAC ATTTCTCGAG ATGCCCTGAA GTCGTTGATT 
TTTGAAGAAA CCTTAAAATT TAAGGAACGA CAACCAGACA ATGCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104349.1
CDS	86..1216
Translation	MEEFNSSGSVVNGTGSTEVPQSNAEVIRGQIFEVGPRYIKLAYIGEGAYGMVVSADDTLTNQRVAIKKISPFEHQTYCQRTLREITILTRFKHENIIDIRDILRVDSIDQMRDVYIVQCLMETDLYKLLKTQRLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNKTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGVLGSPSRDDLECIINEKARNYLESLPFKPNVPWAKLFPNADALALDLLGKMLTFNPHKRIPVEEALAHPYLEQYYDPGDEPVAEVPFRINMENDDISRDALKSLIFEETLKFKERQPDNAP

Target ORF information:

RefSeq Version	NM_001110879.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rolled (rl), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110879.2

ORF Insert Sequence:

ATGGAGGAAT TTAATTCGAG CGGATCAGTA GTTAATGGTA CAGGATCTAC GGAAGTTCCT 
CAATCTAATG CTGAAGTTAT AAGGGGACAA ATATTTGAAG TTGGTCCTAG GTATATTAAA 
CTCGCCTATA TAGGTGAAGG AGCTTATGGC ATGGTTGTGT CTGCGGATGA CACGCTAACA 
AACCAAAGAG TTGCAATAAA AAAAATATCG CCCTTTGAAC ACCAAACTTA TTGTCAAAGG 
ACTCTCAGAG AAATAACCAT ATTGACCAGA TTTAAACATG AAAACATTAT TGATATTCGA 
GATATTCTTC GAGTTGATAG CATAGACCAA ATGAGAGATG TTTATATTGT ACAGTGTTTG 
ATGGAGACTG ATTTGTATAA ACTACTTAAA ACACAGAGGC TAAGTAATGA TCACATCTGT 
TACTTCTTAT ATCAGATATT GCGTGGACTC AAGTACATTC ATTCCGCAAA CGTCTTGCAT 
CGGGACCTTA AGCCAAGTAA TTTACTGTTG AACAAGACGT GCGACTTAAA AATTTGCGAC 
TTTGGATTGG CTCGTATTGC AGATCCCGAG CACGATCATA CTGGCTTTCT CACAGAATAC 
GTTGCTACCC GATGGTATAG AGCACCTGAA ATAATGCTTA ACTCAAAAGG ATACACCAAA 
TCTATAGACA TATGGTCCGT TGGCTGCATT TTGGCTGAAA TGTTAAGTAA TCGGCCAATA 
TTTCCTGGAA AACATTACCT GGATCAACTT AATCATATTC TTGGAGTATT GGGTTCACCG 
TCCCGGGACG ATTTAGAGTG TATTATTAAT GAAAAGGCAC GGAACTATTT GGAATCTTTA 
CCATTTAAGC CAAATGTACC CTGGGCGAAA CTATTTCCAA ATGCTGATGC GTTGGCTTTA 
GATCTCCTTG GAAAAATGTT AACATTTAAC CCGCATAAAC GGATTCCTGT CGAGGAAGCT 
CTTGCACATC CCTATTTAGA GCAATATTAT GATCCTGGAG ATGAGCCTGT CGCTGAAGTG 
CCATTTCGGA TTAATATGGA AAATGATGAC ATTTCTCGAG ATGCCCTGAA GTCGTTGATT 
TTTGAAGAAA CCTTAAAATT TAAGGAACGA CAACCAGACA ATGCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rl ( NM_001015123.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001015123.1 Drosophila melanogaster rolled (rl), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa00006
