Mad cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mad
Entrez Gene ID	33529
Full Name	Mothers against dpp
Synonyms	2/23,CG12399,Dmel\CG12399,E(zen)2,En(vvl),MAD,Mat,P-Mad,P-mad,PMad,Smad,Smad1,apg,c28,dMAD,dMad,l(2)K00237,l(2)k00237,mad,p-Mad,pMAD,pMad,pSmad
General protein information	Preferred Names Mothers against dpp Names CG12399 gene product from transcript CG12399-RB CG12399-PA CG12399-PB Mad-PA Mad-PB Mothers-against-decapentaplegic apang mother against Dpp mother against decapentaplegic mothers against decapentaplegi mothers against decapentaplegic mothers against dpp phospho-Smad phosphoSmad phosphorylated smad
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23D3-23D3\|2-7.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273063.1	NP_001259992.1	mothers against dpp, isoform B
NM_057669.3	NP_477017.1	mothers against dpp, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SMAD1	XP_001148403.1
Xenopus tropicalis (tropical clawed frog)	smad1	NP_001007481.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008551	XP_314661.3
Homo sapiens (human)	SMAD1	NP_001003688.1
Bos taurus (cattle)	SMAD1	NP_001069691.2
Gallus gallus (chicken)	SMAD1	NP_001188384.1
Canis lupus familiaris (dog)	SMAD1	XP_005629368.1
Rattus norvegicus (Norway rat)	Smad1	NP_037262.2
	SMAD1	XP_001096679.1
Mus musculus (house mouse)	Smad1	NP_032565.2
Danio rerio (zebrafish)	smad1	NP_571431.1
Drosophila melanogaster (fruit fly)	Mad	NP_477017.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This report provides a genetic analysis of primary nociceptive neuron mechanisms that promote sensitization in response to injury. Drosophila melanogaster larvae whose primary nociceptive neurons were reduced in levels of specific components of the BMP signaling pathway, were injured and then tested for nocifensive responses to a normally subnoxious stimulus.

LTR sequence of the MDG4 retrotransposon contains the MAD protein binding site that affects the east-dependent repression
Title: LTR sequence of the MDG4 retrotransposon contains the MAD protein binding site that affects the east-dependent repression.

we identify key roles for the Zelda and Zerknullt transcription factors in establishing the resulting expression domain, and find widespread binding of insulator proteins to the Mad and Brinker-bound genomic regions. Analysis of embryos lacking the BEAF-32 insulator protein shows reduced transcription of a peak BMP target gene and a reduction in the number of amnioserosa cells, the fate specified by peak BMP signaling.
Title: Regulation of the BMP Signaling-Responsive Transcriptional Network in the Drosophila Embryo.

Mad linker phosphorylations control the intensity and range of the BMP-activity gradient in developing Drosophila tissues.
Title: Mad linker phosphorylations control the intensity and range of the BMP-activity gradient in developing Drosophila tissues.

During development, synaptic pMad accumulation followed the arrival and clustering of ionotropic glutamate receptors at neuromuscular junction synapses.
Title: Postsynaptic glutamate receptors regulate local BMP signaling at the Drosophila neuromuscular junction.

The following Mad gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mad cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13610	NM_057669.3 Latest version!	Drosophila melanogaster mothers against dpp, transcript variant A (Mad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa16145	NM_001273063.1 Latest version!	Drosophila melanogaster mothers against dpp, transcript variant B (Mad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mad ( NM_057669.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477017.1 Drosophila melanogaster mothers against dpp, transcript variant A (Mad), mRNA.