Clone ID Related Accession (Same CDS sequence)	NM_001015122.3 , NM_001300706.2 , NM_001110879.2 , NM_001015123.3
Accession Version	NM_001015123.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rolled, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001015123.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAT TTAATTCGAG CGGATCAGTA GTTAATGGTA CAGGATCTAC GGAAGTTCCT 
CAATCTAATG CTGAAGTTAT AAGGGGACAA ATATTTGAAG TTGGTCCTAG GTATATTAAA 
CTCGCCTATA TAGGTGAAGG AGCTTATGGC ATGGTTGTGT CTGCGGATGA CACGCTAACA 
AACCAAAGAG TTGCAATAAA AAAAATATCG CCCTTTGAAC ACCAAACTTA TTGTCAAAGG 
ACTCTCAGAG AAATAACCAT ATTGACCAGA TTTAAACATG AAAACATTAT TGATATTCGA 
GATATTCTTC GAGTTGATAG CATAGACCAA ATGAGAGATG TTTATATTGT ACAGTGTTTG 
ATGGAGACTG ATTTGTATAA ACTACTTAAA ACACAGAGGC TAAGTAATGA TCACATCTGT 
TACTTCTTAT ATCAGATATT GCGTGGACTC AAGTACATTC ATTCCGCAAA CGTCTTGCAT 
CGGGACCTTA AGCCAAGTAA TTTACTGTTG AACAAGACGT GCGACTTAAA AATTTGCGAC 
TTTGGATTGG CTCGTATTGC AGATCCCGAG CACGATCATA CTGGCTTTCT CACAGAATAC 
GTTGCTACCC GATGGTATAG AGCACCTGAA ATAATGCTTA ACTCAAAAGG ATACACCAAA 
TCTATAGACA TATGGTCCGT TGGCTGCATT TTGGCTGAAA TGTTAAGTAA TCGGCCAATA 
TTTCCTGGAA AACATTACCT GGATCAACTT AATCATATTC TTGGAGTATT GGGTTCACCG 
TCCCGGGACG ATTTAGAGTG TATTATTAAT GAAAAGGCAC GGAACTATTT GGAATCTTTA 
CCATTTAAGC CAAATGTACC CTGGGCGAAA CTATTTCCAA ATGCTGATGC GTTGGCTTTA 
GATCTCCTTG GAAAAATGTT AACATTTAAC CCGCATAAAC GGATTCCTGT CGAGGAAGCT 
CTTGCACATC CCTATTTAGA GCAATATTAT GATCCTGGAG ATGAGCCTGT CGCTGAAGTG 
CCATTTCGGA TTAATATGGA AAATGATGAC ATTTCTCGAG ATGCCCTGAA GTCGTTGATT 
TTTGAAGAAA CCTTAAAATT TAAGGAACGA CAACCAGACA ATGCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015123.1
CDS	308..1438
Translation	MEEFNSSGSVVNGTGSTEVPQSNAEVIRGQIFEVGPRYIKLAYIGEGAYGMVVSADDTLTNQRVAIKKISPFEHQTYCQRTLREITILTRFKHENIIDIRDILRVDSIDQMRDVYIVQCLMETDLYKLLKTQRLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNKTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGVLGSPSRDDLECIINEKARNYLESLPFKPNVPWAKLFPNADALALDLLGKMLTFNPHKRIPVEEALAHPYLEQYYDPGDEPVAEVPFRINMENDDISRDALKSLIFEETLKFKERQPDNAP

Target ORF information:

RefSeq Version	NM_001015123.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rolled (rl), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015123.3

ORF Insert Sequence:

ATGGAGGAAT TTAATTCGAG CGGATCAGTA GTTAATGGTA CAGGATCTAC GGAAGTTCCT 
CAATCTAATG CTGAAGTTAT AAGGGGACAA ATATTTGAAG TTGGTCCTAG GTATATTAAA 
CTCGCCTATA TAGGTGAAGG AGCTTATGGC ATGGTTGTGT CTGCGGATGA CACGCTAACA 
AACCAAAGAG TTGCAATAAA AAAAATATCG CCCTTTGAAC ACCAAACTTA TTGTCAAAGG 
ACTCTCAGAG AAATAACCAT ATTGACCAGA TTTAAACATG AAAACATTAT TGATATTCGA 
GATATTCTTC GAGTTGATAG CATAGACCAA ATGAGAGATG TTTATATTGT ACAGTGTTTG 
ATGGAGACTG ATTTGTATAA ACTACTTAAA ACACAGAGGC TAAGTAATGA TCACATCTGT 
TACTTCTTAT ATCAGATATT GCGTGGACTC AAGTACATTC ATTCCGCAAA CGTCTTGCAT 
CGGGACCTTA AGCCAAGTAA TTTACTGTTG AACAAGACGT GCGACTTAAA AATTTGCGAC 
TTTGGATTGG CTCGTATTGC AGATCCCGAG CACGATCATA CTGGCTTTCT CACAGAATAC 
GTTGCTACCC GATGGTATAG AGCACCTGAA ATAATGCTTA ACTCAAAAGG ATACACCAAA 
TCTATAGACA TATGGTCCGT TGGCTGCATT TTGGCTGAAA TGTTAAGTAA TCGGCCAATA 
TTTCCTGGAA AACATTACCT GGATCAACTT AATCATATTC TTGGAGTATT GGGTTCACCG 
TCCCGGGACG ATTTAGAGTG TATTATTAAT GAAAAGGCAC GGAACTATTT GGAATCTTTA 
CCATTTAAGC CAAATGTACC CTGGGCGAAA CTATTTCCAA ATGCTGATGC GTTGGCTTTA 
GATCTCCTTG GAAAAATGTT AACATTTAAC CCGCATAAAC GGATTCCTGT CGAGGAAGCT 
CTTGCACATC CCTATTTAGA GCAATATTAT GATCCTGGAG ATGAGCCTGT CGCTGAAGTG 
CCATTTCGGA TTAATATGGA AAATGATGAC ATTTCTCGAG ATGCCCTGAA GTCGTTGATT 
TTTGAAGAAA CCTTAAAATT TAAGGAACGA CAACCAGACA ATGCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rl ( NM_001110878.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001104348.2 Drosophila melanogaster rolled (rl), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa00007
Clone ID Related Accession (Same CDS sequence)	NM_001110878.4
Accession Version	NM_001110878.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rolled, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Aug 4, 2014 this sequence version replaced NM_001110878.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAGATG TTTATATTGT ACAGTGTTTG ATGGAGACTG ATTTGTATAA ACTACTTAAA 
ACACAGAGGC TAAGTAATGA TCACATCTGT TACTTCTTAT ATCAGATATT GCGTGGACTC 
AAGTACATTC ATTCCGCAAA CGTCTTGCAT CGGGACCTTA AGCCAAGTAA TTTACTGTTG 
AACAAGACGT GCGACTTAAA AATTTGCGAC TTTGGATTGG CTCGTATTGC AGATCCCGAG 
CACGATCATA CTGGCTTTCT CACAGAATAC GTTGCTACCC GATGGTATAG AGCACCTGAA 
ATAATGCTTA ACTCAAAAGG ATACACCAAA TCTATAGACA TATGGTCCGT TGGCTGCATT 
TTGGCTGAAA TGTTAAGTAA TCGGCCAATA TTTCCTGGAA AACATTACCT GGATCAACTT 
AATCATATTC TTGGAGTATT GGGTTCACCG TCCCGGGACG ATTTAGAGTG TATTATTAAT 
GAAAAGGCAC GGAACTATTT GGAATCTTTA CCATTTAAGC CAAATGTACC CTGGGCGAAA 
CTATTTCCAA ATGCTGATGC GTTGGCTTTA GATCTCCTTG GAAAAATGTT AACATTTAAC 
CCGCATAAAC GGATTCCTGT CGAGGAAGCT CTTGCACATC CCTATTTAGA GCAATATTAT 
GATCCTGGAG ATGAGCCTGT CGCTGAAGTG CCATTTCGGA TTAATATGGA AAATGATGAC 
ATTTCTCGAG ATGCCCTGAA GTCGTTGATT TTTGAAGAAA CCTTAAAATT TAAGGAACGA 
CAACCAGACA ATGCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104348.2
CDS	495..1295
Translation	MRDVYIVQCLMETDLYKLLKTQRLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNKTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGVLGSPSRDDLECIINEKARNYLESLPFKPNVPWAKLFPNADALALDLLGKMLTFNPHKRIPVEEALAHPYLEQYYDPGDEPVAEVPFRINMENDDISRDALKSLIFEETLKFKERQPDNAP

Target ORF information:

RefSeq Version	NM_001110878.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rolled (rl), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110878.4

ORF Insert Sequence:

ATGAGAGATG TTTATATTGT ACAGTGTTTG ATGGAGACTG ATTTGTATAA ACTACTTAAA 
ACACAGAGGC TAAGTAATGA TCACATCTGT TACTTCTTAT ATCAGATATT GCGTGGACTC 
AAGTACATTC ATTCCGCAAA CGTCTTGCAT CGGGACCTTA AGCCAAGTAA TTTACTGTTG 
AACAAGACGT GCGACTTAAA AATTTGCGAC TTTGGATTGG CTCGTATTGC AGATCCCGAG 
CACGATCATA CTGGCTTTCT CACAGAATAC GTTGCTACCC GATGGTATAG AGCACCTGAA 
ATAATGCTTA ACTCAAAAGG ATACACCAAA TCTATAGACA TATGGTCCGT TGGCTGCATT 
TTGGCTGAAA TGTTAAGTAA TCGGCCAATA TTTCCTGGAA AACATTACCT GGATCAACTT 
AATCATATTC TTGGAGTATT GGGTTCACCG TCCCGGGACG ATTTAGAGTG TATTATTAAT 
GAAAAGGCAC GGAACTATTT GGAATCTTTA CCATTTAAGC CAAATGTACC CTGGGCGAAA 
CTATTTCCAA ATGCTGATGC GTTGGCTTTA GATCTCCTTG GAAAAATGTT AACATTTAAC 
CCGCATAAAC GGATTCCTGT CGAGGAAGCT CTTGCACATC CCTATTTAGA GCAATATTAT 
GATCCTGGAG ATGAGCCTGT CGCTGAAGTG CCATTTCGGA TTAATATGGA AAATGATGAC 
ATTTCTCGAG ATGCCCTGAA GTCGTTGATT TTTGAAGAAA CCTTAAAATT TAAGGAACGA 
CAACCAGACA ATGCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rl ( NM_001015121.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001015121.3 Drosophila melanogaster rolled (rl), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa00008
Clone ID Related Accession (Same CDS sequence)	NM_001015121.3
Accession Version	NM_001015121.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rolled, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001015121.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCTG CCTTAGTTAT TAGGTCTGCG GATGACACGC TAACAAACCA AAGAGTTGCA 
ATAAAAAAAA TATCGCCCTT TGAACACCAA ACTTATTGTC AAAGGACTCT CAGAGAAATA 
ACCATATTGA CCAGATTTAA ACATGAAAAC ATTATTGATA TTCGAGATAT TCTTCGAGTT 
GATAGCATAG ACCAAATGAG AGATGTTTAT ATTGTACAGT GTTTGATGGA GACTGATTTG 
TATAAACTAC TTAAAACACA GAGGCTAAGT AATGATCACA TCTGTTACTT CTTATATCAG 
ATATTGCGTG GACTCAAGTA CATTCATTCC GCAAACGTCT TGCATCGGGA CCTTAAGCCA 
AGTAATTTAC TGTTGAACAA GACGTGCGAC TTAAAAATTT GCGACTTTGG ATTGGCTCGT 
ATTGCAGATC CCGAGCACGA TCATACTGGC TTTCTCACAG AATACGTTGC TACCCGATGG 
TATAGAGCAC CTGAAATAAT GCTTAACTCA AAAGGATACA CCAAATCTAT AGACATATGG 
TCCGTTGGCT GCATTTTGGC TGAAATGTTA AGTAATCGGC CAATATTTCC TGGAAAACAT 
TACCTGGATC AACTTAATCA TATTCTTGGA GTATTGGGTT CACCGTCCCG GGACGATTTA 
GAGTGTATTA TTAATGAAAA GGCACGGAAC TATTTGGAAT CTTTACCATT TAAGCCAAAT 
GTACCCTGGG CGAAACTATT TCCAAATGCT GATGCGTTGG CTTTAGATCT CCTTGGAAAA 
ATGTTAACAT TTAACCCGCA TAAACGGATT CCTGTCGAGG AAGCTCTTGC ACATCCCTAT 
TTAGAGCAAT ATTATGATCC TGGAGATGAG CCTGTCGCTG AAGTGCCATT TCGGATTAAT 
ATGGAAAATG ATGACATTTC TCGAGATGCC CTGAAGTCGT TGATTTTTGA AGAAACCTTA 
AAATTTAAGG AACGACAACC AGACAATGCG CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001015121.3
CDS	331..1326
Translation	MESALVIRSADDTLTNQRVAIKKISPFEHQTYCQRTLREITILTRFKHENIIDIRDILRVDSIDQMRDVYIVQCLMETDLYKLLKTQRLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNKTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGVLGSPSRDDLECIINEKARNYLESLPFKPNVPWAKLFPNADALALDLLGKMLTFNPHKRIPVEEALAHPYLEQYYDPGDEPVAEVPFRINMENDDISRDALKSLIFEETLKFKERQPDNAP