Price & Availability↑

CloneID	OFa13610
Clone ID Related Accession (Same CDS sequence)	NM_057669.3
Accession Version	NM_057669.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mothers against dpp, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057669.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACACCG ACGATGTGGA ATCGAACACA AGCAGCGCGA TGTCCACACT GGGCTCGCTA 
TTCTCCTTCA CATCGCCGGC GGTGAAGAAG CTGCTGGGCT GGAAACAGGG TGACGAAGAG 
GAGAAGTGGG CGGAGAAGGC CGTCGACAGT CTGGTGAAGA AGTTGAAGAA GCGCAAGGGC 
GCCATCGAGG AGCTGGAGCG TGCGCTCTCC TGTCCCGGTC AGCCCTCGAA GTGTGTCACC 
ATTCCACGCT CGCTGGACGG ACGATTACAG GTCTCCCATC GCAAGGGTCT GCCGCATGTG 
ATCTACTGCC GCGTGTGGCG CTGGCCAGAT CTGCAGTCGC ACCACGAACT GAAGCCACTC 
GAGCTGTGCC AGTATCCGTT CAGCGCCAAG CAGAAGGAGG TGTGCATCAA TCCGTATCAC 
TATAAGCGCG TGGAGAGTCC GGTGCTCCCG CCAGTACTCG TTCCTCGCCA CTCGGAATTC 
GCGCCCGGTC ACTCGATGCT GCAGTTCAAC CATGTGGCCG AGCCCAGTAT GCCGCACAAT 
GTGAGCTACT CGAACAGTGG ATTCAACTCG CACAGCTTGA GCACCAGCAA CACATCGGTG 
GGCAGTCCGA GTTCCGTCAA CTCCAATCCC AATTCGCCGT ACGACAGCTT GGCGGGAACA 
CCGCCACCCG CCTACAGTCC CTCGGAGGAT GGCAACTCCA ACAATCCGAA CGACGGTGGC 
CAGCTGCTGG ATGCTCAGAT GGGCGATGTT GCCCAGGTTA GCTATTCGGA GCCCGCCTTC 
TGGGCGTCGA TAGCCTACTA CGAGCTAAAT TGCCGCGTGG GCGAGGTGTT CCACTGCAAC 
AACAACTCCG TGATCGTTGA CGGTTTCACG AATCCTTCCA ACAACTCGGA CCGCTGCTGT 
CTCGGCCAGC TGAGCAACGT GAACAGGAAT AGCACCATCG AGAACACACG CCGTCATATA 
GGCAAGGGTG TTCATTTATA CTATGTGACC GGCGAGGTCT ACGCCGAATG CCTATCCGAC 
TCCGCCATTT TCGTGCAGTC GCGCAACTGC AACTACCACC ACGGATTCCA TCCGAGCACC 
GTGTGCAAAA TACCGCCGGG CTGCTCGCTG AAGATCTTCA ACAATCAGGA ATTTGCTCAG 
CTGCTGTCGC AGTCGGTGAA CAATGGATTC GAGGCCGTCT ACGAGCTGAC AAAGATGTGC 
ACGATCCGGA TGTCGTTCGT GAAGGGCTGG GGTGCGGAGT ATCATCGCCA GGATGTGACC 
TCGACGCCCT GTTGGATTGA AATCCATCTG CACGGGCCGC TCCAGTGGCT GGACAAGGTG 
CTCACCCAAA TGGGCTCTCC GCATAATGCA ATTAGTTCGG TATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477017.1
CDS	357..1724
Translation	MDTDDVESNTSSAMSTLGSLFSFTSPAVKKLLGWKQGDEEEKWAEKAVDSLVKKLKKRKGAIEELERALSCPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLELCQYPFSAKQKEVCINPYHYKRVESPVLPPVLVPRHSEFAPGHSMLQFNHVAEPSMPHNVSYSNSGFNSHSLSTSNTSVGSPSSVNSNPNSPYDSLAGTPPPAYSPSEDGNSNNPNDGGQLLDAQMGDVAQVSYSEPAFWASIAYYELNCRVGEVFHCNNNSVIVDGFTNPSNNSDRCCLGQLSNVNRNSTIENTRRHIGKGVHLYYVTGEVYAECLSDSAIFVQSRNCNYHHGFHPSTVCKIPPGCSLKIFNNQEFAQLLSQSVNNGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNAISSVS

Target ORF information:

RefSeq Version	NM_057669.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mothers against dpp, transcript variant A (Mad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057669.3

ORF Insert Sequence:

ATGGACACCG ACGATGTGGA ATCGAACACA AGCAGCGCGA TGTCCACACT GGGCTCGCTA 
TTCTCCTTCA CATCGCCGGC GGTGAAGAAG CTGCTGGGCT GGAAACAGGG TGACGAAGAG 
GAGAAGTGGG CGGAGAAGGC CGTCGACAGT CTGGTGAAGA AGTTGAAGAA GCGCAAGGGC 
GCCATCGAGG AGCTGGAGCG TGCGCTCTCC TGTCCCGGTC AGCCCTCGAA GTGTGTCACC 
ATTCCACGCT CGCTGGACGG ACGATTACAG GTCTCCCATC GCAAGGGTCT GCCGCATGTG 
ATCTACTGCC GCGTGTGGCG CTGGCCAGAT CTGCAGTCGC ACCACGAACT GAAGCCACTC 
GAGCTGTGCC AGTATCCGTT CAGCGCCAAG CAGAAGGAGG TGTGCATCAA TCCGTATCAC 
TATAAGCGCG TGGAGAGTCC GGTGCTCCCG CCAGTACTCG TTCCTCGCCA CTCGGAATTC 
GCGCCCGGTC ACTCGATGCT GCAGTTCAAC CATGTGGCCG AGCCCAGTAT GCCGCACAAT 
GTGAGCTACT CGAACAGTGG ATTCAACTCG CACAGCTTGA GCACCAGCAA CACATCGGTG 
GGCAGTCCGA GTTCCGTCAA CTCCAATCCC AATTCGCCGT ACGACAGCTT GGCGGGAACA 
CCGCCACCCG CCTACAGTCC CTCGGAGGAT GGCAACTCCA ACAATCCGAA CGACGGTGGC 
CAGCTGCTGG ATGCTCAGAT GGGCGATGTT GCCCAGGTTA GCTATTCGGA GCCCGCCTTC 
TGGGCGTCGA TAGCCTACTA CGAGCTAAAT TGCCGCGTGG GCGAGGTGTT CCACTGCAAC 
AACAACTCCG TGATCGTTGA CGGTTTCACG AATCCTTCCA ACAACTCGGA CCGCTGCTGT 
CTCGGCCAGC TGAGCAACGT GAACAGGAAT AGCACCATCG AGAACACACG CCGTCATATA 
GGCAAGGGTG TTCATTTATA CTATGTGACC GGCGAGGTCT ACGCCGAATG CCTATCCGAC 
TCCGCCATTT TCGTGCAGTC GCGCAACTGC AACTACCACC ACGGATTCCA TCCGAGCACC 
GTGTGCAAAA TACCGCCGGG CTGCTCGCTG AAGATCTTCA ACAATCAGGA ATTTGCTCAG 
CTGCTGTCGC AGTCGGTGAA CAATGGATTC GAGGCCGTCT ACGAGCTGAC AAAGATGTGC 
ACGATCCGGA TGTCGTTCGT GAAGGGCTGG GGTGCGGAGT ATCATCGCCA GGATGTGACC 
TCGACGCCCT GTTGGATTGA AATCCATCTG CACGGGCCGC TCCAGTGGCT GGACAAGGTG 
CTCACCCAAA TGGGCTCTCC GCATAATGCA ATTAGTTCGG TATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mad ( NM_001273063.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259992.1 Drosophila melanogaster mothers against dpp, transcript variant B (Mad), mRNA.