Target ORF information:

RefSeq Version	NM_001015121.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rolled (rl), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001015121.3

ORF Insert Sequence:

ATGGAGTCTG CCTTAGTTAT TAGGTCTGCG GATGACACGC TAACAAACCA AAGAGTTGCA 
ATAAAAAAAA TATCGCCCTT TGAACACCAA ACTTATTGTC AAAGGACTCT CAGAGAAATA 
ACCATATTGA CCAGATTTAA ACATGAAAAC ATTATTGATA TTCGAGATAT TCTTCGAGTT 
GATAGCATAG ACCAAATGAG AGATGTTTAT ATTGTACAGT GTTTGATGGA GACTGATTTG 
TATAAACTAC TTAAAACACA GAGGCTAAGT AATGATCACA TCTGTTACTT CTTATATCAG 
ATATTGCGTG GACTCAAGTA CATTCATTCC GCAAACGTCT TGCATCGGGA CCTTAAGCCA 
AGTAATTTAC TGTTGAACAA GACGTGCGAC TTAAAAATTT GCGACTTTGG ATTGGCTCGT 
ATTGCAGATC CCGAGCACGA TCATACTGGC TTTCTCACAG AATACGTTGC TACCCGATGG 
TATAGAGCAC CTGAAATAAT GCTTAACTCA AAAGGATACA CCAAATCTAT AGACATATGG 
TCCGTTGGCT GCATTTTGGC TGAAATGTTA AGTAATCGGC CAATATTTCC TGGAAAACAT 
TACCTGGATC AACTTAATCA TATTCTTGGA GTATTGGGTT CACCGTCCCG GGACGATTTA 
GAGTGTATTA TTAATGAAAA GGCACGGAAC TATTTGGAAT CTTTACCATT TAAGCCAAAT 
GTACCCTGGG CGAAACTATT TCCAAATGCT GATGCGTTGG CTTTAGATCT CCTTGGAAAA 
ATGTTAACAT TTAACCCGCA TAAACGGATT CCTGTCGAGG AAGCTCTTGC ACATCCCTAT 
TTAGAGCAAT ATTATGATCC TGGAGATGAG CCTGTCGCTG AAGTGCCATT TCGGATTAAT 
ATGGAAAATG ATGACATTTC TCGAGATGCC CTGAAGTCGT TGATTTTTGA AGAAACCTTA 
AAATTTAAGG AACGACAACC AGACAATGCG CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rl ( NM_001110880.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001104350.1 Drosophila melanogaster rolled (rl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00009
Clone ID Related Accession (Same CDS sequence)	NM_001110880.2
Accession Version	NM_001110880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rolled, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001110880.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAT TTAATTCGAG CGGATCAGTA GTTAATGGTA CAGGATCTAC GGAAGTTCCT 
CAATCTAATG CTGAAGTTAT AAGGGGACAA ATATTTGAAG TTGGTCCTAG GTATATTAAA 
CTCGCCTATA TAGGTGAAGG AGCTTATGGC ATGGTTGTGT CTGCGGATGA CACGCTAACA 
AACCAAAGAG TTGCAATAAA AAAAATATCG CCCTTTGAAC ACCAAACTTA TTGTCAAAGG 
ACTCTCAGAG AAATAACCAT ATTGACCAGA TTTAAACATG AAAACATTAT TGATATTCGA 
GATATTCTTC GAGTTGATAG CATAGACCAA ATGAGAGATG TTTATATTGT ACAGTGTTTG 
ATGGAGACTG ATTTGTATAA ACTACTTAAA ACACAGGTAA TGGTGCTCCC TGATATTTGG 
TTAAAATTTA TTGAAAGAAT AACTACCATT GATATGTGGT GTATTATCTT GGCCATTACT 
CCAAATACCA AGACTGAGTA CAATTGTAGG TTGTCGGACA TTTTCAATTA TGGAAGTGAT 
TCGCGTTATG CTTTCATGCG GGCTTTAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104350.1
CDS	308..880
Translation	MEEFNSSGSVVNGTGSTEVPQSNAEVIRGQIFEVGPRYIKLAYIGEGAYGMVVSADDTLTNQRVAIKKISPFEHQTYCQRTLREITILTRFKHENIIDIRDILRVDSIDQMRDVYIVQCLMETDLYKLLKTQVMVLPDIWLKFIERITTIDMWCIILAITPNTKTEYNCRLSDIFNYGSDSRYAFMRALK

Target ORF information:

RefSeq Version	NM_001110880.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rolled (rl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110880.2

ORF Insert Sequence:

ATGGAGGAAT TTAATTCGAG CGGATCAGTA GTTAATGGTA CAGGATCTAC GGAAGTTCCT 
CAATCTAATG CTGAAGTTAT AAGGGGACAA ATATTTGAAG TTGGTCCTAG GTATATTAAA 
CTCGCCTATA TAGGTGAAGG AGCTTATGGC ATGGTTGTGT CTGCGGATGA CACGCTAACA 
AACCAAAGAG TTGCAATAAA AAAAATATCG CCCTTTGAAC ACCAAACTTA TTGTCAAAGG 
ACTCTCAGAG AAATAACCAT ATTGACCAGA TTTAAACATG AAAACATTAT TGATATTCGA 
GATATTCTTC GAGTTGATAG CATAGACCAA ATGAGAGATG TTTATATTGT ACAGTGTTTG 
ATGGAGACTG ATTTGTATAA ACTACTTAAA ACACAGGTAA TGGTGCTCCC TGATATTTGG 
TTAAAATTTA TTGAAAGAAT AACTACCATT GATATGTGGT GTATTATCTT GGCCATTACT 
CCAAATACCA AGACTGAGTA CAATTGTAGG TTGTCGGACA TTTTCAATTA TGGAAGTGAT 
TCGCGTTATG CTTTCATGCG GGCTTTAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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rl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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