Price & Availability↑

CloneID	OFa16145
Clone ID Related Accession (Same CDS sequence)	NM_001273063.1
Accession Version	NM_001273063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mothers against dpp, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACTATC CGCCCGCTGA TAGTTATACC GGTTATCGGG TGACTCCACC CCAAATCAAC 
GGCGGCACCA CCACCACCAC CACCATCAGC AGCGGCAGCA GCAACAACAA CAACATCCAC 
CACATCAACA ATAACAATAA CCACATCTGC AGCAGTGGCA GCAGCAACAA CCTCAATTTG 
ACGACAGCAA TCAGCGGCAG CAGCAACAGA ATGGACACCG ACGATGTGGA ATCGAACACA 
AGCAGCGCGA TGTCCACACT GGGCTCGCTA TTCTCCTTCA CATCGCCGGC GGTGAAGAAG 
CTGCTGGGCT GGAAACAGGG TGACGAAGAG GAGAAGTGGG CGGAGAAGGC CGTCGACAGT 
CTGGTGAAGA AGTTGAAGAA GCGCAAGGGC GCCATCGAGG AGCTGGAGCG TGCGCTCTCC 
TGTCCCGGTC AGCCCTCGAA GTGTGTCACC ATTCCACGCT CGCTGGACGG ACGATTACAG 
GTCTCCCATC GCAAGGGTCT GCCGCATGTG ATCTACTGCC GCGTGTGGCG CTGGCCAGAT 
CTGCAGTCGC ACCACGAACT GAAGCCACTC GAGCTGTGCC AGTATCCGTT CAGCGCCAAG 
CAGAAGGAGG TGTGCATCAA TCCGTATCAC TATAAGCGCG TGGAGAGTCC GGTGCTCCCG 
CCAGTACTCG TTCCTCGCCA CTCGGAATTC GCGCCCGGTC ACTCGATGCT GCAGTTCAAC 
CATGTGGCCG AGCCCAGTAT GCCGCACAAT GTGAGCTACT CGAACAGTGG ATTCAACTCG 
CACAGCTTGA GCACCAGCAA CACATCGGTG GGCAGTCCGA GTTCCGTCAA CTCCAATCCC 
AATTCGCCGT ACGACAGCTT GGCGGGAACA CCGCCACCCG CCTACAGTCC CTCGGAGGAT 
GGCAACTCCA ACAATCCGAA CGACGGTGGC CAGCTGCTGG ATGCTCAGAT GGGCGATGTT 
GCCCAGGTTA GCTATTCGGA GCCCGCCTTC TGGGCGTCGA TAGCCTACTA CGAGCTAAAT 
TGCCGCGTGG GCGAGGTGTT CCACTGCAAC AACAACTCCG TGATCGTTGA CGGTTTCACG 
AATCCTTCCA ACAACTCGGA CCGCTGCTGT CTCGGCCAGC TGAGCAACGT GAACAGGAAT 
AGCACCATCG AGAACACACG CCGTCATATA GGCAAGGGTG TTCATTTATA CTATGTGACC 
GGCGAGGTCT ACGCCGAATG CCTATCCGAC TCCGCCATTT TCGTGCAGTC GCGCAACTGC 
AACTACCACC ACGGATTCCA TCCGAGCACC GTGTGCAAAA TACCGCCGGG CTGCTCGCTG 
AAGATCTTCA ACAATCAGGA ATTTGCTCAG CTGCTGTCGC AGTCGGTGAA CAATGGATTC 
GAGGCCGTCT ACGAGCTGAC AAAGATGTGC ACGATCCGGA TGTCGTTCGT GAAGGGCTGG 
GGTGCGGAGT ATCATCGCCA GGATGTGACC TCGACGCCCT GTTGGATTGA AATCCATCTG 
CACGGGCCGC TCCAGTGGCT GGACAAGGTG CTCACCCAAA TGGGCTCTCC GCATAATGCA 
ATTAGTTCGG TATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259992.1
CDS	322..1899
Translation	MYYPPADSYTGYRVTPPQINGGTTTTTTISSGSSNNNNIHHINNNNNHICSSGSSNNLNLTTAISGSSNRMDTDDVESNTSSAMSTLGSLFSFTSPAVKKLLGWKQGDEEEKWAEKAVDSLVKKLKKRKGAIEELERALSCPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLELCQYPFSAKQKEVCINPYHYKRVESPVLPPVLVPRHSEFAPGHSMLQFNHVAEPSMPHNVSYSNSGFNSHSLSTSNTSVGSPSSVNSNPNSPYDSLAGTPPPAYSPSEDGNSNNPNDGGQLLDAQMGDVAQVSYSEPAFWASIAYYELNCRVGEVFHCNNNSVIVDGFTNPSNNSDRCCLGQLSNVNRNSTIENTRRHIGKGVHLYYVTGEVYAECLSDSAIFVQSRNCNYHHGFHPSTVCKIPPGCSLKIFNNQEFAQLLSQSVNNGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNAISSVS

Target ORF information:

RefSeq Version	NM_001273063.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mothers against dpp, transcript variant B (Mad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273063.1

ORF Insert Sequence:

ATGTACTATC CGCCCGCTGA TAGTTATACC GGTTATCGGG TGACTCCACC CCAAATCAAC 
GGCGGCACCA CCACCACCAC CACCATCAGC AGCGGCAGCA GCAACAACAA CAACATCCAC 
CACATCAACA ATAACAATAA CCACATCTGC AGCAGTGGCA GCAGCAACAA CCTCAATTTG 
ACGACAGCAA TCAGCGGCAG CAGCAACAGA ATGGACACCG ACGATGTGGA ATCGAACACA 
AGCAGCGCGA TGTCCACACT GGGCTCGCTA TTCTCCTTCA CATCGCCGGC GGTGAAGAAG 
CTGCTGGGCT GGAAACAGGG TGACGAAGAG GAGAAGTGGG CGGAGAAGGC CGTCGACAGT 
CTGGTGAAGA AGTTGAAGAA GCGCAAGGGC GCCATCGAGG AGCTGGAGCG TGCGCTCTCC 
TGTCCCGGTC AGCCCTCGAA GTGTGTCACC ATTCCACGCT CGCTGGACGG ACGATTACAG 
GTCTCCCATC GCAAGGGTCT GCCGCATGTG ATCTACTGCC GCGTGTGGCG CTGGCCAGAT 
CTGCAGTCGC ACCACGAACT GAAGCCACTC GAGCTGTGCC AGTATCCGTT CAGCGCCAAG 
CAGAAGGAGG TGTGCATCAA TCCGTATCAC TATAAGCGCG TGGAGAGTCC GGTGCTCCCG 
CCAGTACTCG TTCCTCGCCA CTCGGAATTC GCGCCCGGTC ACTCGATGCT GCAGTTCAAC 
CATGTGGCCG AGCCCAGTAT GCCGCACAAT GTGAGCTACT CGAACAGTGG ATTCAACTCG 
CACAGCTTGA GCACCAGCAA CACATCGGTG GGCAGTCCGA GTTCCGTCAA CTCCAATCCC 
AATTCGCCGT ACGACAGCTT GGCGGGAACA CCGCCACCCG CCTACAGTCC CTCGGAGGAT 
GGCAACTCCA ACAATCCGAA CGACGGTGGC CAGCTGCTGG ATGCTCAGAT GGGCGATGTT 
GCCCAGGTTA GCTATTCGGA GCCCGCCTTC TGGGCGTCGA TAGCCTACTA CGAGCTAAAT 
TGCCGCGTGG GCGAGGTGTT CCACTGCAAC AACAACTCCG TGATCGTTGA CGGTTTCACG 
AATCCTTCCA ACAACTCGGA CCGCTGCTGT CTCGGCCAGC TGAGCAACGT GAACAGGAAT 
AGCACCATCG AGAACACACG CCGTCATATA GGCAAGGGTG TTCATTTATA CTATGTGACC 
GGCGAGGTCT ACGCCGAATG CCTATCCGAC TCCGCCATTT TCGTGCAGTC GCGCAACTGC 
AACTACCACC ACGGATTCCA TCCGAGCACC GTGTGCAAAA TACCGCCGGG CTGCTCGCTG 
AAGATCTTCA ACAATCAGGA ATTTGCTCAG CTGCTGTCGC AGTCGGTGAA CAATGGATTC 
GAGGCCGTCT ACGAGCTGAC AAAGATGTGC ACGATCCGGA TGTCGTTCGT GAAGGGCTGG 
GGTGCGGAGT ATCATCGCCA GGATGTGACC TCGACGCCCT GTTGGATTGA AATCCATCTG 
CACGGGCCGC TCCAGTGGCT GGACAAGGTG CTCACCCAAA TGGGCTCTCC GCATAATGCA 
ATTAGTTCGG TATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mad cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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